AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0184680


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0184680 MtrunA17_Chr1g0205951 94.472 199 11 0 1 199 1 199 2.10e-137 382
Msa0184680 MtrunA17_Chr5g0394151 63.902 205 64 2 1 199 1 201 5.20e-96 277
Msa0184680 MtrunA17_Chr1g0191761 36.943 157 96 3 20 173 26 182 8.28e-32 114
Msa0184680 MtrunA17_Chr7g0272941 38.889 144 84 3 19 159 17 159 3.24e-31 112
Msa0184680 MtrunA17_Chr1g0191781 32.727 165 105 3 11 169 6 170 1.18e-27 103
Msa0184680 MtrunA17_Chr1g0191771 34.328 134 87 1 31 163 46 179 2.74e-23 92.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0184680 AT3G06035.1 58.659 179 70 2 25 199 21 199 3.29e-72 217
Msa0184680 AT5G19250.1 61.078 167 63 1 24 188 19 185 1.92e-68 207
Msa0184680 AT1G54860.1 41.808 177 85 4 1 168 2 169 1.06e-46 152
Msa0184680 AT5G19240.1 45.349 172 88 3 23 192 20 187 2.24e-46 152
Msa0184680 AT5G19230.2 45.562 169 80 3 1 169 1 157 4.04e-41 138
Msa0184680 AT5G19230.1 45.562 169 80 3 1 169 1 157 4.04e-41 138

Find 42 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAATTGACATTTAGCTAAA+AGG 0.190847 1_4:-74257048 None:intergenic
CCAAGTTCTCTCTATTCTTC+TGG 0.209568 1_4:+74256226 Msa0184680:CDS
GTTATGCCTGCCTGTGTTCC+TGG 0.264326 1_4:+74257097 Msa0184680:CDS
TCCAACTTTGGAAATCAAAT+TGG 0.265283 1_4:-74257290 None:intergenic
TGAGAGAGATAATCTTTATC+AGG 0.299934 1_4:+74256856 Msa0184680:CDS
TGACATTTAGCTAAAAGGTT+TGG 0.301742 1_4:-74257043 None:intergenic
AGCTAAAAGGTTTGGGTAGT+TGG 0.318984 1_4:-74257035 None:intergenic
GTATTGGCTCAGAAGATAAC+TGG 0.333128 1_4:+74257203 Msa0184680:CDS
GACATTTAGCTAAAAGGTTT+GGG 0.340607 1_4:-74257042 None:intergenic
CCAGAAGAATAGAGAGAACT+TGG 0.347419 1_4:-74256226 None:intergenic
GCAATAAATCATTCCAACTT+TGG 0.358521 1_4:-74257302 None:intergenic
TACATGGTTGATTCTTGAAT+TGG 0.360348 1_4:-74256964 None:intergenic
ATTGGCAGCATTAGAACTAT+AGG 0.374058 1_4:-74257272 None:intergenic
GCCAATTTGATTTCCAAAGT+TGG 0.375408 1_4:+74257289 Msa0184680:CDS
TTGGCAGCATTAGAACTATA+GGG 0.380106 1_4:-74257271 None:intergenic
CATCCTCTAGGTATCATAAC+AGG 0.398840 1_4:-74257354 None:intergenic
TCACCGTCATCACATTTAAC+TGG 0.400596 1_4:-74256288 None:intergenic
GACACACAGTATACAGGAAT+TGG 0.421140 1_4:+74257181 Msa0184680:CDS
TGCTCAATTCTCTTGTTAGC+TGG 0.427277 1_4:+74257322 Msa0184680:CDS
CTCAACGACACACAGTATAC+AGG 0.481619 1_4:+74257175 Msa0184680:CDS
TTGACCACTGACACTCCAAC+AGG 0.483770 1_4:+74257238 Msa0184680:CDS
TTTGGGTAGTTGGAGAATTG+AGG 0.485525 1_4:-74257025 None:intergenic
ATTGGCTATTTCTGATACAA+AGG 0.497292 1_4:+74257066 Msa0184680:CDS
ACGAGGCTTGAAGCTAGGCC+AGG 0.504069 1_4:-74257115 None:intergenic
TGAAGCTAGGCCAGGAACAC+AGG 0.509872 1_4:-74257107 None:intergenic
GCTAGGCCAGGAACACAGGC+AGG 0.513127 1_4:-74257103 None:intergenic
GAGAATTGAGGTTCAGTACC+TGG 0.522201 1_4:-74257013 None:intergenic
GCTATTTCTGATACAAAGGA+TGG 0.531181 1_4:+74257070 Msa0184680:CDS
CAGTATACAGGAATTGGTAT+TGG 0.536270 1_4:+74257187 Msa0184680:CDS
GCACCTGTGGTATTTGTACA+TGG 0.542966 1_4:-74256980 None:intergenic
TCGTGAAGTTAGTGAGGACG+AGG 0.562843 1_4:-74257132 None:intergenic
TGAGGACGAGGCTTGAAGCT+AGG 0.566605 1_4:-74257120 None:intergenic
GTTTCCTGTTGGAGTGTCAG+TGG 0.576612 1_4:-74257242 None:intergenic
GAGATTTCGTGAAGTTAGTG+AGG 0.582648 1_4:-74257138 None:intergenic
GAGAGAGATAATCTTTATCA+GGG 0.592178 1_4:+74256857 Msa0184680:CDS
TATCCAGTTAAATGTGATGA+CGG 0.600716 1_4:+74256285 Msa0184680:CDS
TGGTACTGTATTAGCACCTG+TGG 0.607702 1_4:-74256993 None:intergenic
TGGCAGCATTAGAACTATAG+GGG 0.612217 1_4:-74257270 None:intergenic
ACAGGTGCTAATACAGTACC+AGG 0.621692 1_4:+74256995 Msa0184680:CDS
CAGAAGAATAGAGAGAACTT+GGG 0.624874 1_4:-74256225 None:intergenic
CAACCATGTACAAATACCAC+AGG 0.631778 1_4:+74256977 Msa0184680:CDS
GGCAGCATTAGAACTATAGG+GGG 0.666389 1_4:-74257269 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTGACCAATACAAAATA+TGG - chr1_4:74256369-74256388 None:intergenic 20.0%
!!! ATTTTGACCAATACAAAATA+TGG - chr1_4:74256369-74256388 None:intergenic 20.0%
!! TCTTAGACCATATTTTGTAT+TGG + chr1_4:74256359-74256378 Msa0184680:intron 25.0%
! ATTCATGAGATTAATGTCAT+TGG + chr1_4:74256434-74256453 Msa0184680:intron 25.0%
! ATTCATGAGATTAATGTCAT+TGG + chr1_4:74256434-74256453 Msa0184680:intron 25.0%
!! TCTTAGACCATATTTTGTAT+TGG + chr1_4:74256359-74256378 Msa0184680:intron 25.0%
! ATTCATGAGATTAATGTCAT+TGG + chr1_4:74256434-74256453 Msa0184680:intron 25.0%
! ATTCATGAGATTAATGTCAT+TGG + chr1_4:74256434-74256453 Msa0184680:intron 25.0%
TATCCAGTTAAATGTGATGA+CGG + chr1_4:74256285-74256304 Msa0184680:CDS 30.0%
TGTCAGTGAACAAAATGATT+TGG - chr1_4:74256728-74256747 None:intergenic 30.0%
TTTCAGCTTCATTGTGTATA+TGG - chr1_4:74256794-74256813 None:intergenic 30.0%
TGCAGAAAAAACATTGAACA+GGG - chr1_4:74256834-74256853 None:intergenic 30.0%
TGAGAGAGATAATCTTTATC+AGG + chr1_4:74256856-74256875 Msa0184680:CDS 30.0%
GAGAGAGATAATCTTTATCA+GGG + chr1_4:74256857-74256876 Msa0184680:CDS 30.0%
! TACATGGTTGATTCTTGAAT+TGG - chr1_4:74256967-74256986 None:intergenic 30.0%
GACATTTAGCTAAAAGGTTT+GGG - chr1_4:74257045-74257064 None:intergenic 30.0%
TGACATTTAGCTAAAAGGTT+TGG - chr1_4:74257046-74257065 None:intergenic 30.0%
CCAATTGACATTTAGCTAAA+AGG - chr1_4:74257051-74257070 None:intergenic 30.0%
!! CCTTTTAGCTAAATGTCAAT+TGG + chr1_4:74257048-74257067 Msa0184680:CDS 30.0%
!! ATTGGCTATTTCTGATACAA+AGG + chr1_4:74257066-74257085 Msa0184680:CDS 30.0%
! TCCAACTTTGGAAATCAAAT+TGG - chr1_4:74257293-74257312 None:intergenic 30.0%
GCAATAAATCATTCCAACTT+TGG - chr1_4:74257305-74257324 None:intergenic 30.0%
TATCCAGTTAAATGTGATGA+CGG + chr1_4:74256285-74256304 Msa0184680:CDS 30.0%
TGTCAGTGAACAAAATGATT+TGG - chr1_4:74256728-74256747 None:intergenic 30.0%
TTTCAGCTTCATTGTGTATA+TGG - chr1_4:74256794-74256813 None:intergenic 30.0%
TGCAGAAAAAACATTGAACA+GGG - chr1_4:74256834-74256853 None:intergenic 30.0%
TGAGAGAGATAATCTTTATC+AGG + chr1_4:74256856-74256875 Msa0184680:CDS 30.0%
GAGAGAGATAATCTTTATCA+GGG + chr1_4:74256857-74256876 Msa0184680:CDS 30.0%
! TACATGGTTGATTCTTGAAT+TGG - chr1_4:74256967-74256986 None:intergenic 30.0%
GACATTTAGCTAAAAGGTTT+GGG - chr1_4:74257045-74257064 None:intergenic 30.0%
TGACATTTAGCTAAAAGGTT+TGG - chr1_4:74257046-74257065 None:intergenic 30.0%
CCAATTGACATTTAGCTAAA+AGG - chr1_4:74257051-74257070 None:intergenic 30.0%
!! CCTTTTAGCTAAATGTCAAT+TGG + chr1_4:74257048-74257067 Msa0184680:CDS 30.0%
!! ATTGGCTATTTCTGATACAA+AGG + chr1_4:74257066-74257085 Msa0184680:CDS 30.0%
! TCCAACTTTGGAAATCAAAT+TGG - chr1_4:74257293-74257312 None:intergenic 30.0%
GCAATAAATCATTCCAACTT+TGG - chr1_4:74257305-74257324 None:intergenic 30.0%
CAGAAGAATAGAGAGAACTT+GGG - chr1_4:74256228-74256247 None:intergenic 35.0%
CTAAGCTTGAAATGAGTCAT+GGG - chr1_4:74256507-74256526 None:intergenic 35.0%
ATGCCTCTTAAAACATTCAC+TGG + chr1_4:74256627-74256646 Msa0184680:intron 35.0%
!! TGTCCAGTGAATGTTTTAAG+AGG - chr1_4:74256633-74256652 None:intergenic 35.0%
CTGCAGAAAAAACATTGAAC+AGG - chr1_4:74256835-74256854 None:intergenic 35.0%
!! GCTATTTCTGATACAAAGGA+TGG + chr1_4:74257070-74257089 Msa0184680:CDS 35.0%
CAGTATACAGGAATTGGTAT+TGG + chr1_4:74257187-74257206 Msa0184680:CDS 35.0%
TTGGCAGCATTAGAACTATA+GGG - chr1_4:74257274-74257293 None:intergenic 35.0%
ATTGGCAGCATTAGAACTAT+AGG - chr1_4:74257275-74257294 None:intergenic 35.0%
! GCCAATTTGATTTCCAAAGT+TGG + chr1_4:74257289-74257308 Msa0184680:CDS 35.0%
CAGAAGAATAGAGAGAACTT+GGG - chr1_4:74256228-74256247 None:intergenic 35.0%
CTAAGCTTGAAATGAGTCAT+GGG - chr1_4:74256507-74256526 None:intergenic 35.0%
ATGCCTCTTAAAACATTCAC+TGG + chr1_4:74256627-74256646 Msa0184680:intron 35.0%
!! TGTCCAGTGAATGTTTTAAG+AGG - chr1_4:74256633-74256652 None:intergenic 35.0%
CTGCAGAAAAAACATTGAAC+AGG - chr1_4:74256835-74256854 None:intergenic 35.0%
!! GCTATTTCTGATACAAAGGA+TGG + chr1_4:74257070-74257089 Msa0184680:CDS 35.0%
CAGTATACAGGAATTGGTAT+TGG + chr1_4:74257187-74257206 Msa0184680:CDS 35.0%
TTGGCAGCATTAGAACTATA+GGG - chr1_4:74257274-74257293 None:intergenic 35.0%
ATTGGCAGCATTAGAACTAT+AGG - chr1_4:74257275-74257294 None:intergenic 35.0%
! GCCAATTTGATTTCCAAAGT+TGG + chr1_4:74257289-74257308 Msa0184680:CDS 35.0%
CCAGAAGAATAGAGAGAACT+TGG - chr1_4:74256229-74256248 None:intergenic 40.0%
CCAAGTTCTCTCTATTCTTC+TGG + chr1_4:74256226-74256245 Msa0184680:CDS 40.0%
TCACCGTCATCACATTTAAC+TGG - chr1_4:74256291-74256310 None:intergenic 40.0%
GCTAAGCTTGAAATGAGTCA+TGG - chr1_4:74256508-74256527 None:intergenic 40.0%
TTGAGTAATGCACAGATGAC+AGG - chr1_4:74256658-74256677 None:intergenic 40.0%
! TCTCAGTTGTGACCTTTTTC+TGG + chr1_4:74256749-74256768 Msa0184680:intron 40.0%
ACAGGGTAAGTGTAGTGTAA+TGG - chr1_4:74256817-74256836 None:intergenic 40.0%
!! GGTCAGCTATTTTATCAGCA+AGG - chr1_4:74256946-74256965 None:intergenic 40.0%
CAACCATGTACAAATACCAC+AGG + chr1_4:74256977-74256996 Msa0184680:CDS 40.0%
TTTGGGTAGTTGGAGAATTG+AGG - chr1_4:74257028-74257047 None:intergenic 40.0%
AGCTAAAAGGTTTGGGTAGT+TGG - chr1_4:74257038-74257057 None:intergenic 40.0%
GAGATTTCGTGAAGTTAGTG+AGG - chr1_4:74257141-74257160 None:intergenic 40.0%
GACACACAGTATACAGGAAT+TGG + chr1_4:74257181-74257200 Msa0184680:CDS 40.0%
!! GTATTGGCTCAGAAGATAAC+TGG + chr1_4:74257203-74257222 Msa0184680:CDS 40.0%
TGGCAGCATTAGAACTATAG+GGG - chr1_4:74257273-74257292 None:intergenic 40.0%
TGCTCAATTCTCTTGTTAGC+TGG + chr1_4:74257322-74257341 Msa0184680:CDS 40.0%
CCAGAAGAATAGAGAGAACT+TGG - chr1_4:74256229-74256248 None:intergenic 40.0%
CCAAGTTCTCTCTATTCTTC+TGG + chr1_4:74256226-74256245 Msa0184680:CDS 40.0%
TCACCGTCATCACATTTAAC+TGG - chr1_4:74256291-74256310 None:intergenic 40.0%
GCTAAGCTTGAAATGAGTCA+TGG - chr1_4:74256508-74256527 None:intergenic 40.0%
TTGAGTAATGCACAGATGAC+AGG - chr1_4:74256658-74256677 None:intergenic 40.0%
! TCTCAGTTGTGACCTTTTTC+TGG + chr1_4:74256749-74256768 Msa0184680:intron 40.0%
ACAGGGTAAGTGTAGTGTAA+TGG - chr1_4:74256817-74256836 None:intergenic 40.0%
!! GGTCAGCTATTTTATCAGCA+AGG - chr1_4:74256946-74256965 None:intergenic 40.0%
CAACCATGTACAAATACCAC+AGG + chr1_4:74256977-74256996 Msa0184680:CDS 40.0%
TTTGGGTAGTTGGAGAATTG+AGG - chr1_4:74257028-74257047 None:intergenic 40.0%
AGCTAAAAGGTTTGGGTAGT+TGG - chr1_4:74257038-74257057 None:intergenic 40.0%
GAGATTTCGTGAAGTTAGTG+AGG - chr1_4:74257141-74257160 None:intergenic 40.0%
GACACACAGTATACAGGAAT+TGG + chr1_4:74257181-74257200 Msa0184680:CDS 40.0%
!! GTATTGGCTCAGAAGATAAC+TGG + chr1_4:74257203-74257222 Msa0184680:CDS 40.0%
TGGCAGCATTAGAACTATAG+GGG - chr1_4:74257273-74257292 None:intergenic 40.0%
TGCTCAATTCTCTTGTTAGC+TGG + chr1_4:74257322-74257341 Msa0184680:CDS 40.0%
TGTGCATGTGTGCCAGAAAA+AGG - chr1_4:74256764-74256783 None:intergenic 45.0%
GCACCTGTGGTATTTGTACA+TGG - chr1_4:74256983-74257002 None:intergenic 45.0%
TGGTACTGTATTAGCACCTG+TGG - chr1_4:74256996-74257015 None:intergenic 45.0%
ACAGGTGCTAATACAGTACC+AGG + chr1_4:74256995-74257014 Msa0184680:CDS 45.0%
GAGAATTGAGGTTCAGTACC+TGG - chr1_4:74257016-74257035 None:intergenic 45.0%
CTCAACGACACACAGTATAC+AGG + chr1_4:74257175-74257194 Msa0184680:CDS 45.0%
GGCAGCATTAGAACTATAGG+GGG - chr1_4:74257272-74257291 None:intergenic 45.0%
TGTGCATGTGTGCCAGAAAA+AGG - chr1_4:74256764-74256783 None:intergenic 45.0%
GCACCTGTGGTATTTGTACA+TGG - chr1_4:74256983-74257002 None:intergenic 45.0%
TGGTACTGTATTAGCACCTG+TGG - chr1_4:74256996-74257015 None:intergenic 45.0%
ACAGGTGCTAATACAGTACC+AGG + chr1_4:74256995-74257014 Msa0184680:CDS 45.0%
GAGAATTGAGGTTCAGTACC+TGG - chr1_4:74257016-74257035 None:intergenic 45.0%
CTCAACGACACACAGTATAC+AGG + chr1_4:74257175-74257194 Msa0184680:CDS 45.0%
GGCAGCATTAGAACTATAGG+GGG - chr1_4:74257272-74257291 None:intergenic 45.0%
TCATCGCAGTCGCGAGATTA+GGG - chr1_4:74256581-74256600 None:intergenic 50.0%
TTCATCGCAGTCGCGAGATT+AGG - chr1_4:74256582-74256601 None:intergenic 50.0%
TCGTGAAGTTAGTGAGGACG+AGG - chr1_4:74257135-74257154 None:intergenic 50.0%
TTGACCACTGACACTCCAAC+AGG + chr1_4:74257238-74257257 Msa0184680:CDS 50.0%
! GTTTCCTGTTGGAGTGTCAG+TGG - chr1_4:74257245-74257264 None:intergenic 50.0%
TAGGGGGCAAAGTTTCCTGT+TGG - chr1_4:74257256-74257275 None:intergenic 50.0%
TCATCGCAGTCGCGAGATTA+GGG - chr1_4:74256581-74256600 None:intergenic 50.0%
TTCATCGCAGTCGCGAGATT+AGG - chr1_4:74256582-74256601 None:intergenic 50.0%
TCGTGAAGTTAGTGAGGACG+AGG - chr1_4:74257135-74257154 None:intergenic 50.0%
TTGACCACTGACACTCCAAC+AGG + chr1_4:74257238-74257257 Msa0184680:CDS 50.0%
! GTTTCCTGTTGGAGTGTCAG+TGG - chr1_4:74257245-74257264 None:intergenic 50.0%
TAGGGGGCAAAGTTTCCTGT+TGG - chr1_4:74257256-74257275 None:intergenic 50.0%
GTTATGCCTGCCTGTGTTCC+TGG + chr1_4:74257097-74257116 Msa0184680:CDS 55.0%
TGAAGCTAGGCCAGGAACAC+AGG - chr1_4:74257110-74257129 None:intergenic 55.0%
TGAGGACGAGGCTTGAAGCT+AGG - chr1_4:74257123-74257142 None:intergenic 55.0%
GTTATGCCTGCCTGTGTTCC+TGG + chr1_4:74257097-74257116 Msa0184680:CDS 55.0%
TGAAGCTAGGCCAGGAACAC+AGG - chr1_4:74257110-74257129 None:intergenic 55.0%
TGAGGACGAGGCTTGAAGCT+AGG - chr1_4:74257123-74257142 None:intergenic 55.0%
ACGAGGCTTGAAGCTAGGCC+AGG - chr1_4:74257118-74257137 None:intergenic 60.0%
ACGAGGCTTGAAGCTAGGCC+AGG - chr1_4:74257118-74257137 None:intergenic 60.0%
GCTAGGCCAGGAACACAGGC+AGG - chr1_4:74257106-74257125 None:intergenic 65.0%
GCTAGGCCAGGAACACAGGC+AGG - chr1_4:74257106-74257125 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 74256216 74257363 74256216 ID=Msa0184680;Name=Msa0184680
chr1_4 mRNA 74256216 74257363 74256216 ID=Msa0184680-mRNA-1;Parent=Msa0184680;Name=Msa0184680-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|0|0|1|0|0.5|2|0|199
chr1_4 exon 74256216 74256306 74256216 ID=Msa0184680-mRNA-1:exon:25272;Parent=Msa0184680-mRNA-1
chr1_4 exon 74256855 74257363 74256855 ID=Msa0184680-mRNA-1:exon:25273;Parent=Msa0184680-mRNA-1
chr1_4 CDS 74256216 74256306 74256216 ID=Msa0184680-mRNA-1:cds;Parent=Msa0184680-mRNA-1
chr1_4 CDS 74256855 74257363 74256855 ID=Msa0184680-mRNA-1:cds;Parent=Msa0184680-mRNA-1
Gene Sequence

>Msa0184680

ATGGTGTTGCCCAAGTTCTCTCTATTCTTCTGGATTCTTTCTTCTTCATGTGTTATTCTCTTGATGAAATATCCAGTTAAATGTGATGACGATGAGAGAGATAATCTTTATCAGGGTATCAACAAGTATCGAGCATCATTAAACTTGAAATCTCTCACAAAAAATAAAAATGCAGATTGCCTTGCTGATAAAATAGCTGACCAATTCAAGAATCAACCATGTACAAATACCACAGGTGCTAATACAGTACCAGGTACTGAACCTCAATTCTCCAACTACCCAAACCTTTTAGCTAAATGTCAATTGGCTATTTCTGATACAAAGGATGGAGCTGTTATGCCTGCCTGTGTTCCTGGCCTAGCTTCAAGCCTCGTCCTCACTAACTTCACGAAATCTCTCTACTCACAGAATCTCAACGACACACAGTATACAGGAATTGGTATTGGCTCAGAAGATAACTGGATTGTCGTTGTCTTGACCACTGACACTCCAACAGGAAACTTTGCCCCCTATAGTTCTAATGCTGCCAATTTGATTTCCAAAGTTGGAATGATTTATTGCTCAATTCTCTTGTTAGCTGGCAAAATTTTCCTGTTATGA

Protein sequence

>Msa0184680

MVLPKFSLFFWILSSSCVILLMKYPVKCDDDERDNLYQGINKYRASLNLKSLTKNKNADCLADKIADQFKNQPCTNTTGANTVPGTEPQFSNYPNLLAKCQLAISDTKDGAVMPACVPGLASSLVLTNFTKSLYSQNLNDTQYTGIGIGSEDNWIVVVLTTDTPTGNFAPYSSNAANLISKVGMIYCSILLLAGKIFLL*