AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0688820


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0688820 MtrunA17_Chr1g0205951 94.472 199 11 0 1 199 1 199 2.10e-137 382
Msa0688820 MtrunA17_Chr5g0394151 63.902 205 64 2 1 199 1 201 5.20e-96 277
Msa0688820 MtrunA17_Chr1g0191761 36.943 157 96 3 20 173 26 182 8.28e-32 114
Msa0688820 MtrunA17_Chr7g0272941 38.889 144 84 3 19 159 17 159 3.24e-31 112
Msa0688820 MtrunA17_Chr1g0191781 32.727 165 105 3 11 169 6 170 1.18e-27 103
Msa0688820 MtrunA17_Chr1g0191771 34.328 134 87 1 31 163 46 179 2.74e-23 92.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0688820 AT3G06035.1 58.659 179 70 2 25 199 21 199 3.29e-72 217
Msa0688820 AT5G19250.1 61.078 167 63 1 24 188 19 185 1.92e-68 207
Msa0688820 AT1G54860.1 41.808 177 85 4 1 168 2 169 1.06e-46 152
Msa0688820 AT5G19240.1 45.349 172 88 3 23 192 20 187 2.24e-46 152
Msa0688820 AT5G19230.2 45.562 169 80 3 1 169 1 157 4.04e-41 138
Msa0688820 AT5G19230.1 45.562 169 80 3 1 169 1 157 4.04e-41 138

Find 42 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAATTGACATTTAGCTAAA+AGG 0.190847 4_4:+55079701 None:intergenic
CCAAGTTCTCTCTATTCTTC+TGG 0.209568 4_4:-55080523 Msa0688820:CDS
GTTATGCCTGCCTGTGTTCC+TGG 0.264326 4_4:-55079652 Msa0688820:CDS
TCCAACTTTGGAAATCAAAT+TGG 0.265283 4_4:+55079459 None:intergenic
TGAGAGAGATAATCTTTATC+AGG 0.299934 4_4:-55079893 Msa0688820:CDS
TGACATTTAGCTAAAAGGTT+TGG 0.301742 4_4:+55079706 None:intergenic
AGCTAAAAGGTTTGGGTAGT+TGG 0.318984 4_4:+55079714 None:intergenic
GTATTGGCTCAGAAGATAAC+TGG 0.333128 4_4:-55079546 Msa0688820:CDS
GACATTTAGCTAAAAGGTTT+GGG 0.340607 4_4:+55079707 None:intergenic
CCAGAAGAATAGAGAGAACT+TGG 0.347419 4_4:+55080523 None:intergenic
GCAATAAATCATTCCAACTT+TGG 0.358521 4_4:+55079447 None:intergenic
TACATGGTTGATTCTTGAAT+TGG 0.360348 4_4:+55079785 None:intergenic
ATTGGCAGCATTAGAACTAT+AGG 0.374058 4_4:+55079477 None:intergenic
GCCAATTTGATTTCCAAAGT+TGG 0.375408 4_4:-55079460 Msa0688820:CDS
TTGGCAGCATTAGAACTATA+GGG 0.380106 4_4:+55079478 None:intergenic
CATCCTCTAGGTATCATAAC+AGG 0.398840 4_4:+55079395 None:intergenic
TCACCGTCATCACATTTAAC+TGG 0.400596 4_4:+55080461 None:intergenic
GACACACAGTATACAGGAAT+TGG 0.421140 4_4:-55079568 Msa0688820:CDS
TGCTCAATTCTCTTGTTAGC+TGG 0.427277 4_4:-55079427 Msa0688820:CDS
CTCAACGACACACAGTATAC+AGG 0.481619 4_4:-55079574 Msa0688820:CDS
TTGACCACTGACACTCCAAC+AGG 0.483770 4_4:-55079511 Msa0688820:CDS
TTTGGGTAGTTGGAGAATTG+AGG 0.485525 4_4:+55079724 None:intergenic
ATTGGCTATTTCTGATACAA+AGG 0.497292 4_4:-55079683 Msa0688820:CDS
ACGAGGCTTGAAGCTAGGCC+AGG 0.504069 4_4:+55079634 None:intergenic
TGAAGCTAGGCCAGGAACAC+AGG 0.509872 4_4:+55079642 None:intergenic
GCTAGGCCAGGAACACAGGC+AGG 0.513127 4_4:+55079646 None:intergenic
GAGAATTGAGGTTCAGTACC+TGG 0.522201 4_4:+55079736 None:intergenic
GCTATTTCTGATACAAAGGA+TGG 0.531181 4_4:-55079679 Msa0688820:CDS
CAGTATACAGGAATTGGTAT+TGG 0.536270 4_4:-55079562 Msa0688820:CDS
GCACCTGTGGTATTTGTACA+TGG 0.542966 4_4:+55079769 None:intergenic
TCGTGAAGTTAGTGAGGACG+AGG 0.562843 4_4:+55079617 None:intergenic
TGAGGACGAGGCTTGAAGCT+AGG 0.566605 4_4:+55079629 None:intergenic
GTTTCCTGTTGGAGTGTCAG+TGG 0.576612 4_4:+55079507 None:intergenic
GAGATTTCGTGAAGTTAGTG+AGG 0.582648 4_4:+55079611 None:intergenic
GAGAGAGATAATCTTTATCA+GGG 0.592178 4_4:-55079892 Msa0688820:CDS
TATCCAGTTAAATGTGATGA+CGG 0.600716 4_4:-55080464 Msa0688820:intron
TGGTACTGTATTAGCACCTG+TGG 0.607702 4_4:+55079756 None:intergenic
TGGCAGCATTAGAACTATAG+GGG 0.612217 4_4:+55079479 None:intergenic
ACAGGTGCTAATACAGTACC+AGG 0.621692 4_4:-55079754 Msa0688820:CDS
CAGAAGAATAGAGAGAACTT+GGG 0.624874 4_4:+55080524 None:intergenic
CAACCATGTACAAATACCAC+AGG 0.631778 4_4:-55079772 Msa0688820:CDS
GGCAGCATTAGAACTATAGG+GGG 0.666389 4_4:+55079480 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTGACCAATACAAAATA+TGG + chr4_4:55079561-55079580 None:intergenic 20.0%
!!! ATTTTGACCAATACAAAATA+TGG + chr4_4:55079561-55079580 None:intergenic 20.0%
!! TCTTAGACCATATTTTGTAT+TGG - chr4_4:55079551-55079570 Msa0688820:CDS 25.0%
! ATTCATGAGATTAATGTCAT+TGG - chr4_4:55079626-55079645 Msa0688820:CDS 25.0%
! ATTCATGAGATTAATGTCAT+TGG - chr4_4:55079626-55079645 Msa0688820:CDS 25.0%
!! TCTTAGACCATATTTTGTAT+TGG - chr4_4:55079551-55079570 Msa0688820:CDS 25.0%
! ATTCATGAGATTAATGTCAT+TGG - chr4_4:55079626-55079645 Msa0688820:CDS 25.0%
! ATTCATGAGATTAATGTCAT+TGG - chr4_4:55079626-55079645 Msa0688820:CDS 25.0%
TATCCAGTTAAATGTGATGA+CGG - chr4_4:55079477-55079496 Msa0688820:CDS 30.0%
TGTCAGTGAACAAAATGATT+TGG + chr4_4:55079920-55079939 None:intergenic 30.0%
TTTCAGCTTCATTGTGTATA+TGG + chr4_4:55079986-55080005 None:intergenic 30.0%
TGCAGAAAAAACATTGAACA+GGG + chr4_4:55080026-55080045 None:intergenic 30.0%
TGAGAGAGATAATCTTTATC+AGG - chr4_4:55080048-55080067 Msa0688820:intron 30.0%
GAGAGAGATAATCTTTATCA+GGG - chr4_4:55080049-55080068 Msa0688820:intron 30.0%
! TACATGGTTGATTCTTGAAT+TGG + chr4_4:55080159-55080178 None:intergenic 30.0%
GACATTTAGCTAAAAGGTTT+GGG + chr4_4:55080237-55080256 None:intergenic 30.0%
TGACATTTAGCTAAAAGGTT+TGG + chr4_4:55080238-55080257 None:intergenic 30.0%
CCAATTGACATTTAGCTAAA+AGG + chr4_4:55080243-55080262 None:intergenic 30.0%
!! CCTTTTAGCTAAATGTCAAT+TGG - chr4_4:55080240-55080259 Msa0688820:intron 30.0%
!! ATTGGCTATTTCTGATACAA+AGG - chr4_4:55080258-55080277 Msa0688820:intron 30.0%
! TCCAACTTTGGAAATCAAAT+TGG + chr4_4:55080485-55080504 None:intergenic 30.0%
GCAATAAATCATTCCAACTT+TGG + chr4_4:55080497-55080516 None:intergenic 30.0%
TATCCAGTTAAATGTGATGA+CGG - chr4_4:55079477-55079496 Msa0688820:CDS 30.0%
TGTCAGTGAACAAAATGATT+TGG + chr4_4:55079920-55079939 None:intergenic 30.0%
TTTCAGCTTCATTGTGTATA+TGG + chr4_4:55079986-55080005 None:intergenic 30.0%
TGCAGAAAAAACATTGAACA+GGG + chr4_4:55080026-55080045 None:intergenic 30.0%
TGAGAGAGATAATCTTTATC+AGG - chr4_4:55080048-55080067 Msa0688820:intron 30.0%
GAGAGAGATAATCTTTATCA+GGG - chr4_4:55080049-55080068 Msa0688820:intron 30.0%
! TACATGGTTGATTCTTGAAT+TGG + chr4_4:55080159-55080178 None:intergenic 30.0%
GACATTTAGCTAAAAGGTTT+GGG + chr4_4:55080237-55080256 None:intergenic 30.0%
TGACATTTAGCTAAAAGGTT+TGG + chr4_4:55080238-55080257 None:intergenic 30.0%
CCAATTGACATTTAGCTAAA+AGG + chr4_4:55080243-55080262 None:intergenic 30.0%
!! CCTTTTAGCTAAATGTCAAT+TGG - chr4_4:55080240-55080259 Msa0688820:intron 30.0%
!! ATTGGCTATTTCTGATACAA+AGG - chr4_4:55080258-55080277 Msa0688820:intron 30.0%
! TCCAACTTTGGAAATCAAAT+TGG + chr4_4:55080485-55080504 None:intergenic 30.0%
GCAATAAATCATTCCAACTT+TGG + chr4_4:55080497-55080516 None:intergenic 30.0%
CAGAAGAATAGAGAGAACTT+GGG + chr4_4:55079420-55079439 None:intergenic 35.0%
CTAAGCTTGAAATGAGTCAT+GGG + chr4_4:55079699-55079718 None:intergenic 35.0%
ATGCCTCTTAAAACATTCAC+TGG - chr4_4:55079819-55079838 Msa0688820:CDS 35.0%
!! TGTCCAGTGAATGTTTTAAG+AGG + chr4_4:55079825-55079844 None:intergenic 35.0%
CTGCAGAAAAAACATTGAAC+AGG + chr4_4:55080027-55080046 None:intergenic 35.0%
!! GCTATTTCTGATACAAAGGA+TGG - chr4_4:55080262-55080281 Msa0688820:intron 35.0%
CAGTATACAGGAATTGGTAT+TGG - chr4_4:55080379-55080398 Msa0688820:intron 35.0%
TTGGCAGCATTAGAACTATA+GGG + chr4_4:55080466-55080485 None:intergenic 35.0%
ATTGGCAGCATTAGAACTAT+AGG + chr4_4:55080467-55080486 None:intergenic 35.0%
! GCCAATTTGATTTCCAAAGT+TGG - chr4_4:55080481-55080500 Msa0688820:CDS 35.0%
CAGAAGAATAGAGAGAACTT+GGG + chr4_4:55079420-55079439 None:intergenic 35.0%
CTAAGCTTGAAATGAGTCAT+GGG + chr4_4:55079699-55079718 None:intergenic 35.0%
ATGCCTCTTAAAACATTCAC+TGG - chr4_4:55079819-55079838 Msa0688820:CDS 35.0%
!! TGTCCAGTGAATGTTTTAAG+AGG + chr4_4:55079825-55079844 None:intergenic 35.0%
CTGCAGAAAAAACATTGAAC+AGG + chr4_4:55080027-55080046 None:intergenic 35.0%
!! GCTATTTCTGATACAAAGGA+TGG - chr4_4:55080262-55080281 Msa0688820:intron 35.0%
CAGTATACAGGAATTGGTAT+TGG - chr4_4:55080379-55080398 Msa0688820:intron 35.0%
TTGGCAGCATTAGAACTATA+GGG + chr4_4:55080466-55080485 None:intergenic 35.0%
ATTGGCAGCATTAGAACTAT+AGG + chr4_4:55080467-55080486 None:intergenic 35.0%
! GCCAATTTGATTTCCAAAGT+TGG - chr4_4:55080481-55080500 Msa0688820:CDS 35.0%
CCAGAAGAATAGAGAGAACT+TGG + chr4_4:55079421-55079440 None:intergenic 40.0%
CCAAGTTCTCTCTATTCTTC+TGG - chr4_4:55079418-55079437 Msa0688820:CDS 40.0%
TCACCGTCATCACATTTAAC+TGG + chr4_4:55079483-55079502 None:intergenic 40.0%
GCTAAGCTTGAAATGAGTCA+TGG + chr4_4:55079700-55079719 None:intergenic 40.0%
TTGAGTAATGCACAGATGAC+AGG + chr4_4:55079850-55079869 None:intergenic 40.0%
! TCTCAGTTGTGACCTTTTTC+TGG - chr4_4:55079941-55079960 Msa0688820:intron 40.0%
ACAGGGTAAGTGTAGTGTAA+TGG + chr4_4:55080009-55080028 None:intergenic 40.0%
!! GGTCAGCTATTTTATCAGCA+AGG + chr4_4:55080138-55080157 None:intergenic 40.0%
CAACCATGTACAAATACCAC+AGG - chr4_4:55080169-55080188 Msa0688820:intron 40.0%
TTTGGGTAGTTGGAGAATTG+AGG + chr4_4:55080220-55080239 None:intergenic 40.0%
AGCTAAAAGGTTTGGGTAGT+TGG + chr4_4:55080230-55080249 None:intergenic 40.0%
GAGATTTCGTGAAGTTAGTG+AGG + chr4_4:55080333-55080352 None:intergenic 40.0%
GACACACAGTATACAGGAAT+TGG - chr4_4:55080373-55080392 Msa0688820:intron 40.0%
!! GTATTGGCTCAGAAGATAAC+TGG - chr4_4:55080395-55080414 Msa0688820:intron 40.0%
TGGCAGCATTAGAACTATAG+GGG + chr4_4:55080465-55080484 None:intergenic 40.0%
TGCTCAATTCTCTTGTTAGC+TGG - chr4_4:55080514-55080533 Msa0688820:CDS 40.0%
CCAGAAGAATAGAGAGAACT+TGG + chr4_4:55079421-55079440 None:intergenic 40.0%
CCAAGTTCTCTCTATTCTTC+TGG - chr4_4:55079418-55079437 Msa0688820:CDS 40.0%
TCACCGTCATCACATTTAAC+TGG + chr4_4:55079483-55079502 None:intergenic 40.0%
GCTAAGCTTGAAATGAGTCA+TGG + chr4_4:55079700-55079719 None:intergenic 40.0%
TTGAGTAATGCACAGATGAC+AGG + chr4_4:55079850-55079869 None:intergenic 40.0%
! TCTCAGTTGTGACCTTTTTC+TGG - chr4_4:55079941-55079960 Msa0688820:intron 40.0%
ACAGGGTAAGTGTAGTGTAA+TGG + chr4_4:55080009-55080028 None:intergenic 40.0%
!! GGTCAGCTATTTTATCAGCA+AGG + chr4_4:55080138-55080157 None:intergenic 40.0%
CAACCATGTACAAATACCAC+AGG - chr4_4:55080169-55080188 Msa0688820:intron 40.0%
TTTGGGTAGTTGGAGAATTG+AGG + chr4_4:55080220-55080239 None:intergenic 40.0%
AGCTAAAAGGTTTGGGTAGT+TGG + chr4_4:55080230-55080249 None:intergenic 40.0%
GAGATTTCGTGAAGTTAGTG+AGG + chr4_4:55080333-55080352 None:intergenic 40.0%
GACACACAGTATACAGGAAT+TGG - chr4_4:55080373-55080392 Msa0688820:intron 40.0%
!! GTATTGGCTCAGAAGATAAC+TGG - chr4_4:55080395-55080414 Msa0688820:intron 40.0%
TGGCAGCATTAGAACTATAG+GGG + chr4_4:55080465-55080484 None:intergenic 40.0%
TGCTCAATTCTCTTGTTAGC+TGG - chr4_4:55080514-55080533 Msa0688820:CDS 40.0%
TGTGCATGTGTGCCAGAAAA+AGG + chr4_4:55079956-55079975 None:intergenic 45.0%
GCACCTGTGGTATTTGTACA+TGG + chr4_4:55080175-55080194 None:intergenic 45.0%
TGGTACTGTATTAGCACCTG+TGG + chr4_4:55080188-55080207 None:intergenic 45.0%
ACAGGTGCTAATACAGTACC+AGG - chr4_4:55080187-55080206 Msa0688820:intron 45.0%
GAGAATTGAGGTTCAGTACC+TGG + chr4_4:55080208-55080227 None:intergenic 45.0%
CTCAACGACACACAGTATAC+AGG - chr4_4:55080367-55080386 Msa0688820:intron 45.0%
GGCAGCATTAGAACTATAGG+GGG + chr4_4:55080464-55080483 None:intergenic 45.0%
TGTGCATGTGTGCCAGAAAA+AGG + chr4_4:55079956-55079975 None:intergenic 45.0%
GCACCTGTGGTATTTGTACA+TGG + chr4_4:55080175-55080194 None:intergenic 45.0%
TGGTACTGTATTAGCACCTG+TGG + chr4_4:55080188-55080207 None:intergenic 45.0%
ACAGGTGCTAATACAGTACC+AGG - chr4_4:55080187-55080206 Msa0688820:intron 45.0%
GAGAATTGAGGTTCAGTACC+TGG + chr4_4:55080208-55080227 None:intergenic 45.0%
CTCAACGACACACAGTATAC+AGG - chr4_4:55080367-55080386 Msa0688820:intron 45.0%
GGCAGCATTAGAACTATAGG+GGG + chr4_4:55080464-55080483 None:intergenic 45.0%
TCATCGCAGTCGCGAGATTA+GGG + chr4_4:55079773-55079792 None:intergenic 50.0%
TTCATCGCAGTCGCGAGATT+AGG + chr4_4:55079774-55079793 None:intergenic 50.0%
TCGTGAAGTTAGTGAGGACG+AGG + chr4_4:55080327-55080346 None:intergenic 50.0%
TTGACCACTGACACTCCAAC+AGG - chr4_4:55080430-55080449 Msa0688820:intron 50.0%
! GTTTCCTGTTGGAGTGTCAG+TGG + chr4_4:55080437-55080456 None:intergenic 50.0%
TAGGGGGCAAAGTTTCCTGT+TGG + chr4_4:55080448-55080467 None:intergenic 50.0%
TCATCGCAGTCGCGAGATTA+GGG + chr4_4:55079773-55079792 None:intergenic 50.0%
TTCATCGCAGTCGCGAGATT+AGG + chr4_4:55079774-55079793 None:intergenic 50.0%
TCGTGAAGTTAGTGAGGACG+AGG + chr4_4:55080327-55080346 None:intergenic 50.0%
TTGACCACTGACACTCCAAC+AGG - chr4_4:55080430-55080449 Msa0688820:intron 50.0%
! GTTTCCTGTTGGAGTGTCAG+TGG + chr4_4:55080437-55080456 None:intergenic 50.0%
TAGGGGGCAAAGTTTCCTGT+TGG + chr4_4:55080448-55080467 None:intergenic 50.0%
GTTATGCCTGCCTGTGTTCC+TGG - chr4_4:55080289-55080308 Msa0688820:intron 55.0%
TGAAGCTAGGCCAGGAACAC+AGG + chr4_4:55080302-55080321 None:intergenic 55.0%
TGAGGACGAGGCTTGAAGCT+AGG + chr4_4:55080315-55080334 None:intergenic 55.0%
GTTATGCCTGCCTGTGTTCC+TGG - chr4_4:55080289-55080308 Msa0688820:intron 55.0%
TGAAGCTAGGCCAGGAACAC+AGG + chr4_4:55080302-55080321 None:intergenic 55.0%
TGAGGACGAGGCTTGAAGCT+AGG + chr4_4:55080315-55080334 None:intergenic 55.0%
ACGAGGCTTGAAGCTAGGCC+AGG + chr4_4:55080310-55080329 None:intergenic 60.0%
ACGAGGCTTGAAGCTAGGCC+AGG + chr4_4:55080310-55080329 None:intergenic 60.0%
GCTAGGCCAGGAACACAGGC+AGG + chr4_4:55080298-55080317 None:intergenic 65.0%
GCTAGGCCAGGAACACAGGC+AGG + chr4_4:55080298-55080317 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 55079408 55080555 55079408 ID=Msa0688820;Name=Msa0688820
chr4_4 mRNA 55079408 55080555 55079408 ID=Msa0688820-mRNA-1;Parent=Msa0688820;Name=Msa0688820-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0|0|1|0|0.5|2|0|199
chr4_4 exon 55080465 55080555 55080465 ID=Msa0688820-mRNA-1:exon:14525;Parent=Msa0688820-mRNA-1
chr4_4 exon 55079408 55079916 55079408 ID=Msa0688820-mRNA-1:exon:14524;Parent=Msa0688820-mRNA-1
chr4_4 CDS 55080465 55080555 55080465 ID=Msa0688820-mRNA-1:cds;Parent=Msa0688820-mRNA-1
chr4_4 CDS 55079408 55079916 55079408 ID=Msa0688820-mRNA-1:cds;Parent=Msa0688820-mRNA-1
Gene Sequence

>Msa0688820

ATGGTGTTGCCCAAGTTCTCTCTATTCTTCTGGATTCTTTCTTCTTCATGTGTTATTCTCTTGATGAAATATCCAGTTAAATGTGATGACGATGAGAGAGATAATCTTTATCAGGGTATCAACAAGTATCGAGCATCATTAAACTTGAAATCTCTCACAAAAAATAAAAATGCAGATTGCCTTGCTGATAAAATAGCTGACCAATTCAAGAATCAACCATGTACAAATACCACAGGTGCTAATACAGTACCAGGTACTGAACCTCAATTCTCCAACTACCCAAACCTTTTAGCTAAATGTCAATTGGCTATTTCTGATACAAAGGATGGAGCTGTTATGCCTGCCTGTGTTCCTGGCCTAGCTTCAAGCCTCGTCCTCACTAACTTCACGAAATCTCTCTACTCACAGAATCTCAACGACACACAGTATACAGGAATTGGTATTGGCTCAGAAGATAACTGGATTGTCGTTGTCTTGACCACTGACACTCCAACAGGAAACTTTGCCCCCTATAGTTCTAATGCTGCCAATTTGATTTCCAAAGTTGGAATGATTTATTGCTCAATTCTCTTGTTAGCTGGCAAAATTTTCCTGTTATGA

Protein sequence

>Msa0688820

MVLPKFSLFFWILSSSCVILLMKYPVKCDDDERDNLYQGINKYRASLNLKSLTKNKNADCLADKIADQFKNQPCTNTTGANTVPGTEPQFSNYPNLLAKCQLAISDTKDGAVMPACVPGLASSLVLTNFTKSLYSQNLNDTQYTGIGIGSEDNWIVVVLTTDTPTGNFAPYSSNAANLISKVGMIYCSILLLAGKIFLL*