AlfalfaGEDB | Msa0201100

M. sativa cultivar zhongmu-4 / Msa0201100

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0039200	Msa0201100	0.832414	9.584525e-56	-8.615850e-47
Msa0086820	Msa0201100	0.831888	1.292734e-55	-8.615850e-47
Msa0133670	Msa0201100	0.834336	3.179393e-56	-8.615850e-47
Msa0133880	Msa0201100	0.837400	5.314436e-57	-8.615850e-47
Msa0184120	Msa0201100	0.833552	4.995777e-56	-8.615850e-47
Msa0201100	Msa0243620	0.951995	6.265562e-110	-8.615850e-47
Msa0201100	Msa0243690	0.829606	4.686761e-55	-8.615850e-47
Msa0201100	Msa0256170	0.801174	1.019625e-48	-8.615850e-47
Msa0201100	Msa0282200	0.954221	4.816478e-112	-8.615850e-47
Msa0201100	Msa0306130	0.822062	2.895386e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Find 84 sgRNAs with CRISPR-Local

Find 313 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCAACTTGTTCAAGCAC+AGG	0.588436	2_1:+21865053	None:intergenic
AAAGGCTCACAAGGTCATGC+CGG	0.576546	2_1:-21864878	Msa0201100:exon
AAATGTAAGCATGAATAAAC+TGG	0.406955	2_1:+21863075	None:intergenic
AAATTTAATTGTTACAAAAT+TGG	0.209089	2_1:+21863193	None:intergenic
AATTGATGATACAGGACGTG+AGG	0.664831	2_1:-21864510	Msa0201100:CDS
AATTTGAGAACACGGCTATA+AGG	0.377022	2_1:+21863240	None:intergenic
AATTTGTCGCTTGCCTGGCC+TGG	0.401249	2_1:+21864653	None:intergenic
ACATAACCCCAAACAGATTT+TGG	0.153224	2_1:+21865201	None:intergenic
ACATATCTGATAAATTCGAT+TGG	0.541292	2_1:+21864469	None:intergenic
ACCATACCTTGTGTTCAATA+AGG	0.385103	2_1:+21864426	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGTATGATCCAAAATCTGTT+TGG	-	chr2_1:21863017-21863036	Msa0201100:three_prime_UTR	30.0%
GTATGATCCAAAATCTGTTT+GGG	-	chr2_1:21863018-21863037	Msa0201100:three_prime_UTR	30.0%
TATGATCCAAAATCTGTTTG+GGG	-	chr2_1:21863019-21863038	Msa0201100:three_prime_UTR	30.0%
GTAATTAGCATCCGTAAAAA+AGG	-	chr2_1:21863277-21863296	Msa0201100:three_prime_UTR	30.0%
TGCTGAACAAATTGTACTTA+TGG	-	chr2_1:21863413-21863432	Msa0201100:three_prime_UTR	30.0%
GCTTAAAGAATATGTTTGGT+CGG	+	chr2_1:21863836-21863855	None:intergenic	30.0%
ATACCTTATCCATACAAAAC+CGG	-	chr2_1:21863944-21863963	Msa0201100:intron	30.0%
GGAAATGTGTTTATAAGTGA+TGG	+	chr2_1:21863988-21864007	None:intergenic	30.0%
GTCTCAAATTTGATGAGATA+AGG	+	chr2_1:21864203-21864222	None:intergenic	30.0%
TTAGAATATGACAACGTATG+AGG	-	chr2_1:21864721-21864740	Msa0201100:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	21862945	21865303	21862945	ID=Msa0201100;Name=Msa0201100
chr2_1	mRNA	21862945	21865303	21862945	ID=Msa0201100-mRNA-1;Parent=Msa0201100;Name=Msa0201100-mRNA-1;_AED=0.04;_eAED=0.04;_QI=219\|1\|1\|1\|1\|0.8\|5\|693\|156
chr2_1	exon	21862945	21863494	21862945	ID=Msa0201100-mRNA-1:exon:8790;Parent=Msa0201100-mRNA-1
chr2_1	exon	21863599	21863760	21863599	ID=Msa0201100-mRNA-1:exon:8789;Parent=Msa0201100-mRNA-1
chr2_1	exon	21864433	21864909	21864433	ID=Msa0201100-mRNA-1:exon:8788;Parent=Msa0201100-mRNA-1
chr2_1	exon	21865007	21865093	21865007	ID=Msa0201100-mRNA-1:exon:8787;Parent=Msa0201100-mRNA-1
chr2_1	exon	21865197	21865303	21865197	ID=Msa0201100-mRNA-1:exon:8786;Parent=Msa0201100-mRNA-1
chr2_1	five_prime_UTR	21865197	21865303	21865197	ID=Msa0201100-mRNA-1:five_prime_utr;Parent=Msa0201100-mRNA-1
chr2_1	five_prime_UTR	21865007	21865093	21865007	ID=Msa0201100-mRNA-1:five_prime_utr;Parent=Msa0201100-mRNA-1
chr2_1	five_prime_UTR	21864885	21864909	21864885	ID=Msa0201100-mRNA-1:five_prime_utr;Parent=Msa0201100-mRNA-1
chr2_1	CDS	21864433	21864884	21864433	ID=Msa0201100-mRNA-1:cds;Parent=Msa0201100-mRNA-1
chr2_1	CDS	21863742	21863760	21863742	ID=Msa0201100-mRNA-1:cds;Parent=Msa0201100-mRNA-1
chr2_1	three_prime_UTR	21863599	21863741	21863599	ID=Msa0201100-mRNA-1:three_prime_utr;Parent=Msa0201100-mRNA-1
chr2_1	three_prime_UTR	21862945	21863494	21862945	ID=Msa0201100-mRNA-1:three_prime_utr;Parent=Msa0201100-mRNA-1

Gene Sequence

>Msa0201100

ATGCCGGAACTGTTCCAATGTCCATGCGTCAAGATCCTATGGTGGCTGCTGCTGAACAAATTGTACTTATGGAAAGCCTCTGCAAACATCCTGAAGAGTACCTTTCTTACGATGGTCGTTGCAGTGATTCATCGATTAAATCACTTTCAAGTTCACTTGTTTGTACTGTTGGTGAAATATCAACATGGCCAAGTGCTAGTAATGTCATTCCAGGCCAGGCAAGCGACAAATTTCCAAAAAGCTGTTGATCTGGTGATCTTCAAGTGGATATGTTTTTTGCTTTCTCAAATTGAAGCTTGTGATGATAATGTGATGATTGGTTGCAGGTTACATATACCGTGGATATACGAGCAATTGATGATACAGGACGTGAGGCTGTTATCTATGATTTATCCAATCGAATTTATCAGATATGTGACAAGCGTTCAGTTTCCTGCCTTATTGAACACAAGCATGATGCAGGTGCTGTGA

Protein sequence

>Msa0201100

MPELFQCPCVKILWWLLLNKLYLWKASANILKSTFLTMVVAVIHRLNHFQVHLFVLLVKYQHGQVLVMSFQARQATNFQKAVDLVIFKWICFLLSQIEACDDNVMIGCRLHIPWIYEQLMIQDVRLLSMIYPIEFIRYVTSVQFPALLNTSMMQVL*