AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1343310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 108 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAGCAACCTTATCATTTC+TGG 0.089860 tig0006360:+2664 Msa1343310:three_prime_UTR
ACATAACCCCAAACAGATTT+TGG 0.153224 tig0006360:-871 None:intergenic
AAATTTAATTGTTACAAAAT+TGG 0.209089 tig0006360:-2857 Msa1343320:intron
TATTTGTAGCATGTAATTAT+AGG 0.239260 tig0006360:+2730 Msa1343310:three_prime_UTR
TTTCCTTGATACCTCTTATC+AGG 0.279914 tig0006360:-705 None:intergenic
TTCCCACTTGGTGTCGTTAA+AGG 0.289921 tig0006360:+1040 Msa1343310:five_prime_UTR
CTTATGCTGCAAGCTGTAAT+AGG 0.294960 tig0006360:+3022 Msa1343310:three_prime_UTR
CTCTTGTATGTGTATTCATT+TGG 0.295573 tig0006360:+448 Msa1343310:five_prime_UTR
GTATGATCCAAAATCTGTTT+GGG 0.312638 tig0006360:+864 Msa1343310:five_prime_UTR
TTTGTGTAGGTCACGGTTAA+AGG 0.312855 tig0006360:+1174 Msa1343310:intron
TCATGTGATCTAATCTTTGC+AGG 0.320706 tig0006360:+584 Msa1343310:intron
TTCAGCTGTGAACTTAGTTC+TGG 0.333589 tig0006360:-2361 None:intergenic
TGCTGAACAAATTGTACTTA+TGG 0.340654 tig0006360:+1257 Msa1343310:CDS
AGTATGATCCAAAATCTGTT+TGG 0.358696 tig0006360:+863 Msa1343310:five_prime_UTR
GTTGATGCTGGTAAATTTGA+TGG 0.363766 tig0006360:+204 Msa1343310:five_prime_UTR
AATTTGAGAACACGGCTATA+AGG 0.377022 tig0006360:-2810 Msa1343320:intron
AATTCATCGCTTGCCTGGCC+TGG 0.383667 tig0006360:-1417 None:intergenic
ACCATACCTTGTGTTCAATA+AGG 0.385103 tig0006360:-1644 None:intergenic
CTCTGCAATATCTGCATTGA+AGG 0.404600 tig0006360:+242 Msa1343310:five_prime_UTR
AAATGTAAGCATGAATAAAC+TGG 0.406955 tig0006360:-2975 Msa1343320:intron
TTGGAAATTCATCGCTTGCC+TGG 0.418673 tig0006360:-1422 None:intergenic
ATGGAATGCTGCAAAATCTA+AGG 0.421423 tig0006360:+277 Msa1343310:five_prime_UTR
TCGTAAGAAAGGTACTCTTC+AGG 0.428537 tig0006360:-1297 None:intergenic
CTTGACACACTAACCTCAAC+AGG 0.432855 tig0006360:-303 None:intergenic
AGTTCACTTGTTTGTACTGT+TGG 0.437924 tig0006360:+1357 Msa1343310:CDS
GAACAAGTTGGTTTCCCACT+TGG 0.439155 tig0006360:+1028 Msa1343310:five_prime_UTR
TTGGTTGGCCTCCTCTTGAT+AGG 0.440619 tig0006360:-2838 Msa1343320:intron
ATACGAGCAATTGATGATCT+TGG 0.445837 tig0006360:+1552 Msa1343310:CDS
GGTGTCGTTAAAGGCATAGC+TGG 0.445854 tig0006360:+1049 Msa1343310:five_prime_UTR
CTAGGCAGTGGGGCTATAGC+TGG 0.446972 tig0006360:+654 Msa1343310:five_prime_UTR
AGTGCTAGTAATGTCATTCC+AGG 0.447467 tig0006360:+1399 Msa1343310:CDS
ATAATGTGATGATTGGTTGC+AGG 0.450358 tig0006360:+1512 Msa1343310:CDS
AGATTTCAAACTACTTTCCT+AGG 0.450999 tig0006360:+636 Msa1343310:five_prime_UTR
CATGGACATTGGAACAGTTC+CGG 0.452698 tig0006360:-1211 None:intergenic
ATGTGCTAAACGATGATGTA+TGG 0.472847 tig0006360:+2633 Msa1343310:three_prime_UTR
AAACTACTTTCCTAGGCAGT+GGG 0.473932 tig0006360:+643 Msa1343310:five_prime_UTR
AACGATGATGTATGGGCGGC+AGG 0.475444 tig0006360:+2641 Msa1343310:three_prime_UTR
TTTGATCATATGTATGATCA+TGG 0.480276 tig0006360:-2911 Msa1343320:intron
CCAACATTAATGAGTGGAGC+AGG 0.482594 tig0006360:+2439 Msa1343310:three_prime_UTR
TGTGATGATAATGTGATGAT+TGG 0.483174 tig0006360:+1505 Msa1343310:CDS
CAGCTATAGCCCCACTGCCT+AGG 0.492749 tig0006360:-653 None:intergenic
CATTGGAGATACCTGATAAG+AGG 0.498225 tig0006360:+694 Msa1343310:five_prime_UTR
TTAATTGTTACAAAATTGGT+TGG 0.498310 tig0006360:-2853 Msa1343320:intron
CAAACTACTTTCCTAGGCAG+TGG 0.498481 tig0006360:+642 Msa1343310:five_prime_UTR
AGGTTAGTGTGTCAAGCAAC+TGG 0.499301 tig0006360:+310 Msa1343310:five_prime_UTR
GTCCATGCGTCAAGATCCTA+TGG 0.502369 tig0006360:+1227 Msa1343310:CDS
TAGGACACTGTTGTTGATGC+TGG 0.515212 tig0006360:+192 Msa1343310:intron
GCTGCAGCTTCACATTGAGC+AGG 0.516545 tig0006360:+994 Msa1343310:intron
GTACTTGATAAATTGAAGAA+AGG 0.516995 tig0006360:+2943 Msa1343310:three_prime_UTR
GGTATCTCCAATGTTGTAGC+AGG 0.519067 tig0006360:-684 None:intergenic
TACCTTGTGTTCAATAAGGC+AGG 0.520522 tig0006360:-1640 None:intergenic
TGCAACGACCATCGTAAGAA+AGG 0.520984 tig0006360:-1308 None:intergenic
GGTAAATTTGATGGAGCACT+AGG 0.522882 tig0006360:+213 Msa1343310:five_prime_UTR
GGATCTTGACGCATGGACAT+TGG 0.526811 tig0006360:-1222 None:intergenic
TTGAAGGTTATGAATGTCAA+TGG 0.529056 tig0006360:+258 Msa1343310:five_prime_UTR
TGTTCACCTCAGCATGATGC+AGG 0.530008 tig0006360:+2322 Msa1343310:intron
AGCTGGGCAGACAAGATTGA+AGG 0.535514 tig0006360:+1066 Msa1343310:five_prime_UTR
TAGTGTGTCAAGCAACTGGC+TGG 0.540823 tig0006360:+314 Msa1343310:five_prime_UTR
ACATATCTGATAAATTCGAT+TGG 0.541292 tig0006360:-1601 None:intergenic
TAATGATATGGTCTGATGCA+CGG 0.544889 tig0006360:+355 Msa1343310:five_prime_UTR
GGTCCTGTGCTTGAACAAGT+TGG 0.545766 tig0006360:+1016 Msa1343310:five_prime_UTR
TGGTGAATTGGGATTCAAGA+TGG 0.549402 tig0006360:-480 None:intergenic
TAGTAATGTCATTCCAGGCC+AGG 0.550343 tig0006360:+1404 Msa1343310:CDS
AAATCTAAGGCGTCCTGTTG+AGG 0.552501 tig0006360:+290 Msa1343310:five_prime_UTR
TCGTTTAGCACATCCTCTTG+TGG 0.566941 tig0006360:-2623 Msa1343320:CDS
GTATATGTGAACATTGTAAG+TGG 0.568506 tig0006360:+3116 Msa1343310:three_prime_UTR
CTGCAGCTTCACATTGAGCA+GGG 0.575143 tig0006360:+995 Msa1343310:intron
TTCCTGCCTTATTGAACACA+AGG 0.575456 tig0006360:+1638 Msa1343310:CDS
CAAATTATTCTCCTATCAAG+AGG 0.575584 tig0006360:+2827 Msa1343310:three_prime_UTR
ATCTGTTTGGGGTTATGTCG+AGG 0.575812 tig0006360:+876 Msa1343310:five_prime_UTR
AAAGGCTCACAAGGTCATGC+CGG 0.576546 tig0006360:+1192 Msa1343310:exon
CAGCTTATTCTGCACTCAAG+AGG 0.578224 tig0006360:+2389 Msa1343310:three_prime_UTR
TGTTCAGCAGCAGCCACCAT+AGG 0.579164 tig0006360:-1243 None:intergenic
TGGAGCAGGGCATGATGCCA+TGG 0.582009 tig0006360:+2453 Msa1343310:three_prime_UTR
AAACCAACTTGTTCAAGCAC+AGG 0.588436 tig0006360:-1019 None:intergenic
ATCAATTGCTCGTATATCCA+CGG 0.588827 tig0006360:-1544 None:intergenic
CCTGCTCCACTCATTAATGT+TGG 0.589437 tig0006360:-2439 None:intergenic
GGAATGACATTACTAGCACT+TGG 0.589744 tig0006360:-1396 None:intergenic
TTCTGCACTCAAGAGGATGG+AGG 0.594618 tig0006360:+2396 Msa1343310:three_prime_UTR
AGAGAACTCGATAGAAGCTG+TGG 0.605459 tig0006360:+819 Msa1343310:five_prime_UTR
GGCAATGTCTCACTTAACAA+AGG 0.607328 tig0006360:+2474 Msa1343310:three_prime_UTR
ACTCTTCAGGATGTTTGCAG+AGG 0.608033 tig0006360:-1284 None:intergenic
TTATTCTGCACTCAAGAGGA+TGG 0.613099 tig0006360:+2393 Msa1343310:three_prime_UTR
GTGTCGTTAAAGGCATAGCT+GGG 0.619543 tig0006360:+1050 Msa1343310:five_prime_UTR
TCTGCACTCAAGAGGATGGA+GGG 0.619874 tig0006360:+2397 Msa1343310:three_prime_UTR
ATGCTGTTTGTGCGATGTCG+CGG 0.620832 tig0006360:+2584 Msa1343310:three_prime_UTR
GAACTCGATAGAAGCTGTGG+AGG 0.623059 tig0006360:+822 Msa1343310:five_prime_UTR
ATGCCTTTAACGACACCAAG+TGG 0.623755 tig0006360:-1043 None:intergenic
TTGATCTGGTGATCTTCAAG+TGG 0.624881 tig0006360:+1452 Msa1343310:CDS
CATAAGTCACTCTCCACAAG+AGG 0.625552 tig0006360:+2610 Msa1343310:three_prime_UTR
CACCATAGGATCTTGACGCA+TGG 0.629370 tig0006360:-1229 None:intergenic
GTCACGGTTAAAGGCTCACA+AGG 0.633455 tig0006360:+1183 Msa1343310:five_prime_UTR
AGGAGAATAATTTGAGAACA+CGG 0.640264 tig0006360:-2818 Msa1343320:intron
TGTGCTAAACGATGATGTAT+GGG 0.641420 tig0006360:+2634 Msa1343310:three_prime_UTR
GAAGTACCAACATTAATGAG+TGG 0.642265 tig0006360:+2433 Msa1343310:three_prime_UTR
GTTGCAGGTTACATATACCG+TGG 0.655260 tig0006360:+1527 Msa1343310:CDS
TATGATCCAAAATCTGTTTG+GGG 0.656816 tig0006360:+865 Msa1343310:five_prime_UTR
TGTTAAGTGAGACATTGCCA+TGG 0.658188 tig0006360:-2470 None:intergenic
CAACATTAATGAGTGGAGCA+GGG 0.661114 tig0006360:+2440 Msa1343310:three_prime_UTR
ATTATTCTCCTATCAAGAGG+AGG 0.665582 tig0006360:+2830 Msa1343310:three_prime_UTR
TGCCTTTAACGACACCAAGT+GGG 0.666563 tig0006360:-1042 None:intergenic
GAAGAGTACCTTTCTTACGA+TGG 0.667691 tig0006360:+1300 Msa1343310:CDS
CTGTTTGTGCGATGTCGCGG+AGG 0.676245 tig0006360:+2587 Msa1343310:three_prime_UTR
AACTACTTTCCTAGGCAGTG+GGG 0.682431 tig0006360:+644 Msa1343310:five_prime_UTR
AATTGATGATCTTGGACGTG+AGG 0.685467 tig0006360:+1560 Msa1343310:CDS
CTGTTGGTGAAATATCAACA+TGG 0.708340 tig0006360:+1373 Msa1343310:CDS
CATGCGTCAAGATCCTATGG+TGG 0.726797 tig0006360:+1230 Msa1343310:CDS
GCTAAACGATGATGTATGGG+CGG 0.742622 tig0006360:+2637 Msa1343310:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATAAAATTCTTAGAATTT+AGG + tig0006360:1763-1782 Msa1343310:intron 10.0%
!! TATAAAATTCTTAGAATTTA+GGG + tig0006360:1764-1783 Msa1343310:intron 10.0%
!! TATAAATACTTATAGTAAAT+AGG + tig0006360:2177-2196 Msa1343310:intron 10.0%
!! ATAATCAAATGTTAATATAT+TGG + tig0006360:2514-2533 Msa1343310:intron 10.0%
!! AAATTTAATTGTTACAAAAT+TGG - tig0006360:2860-2879 Msa1343320:intron 10.0%
!!! TTGCAATATTTTTAATGATA+TGG + tig0006360:343-362 Msa1343310:five_prime_UTR 15.0%
!!! TTTATTTATAAACGTTTTTC+AGG + tig0006360:770-789 Msa1343310:intron 15.0%
!!! AACATGAAGTTTTTTTTTAT+GGG + tig0006360:3085-3104 Msa1343310:three_prime_UTR 15.0%
!!! GTTAATTGTAAATTTTAGTG+AGG + tig0006360:126-145 Msa1343310:intron 20.0%
!! AAACAGCATATCATTAATAT+CGG - tig0006360:1156-1175 None:intergenic 20.0%
!! TTATGCTTAAAGAATATGTT+TGG - tig0006360:1684-1703 None:intergenic 20.0%
!!! AGTAAATAGGTATTTTTTGT+TGG + tig0006360:2190-2209 Msa1343310:intron 20.0%
!! TATTTGTAGCATGTAATTAT+AGG + tig0006360:2730-2749 Msa1343310:three_prime_UTR 20.0%
!! TTAATTGTTACAAAATTGGT+TGG - tig0006360:2856-2875 Msa1343320:intron 20.0%
!!! CAACATGAAGTTTTTTTTTA+TGG + tig0006360:3084-3103 Msa1343310:three_prime_UTR 20.0%
! GTTTCATTCAAAGTTAATTG+AGG - tig0006360:89-108 None:intergenic 25.0%
! TACAATTAACTGTCAAGAAA+TGG - tig0006360:116-135 None:intergenic 25.0%
!!! GATGGATACTTATTTTTGTT+AGG + tig0006360:173-192 Msa1343310:intron 25.0%
! ATGATTCATTGAAATGAATC+GGG - tig0006360:514-533 None:intergenic 25.0%
! GATGATTCATTGAAATGAAT+CGG - tig0006360:515-534 None:intergenic 25.0%
! ATTTCAATGAATCATCATCT+TGG + tig0006360:519-538 Msa1343310:intron 25.0%
! ACATATCTGATAAATTCGAT+TGG - tig0006360:1604-1623 None:intergenic 25.0%
! GAAATGTGTTTATAAGTGAT+GGG - tig0006360:1831-1850 None:intergenic 25.0%
! AAATGTGTTTAGATAAGTAG+TGG - tig0006360:2020-2039 None:intergenic 25.0%
! TACTTATCTAAACACATTTC+AGG + tig0006360:2021-2040 Msa1343310:intron 25.0%
! ACTCTTAATAAAAACGAATG+TGG + tig0006360:2065-2084 Msa1343310:intron 25.0%
!! TGTTTTATCCTTTTAGATTG+AGG - tig0006360:2114-2133 None:intergenic 25.0%
!!! AAATAGGTATTTTTTGTTGG+TGG + tig0006360:2193-2212 Msa1343310:intron 25.0%
!!! AATAGGTATTTTTTGTTGGT+GGG + tig0006360:2194-2213 Msa1343310:intron 25.0%
! TTAGAATATGACAATGAATG+AGG + tig0006360:2545-2564 Msa1343310:intron 25.0%
!! TAGATTTTCCAGAAATGATA+AGG - tig0006360:2675-2694 Msa1343320:intron 25.0%
! TTTGATCATATGTATGATCA+TGG - tig0006360:2914-2933 Msa1343320:intron 25.0%
! GTACTTGATAAATTGAAGAA+AGG + tig0006360:2943-2962 Msa1343310:three_prime_UTR 25.0%
! AAATGTAAGCATGAATAAAC+TGG - tig0006360:2978-2997 Msa1343320:intron 25.0%
TTGAAGGTTATGAATGTCAA+TGG + tig0006360:258-277 Msa1343310:five_prime_UTR 30.0%
CTCTTGTATGTGTATTCATT+TGG + tig0006360:448-467 Msa1343310:five_prime_UTR 30.0%
!!! GAATGATGCCACTTTTTTAT+TGG + tig0006360:550-569 Msa1343310:intron 30.0%
AGATGACACCAATAAAAAAG+TGG - tig0006360:561-580 None:intergenic 30.0%
! ATTGCATTTTGTGATGAAGA+GGG + tig0006360:609-628 Msa1343310:five_prime_UTR 30.0%
AGATTTCAAACTACTTTCCT+AGG + tig0006360:636-655 Msa1343310:five_prime_UTR 30.0%
! TATTTTACCTGCTACAACAT+TGG + tig0006360:677-696 Msa1343310:five_prime_UTR 30.0%
!!! AACGTTTTTCAGGAATGTAA+CGG + tig0006360:780-799 Msa1343310:intron 30.0%
AGTATGATCCAAAATCTGTT+TGG + tig0006360:863-882 Msa1343310:five_prime_UTR 30.0%
GTATGATCCAAAATCTGTTT+GGG + tig0006360:864-883 Msa1343310:five_prime_UTR 30.0%
TATGATCCAAAATCTGTTTG+GGG + tig0006360:865-884 Msa1343310:five_prime_UTR 30.0%
ACTATAAACGACTAATGCAA+AGG - tig0006360:911-930 None:intergenic 30.0%
TTTGCATTAGTCGTTTATAG+TGG + tig0006360:910-929 Msa1343310:intron 30.0%
AGTAATTAGCATCCGAAAAA+AGG + tig0006360:1121-1140 Msa1343310:intron 30.0%
!!! AATGATATGCTGTTTTGTGT+AGG + tig0006360:1161-1180 Msa1343310:intron 30.0%
TGCTGAACAAATTGTACTTA+TGG + tig0006360:1257-1276 Msa1343310:CDS 30.0%
! TGTGATGATAATGTGATGAT+TGG + tig0006360:1505-1524 Msa1343310:CDS 30.0%
GCTTAAAGAATATGTTTGGT+CGG - tig0006360:1680-1699 None:intergenic 30.0%
ATACCTTATCCATACAAAAC+CGG + tig0006360:1788-1807 Msa1343310:intron 30.0%
GGAAATGTGTTTATAAGTGA+TGG - tig0006360:1832-1851 None:intergenic 30.0%
! GTTTATCCAAGACACATTTT+AGG - tig0006360:1926-1945 None:intergenic 30.0%
GTCTCAAATTTGATGAGATA+AGG - tig0006360:2047-2066 None:intergenic 30.0%
!!! GTTTGTTTTAGATCTTGTCT+AGG + tig0006360:2251-2270 Msa1343310:intron 30.0%
!! AGGTTTTTCAGTTTGTTTTC+TGG + tig0006360:2271-2290 Msa1343310:intron 30.0%
TTTAGCAACCTTATCATTTC+TGG + tig0006360:2664-2683 Msa1343310:three_prime_UTR 30.0%
AGGAGAATAATTTGAGAACA+CGG - tig0006360:2821-2840 Msa1343320:intron 30.0%
CAAATTATTCTCCTATCAAG+AGG + tig0006360:2827-2846 Msa1343310:three_prime_UTR 30.0%
GTATATGTGAACATTGTAAG+TGG + tig0006360:3116-3135 Msa1343310:three_prime_UTR 30.0%
! TTTCATGTCACTGACTATGA+TGG + tig0006360:155-174 Msa1343310:intron 35.0%
GTTGATGCTGGTAAATTTGA+TGG + tig0006360:204-223 Msa1343310:five_prime_UTR 35.0%
ATGGAATGCTGCAAAATCTA+AGG + tig0006360:277-296 Msa1343310:five_prime_UTR 35.0%
TAATGATATGGTCTGATGCA+CGG + tig0006360:355-374 Msa1343310:five_prime_UTR 35.0%
AATCATCATCTTGGAAATGC+TGG + tig0006360:528-547 Msa1343310:intron 35.0%
TCATGTGATCTAATCTTTGC+AGG + tig0006360:584-603 Msa1343310:intron 35.0%
! GATTGCATTTTGTGATGAAG+AGG + tig0006360:608-627 Msa1343310:five_prime_UTR 35.0%
!! ATACCTGATAAGAGGTATCA+AGG + tig0006360:702-721 Msa1343310:intron 35.0%
TTTCCTTGATACCTCTTATC+AGG - tig0006360:708-727 None:intergenic 35.0%
! ACATAACCCCAAACAGATTT+TGG - tig0006360:874-893 None:intergenic 35.0%
TAGTCGTTTATAGTGGTGTT+TGG + tig0006360:917-936 Msa1343310:intron 35.0%
!!! CGGATTTCAGTTCCTTTTTT+CGG - tig0006360:1136-1155 None:intergenic 35.0%
AGTTCACTTGTTTGTACTGT+TGG + tig0006360:1357-1376 Msa1343310:CDS 35.0%
! CTGTTGGTGAAATATCAACA+TGG + tig0006360:1373-1392 Msa1343310:CDS 35.0%
TTTCCAAAAAGCTGTTGATC+TGG + tig0006360:1438-1457 Msa1343310:CDS 35.0%
! ATAATGTGATGATTGGTTGC+AGG + tig0006360:1512-1531 Msa1343310:CDS 35.0%
ATCAATTGCTCGTATATCCA+CGG - tig0006360:1547-1566 None:intergenic 35.0%
! ATACGAGCAATTGATGATCT+TGG + tig0006360:1552-1571 Msa1343310:CDS 35.0%
ACCATACCTTGTGTTCAATA+AGG - tig0006360:1647-1666 None:intergenic 35.0%
TGATGTCTTTCTAAGTGAGA+GGG + tig0006360:1718-1737 Msa1343310:intron 35.0%
!! AAACCGGTTTTGTATGGATA+AGG - tig0006360:1794-1813 None:intergenic 35.0%
GTCTCACGTAGTATGAAATA+GGG - tig0006360:1858-1877 None:intergenic 35.0%
AGTCTCACGTAGTATGAAAT+AGG - tig0006360:1859-1878 None:intergenic 35.0%
!! CCAGTTTTGTAGAGATTAGT+TGG - tig0006360:1978-1997 None:intergenic 35.0%
CCAACTAATCTCTACAAAAC+TGG + tig0006360:1975-1994 Msa1343310:intron 35.0%
! ACAAGATCTAAAACAAACCC+AGG - tig0006360:2249-2268 None:intergenic 35.0%
GGTGAACACAGAAAAATCAT+TGG - tig0006360:2310-2329 None:intergenic 35.0%
! GAAGTACCAACATTAATGAG+TGG + tig0006360:2433-2452 Msa1343310:three_prime_UTR 35.0%
CAATGAATGAGGATTTGAAC+AGG + tig0006360:2556-2575 Msa1343310:intron 35.0%
ATGTGCTAAACGATGATGTA+TGG + tig0006360:2633-2652 Msa1343310:three_prime_UTR 35.0%
TGTGCTAAACGATGATGTAT+GGG + tig0006360:2634-2653 Msa1343310:three_prime_UTR 35.0%
AATTTGAGAACACGGCTATA+AGG - tig0006360:2813-2832 Msa1343320:intron 35.0%
ATTATTCTCCTATCAAGAGG+AGG + tig0006360:2830-2849 Msa1343310:three_prime_UTR 35.0%
! TGCAAATGCAGAAGCTTTGT+TGG + tig0006360:29-48 Msa1343310:five_prime_UTR 40.0%
!! AATGCAGAAGCTTTGTTGGT+TGG + tig0006360:33-52 Msa1343310:five_prime_UTR 40.0%
! TTTGTTGGTTGGATCTCACA+TGG + tig0006360:44-63 Msa1343310:five_prime_UTR 40.0%
! GGTAAATTTGATGGAGCACT+AGG + tig0006360:213-232 Msa1343310:five_prime_UTR 40.0%
CTCTGCAATATCTGCATTGA+AGG + tig0006360:242-261 Msa1343310:five_prime_UTR 40.0%
TGGTGAATTGGGATTCAAGA+TGG - tig0006360:483-502 None:intergenic 40.0%
AAATGAATCGGGATTCAGCA+TGG - tig0006360:503-522 None:intergenic 40.0%
AAACTACTTTCCTAGGCAGT+GGG + tig0006360:643-662 Msa1343310:five_prime_UTR 40.0%
CATTGGAGATACCTGATAAG+AGG + tig0006360:694-713 Msa1343310:five_prime_UTR 40.0%
AGCTGCAGCACATAAGAAAT+GGG - tig0006360:983-1002 None:intergenic 40.0%
AAGCTGCAGCACATAAGAAA+TGG - tig0006360:984-1003 None:intergenic 40.0%
! AAACCAACTTGTTCAAGCAC+AGG - tig0006360:1022-1041 None:intergenic 40.0%
!!! ATGCTGTTTTGTGTAGGTCA+CGG + tig0006360:1167-1186 Msa1343310:intron 40.0%
TTTGTGTAGGTCACGGTTAA+AGG + tig0006360:1174-1193 Msa1343310:intron 40.0%
!! TCGTAAGAAAGGTACTCTTC+AGG - tig0006360:1300-1319 None:intergenic 40.0%
!! GAAGAGTACCTTTCTTACGA+TGG + tig0006360:1300-1319 Msa1343310:CDS 40.0%
GGAATGACATTACTAGCACT+TGG - tig0006360:1399-1418 None:intergenic 40.0%
AGTGCTAGTAATGTCATTCC+AGG + tig0006360:1399-1418 Msa1343310:CDS 40.0%
! TCACCAGATCAACAGCTTTT+TGG - tig0006360:1444-1463 None:intergenic 40.0%
TTGATCTGGTGATCTTCAAG+TGG + tig0006360:1452-1471 Msa1343310:CDS 40.0%
! AATTGATGATCTTGGACGTG+AGG + tig0006360:1560-1579 Msa1343310:CDS 40.0%
! TTCCTGCCTTATTGAACACA+AGG + tig0006360:1638-1657 Msa1343310:CDS 40.0%
TACCTTGTGTTCAATAAGGC+AGG - tig0006360:1643-1662 None:intergenic 40.0%
! GCCTTATTGAACACAAGGTA+TGG + tig0006360:1643-1662 Msa1343310:intron 40.0%
CTGATGTCTTTCTAAGTGAG+AGG + tig0006360:1717-1736 Msa1343310:intron 40.0%
!! CCTTACAAACCGGTTTTGTA+TGG - tig0006360:1800-1819 None:intergenic 40.0%
!! CCATACAAAACCGGTTTGTA+AGG + tig0006360:1797-1816 Msa1343310:intron 40.0%
ACGTAGTATGAAATAGGGAC+TGG - tig0006360:1853-1872 None:intergenic 40.0%
ACTGTTCTCACATTCAGAAC+TGG + tig0006360:1887-1906 Msa1343310:intron 40.0%
!! CCAAGACACATTTTAGGTCT+TGG - tig0006360:1920-1939 None:intergenic 40.0%
CCAAGACCTAAAATGTGTCT+TGG + tig0006360:1917-1936 Msa1343310:intron 40.0%
!! CTCTACAAAACTGGCTTGTA+AGG + tig0006360:1984-2003 Msa1343310:intron 40.0%
AGGTCGAACCTCAATCTAAA+AGG + tig0006360:2103-2122 Msa1343310:intron 40.0%
ACACTACTTACCACTATGCT+AGG + tig0006360:2134-2153 Msa1343310:intron 40.0%
CTAAAACAAACCCAGGCATA+AGG - tig0006360:2242-2261 None:intergenic 40.0%
TTCAGCTGTGAACTTAGTTC+TGG - tig0006360:2364-2383 None:intergenic 40.0%
TTATTCTGCACTCAAGAGGA+TGG + tig0006360:2393-2412 Msa1343310:three_prime_UTR 40.0%
CAACATTAATGAGTGGAGCA+GGG + tig0006360:2440-2459 Msa1343310:three_prime_UTR 40.0%
TGTTAAGTGAGACATTGCCA+TGG - tig0006360:2473-2492 None:intergenic 40.0%
GGCAATGTCTCACTTAACAA+AGG + tig0006360:2474-2493 Msa1343310:three_prime_UTR 40.0%
TGAATGAGGATTTGAACAGG+TGG + tig0006360:2559-2578 Msa1343310:intron 40.0%
GAATGAGGATTTGAACAGGT+GGG + tig0006360:2560-2579 Msa1343310:intron 40.0%
CTTATGCTGCAAGCTGTAAT+AGG + tig0006360:3022-3041 Msa1343310:three_prime_UTR 40.0%
TAGGACACTGTTGTTGATGC+TGG + tig0006360:192-211 Msa1343310:intron 45.0%
! AAATCTAAGGCGTCCTGTTG+AGG + tig0006360:290-309 Msa1343310:five_prime_UTR 45.0%
CTTGACACACTAACCTCAAC+AGG - tig0006360:306-325 None:intergenic 45.0%
AGGTTAGTGTGTCAAGCAAC+TGG + tig0006360:310-329 Msa1343310:five_prime_UTR 45.0%
GGGATTCAGCATGGTGAATT+GGG - tig0006360:494-513 None:intergenic 45.0%
CAAACTACTTTCCTAGGCAG+TGG + tig0006360:642-661 Msa1343310:five_prime_UTR 45.0%
AACTACTTTCCTAGGCAGTG+GGG + tig0006360:644-663 Msa1343310:five_prime_UTR 45.0%
GGTATCTCCAATGTTGTAGC+AGG - tig0006360:687-706 None:intergenic 45.0%
AGAGAACTCGATAGAAGCTG+TGG + tig0006360:819-838 Msa1343310:five_prime_UTR 45.0%
! ATCTGTTTGGGGTTATGTCG+AGG + tig0006360:876-895 Msa1343310:five_prime_UTR 45.0%
!! GAACAAGTTGGTTTCCCACT+TGG + tig0006360:1028-1047 Msa1343310:five_prime_UTR 45.0%
!! TTCCCACTTGGTGTCGTTAA+AGG + tig0006360:1040-1059 Msa1343310:five_prime_UTR 45.0%
TGCCTTTAACGACACCAAGT+GGG - tig0006360:1045-1064 None:intergenic 45.0%
ATGCCTTTAACGACACCAAG+TGG - tig0006360:1046-1065 None:intergenic 45.0%
! GTGTCGTTAAAGGCATAGCT+GGG + tig0006360:1050-1069 Msa1343310:five_prime_UTR 45.0%
CATGGACATTGGAACAGTTC+CGG - tig0006360:1214-1233 None:intergenic 45.0%
ACTCTTCAGGATGTTTGCAG+AGG - tig0006360:1287-1306 None:intergenic 45.0%
TGCAACGACCATCGTAAGAA+AGG - tig0006360:1311-1330 None:intergenic 45.0%
TAGTAATGTCATTCCAGGCC+AGG + tig0006360:1404-1423 Msa1343310:CDS 45.0%
TTGGAAATTCATCGCTTGCC+TGG - tig0006360:1425-1444 None:intergenic 45.0%
GTTGCAGGTTACATATACCG+TGG + tig0006360:1527-1546 Msa1343310:CDS 45.0%
GAGGGAGTAACAGAAAAGTC+AGG + tig0006360:1736-1755 Msa1343310:intron 45.0%
AACTAGCATGCCTAGCATAG+TGG - tig0006360:2147-2166 None:intergenic 45.0%
! TTGTTGGTGGGCCTAAAGTA+AGG + tig0006360:2206-2225 Msa1343310:intron 45.0%
TGGGCCTAAAGTAAGGACTT+GGG + tig0006360:2213-2232 Msa1343310:intron 45.0%
AAACAAACCCAGGCATAAGG+TGG - tig0006360:2239-2258 None:intergenic 45.0%
CAGCTTATTCTGCACTCAAG+AGG + tig0006360:2389-2408 Msa1343310:three_prime_UTR 45.0%
CCTGCTCCACTCATTAATGT+TGG - tig0006360:2442-2461 None:intergenic 45.0%
CCAACATTAATGAGTGGAGC+AGG + tig0006360:2439-2458 Msa1343310:three_prime_UTR 45.0%
CATAAGTCACTCTCCACAAG+AGG + tig0006360:2610-2629 Msa1343310:three_prime_UTR 45.0%
TCGTTTAGCACATCCTCTTG+TGG - tig0006360:2626-2645 Msa1343320:CDS 45.0%
GCTAAACGATGATGTATGGG+CGG + tig0006360:2637-2656 Msa1343310:three_prime_UTR 45.0%
TAGTGTGTCAAGCAACTGGC+TGG + tig0006360:314-333 Msa1343310:five_prime_UTR 50.0%
CGGGATTCAGCATGGTGAAT+TGG - tig0006360:495-514 None:intergenic 50.0%
GAACTCGATAGAAGCTGTGG+AGG + tig0006360:822-841 Msa1343310:five_prime_UTR 50.0%
CTGCAGCTTCACATTGAGCA+GGG + tig0006360:995-1014 Msa1343310:intron 50.0%
GGTCCTGTGCTTGAACAAGT+TGG + tig0006360:1016-1035 Msa1343310:five_prime_UTR 50.0%
!! GGTGTCGTTAAAGGCATAGC+TGG + tig0006360:1049-1068 Msa1343310:five_prime_UTR 50.0%
AGCTGGGCAGACAAGATTGA+AGG + tig0006360:1066-1085 Msa1343310:five_prime_UTR 50.0%
GTCACGGTTAAAGGCTCACA+AGG + tig0006360:1183-1202 Msa1343310:five_prime_UTR 50.0%
AAAGGCTCACAAGGTCATGC+CGG + tig0006360:1192-1211 Msa1343310:exon 50.0%
GGATCTTGACGCATGGACAT+TGG - tig0006360:1225-1244 None:intergenic 50.0%
GTCCATGCGTCAAGATCCTA+TGG + tig0006360:1227-1246 Msa1343310:CDS 50.0%
CACCATAGGATCTTGACGCA+TGG - tig0006360:1232-1251 None:intergenic 50.0%
CATGCGTCAAGATCCTATGG+TGG + tig0006360:1230-1249 Msa1343310:CDS 50.0%
GGCAGAGAGACCTTACAAAC+CGG - tig0006360:1810-1829 None:intergenic 50.0%
!! CAAAACTGGCTTGTAAGGCG+AGG + tig0006360:1989-2008 Msa1343310:intron 50.0%
GTGGGCCTAAAGTAAGGACT+TGG + tig0006360:2212-2231 Msa1343310:intron 50.0%
GTGGCCCAAGTCCTTACTTT+AGG - tig0006360:2220-2239 None:intergenic 50.0%
TGTTCACCTCAGCATGATGC+AGG + tig0006360:2322-2341 Msa1343310:intron 50.0%
TTCTGCACTCAAGAGGATGG+AGG + tig0006360:2396-2415 Msa1343310:three_prime_UTR 50.0%
TCTGCACTCAAGAGGATGGA+GGG + tig0006360:2397-2416 Msa1343310:three_prime_UTR 50.0%
! ATGCTGTTTGTGCGATGTCG+CGG + tig0006360:2584-2603 Msa1343310:three_prime_UTR 50.0%
!! TTGGTTGGCCTCCTCTTGAT+AGG - tig0006360:2841-2860 Msa1343320:intron 50.0%
GCTGCAGCTTCACATTGAGC+AGG + tig0006360:994-1013 Msa1343310:intron 55.0%
TGTTCAGCAGCAGCCACCAT+AGG - tig0006360:1246-1265 None:intergenic 55.0%
AATTCATCGCTTGCCTGGCC+TGG - tig0006360:1420-1439 None:intergenic 55.0%
TGTGGCTTCTGTTCCAGCAG+AGG + tig0006360:2083-2102 Msa1343310:intron 55.0%
GATTGAGGTTCGACCTCTGC+TGG - tig0006360:2099-2118 None:intergenic 55.0%
ACTTGGGCCACCTTATGCCT+GGG + tig0006360:2229-2248 Msa1343310:intron 55.0%
ACAGCACCTGCATCATGCTG+AGG - tig0006360:2331-2350 None:intergenic 55.0%
AACGATGATGTATGGGCGGC+AGG + tig0006360:2641-2660 Msa1343310:three_prime_UTR 55.0%
CAGCTATAGCCCCACTGCCT+AGG - tig0006360:656-675 None:intergenic 60.0%
CTAGGCAGTGGGGCTATAGC+TGG + tig0006360:654-673 Msa1343310:five_prime_UTR 60.0%
GACTTGGGCCACCTTATGCC+TGG + tig0006360:2228-2247 Msa1343310:intron 60.0%
TGGAGCAGGGCATGATGCCA+TGG + tig0006360:2453-2472 Msa1343310:three_prime_UTR 60.0%
CTGTTTGTGCGATGTCGCGG+AGG + tig0006360:2587-2606 Msa1343310:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
tig0006360 gene 1 3139 1 ID=Msa1343310;Name=Msa1343310
tig0006360 mRNA 1 3139 1 ID=Msa1343310-mRNA-1;Parent=Msa1343310;Name=Msa1343310-mRNA-1;_AED=0.04;_eAED=0.04;_QI=710|1|1|1|0.85|0.62|8|705|156
tig0006360 exon 1 65 1 ID=Msa1343310-mRNA-1:exon:4096;Parent=Msa1343310-mRNA-1
tig0006360 exon 195 495 195 ID=Msa1343310-mRNA-1:exon:4097;Parent=Msa1343310-mRNA-1
tig0006360 exon 590 715 590 ID=Msa1343310-mRNA-1:exon:4098;Parent=Msa1343310-mRNA-1
tig0006360 exon 792 897 792 ID=Msa1343310-mRNA-1:exon:4099;Parent=Msa1343310-mRNA-1
tig0006360 exon 1001 1087 1001 ID=Msa1343310-mRNA-1:exon:4100;Parent=Msa1343310-mRNA-1
tig0006360 exon 1183 1659 1183 ID=Msa1343310-mRNA-1:exon:4101;Parent=Msa1343310-mRNA-1
tig0006360 exon 2334 2495 2334 ID=Msa1343310-mRNA-1:exon:4102;Parent=Msa1343310-mRNA-1
tig0006360 exon 2578 3139 2578 ID=Msa1343310-mRNA-1:exon:4103;Parent=Msa1343310-mRNA-1
tig0006360 five_prime_UTR 1 65 1 ID=Msa1343310-mRNA-1:five_prime_utr;Parent=Msa1343310-mRNA-1
tig0006360 five_prime_UTR 195 495 195 ID=Msa1343310-mRNA-1:five_prime_utr;Parent=Msa1343310-mRNA-1
tig0006360 five_prime_UTR 590 715 590 ID=Msa1343310-mRNA-1:five_prime_utr;Parent=Msa1343310-mRNA-1
tig0006360 five_prime_UTR 792 897 792 ID=Msa1343310-mRNA-1:five_prime_utr;Parent=Msa1343310-mRNA-1
tig0006360 five_prime_UTR 1001 1087 1001 ID=Msa1343310-mRNA-1:five_prime_utr;Parent=Msa1343310-mRNA-1
tig0006360 five_prime_UTR 1183 1207 1183 ID=Msa1343310-mRNA-1:five_prime_utr;Parent=Msa1343310-mRNA-1
tig0006360 CDS 1208 1659 1208 ID=Msa1343310-mRNA-1:cds;Parent=Msa1343310-mRNA-1
tig0006360 CDS 2334 2352 2334 ID=Msa1343310-mRNA-1:cds;Parent=Msa1343310-mRNA-1
tig0006360 three_prime_UTR 2353 2495 2353 ID=Msa1343310-mRNA-1:three_prime_utr;Parent=Msa1343310-mRNA-1
tig0006360 three_prime_UTR 2578 3139 2578 ID=Msa1343310-mRNA-1:three_prime_utr;Parent=Msa1343310-mRNA-1
Gene Sequence

>Msa1343310

ATGCCGGAACTGTTCCAATGTCCATGCGTCAAGATCCTATGGTGGCTGCTGCTGAACAAATTGTACTTATGGAAAGCCTCTGCAAACATCCTGAAGAGTACCTTTCTTACGATGGTCGTTGCAGCGATTCATCGATTAAATCACTTTCAAGTTCACTTGTTTGTACTGTTGGTGAAATATCAACATGGCCAAGTGCTAGTAATGTCATTCCAGGCCAGGCAAGCGATGAATTTCCAAAAAGCTGTTGATCTGGTGATCTTCAAGTGGATATGTTTTTTGCTTTCTCAATTTGAAGCTTGTGATGATAATGTGATGATTGGTTGCAGGTTACATATACCGTGGATATACGAGCAATTGATGATCTTGGACGTGAGGCTGTTATCTATGATTTATCCAATCGAATTTATCAGATATGTGACAAGCGTTCAGTTTCCTGCCTTATTGAACACAAGCATGATGCAGGTGCTGTGA
Protein sequence

>Msa1343310

MPELFQCPCVKILWWLLLNKLYLWKASANILKSTFLTMVVAAIHRLNHFQVHLFVLLVKYQHGQVLVMSFQARQAMNFQKAVDLVIFKWICFLLSQFEACDDNVMIGCRLHIPWIYEQLMILDVRLLSMIYPIEFIRYVTSVQFPALLNTSMMQVL*