AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0232970

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0232970 MtrunA17_Chr2g0280381 84.015 269 14 1 1 240 1 269 4.71e-157 446
Msa0232970 MtrunA17_Chr2g0280381 67.490 243 76 3 230 471 9 249 8.17e-109 323
Msa0232970 MtrunA17_Chr2g0280361 88.797 241 27 0 230 470 9 249 5.73e-157 446
Msa0232970 MtrunA17_Chr2g0280361 68.266 271 53 5 1 240 1 269 5.12e-122 357
Msa0232970 MtrunA17_Chr2g0280391 70.850 247 67 2 230 471 9 255 2.32e-123 361
Msa0232970 MtrunA17_Chr2g0280391 63.669 278 63 4 1 240 1 278 1.54e-115 341
Msa0232970 MtrunA17_Chr6g0474901 25.322 466 271 13 52 464 44 485 6.70e-36 141
Msa0232970 MtrunA17_Chr6g0474901 29.130 230 147 4 257 472 26 253 1.90e-24 107
Msa0232970 MtrunA17_Chr6g0475201 25.731 513 294 17 11 464 8 492 1.15e-34 138
Msa0232970 MtrunA17_Chr6g0475201 28.627 255 167 7 230 472 8 259 7.39e-22 99.4
Msa0232970 MtrunA17_Chr6g0475091 26.186 485 274 15 38 464 43 501 4.56e-34 136
Msa0232970 MtrunA17_Chr6g0474871 25.054 463 271 16 52 462 49 487 4.37e-31 127
Msa0232970 MtrunA17_Chr6g0474871 31.220 205 132 4 272 467 49 253 7.31e-24 105
Msa0232970 MtrunA17_Chr6g0475121 25.835 449 258 15 68 464 67 492 2.65e-30 124
Msa0232970 MtrunA17_Chr6g0475121 27.835 194 131 3 288 472 67 260 4.65e-16 80.9
Msa0232970 MtrunA17_Chr3g0081031 31.963 219 131 4 259 464 47 260 1.70e-29 122
Msa0232970 MtrunA17_Chr3g0081031 25.909 220 108 5 41 213 49 260 3.72e-15 78.2
Msa0232970 MtrunA17_Chr6g0475001 24.314 510 302 15 11 462 6 489 4.35e-29 120
Msa0232970 MtrunA17_Chr6g0475001 29.565 230 148 4 257 472 31 260 7.52e-22 99.0
Msa0232970 MtrunA17_Chr3g0081051 32.995 197 118 3 274 461 48 239 7.36e-29 114
Msa0232970 MtrunA17_Chr3g0081051 24.779 226 132 5 23 210 14 239 5.04e-16 77.8
Msa0232970 MtrunA17_Chr6g0474811 25.000 492 285 17 36 469 34 499 5.42e-28 118
Msa0232970 MtrunA17_Chr6g0474811 27.919 197 126 4 288 470 65 259 6.16e-16 80.9
Msa0232970 MtrunA17_Chr8g0354271 29.075 227 138 6 259 470 38 256 3.54e-27 115
Msa0232970 MtrunA17_Chr8g0354271 24.427 262 142 9 1 219 8 256 4.85e-17 84.0
Msa0232970 MtrunA17_Chr2g0323011 31.197 234 152 6 234 461 13 243 8.84e-27 108
Msa0232970 MtrunA17_Chr3g0081011 30.348 201 124 5 271 462 51 244 2.61e-26 112
Msa0232970 MtrunA17_Chr3g0081001 28.019 207 142 4 261 462 41 245 3.86e-26 112
Msa0232970 MtrunA17_Chr6g0475111 24.561 456 268 18 68 468 73 507 4.83e-25 108
Msa0232970 MtrunA17_Chr8g0347571 25.275 455 265 17 61 465 61 490 1.13e-24 107
Msa0232970 MtrunA17_Chr8g0347571 26.368 201 133 6 281 475 61 252 1.07e-12 70.5
Msa0232970 MtrunA17_Chr5g0428591 28.936 235 161 3 233 462 11 244 5.19e-24 101
Msa0232970 MtrunA17_Chr5g0428591 27.317 205 113 5 42 211 41 244 7.29e-12 65.9
Msa0232970 MtrunA17_Chr1g0206331 32.039 206 127 5 273 467 54 257 7.51e-24 105
Msa0232970 MtrunA17_Chr1g0206251 25.188 532 292 23 2 464 17 511 1.00e-23 104
Msa0232970 MtrunA17_Chr8g0354351 30.286 175 119 2 290 462 69 242 8.95e-23 101
Msa0232970 MtrunA17_Chr1g0206531 29.596 223 145 3 260 473 42 261 1.34e-22 101
Msa0232970 MtrunA17_Chr1g0206461 29.091 220 147 3 260 473 42 258 2.43e-22 100
Msa0232970 MtrunA17_Chr2g0322981 29.508 183 121 4 258 432 32 214 5.33e-22 95.1
Msa0232970 MtrunA17_Chr1g0206351 30.045 223 142 6 254 465 37 256 6.56e-22 99.0
Msa0232970 MtrunA17_Chr1g0206571 31.481 216 138 5 260 466 47 261 1.03e-21 95.5
Msa0232970 MtrunA17_Chr5g0428571 29.808 208 140 2 260 462 39 245 1.75e-21 97.4
Msa0232970 MtrunA17_Chr6g0474971 29.362 235 140 5 257 472 26 253 3.03e-21 94.4
Msa0232970 MtrunA17_Chr1g0205771 30.435 207 138 3 262 462 52 258 6.79e-21 95.9
Msa0232970 MtrunA17_Chr7g0234231 27.064 218 145 5 255 462 24 237 1.62e-20 90.9
Msa0232970 MtrunA17_Chr8g0354421 26.860 242 161 7 235 462 30 269 5.18e-19 90.1
Msa0232970 MtrunA17_Chr8g0354411 30.723 166 113 2 300 463 83 248 6.25e-19 87.4
Msa0232970 MtrunA17_Chr6g0474861 31.841 201 121 6 272 462 49 243 8.90e-19 89.4
Msa0232970 MtrunA17_Chr5g0394011 30.049 203 129 8 271 462 51 251 1.34e-18 89.0
Msa0232970 MtrunA17_Chr8g0354341 25.373 201 136 3 264 462 57 245 1.58e-18 85.5
Msa0232970 MtrunA17_Chr1g0154371 28.000 250 156 7 230 462 24 266 3.38e-18 87.8
Msa0232970 MtrunA17_Chr1g0154311 29.524 210 141 3 260 462 43 252 1.31e-17 85.9
Msa0232970 MtrunA17_Chr1g0206521 29.016 193 128 3 287 473 712 901 1.34e-17 86.3
Msa0232970 MtrunA17_Chr1g0206521 29.016 193 128 4 287 473 70 259 2.25e-16 82.4
Msa0232970 MtrunA17_Chr1g0206291 29.600 250 155 10 233 466 24 268 1.62e-17 85.1
Msa0232970 MtrunA17_Chr5g0428531 26.168 214 147 3 260 463 36 248 4.00e-17 82.0
Msa0232970 MtrunA17_Chr8g0354281 25.967 181 127 4 288 462 67 246 1.13e-16 82.8
Msa0232970 MtrunA17_Chr1g0206361 29.717 212 133 7 271 470 53 260 8.79e-16 80.1
Msa0232970 MtrunA17_Chr1g0154361 27.861 201 135 5 271 462 52 251 9.89e-16 78.2
Msa0232970 MtrunA17_Chr1g0154351 27.861 201 136 4 271 462 52 252 3.90e-15 78.2
Msa0232970 MtrunA17_Chr8g0354441 26.506 166 118 3 300 462 91 255 4.28e-15 77.8
Msa0232970 MtrunA17_Chr1g0206381 26.180 233 145 6 250 465 32 254 5.03e-15 76.3
Msa0232970 MtrunA17_Chr3g0119041 25.126 199 125 5 286 462 65 261 5.11e-15 77.8
Msa0232970 MtrunA17_Chr1g0154341 26.389 216 142 5 260 462 41 252 6.41e-15 75.1
Msa0232970 MtrunA17_Chr1g0206231 26.768 198 139 5 271 462 56 253 1.24e-14 76.6
Msa0232970 MtrunA17_Chr5g0394001 27.477 222 144 7 255 462 31 249 4.25e-14 74.7
Msa0232970 MtrunA17_Chr5g0394021 27.869 183 119 6 288 462 70 247 1.32e-13 73.2
Msa0232970 MtrunA17_Chr1g0206401 24.783 230 144 6 254 465 31 249 1.53e-13 73.2
Msa0232970 MtrunA17_Chr8g0354431 26.519 181 119 5 292 462 71 247 2.95e-13 72.0
Msa0232970 MtrunA17_Chr1g0206451 29.189 185 117 5 287 461 104 284 3.47e-13 72.0
Msa0232970 MtrunA17_Chr5g0426941 24.779 226 151 6 260 470 27 248 4.40e-13 71.6
Msa0232970 MtrunA17_Chr5g0393981 25.346 217 151 4 262 471 114 326 8.94e-13 70.9
Msa0232970 MtrunA17_Chr1g0206581 29.208 202 123 9 287 470 88 287 9.90e-13 68.6
Msa0232970 MtrunA17_Chr1g0154321 25.743 202 130 5 271 456 52 249 3.79e-12 68.9
Msa0232970 MtrunA17_Chr1g0206601 26.943 193 131 5 287 470 92 283 6.78e-12 66.2
Msa0232970 MtrunA17_Chr1g0206201 23.858 197 144 3 272 462 57 253 1.70e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0232970 AT4G23170.1 34.314 204 115 7 271 462 49 245 1.83e-21 94.4
Msa0232970 AT4G23180.3 33.023 215 122 7 260 462 49 253 2.19e-21 97.4
Msa0232970 AT4G23180.3 30.986 213 107 7 37 211 43 253 1.54e-13 73.2
Msa0232970 AT4G23180.1 32.870 216 123 7 259 462 48 253 2.63e-21 97.8
Msa0232970 AT4G23180.1 30.986 213 107 7 37 211 43 253 1.86e-13 73.2
Msa0232970 AT4G23180.2 32.870 216 123 7 259 462 48 253 2.70e-21 97.4
Msa0232970 AT4G23180.2 30.986 213 107 7 37 211 43 253 1.91e-13 73.2
Msa0232970 AT4G05200.2 30.882 204 124 8 271 462 68 266 3.01e-20 94.4
Msa0232970 AT4G05200.1 30.882 204 124 8 271 462 50 248 3.16e-20 94.4
Msa0232970 AT5G48540.1 28.311 219 148 5 262 473 36 252 1.02e-18 86.3
Msa0232970 AT3G22060.1 29.208 202 133 6 268 461 49 248 1.07e-18 85.9
Msa0232970 AT4G23160.3 32.212 208 114 7 271 462 646 842 1.25e-18 89.7
Msa0232970 AT4G23160.2 32.212 208 114 7 271 462 646 842 1.25e-18 89.7
Msa0232970 AT4G23160.1 32.212 208 114 7 271 462 66 262 1.93e-18 89.0
Msa0232970 AT4G23270.2 29.101 189 125 3 287 469 64 249 7.03e-18 87.0
Msa0232970 AT4G23270.1 29.101 189 125 3 287 469 64 249 7.03e-18 87.0
Msa0232970 AT4G23270.3 29.101 189 125 3 287 469 64 249 8.20e-18 86.7
Msa0232970 AT4G38830.1 28.436 211 136 5 266 467 46 250 3.11e-17 85.1
Msa0232970 AT1G61750.1 28.293 205 121 4 267 457 49 241 9.10e-17 82.0
Msa0232970 AT4G23140.1 31.217 189 108 6 287 462 67 246 1.01e-16 83.6
Msa0232970 AT4G23280.2 29.319 191 122 4 287 469 66 251 1.06e-16 83.6
Msa0232970 AT4G23280.1 29.319 191 122 4 287 469 66 251 1.07e-16 83.6
Msa0232970 AT4G23140.2 31.217 189 108 6 287 462 67 246 1.12e-16 83.2
Msa0232970 AT4G23280.3 29.319 191 122 4 287 469 66 251 1.21e-16 83.2
Msa0232970 AT4G23150.1 30.366 191 107 6 287 462 66 245 1.46e-16 82.8
Msa0232970 AT4G23130.3 28.704 216 143 6 255 462 32 244 2.00e-16 82.4
Msa0232970 AT4G23130.1 28.704 216 143 6 255 462 32 244 2.29e-16 82.4
Msa0232970 AT4G23130.2 28.704 216 143 6 255 462 32 244 2.54e-16 82.0
Msa0232970 AT4G23240.1 28.713 202 133 4 272 463 46 246 2.75e-16 82.0
Msa0232970 AT3G58310.1 24.615 260 181 7 215 465 4 257 9.99e-16 77.4
Msa0232970 AT3G45860.1 31.016 187 109 6 287 462 70 247 1.45e-15 79.7
Msa0232970 AT4G23310.2 32.919 161 103 3 273 428 164 324 1.75e-15 79.7
Msa0232970 AT4G23310.1 32.919 161 103 3 273 428 164 324 1.85e-15 79.7
Msa0232970 AT4G23230.1 30.814 172 105 3 271 432 47 214 7.84e-15 77.4
Msa0232970 AT4G21410.2 29.412 187 117 7 287 462 68 250 9.27e-15 77.0
Msa0232970 AT4G21410.3 29.412 187 117 7 287 462 68 250 1.14e-14 77.0
Msa0232970 AT5G41290.1 25.121 207 147 2 260 459 39 244 2.02e-14 73.9
Msa0232970 AT4G11521.1 25.123 203 132 2 274 461 44 241 5.17e-14 72.4
Msa0232970 AT4G04500.1 26.087 230 144 7 256 462 27 253 7.41e-14 74.3
Msa0232970 AT4G04500.2 25.551 227 149 6 256 462 27 253 7.41e-14 74.3
Msa0232970 AT4G04500.3 25.551 227 149 6 256 462 27 253 7.41e-14 74.3
Msa0232970 AT2G31620.1 26.050 238 138 8 249 461 28 252 8.93e-14 71.6
Msa0232970 AT1G63580.1 24.893 233 155 7 255 468 17 248 1.38e-13 71.2
Msa0232970 AT4G11460.1 25.000 208 136 4 272 461 45 250 1.85e-13 73.2
Msa0232970 AT4G11460.3 25.000 208 136 4 272 461 45 250 1.86e-13 73.2
Msa0232970 AT4G11530.1 24.020 204 141 3 272 461 43 246 1.94e-13 73.2
Msa0232970 AT4G04570.1 25.366 205 139 4 272 462 47 251 2.13e-13 73.2
Msa0232970 AT4G04570.2 25.366 205 139 4 272 462 47 251 3.05e-13 72.4
Msa0232970 AT4G20670.1 24.883 213 138 5 271 466 51 258 9.30e-13 68.9
Msa0232970 AT4G23190.2 24.138 203 138 5 274 461 47 248 1.05e-12 70.9
Msa0232970 AT4G23200.2 24.138 203 141 4 272 461 41 243 1.13e-12 70.5
Msa0232970 AT4G23190.1 24.020 204 137 5 274 461 47 248 1.15e-12 70.9
Msa0232970 AT4G20640.1 24.645 211 137 5 271 464 51 256 1.39e-12 68.2
Msa0232970 AT4G20630.1 24.645 211 137 5 271 464 51 256 1.39e-12 68.2
Msa0232970 AT4G20580.1 24.645 211 137 5 271 464 51 256 1.39e-12 68.2
Msa0232970 AT4G20600.1 24.645 211 137 5 271 464 51 256 1.39e-12 68.2
Msa0232970 AT4G20620.1 24.645 211 137 5 271 464 51 256 1.39e-12 68.2
Msa0232970 AT4G20610.1 24.645 211 137 5 271 464 51 256 1.39e-12 68.2
Msa0232970 AT4G20590.1 24.645 211 137 5 271 464 51 256 1.39e-12 68.2
Msa0232970 AT4G23200.1 24.138 203 141 4 272 461 41 243 1.44e-12 70.5
Msa0232970 AT4G20570.1 24.645 211 137 5 271 464 65 270 2.41e-12 67.8
Msa0232970 AT4G20560.1 24.645 211 137 5 271 464 65 270 2.41e-12 67.8
Msa0232970 AT4G20540.1 24.645 211 137 5 271 464 65 270 2.41e-12 67.8
Msa0232970 AT4G20645.1 24.645 211 137 5 271 464 65 270 2.41e-12 67.8
Msa0232970 AT4G20530.1 24.645 211 137 5 271 464 65 270 2.41e-12 67.8
Msa0232970 AT4G20550.1 24.645 211 137 5 271 464 65 270 2.41e-12 67.8
Msa0232970 AT5G41300.1 25.714 210 136 5 260 456 43 245 3.04e-12 67.8
Msa0232970 AT4G23300.1 23.529 204 140 3 274 461 47 250 6.34e-12 68.6
Msa0232970 AT4G23210.1 23.039 204 141 3 274 461 47 250 8.11e-12 67.8
Msa0232970 AT4G23210.2 23.039 204 141 3 274 461 47 250 1.25e-11 67.4
Msa0232970 AT4G23210.4 23.039 204 141 3 274 461 47 250 1.25e-11 67.4
Msa0232970 AT4G23210.5 23.039 204 141 3 274 461 47 250 1.33e-11 67.0
Msa0232970 AT4G23210.3 23.039 204 141 3 274 461 47 250 1.42e-11 67.4
Msa0232970 AT4G21400.3 25.683 183 129 3 287 462 70 252 1.55e-11 67.0
Msa0232970 AT4G21400.6 25.683 183 129 3 287 462 70 252 1.59e-11 67.0
Msa0232970 AT4G21400.4 25.683 183 129 3 287 462 70 252 1.59e-11 67.0
Msa0232970 AT4G21400.5 25.683 183 129 3 287 462 70 252 1.59e-11 67.0
Msa0232970 AT4G21400.2 25.683 183 129 3 287 462 70 252 1.74e-11 67.0
Msa0232970 AT4G21400.1 25.683 183 129 3 287 462 70 252 1.90e-11 67.0
Msa0232970 AT4G11490.1 24.762 210 129 6 272 461 45 245 2.97e-11 66.2
Msa0232970 AT3G21940.1 24.510 204 121 7 283 461 77 272 5.97e-11 63.5
Msa0232970 AT4G23220.1 23.182 220 148 5 259 461 25 240 6.66e-11 65.1
Msa0232970 AT4G23250.2 26.570 207 132 5 54 240 748 954 6.84e-11 65.5
Msa0232970 AT4G04540.1 24.537 216 135 6 269 463 47 255 8.59e-11 64.7
Msa0232970 AT4G04540.4 24.537 216 135 6 269 463 47 255 9.05e-11 64.7
Msa0232970 AT4G04540.3 24.537 216 135 6 269 463 47 255 9.86e-11 64.3

Find 86 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATACAAGATGGGAAATTTA+TGG 0.216837 2_2:-5273650 Msa0232970:CDS
GAAAACTCCAGCAGGATTTA+TGG 0.230555 2_2:-5274085 Msa0232970:CDS
AAAAGAAGATGGGAAATTTA+TGG 0.230790 2_2:-5270434 Msa0232970:CDS
GAAAACTCCAGCAGGGTTTA+TGG 0.231939 2_2:-5270875 Msa0232970:CDS
TGTTATTAGCTTCTTTGTTC+TGG 0.237731 2_2:-5274278 Msa0232970:CDS
GATGATACTAATAGAGAATT+AGG 0.263034 2_2:-5274226 Msa0232970:CDS
ACTGTAACATCCCTATTCTT+AGG 0.284553 2_2:+5270767 None:intergenic
GATGACACCAATAGAGAAAT+AGG 0.298235 2_2:-5271013 Msa0232970:CDS
TGATGTTGATGACCCTTCTT+TGG 0.300916 2_2:-5270657 Msa0232970:CDS
CAAGAGAATTTAATAAGGTT+TGG 0.309844 2_2:+5270945 None:intergenic
TATCGAACCTATTTCTCTAT+TGG 0.319857 2_2:+5271006 None:intergenic
TGAAGCAAGAGAATTTAATA+AGG 0.330511 2_2:+5270940 None:intergenic
CTTAGCAGCGATTGCGGTCT+CGG 0.344776 2_2:-5273056 Msa0232970:CDS
CAAATGATTGCCCTAAGAAT+AGG 0.358547 2_2:-5270778 Msa0232970:CDS
ATGTTACAGTTTGGTTTAGT+TGG 0.362652 2_2:-5270754 Msa0232970:CDS
TGGAGTAGCAGCTGCAGCTT+CGG 0.376748 2_2:-5270609 Msa0232970:CDS
AAGCAACTCTTGTGGAGTTA+AGG 0.377234 2_2:+5274011 None:intergenic
ATTATACCACATGAAACAAT+TGG 0.378881 2_2:+5270409 None:intergenic
TGAAGCAAGAGAATTCAATA+AGG 0.385195 2_2:+5274150 None:intergenic
TTTGTTCTGGTCTTTCCAAA+TGG 0.392240 2_2:-5274265 Msa0232970:CDS
GTTACTTTCAGAGCTACAAT+TGG 0.410684 2_2:-5270461 Msa0232970:CDS
AAATGATTGCCCTAAGAATA+GGG 0.417098 2_2:-5270777 Msa0232970:CDS
AAAGGACTGCAAAAGGTGTC+TGG 0.422008 2_2:-5273708 Msa0232970:CDS
CTGTAACATCCCTATTCTTA+GGG 0.428336 2_2:+5270768 None:intergenic
TTTGTTGTAGTCTTTCCAAA+TGG 0.439463 2_2:-5271051 Msa0232970:intron
AGAATAGGGATGTTACAGTT+TGG 0.445140 2_2:-5270763 Msa0232970:CDS
ATCATACCACATGAAACAAC+TGG 0.456202 2_2:+5273625 None:intergenic
GGACTCACCACTTGGAAGAA+AGG 0.462771 2_2:+5273577 None:intergenic
AAGGTGCCTTGATGATCAAC+TGG 0.473787 2_2:-5270483 Msa0232970:CDS
GATCCTTCTGTGGTTTCACA+AGG 0.475482 2_2:-5273860 Msa0232970:CDS
ATGTAATAAAGCTGATAATA+CGG 0.479573 2_2:-5270984 Msa0232970:CDS
AATCAAAGTGAGACGAGGTA+TGG 0.480046 2_2:-5270548 Msa0232970:CDS
TGCTCTGTGGTACAGCTGCA+TGG 0.486675 2_2:-5273099 Msa0232970:intron
CAGTGGTTTGGTAAAATCCT+TGG 0.488835 2_2:+5270903 None:intergenic
GACCCTTCTTTGGCATCACA+AGG 0.492204 2_2:-5270647 Msa0232970:CDS
AACCCTTGTGATGCCAAAGA+AGG 0.496026 2_2:+5270644 None:intergenic
TCAGTAGAAAGGACTGCAAA+AGG 0.496717 2_2:-5273715 Msa0232970:CDS
AATCAAAGTGAGAAGAGGTA+TGG 0.509596 2_2:-5273761 Msa0232970:CDS
TATTGAACCATAAATCCTGC+TGG 0.512356 2_2:+5274078 None:intergenic
CACAAGAGATATCAGTAGAA+AGG 0.513490 2_2:-5273726 Msa0232970:CDS
TATTGAACCATAAACCCTGC+TGG 0.521655 2_2:+5270868 None:intergenic
GCTAAGCTTCTGCAAGAAGA+AGG 0.526750 2_2:+5273073 None:intergenic
CAATTGGAAATAAAAGAAGA+TGG 0.531508 2_2:-5270445 Msa0232970:CDS
CTGTAGGAGAAAACTCCAGC+AGG 0.532218 2_2:-5270883 Msa0232970:CDS
CACAAGAGATATCAATAGAC+AGG 0.537570 2_2:-5270513 Msa0232970:CDS
TTATACCACATGAAACAATT+GGG 0.545504 2_2:+5270410 None:intergenic
AACATTTGAAGCAACTCTTG+TGG 0.552679 2_2:+5274003 None:intergenic
ATCAAAGTGAGACGAGGTAT+GGG 0.555037 2_2:-5270547 Msa0232970:CDS
TATGGTTCAATATTATGTAG+AGG 0.557939 2_2:-5274067 Msa0232970:CDS
CAAGGGTTACCTTTCATGAG+TGG 0.558522 2_2:-5273842 Msa0232970:CDS
CAATTAGAAATAATACAAGA+TGG 0.559075 2_2:-5273661 Msa0232970:CDS
AAATTCTCTTGCTTCAAATG+TGG 0.560501 2_2:-5270933 Msa0232970:CDS
ATGGATCCAGTTGTTTCATG+TGG 0.561659 2_2:-5273631 Msa0232970:CDS
TTGCAGGAGAAAACTCCAGC+AGG 0.567895 2_2:-5274093 Msa0232970:CDS
ATGGACCCAATTGTTTCATG+TGG 0.569094 2_2:-5270415 Msa0232970:CDS
CAGAAGCTTAGCAGCGATTG+CGG 0.571860 2_2:-5273062 Msa0232970:CDS
AATTGGAAATAAAAGAAGAT+GGG 0.576112 2_2:-5270444 Msa0232970:CDS
AAGAACTAATCAAAAGTCAA+AGG 0.579778 2_2:+5270340 None:intergenic
AATGTATCCTTTCTTCCAAG+TGG 0.580338 2_2:-5273584 Msa0232970:intron
TCAATAGACAGGACTGCAGA+AGG 0.581918 2_2:-5270502 Msa0232970:CDS
AATTAGAAATAATACAAGAT+GGG 0.583144 2_2:-5273660 Msa0232970:CDS
AAGTAACCAGTTGATCATCA+AGG 0.586503 2_2:+5270477 None:intergenic
CTCTATTAGTATCATCTGCA+AGG 0.590747 2_2:+5274233 None:intergenic
AATTCTCGTCAGAATAGCGT+AGG 0.592980 2_2:+5270726 None:intergenic
CAAGGGTTACCTTTCATGAG+TGG 0.594982 2_2:-5270629 Msa0232970:CDS
GCTGCTACTCCACTCATGAA+AGG 0.595456 2_2:+5270620 None:intergenic
AAGTGAGAAGAGGTATGGAA+TGG 0.598662 2_2:-5273756 Msa0232970:CDS
GCTGCTACTCCACTCATGAA+AGG 0.600644 2_2:+5273833 None:intergenic
ACCCTTGTGATGCCAAAGAA+GGG 0.604043 2_2:+5270645 None:intergenic
CAATGGTTTGGTAAAATCCA+TGG 0.606145 2_2:+5274113 None:intergenic
AATTTCTTCAGAGACATGCA+TGG 0.609462 2_2:-5270707 Msa0232970:CDS
ACTTCAATCAAAGTGAGAAG+AGG 0.610018 2_2:-5273766 Msa0232970:CDS
TGATATTGATGATCCTTCTG+TGG 0.611610 2_2:-5273870 Msa0232970:CDS
AATGTATCTACTCTTCAAAG+TGG 0.615207 2_2:-5270368 Msa0232970:CDS
GAATTCTCTTGCTTCAAACG+TGG 0.618324 2_2:-5274143 Msa0232970:CDS
AAGTGAGACGAGGTATGGGA+TGG 0.640024 2_2:-5270543 Msa0232970:CDS
ATCCTTCTGTGGTTTCACAA+GGG 0.648310 2_2:-5273859 Msa0232970:CDS
TGAAGTTTAGTTCTTCTGTG+TGG 0.649487 2_2:+5270570 None:intergenic
AACCCTTGTGAAACCACAGA+AGG 0.649733 2_2:+5273857 None:intergenic
ACCCTTCTTTGGCATCACAA+GGG 0.651646 2_2:-5270646 Msa0232970:CDS
TCAAATGTGGTTGATCACCA+AGG 0.662094 2_2:-5270920 Msa0232970:CDS
TCAAACGTGGTTGATCACCA+TGG 0.670200 2_2:-5274130 Msa0232970:CDS
TGTAGGAGAAAACTCCAGCA+GGG 0.684933 2_2:-5270882 Msa0232970:CDS
TGAAGTTCAGTACTTCTGTG+TGG 0.711031 2_2:+5273783 None:intergenic
ACTTCAATCAAAGTGAGACG+AGG 0.715455 2_2:-5270553 Msa0232970:CDS
TCTGTGGTACAGCTGCATGG+AGG 0.726756 2_2:-5273096 Msa0232970:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTTTATATTGTTAAATTTA+TGG + chr2_2:5272504-5272523 None:intergenic 10.0%
!! AATATAAAGAATTATGTATT+AGG - chr2_2:5272514-5272533 Msa0232970:intron 10.0%
!! AATTAGAAATAATACAAGAT+GGG - chr2_2:5270996-5271015 Msa0232970:CDS 15.0%
!! ACTTATAAGAATATCTTTAT+TGG - chr2_2:5271315-5271334 Msa0232970:intron 15.0%
!! TGGATAATATGTATTTATAT+AGG - chr2_2:5271335-5271354 Msa0232970:intron 15.0%
!!! ATGTTTCAGAAATATTTTAA+AGG - chr2_2:5272028-5272047 Msa0232970:intron 15.0%
!! GTTAATAATGTTTATCTTAA+TGG + chr2_2:5272390-5272409 None:intergenic 15.0%
!!! TTTTTATTTTTAACCCAATT+AGG + chr2_2:5272558-5272577 None:intergenic 15.0%
!! AAAAACAAAAAAAATTGACT+TGG + chr2_2:5272781-5272800 None:intergenic 15.0%
!! GACTATTATAACTAAAATTA+GGG + chr2_2:5273111-5273130 None:intergenic 15.0%
!! TGACTATTATAACTAAAATT+AGG + chr2_2:5273112-5273131 None:intergenic 15.0%
!! ACATTAAACAAAATGTTAAA+TGG - chr2_2:5273158-5273177 Msa0232970:intron 15.0%
!! CATTAAACAAAATGTTAAAT+GGG - chr2_2:5273159-5273178 Msa0232970:intron 15.0%
!! ATTTATATCAATTGATCAAA+TGG + chr2_2:5273489-5273508 None:intergenic 15.0%
!! CAATTAGAAATAATACAAGA+TGG - chr2_2:5270995-5271014 Msa0232970:CDS 20.0%
!! AAGCAAAAAATACATTTATC+TGG - chr2_2:5271208-5271227 Msa0232970:intron 20.0%
!! ATTAAAAGACTTGAAATTAC+TGG - chr2_2:5271410-5271429 Msa0232970:intron 20.0%
!!! AGCTCTAAGATATTTTTATA+TGG - chr2_2:5271480-5271499 Msa0232970:intron 20.0%
!! TACTTTGTAATTTCTTGATT+TGG - chr2_2:5271710-5271729 Msa0232970:intron 20.0%
!! AGAAAAACGAAAAAACTTAA+AGG + chr2_2:5272072-5272091 None:intergenic 20.0%
!!! AAGTTCTGAGATTTTAAATT+GGG + chr2_2:5272314-5272333 None:intergenic 20.0%
!!! TATAATTGTCGTTTATAAAC+GGG + chr2_2:5272631-5272650 None:intergenic 20.0%
!!! GTATAATTGTCGTTTATAAA+CGG + chr2_2:5272632-5272651 None:intergenic 20.0%
!!! TATTTCTTCCATTTCTTTTT+TGG - chr2_2:5272805-5272824 Msa0232970:intron 20.0%
!! ATATTTGACCAAAAAAGAAA+TGG + chr2_2:5272816-5272835 None:intergenic 20.0%
!! ATTATAACTAAAATTAGGGA+AGG + chr2_2:5273107-5273126 None:intergenic 20.0%
!! ATGTAATAAAGCTGATAATA+CGG - chr2_2:5273672-5273691 Msa0232970:CDS 20.0%
!! AATTGGAAATAAAAGAAGAT+GGG - chr2_2:5274212-5274231 Msa0232970:CDS 20.0%
! GATGATACTAATAGAGAATT+AGG - chr2_2:5270430-5270449 Msa0232970:CDS 25.0%
!! ATTTTCTTCATTCTTACATG+TGG + chr2_2:5270458-5270477 None:intergenic 25.0%
! TATGGTTCAATATTATGTAG+AGG - chr2_2:5270589-5270608 Msa0232970:CDS 25.0%
!!! ATGTTAGAGTTTTGTTTAGA+TGG - chr2_2:5270692-5270711 Msa0232970:CDS 25.0%
! AATACAAGATGGGAAATTTA+TGG - chr2_2:5271006-5271025 Msa0232970:CDS 25.0%
! AAGAACTAAACAAAAGTCAA+AGG + chr2_2:5271103-5271122 None:intergenic 25.0%
! AGCTATTATAATTGTTCACA+AGG - chr2_2:5271133-5271152 Msa0232970:intron 25.0%
!!! TGTACGTTTTGCTTTTATTT+AGG - chr2_2:5271272-5271291 Msa0232970:intron 25.0%
! GAATTAGAAAACTATCAACA+AGG + chr2_2:5271629-5271648 None:intergenic 25.0%
!!! TTTGTAATTTCTTGATTTGG+TGG - chr2_2:5271713-5271732 Msa0232970:intron 25.0%
!! TTAATTTTCTACTGATGTGA+AGG - chr2_2:5271967-5271986 Msa0232970:intron 25.0%
! TTGAAACTTGCCAAATTTAA+AGG + chr2_2:5272104-5272123 None:intergenic 25.0%
!!! AGTTCTGAGATTTTAAATTG+GGG + chr2_2:5272313-5272332 None:intergenic 25.0%
!!! GAAGTTCTGAGATTTTAAAT+TGG + chr2_2:5272315-5272334 None:intergenic 25.0%
! CGTTGTAATTCATACATTAT+GGG + chr2_2:5272364-5272383 None:intergenic 25.0%
! ACGTTGTAATTCATACATTA+TGG + chr2_2:5272365-5272384 None:intergenic 25.0%
!! AGATAAACTTGTTTATCGTA+AGG + chr2_2:5272412-5272431 None:intergenic 25.0%
! TTAACAAATGTAAAAACGAG+AGG - chr2_2:5272675-5272694 Msa0232970:intron 25.0%
! TAACAAATGTAAAAACGAGA+GGG - chr2_2:5272676-5272695 Msa0232970:intron 25.0%
!! CTTCTTTTGTCATATCTTAA+TGG - chr2_2:5272738-5272757 Msa0232970:intron 25.0%
! TTAGTCATTCAACTTAAGAA+AGG + chr2_2:5272844-5272863 None:intergenic 25.0%
!!! TACATGATATATTTTTGCGT+GGG + chr2_2:5272875-5272894 None:intergenic 25.0%
!!! ATACATGATATATTTTTGCG+TGG + chr2_2:5272876-5272895 None:intergenic 25.0%
! TGAGTTTATCTATAACTTCT+AGG + chr2_2:5273138-5273157 None:intergenic 25.0%
!!! ACTTTCTAAAGTTAACTCAA+AGG - chr2_2:5273199-5273218 Msa0232970:intron 25.0%
! TTGTATATGTGCTTGAAAAA+TGG + chr2_2:5273235-5273254 None:intergenic 25.0%
! GTTTATTTAACACGATAGAT+TGG - chr2_2:5273338-5273357 Msa0232970:intron 25.0%
! CAAGAGAATTTAATAAGGTT+TGG + chr2_2:5273714-5273733 None:intergenic 25.0%
! TGAAGCAAGAGAATTTAATA+AGG + chr2_2:5273719-5273738 None:intergenic 25.0%
! CAATTGGAAATAAAAGAAGA+TGG - chr2_2:5274211-5274230 Msa0232970:CDS 25.0%
! AAAAGAAGATGGGAAATTTA+TGG - chr2_2:5274222-5274241 Msa0232970:CDS 25.0%
! TTATACCACATGAAACAATT+GGG + chr2_2:5274249-5274268 None:intergenic 25.0%
! ATTATACCACATGAAACAAT+TGG + chr2_2:5274250-5274269 None:intergenic 25.0%
! TGTTATTAGCTTCTTTGTTC+TGG - chr2_2:5270378-5270397 Msa0232970:CDS 30.0%
TGAAGCAAGAGAATTCAATA+AGG + chr2_2:5270509-5270528 None:intergenic 30.0%
TTGCTTATATTTAAGTCGAG+AGG + chr2_2:5271194-5271213 None:intergenic 30.0%
ATAGACCAGTTAATTACTTG+CGG - chr2_2:5271238-5271257 Msa0232970:intron 30.0%
!! TTGCTTTTATTTAGGTAGAG+AGG - chr2_2:5271280-5271299 Msa0232970:intron 30.0%
GAGCAAAACTTATATTAGCT+TGG + chr2_2:5271542-5271561 None:intergenic 30.0%
! CTAATATAAGTTTTGCTCTG+TGG - chr2_2:5271544-5271563 Msa0232970:intron 30.0%
!! TGGTGGCTTTGTAAATAATA+TGG - chr2_2:5271730-5271749 Msa0232970:intron 30.0%
!! GGTGGCTTTGTAAATAATAT+GGG - chr2_2:5271731-5271750 Msa0232970:intron 30.0%
! TTTTACTCTTTATGCTACAG+AGG + chr2_2:5271796-5271815 None:intergenic 30.0%
TAGCATAAGTAAATAGAAGC+TGG - chr2_2:5271835-5271854 Msa0232970:intron 30.0%
CTGAAGAAAGAGTGAAAATA+TGG - chr2_2:5271873-5271892 Msa0232970:intron 30.0%
TAAAGGCCAAATACATTCAA+AGG - chr2_2:5272045-5272064 Msa0232970:intron 30.0%
AAAGGACCTTTGAATGTATT+TGG + chr2_2:5272054-5272073 None:intergenic 30.0%
!!! TTTTTTCGTTTTTCTCATGC+TGG - chr2_2:5272077-5272096 Msa0232970:intron 30.0%
GGGTCTATTTAACAAGTTAA+TGG + chr2_2:5272192-5272211 None:intergenic 30.0%
TTCATACATTATGGGAAATG+AGG + chr2_2:5272356-5272375 None:intergenic 30.0%
AAATGTGTCCATAAACCAAA+AGG - chr2_2:5272575-5272594 Msa0232970:intron 30.0%
CAATTATACTCTTCAAGTCA+AGG - chr2_2:5272643-5272662 Msa0232970:intron 30.0%
ATATGACAAAAGAAGTTGTC+GGG + chr2_2:5272733-5272752 None:intergenic 30.0%
GATATGACAAAAGAAGTTGT+CGG + chr2_2:5272734-5272753 None:intergenic 30.0%
ACAATCTATGTAAAACTACG+TGG + chr2_2:5273051-5273070 None:intergenic 30.0%
TAAACGCTGACAATGTAAAA+TGG - chr2_2:5273267-5273286 Msa0232970:intron 30.0%
AAAAGCTAGTTCGAATGATA+AGG + chr2_2:5273303-5273322 None:intergenic 30.0%
AAATGACTTCATAGTCTCTT+TGG + chr2_2:5273555-5273574 None:intergenic 30.0%
! TTTGTTGTAGTCTTTCCAAA+TGG - chr2_2:5273605-5273624 Msa0232970:CDS 30.0%
! TATCGAACCTATTTCTCTAT+TGG + chr2_2:5273653-5273672 None:intergenic 30.0%
AAATTCTCTTGCTTCAAATG+TGG - chr2_2:5273723-5273742 Msa0232970:CDS 30.0%
AAATGATTGCCCTAAGAATA+GGG - chr2_2:5273879-5273898 Msa0232970:CDS 30.0%
! ATGTTACAGTTTGGTTTAGT+TGG - chr2_2:5273902-5273921 Msa0232970:intron 30.0%
AATGTATCTACTCTTCAAAG+TGG - chr2_2:5274288-5274307 Msa0232970:CDS 30.0%
! TTTGTTCTGGTCTTTCCAAA+TGG - chr2_2:5270391-5270410 Msa0232970:CDS 35.0%
!!! GTCTTTCCAAATGGCTTTTT+TGG - chr2_2:5270400-5270419 Msa0232970:CDS 35.0%
CTCTATTAGTATCATCTGCA+AGG + chr2_2:5270426-5270445 None:intergenic 35.0%
!! CAATGGTTTGGTAAAATCCA+TGG + chr2_2:5270546-5270565 None:intergenic 35.0%
TATTGAACCATAAATCCTGC+TGG + chr2_2:5270581-5270600 None:intergenic 35.0%
! AACATTTGAAGCAACTCTTG+TGG + chr2_2:5270656-5270675 None:intergenic 35.0%
ACTCTAACATCTCTTCACTT+AGG + chr2_2:5270682-5270701 None:intergenic 35.0%
TATTCAAACGAGAGCTTCTT+TGG - chr2_2:5270727-5270746 Msa0232970:CDS 35.0%
! TGATATTGATGATCCTTCTG+TGG - chr2_2:5270786-5270805 Msa0232970:CDS 35.0%
ACTTCAATCAAAGTGAGAAG+AGG - chr2_2:5270890-5270909 Msa0232970:CDS 35.0%
AATCAAAGTGAGAAGAGGTA+TGG - chr2_2:5270895-5270914 Msa0232970:CDS 35.0%
CACAAGAGATATCAGTAGAA+AGG - chr2_2:5270930-5270949 Msa0232970:CDS 35.0%
ATCATACCACATGAAACAAC+TGG + chr2_2:5271034-5271053 None:intergenic 35.0%
AATGTATCCTTTCTTCCAAG+TGG - chr2_2:5271072-5271091 Msa0232970:intron 35.0%
TTGTTCCGCAAGTAATTAAC+TGG + chr2_2:5271246-5271265 None:intergenic 35.0%
TCTCCTTGTGATGTCTAATA+AGG - chr2_2:5271513-5271532 Msa0232970:intron 35.0%
CTTCCTTATTAGACATCACA+AGG + chr2_2:5271519-5271538 None:intergenic 35.0%
GAATGTTACATGTGTCTTTG+CGG - chr2_2:5271753-5271772 Msa0232970:intron 35.0%
ATGTGTCTTTGCGGTAAAAA+TGG - chr2_2:5271762-5271781 Msa0232970:intron 35.0%
TCATGCTGGTCCTTTAAATT+TGG - chr2_2:5272091-5272110 Msa0232970:intron 35.0%
TTAATCAGAGACTGACAGAA+AGG + chr2_2:5272154-5272173 None:intergenic 35.0%
AAAGACAATGTTATACACGC+TGG + chr2_2:5272236-5272255 None:intergenic 35.0%
!! AACTCATTTTTCCCCTCAAT+TGG - chr2_2:5272266-5272285 Msa0232970:intron 35.0%
!! TTTAGGGTTTTCCAATTGAG+GGG + chr2_2:5272280-5272299 None:intergenic 35.0%
!! GTTTAGGGTTTTCCAATTGA+GGG + chr2_2:5272281-5272300 None:intergenic 35.0%
!! AGTTTAGGGTTTTCCAATTG+AGG + chr2_2:5272282-5272301 None:intergenic 35.0%
!!! ATGCTGAACCTTTTGGTTTA+TGG + chr2_2:5272586-5272605 None:intergenic 35.0%
TCAGCATTGCTTGAGATTTA+AGG - chr2_2:5272599-5272618 Msa0232970:intron 35.0%
GTTTACATACAAACCCATAG+CGG - chr2_2:5272698-5272717 Msa0232970:intron 35.0%
TCGAATGATAAGGATCTTCA+AGG + chr2_2:5273293-5273312 None:intergenic 35.0%
TATGAGCAAAGTCAACTCTT+GGG + chr2_2:5273433-5273452 None:intergenic 35.0%
TTATGAGCAAAGTCAACTCT+TGG + chr2_2:5273434-5273453 None:intergenic 35.0%
! AGTCTTTCCAAATGGCTTTT+TGG - chr2_2:5273613-5273632 Msa0232970:CDS 35.0%
GATGACACCAATAGAGAAAT+AGG - chr2_2:5273643-5273662 Msa0232970:CDS 35.0%
CAAATGATTGCCCTAAGAAT+AGG - chr2_2:5273878-5273897 Msa0232970:CDS 35.0%
CTGTAACATCCCTATTCTTA+GGG + chr2_2:5273891-5273910 None:intergenic 35.0%
ACTGTAACATCCCTATTCTT+AGG + chr2_2:5273892-5273911 None:intergenic 35.0%
AGAATAGGGATGTTACAGTT+TGG - chr2_2:5273893-5273912 Msa0232970:CDS 35.0%
AATTTCTTCAGAGACATGCA+TGG - chr2_2:5273949-5273968 Msa0232970:intron 35.0%
!! TGAAGTTTAGTTCTTCTGTG+TGG + chr2_2:5274089-5274108 None:intergenic 35.0%
CACAAGAGATATCAATAGAC+AGG - chr2_2:5274143-5274162 Msa0232970:CDS 35.0%
AAGTAACCAGTTGATCATCA+AGG + chr2_2:5274182-5274201 None:intergenic 35.0%
! GTTACTTTCAGAGCTACAAT+TGG - chr2_2:5274195-5274214 Msa0232970:CDS 35.0%
GAATTCTCTTGCTTCAAACG+TGG - chr2_2:5270513-5270532 Msa0232970:CDS 40.0%
! GGATTTTACCAAACCATTGC+AGG - chr2_2:5270547-5270566 Msa0232970:CDS 40.0%
! AGTTTTCTCCTGCAATGGTT+TGG + chr2_2:5270558-5270577 None:intergenic 40.0%
GAAAACTCCAGCAGGATTTA+TGG - chr2_2:5270571-5270590 Msa0232970:CDS 40.0%
AAGCAACTCTTGTGGAGTTA+AGG + chr2_2:5270648-5270667 None:intergenic 40.0%
ATCCTTCTGTGGTTTCACAA+GGG - chr2_2:5270797-5270816 Msa0232970:CDS 40.0%
TGAAGTTCAGTACTTCTGTG+TGG + chr2_2:5270876-5270895 None:intergenic 40.0%
! AAGTGAGAAGAGGTATGGAA+TGG - chr2_2:5270900-5270919 Msa0232970:CDS 40.0%
TCAGTAGAAAGGACTGCAAA+AGG - chr2_2:5270941-5270960 Msa0232970:CDS 40.0%
! ATGGATCCAGTTGTTTCATG+TGG - chr2_2:5271025-5271044 Msa0232970:CDS 40.0%
! AGGAGTGCATATTTCAGTTG+AGG + chr2_2:5271174-5271193 None:intergenic 40.0%
GTGCACTTGCAAATTTGTCA+TGG - chr2_2:5271670-5271689 Msa0232970:intron 40.0%
CACGTATAAACAGGATCCAA+TGG + chr2_2:5271993-5272012 None:intergenic 40.0%
ATCCTGTTTATACGTGCTAG+AGG - chr2_2:5271997-5272016 Msa0232970:intron 40.0%
TCCTGTTTATACGTGCTAGA+GGG - chr2_2:5271998-5272017 Msa0232970:intron 40.0%
! AGGGCAAAATGTTGCGTTTT+GGG + chr2_2:5272212-5272231 None:intergenic 40.0%
TTGGGGAACAACAGAGTTTA+GGG + chr2_2:5272296-5272315 None:intergenic 40.0%
ATTGGGGAACAACAGAGTTT+AGG + chr2_2:5272297-5272316 None:intergenic 40.0%
GAAATGAGGCAATGGAAAAC+TGG + chr2_2:5272342-5272361 None:intergenic 40.0%
CATTATGGGAAATGAGGCAA+TGG + chr2_2:5272350-5272369 None:intergenic 40.0%
ACATCTCATTGCGACCTAAT+TGG - chr2_2:5272541-5272560 Msa0232970:intron 40.0%
CATCTCATTGCGACCTAATT+GGG - chr2_2:5272542-5272561 Msa0232970:intron 40.0%
! TCAAGCAATGCTGAACCTTT+TGG + chr2_2:5272593-5272612 None:intergenic 40.0%
TGATTCGTTCATCGATCGTT+TGG - chr2_2:5272989-5273008 Msa0232970:intron 40.0%
CTATGTAAAACTACGTGGAG+TGG + chr2_2:5273046-5273065 None:intergenic 40.0%
CAAGAAGTAAGCCAGAACTT+TGG - chr2_2:5273072-5273091 Msa0232970:CDS 40.0%
AGCAATGCCAAAAAGCCATT+TGG + chr2_2:5273623-5273642 None:intergenic 40.0%
TCAAATGTGGTTGATCACCA+AGG - chr2_2:5273736-5273755 Msa0232970:CDS 40.0%
!! CAGTGGTTTGGTAAAATCCT+TGG + chr2_2:5273756-5273775 None:intergenic 40.0%
! GGATTTTACCAAACCACTGT+AGG - chr2_2:5273757-5273776 Msa0232970:CDS 40.0%
! AGTTTTCTCCTACAGTGGTT+TGG + chr2_2:5273768-5273787 None:intergenic 40.0%
TATTGAACCATAAACCCTGC+TGG + chr2_2:5273791-5273810 None:intergenic 40.0%
AATTCTCGTCAGAATAGCGT+AGG + chr2_2:5273933-5273952 None:intergenic 40.0%
!! TGATGTTGATGACCCTTCTT+TGG - chr2_2:5273999-5274018 Msa0232970:intron 40.0%
ACTTCAATCAAAGTGAGACG+AGG - chr2_2:5274103-5274122 Msa0232970:CDS 40.0%
AATCAAAGTGAGACGAGGTA+TGG - chr2_2:5274108-5274127 Msa0232970:CDS 40.0%
ATCAAAGTGAGACGAGGTAT+GGG - chr2_2:5274109-5274128 Msa0232970:CDS 40.0%
ATGGACCCAATTGTTTCATG+TGG - chr2_2:5274241-5274260 Msa0232970:CDS 40.0%
GCAAGGCCAAAAAAGCCATT+TGG + chr2_2:5270409-5270428 None:intergenic 45.0%
TCAAACGTGGTTGATCACCA+TGG - chr2_2:5270526-5270545 Msa0232970:CDS 45.0%
AGCTCTCGTTTGAATAGCGT+AGG + chr2_2:5270723-5270742 None:intergenic 45.0%
GATCCTTCTGTGGTTTCACA+AGG - chr2_2:5270796-5270815 Msa0232970:CDS 45.0%
AACCCTTGTGAAACCACAGA+AGG + chr2_2:5270802-5270821 None:intergenic 45.0%
CAAGGGTTACCTTTCATGAG+TGG - chr2_2:5270814-5270833 Msa0232970:CDS 45.0%
!! AAAGGACTGCAAAAGGTGTC+TGG - chr2_2:5270948-5270967 Msa0232970:CDS 45.0%
AAGTCAAAGGACTCACCACT+TGG + chr2_2:5271090-5271109 None:intergenic 45.0%
GCTAAGCTTCTGCAAGAAGA+AGG + chr2_2:5271586-5271605 None:intergenic 45.0%
TCTACTGATGTGAAGGCCAT+TGG - chr2_2:5271974-5271993 Msa0232970:intron 45.0%
CCTGTTTATACGTGCTAGAG+GGG - chr2_2:5271999-5272018 Msa0232970:intron 45.0%
! CAGGGCAAAATGTTGCGTTT+TGG + chr2_2:5272213-5272232 None:intergenic 45.0%
CAATGTTATACACGCTGGCA+GGG + chr2_2:5272231-5272250 None:intergenic 45.0%
ACAATGTTATACACGCTGGC+AGG + chr2_2:5272232-5272251 None:intergenic 45.0%
TGACAAAAGAAGTTGTCGGG+AGG + chr2_2:5272730-5272749 None:intergenic 45.0%
GGCTCAAATGACCAAAGTTC+TGG + chr2_2:5273086-5273105 None:intergenic 45.0%
GCAGAATAGCAGAAGAGAAG+AGG + chr2_2:5273528-5273547 None:intergenic 45.0%
TGTAGGAGAAAACTCCAGCA+GGG - chr2_2:5273774-5273793 Msa0232970:CDS 45.0%
GAAAACTCCAGCAGGGTTTA+TGG - chr2_2:5273781-5273800 Msa0232970:CDS 45.0%
ACCCTTCTTTGGCATCACAA+GGG - chr2_2:5274010-5274029 Msa0232970:CDS 45.0%
ACCCTTGTGATGCCAAAGAA+GGG + chr2_2:5274014-5274033 None:intergenic 45.0%
AACCCTTGTGATGCCAAAGA+AGG + chr2_2:5274015-5274034 None:intergenic 45.0%
CAAGGGTTACCTTTCATGAG+TGG - chr2_2:5270814-5270833 Msa0232970:CDS 45.0%
TCAATAGACAGGACTGCAGA+AGG - chr2_2:5274154-5274173 Msa0232970:CDS 45.0%
! AAGGTGCCTTGATGATCAAC+TGG - chr2_2:5274173-5274192 Msa0232970:CDS 45.0%
! GCTGGAGTTTTCTCCTGCAA+TGG + chr2_2:5270563-5270582 None:intergenic 50.0%
TTGCAGGAGAAAACTCCAGC+AGG - chr2_2:5270563-5270582 Msa0232970:CDS 50.0%
! GAGCTTCTTTGGAGACATGC+AGG - chr2_2:5270738-5270757 Msa0232970:CDS 50.0%
GCTGCTACTCCACTCATGAA+AGG + chr2_2:5270826-5270845 None:intergenic 50.0%
GGACTCACCACTTGGAAGAA+AGG + chr2_2:5271082-5271101 None:intergenic 50.0%
CAGAAGCTTAGCAGCGATTG+CGG - chr2_2:5271594-5271613 Msa0232970:intron 50.0%
CCCCTCTAGCACGTATAAAC+AGG + chr2_2:5272002-5272021 None:intergenic 50.0%
! GCTGGAGTTTTCTCCTACAG+TGG + chr2_2:5273773-5273792 None:intergenic 50.0%
CTGTAGGAGAAAACTCCAGC+AGG - chr2_2:5273773-5273792 Msa0232970:CDS 50.0%
GACCCTTCTTTGGCATCACA+AGG - chr2_2:5274009-5274028 Msa0232970:CDS 50.0%
GCTGCTACTCCACTCATGAA+AGG + chr2_2:5270826-5270845 None:intergenic 50.0%
AAGTGAGACGAGGTATGGGA+TGG - chr2_2:5274113-5274132 Msa0232970:CDS 50.0%
TGCTCTGTGGTACAGCTGCA+TGG - chr2_2:5271557-5271576 Msa0232970:intron 55.0%
TCTGTGGTACAGCTGCATGG+AGG - chr2_2:5271560-5271579 Msa0232970:intron 55.0%
CTTAGCAGCGATTGCGGTCT+CGG - chr2_2:5271600-5271619 Msa0232970:intron 55.0%
!! CGGGAGGTTTTGTCCGCTAT+GGG + chr2_2:5272714-5272733 None:intergenic 55.0%
!! TCGGGAGGTTTTGTCCGCTA+TGG + chr2_2:5272715-5272734 None:intergenic 55.0%
TGGAGTAGCAGCTGCAGCTT+CGG - chr2_2:5274047-5274066 Msa0232970:CDS 55.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 5270349 5274329 5270349 ID=Msa0232970;Name=Msa0232970
chr2_2 mRNA 5270349 5274329 5270349 ID=Msa0232970-mRNA-1;Parent=Msa0232970;Name=Msa0232970-mRNA-1;_AED=0.40;_eAED=0.40;_QI=0|0|0|1|1|1|4|0|476
chr2_2 exon 5274006 5274329 5274006 ID=Msa0232970-mRNA-1:exon:2295;Parent=Msa0232970-mRNA-1
chr2_2 exon 5273585 5273918 5273585 ID=Msa0232970-mRNA-1:exon:2294;Parent=Msa0232970-mRNA-1
chr2_2 exon 5273049 5273106 5273049 ID=Msa0232970-mRNA-1:exon:2293;Parent=Msa0232970-mRNA-1
chr2_2 exon 5270349 5271063 5270349 ID=Msa0232970-mRNA-1:exon:2292;Parent=Msa0232970-mRNA-1
chr2_2 CDS 5274006 5274329 5274006 ID=Msa0232970-mRNA-1:cds;Parent=Msa0232970-mRNA-1
chr2_2 CDS 5273585 5273918 5273585 ID=Msa0232970-mRNA-1:cds;Parent=Msa0232970-mRNA-1
chr2_2 CDS 5273049 5273106 5273049 ID=Msa0232970-mRNA-1:cds;Parent=Msa0232970-mRNA-1
chr2_2 CDS 5270349 5271063 5270349 ID=Msa0232970-mRNA-1:cds;Parent=Msa0232970-mRNA-1
Gene Sequence

>Msa0232970

ATGAAGACATTTTATTTTGTTATGCTATGTGTTATTAGCTTCTTTGTTCTGGTCTTTCCAAATGGCTTTTTTGGCCTTGCAGATGATACTAATAGAGAATTAGGTTCCACATGTAAGAATGAAGAAAATACTACTGTCAATGAAAACATATTGCAAACCTTATTGAATTCTCTTGCTTCAAACGTGGTTGATCACCATGGATTTTACCAAACCATTGCAGGAGAAAACTCCAGCAGGATTTATGGTTCAATATTATGTAGAGGAGACATATCTGCTAACAATTGTTCTGATTGTGTCCTTAACTCCACAAGAGTTGCTTCAAATGAAACAACAACTGCATATGACTTTGATGATATTGATGATCCTTCTGTGGTTTCACAAGGGTTACCTTTCATGAGTGGAGTAGCAGCTACAGCTTCAGAGAAATCATTAATGTTCCACACAGAAGTACTGAACTTCAATCAAAGTGAGAAGAGGTATGGAATGGCTCAGTGCACAAGAGATATCAGTAGAAAGGACTGCAAAAGGTGTCTGGATTCTCAGCTGATTAATCACAGAACAATTAGAAATAATACAAGATGGGAAATTTATGGATCCAGTTGTTTCATGTGGTATGATGACTATCAGTTTTATGCTAATGTATCCTTTCTTCCAAGTGCTGCATGGAGGCCTTCTTCTTGCAGAAGCTTAGCAGCGATTGCGGTCTCGGCAGCCTTTCTTTCCAAATGGCTTTTTGGCATTGCTGATGACACCAATAGAGAAATAGGTTCGATATGTAATAAAGCTGATAATACGGTCAATGAAGACATATTCCAAACCTTATTAAATTCTCTTGCTTCAAATGTGGTTGATCACCAAGGATTTTACCAAACCACTGTAGGAGAAAACTCCAGCAGGGTTTATGGTTCAATATTATGTAGACGAGACATATCTGCTAACAATTGTTCTGTTTGTGCTCTTAACTCTACAAGAGTAGCTTCAAATGATTGCCCTAAGAATAGGGATGTTACAGTTTGGTTTAGTTGGTGTTTCCTACGCTATTCTGACGAGAATTTCTTCAGAGACATGCATGGAATAACAAATGCATTCACAAATGACACTGATGTTGATGACCCTTCTTTGGCATCACAAGGGTTACCTTTCATGAGTGGAGTAGCAGCTGCAGCTTCGGAGAACTCATTCATGTTCCACACAGAAGAACTAAACTTCAATCAAAGTGAGACGAGGTATGGGATGGCTCAGTGCACAAGAGATATCAATAGACAGGACTGCAGAAGGTGCCTTGATGATCAACTGGTTACTTTCAGAGCTACAATTGGAAATAAAAGAAGATGGGAAATTTATGGACCCAATTGTTTCATGTGGTATAATGACTATCAATTTTATGCTAATGTATCTACTCTTCAAAGTGGTGAGTCCTTTGACTTTTGA
Protein sequence

>Msa0232970

MKTFYFVMLCVISFFVLVFPNGFFGLADDTNRELGSTCKNEENTTVNENILQTLLNSLASNVVDHHGFYQTIAGENSSRIYGSILCRGDISANNCSDCVLNSTRVASNETTTAYDFDDIDDPSVVSQGLPFMSGVAATASEKSLMFHTEVLNFNQSEKRYGMAQCTRDISRKDCKRCLDSQLINHRTIRNNTRWEIYGSSCFMWYDDYQFYANVSFLPSAAWRPSSCRSLAAIAVSAAFLSKWLFGIADDTNREIGSICNKADNTVNEDIFQTLLNSLASNVVDHQGFYQTTVGENSSRVYGSILCRRDISANNCSVCALNSTRVASNDCPKNRDVTVWFSWCFLRYSDENFFRDMHGITNAFTNDTDVDDPSLASQGLPFMSGVAAAASENSFMFHTEELNFNQSETRYGMAQCTRDINRQDCRRCLDDQLVTFRATIGNKRRWEIYGPNCFMWYNDYQFYANVSTLQSGESFDF*