AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0515150


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0515150 MtrunA17_Chr3g0124141 86.935 199 26 0 1 199 1 199 1.14e-130 365
Msa0515150 MtrunA17_Chr3g0124111 85.930 199 28 0 1 199 1 199 1.81e-130 364
Msa0515150 MtrunA17_Chr3g0124091 83.582 201 30 2 1 199 1 200 2.73e-124 349
Msa0515150 MtrunA17_Chr3g0124061 82.915 199 34 0 1 199 1 199 6.78e-124 348
Msa0515150 MtrunA17_Chr3g0124151 82.075 106 19 0 94 199 1 106 1.18e-59 182
Msa0515150 MtrunA17_Chr3g0124131 88.235 51 6 0 1 51 1 51 3.15e-27 97.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0515150 AT5G65030.1 31.980 197 109 7 11 187 10 201 2.04e-25 98.2
Msa0515150 AT5G10210.1 31.633 196 112 7 15 199 13 197 6.00e-24 94.4

Find 37 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGCCAAATCATTGCCCTTT+TGG 0.190841 3_4:-66168297 Msa0515150:CDS
ATATGGGGCTCCAAACTAAT+TGG 0.223958 3_4:-66168181 Msa0515150:CDS
ACACTAGGCTTTGTTGATTA+AGG 0.234215 3_4:+66168224 None:intergenic
CTCACCTTTCCCAATTAGTT+TGG 0.258127 3_4:+66168171 None:intergenic
TTAATCTCCACTTCCACTTC+TGG 0.281665 3_4:+66168043 None:intergenic
AGCTAAGGCAAAGGAAGATA+TGG 0.333930 3_4:-66168198 Msa0515150:CDS
TGCGCTTGGCGCAGCTTTGG+AGG 0.349292 3_4:-66167891 Msa0515150:CDS
GTCCCAAAAGGGCAATGATT+TGG 0.392085 3_4:+66168294 None:intergenic
ATTTGCGCTTGGCGCAGCTT+TGG 0.402289 3_4:-66167894 Msa0515150:CDS
GATTCAAGAATCACTTTCCA+TGG 0.437928 3_4:+66168145 None:intergenic
TATGGGGCTCCAAACTAATT+GGG 0.456932 3_4:-66168180 Msa0515150:CDS
GCTAAGGCAAAGGAAGATAT+GGG 0.457270 3_4:-66168197 Msa0515150:CDS
AAAATGGTTGAAGATGGATT+TGG 0.470551 3_4:-66168104 Msa0515150:CDS
AGTAGCTTCGGTTGCTGAGA+TGG 0.471131 3_4:-66168014 Msa0515150:CDS
GATTAAAATAAAAGTAGCTT+CGG 0.474275 3_4:-66168026 Msa0515150:CDS
ACTAAATGACTCGTCCCAAA+AGG 0.477554 3_4:+66168282 None:intergenic
TTGGGAAAGGTGAGATTCCA+TGG 0.490587 3_4:-66168162 Msa0515150:CDS
AAAGCCTAGTGTTGGAGCTA+AGG 0.499139 3_4:-66168213 Msa0515150:CDS
TTTGCCTTAGCTCCAACACT+AGG 0.507447 3_4:+66168209 None:intergenic
AGATGGAGAAGCAAAATAAG+AGG 0.509817 3_4:-66167997 Msa0515150:CDS
CTAAATGACTCGTCCCAAAA+GGG 0.528554 3_4:+66168283 None:intergenic
ATGAATAATTTGAATGAGTG+TGG 0.533229 3_4:-66167971 Msa0515150:CDS
ACGGCCATGACCATCGTGCT+TGG 0.538014 3_4:-66167940 Msa0515150:CDS
CCAAAAGGGCAATGATTTGG+CGG 0.551334 3_4:+66168297 None:intergenic
GATTGTGATATATTTGCGCT+TGG 0.560902 3_4:-66167905 Msa0515150:CDS
TAATCAACAAAGCCTAGTGT+TGG 0.564525 3_4:-66168221 Msa0515150:CDS
AGCACCAAGCACGATGGTCA+TGG 0.566327 3_4:+66167936 None:intergenic
CATAACGCCAGAAGTGGAAG+TGG 0.570407 3_4:-66168050 Msa0515150:CDS
GATTCTTGAATCACAAAACA+TGG 0.583125 3_4:-66168134 Msa0515150:CDS
TATGTTCATAACGCCAGAAG+TGG 0.583659 3_4:-66168056 Msa0515150:CDS
ACATATGAAGATACATGAGA+TGG 0.585374 3_4:+66168454 None:intergenic
TAGTGTTGGAGCTAAGGCAA+AGG 0.590191 3_4:-66168207 Msa0515150:CDS
GGCTCCAAACTAATTGGGAA+AGG 0.604038 3_4:-66168175 Msa0515150:CDS
AGTCGAAGTGATTACAAAGA+GGG 0.604284 3_4:-66168079 Msa0515150:CDS
GAGTCGAAGTGATTACAAAG+AGG 0.657308 3_4:-66168080 Msa0515150:CDS
CAGCGCAGCACCAAGCACGA+TGG 0.681591 3_4:+66167930 None:intergenic
CTAAGGCAAAGGAAGATATG+GGG 0.687073 3_4:-66168196 Msa0515150:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATTAAAATAAAAGTAGCTT+CGG - chr3_4:66168317-66168336 Msa0515150:CDS 20.0%
! AAAACATGGAGCTAAAAAAA+TGG - chr3_4:66168223-66168242 Msa0515150:CDS 25.0%
! ATGAATAATTTGAATGAGTG+TGG - chr3_4:66168372-66168391 Msa0515150:CDS 25.0%
ACATATGAAGATACATGAGA+TGG + chr3_4:66167892-66167911 None:intergenic 30.0%
!!! ATTCTTTTGTTGTTCTCTGT+TGG + chr3_4:66168003-66168022 None:intergenic 30.0%
! AAGGTTTTCCATGAAATCTT+GGG + chr3_4:66168103-66168122 None:intergenic 30.0%
! TAAGGTTTTCCATGAAATCT+TGG + chr3_4:66168104-66168123 None:intergenic 30.0%
GATTCTTGAATCACAAAACA+TGG - chr3_4:66168209-66168228 Msa0515150:CDS 30.0%
GCTAAAAAAATGGTTGAAGA+TGG - chr3_4:66168233-66168252 Msa0515150:CDS 30.0%
! AAAATGGTTGAAGATGGATT+TGG - chr3_4:66168239-66168258 Msa0515150:CDS 30.0%
! AGGTTTTCCATGAAATCTTG+GGG + chr3_4:66168102-66168121 None:intergenic 35.0%
!! ACACTAGGCTTTGTTGATTA+AGG + chr3_4:66168122-66168141 None:intergenic 35.0%
TAATCAACAAAGCCTAGTGT+TGG - chr3_4:66168122-66168141 Msa0515150:CDS 35.0%
GATTCAAGAATCACTTTCCA+TGG + chr3_4:66168201-66168220 None:intergenic 35.0%
AGTCGAAGTGATTACAAAGA+GGG - chr3_4:66168264-66168283 Msa0515150:CDS 35.0%
AGATGGAGAAGCAAAATAAG+AGG - chr3_4:66168346-66168365 Msa0515150:CDS 35.0%
AATGAGTGTGGATGCAAAAA+CGG - chr3_4:66168384-66168403 Msa0515150:CDS 35.0%
GATTGTGATATATTTGCGCT+TGG - chr3_4:66168438-66168457 Msa0515150:CDS 35.0%
CTAAATGACTCGTCCCAAAA+GGG + chr3_4:66168063-66168082 None:intergenic 40.0%
ACTAAATGACTCGTCCCAAA+AGG + chr3_4:66168064-66168083 None:intergenic 40.0%
! GGTTTTCCATGAAATCTTGG+GGG + chr3_4:66168101-66168120 None:intergenic 40.0%
AGCTAAGGCAAAGGAAGATA+TGG - chr3_4:66168145-66168164 Msa0515150:CDS 40.0%
GCTAAGGCAAAGGAAGATAT+GGG - chr3_4:66168146-66168165 Msa0515150:CDS 40.0%
CTAAGGCAAAGGAAGATATG+GGG - chr3_4:66168147-66168166 Msa0515150:CDS 40.0%
ATATGGGGCTCCAAACTAAT+TGG - chr3_4:66168162-66168181 Msa0515150:CDS 40.0%
TATGGGGCTCCAAACTAATT+GGG - chr3_4:66168163-66168182 Msa0515150:CDS 40.0%
CTCACCTTTCCCAATTAGTT+TGG + chr3_4:66168175-66168194 None:intergenic 40.0%
GAGTCGAAGTGATTACAAAG+AGG - chr3_4:66168263-66168282 Msa0515150:CDS 40.0%
TATGTTCATAACGCCAGAAG+TGG - chr3_4:66168287-66168306 Msa0515150:CDS 40.0%
TTAATCTCCACTTCCACTTC+TGG + chr3_4:66168303-66168322 None:intergenic 40.0%
! CCAAAAGGGCAATGATTTGG+CGG + chr3_4:66168049-66168068 None:intergenic 45.0%
! CGCCAAATCATTGCCCTTTT+GGG - chr3_4:66168047-66168066 Msa0515150:CDS 45.0%
GTCCCAAAAGGGCAATGATT+TGG + chr3_4:66168052-66168071 None:intergenic 45.0%
TTCTTGCCCCCAAGATTTCA+TGG - chr3_4:66168092-66168111 Msa0515150:CDS 45.0%
! AAAGCCTAGTGTTGGAGCTA+AGG - chr3_4:66168130-66168149 Msa0515150:CDS 45.0%
TTTGCCTTAGCTCCAACACT+AGG + chr3_4:66168137-66168156 None:intergenic 45.0%
! TAGTGTTGGAGCTAAGGCAA+AGG - chr3_4:66168136-66168155 Msa0515150:CDS 45.0%
GGCTCCAAACTAATTGGGAA+AGG - chr3_4:66168168-66168187 Msa0515150:CDS 45.0%
TTGGGAAAGGTGAGATTCCA+TGG - chr3_4:66168181-66168200 Msa0515150:CDS 45.0%
! CCGCCAAATCATTGCCCTTT+TGG - chr3_4:66168046-66168065 Msa0515150:CDS 50.0%
CATAACGCCAGAAGTGGAAG+TGG - chr3_4:66168293-66168312 Msa0515150:CDS 50.0%
AGTAGCTTCGGTTGCTGAGA+TGG - chr3_4:66168329-66168348 Msa0515150:CDS 50.0%
AGCACCAAGCACGATGGTCA+TGG + chr3_4:66168410-66168429 None:intergenic 55.0%
ATTTGCGCTTGGCGCAGCTT+TGG - chr3_4:66168449-66168468 Msa0515150:CDS 55.0%
ACGGCCATGACCATCGTGCT+TGG - chr3_4:66168403-66168422 Msa0515150:CDS 60.0%
CAGCGCAGCACCAAGCACGA+TGG + chr3_4:66168416-66168435 None:intergenic 65.0%
! TGCGCTTGGCGCAGCTTTGG+AGG - chr3_4:66168452-66168471 Msa0515150:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 66167883 66168482 66167883 ID=Msa0515150;Name=Msa0515150
chr3_4 mRNA 66167883 66168482 66167883 ID=Msa0515150-mRNA-1;Parent=Msa0515150;Name=Msa0515150-mRNA-1;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|199
chr3_4 exon 66167883 66168482 66167883 ID=Msa0515150-mRNA-1:exon:17026;Parent=Msa0515150-mRNA-1
chr3_4 CDS 66167883 66168482 66167883 ID=Msa0515150-mRNA-1:cds;Parent=Msa0515150-mRNA-1
Gene Sequence

>Msa0515150

ATGGATCCATCTCATGTATCTTCATATGTAAACTTCAGCTGTGAATTGAGAATAATACAAGCTCGAAACATTGAATTCATCAAGACGACGAAAAACTTGTTTGCAAGATTGTATCTTCCAACAGAGAACAACAAAAGAATTCAACTCAACAGCAGAAATGTTTCCGCCAAATCATTGCCCTTTTGGGACGAGTCATTTAGTCTAGATTGTTCTTGCCCCCAAGATTTCATGGAAAACCTTAATCAACAAAGCCTAGTGTTGGAGCTAAGGCAAAGGAAGATATGGGGCTCCAAACTAATTGGGAAAGGTGAGATTCCATGGAAAGTGATTCTTGAATCACAAAACATGGAGCTAAAAAAATGGTTGAAGATGGATTTGGTGAGTCGAAGTGATTACAAAGAGGGTATGTTCATAACGCCAGAAGTGGAAGTGGAGATTAAAATAAAAGTAGCTTCGGTTGCTGAGATGGAGAAGCAAAATAAGAGGAGAATGAATAATTTGAATGAGTGTGGATGCAAAAACGGCCATGACCATCGTGCTTGGTGCTGCGCTGAAGATTGTGATATATTTGCGCTTGGCGCAGCTTTGGAGGCTTTTTAA

Protein sequence

>Msa0515150

MDPSHVSSYVNFSCELRIIQARNIEFIKTTKNLFARLYLPTENNKRIQLNSRNVSAKSLPFWDESFSLDCSCPQDFMENLNQQSLVLELRQRKIWGSKLIGKGEIPWKVILESQNMELKKWLKMDLVSRSDYKEGMFITPEVEVEIKIKVASVAEMEKQNKRRMNNLNECGCKNGHDHRAWCCAEDCDIFALGAALEAF*