AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1399430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1399430 MtrunA17_Chr6g0461591 92.308 273 5 3 1 269 1 261 0.0 507
Msa1399430 MtrunA17_Chr7g0253101 56.250 288 74 8 1 268 1 256 1.03e-98 290
Msa1399430 MtrunA17_Chr1g0146231 37.037 243 105 7 26 266 104 300 2.66e-36 132
Msa1399430 MtrunA17_Chr4g0035891 81.538 65 12 0 200 264 211 275 6.91e-34 124
Msa1399430 MtrunA17_Chr1g0199671 80.000 65 13 0 200 264 242 306 1.80e-33 124
Msa1399430 MtrunA17_Chr8g0372291 73.333 75 18 1 190 264 210 282 4.68e-33 122
Msa1399430 MtrunA17_Chr2g0326771 77.143 70 16 0 200 269 216 285 4.92e-33 122
Msa1399430 MtrunA17_Chr8g0340401 73.913 69 18 0 198 266 224 292 9.27e-33 122
Msa1399430 MtrunA17_Chr1g0213161 70.270 74 22 0 192 265 144 217 1.44e-32 119
Msa1399430 MtrunA17_Chr3g0140331 68.750 80 20 1 187 266 245 319 3.13e-32 121
Msa1399430 MtrunA17_Chr3g0145121 78.462 65 14 0 200 264 215 279 7.63e-32 119
Msa1399430 MtrunA17_Chr1g0169431 76.471 68 16 0 199 266 171 238 1.01e-31 117
Msa1399430 MtrunA17_Chr5g0404981 36.667 240 108 7 31 264 163 364 1.24e-31 120
Msa1399430 MtrunA17_Chr4g0028961 76.923 65 15 0 200 264 232 296 6.21e-31 117
Msa1399430 MtrunA17_Chr5g0395391 72.059 68 19 0 199 266 269 336 3.78e-30 115
Msa1399430 MtrunA17_Chr5g0395391 70.769 65 19 0 199 263 167 231 6.10e-28 109
Msa1399430 MtrunA17_Chr7g0232671 57.576 66 28 0 196 261 183 248 1.35e-20 88.2
Msa1399430 MtrunA17_Chr7g0232701 56.452 62 27 0 200 261 73 134 6.66e-19 80.9
Msa1399430 MtrunA17_Chr7g0232681 54.839 62 28 0 200 261 132 193 3.20e-18 80.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1399430 AT4G32890.1 37.546 269 106 10 2 266 53 263 4.81e-43 149
Msa1399430 AT2G45050.1 36.864 236 80 5 31 266 79 245 8.43e-37 131
Msa1399430 AT5G25830.1 34.444 270 128 9 2 266 60 285 1.38e-36 132
Msa1399430 AT3G60530.1 79.710 69 14 0 198 266 156 224 9.81e-36 128
Msa1399430 AT5G66320.2 34.909 275 128 7 2 264 78 313 2.44e-35 129
Msa1399430 AT5G66320.1 34.909 275 128 7 2 264 78 313 2.44e-35 129
Msa1399430 AT3G24050.1 75.000 68 17 0 199 266 193 260 8.74e-34 124
Msa1399430 AT4G36240.1 36.641 262 84 11 10 268 50 232 1.67e-32 119
Msa1399430 AT3G54810.1 67.470 83 26 1 182 264 212 293 1.80e-32 121
Msa1399430 AT3G54810.2 67.470 83 26 1 182 264 212 293 1.80e-32 121
Msa1399430 AT3G51080.1 81.538 65 12 0 200 264 221 285 2.94e-32 120
Msa1399430 AT1G08010.2 72.308 65 18 0 200 264 220 284 1.50e-31 119
Msa1399430 AT1G08010.1 72.308 65 18 0 200 264 220 284 1.50e-31 119
Msa1399430 AT1G08010.3 72.308 65 18 0 200 264 220 284 1.50e-31 119
Msa1399430 AT4G34680.1 73.846 65 17 0 200 264 180 244 1.80e-30 115
Msa1399430 AT4G34680.2 73.846 65 17 0 200 264 180 244 1.80e-30 115
Msa1399430 AT1G08000.2 72.727 66 18 0 199 264 217 282 3.12e-30 115
Msa1399430 AT1G08000.1 72.727 66 18 0 199 264 217 282 3.12e-30 115
Msa1399430 AT2G28340.1 66.154 65 22 0 202 266 193 257 1.20e-26 105
Msa1399430 AT3G45170.1 61.538 65 25 0 202 266 117 181 1.78e-25 100

Find 56 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGTTGAAACAAGCTTAT+TGG 0.158008 tig0022357:-70963 Msa1399430:CDS
TGCAACAATGTATCAGTTTC+AGG 0.183831 tig0022357:-72060 Msa1399430:CDS
CAGCTCCTAGTACCAAAATT+TGG 0.286985 tig0022357:-71032 Msa1399430:CDS
ATGTGAATGTGACCAAATTT+TGG 0.288556 tig0022357:+71020 None:intergenic
AATTCCTCTTAAAATTGATT+AGG 0.307837 tig0022357:+70424 None:intergenic
CATGCATTGCATAGTGTCTT+TGG 0.309772 tig0022357:+70667 None:intergenic
ATGGTCCTGCCCTCCATTGT+GGG 0.318440 tig0022357:+70695 None:intergenic
TTGAGCAATCATCAAAATAT+CGG 0.327672 tig0022357:+70316 None:intergenic
TCTCAAATAGCTTAACCTAT+TGG 0.330120 tig0022357:-70287 Msa1399430:three_prime_UTR
TGAGTGCACCTTCTTGCATT+TGG 0.340927 tig0022357:+70736 None:intergenic
CCGTGTTGTGAGCAGGAAAA+AGG 0.381223 tig0022357:+72016 None:intergenic
TAATTAGTGATTAGTGAAGT+TGG 0.399199 tig0022357:-70510 Msa1399430:three_prime_UTR
ACAATGGAGGGCAGGACCAT+TGG 0.400380 tig0022357:-70692 Msa1399430:CDS
AATGGTCCTGCCCTCCATTG+TGG 0.428822 tig0022357:+70694 None:intergenic
TTGCAAATATCCATGTCTAT+AGG 0.443264 tig0022357:+72078 None:intergenic
AGGGTGCGAAGAAGAGGAGG+AGG 0.446581 tig0022357:+72101 None:intergenic
TGAATTTGAACATTAGAAGA+AGG 0.457759 tig0022357:+71171 None:intergenic
GAGAATGCAATCTATTAAGT+AGG 0.465726 tig0022357:-70536 Msa1399430:exon
CAATGGAGGGCAGGACCATT+GGG 0.466961 tig0022357:-70691 Msa1399430:CDS
GGGTGCGAAGAAGAGGAGGA+GGG 0.484084 tig0022357:+72102 None:intergenic
AATTGATAGATGATGTTGAT+TGG 0.485292 tig0022357:-72293 Msa1399430:five_prime_UTR
CGGAGCAATGAGTTCGCTGT+CGG 0.489222 tig0022357:+70936 None:intergenic
GTAGGTTGTTGCCAGAGTAT+AGG 0.492955 tig0022357:-70624 Msa1399430:CDS
TTCGGAAGTTACGATCCAAT+AGG 0.494984 tig0022357:+70272 None:intergenic
AGACTAACCGTGTTGTGAGC+AGG 0.496025 tig0022357:+72009 None:intergenic
CATAGTGTCTTTGGTCCCAA+TGG 0.499888 tig0022357:+70676 None:intergenic
TGAGCAATCATCAAAATATC+GGG 0.504949 tig0022357:+70317 None:intergenic
TATAGGAGGGTGCGAAGAAG+AGG 0.524978 tig0022357:+72095 None:intergenic
CAAATATCCATGTCTATAGG+AGG 0.525607 tig0022357:+72081 None:intergenic
TCAGGAAGTGGATTTAGGGA+TGG 0.532369 tig0022357:-71253 Msa1399430:intron
TGCAAGTAGCTAACAAAAGT+AGG 0.538933 tig0022357:+70589 None:intergenic
AGGACCCCACAATGGAGGGC+AGG 0.548944 tig0022357:-70700 Msa1399430:CDS
TGGTCCTGCCCTCCATTGTG+GGG 0.559124 tig0022357:+70696 None:intergenic
AACATATTCCAAATGCAAGA+AGG 0.563153 tig0022357:-70744 Msa1399430:CDS
AAGTGGATTTAGGGATGGAG+TGG 0.569403 tig0022357:-71248 Msa1399430:CDS
AAGCTTGTTTCAACAAAGGA+GGG 0.570788 tig0022357:+70968 None:intergenic
AGAATGCAATCTATTAAGTA+GGG 0.572327 tig0022357:-70535 Msa1399430:exon
GAAGGTGCTATGACACAACT+TGG 0.573345 tig0022357:+71189 None:intergenic
TAAGCTTGTTTCAACAAAGG+AGG 0.573541 tig0022357:+70967 None:intergenic
ATGGTACTCAGAGCTTCTGT+CGG 0.579340 tig0022357:+72187 None:intergenic
AGGAGGGTGCGAAGAAGAGG+AGG 0.582183 tig0022357:+72098 None:intergenic
CATTCACATCTTATAAGTGA+TGG 0.584037 tig0022357:-71006 Msa1399430:CDS
TGTGGTGTGAGGTACAAGTC+TGG 0.585109 tig0022357:-70646 Msa1399430:CDS
CAATAAGCTTGTTTCAACAA+AGG 0.588114 tig0022357:+70964 None:intergenic
ACAAAGGACCCCACAATGGA+GGG 0.595325 tig0022357:-70704 Msa1399430:CDS
GTGTGAGGTACAAGTCTGGT+AGG 0.597780 tig0022357:-70642 Msa1399430:CDS
GCACTCATTGTTTGTCACAA+AGG 0.603068 tig0022357:-70720 Msa1399430:CDS
CACAAAGGACCCCACAATGG+AGG 0.605327 tig0022357:-70705 Msa1399430:CDS
ACAAAAGTAGGACTCTTCGC+TGG 0.624466 tig0022357:+70601 None:intergenic
TTCGCACCCTCCTATAGACA+TGG 0.629163 tig0022357:-72088 Msa1399430:exon
AAAGTCTCTGCAGTTGCGTG+CGG 0.637759 tig0022357:-70901 Msa1399430:CDS
TGATGTTAATGATGATGATG+AGG 0.646926 tig0022357:-70767 Msa1399430:CDS
AAATATCCATGTCTATAGGA+GGG 0.653251 tig0022357:+72082 None:intergenic
TGTCACAAAGGACCCCACAA+TGG 0.681006 tig0022357:-70708 Msa1399430:CDS
TATGCAATGCATGTGGTGTG+AGG 0.734829 tig0022357:-70657 Msa1399430:CDS
AAGACACTATGCAATGCATG+TGG 0.764250 tig0022357:-70664 Msa1399430:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAAAAAAGTTAATTAAAT+TGG + tig0022357:70371-70390 None:intergenic 10.0%
!! ATGAAAAAAGTTAATTAAAT+TGG + tig0022357:70371-70390 None:intergenic 10.0%
!!! CTAAATTTTGATTTGATTTT+TGG - tig0022357:70834-70853 Msa1399430:CDS 15.0%
!!! TACTTTTTTTTTTTTTGAAG+AGG - tig0022357:70937-70956 Msa1399430:CDS 15.0%
!!! ATAGAATTTGGTAATATTTA+AGG - tig0022357:71226-71245 Msa1399430:CDS 15.0%
!!! TAATAAATTTTGGTTTTTTG+TGG - tig0022357:71317-71336 Msa1399430:intron 15.0%
!!! CTAAATTTTGATTTGATTTT+TGG - tig0022357:70834-70853 Msa1399430:CDS 15.0%
!!! TACTTTTTTTTTTTTTGAAG+AGG - tig0022357:70937-70956 Msa1399430:CDS 15.0%
!!! ATAGAATTTGGTAATATTTA+AGG - tig0022357:71226-71245 Msa1399430:CDS 15.0%
!!! TAATAAATTTTGGTTTTTTG+TGG - tig0022357:71317-71336 Msa1399430:intron 15.0%
!!! TGTAATACTTTAAGTTCATA+AGG - tig0022357:70723-70742 Msa1399430:CDS 20.0%
!!! AGTATGTAGCTAATAAATTT+TGG - tig0022357:71307-71326 Msa1399430:intron 20.0%
!! AATTCCTCTTAAAATTGATT+AGG + tig0022357:72180-72199 None:intergenic 20.0%
!!! TTTTAAGAGGAATTAAATCA+TGG - tig0022357:72186-72205 Msa1399430:five_prime_UTR 20.0%
!!! TGTAATACTTTAAGTTCATA+AGG - tig0022357:70723-70742 Msa1399430:CDS 20.0%
!!! AGTATGTAGCTAATAAATTT+TGG - tig0022357:71307-71326 Msa1399430:intron 20.0%
!! AATTCCTCTTAAAATTGATT+AGG + tig0022357:72180-72199 None:intergenic 20.0%
!!! TTTTAAGAGGAATTAAATCA+TGG - tig0022357:72186-72205 Msa1399430:five_prime_UTR 20.0%
! AATTGATAGATGATGTTGAT+TGG - tig0022357:70308-70327 Msa1399430:three_prime_UTR 25.0%
! TTGAAGAGGCTAAAATAATT+TGG - tig0022357:70951-70970 Msa1399430:CDS 25.0%
!!! TCAAGTGTTCTATTCTTTTT+TGG - tig0022357:71104-71123 Msa1399430:CDS 25.0%
! ATTTGTGTATGCATAGAATT+TGG - tig0022357:71214-71233 Msa1399430:CDS 25.0%
!!! TTTTTTGTGGCAAATTTTTC+AGG - tig0022357:71330-71349 Msa1399430:intron 25.0%
! TGAATTTGAACATTAGAAGA+AGG + tig0022357:71433-71452 None:intergenic 25.0%
!!! TAAGATTGATTTTGTTGTTG+AGG + tig0022357:71496-71515 None:intergenic 25.0%
! AGAATGCAATCTATTAAGTA+GGG - tig0022357:72066-72085 Msa1399430:CDS 25.0%
! TAATTAGTGATTAGTGAAGT+TGG - tig0022357:72091-72110 Msa1399430:exon 25.0%
!! AGTTCCTAATCAATTTTAAG+AGG - tig0022357:72173-72192 Msa1399430:five_prime_UTR 25.0%
!! TAGAGTTACTGATATTTTTC+TGG - tig0022357:72254-72273 Msa1399430:five_prime_UTR 25.0%
! TTGAGCAATCATCAAAATAT+CGG + tig0022357:72288-72307 None:intergenic 25.0%
! AATTGATAGATGATGTTGAT+TGG - tig0022357:70308-70327 Msa1399430:three_prime_UTR 25.0%
! TTGAAGAGGCTAAAATAATT+TGG - tig0022357:70951-70970 Msa1399430:CDS 25.0%
!!! TCAAGTGTTCTATTCTTTTT+TGG - tig0022357:71104-71123 Msa1399430:CDS 25.0%
! ATTTGTGTATGCATAGAATT+TGG - tig0022357:71214-71233 Msa1399430:CDS 25.0%
!!! TTTTTTGTGGCAAATTTTTC+AGG - tig0022357:71330-71349 Msa1399430:intron 25.0%
! TGAATTTGAACATTAGAAGA+AGG + tig0022357:71433-71452 None:intergenic 25.0%
!!! TAAGATTGATTTTGTTGTTG+AGG + tig0022357:71496-71515 None:intergenic 25.0%
! AGAATGCAATCTATTAAGTA+GGG - tig0022357:72066-72085 Msa1399430:CDS 25.0%
! TAATTAGTGATTAGTGAAGT+TGG - tig0022357:72091-72110 Msa1399430:exon 25.0%
!! AGTTCCTAATCAATTTTAAG+AGG - tig0022357:72173-72192 Msa1399430:five_prime_UTR 25.0%
!! TAGAGTTACTGATATTTTTC+TGG - tig0022357:72254-72273 Msa1399430:five_prime_UTR 25.0%
! TTGAGCAATCATCAAAATAT+CGG + tig0022357:72288-72307 None:intergenic 25.0%
! ATTCTGAGTGTTCTCTTTTA+TGG + tig0022357:70436-70455 None:intergenic 30.0%
AAAAAGAACAGAAGCAAGTT+TGG + tig0022357:70475-70494 None:intergenic 30.0%
AAATATCCATGTCTATAGGA+GGG + tig0022357:70522-70541 None:intergenic 30.0%
TTGCAAATATCCATGTCTAT+AGG + tig0022357:70526-70545 None:intergenic 30.0%
GCTAGTACTTTATGCAAAAA+TGG - tig0022357:70667-70686 Msa1399430:CDS 30.0%
GTTTGTGTATGCATAGAATT+TGG - tig0022357:70911-70930 Msa1399430:CDS 30.0%
!!! ATTTTTCAGGAAGTGGATTT+AGG - tig0022357:71343-71362 Msa1399430:intron 30.0%
!!! TTTTTCAGGAAGTGGATTTA+GGG - tig0022357:71344-71363 Msa1399430:intron 30.0%
!!! TTGCAAAATTTTGTTGTTCC+TGG - tig0022357:71517-71536 Msa1399430:intron 30.0%
! ATGTGAATGTGACCAAATTT+TGG + tig0022357:71584-71603 None:intergenic 30.0%
CATTCACATCTTATAAGTGA+TGG - tig0022357:71595-71614 Msa1399430:intron 30.0%
! CAATAAGCTTGTTTCAACAA+AGG + tig0022357:71640-71659 None:intergenic 30.0%
CTTTGTTGAAACAAGCTTAT+TGG - tig0022357:71638-71657 Msa1399430:intron 30.0%
!! GAGACTTTTTGCTCATTTTT+CGG + tig0022357:71688-71707 None:intergenic 30.0%
! TGATGTTAATGATGATGATG+AGG - tig0022357:71834-71853 Msa1399430:intron 30.0%
AACATATTCCAAATGCAAGA+AGG - tig0022357:71857-71876 Msa1399430:intron 30.0%
GAGAATGCAATCTATTAAGT+AGG - tig0022357:72065-72084 Msa1399430:CDS 30.0%
TGAGCAATCATCAAAATATC+GGG + tig0022357:72287-72306 None:intergenic 30.0%
TCTCAAATAGCTTAACCTAT+TGG - tig0022357:72314-72333 Msa1399430:five_prime_UTR 30.0%
!!! TCCAACTACATACCTTTTTT+CGG + tig0022357:70274-70293 None:intergenic 30.0%
! ATTCTGAGTGTTCTCTTTTA+TGG + tig0022357:70436-70455 None:intergenic 30.0%
AAAAAGAACAGAAGCAAGTT+TGG + tig0022357:70475-70494 None:intergenic 30.0%
AAATATCCATGTCTATAGGA+GGG + tig0022357:70522-70541 None:intergenic 30.0%
TTGCAAATATCCATGTCTAT+AGG + tig0022357:70526-70545 None:intergenic 30.0%
GCTAGTACTTTATGCAAAAA+TGG - tig0022357:70667-70686 Msa1399430:CDS 30.0%
GTTTGTGTATGCATAGAATT+TGG - tig0022357:70911-70930 Msa1399430:CDS 30.0%
!!! ATTTTTCAGGAAGTGGATTT+AGG - tig0022357:71343-71362 Msa1399430:intron 30.0%
!!! TTTTTCAGGAAGTGGATTTA+GGG - tig0022357:71344-71363 Msa1399430:intron 30.0%
!!! TTGCAAAATTTTGTTGTTCC+TGG - tig0022357:71517-71536 Msa1399430:intron 30.0%
! ATGTGAATGTGACCAAATTT+TGG + tig0022357:71584-71603 None:intergenic 30.0%
CATTCACATCTTATAAGTGA+TGG - tig0022357:71595-71614 Msa1399430:intron 30.0%
! CAATAAGCTTGTTTCAACAA+AGG + tig0022357:71640-71659 None:intergenic 30.0%
CTTTGTTGAAACAAGCTTAT+TGG - tig0022357:71638-71657 Msa1399430:intron 30.0%
!! GAGACTTTTTGCTCATTTTT+CGG + tig0022357:71688-71707 None:intergenic 30.0%
! TGATGTTAATGATGATGATG+AGG - tig0022357:71834-71853 Msa1399430:intron 30.0%
AACATATTCCAAATGCAAGA+AGG - tig0022357:71857-71876 Msa1399430:intron 30.0%
GAGAATGCAATCTATTAAGT+AGG - tig0022357:72065-72084 Msa1399430:CDS 30.0%
TGAGCAATCATCAAAATATC+GGG + tig0022357:72287-72306 None:intergenic 30.0%
TCTCAAATAGCTTAACCTAT+TGG - tig0022357:72314-72333 Msa1399430:five_prime_UTR 30.0%
CAAATATCCATGTCTATAGG+AGG + tig0022357:70523-70542 None:intergenic 35.0%
TGCAACAATGTATCAGTTTC+AGG - tig0022357:70541-70560 Msa1399430:CDS 35.0%
! GGTTTTTGCATTGTGTTTGA+AGG + tig0022357:71475-71494 None:intergenic 35.0%
!! TGTGACCAAATTTTGGTACT+AGG + tig0022357:71577-71596 None:intergenic 35.0%
! AAGCTTGTTTCAACAAAGGA+GGG + tig0022357:71636-71655 None:intergenic 35.0%
! TAAGCTTGTTTCAACAAAGG+AGG + tig0022357:71637-71656 None:intergenic 35.0%
ATCAGACAGAAGCAAAAAGA+AGG - tig0022357:71725-71744 Msa1399430:intron 35.0%
TCAGACAGAAGCAAAAAGAA+GGG - tig0022357:71726-71745 Msa1399430:intron 35.0%
! GAAAGAGAAAGAGAGTTTTG+AGG - tig0022357:71771-71790 Msa1399430:intron 35.0%
TGCAAGTAGCTAACAAAAGT+AGG + tig0022357:72015-72034 None:intergenic 35.0%
CAAATATCCATGTCTATAGG+AGG + tig0022357:70523-70542 None:intergenic 35.0%
TGCAACAATGTATCAGTTTC+AGG - tig0022357:70541-70560 Msa1399430:CDS 35.0%
! GGTTTTTGCATTGTGTTTGA+AGG + tig0022357:71475-71494 None:intergenic 35.0%
!! TGTGACCAAATTTTGGTACT+AGG + tig0022357:71577-71596 None:intergenic 35.0%
! AAGCTTGTTTCAACAAAGGA+GGG + tig0022357:71636-71655 None:intergenic 35.0%
! TAAGCTTGTTTCAACAAAGG+AGG + tig0022357:71637-71656 None:intergenic 35.0%
ATCAGACAGAAGCAAAAAGA+AGG - tig0022357:71725-71744 Msa1399430:intron 35.0%
TCAGACAGAAGCAAAAAGAA+GGG - tig0022357:71726-71745 Msa1399430:intron 35.0%
! GAAAGAGAAAGAGAGTTTTG+AGG - tig0022357:71771-71790 Msa1399430:intron 35.0%
TGCAAGTAGCTAACAAAAGT+AGG + tig0022357:72015-72034 None:intergenic 35.0%
AGGAAAAAGGTCATCAATGG+TGG + tig0022357:70575-70594 None:intergenic 40.0%
AGCAGGAAAAAGGTCATCAA+TGG + tig0022357:70578-70597 None:intergenic 40.0%
!!! GTGGCAAATTTTTCAGGAAG+TGG - tig0022357:71336-71355 Msa1399430:intron 40.0%
CAGCTCCTAGTACCAAAATT+TGG - tig0022357:71569-71588 Msa1399430:intron 40.0%
AAAAAGAAGGGTGAAGAGAG+TGG - tig0022357:71738-71757 Msa1399430:intron 40.0%
GGTGAAGAGAGTGGTAAAAA+TGG - tig0022357:71747-71766 Msa1399430:intron 40.0%
!!! GAGAAAGAGAGTTTTGAGGT+TGG - tig0022357:71775-71794 Msa1399430:intron 40.0%
GCACTCATTGTTTGTCACAA+AGG - tig0022357:71881-71900 Msa1399430:intron 40.0%
CATGCATTGCATAGTGTCTT+TGG + tig0022357:71937-71956 None:intergenic 40.0%
AAGACACTATGCAATGCATG+TGG - tig0022357:71937-71956 Msa1399430:intron 40.0%
AGGAAAAAGGTCATCAATGG+TGG + tig0022357:70575-70594 None:intergenic 40.0%
AGCAGGAAAAAGGTCATCAA+TGG + tig0022357:70578-70597 None:intergenic 40.0%
!!! GTGGCAAATTTTTCAGGAAG+TGG - tig0022357:71336-71355 Msa1399430:intron 40.0%
CAGCTCCTAGTACCAAAATT+TGG - tig0022357:71569-71588 Msa1399430:intron 40.0%
AAAAAGAAGGGTGAAGAGAG+TGG - tig0022357:71738-71757 Msa1399430:intron 40.0%
GGTGAAGAGAGTGGTAAAAA+TGG - tig0022357:71747-71766 Msa1399430:intron 40.0%
!!! GAGAAAGAGAGTTTTGAGGT+TGG - tig0022357:71775-71794 Msa1399430:intron 40.0%
GCACTCATTGTTTGTCACAA+AGG - tig0022357:71881-71900 Msa1399430:intron 40.0%
CATGCATTGCATAGTGTCTT+TGG + tig0022357:71937-71956 None:intergenic 40.0%
AAGACACTATGCAATGCATG+TGG - tig0022357:71937-71956 Msa1399430:intron 40.0%
ATGGTACTCAGAGCTTCTGT+CGG + tig0022357:70417-70436 None:intergenic 45.0%
! CCTTTTTCCTGCTCACAACA+CGG - tig0022357:70585-70604 Msa1399430:CDS 45.0%
! TCAGGAAGTGGATTTAGGGA+TGG - tig0022357:71348-71367 Msa1399430:intron 45.0%
! AAGTGGATTTAGGGATGGAG+TGG - tig0022357:71353-71372 Msa1399430:intron 45.0%
!! GAAGGTGCTATGACACAACT+TGG + tig0022357:71415-71434 None:intergenic 45.0%
!! CTCTTGCTTCTTGCTTTTCC+AGG + tig0022357:71538-71557 None:intergenic 45.0%
TGAGTGCACCTTCTTGCATT+TGG + tig0022357:71868-71887 None:intergenic 45.0%
CATAGTGTCTTTGGTCCCAA+TGG + tig0022357:71928-71947 None:intergenic 45.0%
TATGCAATGCATGTGGTGTG+AGG - tig0022357:71944-71963 Msa1399430:intron 45.0%
GTAGGTTGTTGCCAGAGTAT+AGG - tig0022357:71977-71996 Msa1399430:intron 45.0%
ACAAAAGTAGGACTCTTCGC+TGG + tig0022357:72003-72022 None:intergenic 45.0%
ATGGTACTCAGAGCTTCTGT+CGG + tig0022357:70417-70436 None:intergenic 45.0%
! CCTTTTTCCTGCTCACAACA+CGG - tig0022357:70585-70604 Msa1399430:CDS 45.0%
! TCAGGAAGTGGATTTAGGGA+TGG - tig0022357:71348-71367 Msa1399430:intron 45.0%
! AAGTGGATTTAGGGATGGAG+TGG - tig0022357:71353-71372 Msa1399430:intron 45.0%
!! GAAGGTGCTATGACACAACT+TGG + tig0022357:71415-71434 None:intergenic 45.0%
!! CTCTTGCTTCTTGCTTTTCC+AGG + tig0022357:71538-71557 None:intergenic 45.0%
TGAGTGCACCTTCTTGCATT+TGG + tig0022357:71868-71887 None:intergenic 45.0%
CATAGTGTCTTTGGTCCCAA+TGG + tig0022357:71928-71947 None:intergenic 45.0%
TATGCAATGCATGTGGTGTG+AGG - tig0022357:71944-71963 Msa1399430:intron 45.0%
GTAGGTTGTTGCCAGAGTAT+AGG - tig0022357:71977-71996 Msa1399430:intron 45.0%
ACAAAAGTAGGACTCTTCGC+TGG + tig0022357:72003-72022 None:intergenic 45.0%
!!! AAATTTTGATTATATTTTTT+TGG - tig0022357:71131-71150 Msa1399430:CDS 5.0%
!!! AAATTTTGATTATATTTTTT+TGG - tig0022357:71131-71150 Msa1399430:CDS 5.0%
! TATAGGAGGGTGCGAAGAAG+AGG + tig0022357:70509-70528 None:intergenic 50.0%
TTCGCACCCTCCTATAGACA+TGG - tig0022357:70513-70532 Msa1399430:three_prime_UTR 50.0%
CCGTGTTGTGAGCAGGAAAA+AGG + tig0022357:70588-70607 None:intergenic 50.0%
AGACTAACCGTGTTGTGAGC+AGG + tig0022357:70595-70614 None:intergenic 50.0%
AAAGTCTCTGCAGTTGCGTG+CGG - tig0022357:71700-71719 Msa1399430:intron 50.0%
TGTCACAAAGGACCCCACAA+TGG - tig0022357:71893-71912 Msa1399430:intron 50.0%
ACAAAGGACCCCACAATGGA+GGG - tig0022357:71897-71916 Msa1399430:intron 50.0%
TGTGGTGTGAGGTACAAGTC+TGG - tig0022357:71955-71974 Msa1399430:intron 50.0%
GTGTGAGGTACAAGTCTGGT+AGG - tig0022357:71959-71978 Msa1399430:intron 50.0%
! TATAGGAGGGTGCGAAGAAG+AGG + tig0022357:70509-70528 None:intergenic 50.0%
TTCGCACCCTCCTATAGACA+TGG - tig0022357:70513-70532 Msa1399430:three_prime_UTR 50.0%
CCGTGTTGTGAGCAGGAAAA+AGG + tig0022357:70588-70607 None:intergenic 50.0%
AGACTAACCGTGTTGTGAGC+AGG + tig0022357:70595-70614 None:intergenic 50.0%
AAAGTCTCTGCAGTTGCGTG+CGG - tig0022357:71700-71719 Msa1399430:intron 50.0%
TGTCACAAAGGACCCCACAA+TGG - tig0022357:71893-71912 Msa1399430:intron 50.0%
ACAAAGGACCCCACAATGGA+GGG - tig0022357:71897-71916 Msa1399430:intron 50.0%
TGTGGTGTGAGGTACAAGTC+TGG - tig0022357:71955-71974 Msa1399430:intron 50.0%
GTGTGAGGTACAAGTCTGGT+AGG - tig0022357:71959-71978 Msa1399430:intron 50.0%
CGGAGCAATGAGTTCGCTGT+CGG + tig0022357:71668-71687 None:intergenic 55.0%
CACAAAGGACCCCACAATGG+AGG - tig0022357:71896-71915 Msa1399430:intron 55.0%
ATGGTCCTGCCCTCCATTGT+GGG + tig0022357:71909-71928 None:intergenic 55.0%
AATGGTCCTGCCCTCCATTG+TGG + tig0022357:71910-71929 None:intergenic 55.0%
ACAATGGAGGGCAGGACCAT+TGG - tig0022357:71909-71928 Msa1399430:intron 55.0%
CAATGGAGGGCAGGACCATT+GGG - tig0022357:71910-71929 Msa1399430:intron 55.0%
! CTCTTCGCTGGCCTATACTC+TGG + tig0022357:71991-72010 None:intergenic 55.0%
CGGAGCAATGAGTTCGCTGT+CGG + tig0022357:71668-71687 None:intergenic 55.0%
CACAAAGGACCCCACAATGG+AGG - tig0022357:71896-71915 Msa1399430:intron 55.0%
ATGGTCCTGCCCTCCATTGT+GGG + tig0022357:71909-71928 None:intergenic 55.0%
AATGGTCCTGCCCTCCATTG+TGG + tig0022357:71910-71929 None:intergenic 55.0%
ACAATGGAGGGCAGGACCAT+TGG - tig0022357:71909-71928 Msa1399430:intron 55.0%
CAATGGAGGGCAGGACCATT+GGG - tig0022357:71910-71929 Msa1399430:intron 55.0%
! CTCTTCGCTGGCCTATACTC+TGG + tig0022357:71991-72010 None:intergenic 55.0%
GGGTGCGAAGAAGAGGAGGA+GGG + tig0022357:70502-70521 None:intergenic 60.0%
AGGGTGCGAAGAAGAGGAGG+AGG + tig0022357:70503-70522 None:intergenic 60.0%
! AGGAGGGTGCGAAGAAGAGG+AGG + tig0022357:70506-70525 None:intergenic 60.0%
TGGTCCTGCCCTCCATTGTG+GGG + tig0022357:71908-71927 None:intergenic 60.0%
GGGTGCGAAGAAGAGGAGGA+GGG + tig0022357:70502-70521 None:intergenic 60.0%
AGGGTGCGAAGAAGAGGAGG+AGG + tig0022357:70503-70522 None:intergenic 60.0%
! AGGAGGGTGCGAAGAAGAGG+AGG + tig0022357:70506-70525 None:intergenic 60.0%
TGGTCCTGCCCTCCATTGTG+GGG + tig0022357:71908-71927 None:intergenic 60.0%
AGGACCCCACAATGGAGGGC+AGG - tig0022357:71901-71920 Msa1399430:intron 65.0%
AGGACCCCACAATGGAGGGC+AGG - tig0022357:71901-71920 Msa1399430:intron 65.0%
Chromosome Type Strat End Strand Name
tig0022357 gene 70274 72349 70274 ID=Msa1399430;Name=Msa1399430
tig0022357 mRNA 70274 72349 70274 ID=Msa1399430-mRNA-1;Parent=Msa1399430;Name=Msa1399430-mRNA-1;_AED=0.00;_eAED=0.00;_QI=258|1|1|1|1|1|2|263|269
tig0022357 exon 70274 71271 70274 ID=Msa1399430-mRNA-1:exon:17186;Parent=Msa1399430-mRNA-1
tig0022357 exon 72017 72349 72017 ID=Msa1399430-mRNA-1:exon:17185;Parent=Msa1399430-mRNA-1
tig0022357 five_prime_UTR 72092 72349 72092 ID=Msa1399430-mRNA-1:five_prime_utr;Parent=Msa1399430-mRNA-1
tig0022357 CDS 72017 72091 72017 ID=Msa1399430-mRNA-1:cds;Parent=Msa1399430-mRNA-1
tig0022357 CDS 70537 71271 70537 ID=Msa1399430-mRNA-1:cds;Parent=Msa1399430-mRNA-1
tig0022357 three_prime_UTR 70274 70536 70274 ID=Msa1399430-mRNA-1:three_prime_utr;Parent=Msa1399430-mRNA-1
Gene Sequence

>Msa1399430

ATGGATATTTGCAACAATGTATCAGTTTCAGGAGAGTTTTCCACCATTGATGACCTTTTTCCTGCTCACAACACGGAAGTGGATTTAGGGATGGAGTGGTTGTCAGTGTTTGTTGAAGAATGTTTTTCAAGTAAACCAAGTTGTGTCATAGCACCTTCTTCTAATGTTCAAATTCAAGAAAGTACAAACACAAAACCTTCAAACACAATGCAAAAACCTCAACAACAAAATCAATCTTATTTGCAAAATTTTGTTGTTCCTGGAAAAGCAAGAAGCAAGAGAAAAAGACTTTCAGCTCCTAGTACCAAAATTTGGTCACATTCACATCTTATAAGTGATGGTAATTTGATTTCTGACCCTCCTTTGTTGAAACAAGCTTATTGGTTAGCCGACAGCGAACTCATTGCTCCGAAAAATGAGCAAAAAGTCTCTGCAGTTGCGTGCGGAGATCAGACAGAAGCAAAAAGAAGGGTGAAGAGAGTGGTAAAAATGGAGAAAGAGAAAGAGAGTTTTGAGGTTGGAATTATTCAAGTTAAGAATAGTGAGAATAATAATGATGATGTTAATGATGATGATGAGGAACATATTCCAAATGCAAGAAGGTGCACTCATTGTTTGTCACAAAGGACCCCACAATGGAGGGCAGGACCATTGGGACCAAAGACACTATGCAATGCATGTGGTGTGAGGTACAAGTCTGGTAGGTTGTTGCCAGAGTATAGGCCAGCGAAGAGTCCTACTTTTGTTAGCTACTTGCATTCAAATTCACACAAGAAAGTTCTTGAGATGAGAATGCAATCTATTAAGTAG
Protein sequence

>Msa1399430

MDICNNVSVSGEFSTIDDLFPAHNTEVDLGMEWLSVFVEECFSSKPSCVIAPSSNVQIQESTNTKPSNTMQKPQQQNQSYLQNFVVPGKARSKRKRLSAPSTKIWSHSHLISDGNLISDPPLLKQAYWLADSELIAPKNEQKVSAVACGDQTEAKRRVKRVVKMEKEKESFEVGIIQVKNSENNNDDVNDDDEEHIPNARRCTHCLSQRTPQWRAGPLGPKTLCNACGVRYKSGRLLPEYRPAKSPTFVSYLHSNSHKKVLEMRMQSIK*