AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0332280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0332280 MtrunA17_Chr2g0315201 98.312 237 4 0 1 237 80 316 5.29e-169 468
Msa0332280 MtrunA17_Chr2g0315211 68.033 244 71 1 1 237 80 323 5.03e-111 322
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0332280 AT1G78700.1 61.024 254 73 7 1 237 81 325 5.89e-83 251
Msa0332280 AT4G18890.2 52.049 244 71 10 1 237 4 208 3.09e-61 191
Msa0332280 AT4G18890.1 52.049 244 71 10 1 237 80 284 3.07e-60 191
Msa0332280 AT1G19350.3 33.692 279 98 13 2 235 118 354 4.16e-14 71.2
Msa0332280 AT1G19350.5 33.692 279 98 13 2 235 96 332 6.16e-14 70.5
Msa0332280 AT1G19350.1 33.692 279 98 13 2 235 96 332 6.16e-14 70.5
Msa0332280 AT1G19350.6 33.692 279 98 13 2 235 96 332 6.16e-14 70.5
Msa0332280 AT1G19350.4 33.692 279 98 13 2 235 96 332 6.16e-14 70.5

Find 87 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATCCCAGCAAACCATTC+AGG 0.319016 2_4:+49191610 None:intergenic
ATTATCATTATCATCATTGT+AGG 0.321157 2_4:-49191272 None:intergenic
TACTCCTTCCTGCCTTCTTC+CGG 0.325671 2_4:-49191664 Msa0332280:CDS
AAGGAAGACGAGCCGGGGCT+TGG 0.330367 2_4:+49191937 None:intergenic
TCGAAGCCCATTTGCCTTCA+AGG 0.331246 2_4:-49191542 Msa0332280:CDS
CATTTGGAAGCAACACATTT+GGG 0.336623 2_4:-49191387 Msa0332280:CDS
CTCTCCACAGCTTCATCTTC+AGG 0.340264 2_4:-49191838 Msa0332280:CDS
ATGGAGCCACTATGAAAATA+AGG 0.355143 2_4:+49191793 None:intergenic
AAATGCAAATTCATCTGAAA+TGG 0.358381 2_4:+49191405 None:intergenic
GCATTTGGAAGCAACACATT+TGG 0.360050 2_4:-49191388 Msa0332280:CDS
GCATCTTCCTTGAAGGCAAA+TGG 0.365893 2_4:+49191535 None:intergenic
ATGTGGACCCCTGGACAGTC+AGG 0.369643 2_4:-49191490 Msa0332280:CDS
GCCTTTAGAGAACATGGATA+TGG 0.373174 2_4:-49192001 None:intergenic
CTTGGGAAGGAAGACGAGCC+GGG 0.389874 2_4:+49191931 None:intergenic
TCTGCACGGCCCGGGTGGAC+TGG 0.392696 2_4:-49191694 Msa0332280:CDS
GTTGATCCTGAATGGTTTGC+TGG 0.393927 2_4:-49191616 Msa0332280:CDS
ACTATCTGACGGCCAGGGCT+TGG 0.399313 2_4:+49191637 None:intergenic
ATTTCAGATGAATTTGCATT+TGG 0.399618 2_4:-49191403 Msa0332280:CDS
GAGCTCAGGGGAGGTGTGAC+AGG 0.403615 2_4:+49191763 None:intergenic
CATCTGCTACAAATTCTTCA+TGG 0.405598 2_4:+49191335 None:intergenic
AAATAAGGATGAGGAAGTTT+CGG 0.409483 2_4:+49191808 None:intergenic
CATTTGGGATTGTGAAGCCT+TGG 0.410546 2_4:-49191372 Msa0332280:CDS
ACGGCCAGGGCTTGGAGGAC+CGG 0.427775 2_4:+49191645 None:intergenic
TCAAAGCCTGCTGCAATAGC+TGG 0.436153 2_4:+49191457 None:intergenic
GAACATGCCCCTGACTGTCC+AGG 0.438349 2_4:+49191481 None:intergenic
TTCTAGCAGTCGGAGAGCTC+AGG 0.440476 2_4:+49191749 None:intergenic
CATCTTCCTTGAAGGCAAAT+GGG 0.441986 2_4:+49191536 None:intergenic
TCTGAAATGGCTTCAGACAT+TGG 0.460873 2_4:+49191418 None:intergenic
AGATTGAATGTCGGAGAAGT+TGG 0.467632 2_4:+49191571 None:intergenic
TGTTCTCCAGCTATTGCAGC+AGG 0.468934 2_4:-49191463 Msa0332280:CDS
GGGCTTGGAGGACCGGAAGA+AGG 0.476639 2_4:+49191652 None:intergenic
TGGATCCTTTCTCCTTCCCA+AGG 0.477640 2_4:+49191355 None:intergenic
CAGGCAGCGGTTCGCGCATG+TGG 0.479649 2_4:-49191507 Msa0332280:CDS
GCGTTACGATTTGCAGCGTA+AGG 0.480138 2_4:+49191895 None:intergenic
ATGATCAATCTGCACGGCCC+GGG 0.481435 2_4:-49191702 Msa0332280:CDS
ATCTGACGGCCAGGGCTTGG+AGG 0.481702 2_4:+49191640 None:intergenic
GTCAGATAGTTGATCCTGAA+TGG 0.488622 2_4:-49191624 Msa0332280:CDS
TAAGGGGATGATGATGGACT+TGG 0.490391 2_4:+49191913 None:intergenic
ATTTGGGATTGTGAAGCCTT+GGG 0.491602 2_4:-49191371 Msa0332280:CDS
ACTTGGGAAGGAAGACGAGC+CGG 0.496049 2_4:+49191930 None:intergenic
TCTTCCGGTCCTCCAAGCCC+TGG 0.498896 2_4:-49191649 Msa0332280:CDS
GATGATCAATCTGCACGGCC+CGG 0.503778 2_4:-49191703 Msa0332280:CDS
TTGGAGGACCGGAAGAAGGC+AGG 0.505728 2_4:+49191656 None:intergenic
TGAAGCCTTGGGAAGGAGAA+AGG 0.506728 2_4:-49191360 Msa0332280:CDS
GGATCAACTATCTGACGGCC+AGG 0.508622 2_4:+49191631 None:intergenic
ATGGTAATTCTCTCATTCCA+TGG 0.516736 2_4:-49191870 Msa0332280:CDS
AGGTGTGACAGGAGCACTGA+TGG 0.518748 2_4:+49191774 None:intergenic
GAGAACATGGATATGGTTGG+TGG 0.530636 2_4:-49191994 None:intergenic
CGTTACGATTTGCAGCGTAA+GGG 0.534170 2_4:+49191896 None:intergenic
AAGACGAGCCGGGGCTTGGA+TGG 0.534291 2_4:+49191941 None:intergenic
GTTACGATTTGCAGCGTAAG+GGG 0.536907 2_4:+49191897 None:intergenic
GCTGCAAATCGTAACGCTGA+TGG 0.540162 2_4:-49191889 Msa0332280:CDS
TTGGGAAGGAAGACGAGCCG+GGG 0.553399 2_4:+49191932 None:intergenic
TCTAGCAGTCGGAGAGCTCA+GGG 0.554727 2_4:+49191750 None:intergenic
AACATGCCCCTGACTGTCCA+GGG 0.556233 2_4:+49191482 None:intergenic
CCACTATGAAAATAAGGATG+AGG 0.557357 2_4:+49191799 None:intergenic
GTAATGTTGTCCAGTCCACC+CGG 0.557358 2_4:+49191684 None:intergenic
GGTTCGCGCATGTGGACCCC+TGG 0.559214 2_4:-49191499 Msa0332280:CDS
GGATGATGATGGACTTGGGA+AGG 0.561521 2_4:+49191918 None:intergenic
TCATCTTACCATCCAAGCCC+CGG 0.562153 2_4:-49191949 Msa0332280:CDS
GCAGCGTAAGGGGATGATGA+TGG 0.565277 2_4:+49191907 None:intergenic
ATCAATCTGCACGGCCCGGG+TGG 0.570226 2_4:-49191699 Msa0332280:CDS
TTGATCCTGAATGGTTTGCT+GGG 0.572973 2_4:-49191615 Msa0332280:CDS
TGTGGACCCCTGGACAGTCA+GGG 0.577345 2_4:-49191489 Msa0332280:CDS
GGGATTGTGAAGCCTTGGGA+AGG 0.580106 2_4:-49191367 Msa0332280:CDS
TTAGAGAACATGGATATGGT+TGG 0.590409 2_4:-49191997 None:intergenic
AGGACCGGAAGAAGGCAGGA+AGG 0.591677 2_4:+49191660 None:intergenic
GTGGACCCCTGGACAGTCAG+GGG 0.607774 2_4:-49191488 Msa0332280:CDS
TGATAATGATAATAAGTACC+TGG 0.609283 2_4:+49191282 None:intergenic
CGAGATACAAGATTGAATGT+CGG 0.609297 2_4:+49191562 None:intergenic
CTTGGATGGTAAGATGAACA+AGG 0.613106 2_4:+49191955 None:intergenic
GATCAACTATCTGACGGCCA+GGG 0.614613 2_4:+49191632 None:intergenic
AATCGCGGTGTTCTAGCAGT+CGG 0.621032 2_4:+49191739 None:intergenic
CACTTGGTAACTCAAAGACC+AGG 0.624367 2_4:-49191300 Msa0332280:CDS
GATGATCTTGAACTCACACT+TGG 0.629133 2_4:-49191316 Msa0332280:CDS
AAGGGGATGATGATGGACTT+GGG 0.629431 2_4:+49191914 None:intergenic
CTGAAGATGAAGCTGTGGAG+AGG 0.631008 2_4:+49191839 None:intergenic
GCAGTCGGAGAGCTCAGGGG+AGG 0.643740 2_4:+49191754 None:intergenic
TGATCCTGAAGATGAAGCTG+TGG 0.648099 2_4:+49191834 None:intergenic
GGAGAAGTTGGACTAGCATG+TGG 0.648324 2_4:+49191583 None:intergenic
TAATGTTGTCCAGTCCACCC+GGG 0.661081 2_4:+49191685 None:intergenic
ACATGCCCCTGACTGTCCAG+GGG 0.666992 2_4:+49191483 None:intergenic
CTAGCAGTCGGAGAGCTCAG+GGG 0.684940 2_4:+49191751 None:intergenic
ATTCAGGATCAACTATCTGA+CGG 0.695991 2_4:+49191626 None:intergenic
TTGGATGGTAAGATGAACAA+GGG 0.701552 2_4:+49191956 None:intergenic
ACTTCGATGATCAATCTGCA+CGG 0.712290 2_4:-49191708 Msa0332280:CDS
TCGAAGTCAGCATTCAATCG+CGG 0.720698 2_4:+49191724 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAATGCAAATTCATCTGAAA+TGG + chr2_4:49191859-49191878 None:intergenic 25.0%
! ATTTCAGATGAATTTGCATT+TGG - chr2_4:49191858-49191877 Msa0332280:CDS 25.0%
!! TGATAATGATAATAAGTACC+TGG + chr2_4:49191982-49192001 None:intergenic 25.0%
AAATAAGGATGAGGAAGTTT+CGG + chr2_4:49191456-49191475 None:intergenic 30.0%
TTGGATGGTAAGATGAACAA+GGG + chr2_4:49191308-49191327 None:intergenic 35.0%
ATGGTAATTCTCTCATTCCA+TGG - chr2_4:49191391-49191410 Msa0332280:CDS 35.0%
CCACTATGAAAATAAGGATG+AGG + chr2_4:49191465-49191484 None:intergenic 35.0%
!!! CCTCATCCTTATTTTCATAG+TGG - chr2_4:49191462-49191481 Msa0332280:CDS 35.0%
ATGGAGCCACTATGAAAATA+AGG + chr2_4:49191471-49191490 None:intergenic 35.0%
ATTCAGGATCAACTATCTGA+CGG + chr2_4:49191638-49191657 None:intergenic 35.0%
CGAGATACAAGATTGAATGT+CGG + chr2_4:49191702-49191721 None:intergenic 35.0%
!! TGAAAAAGCATCTTCCTTGA+AGG + chr2_4:49191736-49191755 None:intergenic 35.0%
!!! TTCAAGGAAGATGCTTTTTC+AGG - chr2_4:49191735-49191754 Msa0332280:CDS 35.0%
CATTTGGAAGCAACACATTT+GGG - chr2_4:49191874-49191893 Msa0332280:CDS 35.0%
CATCTGCTACAAATTCTTCA+TGG + chr2_4:49191929-49191948 None:intergenic 35.0%
CTTGGATGGTAAGATGAACA+AGG + chr2_4:49191309-49191328 None:intergenic 40.0%
ACTTCGATGATCAATCTGCA+CGG - chr2_4:49191553-49191572 Msa0332280:CDS 40.0%
GTCAGATAGTTGATCCTGAA+TGG - chr2_4:49191637-49191656 Msa0332280:CDS 40.0%
!! TTGATCCTGAATGGTTTGCT+GGG - chr2_4:49191646-49191665 Msa0332280:CDS 40.0%
AGATTGAATGTCGGAGAAGT+TGG + chr2_4:49191693-49191712 None:intergenic 40.0%
CATCTTCCTTGAAGGCAAAT+GGG + chr2_4:49191728-49191747 None:intergenic 40.0%
! TCTGAAATGGCTTCAGACAT+TGG + chr2_4:49191846-49191865 None:intergenic 40.0%
GCATTTGGAAGCAACACATT+TGG - chr2_4:49191873-49191892 Msa0332280:CDS 40.0%
! ATTTGGGATTGTGAAGCCTT+GGG - chr2_4:49191890-49191909 Msa0332280:CDS 40.0%
GATGATCTTGAACTCACACT+TGG - chr2_4:49191945-49191964 Msa0332280:CDS 40.0%
!! AAGGGGATGATGATGGACTT+GGG + chr2_4:49191350-49191369 None:intergenic 45.0%
!! TAAGGGGATGATGATGGACT+TGG + chr2_4:49191351-49191370 None:intergenic 45.0%
GTTACGATTTGCAGCGTAAG+GGG + chr2_4:49191367-49191386 None:intergenic 45.0%
CGTTACGATTTGCAGCGTAA+GGG + chr2_4:49191368-49191387 None:intergenic 45.0%
TGATCCTGAAGATGAAGCTG+TGG + chr2_4:49191430-49191449 None:intergenic 45.0%
TCGAAGTCAGCATTCAATCG+CGG + chr2_4:49191540-49191559 None:intergenic 45.0%
! GTTGATCCTGAATGGTTTGC+TGG - chr2_4:49191645-49191664 Msa0332280:CDS 45.0%
TTGATCCCAGCAAACCATTC+AGG + chr2_4:49191654-49191673 None:intergenic 45.0%
GCATCTTCCTTGAAGGCAAA+TGG + chr2_4:49191729-49191748 None:intergenic 45.0%
!!! GAAGATGCTTTTTCAGGCAG+CGG - chr2_4:49191741-49191760 Msa0332280:CDS 45.0%
! CATTTGGGATTGTGAAGCCT+TGG - chr2_4:49191889-49191908 Msa0332280:CDS 45.0%
! CACTTGGTAACTCAAAGACC+AGG - chr2_4:49191961-49191980 Msa0332280:CDS 45.0%
TCATCTTACCATCCAAGCCC+CGG - chr2_4:49191312-49191331 Msa0332280:CDS 50.0%
!! GGATGATGATGGACTTGGGA+AGG + chr2_4:49191346-49191365 None:intergenic 50.0%
GCGTTACGATTTGCAGCGTA+AGG + chr2_4:49191369-49191388 None:intergenic 50.0%
GCTGCAAATCGTAACGCTGA+TGG - chr2_4:49191372-49191391 Msa0332280:CDS 50.0%
!! GTGGAGAGGTTTTTGAGCCA+TGG + chr2_4:49191411-49191430 None:intergenic 50.0%
CTGAAGATGAAGCTGTGGAG+AGG + chr2_4:49191425-49191444 None:intergenic 50.0%
CTCTCCACAGCTTCATCTTC+AGG - chr2_4:49191423-49191442 Msa0332280:CDS 50.0%
AATCGCGGTGTTCTAGCAGT+CGG + chr2_4:49191525-49191544 None:intergenic 50.0%
TAATGTTGTCCAGTCCACCC+GGG + chr2_4:49191579-49191598 None:intergenic 50.0%
GTAATGTTGTCCAGTCCACC+CGG + chr2_4:49191580-49191599 None:intergenic 50.0%
TACTCCTTCCTGCCTTCTTC+CGG - chr2_4:49191597-49191616 Msa0332280:CDS 50.0%
GATCAACTATCTGACGGCCA+GGG + chr2_4:49191632-49191651 None:intergenic 50.0%
!! GGAGAAGTTGGACTAGCATG+TGG + chr2_4:49191681-49191700 None:intergenic 50.0%
TCGAAGCCCATTTGCCTTCA+AGG - chr2_4:49191719-49191738 Msa0332280:CDS 50.0%
TGTTCTCCAGCTATTGCAGC+AGG - chr2_4:49191798-49191817 Msa0332280:CDS 50.0%
TCAAAGCCTGCTGCAATAGC+TGG + chr2_4:49191807-49191826 None:intergenic 50.0%
TGAAGCCTTGGGAAGGAGAA+AGG - chr2_4:49191901-49191920 Msa0332280:CDS 50.0%
TGGATCCTTTCTCCTTCCCA+AGG + chr2_4:49191909-49191928 None:intergenic 50.0%
ACTTGGGAAGGAAGACGAGC+CGG + chr2_4:49191334-49191353 None:intergenic 55.0%
GCAGCGTAAGGGGATGATGA+TGG + chr2_4:49191357-49191376 None:intergenic 55.0%
!! AGGTGTGACAGGAGCACTGA+TGG + chr2_4:49191490-49191509 None:intergenic 55.0%
TCTAGCAGTCGGAGAGCTCA+GGG + chr2_4:49191514-49191533 None:intergenic 55.0%
TTCTAGCAGTCGGAGAGCTC+AGG + chr2_4:49191515-49191534 None:intergenic 55.0%
GATGATCAATCTGCACGGCC+CGG - chr2_4:49191558-49191577 Msa0332280:CDS 55.0%
ATGATCAATCTGCACGGCCC+GGG - chr2_4:49191559-49191578 Msa0332280:CDS 55.0%
GGATCAACTATCTGACGGCC+AGG + chr2_4:49191633-49191652 None:intergenic 55.0%
AACATGCCCCTGACTGTCCA+GGG + chr2_4:49191782-49191801 None:intergenic 55.0%
GGGATTGTGAAGCCTTGGGA+AGG - chr2_4:49191894-49191913 Msa0332280:CDS 55.0%
TTGGGAAGGAAGACGAGCCG+GGG + chr2_4:49191332-49191351 None:intergenic 60.0%
CTTGGGAAGGAAGACGAGCC+GGG + chr2_4:49191333-49191352 None:intergenic 60.0%
CTAGCAGTCGGAGAGCTCAG+GGG + chr2_4:49191513-49191532 None:intergenic 60.0%
AGGACCGGAAGAAGGCAGGA+AGG + chr2_4:49191604-49191623 None:intergenic 60.0%
TTGGAGGACCGGAAGAAGGC+AGG + chr2_4:49191608-49191627 None:intergenic 60.0%
ACTATCTGACGGCCAGGGCT+TGG + chr2_4:49191627-49191646 None:intergenic 60.0%
ATGTGGACCCCTGGACAGTC+AGG - chr2_4:49191771-49191790 Msa0332280:CDS 60.0%
TGTGGACCCCTGGACAGTCA+GGG - chr2_4:49191772-49191791 Msa0332280:CDS 60.0%
ACATGCCCCTGACTGTCCAG+GGG + chr2_4:49191781-49191800 None:intergenic 60.0%
GAACATGCCCCTGACTGTCC+AGG + chr2_4:49191783-49191802 None:intergenic 60.0%
AAGACGAGCCGGGGCTTGGA+TGG + chr2_4:49191323-49191342 None:intergenic 65.0%
AAGGAAGACGAGCCGGGGCT+TGG + chr2_4:49191327-49191346 None:intergenic 65.0%
! GAGCTCAGGGGAGGTGTGAC+AGG + chr2_4:49191501-49191520 None:intergenic 65.0%
ATCAATCTGCACGGCCCGGG+TGG - chr2_4:49191562-49191581 Msa0332280:CDS 65.0%
GGGCTTGGAGGACCGGAAGA+AGG + chr2_4:49191612-49191631 None:intergenic 65.0%
TCTTCCGGTCCTCCAAGCCC+TGG - chr2_4:49191612-49191631 Msa0332280:CDS 65.0%
ATCTGACGGCCAGGGCTTGG+AGG + chr2_4:49191624-49191643 None:intergenic 65.0%
GTGGACCCCTGGACAGTCAG+GGG - chr2_4:49191773-49191792 Msa0332280:CDS 65.0%
GCAGTCGGAGAGCTCAGGGG+AGG + chr2_4:49191510-49191529 None:intergenic 70.0%
ACGGCCAGGGCTTGGAGGAC+CGG + chr2_4:49191619-49191638 None:intergenic 70.0%
CAGGCAGCGGTTCGCGCATG+TGG - chr2_4:49191754-49191773 Msa0332280:CDS 70.0%
GGTTCGCGCATGTGGACCCC+TGG - chr2_4:49191762-49191781 Msa0332280:CDS 70.0%
TCTGCACGGCCCGGGTGGAC+TGG - chr2_4:49191567-49191586 Msa0332280:CDS 75.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 49191273 49192010 49191273 ID=Msa0332280;Name=Msa0332280
chr2_4 mRNA 49191273 49192010 49191273 ID=Msa0332280-mRNA-1;Parent=Msa0332280;Name=Msa0332280-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|245
chr2_4 exon 49191273 49192010 49191273 ID=Msa0332280-mRNA-1:exon:14761;Parent=Msa0332280-mRNA-1
chr2_4 CDS 49191273 49192010 49191273 ID=Msa0332280-mRNA-1:cds;Parent=Msa0332280-mRNA-1
Gene Sequence

>Msa0332280

ATGGATATGGTTGGTGGATCATCAGCTGCAAGCCCTTGTTCATCTTACCATCCAAGCCCCGGCTCGTCTTCCTTCCCAAGTCCATCATCATCCCCTTACGCTGCAAATCGTAACGCTGATGGTAATTCTCTCATTCCATGGCTCAAAAACCTCTCCACAGCTTCATCTTCAGGATCATCTCCGAAACTTCCTCATCCTTATTTTCATAGTGGCTCCATCAGTGCTCCTGTCACACCTCCCCTGAGCTCTCCGACTGCTAGAACACCGCGATTGAATGCTGACTTCGATGATCAATCTGCACGGCCCGGGTGGACTGGACAACATTACTCCTTCCTGCCTTCTTCCGGTCCTCCAAGCCCTGGCCGTCAGATAGTTGATCCTGAATGGTTTGCTGGGATCAAACTTCCACATGCTAGTCCAACTTCTCCGACATTCAATCTTGTATCTCGAAGCCCATTTGCCTTCAAGGAAGATGCTTTTTCAGGCAGCGGTTCGCGCATGTGGACCCCTGGACAGTCAGGGGCATGTTCTCCAGCTATTGCAGCAGGCTTTGATCAAACTGCTGACATTCCAATGTCTGAAGCCATTTCAGATGAATTTGCATTTGGAAGCAACACATTTGGGATTGTGAAGCCTTGGGAAGGAGAAAGGATCCATGAAGAATTTGTAGCAGATGATCTTGAACTCACACTTGGTAACTCAAAGACCAGGTACTTATTATCATTATCATCATTGTAG
Protein sequence

>Msa0332280

MDMVGGSSAASPCSSYHPSPGSSSFPSPSSSPYAANRNADGNSLIPWLKNLSTASSSGSSPKLPHPYFHSGSISAPVTPPLSSPTARTPRLNADFDDQSARPGWTGQHYSFLPSSGPPSPGRQIVDPEWFAGIKLPHASPTSPTFNLVSRSPFAFKEDAFSGSGSRMWTPGQSGACSPAIAAGFDQTADIPMSEAISDEFAFGSNTFGIVKPWEGERIHEEFVADDLELTLGNSKTRYLLSLSSL*