AlfalfaGEDB | Msa0655710

M. sativa cultivar zhongmu-4 / Msa0655710

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0655710	XP_039684437.1	98.496	133	0	1	1	133	34	164	7.52e-90	268

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006840	Msa0655710	0.829630	4.621845e-55	-8.615850e-47
Msa0009780	Msa0655710	0.820549	6.468388e-53	-8.615850e-47
Msa0030070	Msa0655710	0.812188	4.807907e-51	-8.615850e-47
Msa0050530	Msa0655710	0.841067	5.947808e-58	-8.615850e-47
Msa0069350	Msa0655710	0.803182	3.938354e-49	-8.615850e-47
Msa0100600	Msa0655710	0.832126	1.128996e-55	-8.615850e-47
Msa0100890	Msa0655710	0.842631	2.298898e-58	-8.615850e-47
Msa0104570	Msa0655710	0.823006	1.746867e-53	-8.615850e-47
Msa0125590	Msa0655710	0.805625	1.219818e-49	-8.615850e-47
Msa0134830	Msa0655710	0.812902	3.355094e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0655710	MtrunA17_Chr8g0375551	98.496	133	0	1	1	133	34	164	6.92e-94	268
Msa0655710	MtrunA17_Chr5g0410051	36.842	76	47	1	14	89	50	124	7.58e-14	64.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0655710	AT1G31940.1	46.939	49	26	0	18	66	53	101	3.22e-12	60.5
Msa0655710	AT2G35585.1	41.818	55	31	1	14	67	48	102	4.84e-11	57.4

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Find 68 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGAATCCCTCCTTGCA+TGG	0.531998	4_3:-64577633	None:intergenic
AAAGGAGAGTGAGCATAAAA+AGG	0.300414	4_3:-64576086	None:intergenic
AACGACATATGATGAAAACT+TGG	0.485073	4_3:-64577721	None:intergenic
AACTTAAAATAGAGCAAGAC+CGG	0.519643	4_3:+64577676	Msa0655710:three_prime_UTR
AATTGGAGAATTTATTGATA+CGG	0.474812	4_3:+64576303	Msa0655710:five_prime_UTR
ACACTAATGACTGAAGATTC+AGG	0.437311	4_3:-64576630	None:intergenic
ACAGAAAGCATAAGGAAGAG+AGG	0.581626	4_3:+64577372	Msa0655710:CDS
ACCACCATCATCATCATCAG+AGG	0.627709	4_3:+64576526	Msa0655710:CDS
ACCCTCACTCATGGATCATC+AGG	0.442814	4_3:+64577474	Msa0655710:CDS
ACCGGACGATCTTCATCCGG+TGG	0.608873	4_3:-64576594	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAGGAGTAAGAGAGAAT+GGG	-	chr4_3:64576073-64576092	None:intergenic	30.0%
TAAAAAGGAGTAAGAGAGAA+TGG	-	chr4_3:64576074-64576093	None:intergenic	30.0%
TTTCTTGTCCATTGATCAAA+AGG	+	chr4_3:64576278-64576297	Msa0655710:five_prime_UTR	30.0%
TTCTTGTCCATTGATCAAAA+GGG	+	chr4_3:64576279-64576298	Msa0655710:five_prime_UTR	30.0%
GTTACCTACTTATCTTAAGA+TGG	+	chr4_3:64576676-64576695	Msa0655710:intron	30.0%
TTTGCCATCTTAAGATAAGT+AGG	-	chr4_3:64576683-64576702	None:intergenic	30.0%
ATTATATGAACACGACTACA+AGG	-	chr4_3:64576806-64576825	None:intergenic	30.0%
ACCAATTTAGAAATTTCAGG+TGG	+	chr4_3:64576854-64576873	Msa0655710:intron	30.0%
ACCACCTGAAATTTCTAAAT+TGG	-	chr4_3:64576858-64576877	None:intergenic	30.0%
AATGTTTGGCAACAATTTAC+CGG	+	chr4_3:64577165-64577184	Msa0655710:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	64576040	64577951	64576040	ID=Msa0655710;Name=Msa0655710
chr4_3	mRNA	64576040	64577951	64576040	ID=Msa0655710-mRNA-1;Parent=Msa0655710;Name=Msa0655710-mRNA-1;_AED=0.16;_eAED=0.16;_QI=351\|1\|1\|1\|0.5\|0.66\|3\|346\|133
chr4_3	exon	64576040	64576324	64576040	ID=Msa0655710-mRNA-1:exon:18268;Parent=Msa0655710-mRNA-1
chr4_3	exon	64576444	64576650	64576444	ID=Msa0655710-mRNA-1:exon:18269;Parent=Msa0655710-mRNA-1
chr4_3	exon	64577345	64577951	64577345	ID=Msa0655710-mRNA-1:exon:18270;Parent=Msa0655710-mRNA-1
chr4_3	five_prime_UTR	64576040	64576324	64576040	ID=Msa0655710-mRNA-1:five_prime_utr;Parent=Msa0655710-mRNA-1
chr4_3	five_prime_UTR	64576444	64576509	64576444	ID=Msa0655710-mRNA-1:five_prime_utr;Parent=Msa0655710-mRNA-1
chr4_3	CDS	64576510	64576650	64576510	ID=Msa0655710-mRNA-1:cds;Parent=Msa0655710-mRNA-1
chr4_3	CDS	64577345	64577605	64577345	ID=Msa0655710-mRNA-1:cds;Parent=Msa0655710-mRNA-1
chr4_3	three_prime_UTR	64577606	64577951	64577606	ID=Msa0655710-mRNA-1:three_prime_utr;Parent=Msa0655710-mRNA-1

Gene Sequence

>Msa0655710

ATGGAAGTGTATCATCACCACCATCATCATCATCAGAGGGTTGATAACAACACAGATTTTTTCCCAGTTGAACATCCTATGGAGCCACCGGATGAAGATCGTCCGGTGAAATGTCCAATGCCTGAATCTTCAGTCATTAGTGATGAAAGGATGCATGAAAAGAGGAACACAGAAAGCATAAGGAAGAGAGGTGAGATGTCTGGGTCTGGACAAAGAACAGCTGGCATGGACACAGATGCCCCAGCTCGAGGAGTGCGAAAGCGGCATCACACCCTCACTCATGGATCATCAGGAGACATTGTGATGACTCCATTGATGAGAATGCCACATCTCCCTCCTCTACAATCCCAGAATATCACTATTTTCCAAGTGCTTCAGCAGTTGGACAAGTTTGAGTCTTAA

Protein sequence

>Msa0655710

MEVYHHHHHHHQRVDNNTDFFPVEHPMEPPDEDRPVKCPMPESSVISDERMHEKRNTESIRKRGEMSGSGQRTAGMDTDAPARGVRKRHHTLTHGSSGDIVMTPLMRMPHLPPLQSQNITIFQVLQQLDKFES*