AlfalfaGEDB | Msa0413200

M. sativa cultivar zhongmu-4 / Msa0413200

KEGG: K01103
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005975 GO:0005996 GO:0006000 GO:0006002 GO:0006003 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0019318 GO:0019637 GO:0043609 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0065007 GO:0071704 GO:0071944 GO:1901135
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0413200	GAU27680.1	88.372	86	10	0	1	86	1	86	1.38e-43	160

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0413200	sp\|Q9MB58\|F26_ARATH	71.930	57	16	0	30	86	22	78	6.99e-22	91.3

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0413200	A0A2Z6MVG1	88.372	86	10	0	1	86	1	86	6.58e-44	160

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0030310	Msa0413200	0.809669	1.687964e-50	-8.615850e-47
Msa0050730	Msa0413200	0.801130	1.041137e-48	-8.615850e-47
Msa0113160	Msa0413200	0.814244	1.700430e-51	-8.615850e-47
Msa0227510	Msa0413200	0.809081	2.256674e-50	-8.615850e-47
Msa0413200	Msa0469910	-0.813464	2.526619e-51	-8.615850e-47
Msa0413200	Msa0524200	-0.828830	7.228818e-55	-8.615850e-47
Msa0413200	Msa0614210	0.803542	3.317293e-49	-8.615850e-47
Msa0413200	Msa0638690	0.803817	2.908619e-49	-8.615850e-47
Msa0413200	Msa0681890	0.800892	1.164231e-48	-8.615850e-47
Msa0413200	Msa0686610	0.807857	4.119163e-50	-8.615850e-47
Msa0413200	Msa0697400	0.808268	3.367201e-50	-8.615850e-47
Msa0413200	Msa0768810	0.804549	2.048022e-49	-8.615850e-47
Msa0413200	Msa0836620	0.804282	2.327641e-49	-8.615850e-47
Msa0413200	Msa0852730	0.800936	1.140563e-48	-8.615850e-47
Msa0413200	Msa0958020	0.825084	5.684669e-54	-8.615850e-47
Msa0413200	Msa0959010	0.819660	1.033712e-52	-8.615850e-47
Msa0413200	Msa0993530	0.835981	1.222399e-56	-8.615850e-47
Msa0413200	Msa1015060	0.814608	1.413052e-51	-8.615850e-47
Msa0413200	Msa1030280	0.800437	1.442030e-48	-8.615850e-47
Msa0413200	Msa1092820	0.835512	1.607432e-56	-8.615850e-47
Msa0413200	Msa1221900	0.858741	6.688310e-63	-8.615850e-47
Msa0413200	Msa1237030	0.801062	1.074990e-48	-8.615850e-47
Msa0413200	Msa1265070	0.838622	2.578545e-57	-8.615850e-47
Msa0413200	Msa1316170	0.801161	1.026033e-48	-8.615850e-47
Msa0413200	Msa1327760	0.800092	1.695098e-48	-8.615850e-47
Msa0413200	Msa1337730	0.855305	6.895699e-62	-8.615850e-47
Msa0413200	Msa1367390	-0.823704	1.200435e-53	-8.615850e-47
Msa0413200	Msa1367940	0.801737	7.819473e-49	-8.615850e-47
Msa0413200	Msa1370650	0.806696	7.255072e-50	-8.615850e-47
Msa0413200	Msa1373510	0.810099	1.364237e-50	-8.615850e-47
Msa0413200	Msa1404720	0.803907	2.785738e-49	-8.615850e-47
Msa0413200	Msa1454750	0.810255	1.262092e-50	-8.615850e-47
Msa0328210	Msa0413200	0.811801	5.837274e-51	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0413200	MtrunA17_Chr3g0105421	90.698	86	8	0	1	86	1	86	8.17e-45	152
Msa0413200	MtrunA17_Chr5g0430761	50.562	89	35	3	1	86	1	83	5.44e-17	74.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0413200	AT1G07110.1	71.930	57	16	0	30	86	22	78	7.10e-23	91.3

Find 26 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATCTTGTTCCTCATGTTTA+TGG	0.069894	3_2:+57481504	Msa0413200:CDS
CTGTTCCTCTTGTTGGTTCT+TGG	0.098069	3_2:+57481529	Msa0413200:CDS
TTCATCATCTGTTCTGTCTT+TGG	0.170290	3_2:-57481418	None:intergenic
TTTATTGTCGATGGAAATTA+TGG	0.234310	3_2:-57481209	None:intergenic
TGTTCCTCTTGTTGGTTCTT+GGG	0.302993	3_2:+57481530	Msa0413200:CDS
TATGGTTCTGTTCCTCTTGT+TGG	0.322051	3_2:+57481522	Msa0413200:CDS
TTTGTTGGTGTGAGTATGTT+TGG	0.342073	3_2:-57481234	None:intergenic
TAGATATAAGTATAGATATA+TGG	0.353307	3_2:-57481279	None:intergenic
AGTGATGAGAATTACAGCTT+TGG	0.461412	3_2:-57481559	None:intergenic
AAGAAAGCATTACCGTGATT+AGG	0.484929	3_2:-57481704	None:intergenic
AAAGACAAGAACATGGGAAC+TGG	0.492342	3_2:+57481360	Msa0413200:exon
AAGTATAGATATATGGATAG+TGG	0.500881	3_2:-57481272	None:intergenic
ACTATATGTTTCTATCAAGA+TGG	0.516635	3_2:+57481461	Msa0413200:CDS
CAAGCACAAAGACAAGAACA+TGG	0.518285	3_2:+57481353	Msa0413200:exon
AGATATATGGATAGTGGATG+AGG	0.519744	3_2:-57481266	None:intergenic
CATGGGAACTGGTTCATCAA+AGG	0.563402	3_2:+57481371	Msa0413200:exon
TTTGTTGTTCCACCTAATCA+CGG	0.591139	3_2:+57481692	Msa0413200:CDS
AAAGCATTACCGTGATTAGG+TGG	0.594330	3_2:-57481701	None:intergenic
AAGAGAATCAGTTTCAATGT+GGG	0.617723	3_2:+57481661	Msa0413200:CDS
GAATCCCAAGAACCAACAAG+AGG	0.622320	3_2:-57481534	None:intergenic
AAAGAGAATCAGTTTCAATG+TGG	0.628033	3_2:+57481660	Msa0413200:CDS
GATGATGAAGATGATCAAGC+TGG	0.659255	3_2:+57481432	Msa0413200:CDS
AAGCACAAAGACAAGAACAT+GGG	0.665062	3_2:+57481354	Msa0413200:exon
GGAACAGAACCATAAACATG+AGG	0.668847	3_2:-57481513	None:intergenic
AATTATGGCAAATCATGCTG+CGG	0.670995	3_2:-57481194	None:intergenic
GATGAAGATGATCAAGCTGG+TGG	0.671413	3_2:+57481435	Msa0413200:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTTTTTTTTTCATTTC+TGG	+	chr3_2:57481587-57481606	Msa0413200:intron	10.0%
!!!	TTTTTTTTTTTTTCATTTCT+GGG	+	chr3_2:57481588-57481607	Msa0413200:intron	10.0%
!!	TAGATATAAGTATAGATATA+TGG	-	chr3_2:57481282-57481301	None:intergenic	15.0%
!!	AATTAGCAAAAAAAACAAAA+AGG	-	chr3_2:57481614-57481633	None:intergenic	15.0%
!!	NNNNNNNTAGCTATTATGTT+AGG	+	chr3_2:57481827-57481846	Msa0413200:intron	15.0%
!!!	TTGTTTTTTTTAGCTTTCTA+TGG	+	chr3_2:57481637-57481656	Msa0413200:intron	20.0%
!	TTTATTGTCGATGGAAATTA+TGG	-	chr3_2:57481212-57481231	None:intergenic	25.0%
!	AAGTATAGATATATGGATAG+TGG	-	chr3_2:57481275-57481294	None:intergenic	25.0%
!	GAAAAAAATGGTAATTCAGA+AGG	+	chr3_2:57481393-57481412	Msa0413200:CDS	25.0%
!!	ACTATATGTTTCTATCAAGA+TGG	+	chr3_2:57481461-57481480	Msa0413200:CDS	25.0%
!	TGTTTGGATTTTATTGTCGA+TGG	-	chr3_2:57481221-57481240	None:intergenic	30.0%
	GGTTCATCAAAGGAAAAAAA+TGG	+	chr3_2:57481381-57481400	Msa0413200:CDS	30.0%
	AAAGAGAATCAGTTTCAATG+TGG	+	chr3_2:57481660-57481679	Msa0413200:CDS	30.0%
	AAGAGAATCAGTTTCAATGT+GGG	+	chr3_2:57481661-57481680	Msa0413200:CDS	30.0%
	AATTATGGCAAATCATGCTG+CGG	-	chr3_2:57481197-57481216	None:intergenic	35.0%
	TTTGTTGGTGTGAGTATGTT+TGG	-	chr3_2:57481237-57481256	None:intergenic	35.0%
!!	ATGAGGATGTGTGTTTTTGT+TGG	-	chr3_2:57481252-57481271	None:intergenic	35.0%
!!	AGATATATGGATAGTGGATG+AGG	-	chr3_2:57481269-57481288	None:intergenic	35.0%
	AAGCACAAAGACAAGAACAT+GGG	+	chr3_2:57481354-57481373	Msa0413200:exon	35.0%
	TTCATCATCTGTTCTGTCTT+TGG	-	chr3_2:57481421-57481440	None:intergenic	35.0%
	CATCTTGTTCCTCATGTTTA+TGG	+	chr3_2:57481504-57481523	Msa0413200:CDS	35.0%
	AGTGATGAGAATTACAGCTT+TGG	-	chr3_2:57481562-57481581	None:intergenic	35.0%
!!	TTTGTTGTTCCACCTAATCA+CGG	+	chr3_2:57481692-57481711	Msa0413200:CDS	35.0%
!!	AAGAAAGCATTACCGTGATT+AGG	-	chr3_2:57481707-57481726	None:intergenic	35.0%
	CAAGCACAAAGACAAGAACA+TGG	+	chr3_2:57481353-57481372	Msa0413200:exon	40.0%
	AAAGACAAGAACATGGGAAC+TGG	+	chr3_2:57481360-57481379	Msa0413200:exon	40.0%
	GATGATGAAGATGATCAAGC+TGG	+	chr3_2:57481432-57481451	Msa0413200:CDS	40.0%
	GGAACAGAACCATAAACATG+AGG	-	chr3_2:57481516-57481535	None:intergenic	40.0%
!	TATGGTTCTGTTCCTCTTGT+TGG	+	chr3_2:57481522-57481541	Msa0413200:CDS	40.0%
!!	TGTTCCTCTTGTTGGTTCTT+GGG	+	chr3_2:57481530-57481549	Msa0413200:CDS	40.0%
!	AAAGCATTACCGTGATTAGG+TGG	-	chr3_2:57481704-57481723	None:intergenic	40.0%
!	CATGGGAACTGGTTCATCAA+AGG	+	chr3_2:57481371-57481390	Msa0413200:exon	45.0%
	GATGAAGATGATCAAGCTGG+TGG	+	chr3_2:57481435-57481454	Msa0413200:CDS	45.0%
!!	CTGTTCCTCTTGTTGGTTCT+TGG	+	chr3_2:57481529-57481548	Msa0413200:CDS	45.0%
	GAATCCCAAGAACCAACAAG+AGG	-	chr3_2:57481537-57481556	None:intergenic	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	57481188	57481868	57481188	ID=Msa0413200;Name=Msa0413200
chr3_2	mRNA	57481188	57481868	57481188	ID=Msa0413200-mRNA-1;Parent=Msa0413200;Name=Msa0413200-mRNA-1;_AED=0.02;_eAED=0.02;_QI=184\|0.5\|0.66\|0.66\|0.5\|0.66\|3\|0\|92
chr3_2	exon	57481188	57481566	57481188	ID=Msa0413200-mRNA-1:exon:11724;Parent=Msa0413200-mRNA-1
chr3_2	exon	57481650	57481713	57481650	ID=Msa0413200-mRNA-1:exon:11725;Parent=Msa0413200-mRNA-1
chr3_2	exon	57481849	57481868	57481849	ID=Msa0413200-mRNA-1:exon:11726;Parent=Msa0413200-mRNA-1
chr3_2	five_prime_UTR	57481188	57481371	57481188	ID=Msa0413200-mRNA-1:five_prime_utr;Parent=Msa0413200-mRNA-1
chr3_2	CDS	57481372	57481566	57481372	ID=Msa0413200-mRNA-1:cds;Parent=Msa0413200-mRNA-1
chr3_2	CDS	57481650	57481713	57481650	ID=Msa0413200-mRNA-1:cds;Parent=Msa0413200-mRNA-1
chr3_2	CDS	57481849	57481868	57481849	ID=Msa0413200-mRNA-1:cds;Parent=Msa0413200-mRNA-1

Gene Sequence	>Msa0413200 ATGGGAACTGGTTCATCAAAGGAAAAAAATGGTAATTCAGAAGGAACCAAAGACAGAACAGATGATGAAGATGATCAAGCTGGTGGACAACTATATGTTTCTATCAAGATGGAGAATCATAAACTCACTACTCATCTTGTTCCTCATGTTTATGGTTCTGTTCCTCTTGTTGGTTCTTGGGATTCTTCCAAAGCTCTTTCTATGGAAAGAGAATCAGTTTCAATGTGGGAGCTTAGTTTTGTTGTTCCACCTAATCACGGAAAAGAAAATCTTAGTTAG
Protein sequence	>Msa0413200 MGTGSSKEKNGNSEGTKDRTDDEDDQAGGQLYVSIKMENHKLTTHLVPHVYGSVPLVGSWDSSKALSMERESVSMWELSFVVPPNHGKENLS*