AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0486170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0486170 MtrunA17_Chr1g0211791 99.123 114 1 0 1 114 1 114 1.88e-78 226
Msa0486170 MtrunA17_Chr4g0014331 33.663 101 67 0 11 111 96 196 7.89e-17 72.4
Msa0486170 MtrunA17_Chr3g0081601 29.126 103 73 0 11 113 80 182 2.79e-13 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0486170 AT5G27820.1 79.825 114 23 0 1 114 1 114 4.00e-65 192
Msa0486170 AT3G20230.1 35.644 101 65 0 11 111 83 183 7.22e-19 77.8
Msa0486170 AT3G45020.3 36.937 111 58 4 16 114 4 114 3.40e-13 61.6
Msa0486170 AT3G45020.2 36.937 111 58 4 16 114 4 114 3.40e-13 61.6
Msa0486170 AT3G45020.1 36.937 111 58 4 16 114 4 114 3.40e-13 61.6
Msa0486170 AT3G45020.4 36.937 111 58 4 16 114 4 114 3.40e-13 61.6
Msa0486170 AT1G08845.1 27.184 103 75 0 11 113 82 184 2.06e-12 60.8
Msa0486170 AT1G08845.3 27.184 103 75 0 11 113 82 184 2.06e-12 60.8
Msa0486170 AT1G08845.2 27.184 103 75 0 11 113 127 229 2.80e-12 61.2

Find 67 sgRNAs with CRISPR-Local

Find 292 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATTAGATGATTGTTGTTT+TGG 0.263030 3_4:-3946128 Msa0486170:three_prime_UTR
ATGAGCAGCTCAGGTTTAAT+TGG 0.282596 3_4:+3946233 None:intergenic
TTTCAGATATTGTGTAAGTC+TGG 0.313396 3_4:+3946661 None:intergenic
CCGACTAGTGTATGGTGTTT+TGG 0.318023 3_4:-3945999 Msa0486170:three_prime_UTR
CAACATCCCGAGTAGTTTCC+AGG 0.336475 3_4:+3946522 None:intergenic
CCTTTCTCGCCGTCACAAAC+AGG 0.362328 3_4:-3947987 Msa0486170:intron
CTGGGAGGCCTGACTGGAGG+TGG 0.388017 3_4:+3946680 None:intergenic
CCTGTTTGTGACGGCGAGAA+AGG 0.396026 3_4:+3947987 None:intergenic
TGGTTTGCAGAGTTGCAAAT+TGG 0.397335 3_4:-3946049 Msa0486170:three_prime_UTR
AAGCGGTTATTGATTCTCTC+AGG 0.400454 3_4:-3946391 Msa0486170:CDS
GTAAGTCTGGGAGGCCTGAC+TGG 0.402204 3_4:+3946674 None:intergenic
ATGTTTGATGAGCAACTTGA+TGG 0.413605 3_4:+3946186 None:intergenic
AAATGCATCTTAAGAACATA+AGG 0.422008 3_4:+3946638 None:intergenic
TTTCTGCAAAACAGATTTCT+CGG 0.428368 3_4:-3948095 Msa0486170:five_prime_UTR
GAAAGCCTTGAGATCAAGCC+TGG 0.444737 3_4:-3946540 Msa0486170:CDS
CTCCTGTGAGCTTGCAGAAG+AGG 0.447057 3_4:+3946562 None:intergenic
ATTGATTCTCTCAGGGAAGC+TGG 0.452667 3_4:-3946383 Msa0486170:CDS
AAGAGAGAACAGAGGTATCA+TGG 0.461247 3_4:-3946422 Msa0486170:CDS
GATGCTAAAACATTGTTTCG+TGG 0.462569 3_4:-3946082 Msa0486170:three_prime_UTR
ACCTTTGATCACTCGTTCGC+AGG 0.465646 3_4:+3946286 None:intergenic
ATGGGAAGGGAGTGGCTGAC+CGG 0.468800 3_4:+3948016 None:intergenic
GTTGCTGCAGCTGCAAAGAT+TGG 0.469361 3_4:-3946503 Msa0486170:CDS
GAGAAAGGAACGGGATGGGA+AGG 0.469987 3_4:+3948002 None:intergenic
AGTTTCCAGGCTTGATCTCA+AGG 0.483054 3_4:+3946535 None:intergenic
TGCAGAGTTGCAAATTGGTA+AGG 0.490455 3_4:-3946044 Msa0486170:three_prime_UTR
TGGTTATCCCACCTCCAGTC+AGG 0.492573 3_4:-3946688 Msa0486170:CDS
TGTTTCGTGGCAAGCGTAAG+TGG 0.503318 3_4:-3946069 Msa0486170:three_prime_UTR
TGTGACGGCGAGAAAGGAAC+GGG 0.503324 3_4:+3947993 None:intergenic
TTCAGATATTGTGTAAGTCT+GGG 0.506970 3_4:+3946662 None:intergenic
TGCCTCTTCTGCAAGCTCAC+AGG 0.512391 3_4:-3946564 Msa0486170:CDS
GAAGAGGCAACGGTAGCAGT+TGG 0.531232 3_4:+3946578 None:intergenic
CGGAGTGGACGTGACGGCGG+CGG 0.531524 3_4:+3948036 None:intergenic
GACTGCCACCGACTAGTGTA+TGG 0.534873 3_4:-3946007 Msa0486170:three_prime_UTR
GATCAAGCCTGGAAACTACT+CGG 0.540330 3_4:-3946529 Msa0486170:CDS
CGGCGAGAAAGGAACGGGAT+GGG 0.542349 3_4:+3947998 None:intergenic
AGCGGAACGCCTTTCGCTCA+AGG 0.547714 3_4:-3946471 Msa0486170:CDS
TGCACGAGAGAAAGAGCAGT+AGG 0.549319 3_4:+3948063 None:intergenic
CAGGAATGTCCTTGAGCGAA+AGG 0.550217 3_4:+3946462 None:intergenic
TTGCTGCAGCTGCAAAGATT+GGG 0.550997 3_4:-3946502 Msa0486170:CDS
GGAACGGGATGGGAAGGGAG+TGG 0.552945 3_4:+3948008 None:intergenic
AGAAAGGAACGGGATGGGAA+GGG 0.556403 3_4:+3948003 None:intergenic
AGCGGTTATTGATTCTCTCA+GGG 0.559363 3_4:-3946390 Msa0486170:CDS
TTCAAGTGAACAGAAACAGC+AGG 0.564698 3_4:+3946443 None:intergenic
AAGGGAGTGGCTGACCGGAG+TGG 0.568568 3_4:+3948021 None:intergenic
CGCCGCCGTCACGTCCACTC+CGG 0.571619 3_4:-3948035 Msa0486170:five_prime_UTR
TGGGAGGCCTGACTGGAGGT+GGG 0.577993 3_4:+3946681 None:intergenic
TCACTCGTTCGCAGGAGGCA+AGG 0.580899 3_4:+3946294 None:intergenic
TTGTGACGGCGAGAAAGGAA+CGG 0.588187 3_4:+3947992 None:intergenic
AGAACAGAGGTATCATGGAA+AGG 0.588862 3_4:-3946417 Msa0486170:CDS
ATCAAGCCTGGAAACTACTC+GGG 0.593151 3_4:-3946528 Msa0486170:CDS
ACGGCGAGAAAGGAACGGGA+TGG 0.594078 3_4:+3947997 None:intergenic
TCCTGCGAACGAGTGATCAA+AGG 0.598619 3_4:-3946287 Msa0486170:three_prime_UTR
AGTCTGGGAGGCCTGACTGG+AGG 0.599060 3_4:+3946677 None:intergenic
CAACGGTAGCAGTTGGAGTG+TGG 0.604975 3_4:+3946585 None:intergenic
CCAAAACACCATACACTAGT+CGG 0.605999 3_4:+3945999 None:intergenic
ATGTTCGAACCTGTTTGTGA+CGG 0.615764 3_4:+3947978 None:intergenic
GCACGAGAGAAAGAGCAGTA+GGG 0.621635 3_4:+3948064 None:intergenic
TTTGATCACTCGTTCGCAGG+AGG 0.626527 3_4:+3946289 None:intergenic
GACCGGAGTGGACGTGACGG+CGG 0.630763 3_4:+3948033 None:intergenic
TGAGCTTGCAGAAGAGGCAA+CGG 0.638179 3_4:+3946568 None:intergenic
AGATATTGTGTAAGTCTGGG+AGG 0.649085 3_4:+3946665 None:intergenic
GTATCATGGAAAGGTAAAAG+CGG 0.664247 3_4:-3946408 Msa0486170:CDS
GCTGACCGGAGTGGACGTGA+CGG 0.677474 3_4:+3948030 None:intergenic
GCAGGAGGCAAGGTTCAACA+AGG 0.686635 3_4:+3946304 None:intergenic
AAACACCATACACTAGTCGG+TGG 0.700254 3_4:+3946002 None:intergenic
AAAGATTGGGAAGCTACTAG+CGG 0.716694 3_4:-3946489 Msa0486170:CDS
TTCACTTGAAGAGAGAACAG+AGG 0.737493 3_4:-3946430 Msa0486170:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTGAATTGATTTAAAAA+GGG + chr3_4:3946372-3946391 None:intergenic 10.0%
!!! TTAATTGAATTGATTTAAAA+AGG + chr3_4:3946373-3946392 None:intergenic 10.0%
!!! TAATTGAATTGATTTAAAAA+GGG + chr3_4:3946372-3946391 None:intergenic 10.0%
!!! TTAATTGAATTGATTTAAAA+AGG + chr3_4:3946373-3946392 None:intergenic 10.0%
!!! AATTGAATTGATTTAAAAAG+GGG + chr3_4:3946371-3946390 None:intergenic 15.0%
!! AATATACATTTAATAACACT+TGG + chr3_4:3946623-3946642 None:intergenic 15.0%
!!! AATTGAATTGATTTAAAAAG+GGG + chr3_4:3946371-3946390 None:intergenic 15.0%
!! AATATACATTTAATAACACT+TGG + chr3_4:3946623-3946642 None:intergenic 15.0%
!!! CAGTATATTTTTAACTGTTT+TGG - chr3_4:3946577-3946596 Msa0486170:CDS 20.0%
!! TCTAAACATTATTAGTTCAA+GGG + chr3_4:3946752-3946771 None:intergenic 20.0%
!! TTCTAAACATTATTAGTTCA+AGG + chr3_4:3946753-3946772 None:intergenic 20.0%
!!! ACTTCTTTATTCTTTTTAAG+GGG + chr3_4:3946932-3946951 None:intergenic 20.0%
!!! CACTTCTTTATTCTTTTTAA+GGG + chr3_4:3946933-3946952 None:intergenic 20.0%
!! ATAAAATAGTGATACCAAAA+TGG + chr3_4:3947169-3947188 None:intergenic 20.0%
!!! TCATTTAATGTTTTAACAAC+AGG + chr3_4:3947316-3947335 None:intergenic 20.0%
!! TTAAATGAACTTGTAATTTG+TGG - chr3_4:3947328-3947347 Msa0486170:intron 20.0%
!!! ATATTAGATGATTGTTGTTT+TGG - chr3_4:3948006-3948025 Msa0486170:five_prime_UTR 20.0%
!!! CAGTATATTTTTAACTGTTT+TGG - chr3_4:3946577-3946596 Msa0486170:CDS 20.0%
!! TCTAAACATTATTAGTTCAA+GGG + chr3_4:3946752-3946771 None:intergenic 20.0%
!! TTCTAAACATTATTAGTTCA+AGG + chr3_4:3946753-3946772 None:intergenic 20.0%
!!! ACTTCTTTATTCTTTTTAAG+GGG + chr3_4:3946932-3946951 None:intergenic 20.0%
!!! CACTTCTTTATTCTTTTTAA+GGG + chr3_4:3946933-3946952 None:intergenic 20.0%
!! ATAAAATAGTGATACCAAAA+TGG + chr3_4:3947169-3947188 None:intergenic 20.0%
!!! TCATTTAATGTTTTAACAAC+AGG + chr3_4:3947316-3947335 None:intergenic 20.0%
!! TTAAATGAACTTGTAATTTG+TGG - chr3_4:3947328-3947347 Msa0486170:intron 20.0%
!!! ATATTAGATGATTGTTGTTT+TGG - chr3_4:3948006-3948025 Msa0486170:five_prime_UTR 20.0%
!!! TTATTTTTATTGCTGTGACT+TGG - chr3_4:3946290-3946309 Msa0486170:three_prime_UTR 25.0%
! ATATCACAGTAAAATATGAG+AGG + chr3_4:3946431-3946450 None:intergenic 25.0%
! CAGTTAAAAATATACTGAAC+TGG + chr3_4:3946574-3946593 None:intergenic 25.0%
! ATGTTACGATATTCTGATAT+AGG - chr3_4:3946644-3946663 Msa0486170:CDS 25.0%
!!! ATGAGATTGACTTTCATTTT+TGG + chr3_4:3946789-3946808 None:intergenic 25.0%
! AGTCAATCTCATGAAAATTT+AGG - chr3_4:3946797-3946816 Msa0486170:intron 25.0%
! GTCAATCTCATGAAAATTTA+GGG - chr3_4:3946798-3946817 Msa0486170:intron 25.0%
! TTATTATCATTACGAGCATA+TGG - chr3_4:3946841-3946860 Msa0486170:intron 25.0%
!!! CAAAAGATAAGTTTTATGGT+TGG + chr3_4:3946869-3946888 None:intergenic 25.0%
!! GAAGCAAAAGATAAGTTTTA+TGG + chr3_4:3946873-3946892 None:intergenic 25.0%
!! CCACTTCTTTATTCTTTTTA+AGG + chr3_4:3946934-3946953 None:intergenic 25.0%
! CCTTAAAAAGAATAAAGAAG+TGG - chr3_4:3946931-3946950 Msa0486170:intron 25.0%
! ACTAATATGTGATCTTACAA+TGG - chr3_4:3947084-3947103 Msa0486170:intron 25.0%
! GTTATATTGCAAAACAGAAA+TGG - chr3_4:3947426-3947445 Msa0486170:intron 25.0%
! AAATGCATCTTAAGAACATA+AGG + chr3_4:3947499-3947518 None:intergenic 25.0%
!!! TTATTTTTATTGCTGTGACT+TGG - chr3_4:3946290-3946309 Msa0486170:three_prime_UTR 25.0%
! ATATCACAGTAAAATATGAG+AGG + chr3_4:3946431-3946450 None:intergenic 25.0%
! CAGTTAAAAATATACTGAAC+TGG + chr3_4:3946574-3946593 None:intergenic 25.0%
! ATGTTACGATATTCTGATAT+AGG - chr3_4:3946644-3946663 Msa0486170:CDS 25.0%
!!! ATGAGATTGACTTTCATTTT+TGG + chr3_4:3946789-3946808 None:intergenic 25.0%
! AGTCAATCTCATGAAAATTT+AGG - chr3_4:3946797-3946816 Msa0486170:intron 25.0%
! GTCAATCTCATGAAAATTTA+GGG - chr3_4:3946798-3946817 Msa0486170:intron 25.0%
! TTATTATCATTACGAGCATA+TGG - chr3_4:3946841-3946860 Msa0486170:intron 25.0%
!!! CAAAAGATAAGTTTTATGGT+TGG + chr3_4:3946869-3946888 None:intergenic 25.0%
!! GAAGCAAAAGATAAGTTTTA+TGG + chr3_4:3946873-3946892 None:intergenic 25.0%
!! CCACTTCTTTATTCTTTTTA+AGG + chr3_4:3946934-3946953 None:intergenic 25.0%
! CCTTAAAAAGAATAAAGAAG+TGG - chr3_4:3946931-3946950 Msa0486170:intron 25.0%
! ACTAATATGTGATCTTACAA+TGG - chr3_4:3947084-3947103 Msa0486170:intron 25.0%
! GTTATATTGCAAAACAGAAA+TGG - chr3_4:3947426-3947445 Msa0486170:intron 25.0%
! AAATGCATCTTAAGAACATA+AGG + chr3_4:3947499-3947518 None:intergenic 25.0%
! AAATCTGTTTTGCAGAAAGA+AGG + chr3_4:3946037-3946056 None:intergenic 30.0%
TTTCTGCAAAACAGATTTCT+CGG - chr3_4:3946039-3946058 Msa0486170:three_prime_UTR 30.0%
TCTTGTTCATTATTGTACAC+AGG + chr3_4:3946674-3946693 None:intergenic 30.0%
TCTCTAATATGCAGAAAGAT+TGG + chr3_4:3946721-3946740 None:intergenic 30.0%
CTATGTTTCTCTTATAGATC+AGG - chr3_4:3946956-3946975 Msa0486170:intron 30.0%
TCTTATAGATCAGGACATAA+TGG - chr3_4:3946965-3946984 Msa0486170:intron 30.0%
AACAAAATGCTTGAGTTTAG+AGG - chr3_4:3947049-3947068 Msa0486170:intron 30.0%
GATCACATATTAGTAATCCA+AGG + chr3_4:3947078-3947097 None:intergenic 30.0%
!! ATATGCTTCAATTTGCTATG+TGG - chr3_4:3947111-3947130 Msa0486170:intron 30.0%
! AAGTTACAGTTTGTCCATTT+TGG - chr3_4:3947152-3947171 Msa0486170:intron 30.0%
! TAAGATTGTTGTTCGTTTCT+TGG - chr3_4:3947404-3947423 Msa0486170:intron 30.0%
TTCAGATATTGTGTAAGTCT+GGG + chr3_4:3947475-3947494 None:intergenic 30.0%
TTTCAGATATTGTGTAAGTC+TGG + chr3_4:3947476-3947495 None:intergenic 30.0%
!!! TGATTGTTGTTTTGGTTTCA+TGG - chr3_4:3948014-3948033 Msa0486170:five_prime_UTR 30.0%
!!! AAACAATGTTTTAGCATCAG+AGG + chr3_4:3948050-3948069 None:intergenic 30.0%
! AAATCTGTTTTGCAGAAAGA+AGG + chr3_4:3946037-3946056 None:intergenic 30.0%
TTTCTGCAAAACAGATTTCT+CGG - chr3_4:3946039-3946058 Msa0486170:three_prime_UTR 30.0%
TCTTGTTCATTATTGTACAC+AGG + chr3_4:3946674-3946693 None:intergenic 30.0%
TCTCTAATATGCAGAAAGAT+TGG + chr3_4:3946721-3946740 None:intergenic 30.0%
CTATGTTTCTCTTATAGATC+AGG - chr3_4:3946956-3946975 Msa0486170:intron 30.0%
TCTTATAGATCAGGACATAA+TGG - chr3_4:3946965-3946984 Msa0486170:intron 30.0%
AACAAAATGCTTGAGTTTAG+AGG - chr3_4:3947049-3947068 Msa0486170:intron 30.0%
GATCACATATTAGTAATCCA+AGG + chr3_4:3947078-3947097 None:intergenic 30.0%
!! ATATGCTTCAATTTGCTATG+TGG - chr3_4:3947111-3947130 Msa0486170:intron 30.0%
! AAGTTACAGTTTGTCCATTT+TGG - chr3_4:3947152-3947171 Msa0486170:intron 30.0%
! TAAGATTGTTGTTCGTTTCT+TGG - chr3_4:3947404-3947423 Msa0486170:intron 30.0%
TTCAGATATTGTGTAAGTCT+GGG + chr3_4:3947475-3947494 None:intergenic 30.0%
TTTCAGATATTGTGTAAGTC+TGG + chr3_4:3947476-3947495 None:intergenic 30.0%
!!! TGATTGTTGTTTTGGTTTCA+TGG - chr3_4:3948014-3948033 Msa0486170:five_prime_UTR 30.0%
!!! AAACAATGTTTTAGCATCAG+AGG + chr3_4:3948050-3948069 None:intergenic 30.0%
AATACAAATCTCAGAGCAGA+GGG + chr3_4:3946183-3946202 None:intergenic 35.0%
AAATACAAATCTCAGAGCAG+AGG + chr3_4:3946184-3946203 None:intergenic 35.0%
! TGCTGCATCTTTGAAAAAAG+GGG + chr3_4:3946326-3946345 None:intergenic 35.0%
! CTGCTGCATCTTTGAAAAAA+GGG + chr3_4:3946327-3946346 None:intergenic 35.0%
!!! CTTTTTTCAAAGATGCAGCA+GGG - chr3_4:3946326-3946345 Msa0486170:three_prime_UTR 35.0%
AAAAAGGGGATGCTATGTAT+TGG + chr3_4:3946357-3946376 None:intergenic 35.0%
TACTTTGATCAAACAGTTGG+CGG + chr3_4:3946506-3946525 None:intergenic 35.0%
GACTACTTTGATCAAACAGT+TGG + chr3_4:3946509-3946528 None:intergenic 35.0%
!!! TTTCATTTTTGGCTTCTCAC+TGG + chr3_4:3946778-3946797 None:intergenic 35.0%
CTAAGATTATGGCCTATGTA+GGG + chr3_4:3947214-3947233 None:intergenic 35.0%
TGCAAAACCACCTAAGATTA+TGG + chr3_4:3947225-3947244 None:intergenic 35.0%
TTGCATCATATTACCAGTCA+AGG - chr3_4:3947240-3947259 Msa0486170:intron 35.0%
GTATCATGGAAAGGTAAAAG+CGG - chr3_4:3947726-3947745 Msa0486170:intron 35.0%
AAAAATTACATGAGCAGCTC+AGG + chr3_4:3947913-3947932 None:intergenic 35.0%
ATGTTTGATGAGCAACTTGA+TGG + chr3_4:3947951-3947970 None:intergenic 35.0%
GATGCTAAAACATTGTTTCG+TGG - chr3_4:3948052-3948071 Msa0486170:five_prime_UTR 35.0%
AATACAAATCTCAGAGCAGA+GGG + chr3_4:3946183-3946202 None:intergenic 35.0%
AAATACAAATCTCAGAGCAG+AGG + chr3_4:3946184-3946203 None:intergenic 35.0%
! TGCTGCATCTTTGAAAAAAG+GGG + chr3_4:3946326-3946345 None:intergenic 35.0%
! CTGCTGCATCTTTGAAAAAA+GGG + chr3_4:3946327-3946346 None:intergenic 35.0%
!!! CTTTTTTCAAAGATGCAGCA+GGG - chr3_4:3946326-3946345 Msa0486170:three_prime_UTR 35.0%
AAAAAGGGGATGCTATGTAT+TGG + chr3_4:3946357-3946376 None:intergenic 35.0%
TACTTTGATCAAACAGTTGG+CGG + chr3_4:3946506-3946525 None:intergenic 35.0%
GACTACTTTGATCAAACAGT+TGG + chr3_4:3946509-3946528 None:intergenic 35.0%
!!! TTTCATTTTTGGCTTCTCAC+TGG + chr3_4:3946778-3946797 None:intergenic 35.0%
CTAAGATTATGGCCTATGTA+GGG + chr3_4:3947214-3947233 None:intergenic 35.0%
TGCAAAACCACCTAAGATTA+TGG + chr3_4:3947225-3947244 None:intergenic 35.0%
TTGCATCATATTACCAGTCA+AGG - chr3_4:3947240-3947259 Msa0486170:intron 35.0%
GTATCATGGAAAGGTAAAAG+CGG - chr3_4:3947726-3947745 Msa0486170:intron 35.0%
AAAAATTACATGAGCAGCTC+AGG + chr3_4:3947913-3947932 None:intergenic 35.0%
ATGTTTGATGAGCAACTTGA+TGG + chr3_4:3947951-3947970 None:intergenic 35.0%
GATGCTAAAACATTGTTTCG+TGG - chr3_4:3948052-3948071 Msa0486170:five_prime_UTR 35.0%
ATGTTCGAACCTGTTTGTGA+CGG + chr3_4:3946159-3946178 None:intergenic 40.0%
! GCTGCATCTTTGAAAAAAGG+GGG + chr3_4:3946325-3946344 None:intergenic 40.0%
! CCTGCTGCATCTTTGAAAAA+AGG + chr3_4:3946328-3946347 None:intergenic 40.0%
!!! CCTTTTTTCAAAGATGCAGC+AGG - chr3_4:3946325-3946344 Msa0486170:three_prime_UTR 40.0%
ACTATCAGTGGATACAGCAA+GGG + chr3_4:3946464-3946483 None:intergenic 40.0%
AACTATCAGTGGATACAGCA+AGG + chr3_4:3946465-3946484 None:intergenic 40.0%
GCTGTATCCACTGATAGTTT+CGG - chr3_4:3946466-3946485 Msa0486170:CDS 40.0%
CTGTATCCACTGATAGTTTC+GGG - chr3_4:3946467-3946486 Msa0486170:CDS 40.0%
TGTATCCACTGATAGTTTCG+GGG - chr3_4:3946468-3946487 Msa0486170:CDS 40.0%
!!! TGTTTTGGCTTCAAGTTCCT+GGG - chr3_4:3946592-3946611 Msa0486170:CDS 40.0%
!! TCAGGACATAATGGACATAC+TGG - chr3_4:3946974-3946993 Msa0486170:intron 40.0%
TTGAAAGAGATGCCCTACAT+AGG - chr3_4:3947199-3947218 Msa0486170:intron 40.0%
CCTAAGATTATGGCCTATGT+AGG + chr3_4:3947215-3947234 None:intergenic 40.0%
CCTACATAGGCCATAATCTT+AGG - chr3_4:3947212-3947231 Msa0486170:intron 40.0%
ACATAGGCCATAATCTTAGG+TGG - chr3_4:3947215-3947234 Msa0486170:intron 40.0%
CAGTCAAGGAATGTTGAAAG+CGG - chr3_4:3947254-3947273 Msa0486170:intron 40.0%
AGATATTGTGTAAGTCTGGG+AGG + chr3_4:3947472-3947491 None:intergenic 40.0%
AAAGATTGGGAAGCTACTAG+CGG - chr3_4:3947645-3947664 Msa0486170:intron 40.0%
TTCAAGTGAACAGAAACAGC+AGG + chr3_4:3947694-3947713 None:intergenic 40.0%
TTCACTTGAAGAGAGAACAG+AGG - chr3_4:3947704-3947723 Msa0486170:intron 40.0%
!! AAGAGAGAACAGAGGTATCA+TGG - chr3_4:3947712-3947731 Msa0486170:intron 40.0%
!! AGAACAGAGGTATCATGGAA+AGG - chr3_4:3947717-3947736 Msa0486170:intron 40.0%
!! AAGCGGTTATTGATTCTCTC+AGG - chr3_4:3947743-3947762 Msa0486170:intron 40.0%
!! AGCGGTTATTGATTCTCTCA+GGG - chr3_4:3947744-3947763 Msa0486170:intron 40.0%
ATGAGCAGCTCAGGTTTAAT+TGG + chr3_4:3947904-3947923 None:intergenic 40.0%
!! GTTTTAGCATCAGAGGTAGA+GGG + chr3_4:3948043-3948062 None:intergenic 40.0%
!!! TGTTTTAGCATCAGAGGTAG+AGG + chr3_4:3948044-3948063 None:intergenic 40.0%
! TGGTTTGCAGAGTTGCAAAT+TGG - chr3_4:3948085-3948104 Msa0486170:five_prime_UTR 40.0%
TGCAGAGTTGCAAATTGGTA+AGG - chr3_4:3948090-3948109 Msa0486170:five_prime_UTR 40.0%
CCAAAACACCATACACTAGT+CGG + chr3_4:3948138-3948157 None:intergenic 40.0%
ATGTTCGAACCTGTTTGTGA+CGG + chr3_4:3946159-3946178 None:intergenic 40.0%
! GCTGCATCTTTGAAAAAAGG+GGG + chr3_4:3946325-3946344 None:intergenic 40.0%
! CCTGCTGCATCTTTGAAAAA+AGG + chr3_4:3946328-3946347 None:intergenic 40.0%
!!! CCTTTTTTCAAAGATGCAGC+AGG - chr3_4:3946325-3946344 Msa0486170:three_prime_UTR 40.0%
ACTATCAGTGGATACAGCAA+GGG + chr3_4:3946464-3946483 None:intergenic 40.0%
AACTATCAGTGGATACAGCA+AGG + chr3_4:3946465-3946484 None:intergenic 40.0%
GCTGTATCCACTGATAGTTT+CGG - chr3_4:3946466-3946485 Msa0486170:CDS 40.0%
CTGTATCCACTGATAGTTTC+GGG - chr3_4:3946467-3946486 Msa0486170:CDS 40.0%
TGTATCCACTGATAGTTTCG+GGG - chr3_4:3946468-3946487 Msa0486170:CDS 40.0%
!!! TGTTTTGGCTTCAAGTTCCT+GGG - chr3_4:3946592-3946611 Msa0486170:CDS 40.0%
!! TCAGGACATAATGGACATAC+TGG - chr3_4:3946974-3946993 Msa0486170:intron 40.0%
TTGAAAGAGATGCCCTACAT+AGG - chr3_4:3947199-3947218 Msa0486170:intron 40.0%
CCTAAGATTATGGCCTATGT+AGG + chr3_4:3947215-3947234 None:intergenic 40.0%
CCTACATAGGCCATAATCTT+AGG - chr3_4:3947212-3947231 Msa0486170:intron 40.0%
ACATAGGCCATAATCTTAGG+TGG - chr3_4:3947215-3947234 Msa0486170:intron 40.0%
CAGTCAAGGAATGTTGAAAG+CGG - chr3_4:3947254-3947273 Msa0486170:intron 40.0%
AGATATTGTGTAAGTCTGGG+AGG + chr3_4:3947472-3947491 None:intergenic 40.0%
AAAGATTGGGAAGCTACTAG+CGG - chr3_4:3947645-3947664 Msa0486170:intron 40.0%
TTCAAGTGAACAGAAACAGC+AGG + chr3_4:3947694-3947713 None:intergenic 40.0%
TTCACTTGAAGAGAGAACAG+AGG - chr3_4:3947704-3947723 Msa0486170:intron 40.0%
!! AAGAGAGAACAGAGGTATCA+TGG - chr3_4:3947712-3947731 Msa0486170:intron 40.0%
!! AGAACAGAGGTATCATGGAA+AGG - chr3_4:3947717-3947736 Msa0486170:intron 40.0%
!! AAGCGGTTATTGATTCTCTC+AGG - chr3_4:3947743-3947762 Msa0486170:intron 40.0%
!! AGCGGTTATTGATTCTCTCA+GGG - chr3_4:3947744-3947763 Msa0486170:intron 40.0%
ATGAGCAGCTCAGGTTTAAT+TGG + chr3_4:3947904-3947923 None:intergenic 40.0%
!! GTTTTAGCATCAGAGGTAGA+GGG + chr3_4:3948043-3948062 None:intergenic 40.0%
!!! TGTTTTAGCATCAGAGGTAG+AGG + chr3_4:3948044-3948063 None:intergenic 40.0%
! TGGTTTGCAGAGTTGCAAAT+TGG - chr3_4:3948085-3948104 Msa0486170:five_prime_UTR 40.0%
TGCAGAGTTGCAAATTGGTA+AGG - chr3_4:3948090-3948109 Msa0486170:five_prime_UTR 40.0%
CCAAAACACCATACACTAGT+CGG + chr3_4:3948138-3948157 None:intergenic 40.0%
!!! CTGTTTTGGCTTCAAGTTCC+TGG - chr3_4:3946591-3946610 Msa0486170:CDS 45.0%
!!! GTTTTGGCTTCAAGTTCCTG+GGG - chr3_4:3946593-3946612 Msa0486170:CDS 45.0%
AATAACACTTGGAACACCCC+AGG + chr3_4:3946612-3946631 None:intergenic 45.0%
TGGACATACTGGTAGAAACG+AGG - chr3_4:3946985-3947004 Msa0486170:intron 45.0%
CTTGAGTTTAGAGGAAGCCT+TGG - chr3_4:3947058-3947077 Msa0486170:intron 45.0%
CGCTTTCAACATTCCTTGAC+TGG + chr3_4:3947256-3947275 None:intergenic 45.0%
AGTTTCCAGGCTTGATCTCA+AGG + chr3_4:3947602-3947621 None:intergenic 45.0%
GATCAAGCCTGGAAACTACT+CGG - chr3_4:3947605-3947624 Msa0486170:intron 45.0%
ATCAAGCCTGGAAACTACTC+GGG - chr3_4:3947606-3947625 Msa0486170:intron 45.0%
TTGCTGCAGCTGCAAAGATT+GGG - chr3_4:3947632-3947651 Msa0486170:intron 45.0%
! ATTGATTCTCTCAGGGAAGC+TGG - chr3_4:3947751-3947770 Msa0486170:intron 45.0%
AAACACCATACACTAGTCGG+TGG + chr3_4:3948135-3948154 None:intergenic 45.0%
! CCGACTAGTGTATGGTGTTT+TGG - chr3_4:3948135-3948154 Msa0486170:five_prime_UTR 45.0%
!!! CTGTTTTGGCTTCAAGTTCC+TGG - chr3_4:3946591-3946610 Msa0486170:CDS 45.0%
!!! GTTTTGGCTTCAAGTTCCTG+GGG - chr3_4:3946593-3946612 Msa0486170:CDS 45.0%
AATAACACTTGGAACACCCC+AGG + chr3_4:3946612-3946631 None:intergenic 45.0%
TGGACATACTGGTAGAAACG+AGG - chr3_4:3946985-3947004 Msa0486170:intron 45.0%
CTTGAGTTTAGAGGAAGCCT+TGG - chr3_4:3947058-3947077 Msa0486170:intron 45.0%
CGCTTTCAACATTCCTTGAC+TGG + chr3_4:3947256-3947275 None:intergenic 45.0%
AGTTTCCAGGCTTGATCTCA+AGG + chr3_4:3947602-3947621 None:intergenic 45.0%
GATCAAGCCTGGAAACTACT+CGG - chr3_4:3947605-3947624 Msa0486170:intron 45.0%
ATCAAGCCTGGAAACTACTC+GGG - chr3_4:3947606-3947625 Msa0486170:intron 45.0%
TTGCTGCAGCTGCAAAGATT+GGG - chr3_4:3947632-3947651 Msa0486170:intron 45.0%
! ATTGATTCTCTCAGGGAAGC+TGG - chr3_4:3947751-3947770 Msa0486170:intron 45.0%
AAACACCATACACTAGTCGG+TGG + chr3_4:3948135-3948154 None:intergenic 45.0%
! CCGACTAGTGTATGGTGTTT+TGG - chr3_4:3948135-3948154 Msa0486170:five_prime_UTR 45.0%
GCACGAGAGAAAGAGCAGTA+GGG + chr3_4:3946073-3946092 None:intergenic 50.0%
TGCACGAGAGAAAGAGCAGT+AGG + chr3_4:3946074-3946093 None:intergenic 50.0%
AGAAAGGAACGGGATGGGAA+GGG + chr3_4:3946134-3946153 None:intergenic 50.0%
TTGTGACGGCGAGAAAGGAA+CGG + chr3_4:3946145-3946164 None:intergenic 50.0%
TCAGTGGATACAGCAAGGGT+GGG + chr3_4:3946460-3946479 None:intergenic 50.0%
ATCAGTGGATACAGCAAGGG+TGG + chr3_4:3946461-3946480 None:intergenic 50.0%
CTATGCCCCGAAACTATCAG+TGG + chr3_4:3946476-3946495 None:intergenic 50.0%
ACTGGTAGAAACGAGGTGTG+AGG - chr3_4:3946992-3947011 Msa0486170:intron 50.0%
AGAAACGAGGTGTGAGGATG+AGG - chr3_4:3946998-3947017 Msa0486170:intron 50.0%
TGAGCTTGCAGAAGAGGCAA+CGG + chr3_4:3947569-3947588 None:intergenic 50.0%
GAAAGCCTTGAGATCAAGCC+TGG - chr3_4:3947594-3947613 Msa0486170:intron 50.0%
CAACATCCCGAGTAGTTTCC+AGG + chr3_4:3947615-3947634 None:intergenic 50.0%
GTTGCTGCAGCTGCAAAGAT+TGG - chr3_4:3947631-3947650 Msa0486170:intron 50.0%
CAGGAATGTCCTTGAGCGAA+AGG + chr3_4:3947675-3947694 None:intergenic 50.0%
TTTGATCACTCGTTCGCAGG+AGG + chr3_4:3947848-3947867 None:intergenic 50.0%
TCCTGCGAACGAGTGATCAA+AGG - chr3_4:3947847-3947866 Msa0486170:intron 50.0%
ACCTTTGATCACTCGTTCGC+AGG + chr3_4:3947851-3947870 None:intergenic 50.0%
TGTTTCGTGGCAAGCGTAAG+TGG - chr3_4:3948065-3948084 Msa0486170:five_prime_UTR 50.0%
GCACGAGAGAAAGAGCAGTA+GGG + chr3_4:3946073-3946092 None:intergenic 50.0%
TGCACGAGAGAAAGAGCAGT+AGG + chr3_4:3946074-3946093 None:intergenic 50.0%
AGAAAGGAACGGGATGGGAA+GGG + chr3_4:3946134-3946153 None:intergenic 50.0%
TTGTGACGGCGAGAAAGGAA+CGG + chr3_4:3946145-3946164 None:intergenic 50.0%
TCAGTGGATACAGCAAGGGT+GGG + chr3_4:3946460-3946479 None:intergenic 50.0%
ATCAGTGGATACAGCAAGGG+TGG + chr3_4:3946461-3946480 None:intergenic 50.0%
CTATGCCCCGAAACTATCAG+TGG + chr3_4:3946476-3946495 None:intergenic 50.0%
ACTGGTAGAAACGAGGTGTG+AGG - chr3_4:3946992-3947011 Msa0486170:intron 50.0%
AGAAACGAGGTGTGAGGATG+AGG - chr3_4:3946998-3947017 Msa0486170:intron 50.0%
TGAGCTTGCAGAAGAGGCAA+CGG + chr3_4:3947569-3947588 None:intergenic 50.0%
GAAAGCCTTGAGATCAAGCC+TGG - chr3_4:3947594-3947613 Msa0486170:intron 50.0%
CAACATCCCGAGTAGTTTCC+AGG + chr3_4:3947615-3947634 None:intergenic 50.0%
GTTGCTGCAGCTGCAAAGAT+TGG - chr3_4:3947631-3947650 Msa0486170:intron 50.0%
CAGGAATGTCCTTGAGCGAA+AGG + chr3_4:3947675-3947694 None:intergenic 50.0%
TTTGATCACTCGTTCGCAGG+AGG + chr3_4:3947848-3947867 None:intergenic 50.0%
TCCTGCGAACGAGTGATCAA+AGG - chr3_4:3947847-3947866 Msa0486170:intron 50.0%
ACCTTTGATCACTCGTTCGC+AGG + chr3_4:3947851-3947870 None:intergenic 50.0%
TGTTTCGTGGCAAGCGTAAG+TGG - chr3_4:3948065-3948084 Msa0486170:five_prime_UTR 50.0%
GAGAAAGGAACGGGATGGGA+AGG + chr3_4:3946135-3946154 None:intergenic 55.0%
TGTGACGGCGAGAAAGGAAC+GGG + chr3_4:3946144-3946163 None:intergenic 55.0%
CCTGTTTGTGACGGCGAGAA+AGG + chr3_4:3946150-3946169 None:intergenic 55.0%
CCTTTCTCGCCGTCACAAAC+AGG - chr3_4:3946147-3946166 Msa0486170:three_prime_UTR 55.0%
TGGTTATCCCACCTCCAGTC+AGG - chr3_4:3947446-3947465 Msa0486170:intron 55.0%
CAACGGTAGCAGTTGGAGTG+TGG + chr3_4:3947552-3947571 None:intergenic 55.0%
GAAGAGGCAACGGTAGCAGT+TGG + chr3_4:3947559-3947578 None:intergenic 55.0%
TGCCTCTTCTGCAAGCTCAC+AGG - chr3_4:3947570-3947589 Msa0486170:intron 55.0%
CTCCTGTGAGCTTGCAGAAG+AGG + chr3_4:3947575-3947594 None:intergenic 55.0%
GCAGGAGGCAAGGTTCAACA+AGG + chr3_4:3947833-3947852 None:intergenic 55.0%
! GACTGCCACCGACTAGTGTA+TGG - chr3_4:3948127-3948146 Msa0486170:five_prime_UTR 55.0%
GAGAAAGGAACGGGATGGGA+AGG + chr3_4:3946135-3946154 None:intergenic 55.0%
TGTGACGGCGAGAAAGGAAC+GGG + chr3_4:3946144-3946163 None:intergenic 55.0%
CCTGTTTGTGACGGCGAGAA+AGG + chr3_4:3946150-3946169 None:intergenic 55.0%
CCTTTCTCGCCGTCACAAAC+AGG - chr3_4:3946147-3946166 Msa0486170:three_prime_UTR 55.0%
TGGTTATCCCACCTCCAGTC+AGG - chr3_4:3947446-3947465 Msa0486170:intron 55.0%
CAACGGTAGCAGTTGGAGTG+TGG + chr3_4:3947552-3947571 None:intergenic 55.0%
GAAGAGGCAACGGTAGCAGT+TGG + chr3_4:3947559-3947578 None:intergenic 55.0%
TGCCTCTTCTGCAAGCTCAC+AGG - chr3_4:3947570-3947589 Msa0486170:intron 55.0%
CTCCTGTGAGCTTGCAGAAG+AGG + chr3_4:3947575-3947594 None:intergenic 55.0%
GCAGGAGGCAAGGTTCAACA+AGG + chr3_4:3947833-3947852 None:intergenic 55.0%
! GACTGCCACCGACTAGTGTA+TGG - chr3_4:3948127-3948146 Msa0486170:five_prime_UTR 55.0%
ATGGGAAGGGAGTGGCTGAC+CGG + chr3_4:3946121-3946140 None:intergenic 60.0%
CGGCGAGAAAGGAACGGGAT+GGG + chr3_4:3946139-3946158 None:intergenic 60.0%
ACGGCGAGAAAGGAACGGGA+TGG + chr3_4:3946140-3946159 None:intergenic 60.0%
GTAAGTCTGGGAGGCCTGAC+TGG + chr3_4:3947463-3947482 None:intergenic 60.0%
AGCGGAACGCCTTTCGCTCA+AGG - chr3_4:3947663-3947682 Msa0486170:intron 60.0%
TCACTCGTTCGCAGGAGGCA+AGG + chr3_4:3947843-3947862 None:intergenic 60.0%
ATGGGAAGGGAGTGGCTGAC+CGG + chr3_4:3946121-3946140 None:intergenic 60.0%
CGGCGAGAAAGGAACGGGAT+GGG + chr3_4:3946139-3946158 None:intergenic 60.0%
ACGGCGAGAAAGGAACGGGA+TGG + chr3_4:3946140-3946159 None:intergenic 60.0%
GTAAGTCTGGGAGGCCTGAC+TGG + chr3_4:3947463-3947482 None:intergenic 60.0%
AGCGGAACGCCTTTCGCTCA+AGG - chr3_4:3947663-3947682 Msa0486170:intron 60.0%
TCACTCGTTCGCAGGAGGCA+AGG + chr3_4:3947843-3947862 None:intergenic 60.0%
GCTGACCGGAGTGGACGTGA+CGG + chr3_4:3946107-3946126 None:intergenic 65.0%
AAGGGAGTGGCTGACCGGAG+TGG + chr3_4:3946116-3946135 None:intergenic 65.0%
GGAACGGGATGGGAAGGGAG+TGG + chr3_4:3946129-3946148 None:intergenic 65.0%
TGGGAGGCCTGACTGGAGGT+GGG + chr3_4:3947456-3947475 None:intergenic 65.0%
AGTCTGGGAGGCCTGACTGG+AGG + chr3_4:3947460-3947479 None:intergenic 65.0%
GCTGACCGGAGTGGACGTGA+CGG + chr3_4:3946107-3946126 None:intergenic 65.0%
AAGGGAGTGGCTGACCGGAG+TGG + chr3_4:3946116-3946135 None:intergenic 65.0%
GGAACGGGATGGGAAGGGAG+TGG + chr3_4:3946129-3946148 None:intergenic 65.0%
TGGGAGGCCTGACTGGAGGT+GGG + chr3_4:3947456-3947475 None:intergenic 65.0%
AGTCTGGGAGGCCTGACTGG+AGG + chr3_4:3947460-3947479 None:intergenic 65.0%
GACCGGAGTGGACGTGACGG+CGG + chr3_4:3946104-3946123 None:intergenic 70.0%
CTGGGAGGCCTGACTGGAGG+TGG + chr3_4:3947457-3947476 None:intergenic 70.0%
GACCGGAGTGGACGTGACGG+CGG + chr3_4:3946104-3946123 None:intergenic 70.0%
CTGGGAGGCCTGACTGGAGG+TGG + chr3_4:3947457-3947476 None:intergenic 70.0%
CGGAGTGGACGTGACGGCGG+CGG + chr3_4:3946101-3946120 None:intergenic 75.0%
CGCCGCCGTCACGTCCACTC+CGG - chr3_4:3946099-3946118 Msa0486170:three_prime_UTR 75.0%
CGGAGTGGACGTGACGGCGG+CGG + chr3_4:3946101-3946120 None:intergenic 75.0%
CGCCGCCGTCACGTCCACTC+CGG - chr3_4:3946099-3946118 Msa0486170:three_prime_UTR 75.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 3945997 3948159 3945997 ID=Msa0486170;Name=Msa0486170
chr3_4 mRNA 3945997 3948159 3945997 ID=Msa0486170-mRNA-1;Parent=Msa0486170;Name=Msa0486170-mRNA-1;_AED=0.02;_eAED=0.02;_QI=174|1|1|1|0|0.5|2|370|114
chr3_4 exon 3945997 3946713 3945997 ID=Msa0486170-mRNA-1:exon:1537;Parent=Msa0486170-mRNA-1
chr3_4 exon 3947988 3948159 3947988 ID=Msa0486170-mRNA-1:exon:1536;Parent=Msa0486170-mRNA-1
chr3_4 five_prime_UTR 3947988 3948159 3947988 ID=Msa0486170-mRNA-1:five_prime_utr;Parent=Msa0486170-mRNA-1
chr3_4 five_prime_UTR 3946712 3946713 3946712 ID=Msa0486170-mRNA-1:five_prime_utr;Parent=Msa0486170-mRNA-1
chr3_4 CDS 3946367 3946711 3946367 ID=Msa0486170-mRNA-1:cds;Parent=Msa0486170-mRNA-1
chr3_4 three_prime_UTR 3945997 3946366 3945997 ID=Msa0486170-mRNA-1:three_prime_utr;Parent=Msa0486170-mRNA-1
Gene Sequence

>Msa0486170

ATGGTTATCCCACCTCCAGTCAGGCCTCCCAGACTTACACAATATCTGAAACCTTATGTTCTTAAGATGCATTTCACAAATAAGTATGTGAGTGCTCAAGTGATCCACACTCCAACTGCTACCGTTGCCTCTTCTGCAAGCTCACAGGAGAAAGCCTTGAGATCAAGCCTGGAAACTACTCGGGATGTTGCTGCAGCTGCAAAGATTGGGAAGCTACTAGCGGAACGCCTTTCGCTCAAGGACATTCCTGCTGTTTCTGTTCACTTGAAGAGAGAACAGAGGTATCATGGAAAGGTAAAAGCGGTTATTGATTCTCTCAGGGAAGCTGGTGTTAAGTTGCTTTGA
Protein sequence

>Msa0486170

MVIPPPVRPPRLTQYLKPYVLKMHFTNKYVSAQVIHTPTATVASSASSQEKALRSSLETTRDVAAAAKIGKLLAERLSLKDIPAVSVHLKREQRYHGKVKAVIDSLREAGVKLL*