AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0504450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0504450 MtrunA17_Chr3g0104681 95.858 169 7 0 4 172 363 531 1.50e-106 314
Msa0504450 MtrunA17_Chr3g0093341 57.229 166 67 4 4 167 212 375 1.54e-53 174
Msa0504450 MtrunA17_Chr3g0132121 55.625 160 69 2 4 162 358 516 5.34e-53 176
Msa0504450 MtrunA17_Chr8g0338041 54.483 145 62 4 7 150 98 239 9.75e-43 143
Msa0504450 MtrunA17_Chr8g0337991 51.299 154 71 4 7 159 355 505 9.28e-40 140
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0504450 AT1G07230.1 74.706 170 42 1 4 172 364 533 1.10e-87 266
Msa0504450 AT3G48610.1 59.873 157 61 2 4 159 362 517 2.91e-55 182
Msa0504450 AT2G26870.1 59.748 159 59 4 4 159 356 512 9.93e-53 175
Msa0504450 AT3G03520.1 49.664 149 70 4 7 152 352 498 3.03e-37 134
Msa0504450 AT3G03530.1 48.592 142 69 4 7 147 352 490 1.89e-33 124
Msa0504450 AT3G03540.1 47.945 146 70 5 7 150 351 492 1.24e-30 116
Msa0504450 AT3G03520.2 67.797 59 17 2 7 65 352 408 6.90e-19 83.2

Find 49 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGCAACTTATGAAAATT+AGG 0.131738 3_4:-48912396 None:intergenic
CTTATGAAAATTAGGAATTT+AGG 0.156688 3_4:-48912388 Msa0504450:intron
ATCTCTCTTTGTTAGGAAAT+TGG 0.200119 3_4:+48911840 None:intergenic
CTCAATTCCTATCCAAATAT+TGG 0.206289 3_4:-48910779 Msa0504450:CDS
GAGAGTCTTGAATCTTCTTT+TGG 0.230691 3_4:+48910857 None:intergenic
ATTTACGAAATTAAAATTCT+TGG 0.233491 3_4:+48910340 None:intergenic
GTAAGATTCCAACTGCTTTC+GGG 0.237675 3_4:+48910601 None:intergenic
AGTAAGATTCCAACTGCTTT+CGG 0.261842 3_4:+48910600 None:intergenic
CTTAGATTATTCTTGACTTC+AGG 0.330762 3_4:+48910890 None:intergenic
GAATGTTCATATTGAGAATA+TGG 0.343966 3_4:+48911904 None:intergenic
ACCAGACAATTGATTAGTTA+TGG 0.366429 3_4:-48910439 Msa0504450:three_prime_UTR
AAGAATAATCTAAGGCCATT+TGG 0.366711 3_4:-48910881 Msa0504450:CDS
TCAATTCCTATCCAAATATT+GGG 0.372979 3_4:-48910778 Msa0504450:CDS
TACTGCTTTGTTTGAGAAGA+TGG 0.374570 3_4:+48910265 None:intergenic
AAAAGCTTCTTTACTGTGGC+AGG 0.376138 3_4:+48911874 None:intergenic
TATCTTTCTTAAATATCATA+TGG 0.386565 3_4:+48910506 None:intergenic
ACCATAACTAATCAATTGTC+TGG 0.397346 3_4:+48910438 None:intergenic
CCCATGCATCTCTCTTTGTT+AGG 0.398458 3_4:+48911833 None:intergenic
TCTTGCAGTGATTCATGAAC+CGG 0.398754 3_4:-48911938 Msa0504450:intron
CAACAGAAACTCTGCTGGTA+AGG 0.399781 3_4:+48910630 None:intergenic
CTGAAGTCAAGAATAATCTA+AGG 0.408667 3_4:-48910889 Msa0504450:CDS
ACCAGCAGAGTTTCTGTTGG+AGG 0.410016 3_4:-48910626 Msa0504450:CDS
CGTTGAGCTGTGATGCTAGC+TGG 0.446038 3_4:+48910813 None:intergenic
GAATATGGCGTTGGACCATC+CGG 0.456975 3_4:+48911919 None:intergenic
GTGAAGAGATTTCTTGAAGC+AGG 0.476264 3_4:-48910710 Msa0504450:CDS
CTTACCAGCAGAGTTTCTGT+TGG 0.489906 3_4:-48910629 Msa0504450:CDS
GGAAAGGCTGCTCTTAAAGC+TGG 0.495046 3_4:-48910689 Msa0504450:CDS
ACTCCTCGTGATGATTGTCC+AGG 0.499421 3_4:-48911775 Msa0504450:intron
ATTTAAGAAAGATATACTAA+TGG 0.518748 3_4:-48910497 Msa0504450:three_prime_UTR
ACTAATTTATTCACAACTAG+AGG 0.545914 3_4:-48910580 Msa0504450:exon
TGGAGGTTCCCGAAAGCAGT+TGG 0.546637 3_4:-48910609 Msa0504450:CDS
GAGATTTCTTGAAGCAGGAA+AGG 0.547450 3_4:-48910705 Msa0504450:CDS
GCTTTGTTTGAGAAGATGGA+TGG 0.550135 3_4:+48910269 None:intergenic
AACAGAAACTCTGCTGGTAA+GGG 0.558897 3_4:+48910631 None:intergenic
TCATATTGAGAATATGGCGT+TGG 0.561607 3_4:+48911910 None:intergenic
ACCTCCAACAGAAACTCTGC+TGG 0.564939 3_4:+48910625 None:intergenic
ACAAAGAGAGATGCATGGGC+TGG 0.583222 3_4:-48911829 Msa0504450:CDS
CCTAACAAAGAGAGATGCAT+GGG 0.585846 3_4:-48911833 Msa0504450:CDS
ATTGAAAAGCTTCTTTACTG+TGG 0.601890 3_4:+48911870 None:intergenic
GAAACTCTGCTGGTAAGGGA+AGG 0.611765 3_4:+48910635 None:intergenic
TCCTAACAAAGAGAGATGCA+TGG 0.617737 3_4:-48911834 Msa0504450:CDS
AATGTAAAAGTACTTCTCGA+AGG 0.621722 3_4:+48911804 None:intergenic
CAGCTAGCATCACAGCTCAA+CGG 0.636071 3_4:-48910812 Msa0504450:CDS
GCAGTGATTCATGAACCGGA+TGG 0.689986 3_4:-48911934 Msa0504450:intron
ACTCTGCTATTGTCACAATG+AGG 0.691650 3_4:-48910658 Msa0504450:CDS
CTTGATTAATCACACCGTGG+TGG 0.712865 3_4:-48910392 Msa0504450:three_prime_UTR
AAACCTGGACAATCATCACG+AGG 0.748499 3_4:+48911772 None:intergenic
GGACTTGATTAATCACACCG+TGG 0.755445 3_4:-48910395 Msa0504450:three_prime_UTR
TTAAGAACAATCAACCACCA+CGG 0.764175 3_4:+48910378 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATTGATACAAATATATT+TGG + chr3_4:48911023-48911042 None:intergenic 10.0%
!! AATATATTTGTATCAATATT+TGG - chr3_4:48911023-48911042 Msa0504450:intron 10.0%
!! AATATTGATACAAATATATT+TGG + chr3_4:48911023-48911042 None:intergenic 10.0%
!! AATATATTTGTATCAATATT+TGG - chr3_4:48911023-48911042 Msa0504450:intron 10.0%
!!! AAAAAAAATTTTGGTTAGTA+AGG + chr3_4:48910605-48910624 None:intergenic 15.0%
!! TATCTTTCTTAAATATCATA+TGG + chr3_4:48912149-48912168 None:intergenic 15.0%
!! ATTTAAGAAAGATATACTAA+TGG - chr3_4:48912155-48912174 Msa0504450:intron 15.0%
!! TTATGGTAAAAAAGATTATT+TGG - chr3_4:48912230-48912249 Msa0504450:intron 15.0%
!! TAAAAAAGATTATTTGGTTA+TGG - chr3_4:48912236-48912255 Msa0504450:intron 15.0%
!! ATTTACGAAATTAAAATTCT+TGG + chr3_4:48912315-48912334 None:intergenic 15.0%
!!! AAAAAAAATTTTGGTTAGTA+AGG + chr3_4:48910605-48910624 None:intergenic 15.0%
!! TATCTTTCTTAAATATCATA+TGG + chr3_4:48912149-48912168 None:intergenic 15.0%
!! ATTTAAGAAAGATATACTAA+TGG - chr3_4:48912155-48912174 Msa0504450:intron 15.0%
!! TTATGGTAAAAAAGATTATT+TGG - chr3_4:48912230-48912249 Msa0504450:intron 15.0%
!! TAAAAAAGATTATTTGGTTA+TGG - chr3_4:48912236-48912255 Msa0504450:intron 15.0%
!! ATTTACGAAATTAAAATTCT+TGG + chr3_4:48912315-48912334 None:intergenic 15.0%
!! TTCCTGTATTTCAAAAATTA+TGG - chr3_4:48910428-48910447 Msa0504450:three_prime_UTR 20.0%
!!! AACCATAATTTTTGAAATAC+AGG + chr3_4:48910433-48910452 None:intergenic 20.0%
!! TTTCAAAAATTATGGTTGAT+TGG - chr3_4:48910436-48910455 Msa0504450:three_prime_UTR 20.0%
!!! TTTTTTTATTATTTCTGTGC+CGG - chr3_4:48910619-48910638 Msa0504450:CDS 20.0%
!! ACATTTGACTAAATAAATCT+TGG - chr3_4:48910924-48910943 Msa0504450:intron 20.0%
!!! AGAGAATGTTACTTTTATTA+GGG + chr3_4:48910995-48911014 None:intergenic 20.0%
!! CTATATTGGAATTATTATCA+TGG + chr3_4:48911399-48911418 None:intergenic 20.0%
!! CATGGAATTATTATCTATAT+TGG + chr3_4:48911413-48911432 None:intergenic 20.0%
!! CTATATTGGAATTATTATCA+TGG + chr3_4:48911399-48911418 None:intergenic 20.0%
!! CATGGAATTATTATCTATAT+TGG + chr3_4:48911413-48911432 None:intergenic 20.0%
!! CTATATTGGAATTATTATCA+TGG + chr3_4:48911399-48911418 None:intergenic 20.0%
!!! ACAAAGTTTTGTACATATTT+TGG + chr3_4:48911647-48911666 None:intergenic 20.0%
!! AATAAATCAATTGCATCAAA+CGG + chr3_4:48911691-48911710 None:intergenic 20.0%
!!! ATAATGTTAATTGATGATGT+TGG + chr3_4:48911716-48911735 None:intergenic 20.0%
!! TTCCTGTATTTCAAAAATTA+TGG - chr3_4:48910428-48910447 Msa0504450:three_prime_UTR 20.0%
!!! AACCATAATTTTTGAAATAC+AGG + chr3_4:48910433-48910452 None:intergenic 20.0%
!! TTTCAAAAATTATGGTTGAT+TGG - chr3_4:48910436-48910455 Msa0504450:three_prime_UTR 20.0%
!!! TTTTTTTATTATTTCTGTGC+CGG - chr3_4:48910619-48910638 Msa0504450:CDS 20.0%
!! ACATTTGACTAAATAAATCT+TGG - chr3_4:48910924-48910943 Msa0504450:intron 20.0%
!!! AGAGAATGTTACTTTTATTA+GGG + chr3_4:48910995-48911014 None:intergenic 20.0%
!! CTATATTGGAATTATTATCA+TGG + chr3_4:48911399-48911418 None:intergenic 20.0%
!! CATGGAATTATTATCTATAT+TGG + chr3_4:48911413-48911432 None:intergenic 20.0%
!! CTATATTGGAATTATTATCA+TGG + chr3_4:48911399-48911418 None:intergenic 20.0%
!! CATGGAATTATTATCTATAT+TGG + chr3_4:48911413-48911432 None:intergenic 20.0%
!! CTATATTGGAATTATTATCA+TGG + chr3_4:48911399-48911418 None:intergenic 20.0%
!!! ACAAAGTTTTGTACATATTT+TGG + chr3_4:48911647-48911666 None:intergenic 20.0%
!! AATAAATCAATTGCATCAAA+CGG + chr3_4:48911691-48911710 None:intergenic 20.0%
!!! ATAATGTTAATTGATGATGT+TGG + chr3_4:48911716-48911735 None:intergenic 20.0%
! GAATGTTCATATTGAGAATA+TGG + chr3_4:48910751-48910770 None:intergenic 25.0%
!!! GAGAATGTTACTTTTATTAG+GGG + chr3_4:48910994-48911013 None:intergenic 25.0%
!!! CAGAGAATGTTACTTTTATT+AGG + chr3_4:48910996-48911015 None:intergenic 25.0%
! AATCTGTATGACACAATAAA+TGG - chr3_4:48911354-48911373 Msa0504450:intron 25.0%
! ATTGGAATTATTATCATGGA+TGG + chr3_4:48911427-48911446 None:intergenic 25.0%
! TCAATTCCTATCCAAATATT+GGG - chr3_4:48911874-48911893 Msa0504450:CDS 25.0%
! ACTAATTTATTCACAACTAG+AGG - chr3_4:48912072-48912091 Msa0504450:intron 25.0%
! GAATGTTCATATTGAGAATA+TGG + chr3_4:48910751-48910770 None:intergenic 25.0%
!!! GAGAATGTTACTTTTATTAG+GGG + chr3_4:48910994-48911013 None:intergenic 25.0%
!!! CAGAGAATGTTACTTTTATT+AGG + chr3_4:48910996-48911015 None:intergenic 25.0%
! AATCTGTATGACACAATAAA+TGG - chr3_4:48911354-48911373 Msa0504450:intron 25.0%
! ATTGGAATTATTATCATGGA+TGG + chr3_4:48911427-48911446 None:intergenic 25.0%
! TCAATTCCTATCCAAATATT+GGG - chr3_4:48911874-48911893 Msa0504450:CDS 25.0%
! ACTAATTTATTCACAACTAG+AGG - chr3_4:48912072-48912091 Msa0504450:intron 25.0%
TTGAAATACGTCATGTCTTA+GGG + chr3_4:48910344-48910363 None:intergenic 30.0%
ATTGAAATACGTCATGTCTT+AGG + chr3_4:48910345-48910364 None:intergenic 30.0%
CTGAAGAACATTACTTACTT+AGG + chr3_4:48910384-48910403 None:intergenic 30.0%
TAATGTTCTTCAGTGTTCAT+TGG - chr3_4:48910391-48910410 Msa0504450:three_prime_UTR 30.0%
ATACAGGAACAACTTAATAC+CGG + chr3_4:48910417-48910436 None:intergenic 30.0%
! AATTATGGTTGATTGGTGAT+TGG - chr3_4:48910443-48910462 Msa0504450:three_prime_UTR 30.0%
!! CTTATTGTTGATTCTGTTCA+TGG - chr3_4:48910496-48910515 Msa0504450:three_prime_UTR 30.0%
TCATTCTCAGTAACTATGTT+TGG - chr3_4:48910562-48910581 Msa0504450:three_prime_UTR 30.0%
AAAAAGGCAATCAAAACAAG+AGG + chr3_4:48910653-48910672 None:intergenic 30.0%
ATTGAAAAGCTTCTTTACTG+TGG + chr3_4:48910785-48910804 None:intergenic 30.0%
ATCTCTCTTTGTTAGGAAAT+TGG + chr3_4:48910815-48910834 None:intergenic 30.0%
!! AATGTAAAAGTACTTCTCGA+AGG + chr3_4:48910851-48910870 None:intergenic 30.0%
TTCATTTGTAGTCTCTAGTT+TGG - chr3_4:48910967-48910986 Msa0504450:intron 30.0%
GAAAAATCATCTATTCAGTC+TGG - chr3_4:48911157-48911176 Msa0504450:intron 30.0%
TGGAATTATTATCATGGCTT+TGG + chr3_4:48911393-48911412 None:intergenic 30.0%
ATTATCATGGATGGTTATCA+TGG + chr3_4:48911463-48911482 None:intergenic 30.0%
GCAGTTAGTAAACTATATAC+AGG + chr3_4:48911548-48911567 None:intergenic 30.0%
!!! TTTTGAGATAGAGACAGAAA+TGG + chr3_4:48911591-48911610 None:intergenic 30.0%
CTCTATCTCAAAAGTTAGTT+TGG - chr3_4:48911598-48911617 Msa0504450:intron 30.0%
GAAGTATATTGAAATGCCAA+TGG - chr3_4:48911620-48911639 Msa0504450:intron 30.0%
!! TTGTACATATTTTGGACCAT+TGG + chr3_4:48911639-48911658 None:intergenic 30.0%
GGCTCAAATTAGAAAAAAGA+CGG + chr3_4:48911670-48911689 None:intergenic 30.0%
CTTAGATTATTCTTGACTTC+AGG + chr3_4:48911765-48911784 None:intergenic 30.0%
CTGAAGTCAAGAATAATCTA+AGG - chr3_4:48911763-48911782 Msa0504450:intron 30.0%
! AAGAATAATCTAAGGCCATT+TGG - chr3_4:48911771-48911790 Msa0504450:intron 30.0%
CTCAATTCCTATCCAAATAT+TGG - chr3_4:48911873-48911892 Msa0504450:CDS 30.0%
! ACTTTTCCCAATATTTGGAT+AGG + chr3_4:48911883-48911902 None:intergenic 30.0%
! GTCATACTTTTCCCAATATT+TGG + chr3_4:48911888-48911907 None:intergenic 30.0%
! ACCAGACAATTGATTAGTTA+TGG - chr3_4:48912213-48912232 Msa0504450:intron 30.0%
ACCATAACTAATCAATTGTC+TGG + chr3_4:48912217-48912236 None:intergenic 30.0%
TTGAAATACGTCATGTCTTA+GGG + chr3_4:48910344-48910363 None:intergenic 30.0%
ATTGAAATACGTCATGTCTT+AGG + chr3_4:48910345-48910364 None:intergenic 30.0%
CTGAAGAACATTACTTACTT+AGG + chr3_4:48910384-48910403 None:intergenic 30.0%
TAATGTTCTTCAGTGTTCAT+TGG - chr3_4:48910391-48910410 Msa0504450:three_prime_UTR 30.0%
ATACAGGAACAACTTAATAC+CGG + chr3_4:48910417-48910436 None:intergenic 30.0%
! AATTATGGTTGATTGGTGAT+TGG - chr3_4:48910443-48910462 Msa0504450:three_prime_UTR 30.0%
!! CTTATTGTTGATTCTGTTCA+TGG - chr3_4:48910496-48910515 Msa0504450:three_prime_UTR 30.0%
TCATTCTCAGTAACTATGTT+TGG - chr3_4:48910562-48910581 Msa0504450:three_prime_UTR 30.0%
AAAAAGGCAATCAAAACAAG+AGG + chr3_4:48910653-48910672 None:intergenic 30.0%
ATTGAAAAGCTTCTTTACTG+TGG + chr3_4:48910785-48910804 None:intergenic 30.0%
ATCTCTCTTTGTTAGGAAAT+TGG + chr3_4:48910815-48910834 None:intergenic 30.0%
!! AATGTAAAAGTACTTCTCGA+AGG + chr3_4:48910851-48910870 None:intergenic 30.0%
TTCATTTGTAGTCTCTAGTT+TGG - chr3_4:48910967-48910986 Msa0504450:intron 30.0%
GAAAAATCATCTATTCAGTC+TGG - chr3_4:48911157-48911176 Msa0504450:intron 30.0%
TGGAATTATTATCATGGCTT+TGG + chr3_4:48911393-48911412 None:intergenic 30.0%
ATTATCATGGATGGTTATCA+TGG + chr3_4:48911463-48911482 None:intergenic 30.0%
GCAGTTAGTAAACTATATAC+AGG + chr3_4:48911548-48911567 None:intergenic 30.0%
!!! TTTTGAGATAGAGACAGAAA+TGG + chr3_4:48911591-48911610 None:intergenic 30.0%
CTCTATCTCAAAAGTTAGTT+TGG - chr3_4:48911598-48911617 Msa0504450:intron 30.0%
GAAGTATATTGAAATGCCAA+TGG - chr3_4:48911620-48911639 Msa0504450:intron 30.0%
!! TTGTACATATTTTGGACCAT+TGG + chr3_4:48911639-48911658 None:intergenic 30.0%
GGCTCAAATTAGAAAAAAGA+CGG + chr3_4:48911670-48911689 None:intergenic 30.0%
CTTAGATTATTCTTGACTTC+AGG + chr3_4:48911765-48911784 None:intergenic 30.0%
CTGAAGTCAAGAATAATCTA+AGG - chr3_4:48911763-48911782 Msa0504450:intron 30.0%
! AAGAATAATCTAAGGCCATT+TGG - chr3_4:48911771-48911790 Msa0504450:intron 30.0%
CTCAATTCCTATCCAAATAT+TGG - chr3_4:48911873-48911892 Msa0504450:CDS 30.0%
! ACTTTTCCCAATATTTGGAT+AGG + chr3_4:48911883-48911902 None:intergenic 30.0%
! GTCATACTTTTCCCAATATT+TGG + chr3_4:48911888-48911907 None:intergenic 30.0%
! ACCAGACAATTGATTAGTTA+TGG - chr3_4:48912213-48912232 Msa0504450:intron 30.0%
ACCATAACTAATCAATTGTC+TGG + chr3_4:48912217-48912236 None:intergenic 30.0%
TGAAATACGTCATGTCTTAG+GGG + chr3_4:48910343-48910362 None:intergenic 35.0%
TCTCAGTAACTATGTTTGGA+TGG - chr3_4:48910566-48910585 Msa0504450:three_prime_UTR 35.0%
CTCAGTAACTATGTTTGGAT+GGG - chr3_4:48910567-48910586 Msa0504450:three_prime_UTR 35.0%
!!! TTATTTCTGTGCCGGTTTTA+TGG - chr3_4:48910627-48910646 Msa0504450:CDS 35.0%
AAAACAAGAGGCCATAAAAC+CGG + chr3_4:48910641-48910660 None:intergenic 35.0%
!!! TGCCTTTTTTGAGCATAAGT+TGG - chr3_4:48910664-48910683 Msa0504450:CDS 35.0%
AGCCAACTTATGCTCAAAAA+AGG + chr3_4:48910669-48910688 None:intergenic 35.0%
TCATATTGAGAATATGGCGT+TGG + chr3_4:48910745-48910764 None:intergenic 35.0%
! TCTTGTTCTTTTACTCTTGC+TGG - chr3_4:48911122-48911141 Msa0504450:intron 35.0%
! CTTGTTCTTTTACTCTTGCT+GGG - chr3_4:48911123-48911142 Msa0504450:intron 35.0%
TCTCGGCTGTTAATCTAATA+TGG - chr3_4:48911254-48911273 Msa0504450:intron 35.0%
! GACCTTAATTTGAGATCCAA+CGG - chr3_4:48911321-48911340 Msa0504450:intron 35.0%
AACCGTTGGATCTCAAATTA+AGG + chr3_4:48911326-48911345 None:intergenic 35.0%
!! CATGGCTTTGGATTTGAATT+TGG + chr3_4:48911381-48911400 None:intergenic 35.0%
AACTAGACTGGTGTCTATAT+TGG + chr3_4:48911490-48911509 None:intergenic 35.0%
! GAATCTTCTTTTGGTCCAAA+TGG + chr3_4:48911789-48911808 None:intergenic 35.0%
! GAGAGTCTTGAATCTTCTTT+TGG + chr3_4:48911798-48911817 None:intergenic 35.0%
AGTAAGATTCCAACTGCTTT+CGG + chr3_4:48912055-48912074 None:intergenic 35.0%
TTAAGAACAATCAACCACCA+CGG + chr3_4:48912277-48912296 None:intergenic 35.0%
TGAAATACGTCATGTCTTAG+GGG + chr3_4:48910343-48910362 None:intergenic 35.0%
TCTCAGTAACTATGTTTGGA+TGG - chr3_4:48910566-48910585 Msa0504450:three_prime_UTR 35.0%
CTCAGTAACTATGTTTGGAT+GGG - chr3_4:48910567-48910586 Msa0504450:three_prime_UTR 35.0%
!!! TTATTTCTGTGCCGGTTTTA+TGG - chr3_4:48910627-48910646 Msa0504450:CDS 35.0%
AAAACAAGAGGCCATAAAAC+CGG + chr3_4:48910641-48910660 None:intergenic 35.0%
!!! TGCCTTTTTTGAGCATAAGT+TGG - chr3_4:48910664-48910683 Msa0504450:CDS 35.0%
AGCCAACTTATGCTCAAAAA+AGG + chr3_4:48910669-48910688 None:intergenic 35.0%
TCATATTGAGAATATGGCGT+TGG + chr3_4:48910745-48910764 None:intergenic 35.0%
! TCTTGTTCTTTTACTCTTGC+TGG - chr3_4:48911122-48911141 Msa0504450:intron 35.0%
! CTTGTTCTTTTACTCTTGCT+GGG - chr3_4:48911123-48911142 Msa0504450:intron 35.0%
TCTCGGCTGTTAATCTAATA+TGG - chr3_4:48911254-48911273 Msa0504450:intron 35.0%
! GACCTTAATTTGAGATCCAA+CGG - chr3_4:48911321-48911340 Msa0504450:intron 35.0%
AACCGTTGGATCTCAAATTA+AGG + chr3_4:48911326-48911345 None:intergenic 35.0%
!! CATGGCTTTGGATTTGAATT+TGG + chr3_4:48911381-48911400 None:intergenic 35.0%
AACTAGACTGGTGTCTATAT+TGG + chr3_4:48911490-48911509 None:intergenic 35.0%
! GAATCTTCTTTTGGTCCAAA+TGG + chr3_4:48911789-48911808 None:intergenic 35.0%
! GAGAGTCTTGAATCTTCTTT+TGG + chr3_4:48911798-48911817 None:intergenic 35.0%
AGTAAGATTCCAACTGCTTT+CGG + chr3_4:48912055-48912074 None:intergenic 35.0%
TTAAGAACAATCAACCACCA+CGG + chr3_4:48912277-48912296 None:intergenic 35.0%
GAAATACGTCATGTCTTAGG+GGG + chr3_4:48910342-48910361 None:intergenic 40.0%
!! TACTTACTTAGGTTGAGCAC+TGG + chr3_4:48910373-48910392 None:intergenic 40.0%
TCTTGCAGTGATTCATGAAC+CGG - chr3_4:48910714-48910733 Msa0504450:CDS 40.0%
AAAAGCTTCTTTACTGTGGC+AGG + chr3_4:48910781-48910800 None:intergenic 40.0%
TCCTAACAAAGAGAGATGCA+TGG - chr3_4:48910818-48910837 Msa0504450:CDS 40.0%
CCTAACAAAGAGAGATGCAT+GGG - chr3_4:48910819-48910838 Msa0504450:CDS 40.0%
AGAAGAAGTAGATGCAAACC+TGG + chr3_4:48910898-48910917 None:intergenic 40.0%
! TTGCATGCAGTTTTCGATCT+CGG - chr3_4:48911237-48911256 Msa0504450:intron 40.0%
CATGGCTTCTTAAACTAGAC+TGG + chr3_4:48911502-48911521 None:intergenic 40.0%
GTGAAGAGATTTCTTGAAGC+AGG - chr3_4:48911942-48911961 Msa0504450:intron 40.0%
GAGATTTCTTGAAGCAGGAA+AGG - chr3_4:48911947-48911966 Msa0504450:intron 40.0%
ACTCTGCTATTGTCACAATG+AGG - chr3_4:48911994-48912013 Msa0504450:intron 40.0%
AACAGAAACTCTGCTGGTAA+GGG + chr3_4:48912024-48912043 None:intergenic 40.0%
GTAAGATTCCAACTGCTTTC+GGG + chr3_4:48912054-48912073 None:intergenic 40.0%
!! GCTTTGTTTGAGAAGATGGA+TGG + chr3_4:48912386-48912405 None:intergenic 40.0%
GAAATACGTCATGTCTTAGG+GGG + chr3_4:48910342-48910361 None:intergenic 40.0%
!! TACTTACTTAGGTTGAGCAC+TGG + chr3_4:48910373-48910392 None:intergenic 40.0%
TCTTGCAGTGATTCATGAAC+CGG - chr3_4:48910714-48910733 Msa0504450:CDS 40.0%
AAAAGCTTCTTTACTGTGGC+AGG + chr3_4:48910781-48910800 None:intergenic 40.0%
TCCTAACAAAGAGAGATGCA+TGG - chr3_4:48910818-48910837 Msa0504450:CDS 40.0%
CCTAACAAAGAGAGATGCAT+GGG - chr3_4:48910819-48910838 Msa0504450:CDS 40.0%
AGAAGAAGTAGATGCAAACC+TGG + chr3_4:48910898-48910917 None:intergenic 40.0%
! TTGCATGCAGTTTTCGATCT+CGG - chr3_4:48911237-48911256 Msa0504450:intron 40.0%
CATGGCTTCTTAAACTAGAC+TGG + chr3_4:48911502-48911521 None:intergenic 40.0%
GTGAAGAGATTTCTTGAAGC+AGG - chr3_4:48911942-48911961 Msa0504450:intron 40.0%
GAGATTTCTTGAAGCAGGAA+AGG - chr3_4:48911947-48911966 Msa0504450:intron 40.0%
ACTCTGCTATTGTCACAATG+AGG - chr3_4:48911994-48912013 Msa0504450:intron 40.0%
AACAGAAACTCTGCTGGTAA+GGG + chr3_4:48912024-48912043 None:intergenic 40.0%
GTAAGATTCCAACTGCTTTC+GGG + chr3_4:48912054-48912073 None:intergenic 40.0%
!! GCTTTGTTTGAGAAGATGGA+TGG + chr3_4:48912386-48912405 None:intergenic 40.0%
GTTCTTCAGTGTTCATTGGC+CGG - chr3_4:48910395-48910414 Msa0504450:three_prime_UTR 45.0%
GGTTAGTAAGGCACAGCTTA+CGG + chr3_4:48910593-48910612 None:intergenic 45.0%
CCCATGCATCTCTCTTTGTT+AGG + chr3_4:48910822-48910841 None:intergenic 45.0%
AAACCTGGACAATCATCACG+AGG + chr3_4:48910883-48910902 None:intergenic 45.0%
ACCAATAGACTGAAGCTACC+AGG - chr3_4:48911182-48911201 Msa0504450:intron 45.0%
TCCTGGTAGCTTCAGTCTAT+TGG + chr3_4:48911186-48911205 None:intergenic 45.0%
ACTGCAATGAATCCAGATCC+TGG + chr3_4:48911203-48911222 None:intergenic 45.0%
AGGATCTGGATTCATTGCAG+TGG - chr3_4:48911202-48911221 Msa0504450:intron 45.0%
CAGATTTCGATCTCAACCGT+TGG + chr3_4:48911340-48911359 None:intergenic 45.0%
CAACAGAAACTCTGCTGGTA+AGG + chr3_4:48912025-48912044 None:intergenic 45.0%
CTTACCAGCAGAGTTTCTGT+TGG - chr3_4:48912023-48912042 Msa0504450:intron 45.0%
GGACTTGATTAATCACACCG+TGG - chr3_4:48912257-48912276 Msa0504450:intron 45.0%
CTTGATTAATCACACCGTGG+TGG - chr3_4:48912260-48912279 Msa0504450:intron 45.0%
GTTCTTCAGTGTTCATTGGC+CGG - chr3_4:48910395-48910414 Msa0504450:three_prime_UTR 45.0%
GGTTAGTAAGGCACAGCTTA+CGG + chr3_4:48910593-48910612 None:intergenic 45.0%
CCCATGCATCTCTCTTTGTT+AGG + chr3_4:48910822-48910841 None:intergenic 45.0%
AAACCTGGACAATCATCACG+AGG + chr3_4:48910883-48910902 None:intergenic 45.0%
ACCAATAGACTGAAGCTACC+AGG - chr3_4:48911182-48911201 Msa0504450:intron 45.0%
TCCTGGTAGCTTCAGTCTAT+TGG + chr3_4:48911186-48911205 None:intergenic 45.0%
ACTGCAATGAATCCAGATCC+TGG + chr3_4:48911203-48911222 None:intergenic 45.0%
AGGATCTGGATTCATTGCAG+TGG - chr3_4:48911202-48911221 Msa0504450:intron 45.0%
CAGATTTCGATCTCAACCGT+TGG + chr3_4:48911340-48911359 None:intergenic 45.0%
CAACAGAAACTCTGCTGGTA+AGG + chr3_4:48912025-48912044 None:intergenic 45.0%
CTTACCAGCAGAGTTTCTGT+TGG - chr3_4:48912023-48912042 Msa0504450:intron 45.0%
GGACTTGATTAATCACACCG+TGG - chr3_4:48912257-48912276 Msa0504450:intron 45.0%
CTTGATTAATCACACCGTGG+TGG - chr3_4:48912260-48912279 Msa0504450:intron 45.0%
!!! GAAATAATAAAAAAAAATTT+TGG + chr3_4:48910614-48910633 None:intergenic 5.0%
!!! GAAATAATAAAAAAAAATTT+TGG + chr3_4:48910614-48910633 None:intergenic 5.0%
ATACGTCATGTCTTAGGGGG+AGG + chr3_4:48910339-48910358 None:intergenic 50.0%
GCAGTGATTCATGAACCGGA+TGG - chr3_4:48910718-48910737 Msa0504450:CDS 50.0%
! GAATATGGCGTTGGACCATC+CGG + chr3_4:48910736-48910755 None:intergenic 50.0%
ACAAAGAGAGATGCATGGGC+TGG - chr3_4:48910823-48910842 Msa0504450:CDS 50.0%
ACTCCTCGTGATGATTGTCC+AGG - chr3_4:48910877-48910896 Msa0504450:CDS 50.0%
AGACTGAAGCTACCAGGATC+TGG - chr3_4:48911188-48911207 Msa0504450:intron 50.0%
TCTATGACTGCACTCACGCA+TGG + chr3_4:48911520-48911539 None:intergenic 50.0%
CAGCTAGCATCACAGCTCAA+CGG - chr3_4:48911840-48911859 Msa0504450:CDS 50.0%
GGAAAGGCTGCTCTTAAAGC+TGG - chr3_4:48911963-48911982 Msa0504450:intron 50.0%
GAAACTCTGCTGGTAAGGGA+AGG + chr3_4:48912020-48912039 None:intergenic 50.0%
ACCAGCAGAGTTTCTGTTGG+AGG - chr3_4:48912026-48912045 Msa0504450:intron 50.0%
ACCTCCAACAGAAACTCTGC+TGG + chr3_4:48912030-48912049 None:intergenic 50.0%
ATACGTCATGTCTTAGGGGG+AGG + chr3_4:48910339-48910358 None:intergenic 50.0%
GCAGTGATTCATGAACCGGA+TGG - chr3_4:48910718-48910737 Msa0504450:CDS 50.0%
! GAATATGGCGTTGGACCATC+CGG + chr3_4:48910736-48910755 None:intergenic 50.0%
ACAAAGAGAGATGCATGGGC+TGG - chr3_4:48910823-48910842 Msa0504450:CDS 50.0%
ACTCCTCGTGATGATTGTCC+AGG - chr3_4:48910877-48910896 Msa0504450:CDS 50.0%
AGACTGAAGCTACCAGGATC+TGG - chr3_4:48911188-48911207 Msa0504450:intron 50.0%
TCTATGACTGCACTCACGCA+TGG + chr3_4:48911520-48911539 None:intergenic 50.0%
CAGCTAGCATCACAGCTCAA+CGG - chr3_4:48911840-48911859 Msa0504450:CDS 50.0%
GGAAAGGCTGCTCTTAAAGC+TGG - chr3_4:48911963-48911982 Msa0504450:intron 50.0%
GAAACTCTGCTGGTAAGGGA+AGG + chr3_4:48912020-48912039 None:intergenic 50.0%
ACCAGCAGAGTTTCTGTTGG+AGG - chr3_4:48912026-48912045 Msa0504450:intron 50.0%
ACCTCCAACAGAAACTCTGC+TGG + chr3_4:48912030-48912049 None:intergenic 50.0%
CGTTGAGCTGTGATGCTAGC+TGG + chr3_4:48911842-48911861 None:intergenic 55.0%
TGGAGGTTCCCGAAAGCAGT+TGG - chr3_4:48912043-48912062 Msa0504450:intron 55.0%
CGTTGAGCTGTGATGCTAGC+TGG + chr3_4:48911842-48911861 None:intergenic 55.0%
TGGAGGTTCCCGAAAGCAGT+TGG - chr3_4:48912043-48912062 Msa0504450:intron 55.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 48910267 48912407 48910267 ID=Msa0504450;Name=Msa0504450
chr3_4 mRNA 48910267 48912407 48910267 ID=Msa0504450-mRNA-1;Parent=Msa0504450;Name=Msa0504450-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0|0.5|0.33|0.66|1|1|3|331|172
chr3_4 exon 48910267 48910920 48910267 ID=Msa0504450-mRNA-1:exon:10530;Parent=Msa0504450-mRNA-1
chr3_4 exon 48911776 48911952 48911776 ID=Msa0504450-mRNA-1:exon:10529;Parent=Msa0504450-mRNA-1
chr3_4 exon 48912389 48912407 48912389 ID=Msa0504450-mRNA-1:exon:10528;Parent=Msa0504450-mRNA-1
chr3_4 CDS 48912389 48912407 48912389 ID=Msa0504450-mRNA-1:cds;Parent=Msa0504450-mRNA-1
chr3_4 CDS 48911776 48911952 48911776 ID=Msa0504450-mRNA-1:cds;Parent=Msa0504450-mRNA-1
chr3_4 CDS 48910598 48910920 48910598 ID=Msa0504450-mRNA-1:cds;Parent=Msa0504450-mRNA-1
chr3_4 three_prime_UTR 48910267 48910597 48910267 ID=Msa0504450-mRNA-1:three_prime_utr;Parent=Msa0504450-mRNA-1
Gene Sequence

>Msa0504450

ATGAAAATTAGGAATTTAGTGATTCATGAACCGGATGGTCCAACGCCATATTCTCAATATGAACATTCATCTATTCCTGCCACAGTAAAGAAGCTTTTCAATTTAAAATCCAATTTCCTAACAAAGAGAGATGCATGGGCTGGTACCTTCGAGAAGTACTTTTACATTCGAGATACTCCTCGTGATGATTGTCCAGAAACACTTCCTGAAGTCAAGAATAATCTAAGGCCATTTGGACCAAAAGAAGATTCAAGACTCTCAGAATTTCAAATAGAACTGATCCAGCTAGCATCACAGCTCAACGGCGATTACGTACTCAATTCCTATCCAAATATTGGGAAAAGTATGACAGTGAAAGAAGCAAACAGATATGCAGAAGATGCAGTGAAGAGATTTCTTGAAGCAGGAAAGGCTGCTCTTAAAGCTGGTGCTAATGACTCTGCTATTGTCACAATGAGGCCTTCCCTTACCAGCAGAGTTTCTGTTGGAGGTTCCCGAAAGCAGTTGGAATCTTACTAA
Protein sequence

>Msa0504450

MKIRNLVIHEPDGPTPYSQYEHSSIPATVKKLFNLKSNFLTKRDAWAGTFEKYFYIRDTPRDDCPETLPEVKNNLRPFGPKEDSRLSEFQIELIQLASQLNGDYVLNSYPNIGKSMTVKEANRYAEDAVKRFLEAGKAALKAGANDSAIVTMRPSLTSRVSVGGSRKQLESY*