Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1419970 | sp|Q94JU2|WTR18_ARATH | 45.427 | 328 | 168 | 4 | 10 | 333 | 14 | 334 | 9.13e-89 | 274 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0435010 | Msa1419970 | 0.800932 | 1.142681e-48 | -8.615850e-47 |
| Msa0468130 | Msa1419970 | 0.801207 | 1.004154e-48 | -8.615850e-47 |
| Msa0504450 | Msa1419970 | 0.815791 | 7.714261e-52 | -8.615850e-47 |
| Msa0722910 | Msa1419970 | 0.837700 | 4.453674e-57 | -8.615850e-47 |
| Msa0722920 | Msa1419970 | 0.953469 | 2.559944e-111 | -8.615850e-47 |
| Msa0723110 | Msa1419970 | 0.813748 | 2.187494e-51 | -8.615850e-47 |
| Msa0723120 | Msa1419970 | 0.801143 | 1.034866e-48 | -8.615850e-47 |
| Msa0723130 | Msa1419970 | 0.918631 | 1.227675e-86 | -8.615850e-47 |
| Msa0843250 | Msa1419970 | 0.806883 | 6.626056e-50 | -8.615850e-47 |
| Msa0870150 | Msa1419970 | 0.847222 | 1.325282e-59 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1419970 | MtrunA17_Chr5g0413951 | 94.000 | 350 | 21 | 0 | 1 | 350 | 1 | 350 | 0.0 | 635 |
| Msa1419970 | MtrunA17_Chr5g0413941 | 85.143 | 350 | 52 | 0 | 1 | 350 | 1 | 350 | 0.0 | 593 |
| Msa1419970 | MtrunA17_Chr5g0413931 | 73.684 | 342 | 90 | 0 | 8 | 349 | 7 | 348 | 0.0 | 522 |
| Msa1419970 | MtrunA17_Chr5g0413921 | 69.653 | 346 | 100 | 1 | 1 | 346 | 1 | 341 | 5.63e-176 | 492 |
| Msa1419970 | MtrunA17_Chr7g0227921 | 44.152 | 342 | 184 | 3 | 12 | 349 | 16 | 354 | 4.55e-86 | 264 |
| Msa1419970 | MtrunA17_Chr3g0126101 | 42.353 | 340 | 190 | 5 | 12 | 350 | 11 | 345 | 6.24e-83 | 255 |
| Msa1419970 | MtrunA17_Chr4g0059311 | 42.012 | 338 | 191 | 4 | 12 | 345 | 16 | 352 | 1.14e-80 | 249 |
| Msa1419970 | MtrunA17_Chr5g0413961 | 39.763 | 337 | 197 | 3 | 12 | 346 | 19 | 351 | 2.72e-77 | 241 |
| Msa1419970 | MtrunA17_Chr6g0451251 | 45.614 | 342 | 176 | 5 | 12 | 347 | 17 | 354 | 3.34e-76 | 239 |
| Msa1419970 | MtrunA17_Chr1g0150151 | 38.824 | 340 | 199 | 5 | 10 | 345 | 14 | 348 | 1.08e-75 | 237 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1419970 | AT3G28050.1 | 45.427 | 328 | 168 | 4 | 10 | 333 | 14 | 334 | 9.28e-90 | 274 |
| Msa1419970 | AT5G40230.1 | 42.566 | 343 | 191 | 2 | 10 | 349 | 23 | 362 | 1.31e-89 | 273 |
| Msa1419970 | AT5G40240.1 | 41.003 | 339 | 186 | 3 | 12 | 343 | 24 | 355 | 2.24e-87 | 268 |
| Msa1419970 | AT5G40240.3 | 41.003 | 339 | 186 | 3 | 12 | 343 | 24 | 355 | 2.24e-87 | 268 |
| Msa1419970 | AT5G40240.2 | 41.003 | 339 | 186 | 3 | 12 | 343 | 38 | 369 | 5.88e-87 | 267 |
| Msa1419970 | AT5G40210.1 | 41.642 | 341 | 179 | 5 | 8 | 345 | 13 | 336 | 1.06e-77 | 242 |
| Msa1419970 | AT4G15540.1 | 42.647 | 340 | 182 | 2 | 10 | 349 | 19 | 345 | 4.85e-75 | 235 |
| Msa1419970 | AT4G15540.2 | 42.647 | 340 | 182 | 2 | 10 | 349 | 19 | 345 | 4.85e-75 | 235 |
| Msa1419970 | AT3G28080.1 | 41.787 | 347 | 189 | 6 | 10 | 349 | 16 | 356 | 1.48e-69 | 222 |
| Msa1419970 | AT3G28100.1 | 42.202 | 327 | 173 | 4 | 10 | 327 | 16 | 335 | 1.18e-68 | 219 |
Find 63 sgRNAs with CRISPR-Local
Find 377 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATTGATGTTGAAATTGG+AGG | 0.473060 | tig0024733:-66533 | Msa1419970:intergenic |
| AAACAAAAGCATGGATATAT+AGG | 0.382758 | tig0024733:+71002 | Msa1419970:CDS |
| AACAATAGCTGAAGCCAACG+TGG | 0.634527 | tig0024733:-66751 | Msa1419970:intergenic |
| AATAATTTGTTACGTCGGAT+CGG | 0.538176 | tig0024733:-66211 | Msa1419970:intergenic |
| AATATAGAAATGGGAGCAAG+TGG | 0.531385 | tig0024733:+69740 | Msa1419970:CDS |
| ACAATAGCTGAAGCCAACGT+GGG | 0.701649 | tig0024733:-66750 | Msa1419970:intergenic |
| ACAGATAGCAACTGTTTATC+AGG | 0.412768 | tig0024733:-70347 | Msa1419970:intergenic |
| ACATGGGCATGTCATAAGAA+AGG | 0.572940 | tig0024733:+70655 | Msa1419970:CDS |
| ACTCATATGTTAATGACTTC+TGG | 0.288657 | tig0024733:+66711 | Msa1419970:CDS |
| ACTTATTGGTGCAGCTTGAT+TGG | 0.350269 | tig0024733:+70868 | Msa1419970:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTGAGAATTAACAAGCAAAT+TGG | + | tig0024733:66291-66310 | Msa1419970:five_prime_UTR | 30.0% | |
| GAAGAAAACATAGAGCTAAA+AGG | - | tig0024733:66475-66494 | Msa1419970:intergenic | 30.0% | |
| TTTGGCAAAATAGAGTGATA+TGG | + | tig0024733:66665-66684 | Msa1419970:intron | 30.0% | |
| ACTCATATGTTAATGACTTC+TGG | + | tig0024733:66711-66730 | Msa1419970:CDS | 30.0% | |
| TAGAAGTCAACTAACTAAGA+TGG | - | tig0024733:67151-67170 | Msa1419970:intergenic | 30.0% | |
| AGTGGATCATATGTATAAGA+TGG | + | tig0024733:67314-67333 | Msa1419970:intron | 30.0% | |
| TAATCGACTGAAACAATGTT+TGG | + | tig0024733:67353-67372 | Msa1419970:intron | 30.0% | |
| TTTGGTTAAATGTGTGTTAG+AGG | + | tig0024733:67371-67390 | Msa1419970:intron | 30.0% | |
| TGAATAAATATGGGGATAAC+GGG | - | tig0024733:67701-67720 | Msa1419970:intergenic | 30.0% | |
| CCCATATTTATTCATATTGG+TGG | + | tig0024733:67707-67726 | Msa1419970:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0024733 | gene | 66209 | 71336 | 66209 | ID=Msa1419970;Name=Msa1419970 |
| tig0024733 | mRNA | 66209 | 71336 | 66209 | ID=Msa1419970-mRNA-1;Parent=Msa1419970;Name=Msa1419970-mRNA-1;_AED=0.25;_eAED=0.25;_QI=132|1|1|1|1|1|6|313|350 |
| tig0024733 | exon | 66209 | 66585 | 66209 | ID=Msa1419970-mRNA-1:exon:1187;Parent=Msa1419970-mRNA-1 |
| tig0024733 | exon | 66704 | 66817 | 66704 | ID=Msa1419970-mRNA-1:exon:1188;Parent=Msa1419970-mRNA-1 |
| tig0024733 | exon | 69613 | 69853 | 69613 | ID=Msa1419970-mRNA-1:exon:1189;Parent=Msa1419970-mRNA-1 |
| tig0024733 | exon | 70221 | 70379 | 70221 | ID=Msa1419970-mRNA-1:exon:1190;Parent=Msa1419970-mRNA-1 |
| tig0024733 | exon | 70619 | 70770 | 70619 | ID=Msa1419970-mRNA-1:exon:1191;Parent=Msa1419970-mRNA-1 |
| tig0024733 | exon | 70882 | 71336 | 70882 | ID=Msa1419970-mRNA-1:exon:1192;Parent=Msa1419970-mRNA-1 |
| tig0024733 | five_prime_UTR | 66209 | 66340 | 66209 | ID=Msa1419970-mRNA-1:five_prime_utr;Parent=Msa1419970-mRNA-1 |
| tig0024733 | CDS | 66341 | 66585 | 66341 | ID=Msa1419970-mRNA-1:cds;Parent=Msa1419970-mRNA-1 |
| tig0024733 | CDS | 66704 | 66817 | 66704 | ID=Msa1419970-mRNA-1:cds;Parent=Msa1419970-mRNA-1 |
| tig0024733 | CDS | 69613 | 69853 | 69613 | ID=Msa1419970-mRNA-1:cds;Parent=Msa1419970-mRNA-1 |
| tig0024733 | CDS | 70221 | 70379 | 70221 | ID=Msa1419970-mRNA-1:cds;Parent=Msa1419970-mRNA-1 |
| tig0024733 | CDS | 70619 | 70770 | 70619 | ID=Msa1419970-mRNA-1:cds;Parent=Msa1419970-mRNA-1 |
| tig0024733 | CDS | 70882 | 71023 | 70882 | ID=Msa1419970-mRNA-1:cds;Parent=Msa1419970-mRNA-1 |
| tig0024733 | three_prime_UTR | 71024 | 71336 | 71024 | ID=Msa1419970-mRNA-1:three_prime_utr;Parent=Msa1419970-mRNA-1 |
| Gene Sequence |
>Msa1419970 ATGGCGAGTGGAGGAGCAAGTGTAACAACAACAATGATTGCAGCTCAAATCGTTGAAGTGGGTGGTGAAACTCTAATGAAATCAGCCACAAAAGATGGAATGAGTGTTTTTATTTTCATTGTTTACACAAACCTTTTAGCTCTATGTTTTCTTCTACCATCCACTCTCTTCCACCATAGAAAAAGAGCTCCTCCTCCAATTTCAACATCAATTTTCTATAGATTACTTCTTCTTGGTTGCCTCAAAACTGCAACTCATATGTTAATGACTTCTGGAATTAAATTCAGTTCTCCCACGTTGGCTTCAGCTATTGTTAATCTTGTCCCTGCTTTTACCTTCATACTTGCTATAATTTCAAGGATGGAAGTTCTAAATATGAAAAAGCATAGCAGCCAGGCAAAAGTTATTGGCACAGTGGTCTCTATTGCAGGGGCATTAGTTGTGACATTTTATAAAGGGATACCATTGATTAATGATGCCAATAAAAATATAGAAATGGGAGCAAGTGGAATTTTCCTTTTAGGGAAATCAGATTGGATAATTGGTGCTTTTCTTCTTGCAGCGGGTAGCTTTTGTCTTTCTCTTCTGTTTATTGTCCAGACTTGGATTGTCAGGGATTATCCAGAAGAGCTAGTGATAAACACCATTTGTTGCTGTTTTGTGGTAATCATATCTACCATTGTAGCTCTCATTGCAGAGGGAAATTCAAATGCTTGGAGACTCAGACCTGATAAACAGTTGCTATCTGTTGTTTACTCAGCAGCTTTTGTGGAATCATTGAAGAATGTTATACACACATGGGCATGTCATAAGAAAGGACCAATATATGTAGCTATGTTCAAACCTTTAAGAGTGGTCATTGCACTTAGCATGGGGGTTATATTTTTGGGAGACAATCTTTATCTTGGAAGCTTGATTGGAGCTGCTATCATAGTTATTGGATTTTATGCTGTGATATGGGCACAAGCTCAAGAGGAACACATAACAAGTGAAAATAACATTTTGTCATCTTCTTCAGCCCCTCTGTTGTCAAACAAAAGCATGGATATATAG |
| Protein sequence |
>Msa1419970 MASGGASVTTTMIAAQIVEVGGETLMKSATKDGMSVFIFIVYTNLLALCFLLPSTLFHHRKRAPPPISTSIFYRLLLLGCLKTATHMLMTSGIKFSSPTLASAIVNLVPAFTFILAIISRMEVLNMKKHSSQAKVIGTVVSIAGALVVTFYKGIPLINDANKNIEMGASGIFLLGKSDWIIGAFLLAAGSFCLSLLFIVQTWIVRDYPEELVINTICCCFVVIISTIVALIAEGNSNAWRLRPDKQLLSVVYSAAFVESLKNVIHTWACHKKGPIYVAMFKPLRVVIALSMGVIFLGDNLYLGSLIGAAIIVIGFYAVIWAQAQEEHITSENNILSSSSAPLLSNKSMDI* |
