AlfalfaGEDB | Msa1234690

M. sativa cultivar zhongmu-4 / Msa1234690

KEGG: K09512
GO: GO:0003674 GO:0005488 GO:0005515 GO:0051087
PFAM: PF00226.34

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1234690	XP_003627374.1	97.468	237	4	2	1	237	1	235	1.05e-165	467

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1234690	sp\|Q72GN6\|DNAJ_THET2	33.333	120	74	1	9	128	2	115	7.52e-16	79.0

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006040	Msa1234690	0.880953	3.588662e-70	-8.615850e-47
Msa0032610	Msa1234690	0.827661	1.383280e-54	-8.615850e-47
Msa0036160	Msa1234690	0.827007	1.984627e-54	-8.615850e-47
Msa0042980	Msa1234690	-0.808258	3.383151e-50	-8.615850e-47
Msa0054750	Msa1234690	0.867790	1.055571e-65	-8.615850e-47
Msa0111310	Msa1234690	0.810241	1.271130e-50	-8.615850e-47
Msa0615270	Msa1234690	-0.800555	1.364258e-48	-8.615850e-47
Msa0629650	Msa1234690	0.811743	6.009336e-51	-8.615850e-47
Msa0650730	Msa1234690	0.804628	1.971407e-49	-8.615850e-47
Msa0654010	Msa1234690	0.814287	1.663300e-51	-8.615850e-47
Msa0661210	Msa1234690	0.872363	3.368594e-67	-8.615850e-47
Msa0671740	Msa1234690	0.801878	7.313219e-49	-8.615850e-47
Msa0684850	Msa1234690	0.825735	3.985329e-54	-8.615850e-47
Msa0723130	Msa1234690	0.806100	9.691723e-50	-8.615850e-47
Msa0755080	Msa1234690	0.845242	4.588435e-59	-8.615850e-47
Msa0782470	Msa1234690	0.811807	5.820155e-51	-8.615850e-47
Msa0795920	Msa1234690	0.850188	1.995548e-60	-8.615850e-47
Msa0796950	Msa1234690	0.832980	6.934483e-56	-8.615850e-47
Msa0797970	Msa1234690	0.857427	1.644930e-62	-8.615850e-47
Msa0801360	Msa1234690	0.809167	2.162963e-50	-8.615850e-47
Msa0834450	Msa1234690	0.845368	4.242304e-59	-8.615850e-47
Msa0839830	Msa1234690	0.807293	5.424463e-50	-8.615850e-47
Msa0148120	Msa1234690	0.840144	1.037773e-57	-8.615850e-47
Msa0172780	Msa1234690	0.804237	2.378437e-49	-8.615850e-47
Msa0178860	Msa1234690	-0.800081	1.703349e-48	-8.615850e-47
Msa0197180	Msa1234690	0.826336	2.868874e-54	-8.615850e-47
Msa0223380	Msa1234690	0.803666	3.126518e-49	-8.615850e-47
Msa1234690	Msa1234730	0.977508	6.394284e-144	-8.615850e-47
Msa1234690	Msa1260130	0.802958	4.381751e-49	-8.615850e-47
Msa1234690	Msa1282920	0.816050	6.751122e-52	-8.615850e-47
Msa1234690	Msa1301650	0.825771	3.907494e-54	-8.615850e-47
Msa1234690	Msa1302100	0.815654	8.275027e-52	-8.615850e-47
Msa1234690	Msa1307740	0.829218	5.822165e-55	-8.615850e-47
Msa1234690	Msa1315110	0.977061	4.921096e-143	-8.615850e-47
Msa1234690	Msa1322190	0.809417	1.911465e-50	-8.615850e-47
Msa1234690	Msa1342100	0.805163	1.524303e-49	-8.615850e-47
Msa1234690	Msa1359630	0.870770	1.135321e-66	-8.615850e-47
Msa1234690	Msa1368980	0.804684	1.919811e-49	-8.615850e-47
Msa1234690	Msa1403340	0.814115	1.815956e-51	-8.615850e-47
Msa1234690	Msa1419970	0.820042	8.452634e-53	-8.615850e-47
Msa1234690	Msa1427830	0.808523	2.970165e-50	-8.615850e-47
Msa1234690	Msa1459760	-0.806715	7.189241e-50	-8.615850e-47
Msa1234690	Msa1465960	0.814125	1.806243e-51	-8.615850e-47
Msa0379410	Msa1234690	0.826459	2.681323e-54	-8.615850e-47
Msa0380040	Msa1234690	0.810432	1.156111e-50	-8.615850e-47
Msa0389810	Msa1234690	0.830781	2.420528e-55	-8.615850e-47
Msa0394340	Msa1234690	0.825803	3.840842e-54	-8.615850e-47
Msa0429290	Msa1234690	0.808301	3.312783e-50	-8.615850e-47
Msa0442200	Msa1234690	0.814577	1.435303e-51	-8.615850e-47
Msa0457510	Msa1234690	0.818139	2.290900e-52	-8.615850e-47
Msa0468130	Msa1234690	0.833569	4.945889e-56	-8.615850e-47
Msa0475240	Msa1234690	0.811465	6.907821e-51	-8.615850e-47
Msa0475580	Msa1234690	0.822385	2.437338e-53	-8.615850e-47
Msa0982270	Msa1234690	0.832844	7.497304e-56	-8.615850e-47
Msa1012830	Msa1234690	0.803664	3.128770e-49	-8.615850e-47
Msa1012920	Msa1234690	0.806376	8.475670e-50	-8.615850e-47
Msa1021340	Msa1234690	0.811104	8.274136e-51	-8.615850e-47
Msa1037020	Msa1234690	0.800904	1.157996e-48	-8.615850e-47
Msa1054410	Msa1234690	0.843376	1.456007e-58	-8.615850e-47
Msa1077670	Msa1234690	0.845493	3.924669e-59	-8.615850e-47
Msa1079730	Msa1234690	-0.839013	2.042435e-57	-8.615850e-47
Msa1089040	Msa1234690	0.804415	2.183803e-49	-8.615850e-47
Msa0265380	Msa1234690	0.830352	3.081867e-55	-8.615850e-47
Msa0267310	Msa1234690	0.808656	2.782560e-50	-8.615850e-47
Msa0268090	Msa1234690	0.838460	2.838820e-57	-8.615850e-47
Msa0303360	Msa1234690	0.838674	2.499520e-57	-8.615850e-47
Msa0308770	Msa1234690	0.836068	1.162050e-56	-8.615850e-47
Msa0347610	Msa1234690	0.829166	5.993270e-55	-8.615850e-47
Msa1124540	Msa1234690	-0.806880	6.634627e-50	-8.615850e-47
Msa1132890	Msa1234690	0.820561	6.428432e-53	-8.615850e-47
Msa1148750	Msa1234690	0.808664	2.771679e-50	-8.615850e-47
Msa1162980	Msa1234690	0.827366	1.628069e-54	-8.615850e-47
Msa1164530	Msa1234690	0.802158	6.405588e-49	-8.615850e-47
Msa1168040	Msa1234690	-0.839545	1.485908e-57	-8.615850e-47
Msa1174870	Msa1234690	0.981930	8.396746e-154	-8.615850e-47
Msa1176070	Msa1234690	0.805277	1.443027e-49	-8.615850e-47
Msa1187520	Msa1234690	0.804946	1.691800e-49	-8.615850e-47
Msa1219810	Msa1234690	0.843468	1.375986e-58	-8.615850e-47
Msa0489610	Msa1234690	0.822951	1.799094e-53	-8.615850e-47
Msa0504410	Msa1234690	0.856622	2.841051e-62	-8.615850e-47
Msa0504450	Msa1234690	0.802558	5.299225e-49	-8.615850e-47
Msa0516160	Msa1234690	0.806127	9.565451e-50	-8.615850e-47
Msa0519220	Msa1234690	0.818228	2.186622e-52	-8.615850e-47
Msa0532540	Msa1234690	0.810084	1.373951e-50	-8.615850e-47
Msa0561130	Msa1234690	-0.808268	3.366462e-50	-8.615850e-47
Msa0580420	Msa1234690	0.830867	2.306123e-55	-8.615850e-47
Msa0589390	Msa1234690	0.801592	8.374687e-49	-8.615850e-47
Msa0592720	Msa1234690	0.801535	8.600855e-49	-8.615850e-47
Msa0859380	Msa1234690	0.818964	1.489074e-52	-8.615850e-47
Msa0870150	Msa1234690	0.846760	1.774023e-59	-8.615850e-47
Msa0901630	Msa1234690	0.806734	7.124872e-50	-8.615850e-47
Msa0903400	Msa1234690	0.810078	1.378250e-50	-8.615850e-47
Msa0935850	Msa1234690	0.840326	9.299871e-58	-8.615850e-47
Msa0941540	Msa1234690	0.806891	6.598989e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1234690	MtrunA17_Chr8g0344721	83.260	227	29	3	1	219	1	226	1.67e-132	372
Msa1234690	MtrunA17_Chr7g0271891	45.161	124	62	3	11	134	17	134	1.13e-31	114
Msa1234690	MtrunA17_Chr4g0044331	43.307	127	66	3	16	139	18	141	1.20e-28	105
Msa1234690	MtrunA17_Chr7g0263971	49.333	75	33	1	6	80	365	434	2.60e-15	74.7
Msa1234690	MtrunA17_Chr2g0282761	47.826	69	31	1	10	78	4	67	3.34e-15	73.6
Msa1234690	MtrunA17_Chr4g0021141	45.455	77	36	1	3	79	15	85	5.37e-15	69.7
Msa1234690	MtrunA17_Chr2g0324921	49.275	69	30	1	10	78	4	67	5.42e-15	73.2
Msa1234690	MtrunA17_Chr2g0295831	47.561	82	36	2	9	90	109	183	7.29e-15	71.2
Msa1234690	MtrunA17_Chr4g0061421	53.731	67	29	1	12	78	10	74	1.09e-14	72.8
Msa1234690	MtrunA17_Chr4g0006391	46.377	69	32	1	10	78	4	67	4.57e-14	70.5
Msa1234690	MtrunA17_Chr2g0282741	43.478	69	34	1	10	78	4	67	6.96e-14	70.1
Msa1234690	MtrunA17_Chr4g0040501	44.928	69	33	1	10	78	4	67	1.09e-13	69.3
Msa1234690	MtrunA17_Chr3g0138471	43.478	69	34	1	10	78	4	67	4.45e-13	67.4
Msa1234690	MtrunA17_Chr5g0427221	40.741	81	38	2	7	83	45	119	6.44e-13	64.3
Msa1234690	MtrunA17_Chr7g0258061	42.857	84	41	2	4	86	16	93	9.66e-13	66.2
Msa1234690	MtrunA17_Chr1g0173931	42.500	80	39	2	8	86	36	109	1.60e-12	65.5
Msa1234690	MtrunA17_Chr1g0209551	40.476	84	44	1	9	92	15	92	2.01e-12	65.9
Msa1234690	MtrunA17_Chr7g0269841	50.000	68	28	2	11	78	27	88	5.42e-12	64.3
Msa1234690	MtrunA17_Chr8g0378161	44.118	68	34	1	11	78	61	124	7.41e-12	62.4
Msa1234690	MtrunA17_Chr5g0399201	42.857	70	37	1	10	79	12	78	9.16e-12	64.3
Msa1234690	MtrunA17_Chr2g0295821	41.250	80	40	2	1	80	95	167	3.82e-11	62.0
Msa1234690	MtrunA17_Chr3g0123861	44.928	69	31	1	10	78	82	143	6.56e-11	61.6
Msa1234690	MtrunA17_Chr6g0460381	48.485	66	31	2	10	74	3	66	7.52e-11	59.7
Msa1234690	MtrunA17_Chr6g0460411	47.143	70	34	2	10	78	2	69	9.06e-11	59.7
Msa1234690	MtrunA17_Chr6g0460461	47.143	70	34	2	10	78	2	69	9.10e-11	59.7
Msa1234690	MtrunA17_Chr5g0415731	38.889	72	42	1	9	78	4	75	9.18e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1234690	AT3G14200.1	50.649	231	93	8	10	234	12	227	8.53e-61	191
Msa1234690	AT1G72416.4	63.208	106	25	3	10	110	5	101	1.41e-38	132
Msa1234690	AT1G72416.2	64.423	104	23	3	10	108	5	99	1.54e-38	132
Msa1234690	AT1G72416.1	64.423	104	23	3	10	108	5	99	2.11e-38	132
Msa1234690	AT1G72416.3	64.423	104	23	3	10	108	5	99	5.54e-38	131
Msa1234690	AT1G56300.1	50.376	133	58	3	1	130	1	128	2.39e-34	120
Msa1234690	AT1G71000.1	46.897	145	60	6	4	140	2	137	7.65e-32	114
Msa1234690	AT1G56300.2	52.727	110	47	2	1	107	1	108	3.04e-29	106
Msa1234690	AT1G71000.2	41.104	163	81	5	4	160	2	155	3.58e-27	102
Msa1234690	AT1G72416.5	72.000	50	13	1	61	110	10	58	5.54e-17	75.9
Msa1234690	AT5G01390.3	49.275	69	30	1	10	78	4	67	4.73e-15	72.4
Msa1234690	AT5G01390.2	49.275	69	30	1	10	78	4	67	8.76e-15	72.4
Msa1234690	AT1G59725.1	46.377	69	33	1	10	78	4	68	1.11e-14	72.4
Msa1234690	AT5G01390.1	49.275	69	30	1	10	78	4	67	1.41e-14	72.4
Msa1234690	AT5G49060.2	46.914	81	35	3	7	86	96	169	1.53e-14	72.4
Msa1234690	AT5G49060.1	46.914	81	35	3	7	86	96	169	1.53e-14	72.4
Msa1234690	AT5G01390.4	49.275	69	30	1	10	78	4	67	2.72e-14	70.1
Msa1234690	AT5G03160.1	46.667	75	35	1	6	80	366	435	4.33e-14	71.2
Msa1234690	AT5G25530.2	44.928	69	33	1	10	78	4	67	6.62e-14	70.5
Msa1234690	AT4G28480.2	46.377	69	32	1	10	78	4	67	8.98e-14	69.7
Msa1234690	AT3G08910.1	46.377	69	32	1	10	78	4	67	1.13e-13	69.7
Msa1234690	AT4G28480.1	46.377	69	32	1	10	78	4	67	1.47e-13	69.3
Msa1234690	AT2G35720.1	42.308	78	42	1	2	79	7	81	2.35e-13	69.3
Msa1234690	AT3G47940.1	42.857	70	36	1	10	79	4	69	2.54e-13	68.9
Msa1234690	AT1G10350.1	42.029	69	35	1	10	78	4	67	4.20e-13	68.2
Msa1234690	AT5G16650.1	39.024	82	43	2	3	83	4	79	6.57e-13	64.3
Msa1234690	AT5G06910.1	38.525	122	59	5	1	117	19	129	7.79e-13	67.0
Msa1234690	AT2G20560.1	44.928	69	33	1	10	78	4	67	9.50e-13	67.0
Msa1234690	AT3G12170.1	38.739	111	55	5	1	105	1	104	2.11e-12	65.5
Msa1234690	AT5G16650.2	39.024	82	43	2	3	83	4	79	2.67e-12	63.5
Msa1234690	AT2G33735.1	50.704	71	25	2	10	78	22	84	3.32e-12	62.0
Msa1234690	AT2G33735.2	50.704	71	25	2	10	78	22	84	3.32e-12	62.0
Msa1234690	AT3G12170.2	37.838	111	56	5	1	105	37	140	5.31e-12	64.7
Msa1234690	AT4G39960.1	39.560	91	47	2	9	98	84	167	2.38e-11	63.2
Msa1234690	AT4G39960.2	39.560	91	47	2	9	98	84	167	2.38e-11	63.2
Msa1234690	AT5G25530.1	40.260	77	33	2	10	78	4	75	2.45e-11	62.8
Msa1234690	AT1G80030.4	42.254	71	34	1	8	78	73	136	5.35e-11	62.4
Msa1234690	AT1G80030.2	42.254	71	34	1	8	78	73	136	6.53e-11	62.0
Msa1234690	AT1G80030.3	42.254	71	34	1	8	78	73	136	6.53e-11	62.0
Msa1234690	AT1G80030.1	42.254	71	34	1	8	78	73	136	6.53e-11	62.0
Msa1234690	AT1G28210.3	31.973	147	84	5	8	150	46	180	8.32e-11	61.6
Msa1234690	AT1G28210.1	31.973	147	84	5	8	150	46	180	8.44e-11	61.6
Msa1234690	AT3G62600.1	48.529	68	29	2	11	78	27	88	8.83e-11	61.2

Find 67 sgRNAs with CRISPR-Local

Find 264 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAGAAGTTCTGGTAATT+TGG	0.142853	8_2:-12331679	None:intergenic
AATTCTACTCAGATGAATTA+TGG	0.200136	8_2:+12331628	Msa1234690:CDS
TCTAGTTGGTTGTGAAAATT+TGG	0.205516	8_2:+12329135	Msa1234690:five_prime_UTR
TGTTATTGTTGGAAATAATA+TGG	0.247286	8_2:+12329160	Msa1234690:exon
ATTAGTCATTTGTTGCATTA+TGG	0.247313	8_2:+12333028	Msa1234690:three_prime_UTR
GAAGATGTGGAGAAGGAAAA+AGG	0.260664	8_2:+12332583	Msa1234690:CDS
CCTTCTCCACATCTTCTTGA+AGG	0.267807	8_2:-12332576	None:intergenic
GTGTGTCAACCTTAGCATTT+TGG	0.273693	8_2:+12332743	Msa1234690:three_prime_UTR
CAATGATTTCTATGCAGTTT+TGG	0.279803	8_2:+12329202	Msa1234690:CDS
CCAGATCGTTGTTCAGCTTC+AGG	0.283002	8_2:+12329546	Msa1234690:CDS
AATTACCAGAACTTCTGTTT+TGG	0.283009	8_2:+12331682	Msa1234690:CDS
GGCTCACATTTGATTTAGTT+TGG	0.297419	8_2:-12330864	None:intergenic
CAAGCGGATATCGGATTAAA+TGG	0.298600	8_2:+12331523	Msa1234690:CDS
GCAGAGGATTCTTCTAGTTT+TGG	0.305479	8_2:+12331655	Msa1234690:CDS
AGCACCTCTCTTAATGCTTC+GGG	0.329574	8_2:+12331547	Msa1234690:CDS
CTTAAACTCAAACTCCTAGA+TGG	0.329984	8_2:-12332920	None:intergenic
CTTCAACTTGGATGACAACT+TGG	0.330506	8_2:-12328886	None:intergenic
CTAATGTTTCTTTGTATTGT+CGG	0.332006	8_2:-12329064	None:intergenic
TTATATGTGATACAGGAAAA+TGG	0.333099	8_2:+12331454	Msa1234690:intron
TATATAAAGTCTAGAATTAG+AGG	0.347820	8_2:+12328753	Msa1234690:five_prime_UTR
GCAATTCAAGAAGCCTATTC+TGG	0.348966	8_2:+12329609	Msa1234690:CDS
AGAAGCTAAGAAGAAATTTC+AGG	0.357982	8_2:+12329587	Msa1234690:CDS
TCATCTACTTTCATGACTTT+CGG	0.362653	8_2:+12331580	Msa1234690:CDS
AAATGCCTTGGAAAATTTGA+TGG	0.373884	8_2:+12332867	Msa1234690:three_prime_UTR
GTTTGGTTGGTTGATCTAGT+TGG	0.390684	8_2:+12329121	Msa1234690:five_prime_UTR
AAGCACCTCTCTTAATGCTT+CGG	0.391365	8_2:+12331546	Msa1234690:CDS
GATATGTTTCAAGCGGATAT+CGG	0.410739	8_2:+12331514	Msa1234690:CDS
TCTGTTGGAGAATTCTAGTT+TGG	0.411390	8_2:+12329104	Msa1234690:five_prime_UTR
ACATTAGTTTGATATTTGAT+TGG	0.420967	8_2:+12329080	Msa1234690:five_prime_UTR
ATTCTACTCAGATGAATTAT+GGG	0.423184	8_2:+12331629	Msa1234690:CDS
TGACCCCAAAACAGAAGTTC+TGG	0.428777	8_2:-12331687	None:intergenic
AAAATTTGGATTGTTATTGT+TGG	0.435217	8_2:+12329149	Msa1234690:five_prime_UTR
CAGATCGTTGTTCAGCTTCA+GGG	0.447334	8_2:+12329547	Msa1234690:CDS
AAGCAATTGTACACCCATCT+AGG	0.450605	8_2:+12332906	Msa1234690:three_prime_UTR
TGCATGTAAATTACCAGAAT+AGG	0.452286	8_2:-12329622	None:intergenic
AAATGGAGAGGAGAGCTTTG+AGG	0.455188	8_2:+12331471	Msa1234690:CDS
TATCAATTAGCAGAAATTCC+AGG	0.462490	8_2:-12332697	None:intergenic
CCTGAAGCTGAACAACGATC+TGG	0.468892	8_2:-12329546	None:intergenic
AATTCCAGGATGGTCATTAG+AGG	0.478304	8_2:-12332683	None:intergenic
GGGAGACTTTCTGAACGAAA+TGG	0.482099	8_2:+12330828	Msa1234690:CDS
TTGGATGACAACTTGGGTTG+CGG	0.483218	8_2:-12328879	None:intergenic
TACTAAGATAAGATAAACTA+TGG	0.485649	8_2:-12332823	None:intergenic
GGAAATAATATGGCTAATGA+AGG	0.487826	8_2:+12329170	Msa1234690:exon
TTGGAGAATTCTAGTTTGGT+TGG	0.496008	8_2:+12329108	Msa1234690:five_prime_UTR
TTCCCATGACCAAAATGCTA+AGG	0.508517	8_2:-12332752	None:intergenic
TGATATTTGATTGGTTCTGT+TGG	0.511150	8_2:+12329089	Msa1234690:five_prime_UTR
AATGCTCTGATTCAGAACTA+AGG	0.513112	8_2:+12329237	Msa1234690:CDS
TTCAGGGAATGTGAAGTTTG+TGG	0.516942	8_2:+12329563	Msa1234690:CDS
ATGGGTGTACAATTGCTTGA+AGG	0.522891	8_2:-12332901	None:intergenic
TTAGGAGGTCACAAATGCCT+TGG	0.526911	8_2:+12332855	Msa1234690:three_prime_UTR
AGTCATGAAAGTAGATGAAG+TGG	0.538936	8_2:-12331575	None:intergenic
TGCTTATAAGAAACTTGCAC+TGG	0.543386	8_2:+12329262	Msa1234690:CDS
TTCAACTTGGATGACAACTT+GGG	0.545053	8_2:-12328885	None:intergenic
ATTAATATACAACTTCAACT+TGG	0.554791	8_2:-12328898	None:intergenic
TTAAACTCAAACTCCTAGAT+GGG	0.559329	8_2:-12332919	None:intergenic
GTTTCCTCTAATGACCATCC+TGG	0.561659	8_2:+12332679	Msa1234690:CDS
ATTTGATGATATGTTTCAAG+CGG	0.571158	8_2:+12331507	Msa1234690:CDS
TTTAGACTAGATGGAATGAA+GGG	0.573998	8_2:-12328815	None:intergenic
CCTTCAAGAAGATGTGGAGA+AGG	0.586502	8_2:+12332576	Msa1234690:CDS
GAAGCACCTTCAAGAAGATG+TGG	0.587778	8_2:+12332570	Msa1234690:CDS
GATGAATTATGGGAAGGCAG+AGG	0.603296	8_2:+12331639	Msa1234690:CDS
TACTCAGATGAATTATGGGA+AGG	0.609702	8_2:+12331633	Msa1234690:CDS
TGTGATACAGGAAAATGGAG+AGG	0.651222	8_2:+12331459	Msa1234690:intron
AAGAGATTAATGTACGACGT+TGG	0.670458	8_2:+12329789	Msa1234690:CDS
AATTAGCAGAAATTCCAGGA+TGG	0.683865	8_2:-12332693	None:intergenic
GCAACCCGAAGCATTAAGAG+AGG	0.689028	8_2:-12331551	None:intergenic
AGGTCTCTAGAGTATTCATG+AGG	0.690314	8_2:+12328773	Msa1234690:five_prime_UTR

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTGATTATATGAAAAAAAT+CGG	-	chr8_2:12330426-12330445	None:intergenic	10.0%
!!	TATTGCAAAAATATTAAATA+GGG	-	chr8_2:12330888-12330907	None:intergenic	10.0%
!!	ATATTGCAAAAATATTAAAT+AGG	-	chr8_2:12330889-12330908	None:intergenic	10.0%
!!!	TTTATATATTTTTTGTTTAG+TGG	+	chr8_2:12330985-12331004	Msa1234690:intron	10.0%
!!	TATTATTTGAAAATAATTGT+TGG	+	chr8_2:12331222-12331241	Msa1234690:intron	10.0%
!!!	TGAATAAACTTTAGAAAAAA+CGG	-	chr8_2:12329360-12329379	None:intergenic	15.0%
!!	AGGAAAATAATTTGATTAAA+TGG	+	chr8_2:12329510-12329529	Msa1234690:intron	15.0%
!!	ACAAATAATAATTTGAATCA+TGG	-	chr8_2:12329705-12329724	None:intergenic	15.0%
!!!	TTCTCAATCTATTTTTTAAA+CGG	-	chr8_2:12330140-12330159	None:intergenic	15.0%
!!	TTGATTATATGAAAAAAATC+GGG	-	chr8_2:12330425-12330444	None:intergenic	15.0%
!!	AACTTGAATTAATCAATTTA+TGG	-	chr8_2:12331341-12331360	None:intergenic	15.0%
!!!	CTTTAAATTATACAAGATTT+TGG	+	chr8_2:12331909-12331928	Msa1234690:intron	15.0%
!!!	TCTATAAATGCAATTTTTTT+AGG	+	chr8_2:12332479-12332498	Msa1234690:intron	15.0%
!!!	TATATAAAGTCTAGAATTAG+AGG	+	chr8_2:12328753-12328772	Msa1234690:five_prime_UTR	20.0%
!!	ATTAATATACAACTTCAACT+TGG	-	chr8_2:12328901-12328920	None:intergenic	20.0%
!!!	GTTTCTTACTTTTTATGTTA+AGG	+	chr8_2:12328996-12329015	Msa1234690:five_prime_UTR	20.0%
!!!	ACGAATTTTGTGTTTTTATT+TGG	+	chr8_2:12329023-12329042	Msa1234690:five_prime_UTR	20.0%
!!!	TTTTGTGTTTTTATTTGGTT+TGG	+	chr8_2:12329028-12329047	Msa1234690:five_prime_UTR	20.0%
!!!	ACATTAGTTTGATATTTGAT+TGG	+	chr8_2:12329080-12329099	Msa1234690:five_prime_UTR	20.0%
!!!	AAAATTTGGATTGTTATTGT+TGG	+	chr8_2:12329149-12329168	Msa1234690:five_prime_UTR	20.0%
!!	TGTTATTGTTGGAAATAATA+TGG	+	chr8_2:12329160-12329179	Msa1234690:exon	20.0%
!!!	TTTTCTAAAGTTTATTCACA+TGG	+	chr8_2:12329362-12329381	Msa1234690:intron	20.0%
!!!	TTTCTAAAGTTTATTCACAT+GGG	+	chr8_2:12329363-12329382	Msa1234690:intron	20.0%
!!	TATCTTTCTTTCACAAATTT+AGG	-	chr8_2:12329440-12329459	None:intergenic	20.0%
!!!	TTTGTGAAAGAAAGATATTT+TGG	+	chr8_2:12329443-12329462	Msa1234690:intron	20.0%
!!!	AATGTAAATGGTGTTTTTTT+TGG	+	chr8_2:12329744-12329763	Msa1234690:intron	20.0%
!!!	ATGTAAATGGTGTTTTTTTT+GGG	+	chr8_2:12329745-12329764	Msa1234690:intron	20.0%
!!	AAAGTGAAAAGGTAAAATTA+AGG	-	chr8_2:12329967-12329986	None:intergenic	20.0%
!!	ATTCAGTTTCAATATTCATT+AGG	-	chr8_2:12330019-12330038	None:intergenic	20.0%
!!	ATGAATATTGAAACTGAATT+AGG	+	chr8_2:12330020-12330039	Msa1234690:intron	20.0%
!!	TGAATATTGAAACTGAATTA+GGG	+	chr8_2:12330021-12330040	Msa1234690:intron	20.0%
!!!	TGGAAATGAGTTAATTTTTT+AGG	+	chr8_2:12330240-12330259	Msa1234690:intron	20.0%
!!!	GTTTAAAATAGTGACTTAAT+TGG	-	chr8_2:12330310-12330329	None:intergenic	20.0%
!!	TTATAGCACATCAAAATTAT+TGG	-	chr8_2:12330776-12330795	None:intergenic	20.0%
!!!	AAAATGAATTTTGTTTTGCA+GGG	+	chr8_2:12330802-12330821	Msa1234690:intron	20.0%
!!	AACAAGACATAAACATTATT+AGG	-	chr8_2:12331087-12331106	None:intergenic	20.0%
!!!	AATAATGTTTATGTCTTGTT+TGG	+	chr8_2:12331087-12331106	Msa1234690:intron	20.0%
!!	ATTTGAAAATAATTGTTGGA+AGG	+	chr8_2:12331226-12331245	Msa1234690:intron	20.0%
!!!	AGTTAAAAGGATTGAAATTT+TGG	-	chr8_2:12331393-12331412	None:intergenic	20.0%
!!	AATGAATTGAAAGAGTTAAA+AGG	-	chr8_2:12331406-12331425	None:intergenic	20.0%
!!	GATTAATTTATATGTGATAC+AGG	+	chr8_2:12331447-12331466	Msa1234690:intron	20.0%
!!	TTTAACATGTAATTGAGTTA+TGG	+	chr8_2:12331880-12331899	Msa1234690:intron	20.0%
!!	TTAACATGTAATTGAGTTAT+GGG	+	chr8_2:12331881-12331900	Msa1234690:intron	20.0%
!!!	ATGATAGAAGATTTAGAATT+TGG	+	chr8_2:12331942-12331961	Msa1234690:intron	20.0%
!!	ACTCTATTAATAACTCAAAA+TGG	-	chr8_2:12332380-12332399	None:intergenic	20.0%
!!!	ATCCAACATTTTTTTATTCT+TGG	+	chr8_2:12332534-12332553	Msa1234690:intron	20.0%
!!	TACCAAGAATAAAAAAATGT+TGG	-	chr8_2:12332539-12332558	None:intergenic	20.0%
!!	ATGTACAACTAAAAAGATTA+GGG	+	chr8_2:12332794-12332813	Msa1234690:three_prime_UTR	20.0%
!!	TACTAAGATAAGATAAACTA+TGG	-	chr8_2:12332826-12332845	None:intergenic	20.0%
!!	ATCTTAGTACTAAAAAGATT+AGG	+	chr8_2:12332837-12332856	Msa1234690:three_prime_UTR	20.0%
!!!	AATGTCAATTTTGATTTGAA+AGG	+	chr8_2:12333061-12333080	Msa1234690:three_prime_UTR	20.0%
!	CTAATGTTTCTTTGTATTGT+CGG	-	chr8_2:12329067-12329086	None:intergenic	25.0%
!!	AATGATTTCTATGCAGTTTT+GGG	+	chr8_2:12329203-12329222	Msa1234690:CDS	25.0%
!!	ATAAACTTTAGAAAAAACGG+TGG	-	chr8_2:12329357-12329376	None:intergenic	25.0%
!!	ATTGTTGTTATGATGAAAGT+TGG	+	chr8_2:12329475-12329494	Msa1234690:intron	25.0%
!	AAAGTTGGAATTGTTTAGAA+AGG	+	chr8_2:12329490-12329509	Msa1234690:intron	25.0%
!	TTTGATTAAATGGACAGAAA+TGG	+	chr8_2:12329520-12329539	Msa1234690:intron	25.0%
!!	TATTGGGTCTAAAATGTAAA+TGG	+	chr8_2:12329732-12329751	Msa1234690:intron	25.0%
!	CATAACACAAAAATAGAAGT+AGG	-	chr8_2:12329902-12329921	None:intergenic	25.0%
!!	TTGTTTCTTCATTTACTTGA+AGG	+	chr8_2:12329924-12329943	Msa1234690:intron	25.0%
!	TGTTTCTTCATTTACTTGAA+GGG	+	chr8_2:12329925-12329944	Msa1234690:intron	25.0%
!	TTGTTAATGTGAAAGTGAAA+AGG	-	chr8_2:12329978-12329997	None:intergenic	25.0%
!!	CACTTTCACATTAACAATTT+TGG	+	chr8_2:12329981-12330000	Msa1234690:intron	25.0%
!!	TATAAGGATGATTTGATAGT+TGG	+	chr8_2:12330045-12330064	Msa1234690:intron	25.0%
!	AACAATGATATGGTCAATTT+CGG	+	chr8_2:12330195-12330214	Msa1234690:intron	25.0%
!	CAACGAAAACAAGAAAAAAA+TGG	+	chr8_2:12330220-12330239	Msa1234690:intron	25.0%
!!!	AGTCACTATTTTAAACCTAA+AGG	+	chr8_2:12330314-12330333	Msa1234690:intron	25.0%
!	AAGAGGAGAAGAAAAATAAT+TGG	-	chr8_2:12330528-12330547	None:intergenic	25.0%
!	AAAAAGAGATATGTTCTTCA+AGG	+	chr8_2:12330556-12330575	Msa1234690:intron	25.0%
!!!	AACATTTTTTTTCCTCCTTT+GGG	-	chr8_2:12330618-12330637	None:intergenic	25.0%
!!!	AAACATTTTTTTTCCTCCTT+TGG	-	chr8_2:12330619-12330638	None:intergenic	25.0%
!	GATTCAATTCCATGAATTAT+TGG	-	chr8_2:12330732-12330751	None:intergenic	25.0%
!	ATTCATGGAATTGAATCAAA+TGG	+	chr8_2:12330735-12330754	Msa1234690:intron	25.0%
!!!	GAAAATGAATTTTGTTTTGC+AGG	+	chr8_2:12330801-12330820	Msa1234690:intron	25.0%
!!!	TTTTGTTTGCTTGTAATTAG+TGG	+	chr8_2:12330916-12330935	Msa1234690:intron	25.0%
!!	AATCAATTTATGGAAAGTAC+AGG	-	chr8_2:12331331-12331350	None:intergenic	25.0%
!	TTATATGTGATACAGGAAAA+TGG	+	chr8_2:12331454-12331473	Msa1234690:intron	25.0%
!!	ATTTGATGATATGTTTCAAG+CGG	+	chr8_2:12331507-12331526	Msa1234690:CDS	25.0%
!	AATTCTACTCAGATGAATTA+TGG	+	chr8_2:12331628-12331647	Msa1234690:CDS	25.0%
!	ATTCTACTCAGATGAATTAT+GGG	+	chr8_2:12331629-12331648	Msa1234690:CDS	25.0%
!	TTGAATGATTGTTTATTGTG+AGG	+	chr8_2:12331805-12331824	Msa1234690:intron	25.0%
!	AGTAGTAACAACAAAAAGAT+AGG	+	chr8_2:12331857-12331876	Msa1234690:intron	25.0%
!	TAACATGTAATTGAGTTATG+GGG	+	chr8_2:12331882-12331901	Msa1234690:intron	25.0%
!	TAATCTATCTTCAGAGTAAT+TGG	-	chr8_2:12332110-12332129	None:intergenic	25.0%
!	TTAAGTGGTTAAGGAAAAAA+TGG	+	chr8_2:12332127-12332146	Msa1234690:intron	25.0%
!	CACTAATTAAAAGACTCATT+GGG	-	chr8_2:12332175-12332194	None:intergenic	25.0%
!!!	TTTTGTTGTTCATGTTATCA+AGG	+	chr8_2:12332202-12332221	Msa1234690:intron	25.0%
!	ATTATCATTACCAATGAATG+AGG	+	chr8_2:12332237-12332256	Msa1234690:intron	25.0%
!	TTTGTACCAAATTCTCAAAT+TGG	-	chr8_2:12332409-12332428	None:intergenic	25.0%
!	AAAGACAGAGAATAATTTGA+TGG	+	chr8_2:12332426-12332445	Msa1234690:intron	25.0%
!!!	TGCAATTTTTTTAGGAAGAT+TGG	+	chr8_2:12332487-12332506	Msa1234690:intron	25.0%
!	AGAAAACAAAAAATCTCATG+TGG	+	chr8_2:12332649-12332668	Msa1234690:CDS	25.0%
!	CATGTACAACTAAAAAGATT+AGG	+	chr8_2:12332793-12332812	Msa1234690:three_prime_UTR	25.0%
!	TGTACAACTAAAAAGATTAG+GGG	+	chr8_2:12332795-12332814	Msa1234690:three_prime_UTR	25.0%
!	TTAGTACTAAAAAGATTAGG+AGG	+	chr8_2:12332840-12332859	Msa1234690:three_prime_UTR	25.0%
!!	ATTAGTCATTTGTTGCATTA+TGG	+	chr8_2:12333028-12333047	Msa1234690:three_prime_UTR	25.0%
	TTTAGACTAGATGGAATGAA+GGG	-	chr8_2:12328818-12328837	None:intergenic	30.0%
!	TTTTAGACTAGATGGAATGA+AGG	-	chr8_2:12328819-12328838	None:intergenic	30.0%
!!!	GCATTTTGTTTTAGACTAGA+TGG	-	chr8_2:12328827-12328846	None:intergenic	30.0%
!!	TGATATTTGATTGGTTCTGT+TGG	+	chr8_2:12329089-12329108	Msa1234690:five_prime_UTR	30.0%
	TCTAGTTGGTTGTGAAAATT+TGG	+	chr8_2:12329135-12329154	Msa1234690:five_prime_UTR	30.0%
	GGAAATAATATGGCTAATGA+AGG	+	chr8_2:12329170-12329189	Msa1234690:exon	30.0%
!	CAATGATTTCTATGCAGTTT+TGG	+	chr8_2:12329202-12329221	Msa1234690:CDS	30.0%
	AGAAGCTAAGAAGAAATTTC+AGG	+	chr8_2:12329587-12329606	Msa1234690:CDS	30.0%
	TGCATGTAAATTACCAGAAT+AGG	-	chr8_2:12329625-12329644	None:intergenic	30.0%
	TAATTTGAATCATGGCTCAT+GGG	-	chr8_2:12329697-12329716	None:intergenic	30.0%
	ATAATTTGAATCATGGCTCA+TGG	-	chr8_2:12329698-12329717	None:intergenic	30.0%
	GTTTCAATATTCATTAGGCA+TGG	-	chr8_2:12330014-12330033	None:intergenic	30.0%
	GAAACTGAATTAGGGATATA+AGG	+	chr8_2:12330029-12330048	Msa1234690:intron	30.0%
	GGAGAAGAAAAATAATTGGA+CGG	-	chr8_2:12330524-12330543	None:intergenic	30.0%
	ATATGTTCTTCAAGGTTAGT+AGG	+	chr8_2:12330564-12330583	Msa1234690:intron	30.0%
	TTTGGGAAAAGACATTTGTT+GGG	-	chr8_2:12330601-12330620	None:intergenic	30.0%
	CAATAAGAGCCAATAATTCA+TGG	+	chr8_2:12330720-12330739	Msa1234690:intron	30.0%
!!!	AATTTTGTTTTGCAGGGTAT+GGG	+	chr8_2:12330808-12330827	Msa1234690:intron	30.0%
	TAATTGTTGGAAGGATAAAG+TGG	+	chr8_2:12331235-12331254	Msa1234690:intron	30.0%
	TCATCTACTTTCATGACTTT+CGG	+	chr8_2:12331580-12331599	Msa1234690:CDS	30.0%
!	AAACAGAAGTTCTGGTAATT+TGG	-	chr8_2:12331682-12331701	None:intergenic	30.0%
!	AATTACCAGAACTTCTGTTT+TGG	+	chr8_2:12331682-12331701	Msa1234690:CDS	30.0%
!	ATTACCAGAACTTCTGTTTT+GGG	+	chr8_2:12331683-12331702	Msa1234690:CDS	30.0%
	ATGAATGTTGCATCAAATGA+AGG	-	chr8_2:12331743-12331762	None:intergenic	30.0%
	TTCATTTGATGCAACATTCA+TGG	+	chr8_2:12331742-12331761	Msa1234690:intron	30.0%
	AATCACAACAATTCATGCTT+CGG	-	chr8_2:12332073-12332092	None:intergenic	30.0%
!	TACTCTGAAGATAGATTAAG+TGG	+	chr8_2:12332112-12332131	Msa1234690:intron	30.0%
	GAAGATAGATTAAGTGGTTA+AGG	+	chr8_2:12332118-12332137	Msa1234690:intron	30.0%
	CCACTAATTAAAAGACTCAT+TGG	-	chr8_2:12332176-12332195	None:intergenic	30.0%
!!	CCAATGAGTCTTTTAATTAG+TGG	+	chr8_2:12332173-12332192	Msa1234690:intron	30.0%
!	TGTTTTGTATCCTCATTCAT+TGG	-	chr8_2:12332250-12332269	None:intergenic	30.0%
	TGAATGAGGATACAAAACAA+TGG	+	chr8_2:12332251-12332270	Msa1234690:intron	30.0%
	TCTCCTTACAAAACTAATAG+AGG	+	chr8_2:12332320-12332339	Msa1234690:intron	30.0%
	CTCCTTACAAAACTAATAGA+GGG	+	chr8_2:12332321-12332340	Msa1234690:intron	30.0%
	CACTCCATATATTATATAGC+TGG	+	chr8_2:12332451-12332470	Msa1234690:intron	30.0%
	GAGACCAGCTATATAATATA+TGG	-	chr8_2:12332458-12332477	None:intergenic	30.0%
	GGTAGCAAAAAACAAAGAAA+AGG	+	chr8_2:12332625-12332644	Msa1234690:CDS	30.0%
	TATCAATTAGCAGAAATTCC+AGG	-	chr8_2:12332700-12332719	None:intergenic	30.0%
	GTACAACTAAAAAGATTAGG+GGG	+	chr8_2:12332796-12332815	Msa1234690:three_prime_UTR	30.0%
	AAATGCCTTGGAAAATTTGA+TGG	+	chr8_2:12332867-12332886	Msa1234690:three_prime_UTR	30.0%
	TTAAACTCAAACTCCTAGAT+GGG	-	chr8_2:12332922-12332941	None:intergenic	30.0%
	TTCAACTTGGATGACAACTT+GGG	-	chr8_2:12328888-12328907	None:intergenic	35.0%
!	TCTGTTGGAGAATTCTAGTT+TGG	+	chr8_2:12329104-12329123	Msa1234690:five_prime_UTR	35.0%
!!	TTGGAGAATTCTAGTTTGGT+TGG	+	chr8_2:12329108-12329127	Msa1234690:five_prime_UTR	35.0%
!!!	TGCAGTTTTGGGATTGAATA+AGG	+	chr8_2:12329214-12329233	Msa1234690:CDS	35.0%
	AATGCTCTGATTCAGAACTA+AGG	+	chr8_2:12329237-12329256	Msa1234690:CDS	35.0%
	TGCTTATAAGAAACTTGCAC+TGG	+	chr8_2:12329262-12329281	Msa1234690:CDS	35.0%
	GGTTAGAGTAGAAACAAAGA+CGG	-	chr8_2:12329329-12329348	None:intergenic	35.0%
!	ACTTTAGAAAAAACGGTGGA+GGG	-	chr8_2:12329353-12329372	None:intergenic	35.0%
!	AACTTTAGAAAAAACGGTGG+AGG	-	chr8_2:12329354-12329373	None:intergenic	35.0%
	AAGAGATTAATGTACGACGT+TGG	+	chr8_2:12329789-12329808	Msa1234690:CDS	35.0%
	ATTTCACACCATTCATGAGT+TGG	+	chr8_2:12329854-12329873	Msa1234690:intron	35.0%
	TTTCACACCATTCATGAGTT+GGG	+	chr8_2:12329855-12329874	Msa1234690:intron	35.0%
!	AAGGATGATTTGATAGTTGG+TGG	+	chr8_2:12330048-12330067	Msa1234690:intron	35.0%
!	AGGATGATTTGATAGTTGGT+GGG	+	chr8_2:12330049-12330068	Msa1234690:intron	35.0%
	TTAAAGATCAGAGTGATGAG+CGG	+	chr8_2:12330076-12330095	Msa1234690:intron	35.0%
	TGAGAATGAATTTCCTCTCT+TGG	+	chr8_2:12330154-12330173	Msa1234690:intron	35.0%
	TTGACCATATCATTGTTTCG+TGG	-	chr8_2:12330192-12330211	None:intergenic	35.0%
	TCACTGTCTTCTTTACCTTT+AGG	-	chr8_2:12330332-12330351	None:intergenic	35.0%
!!!	TCTCTTTTTTCACGTGAAAG+AGG	-	chr8_2:12330545-12330564	None:intergenic	35.0%
!	TTGGGAAAAGACATTTGTTG+GGG	-	chr8_2:12330600-12330619	None:intergenic	35.0%
!	CTTTGGGAAAAGACATTTGT+TGG	-	chr8_2:12330602-12330621	None:intergenic	35.0%
!	CAACAAATGTCTTTTCCCAA+AGG	+	chr8_2:12330600-12330619	Msa1234690:intron	35.0%
	TATGTGTCACATAACACATG+TGG	+	chr8_2:12330678-12330697	Msa1234690:intron	35.0%
	CATGGAATTGAATCAAATGG+AGG	+	chr8_2:12330738-12330757	Msa1234690:intron	35.0%
!!!	GAATTTTGTTTTGCAGGGTA+TGG	+	chr8_2:12330807-12330826	Msa1234690:intron	35.0%
!	GGCTCACATTTGATTTAGTT+TGG	-	chr8_2:12330867-12330886	None:intergenic	35.0%
	CTTGTAATTAGTGGAACGTT+AGG	+	chr8_2:12330925-12330944	Msa1234690:intron	35.0%
	AATTAGTGGAACGTTAGGTT+AGG	+	chr8_2:12330930-12330949	Msa1234690:intron	35.0%
	AGGCACAACTACATAGTATT+AGG	-	chr8_2:12331043-12331062	None:intergenic	35.0%
!	AGTTGTGCCTTTTTACCTTT+TGG	+	chr8_2:12331053-12331072	Msa1234690:intron	35.0%
!	GTTGTGCCTTTTTACCTTTT+GGG	+	chr8_2:12331054-12331073	Msa1234690:intron	35.0%
!!!	CCTTTTTACCTTTTGGGATT+GGG	+	chr8_2:12331060-12331079	Msa1234690:intron	35.0%
	ACACCAACTATCATAGCAAT+TGG	-	chr8_2:12331114-12331133	None:intergenic	35.0%
!	AATTGCTATGATAGTTGGTG+TGG	+	chr8_2:12331113-12331132	Msa1234690:intron	35.0%
	GATATGTTTCAAGCGGATAT+CGG	+	chr8_2:12331514-12331533	Msa1234690:CDS	35.0%
	AGTCATGAAAGTAGATGAAG+TGG	-	chr8_2:12331578-12331597	None:intergenic	35.0%
	TACTCAGATGAATTATGGGA+AGG	+	chr8_2:12331633-12331652	Msa1234690:CDS	35.0%
!	TTACCAGAACTTCTGTTTTG+GGG	+	chr8_2:12331684-12331703	Msa1234690:CDS	35.0%
	GGCAAACATATAGCATAGTA+AGG	+	chr8_2:12331763-12331782	Msa1234690:intron	35.0%
	TGTTACTACTTACTACCCAT+AGG	-	chr8_2:12331847-12331866	None:intergenic	35.0%
	TATCTTCAGAGTAATTGGCA+AGG	-	chr8_2:12332105-12332124	None:intergenic	35.0%
	ACAAAACAATGGAGTATGTG+TGG	+	chr8_2:12332262-12332281	Msa1234690:intron	35.0%
	ATGTGTGGAGTATATAGAAG+TGG	+	chr8_2:12332277-12332296	Msa1234690:intron	35.0%
!!	AGTTTTGTAAGGAGATCACT+AGG	-	chr8_2:12332315-12332334	None:intergenic	35.0%
!!!	GTCCCTCTATTAGTTTTGTA+AGG	-	chr8_2:12332326-12332345	None:intergenic	35.0%
	TTGTCAAACGTTTCTCAGAT+TGG	-	chr8_2:12332348-12332367	None:intergenic	35.0%
	CAGAGACCAATTTGAGAATT+TGG	+	chr8_2:12332400-12332419	Msa1234690:intron	35.0%
	CAGTATCAAGTTAAATGCAG+TGG	-	chr8_2:12332514-12332533	None:intergenic	35.0%
	AAGATGTGGAGAAGGAAAAA+GGG	+	chr8_2:12332584-12332603	Msa1234690:CDS	35.0%
	AATTAGCAGAAATTCCAGGA+TGG	-	chr8_2:12332696-12332715	None:intergenic	35.0%
!!	AACCTTAGCATTTTGGTCAT+GGG	+	chr8_2:12332750-12332769	Msa1234690:three_prime_UTR	35.0%
!	CACTACCATCAAATTTTCCA+AGG	-	chr8_2:12332875-12332894	None:intergenic	35.0%
	CTTAAACTCAAACTCCTAGA+TGG	-	chr8_2:12332923-12332942	None:intergenic	35.0%
!!	AGGTCTCTAGAGTATTCATG+AGG	+	chr8_2:12328773-12328792	Msa1234690:five_prime_UTR	40.0%
	CTTCAACTTGGATGACAACT+TGG	-	chr8_2:12328889-12328908	None:intergenic	40.0%
!	GTTTGGTTGGTTGATCTAGT+TGG	+	chr8_2:12329121-12329140	Msa1234690:five_prime_UTR	40.0%
	TTCAGGGAATGTGAAGTTTG+TGG	+	chr8_2:12329563-12329582	Msa1234690:CDS	40.0%
	GCAATTCAAGAAGCCTATTC+TGG	+	chr8_2:12329609-12329628	Msa1234690:CDS	40.0%
	AGGCAAGTGAAAGAGAAAGA+GGG	-	chr8_2:12329882-12329901	None:intergenic	40.0%
	TAGGCAAGTGAAAGAGAAAG+AGG	-	chr8_2:12329883-12329902	None:intergenic	40.0%
!	GGATGATTTGATAGTTGGTG+GGG	+	chr8_2:12330050-12330069	Msa1234690:intron	40.0%
	TAAAGATCAGAGTGATGAGC+GGG	+	chr8_2:12330077-12330096	Msa1234690:intron	40.0%
	GAAGCAAATATGACCAAGAG+AGG	-	chr8_2:12330170-12330189	None:intergenic	40.0%
	GCTTCCACGAAACAATGATA+TGG	+	chr8_2:12330185-12330204	Msa1234690:intron	40.0%
!	CAAATGTCTTTTCCCAAAGG+AGG	+	chr8_2:12330603-12330622	Msa1234690:intron	40.0%
!!!	GCCTTTTTACCTTTTGGGAT+TGG	+	chr8_2:12331059-12331078	Msa1234690:intron	40.0%
	CCCAATCCCAAAAGGTAAAA+AGG	-	chr8_2:12331063-12331082	None:intergenic	40.0%
	TATTAGGACCCAATCCCAAA+AGG	-	chr8_2:12331071-12331090	None:intergenic	40.0%
	GGTCCAATTGCTATGATAGT+TGG	+	chr8_2:12331108-12331127	Msa1234690:intron	40.0%
	GGAAGGATAAAGTGGTTTAC+TGG	+	chr8_2:12331243-12331262	Msa1234690:intron	40.0%
	GATAAAGTGGTTTACTGGAC+CGG	+	chr8_2:12331248-12331267	Msa1234690:intron	40.0%
	ATAAAGTGGTTTACTGGACC+GGG	+	chr8_2:12331249-12331268	Msa1234690:intron	40.0%
	TGTGATACAGGAAAATGGAG+AGG	+	chr8_2:12331459-12331478	Msa1234690:intron	40.0%
	CAAGCGGATATCGGATTAAA+TGG	+	chr8_2:12331523-12331542	Msa1234690:CDS	40.0%
	AAGCACCTCTCTTAATGCTT+CGG	+	chr8_2:12331546-12331565	Msa1234690:CDS	40.0%
!!!	GCAGAGGATTCTTCTAGTTT+TGG	+	chr8_2:12331655-12331674	Msa1234690:CDS	40.0%
	TGTGAGGTCCTTTACTTGTT+TGG	+	chr8_2:12331821-12331840	Msa1234690:intron	40.0%
	TCCTTTACTTGTTTGGCCTA+TGG	+	chr8_2:12331828-12331847	Msa1234690:intron	40.0%
	CCTTTACTTGTTTGGCCTAT+GGG	+	chr8_2:12331829-12331848	Msa1234690:intron	40.0%
	GATGCAACTATTGTAGTCGT+TGG	+	chr8_2:12331986-12332005	Msa1234690:intron	40.0%
!	AATGCACATTTGTTGGATCC+TGG	-	chr8_2:12332016-12332035	None:intergenic	40.0%
	TGCAACGAATGCACATTTGT+TGG	-	chr8_2:12332023-12332042	None:intergenic	40.0%
	ACAACAATTCATGCTTCGGA+TGG	-	chr8_2:12332069-12332088	None:intergenic	40.0%
	GAAGATGTGGAGAAGGAAAA+AGG	+	chr8_2:12332583-12332602	Msa1234690:CDS	40.0%
	AGATGTGGAGAAGGAAAAAG+GGG	+	chr8_2:12332585-12332604	Msa1234690:CDS	40.0%
	GAAAAAGGGGGAATTCTAGA+AGG	+	chr8_2:12332598-12332617	Msa1234690:CDS	40.0%
	AATTCCAGGATGGTCATTAG+AGG	-	chr8_2:12332686-12332705	None:intergenic	40.0%
!	GTGTGTCAACCTTAGCATTT+TGG	+	chr8_2:12332743-12332762	Msa1234690:three_prime_UTR	40.0%
!!	CAACCTTAGCATTTTGGTCA+TGG	+	chr8_2:12332749-12332768	Msa1234690:three_prime_UTR	40.0%
	TTCCCATGACCAAAATGCTA+AGG	-	chr8_2:12332755-12332774	None:intergenic	40.0%
	ATGGGTGTACAATTGCTTGA+AGG	-	chr8_2:12332904-12332923	None:intergenic	40.0%
	AAGCAATTGTACACCCATCT+AGG	+	chr8_2:12332906-12332925	Msa1234690:three_prime_UTR	40.0%
!	TTGGATGACAACTTGGGTTG+CGG	-	chr8_2:12328882-12328901	None:intergenic	45.0%
	TTAGAAAAAACGGTGGAGGG+TGG	-	chr8_2:12329350-12329369	None:intergenic	45.0%
	CAGATCGTTGTTCAGCTTCA+GGG	+	chr8_2:12329547-12329566	Msa1234690:CDS	45.0%
	GGGAGACTTTCTGAACGAAA+TGG	+	chr8_2:12330828-12330847	Msa1234690:CDS	45.0%
!	TGCTATGATAGTTGGTGTGG+TGG	+	chr8_2:12331116-12331135	Msa1234690:intron	45.0%
!	GCTATGATAGTTGGTGTGGT+GGG	+	chr8_2:12331117-12331136	Msa1234690:intron	45.0%
!	CTATGATAGTTGGTGTGGTG+GGG	+	chr8_2:12331118-12331137	Msa1234690:intron	45.0%
!	TATGATAGTTGGTGTGGTGG+GGG	+	chr8_2:12331119-12331138	Msa1234690:intron	45.0%
!	TGTGGTGGGGGTATATTGTT+GGG	+	chr8_2:12331131-12331150	Msa1234690:intron	45.0%
	TATTGTACGAACACACAGCC+CGG	-	chr8_2:12331270-12331289	None:intergenic	45.0%
	AAATGGAGAGGAGAGCTTTG+AGG	+	chr8_2:12331471-12331490	Msa1234690:CDS	45.0%
	AGCACCTCTCTTAATGCTTC+GGG	+	chr8_2:12331547-12331566	Msa1234690:CDS	45.0%
	GATGAATTATGGGAAGGCAG+AGG	+	chr8_2:12331639-12331658	Msa1234690:CDS	45.0%
	TGACCCCAAAACAGAAGTTC+TGG	-	chr8_2:12331690-12331709	None:intergenic	45.0%
	CCCATAGGCCAAACAAGTAA+AGG	-	chr8_2:12331832-12331851	None:intergenic	45.0%
!	GAAGCACCTTCAAGAAGATG+TGG	+	chr8_2:12332570-12332589	Msa1234690:CDS	45.0%
	CCTTCTCCACATCTTCTTGA+AGG	-	chr8_2:12332579-12332598	None:intergenic	45.0%
	CCTTCAAGAAGATGTGGAGA+AGG	+	chr8_2:12332576-12332595	Msa1234690:CDS	45.0%
	GATGTGGAGAAGGAAAAAGG+GGG	+	chr8_2:12332586-12332605	Msa1234690:CDS	45.0%
!	AAAGGGGGAATTCTAGAAGG+AGG	+	chr8_2:12332601-12332620	Msa1234690:CDS	45.0%
	GTTTCCTCTAATGACCATCC+TGG	+	chr8_2:12332679-12332698	Msa1234690:CDS	45.0%
	TTAGGAGGTCACAAATGCCT+TGG	+	chr8_2:12332855-12332874	Msa1234690:three_prime_UTR	45.0%
!!!	AAATTATATATTTTTTTTCA+TGG	+	chr8_2:12329394-12329413	Msa1234690:intron	5.0%
!!!	TATTATTTGTTTTTATATAT+TGG	+	chr8_2:12329715-12329734	Msa1234690:intron	5.0%
!!!	ATTATTTGTTTTTATATATT+GGG	+	chr8_2:12329716-12329735	Msa1234690:intron	5.0%
	CCTGAAGCTGAACAACGATC+TGG	-	chr8_2:12329549-12329568	None:intergenic	50.0%
	CCAGATCGTTGTTCAGCTTC+AGG	+	chr8_2:12329546-12329565	Msa1234690:CDS	50.0%
	AGAGGGTCCCAACTCATGAA+TGG	-	chr8_2:12329865-12329884	None:intergenic	50.0%
!	GTGTGGTGGGGGTATATTGT+TGG	+	chr8_2:12331130-12331149	Msa1234690:intron	50.0%
!	GTGGTGGGGGTATATTGTTG+GGG	+	chr8_2:12331132-12331151	Msa1234690:intron	50.0%
!	GCAACCCGAAGCATTAAGAG+AGG	-	chr8_2:12331554-12331573	None:intergenic	50.0%
	ATTGTAGTCGTTGGATCGCC+AGG	+	chr8_2:12331995-12332014	Msa1234690:intron	50.0%
	ATCCTCGACAACAGCAACAC+TGG	-	chr8_2:12332046-12332065	None:intergenic	50.0%
!	CACCAGTGTTGCTGTTGTCG+AGG	+	chr8_2:12332041-12332060	Msa1234690:intron	55.0%
!	GGGGGAATTCTAGAAGGAGG+AGG	+	chr8_2:12332604-12332623	Msa1234690:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	12328734	12333095	12328734	ID=Msa1234690;Name=Msa1234690
chr8_2	mRNA	12328734	12333095	12328734	ID=Msa1234690-mRNA-1;Parent=Msa1234690;Name=Msa1234690-mRNA-1;_AED=0.21;_eAED=0.21;_QI=445\|1\|1\|1\|1\|1\|6\|378\|237
chr8_2	exon	12328734	12329283	12328734	ID=Msa1234690-mRNA-1:exon:4754;Parent=Msa1234690-mRNA-1
chr8_2	exon	12329537	12329630	12329537	ID=Msa1234690-mRNA-1:exon:4755;Parent=Msa1234690-mRNA-1
chr8_2	exon	12329772	12329842	12329772	ID=Msa1234690-mRNA-1:exon:4756;Parent=Msa1234690-mRNA-1
chr8_2	exon	12330823	12330879	12330823	ID=Msa1234690-mRNA-1:exon:4757;Parent=Msa1234690-mRNA-1
chr8_2	exon	12331469	12331705	12331469	ID=Msa1234690-mRNA-1:exon:4758;Parent=Msa1234690-mRNA-1
chr8_2	exon	12332568	12333095	12332568	ID=Msa1234690-mRNA-1:exon:4759;Parent=Msa1234690-mRNA-1
chr8_2	five_prime_UTR	12328734	12329178	12328734	ID=Msa1234690-mRNA-1:five_prime_utr;Parent=Msa1234690-mRNA-1
chr8_2	CDS	12329179	12329283	12329179	ID=Msa1234690-mRNA-1:cds;Parent=Msa1234690-mRNA-1
chr8_2	CDS	12329537	12329630	12329537	ID=Msa1234690-mRNA-1:cds;Parent=Msa1234690-mRNA-1
chr8_2	CDS	12329772	12329842	12329772	ID=Msa1234690-mRNA-1:cds;Parent=Msa1234690-mRNA-1
chr8_2	CDS	12330823	12330879	12330823	ID=Msa1234690-mRNA-1:cds;Parent=Msa1234690-mRNA-1
chr8_2	CDS	12331469	12331705	12331469	ID=Msa1234690-mRNA-1:cds;Parent=Msa1234690-mRNA-1
chr8_2	CDS	12332568	12332717	12332568	ID=Msa1234690-mRNA-1:cds;Parent=Msa1234690-mRNA-1
chr8_2	three_prime_UTR	12332718	12333095	12332718	ID=Msa1234690-mRNA-1:three_prime_utr;Parent=Msa1234690-mRNA-1

Gene Sequence

>Msa1234690

ATGGCTAATGAAGGAAACAAAAGCAATGATTTCTATGCAGTTTTGGGATTGAATAAGGAATGCTCTGATTCAGAACTAAGGAATGCTTATAAGAAACTTGCACTGAAATGGCATCCAGATCGTTGTTCAGCTTCAGGGAATGTGAAGTTTGTGGAAGAAGCTAAGAAGAAATTTCAGGCAATTCAAGAAGCCTATTCTGTTTTATCTGACTCGAACAAGAGATTAATGTACGACGTTGGAGTTTACGACAACAGTGATGATGACGAAAATGGTATGGGAGACTTTCTGAACGAAATGGTTACAATGATGAGCCAAACTAAATCAAATGAAAATGGAGAGGAGAGCTTTGAGGAGTTGCAACAGTTATTTGATGATATGTTTCAAGCGGATATCGGATTAAATGGAAGCACCTCTCTTAATGCTTCGGGTTGCTCCACTTCATCTACTTTCATGACTTTCGGTGAAAGCGCGAATTCAAATAAGCGCAATTCTACTCAGATGAATTATGGGAAGGCAGAGGATTCTTCTAGTTTTGGTCCAAATTACCAGAACTTCTGTTTTGGGATGAAGCACCTTCAAGAAGATGTGGAGAAGGAAAAAGGGGGAATTCTAGAAGGAGGAGGTAGCAAAAAACAAAGAAAAGGAAGAAAACAAAAAATCTCATGTGGACATGATGTTTCCTCTAATGACCATCCTGGAATTTCTGCTAATTGA

Protein sequence

>Msa1234690

MANEGNKSNDFYAVLGLNKECSDSELRNAYKKLALKWHPDRCSASGNVKFVEEAKKKFQAIQEAYSVLSDSNKRLMYDVGVYDNSDDDENGMGDFLNEMVTMMSQTKSNENGEESFEELQQLFDDMFQADIGLNGSTSLNASGCSTSSTFMTFGESANSNKRNSTQMNYGKAEDSSSFGPNYQNFCFGMKHLQEDVEKEKGGILEGGGSKKQRKGRKQKISCGHDVSSNDHPGISAN*