AlfalfaGEDB | Msa0055750

M. sativa cultivar zhongmu-4 / Msa0055750

KEGG: -
GO: -
PFAM: PF13947.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0055750	sp\|Q9LMN7\|WAK5_ARATH	50.301	499	220	8	721	1200	244	733	8.02e-144	455
Msa0055750	sp\|Q9LMN7\|WAK5_ARATH	41.298	678	275	23	39	613	33	690	2.43e-137	438

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0055750	A0A396JKP2	56.742	749	198	18	1	638	3	736	0.0	777
Msa0055750	A0A396JKP2	82.278	79	14	0	638	716	14	92	8.13e-32	145
Msa0055750	A0A396JKP2	88.577	499	42	1	717	1200	263	761	0.0	910

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0055710	Msa0055750	0.809607	1.740399e-50	-8.615850e-47
Msa0055720	Msa0055750	0.964621	1.473211e-123	-8.615850e-47
Msa0055750	Msa0165510	0.815777	7.770388e-52	-8.615850e-47
Msa0055750	Msa0227470	0.825765	3.921881e-54	-8.615850e-47
Msa0055750	Msa0243100	0.809888	1.514240e-50	-8.615850e-47
Msa0055750	Msa0243890	0.811365	7.261282e-51	-8.615850e-47
Msa0055750	Msa0282590	0.818271	2.138309e-52	-8.615850e-47
Msa0055750	Msa0311860	0.801989	6.938534e-49	-8.615850e-47
Msa0055750	Msa0410650	0.802931	4.439078e-49	-8.615850e-47
Msa0055750	Msa0414170	0.800516	1.389345e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0055750	MtrunA17_Chr1g0148501	88.577	499	42	1	717	1200	263	761	0.0	910
Msa0055750	MtrunA17_Chr1g0148501	56.742	749	198	18	1	638	3	736	0.0	777
Msa0055750	MtrunA17_Chr1g0148501	82.278	79	14	0	638	716	14	92	1.57e-35	145
Msa0055750	MtrunA17_Chr1g0148511	64.305	734	149	16	6	638	9	730	0.0	875
Msa0055750	MtrunA17_Chr1g0148511	77.014	509	90	4	718	1200	248	755	0.0	778
Msa0055750	MtrunA17_Chr1g0148461	83.550	462	55	2	717	1163	276	731	0.0	787
Msa0055750	MtrunA17_Chr1g0148461	57.143	728	183	19	6	626	26	731	0.0	743
Msa0055750	MtrunA17_Chr1g0148461	75.949	79	15	1	638	716	32	106	1.22e-30	130
Msa0055750	MtrunA17_Chr1g0148491	64.340	659	117	14	82	638	2	644	0.0	785
Msa0055750	MtrunA17_Chr1g0148491	75.432	521	101	7	698	1200	158	669	0.0	768

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0055750	AT1G21230.1	50.301	499	220	8	721	1200	244	733	8.15e-145	455
Msa0055750	AT1G21230.1	41.298	678	275	23	39	613	33	690	2.47e-138	438
Msa0055750	AT1G21250.1	49.600	500	221	10	721	1200	247	735	3.00e-139	440
Msa0055750	AT1G21250.1	38.934	732	306	23	3	627	6	703	2.32e-132	422
Msa0055750	AT1G21270.1	48.703	501	227	9	720	1200	242	732	9.54e-138	436
Msa0055750	AT1G21270.1	40.119	673	286	18	39	613	33	686	6.64e-136	431
Msa0055750	AT1G21240.1	47.782	496	231	8	721	1200	258	741	2.02e-137	436
Msa0055750	AT1G21240.1	40.057	704	278	26	39	627	35	709	5.46e-129	413
Msa0055750	AT1G21210.1	46.521	503	238	9	721	1200	244	738	4.65e-135	429
Msa0055750	AT1G21210.1	37.139	727	317	24	13	627	8	706	1.78e-123	398

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Find 236 sgRNAs with CRISPR-Local

Find 1170 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTGATGTCTATAGCTT+TGG	0.378017	1_2:-10927237	Msa0055750:CDS
AAAAGTGATGTCTATAGCTT+TGG	0.378017	1_2:-10938896	Msa0055750:CDS
AAAATGAACCTGTGCAGCCA+TGG	0.494057	1_2:+10940182	None:intergenic
AAACAAACAAGAGATCAAGG+AGG	0.753887	1_2:-10927067	Msa0055750:CDS
AAACAAACAAGAGATCAAGG+AGG	0.782860	1_2:-10938726	Msa0055750:CDS
AAATGAACCTGTGCAGCCAT+GGG	0.533498	1_2:+10940183	None:intergenic
AAATTCTTTAGGCAGAATGG+AGG	0.636740	1_2:-10927897	Msa0055750:CDS
AAATTCTTTGAGCAGAATGG+TGG	0.605947	1_2:-10939586	Msa0055750:CDS
AACAACTCAAAACTAATCCG+TGG	0.655250	1_2:-10933715	Msa0055750:CDS
AACACAACAAATTGACCTTC+AGG	0.412655	1_2:+10940781	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTAACAATTCCATATCCATT+TGG	-	chr1_2:10926927-10926946	Msa0055750:CDS	30.0%
AATTTACGATCCAAGTAACA+TGG	+	chr1_2:10926981-10927000	None:intergenic	30.0%
TAATTTCAGCATTTCAAGCA+AGG	-	chr1_2:10927154-10927173	Msa0055750:CDS	30.0%
TCCACTCATTTAGAAAGAAA+TGG	-	chr1_2:10927455-10927474	Msa0055750:CDS	30.0%
TCCATTTCTTTCTAAATGAG+TGG	+	chr1_2:10927459-10927478	None:intergenic	30.0%
AAGGACAATAGATAATACCA+TGG	+	chr1_2:10927991-10928010	None:intergenic	30.0%
ATGGTATTATCTATTGTCCT+TGG	-	chr1_2:10927990-10928009	Msa0055750:intron	30.0%
CATTAACGAATTGATCGATT+TGG	+	chr1_2:10928523-10928542	None:intergenic	30.0%
AATCGATCAATTCGTTAATG+AGG	-	chr1_2:10928523-10928542	Msa0055750:intron	30.0%
ACAAACTCATAAACCAACAA+AGG	+	chr1_2:10928620-10928639	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	10926804	10941070	10926804	ID=Msa0055750;Name=Msa0055750
chr1_2	mRNA	10926804	10941070	10926804	ID=Msa0055750-mRNA-1;Parent=Msa0055750;Name=Msa0055750-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0\|0\|0\|1\|0.23\|0.14\|14\|0\|1200
chr1_2	exon	10940839	10941070	10940839	ID=Msa0055750-mRNA-1:exon:4172;Parent=Msa0055750-mRNA-1
chr1_2	exon	10940638	10940832	10940638	ID=Msa0055750-mRNA-1:exon:4171;Parent=Msa0055750-mRNA-1
chr1_2	exon	10940434	10940586	10940434	ID=Msa0055750-mRNA-1:exon:4170;Parent=Msa0055750-mRNA-1
chr1_2	exon	10940191	10940352	10940191	ID=Msa0055750-mRNA-1:exon:4169;Parent=Msa0055750-mRNA-1
chr1_2	exon	10939863	10940039	10939863	ID=Msa0055750-mRNA-1:exon:4168;Parent=Msa0055750-mRNA-1
chr1_2	exon	10939455	10939694	10939455	ID=Msa0055750-mRNA-1:exon:4167;Parent=Msa0055750-mRNA-1
chr1_2	exon	10939249	10939436	10939249	ID=Msa0055750-mRNA-1:exon:4166;Parent=Msa0055750-mRNA-1
chr1_2	exon	10939077	10939188	10939077	ID=Msa0055750-mRNA-1:exon:4165;Parent=Msa0055750-mRNA-1
chr1_2	exon	10938828	10939058	10938828	ID=Msa0055750-mRNA-1:exon:4164;Parent=Msa0055750-mRNA-1
chr1_2	exon	10938543	10938764	10938543	ID=Msa0055750-mRNA-1:exon:4163;Parent=Msa0055750-mRNA-1
chr1_2	exon	10933680	10933916	10933680	ID=Msa0055750-mRNA-1:exon:4162;Parent=Msa0055750-mRNA-1
chr1_2	exon	10928648	10928767	10928648	ID=Msa0055750-mRNA-1:exon:4161;Parent=Msa0055750-mRNA-1
chr1_2	exon	10928140	10928271	10928140	ID=Msa0055750-mRNA-1:exon:4160;Parent=Msa0055750-mRNA-1
chr1_2	exon	10926804	10928005	10926804	ID=Msa0055750-mRNA-1:exon:4159;Parent=Msa0055750-mRNA-1
chr1_2	CDS	10940839	10941070	10940839	ID=Msa0055750-mRNA-1:cds;Parent=Msa0055750-mRNA-1
chr1_2	CDS	10940638	10940832	10940638	ID=Msa0055750-mRNA-1:cds;Parent=Msa0055750-mRNA-1
chr1_2	CDS	10940434	10940586	10940434	ID=Msa0055750-mRNA-1:cds;Parent=Msa0055750-mRNA-1
chr1_2	CDS	10940191	10940352	10940191	ID=Msa0055750-mRNA-1:cds;Parent=Msa0055750-mRNA-1
chr1_2	CDS	10939863	10940039	10939863	ID=Msa0055750-mRNA-1:cds;Parent=Msa0055750-mRNA-1
chr1_2	CDS	10939455	10939694	10939455	ID=Msa0055750-mRNA-1:cds;Parent=Msa0055750-mRNA-1
chr1_2	CDS	10939249	10939436	10939249	ID=Msa0055750-mRNA-1:cds;Parent=Msa0055750-mRNA-1
chr1_2	CDS	10939077	10939188	10939077	ID=Msa0055750-mRNA-1:cds;Parent=Msa0055750-mRNA-1
chr1_2	CDS	10938828	10939058	10938828	ID=Msa0055750-mRNA-1:cds;Parent=Msa0055750-mRNA-1
chr1_2	CDS	10938543	10938764	10938543	ID=Msa0055750-mRNA-1:cds;Parent=Msa0055750-mRNA-1
chr1_2	CDS	10933680	10933916	10933680	ID=Msa0055750-mRNA-1:cds;Parent=Msa0055750-mRNA-1
chr1_2	CDS	10928648	10928767	10928648	ID=Msa0055750-mRNA-1:cds;Parent=Msa0055750-mRNA-1
chr1_2	CDS	10928140	10928271	10928140	ID=Msa0055750-mRNA-1:cds;Parent=Msa0055750-mRNA-1
chr1_2	CDS	10926804	10928005	10926804	ID=Msa0055750-mRNA-1:cds;Parent=Msa0055750-mRNA-1

Gene Sequence

>Msa0055750

ATGGTTGCACTATTATTGTTATTATTATCTTTCTCTCTTCTGCAAGTAGGAGCAATTGCATCAGCAGAATCAGCAGAAAGACAAGCACTGCCTGGCTGTGTATACAACAGTCCTTGTGGAAATGTAACAATTCCATATCCATTTGGCATAGGAATTTCAACACAAGATCAAGCACCATGTTACTTGGATCGTAAATTTGAGCTCACTTGTACTGAAGACTCCAAGTTAGTCCGTAAGAACTCCGAAATCCTTGACATAAAACCTGAAGGTCAATTTGTTGTGTTGATTCCAGTATCAAAGTATTGTAGTGCCAAAAATTACAAACCTGTCGCTGTTGGAGTCTCTAATTTCAGCATTTCAAGCAAGGAAAACCAGTTCATAACCGTTGGATGCGACAGTTATGGTTATTTTAATAGCATTTATGACGTGTTTGATAATGGAACCTGCTCCGGTATAGGTTGCTGCGAGGTTGATATTCCTCCTAGAATGAGGAACCTCTCTGTAGAAACTTCTTCCTTCAATACTTCGTTGGAACTACCTTCACTAAACTGCAGCTATTCCTTTGTTGCTAAGAAACACAGTGAGTTGCATGAAAACTGCAGTATTGCTTCTTCCAATAATAAAACCTATGCGTGCAAGAACAATACCAAATGCGATGACAGCGACACAGATTTTGGTTACCGATGTAGATGCAAAGAAGGTTATGCAGGAAATCCCTACCTGCATCCCCATGGCTGCACAGACATCGACGAATGTGAAACAAAATTACATGAATGCAAAACAAAAGCATATTGTCACAACACCGATGGGAGTCACACATGTTTGTGTCCAAAGGGGTATTCAGGAGATGGAAAAAAGGAAGGAGGGTGCCAACTCCTTGATAAAAATAAAAATTCCATGGTATTATCTATTGTCCTTGGAGTAGGAGTAACATTTATTCTTCTCTTTTTGGGGACATTCTCGTACTTAATGTATCATAAAAGGAAATTCATCAAATTGAGAGAGAAATTCTTTGAGCAGAATGGTGGTTTGATTTTGAAACAAAAACTCTCAACAAGAGAAGACTCCTCCTATCAAACAAGACAAGACTCCTCAACTCAAAGTGCTAAAATATTCACACAAGATCAGCTAAACAAGGCAACCAAAAACTTCGACGAGAGTGGTTTTGGTACCGTTTTCAAAGGATTTCTAGATGATAAAAGAATTGTTGCTATCAAAAAATCAAAAATAATAGATAGAAGCCAAATCGATCAATTCGTTAATGAGGTGGTTGTTCTCTCTCAAATAAATCATAGGAATGTTGTCAAACTCTTGGGATGTTGTTTAGAGACAGAAGTTCCTTTGTTGGTGAATAATGAAACATGGAAAACTCGTCTTAAGATAGCCGCAGAGGTAGCAGAAGCTTTGTCGTATCTACATTCATATGCCTCAACACCCATCATCCATAGAGATGTCAAGAATGGAACCAACACTGCCAAAGTGTCGGATTTTGGAGCTTCAAGATTGGTTCCAATCGATCAAACTGAAGTAGCCACAATGGTGCAAGGAACGATTGGATACTTAGATCCAGAGTATATGCAAACAAGCCAATTGACTGAAAAAAGTGATGTCTATAGCTTTGGAGTAGTTCTTGTGGAGTTGCTAACAGGGGAGAAGCCTTTTAGTTTTGGTAGGCCGGAAGAGAGAAGAAGTATGTGGAATGAAGAAAACAAACAAGAGATCAAGGAGGTGGCTATTCTTGCGGCGAAGTGTTTGAGACTTAGAGGGGAAGAAAGACCTAGCATGAAGGAAGTGGCTATGGAATTGGAGGGAATGAGGTTAATGAATAAGCACTCTTGGATCAATGATGACTCAAATGTAGAGAAGAGTCCGTACTTGCTTCATGAATCATCATCTAGCTTTTATGAACCTGCACTTTTGATAGAAGCAGTTCCATTTGCATTTGCATATGATCAACCTCAGCAAGTTCTACCTGGCTGCAATTACACTTGTGGAGGTGTAAAAGTTCCATATCCATTTGGCATAGGAAATTCAACAGCACAAAATCATACACCATGTTACATGAATTCCAAATTTTATCTAACTTGTGTAAACAACTCAAAACTAATCCGTGGTAACAATGTACAAGTCCTAGACATAAATCCCCAAGATAACTATGCCTGCAAGGAAAATAGTGAATGTGTTGATAAAGATTCAGATTATGACGGTTACAGATGCAAGTGTTTGGCAGGTTTTGAAGGAAATCCATACCTTCCCGGAGGCTGCAGAGCAAATTGTAATAACACTTTTGGGAATTACACTTGTTTCTGTCCGATCGGGCAACTCGGAAATGGAACAAGGTTAGGAGGGTGCAAAGTACCTGATGGAAATGCATATCAAATAGTACTACCAACTGTCCGTGGAGTAGGAGCAGTATTTATTGTTCTATTTGTGTGGATAATTTCCTACTTAATATACCAAAAAAGAAAACTTGTCAAATTGAAAGAGAAATTCTTTAGGCAGAATGGAGGTTCTATTTTGGAACAGAAACTTCGTCAAAGAAAAGACTCGTCTCAAATAGCTCACATTTTCAAAGAAAATGAACTTAAAAAAGCCACTAACAACTTTGATGAGAGCTTAATCATAGGTAAAGGAGGTTTTGGTACAGTTTTCAAAGGAGAACTTGATGATAATAGAATTGTTGCTATCAAAAAGTCCAAAACAATAGATGAAAGCCAAATTGAACAATTCATTAATGAAGTGGATGTTGTCTCTCAAATAAATCACAGGAATGTGGTCAAACTCTTAGGGTGTTGTCTAGAAACAGAAGTTCCTTTGTTGGTTTATGAGTTTGTTAGCAATGGTACTCTTTCTGAATTTATACACACACAAGGTAAGACAAACGATCTAACTTGGAAAACTCGTCTTAAGATAGCCGCAGAGGTAGCCGAAGCTCTGTCGTATCTACATTCATATGCCTCAATACCTATTATCCATAGAGATGTTAAGAGTGATAACATTCTCTTAGATGGAACCAACACTGCTAAAGTGTCGGATTTTGGAGCTTCAAGATTGGTTCCAATCGATCAAACTGAGGTAGCCACAATGGTGCAAGGAACTACTGGCTACTTAGATCCAGAGTATATGCAAACAAGCCAATTGACTGAAAAAAGTGATGTCTATAGCTTTGGAGTAGTTCTTGTGGAGCTTCTAACGGGGGAGAAACCTTTTTGTTTTGGTAGGTCGGAAGAGAGAAGAAGTCTTGCTATGCATTTTCTATCTTGCTTGAAAAAGGATAATGTGTTTGAAGTTATTCAAGATGGTATGTGGAATGAAGAAAACAAACAAGAGATCAAGGAGGTAGCTGTTCTTGCGGCGAAATGTTTGAGACTTAAAGGGGAGGAAAGACCAAGCATGAAGGAAGTGGCTATGGAATTGGAGGGAATGAGGTTAATGAATAAGCACTCTTGGATCAATGATGACTTAAATGTAGATGAGAGTCGGTACTTGCTTCATGAATCATCATCTAGCCTTTATGAACCTGGTGATAGTGGCAAACATGGAGATATTGGATACGACAGCCTGAAAGAACTTGAACTGGTAGTATTGGACGATGGAAGGTGA

Protein sequence

>Msa0055750

MVALLLLLLSFSLLQVGAIASAESAERQALPGCVYNSPCGNVTIPYPFGIGISTQDQAPCYLDRKFELTCTEDSKLVRKNSEILDIKPEGQFVVLIPVSKYCSAKNYKPVAVGVSNFSISSKENQFITVGCDSYGYFNSIYDVFDNGTCSGIGCCEVDIPPRMRNLSVETSSFNTSLELPSLNCSYSFVAKKHSELHENCSIASSNNKTYACKNNTKCDDSDTDFGYRCRCKEGYAGNPYLHPHGCTDIDECETKLHECKTKAYCHNTDGSHTCLCPKGYSGDGKKEGGCQLLDKNKNSMVLSIVLGVGVTFILLFLGTFSYLMYHKRKFIKLREKFFEQNGGLILKQKLSTREDSSYQTRQDSSTQSAKIFTQDQLNKATKNFDESGFGTVFKGFLDDKRIVAIKKSKIIDRSQIDQFVNEVVVLSQINHRNVVKLLGCCLETEVPLLVNNETWKTRLKIAAEVAEALSYLHSYASTPIIHRDVKNGTNTAKVSDFGASRLVPIDQTEVATMVQGTIGYLDPEYMQTSQLTEKSDVYSFGVVLVELLTGEKPFSFGRPEERRSMWNEENKQEIKEVAILAAKCLRLRGEERPSMKEVAMELEGMRLMNKHSWINDDSNVEKSPYLLHESSSSFYEPALLIEAVPFAFAYDQPQQVLPGCNYTCGGVKVPYPFGIGNSTAQNHTPCYMNSKFYLTCVNNSKLIRGNNVQVLDINPQDNYACKENSECVDKDSDYDGYRCKCLAGFEGNPYLPGGCRANCNNTFGNYTCFCPIGQLGNGTRLGGCKVPDGNAYQIVLPTVRGVGAVFIVLFVWIISYLIYQKRKLVKLKEKFFRQNGGSILEQKLRQRKDSSQIAHIFKENELKKATNNFDESLIIGKGGFGTVFKGELDDNRIVAIKKSKTIDESQIEQFINEVDVVSQINHRNVVKLLGCCLETEVPLLVYEFVSNGTLSEFIHTQGKTNDLTWKTRLKIAAEVAEALSYLHSYASIPIIHRDVKSDNILLDGTNTAKVSDFGASRLVPIDQTEVATMVQGTTGYLDPEYMQTSQLTEKSDVYSFGVVLVELLTGEKPFCFGRSEERRSLAMHFLSCLKKDNVFEVIQDGMWNEENKQEIKEVAVLAAKCLRLKGEERPSMKEVAMELEGMRLMNKHSWINDDLNVDESRYLLHESSSSLYEPGDSGKHGDIGYDSLKELELVVLDDGR*