AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1328290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1328290 MtrunA17_Chr4g0067471 95.098 102 5 0 1 102 1 102 7.12e-70 203
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1328290 AT4G15910.1 41.379 87 48 1 15 101 20 103 1.18e-15 67.0
Msa1328290 AT1G02820.1 41.096 73 33 2 23 95 25 87 4.56e-11 54.7

Find 59 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTCTGTAGTATCCGGTTTC+TGG 0.094835 8_4:-45910388 None:intergenic
ACAATACCATAACGGGATTT+TGG 0.099072 8_4:+45910512 Msa1328290:three_prime_UTR
TGCTCTCTGCCCTTCATGTT+AGG 0.220831 8_4:-45909213 None:intergenic
GCTCTCTGCCCTTCATGTTA+GGG 0.329683 8_4:-45909212 None:intergenic
GCTTGCAACTCCCCTCTATT+AGG 0.330302 8_4:-45910482 None:intergenic
TTCCCAGCTTTCCGTGGCCA+TGG 0.335601 8_4:-45910303 None:intergenic
TAGACATGGGCTTCTCTTCA+AGG 0.339449 8_4:-45910327 None:intergenic
GTCTGGCGCCCATGCCGAAC+AGG 0.356618 8_4:-45910365 None:intergenic
TACAATTACAATACCATAAC+GGG 0.368204 8_4:+45910505 Msa1328290:three_prime_UTR
CTACTTAAATAATAAAACTA+TGG 0.368314 8_4:+45909318 Msa1328290:exon
AGCCCATGGCCACGGAAAGC+TGG 0.388015 8_4:+45910300 Msa1328290:CDS
GTCTGTAGTATCCGGTTTCT+GGG 0.391156 8_4:-45910387 None:intergenic
TAGTATCCGGTTTCTGGGTC+TGG 0.400740 8_4:-45910382 None:intergenic
TGCTTCAGCTAGAGTTGGAT+TGG 0.428759 8_4:+45910270 Msa1328290:CDS
CTGACCGTTGAAGATGAAAC+AGG 0.428944 8_4:-45909387 None:intergenic
TCCCCTCTATTAGGATGATC+TGG 0.432852 8_4:-45910473 None:intergenic
CCCATGCCGAACAGGCCTCT+TGG 0.447286 8_4:-45910357 None:intergenic
TCCCAGCTTTCCGTGGCCAT+GGG 0.452186 8_4:-45910302 None:intergenic
TGGGCGCCAGACCCAGAAAC+CGG 0.464783 8_4:+45910376 Msa1328290:CDS
CTCTTGGCCATCTCTAGACA+TGG 0.474198 8_4:-45910341 None:intergenic
AGATGGCCAAGAGGCCTGTT+CGG 0.475265 8_4:+45910351 Msa1328290:CDS
ATACCAGATCATCCTAATAG+AGG 0.484143 8_4:+45910470 Msa1328290:exon
CTTCATGTTAGGGTTTGTAA+AGG 0.484539 8_4:-45909202 None:intergenic
GCCAAGAGGCCTGTTCGGCA+TGG 0.485879 8_4:+45910356 Msa1328290:CDS
CCAAGAGGCCTGTTCGGCAT+GGG 0.486297 8_4:+45910357 Msa1328290:CDS
AGAGCAATAGCTGGCTGCTG+CGG 0.488075 8_4:+45909230 Msa1328290:five_prime_UTR
AGCATCTGTCGGAGCTCCAC+CGG 0.490620 8_4:-45910436 None:intergenic
ATTGATGGGTCTGTAGTATC+CGG 0.491419 8_4:-45910395 None:intergenic
CACATAGGAAGATAGATTGA+TGG 0.503669 8_4:-45909168 None:intergenic
GCTTCAGCTAGAGTTGGATT+GGG 0.505486 8_4:+45910271 Msa1328290:CDS
TACCAGATCATCCTAATAGA+GGG 0.511858 8_4:+45910471 Msa1328290:exon
TCTGATGCTTCAGCTAGAGT+TGG 0.513043 8_4:+45910265 Msa1328290:CDS
GCCCATGGCCACGGAAAGCT+GGG 0.515616 8_4:+45910301 Msa1328290:CDS
CAAGGCTTCCCAGCTTTCCG+TGG 0.538111 8_4:-45910309 None:intergenic
TGCGTTTGAGTAGCATCTGT+CGG 0.543834 8_4:-45910447 None:intergenic
CTACAATTACAATACCATAA+CGG 0.554588 8_4:+45910504 Msa1328290:three_prime_UTR
GTTAGCTTGTGAGAGAGAGC+AGG 0.562013 8_4:-45909341 None:intergenic
TATTAGTGTGCTGCTGCGGA+AGG 0.563539 8_4:-45909291 None:intergenic
GAAGGGCAGAGAGCAATAGC+TGG 0.564242 8_4:+45909221 Msa1328290:five_prime_UTR
CTTTACAAACCCTAACATGA+AGG 0.564323 8_4:+45909203 Msa1328290:five_prime_UTR
AACTCTAGCTGAAGCATCAG+AGG 0.572077 8_4:-45910263 None:intergenic
GCATCTGTCGGAGCTCCACC+GGG 0.595147 8_4:-45910435 None:intergenic
ACATAGGAAGATAGATTGAT+GGG 0.596231 8_4:-45909167 None:intergenic
TCTTGGCCATCTCTAGACAT+GGG 0.599128 8_4:-45910340 None:intergenic
TAGTTATTAGTGTGCTGCTG+CGG 0.602809 8_4:-45909295 None:intergenic
GAGAAGCCCATGTCTAGAGA+TGG 0.605275 8_4:+45910334 Msa1328290:CDS
GGTAGTATAGCCCATGGCCA+CGG 0.610891 8_4:+45910292 Msa1328290:CDS
AGGAAGATAGATTGATGGGA+CGG 0.613017 8_4:-45909163 None:intergenic
TTTACAAACCCTAACATGAA+GGG 0.653000 8_4:+45909204 Msa1328290:five_prime_UTR
TGACCGTTGAAGATGAAACA+GGG 0.659345 8_4:-45909386 None:intergenic
ACCAGATCATCCTAATAGAG+GGG 0.663649 8_4:+45910472 Msa1328290:exon
GGAGAGATTGAGCAACAAGA+CGG 0.667162 8_4:-45909363 None:intergenic
GGATTGGGTAGTATAGCCCA+TGG 0.667307 8_4:+45910286 Msa1328290:CDS
TCTAGCTGAAGCATCAGAGG+CGG 0.672860 8_4:-45910260 None:intergenic
CACTCCTAAAATTGACCCGG+TGG 0.689227 8_4:+45910420 Msa1328290:CDS
TTACACTCCTAAAATTGACC+CGG 0.690275 8_4:+45910417 Msa1328290:CDS
CATGTCTAGAGATGGCCAAG+AGG 0.705730 8_4:+45910342 Msa1328290:CDS
ACCGTTGAAGATGAAACAGG+GGG 0.706818 8_4:-45909384 None:intergenic
GACCGTTGAAGATGAAACAG+GGG 0.712207 8_4:-45909385 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATATGTAAAATTTAGTT+TGG - chr8_4:45910007-45910026 None:intergenic 10.0%
!! TTTATATGTAAAATTTAGTT+TGG - chr8_4:45910007-45910026 None:intergenic 10.0%
!! CTACTTAAATAATAAAACTA+TGG + chr8_4:45909318-45909337 Msa1328290:exon 15.0%
!! ATAATATTATGAATCAAAGT+TGG - chr8_4:45909415-45909434 None:intergenic 15.0%
!!! ATCTTACACAAATTTAATTT+AGG + chr8_4:45909617-45909636 Msa1328290:intron 15.0%
!!! TATTTTAAAAGCCTTAAAAT+CGG + chr8_4:45909658-45909677 Msa1328290:intron 15.0%
!!! TTTACTTTTACAATTGTTTT+AGG + chr8_4:45909958-45909977 Msa1328290:intron 15.0%
!!! AAATTTTACATATAAACCAA+TGG + chr8_4:45910011-45910030 Msa1328290:intron 15.0%
!! CAAATTATACTTTAAACTAA+GGG + chr8_4:45910091-45910110 Msa1328290:intron 15.0%
!!! TGAATTTTTAAAACAATGTA+AGG + chr8_4:45910173-45910192 Msa1328290:intron 15.0%
!!! GAATTTTTAAAACAATGTAA+GGG + chr8_4:45910174-45910193 Msa1328290:intron 15.0%
!! CTACTTAAATAATAAAACTA+TGG + chr8_4:45909318-45909337 Msa1328290:exon 15.0%
!! ATAATATTATGAATCAAAGT+TGG - chr8_4:45909415-45909434 None:intergenic 15.0%
!!! ATCTTACACAAATTTAATTT+AGG + chr8_4:45909617-45909636 Msa1328290:intron 15.0%
!!! TATTTTAAAAGCCTTAAAAT+CGG + chr8_4:45909658-45909677 Msa1328290:intron 15.0%
!!! TTTACTTTTACAATTGTTTT+AGG + chr8_4:45909958-45909977 Msa1328290:intron 15.0%
!!! AAATTTTACATATAAACCAA+TGG + chr8_4:45910011-45910030 Msa1328290:intron 15.0%
!! CAAATTATACTTTAAACTAA+GGG + chr8_4:45910091-45910110 Msa1328290:intron 15.0%
!!! TGAATTTTTAAAACAATGTA+AGG + chr8_4:45910173-45910192 Msa1328290:intron 15.0%
!!! GAATTTTTAAAACAATGTAA+GGG + chr8_4:45910174-45910193 Msa1328290:intron 15.0%
!! TCTGTGATTATATATGAATT+AGG + chr8_4:45909461-45909480 Msa1328290:intron 20.0%
!! CTGTGATTATATATGAATTA+GGG + chr8_4:45909462-45909481 Msa1328290:intron 20.0%
!!! ATATGAACTTTAGTTTGTTT+TGG + chr8_4:45909548-45909567 Msa1328290:intron 20.0%
!! CAAATAATACTTACATAGTT+TGG - chr8_4:45909755-45909774 None:intergenic 20.0%
!! AACTATGTAAGTATTATTTG+CGG + chr8_4:45909755-45909774 Msa1328290:intron 20.0%
!!! CAAGATAGTTTTATTATGTT+TGG + chr8_4:45909882-45909901 Msa1328290:intron 20.0%
!! TCTATGTATAAAGTTTAGTT+TGG - chr8_4:45910057-45910076 None:intergenic 20.0%
!! CTAAACTTTATACATAGATT+AGG + chr8_4:45910059-45910078 Msa1328290:intron 20.0%
!!! CCTTAGTTTAAAGTATAATT+TGG - chr8_4:45910093-45910112 None:intergenic 20.0%
!! CCAAATTATACTTTAAACTA+AGG + chr8_4:45910090-45910109 Msa1328290:intron 20.0%
!! TAAATGTTCAAATAAATCTC+TGG - chr8_4:45910201-45910220 None:intergenic 20.0%
!!! TTTTTGAATGTTTCACTTTA+TGG + chr8_4:45910616-45910635 Msa1328290:three_prime_UTR 20.0%
!! TCTGTGATTATATATGAATT+AGG + chr8_4:45909461-45909480 Msa1328290:intron 20.0%
!! CTGTGATTATATATGAATTA+GGG + chr8_4:45909462-45909481 Msa1328290:intron 20.0%
!!! ATATGAACTTTAGTTTGTTT+TGG + chr8_4:45909548-45909567 Msa1328290:intron 20.0%
!! CAAATAATACTTACATAGTT+TGG - chr8_4:45909755-45909774 None:intergenic 20.0%
!! AACTATGTAAGTATTATTTG+CGG + chr8_4:45909755-45909774 Msa1328290:intron 20.0%
!!! CAAGATAGTTTTATTATGTT+TGG + chr8_4:45909882-45909901 Msa1328290:intron 20.0%
!! TCTATGTATAAAGTTTAGTT+TGG - chr8_4:45910057-45910076 None:intergenic 20.0%
!! CTAAACTTTATACATAGATT+AGG + chr8_4:45910059-45910078 Msa1328290:intron 20.0%
!!! CCTTAGTTTAAAGTATAATT+TGG - chr8_4:45910093-45910112 None:intergenic 20.0%
!! CCAAATTATACTTTAAACTA+AGG + chr8_4:45910090-45910109 Msa1328290:intron 20.0%
!! TAAATGTTCAAATAAATCTC+TGG - chr8_4:45910201-45910220 None:intergenic 20.0%
!!! TTTTTGAATGTTTCACTTTA+TGG + chr8_4:45910616-45910635 Msa1328290:three_prime_UTR 20.0%
! AATCAAACCTATAGTTGAAA+GGG - chr8_4:45909716-45909735 None:intergenic 25.0%
! AAATCAAACCTATAGTTGAA+AGG - chr8_4:45909717-45909736 None:intergenic 25.0%
! ATATAGTTAACCTCATAAGA+GGG - chr8_4:45909853-45909872 None:intergenic 25.0%
! TATATAGTTAACCTCATAAG+AGG - chr8_4:45909854-45909873 None:intergenic 25.0%
! TGGTTATTTAACAGATATTC+AGG + chr8_4:45909902-45909921 Msa1328290:intron 25.0%
! GGTTATTTAACAGATATTCA+GGG + chr8_4:45909903-45909922 Msa1328290:intron 25.0%
!!! ATTGTTTTAGGTTTAACTGT+AGG + chr8_4:45909970-45909989 Msa1328290:intron 25.0%
!!! TTGTTTTAGGTTTAACTGTA+GGG + chr8_4:45909971-45909990 Msa1328290:intron 25.0%
!!! AAAGTTTTAATTTGGTCCAT+TGG - chr8_4:45910030-45910049 None:intergenic 25.0%
!!! TTGGTCAGAAAGTTTTAATT+TGG - chr8_4:45910038-45910057 None:intergenic 25.0%
! TTATACATAGATTAGGATCT+AGG + chr8_4:45910066-45910085 Msa1328290:intron 25.0%
! CTATGGTAAATTGTTAAATC+TGG - chr8_4:45910138-45910157 None:intergenic 25.0%
!!! ATTTTAGGAGTGTAATTGAT+GGG - chr8_4:45910412-45910431 None:intergenic 25.0%
!!! AATTTTAGGAGTGTAATTGA+TGG - chr8_4:45910413-45910432 None:intergenic 25.0%
! CTACAATTACAATACCATAA+CGG + chr8_4:45910504-45910523 Msa1328290:three_prime_UTR 25.0%
! TACAATTACAATACCATAAC+GGG + chr8_4:45910505-45910524 Msa1328290:three_prime_UTR 25.0%
!! AACAAAATGTTTTGCTTTAG+AGG - chr8_4:45910587-45910606 None:intergenic 25.0%
! AATCAAACCTATAGTTGAAA+GGG - chr8_4:45909716-45909735 None:intergenic 25.0%
! AAATCAAACCTATAGTTGAA+AGG - chr8_4:45909717-45909736 None:intergenic 25.0%
! ATATAGTTAACCTCATAAGA+GGG - chr8_4:45909853-45909872 None:intergenic 25.0%
! TATATAGTTAACCTCATAAG+AGG - chr8_4:45909854-45909873 None:intergenic 25.0%
! TGGTTATTTAACAGATATTC+AGG + chr8_4:45909902-45909921 Msa1328290:intron 25.0%
! GGTTATTTAACAGATATTCA+GGG + chr8_4:45909903-45909922 Msa1328290:intron 25.0%
!!! ATTGTTTTAGGTTTAACTGT+AGG + chr8_4:45909970-45909989 Msa1328290:intron 25.0%
!!! TTGTTTTAGGTTTAACTGTA+GGG + chr8_4:45909971-45909990 Msa1328290:intron 25.0%
!!! AAAGTTTTAATTTGGTCCAT+TGG - chr8_4:45910030-45910049 None:intergenic 25.0%
!!! TTGGTCAGAAAGTTTTAATT+TGG - chr8_4:45910038-45910057 None:intergenic 25.0%
! TTATACATAGATTAGGATCT+AGG + chr8_4:45910066-45910085 Msa1328290:intron 25.0%
! CTATGGTAAATTGTTAAATC+TGG - chr8_4:45910138-45910157 None:intergenic 25.0%
!!! ATTTTAGGAGTGTAATTGAT+GGG - chr8_4:45910412-45910431 None:intergenic 25.0%
!!! AATTTTAGGAGTGTAATTGA+TGG - chr8_4:45910413-45910432 None:intergenic 25.0%
! CTACAATTACAATACCATAA+CGG + chr8_4:45910504-45910523 Msa1328290:three_prime_UTR 25.0%
! TACAATTACAATACCATAAC+GGG + chr8_4:45910505-45910524 Msa1328290:three_prime_UTR 25.0%
!! AACAAAATGTTTTGCTTTAG+AGG - chr8_4:45910587-45910606 None:intergenic 25.0%
ACATAGGAAGATAGATTGAT+GGG - chr8_4:45909170-45909189 None:intergenic 30.0%
! AAGGCTTTTATAATGCACAT+AGG - chr8_4:45909186-45909205 None:intergenic 30.0%
TTTACAAACCCTAACATGAA+GGG + chr8_4:45909204-45909223 Msa1328290:five_prime_UTR 30.0%
!!! TTTAGTTTGTTTTGGTGAAG+TGG + chr8_4:45909556-45909575 Msa1328290:intron 30.0%
!!! TTAGTTTGTTTTGGTGAAGT+GGG + chr8_4:45909557-45909576 Msa1328290:intron 30.0%
!! GACTAACTTAACCGATTTTA+AGG - chr8_4:45909672-45909691 None:intergenic 30.0%
! CAGTTTGATCATTTTCTCTA+TGG - chr8_4:45910155-45910174 None:intergenic 30.0%
!! TATCACTTATTTTTCCCTGT+AGG + chr8_4:45910224-45910243 Msa1328290:intron 30.0%
ACATAGGAAGATAGATTGAT+GGG - chr8_4:45909170-45909189 None:intergenic 30.0%
! AAGGCTTTTATAATGCACAT+AGG - chr8_4:45909186-45909205 None:intergenic 30.0%
TTTACAAACCCTAACATGAA+GGG + chr8_4:45909204-45909223 Msa1328290:five_prime_UTR 30.0%
!!! TTTAGTTTGTTTTGGTGAAG+TGG + chr8_4:45909556-45909575 Msa1328290:intron 30.0%
!!! TTAGTTTGTTTTGGTGAAGT+GGG + chr8_4:45909557-45909576 Msa1328290:intron 30.0%
!! GACTAACTTAACCGATTTTA+AGG - chr8_4:45909672-45909691 None:intergenic 30.0%
! CAGTTTGATCATTTTCTCTA+TGG - chr8_4:45910155-45910174 None:intergenic 30.0%
!! TATCACTTATTTTTCCCTGT+AGG + chr8_4:45910224-45910243 Msa1328290:intron 30.0%
CACATAGGAAGATAGATTGA+TGG - chr8_4:45909171-45909190 None:intergenic 35.0%
CTTCATGTTAGGGTTTGTAA+AGG - chr8_4:45909205-45909224 None:intergenic 35.0%
CTTTACAAACCCTAACATGA+AGG + chr8_4:45909203-45909222 Msa1328290:five_prime_UTR 35.0%
GTCTCTTAGCTTATCTATGT+TGG + chr8_4:45909689-45909708 Msa1328290:intron 35.0%
TACTCCTAACAAGTGTCTTA+GGG - chr8_4:45909795-45909814 None:intergenic 35.0%
TTACTCCTAACAAGTGTCTT+AGG - chr8_4:45909796-45909815 None:intergenic 35.0%
TAACAGATATTCAGGGAACT+TGG + chr8_4:45909910-45909929 Msa1328290:intron 35.0%
TTACACTCCTAAAATTGACC+CGG + chr8_4:45910417-45910436 Msa1328290:CDS 35.0%
ATACCAGATCATCCTAATAG+AGG + chr8_4:45910470-45910489 Msa1328290:exon 35.0%
TACCAGATCATCCTAATAGA+GGG + chr8_4:45910471-45910490 Msa1328290:exon 35.0%
!! ACAATACCATAACGGGATTT+TGG + chr8_4:45910512-45910531 Msa1328290:three_prime_UTR 35.0%
!! CAATACCATAACGGGATTTT+GGG + chr8_4:45910513-45910532 Msa1328290:three_prime_UTR 35.0%
AAAAACCCAAAATCCCGTTA+TGG - chr8_4:45910521-45910540 None:intergenic 35.0%
CACATAGGAAGATAGATTGA+TGG - chr8_4:45909171-45909190 None:intergenic 35.0%
CTTCATGTTAGGGTTTGTAA+AGG - chr8_4:45909205-45909224 None:intergenic 35.0%
CTTTACAAACCCTAACATGA+AGG + chr8_4:45909203-45909222 Msa1328290:five_prime_UTR 35.0%
GTCTCTTAGCTTATCTATGT+TGG + chr8_4:45909689-45909708 Msa1328290:intron 35.0%
TACTCCTAACAAGTGTCTTA+GGG - chr8_4:45909795-45909814 None:intergenic 35.0%
TTACTCCTAACAAGTGTCTT+AGG - chr8_4:45909796-45909815 None:intergenic 35.0%
TAACAGATATTCAGGGAACT+TGG + chr8_4:45909910-45909929 Msa1328290:intron 35.0%
TTACACTCCTAAAATTGACC+CGG + chr8_4:45910417-45910436 Msa1328290:CDS 35.0%
ATACCAGATCATCCTAATAG+AGG + chr8_4:45910470-45910489 Msa1328290:exon 35.0%
TACCAGATCATCCTAATAGA+GGG + chr8_4:45910471-45910490 Msa1328290:exon 35.0%
!! ACAATACCATAACGGGATTT+TGG + chr8_4:45910512-45910531 Msa1328290:three_prime_UTR 35.0%
!! CAATACCATAACGGGATTTT+GGG + chr8_4:45910513-45910532 Msa1328290:three_prime_UTR 35.0%
AAAAACCCAAAATCCCGTTA+TGG - chr8_4:45910521-45910540 None:intergenic 35.0%
AGGAAGATAGATTGATGGGA+CGG - chr8_4:45909166-45909185 None:intergenic 40.0%
! TAGTTATTAGTGTGCTGCTG+CGG - chr8_4:45909298-45909317 None:intergenic 40.0%
TGACCGTTGAAGATGAAACA+GGG - chr8_4:45909389-45909408 None:intergenic 40.0%
TGTTGGTCCCTTTCAACTAT+AGG + chr8_4:45909706-45909725 Msa1328290:intron 40.0%
ACTCCTAACAAGTGTCTTAG+GGG - chr8_4:45909794-45909813 None:intergenic 40.0%
CATCTGTTTACCCTCTTATG+AGG + chr8_4:45909840-45909859 Msa1328290:intron 40.0%
! ATTGATGGGTCTGTAGTATC+CGG - chr8_4:45910398-45910417 None:intergenic 40.0%
ACCAGATCATCCTAATAGAG+GGG + chr8_4:45910472-45910491 Msa1328290:exon 40.0%
AGGAAGATAGATTGATGGGA+CGG - chr8_4:45909166-45909185 None:intergenic 40.0%
! TAGTTATTAGTGTGCTGCTG+CGG - chr8_4:45909298-45909317 None:intergenic 40.0%
TGACCGTTGAAGATGAAACA+GGG - chr8_4:45909389-45909408 None:intergenic 40.0%
TGTTGGTCCCTTTCAACTAT+AGG + chr8_4:45909706-45909725 Msa1328290:intron 40.0%
ACTCCTAACAAGTGTCTTAG+GGG - chr8_4:45909794-45909813 None:intergenic 40.0%
CATCTGTTTACCCTCTTATG+AGG + chr8_4:45909840-45909859 Msa1328290:intron 40.0%
! ATTGATGGGTCTGTAGTATC+CGG - chr8_4:45910398-45910417 None:intergenic 40.0%
ACCAGATCATCCTAATAGAG+GGG + chr8_4:45910472-45910491 Msa1328290:exon 40.0%
GGAGAGATTGAGCAACAAGA+CGG - chr8_4:45909366-45909385 None:intergenic 45.0%
TCCCCCTGTTTCATCTTCAA+CGG + chr8_4:45909383-45909402 Msa1328290:CDS 45.0%
ACCGTTGAAGATGAAACAGG+GGG - chr8_4:45909387-45909406 None:intergenic 45.0%
GACCGTTGAAGATGAAACAG+GGG - chr8_4:45909388-45909407 None:intergenic 45.0%
CTGACCGTTGAAGATGAAAC+AGG - chr8_4:45909390-45909409 None:intergenic 45.0%
AGTCCCCTAAGACACTTGTT+AGG + chr8_4:45909788-45909807 Msa1328290:intron 45.0%
!! CTTATTTTTCCCTGTAGGCG+AGG + chr8_4:45910229-45910248 Msa1328290:intron 45.0%
! AACTCTAGCTGAAGCATCAG+AGG - chr8_4:45910266-45910285 None:intergenic 45.0%
TCTGATGCTTCAGCTAGAGT+TGG + chr8_4:45910265-45910284 Msa1328290:CDS 45.0%
TGCTTCAGCTAGAGTTGGAT+TGG + chr8_4:45910270-45910289 Msa1328290:CDS 45.0%
GCTTCAGCTAGAGTTGGATT+GGG + chr8_4:45910271-45910290 Msa1328290:CDS 45.0%
TAGACATGGGCTTCTCTTCA+AGG - chr8_4:45910330-45910349 None:intergenic 45.0%
TCTTGGCCATCTCTAGACAT+GGG - chr8_4:45910343-45910362 None:intergenic 45.0%
GTCTGTAGTATCCGGTTTCT+GGG - chr8_4:45910390-45910409 None:intergenic 45.0%
TGCGTTTGAGTAGCATCTGT+CGG - chr8_4:45910450-45910469 None:intergenic 45.0%
TCCCCTCTATTAGGATGATC+TGG - chr8_4:45910476-45910495 None:intergenic 45.0%
!!! GGGATTTTGGGTTTTTCTGC+TGG + chr8_4:45910525-45910544 Msa1328290:three_prime_UTR 45.0%
GGAGAGATTGAGCAACAAGA+CGG - chr8_4:45909366-45909385 None:intergenic 45.0%
TCCCCCTGTTTCATCTTCAA+CGG + chr8_4:45909383-45909402 Msa1328290:CDS 45.0%
ACCGTTGAAGATGAAACAGG+GGG - chr8_4:45909387-45909406 None:intergenic 45.0%
GACCGTTGAAGATGAAACAG+GGG - chr8_4:45909388-45909407 None:intergenic 45.0%
CTGACCGTTGAAGATGAAAC+AGG - chr8_4:45909390-45909409 None:intergenic 45.0%
AGTCCCCTAAGACACTTGTT+AGG + chr8_4:45909788-45909807 Msa1328290:intron 45.0%
!! CTTATTTTTCCCTGTAGGCG+AGG + chr8_4:45910229-45910248 Msa1328290:intron 45.0%
! AACTCTAGCTGAAGCATCAG+AGG - chr8_4:45910266-45910285 None:intergenic 45.0%
TCTGATGCTTCAGCTAGAGT+TGG + chr8_4:45910265-45910284 Msa1328290:CDS 45.0%
TGCTTCAGCTAGAGTTGGAT+TGG + chr8_4:45910270-45910289 Msa1328290:CDS 45.0%
GCTTCAGCTAGAGTTGGATT+GGG + chr8_4:45910271-45910290 Msa1328290:CDS 45.0%
TAGACATGGGCTTCTCTTCA+AGG - chr8_4:45910330-45910349 None:intergenic 45.0%
TCTTGGCCATCTCTAGACAT+GGG - chr8_4:45910343-45910362 None:intergenic 45.0%
GTCTGTAGTATCCGGTTTCT+GGG - chr8_4:45910390-45910409 None:intergenic 45.0%
TGCGTTTGAGTAGCATCTGT+CGG - chr8_4:45910450-45910469 None:intergenic 45.0%
TCCCCTCTATTAGGATGATC+TGG - chr8_4:45910476-45910495 None:intergenic 45.0%
!!! GGGATTTTGGGTTTTTCTGC+TGG + chr8_4:45910525-45910544 Msa1328290:three_prime_UTR 45.0%
GCTCTCTGCCCTTCATGTTA+GGG - chr8_4:45909215-45909234 None:intergenic 50.0%
TGCTCTCTGCCCTTCATGTT+AGG - chr8_4:45909216-45909235 None:intergenic 50.0%
! TATTAGTGTGCTGCTGCGGA+AGG - chr8_4:45909294-45909313 None:intergenic 50.0%
!! GTTAGCTTGTGAGAGAGAGC+AGG - chr8_4:45909344-45909363 None:intergenic 50.0%
! TCTAGCTGAAGCATCAGAGG+CGG - chr8_4:45910263-45910282 None:intergenic 50.0%
GGATTGGGTAGTATAGCCCA+TGG + chr8_4:45910286-45910305 Msa1328290:CDS 50.0%
GAGAAGCCCATGTCTAGAGA+TGG + chr8_4:45910334-45910353 Msa1328290:CDS 50.0%
CTCTTGGCCATCTCTAGACA+TGG - chr8_4:45910344-45910363 None:intergenic 50.0%
CATGTCTAGAGATGGCCAAG+AGG + chr8_4:45910342-45910361 Msa1328290:CDS 50.0%
! TAGTATCCGGTTTCTGGGTC+TGG - chr8_4:45910385-45910404 None:intergenic 50.0%
GGTCTGTAGTATCCGGTTTC+TGG - chr8_4:45910391-45910410 None:intergenic 50.0%
CACTCCTAAAATTGACCCGG+TGG + chr8_4:45910420-45910439 Msa1328290:CDS 50.0%
! AGCTCCACCGGGTCAATTTT+AGG - chr8_4:45910427-45910446 None:intergenic 50.0%
GCTTGCAACTCCCCTCTATT+AGG - chr8_4:45910485-45910504 None:intergenic 50.0%
GCTCTCTGCCCTTCATGTTA+GGG - chr8_4:45909215-45909234 None:intergenic 50.0%
TGCTCTCTGCCCTTCATGTT+AGG - chr8_4:45909216-45909235 None:intergenic 50.0%
! TATTAGTGTGCTGCTGCGGA+AGG - chr8_4:45909294-45909313 None:intergenic 50.0%
!! GTTAGCTTGTGAGAGAGAGC+AGG - chr8_4:45909344-45909363 None:intergenic 50.0%
! TCTAGCTGAAGCATCAGAGG+CGG - chr8_4:45910263-45910282 None:intergenic 50.0%
GGATTGGGTAGTATAGCCCA+TGG + chr8_4:45910286-45910305 Msa1328290:CDS 50.0%
GAGAAGCCCATGTCTAGAGA+TGG + chr8_4:45910334-45910353 Msa1328290:CDS 50.0%
CTCTTGGCCATCTCTAGACA+TGG - chr8_4:45910344-45910363 None:intergenic 50.0%
CATGTCTAGAGATGGCCAAG+AGG + chr8_4:45910342-45910361 Msa1328290:CDS 50.0%
! TAGTATCCGGTTTCTGGGTC+TGG - chr8_4:45910385-45910404 None:intergenic 50.0%
GGTCTGTAGTATCCGGTTTC+TGG - chr8_4:45910391-45910410 None:intergenic 50.0%
CACTCCTAAAATTGACCCGG+TGG + chr8_4:45910420-45910439 Msa1328290:CDS 50.0%
! AGCTCCACCGGGTCAATTTT+AGG - chr8_4:45910427-45910446 None:intergenic 50.0%
GCTTGCAACTCCCCTCTATT+AGG - chr8_4:45910485-45910504 None:intergenic 50.0%
GAAGGGCAGAGAGCAATAGC+TGG + chr8_4:45909221-45909240 Msa1328290:five_prime_UTR 55.0%
AGAGCAATAGCTGGCTGCTG+CGG + chr8_4:45909230-45909249 Msa1328290:five_prime_UTR 55.0%
GCTGCAAAACCTCGCCTACA+GGG - chr8_4:45910241-45910260 None:intergenic 55.0%
GGTAGTATAGCCCATGGCCA+CGG + chr8_4:45910292-45910311 Msa1328290:CDS 55.0%
AGATGGCCAAGAGGCCTGTT+CGG + chr8_4:45910351-45910370 Msa1328290:CDS 55.0%
GAAGGGCAGAGAGCAATAGC+TGG + chr8_4:45909221-45909240 Msa1328290:five_prime_UTR 55.0%
AGAGCAATAGCTGGCTGCTG+CGG + chr8_4:45909230-45909249 Msa1328290:five_prime_UTR 55.0%
GCTGCAAAACCTCGCCTACA+GGG - chr8_4:45910241-45910260 None:intergenic 55.0%
GGTAGTATAGCCCATGGCCA+CGG + chr8_4:45910292-45910311 Msa1328290:CDS 55.0%
AGATGGCCAAGAGGCCTGTT+CGG + chr8_4:45910351-45910370 Msa1328290:CDS 55.0%
GGCTGCAAAACCTCGCCTAC+AGG - chr8_4:45910242-45910261 None:intergenic 60.0%
TCCCAGCTTTCCGTGGCCAT+GGG - chr8_4:45910305-45910324 None:intergenic 60.0%
TTCCCAGCTTTCCGTGGCCA+TGG - chr8_4:45910306-45910325 None:intergenic 60.0%
CAAGGCTTCCCAGCTTTCCG+TGG - chr8_4:45910312-45910331 None:intergenic 60.0%
CCAAGAGGCCTGTTCGGCAT+GGG + chr8_4:45910357-45910376 Msa1328290:CDS 60.0%
AGCATCTGTCGGAGCTCCAC+CGG - chr8_4:45910439-45910458 None:intergenic 60.0%
GGCTGCAAAACCTCGCCTAC+AGG - chr8_4:45910242-45910261 None:intergenic 60.0%
TCCCAGCTTTCCGTGGCCAT+GGG - chr8_4:45910305-45910324 None:intergenic 60.0%
TTCCCAGCTTTCCGTGGCCA+TGG - chr8_4:45910306-45910325 None:intergenic 60.0%
CAAGGCTTCCCAGCTTTCCG+TGG - chr8_4:45910312-45910331 None:intergenic 60.0%
CCAAGAGGCCTGTTCGGCAT+GGG + chr8_4:45910357-45910376 Msa1328290:CDS 60.0%
AGCATCTGTCGGAGCTCCAC+CGG - chr8_4:45910439-45910458 None:intergenic 60.0%
AGCCCATGGCCACGGAAAGC+TGG + chr8_4:45910300-45910319 Msa1328290:CDS 65.0%
GCCCATGGCCACGGAAAGCT+GGG + chr8_4:45910301-45910320 Msa1328290:CDS 65.0%
GCCAAGAGGCCTGTTCGGCA+TGG + chr8_4:45910356-45910375 Msa1328290:CDS 65.0%
CCCATGCCGAACAGGCCTCT+TGG - chr8_4:45910360-45910379 None:intergenic 65.0%
TGGGCGCCAGACCCAGAAAC+CGG + chr8_4:45910376-45910395 Msa1328290:CDS 65.0%
GCATCTGTCGGAGCTCCACC+GGG - chr8_4:45910438-45910457 None:intergenic 65.0%
AGCCCATGGCCACGGAAAGC+TGG + chr8_4:45910300-45910319 Msa1328290:CDS 65.0%
GCCCATGGCCACGGAAAGCT+GGG + chr8_4:45910301-45910320 Msa1328290:CDS 65.0%
GCCAAGAGGCCTGTTCGGCA+TGG + chr8_4:45910356-45910375 Msa1328290:CDS 65.0%
CCCATGCCGAACAGGCCTCT+TGG - chr8_4:45910360-45910379 None:intergenic 65.0%
TGGGCGCCAGACCCAGAAAC+CGG + chr8_4:45910376-45910395 Msa1328290:CDS 65.0%
GCATCTGTCGGAGCTCCACC+GGG - chr8_4:45910438-45910457 None:intergenic 65.0%
GTCTGGCGCCCATGCCGAAC+AGG - chr8_4:45910368-45910387 None:intergenic 70.0%
GTCTGGCGCCCATGCCGAAC+AGG - chr8_4:45910368-45910387 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 45909150 45910673 45909150 ID=Msa1328290;Name=Msa1328290
chr8_4 mRNA 45909150 45910673 45909150 ID=Msa1328290-mRNA-1;Parent=Msa1328290;Name=Msa1328290-mRNA-1;_AED=0.02;_eAED=0.02;_QI=187|1|1|1|1|1|2|187|102
chr8_4 exon 45909150 45909404 45909150 ID=Msa1328290-mRNA-1:exon:12940;Parent=Msa1328290-mRNA-1
chr8_4 exon 45910246 45910673 45910246 ID=Msa1328290-mRNA-1:exon:12941;Parent=Msa1328290-mRNA-1
chr8_4 five_prime_UTR 45909150 45909336 45909150 ID=Msa1328290-mRNA-1:five_prime_utr;Parent=Msa1328290-mRNA-1
chr8_4 CDS 45909337 45909404 45909337 ID=Msa1328290-mRNA-1:cds;Parent=Msa1328290-mRNA-1
chr8_4 CDS 45910246 45910486 45910246 ID=Msa1328290-mRNA-1:cds;Parent=Msa1328290-mRNA-1
chr8_4 three_prime_UTR 45910487 45910673 45910487 ID=Msa1328290-mRNA-1:three_prime_utr;Parent=Msa1328290-mRNA-1
Gene Sequence

>Msa1328290

ATGGCCTGCTCTCTCTCACAAGCTAACCGTCTTGTTGCTCAATCTCTCCCCCTGTTTCATCTTCAACGGCGAGGTTTTGCAGCCGCCTCTGATGCTTCAGCTAGAGTTGGATTGGGTAGTATAGCCCATGGCCACGGAAAGCTGGGAAGCCTTGAAGAGAAGCCCATGTCTAGAGATGGCCAAGAGGCCTGTTCGGCATGGGCGCCAGACCCAGAAACCGGATACTACAGACCCATCAATTACACTCCTAAAATTGACCCGGTGGAGCTCCGACAGATGCTACTCAAACGCAATACCAGATCATCCTAA
Protein sequence

>Msa1328290

MACSLSQANRLVAQSLPLFHLQRRGFAAASDASARVGLGSIAHGHGKLGSLEEKPMSRDGQEACSAWAPDPETGYYRPINYTPKIDPVELRQMLLKRNTRSS*