AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0589690

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0589690 MtrunA17_Chr4g0006991 96.487 427 15 0 1 427 1 427 0.0 857
Msa0589690 MtrunA17_Chr7g0242701 77.049 427 98 0 1 427 1 427 0.0 668
Msa0589690 MtrunA17_Chr5g0437601 43.021 437 219 6 1 427 8 424 1.73e-130 382
Msa0589690 MtrunA17_Chr8g0360521 45.036 413 207 6 16 427 16 409 6.72e-129 378
Msa0589690 MtrunA17_Chr3g0113671 48.555 346 176 2 84 427 104 449 6.72e-125 369
Msa0589690 MtrunA17_Chr3g0114101 42.032 433 236 6 2 427 6 430 4.98e-121 358
Msa0589690 MtrunA17_Chr5g0437291 46.857 350 181 3 83 427 162 511 6.44e-121 361
Msa0589690 MtrunA17_Chr4g0005481 43.511 393 203 3 35 427 73 446 1.53e-114 342
Msa0589690 MtrunA17_Chr4g0005491 46.000 350 179 6 85 427 61 407 3.31e-112 335
Msa0589690 MtrunA17_Chr7g0243301 44.699 349 185 5 85 427 63 409 6.73e-109 327
Msa0589690 MtrunA17_Chr1g0210771 41.499 347 179 8 84 427 57 382 4.69e-90 278
Msa0589690 MtrunA17_Chr3g0105301 39.595 346 193 6 86 427 43 376 8.60e-89 274
Msa0589690 MtrunA17_Chr1g0171171 38.953 344 191 7 84 427 51 375 1.05e-84 263
Msa0589690 MtrunA17_Chr5g0430371 37.960 353 197 8 86 427 58 399 5.18e-84 263
Msa0589690 MtrunA17_Chr7g0269991 38.682 349 189 9 86 427 137 467 3.96e-82 260
Msa0589690 MtrunA17_Chr3g0097891 36.888 347 197 6 81 427 26 350 8.03e-82 255
Msa0589690 MtrunA17_Chr4g0068111 36.236 356 205 8 82 427 110 453 4.34e-81 257
Msa0589690 MtrunA17_Chr5g0413271 37.673 361 210 8 68 427 130 476 5.15e-81 258
Msa0589690 MtrunA17_Chr4g0071391 37.322 351 202 7 84 427 107 446 1.41e-80 255
Msa0589690 MtrunA17_Chr7g0258851 38.462 351 198 9 85 427 247 587 6.38e-79 255
Msa0589690 MtrunA17_Chr1g0175671 38.177 351 199 9 85 427 169 509 1.16e-78 253
Msa0589690 MtrunA17_Chr5g0413851 37.427 342 190 8 86 427 42 359 2.10e-75 239
Msa0589690 MtrunA17_Chr2g0283581 37.101 345 186 9 86 427 42 358 3.46e-75 238
Msa0589690 MtrunA17_Chr2g0287901 37.293 362 200 9 79 427 90 437 4.77e-75 241
Msa0589690 MtrunA17_Chr1g0213281 36.648 352 202 10 86 427 175 515 8.17e-75 243
Msa0589690 MtrunA17_Chr4g0041011 35.088 342 197 7 86 427 47 363 9.98e-75 238
Msa0589690 MtrunA17_Chr4g0045941 35.673 342 196 7 86 427 33 350 2.40e-74 236
Msa0589690 MtrunA17_Chr2g0314801 34.783 345 202 7 84 427 31 353 2.37e-73 233
Msa0589690 MtrunA17_Chr3g0085071 34.483 348 196 8 84 427 44 363 2.83e-73 234
Msa0589690 MtrunA17_Chr4g0045951 36.550 342 193 8 86 427 45 362 3.48e-73 233
Msa0589690 MtrunA17_Chr2g0325481 35.277 343 197 8 85 427 49 366 1.30e-72 232
Msa0589690 MtrunA17_Chr3g0085081 34.770 348 195 10 84 427 29 348 2.79e-72 231
Msa0589690 MtrunA17_Chr3g0091031 34.483 348 196 10 84 427 29 348 3.69e-71 228
Msa0589690 MtrunA17_Chr2g0314791 35.714 350 188 9 84 427 27 345 2.13e-70 226
Msa0589690 MtrunA17_Chr3g0126021 32.319 427 248 10 14 427 61 459 5.31e-70 228
Msa0589690 MtrunA17_Chr8g0368461 35.262 363 206 8 84 427 176 528 1.20e-69 229
Msa0589690 MtrunA17_Chr7g0259711 34.174 357 209 7 84 427 89 432 8.53e-69 225
Msa0589690 MtrunA17_Chr8g0384151 31.818 352 209 7 84 427 88 416 1.04e-63 211
Msa0589690 MtrunA17_Chr1g0209921 37.288 236 136 6 84 317 42 267 6.16e-51 172
Msa0589690 MtrunA17_Chr1g0178081 32.425 367 147 14 84 427 53 341 3.05e-49 171
Msa0589690 MtrunA17_Chr5g0414051 30.189 371 227 13 75 427 64 420 3.18e-43 157
Msa0589690 MtrunA17_Chr8g0391481 30.137 365 217 14 84 427 25 372 5.70e-42 152
Msa0589690 MtrunA17_Chr5g0414091 30.028 363 221 14 84 427 83 431 5.71e-42 153
Msa0589690 MtrunA17_Chr3g0140371 29.972 357 221 13 86 427 18 360 1.58e-39 145
Msa0589690 MtrunA17_Chr7g0226991 29.330 358 225 11 86 427 57 402 5.87e-39 144
Msa0589690 MtrunA17_Chr6g0456691 28.704 432 265 15 12 427 15 419 2.39e-38 143
Msa0589690 MtrunA17_Chr5g0414061 28.532 361 225 11 85 427 93 438 2.91e-38 143
Msa0589690 MtrunA17_Chr8g0347321 26.636 428 272 14 28 427 26 439 3.15e-38 143
Msa0589690 MtrunA17_Chr8g0387921 25.000 428 279 13 19 427 15 419 1.03e-37 141
Msa0589690 MtrunA17_Chr2g0283851 29.459 370 207 15 87 427 56 400 3.36e-37 140
Msa0589690 MtrunA17_Chr4g0070341 28.505 428 253 16 31 427 66 471 2.20e-36 139
Msa0589690 MtrunA17_Chr5g0414041 28.415 366 229 12 81 427 82 433 7.44e-36 137
Msa0589690 MtrunA17_Chr6g0456681 30.447 358 220 12 86 427 64 408 1.16e-35 136
Msa0589690 MtrunA17_Chr6g0476041 25.562 356 243 9 86 427 167 514 1.38e-35 137
Msa0589690 MtrunA17_Chr8g0384421 27.624 362 224 15 84 427 131 472 5.17e-35 135
Msa0589690 MtrunA17_Chr6g0451321 26.761 355 234 10 86 427 116 457 5.23e-34 132
Msa0589690 MtrunA17_Chr4g0049421 27.273 363 225 12 86 427 48 392 2.47e-33 129
Msa0589690 MtrunA17_Chr1g0188191 24.791 359 242 12 86 427 234 581 4.60e-32 128
Msa0589690 MtrunA17_Chr6g0455901 27.198 364 229 12 84 427 69 416 1.05e-28 116
Msa0589690 MtrunA17_Chr7g0221271 30.332 211 136 4 5 213 4 205 2.88e-23 97.1
Msa0589690 MtrunA17_Chr7g0221291 33.333 180 101 6 5 178 4 170 1.62e-22 94.0
Msa0589690 MtrunA17_Chr3g0085101 39.286 112 60 2 279 387 1 107 1.64e-19 84.0
Msa0589690 MtrunA17_Chr2g0287931 38.136 118 66 1 146 256 1 118 2.51e-18 80.9
Msa0589690 MtrunA17_Chr5g0429191 55.319 47 18 1 374 420 2 45 7.48e-12 60.1
Msa0589690 MtrunA17_Chr1g0209931 35.714 98 47 3 330 427 11 92 2.05e-11 60.5
Msa0589690 MtrunA17_Chr7g0221281 26.337 243 150 9 200 427 8 236 3.84e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0589690 AT5G01360.1 65.882 425 135 4 11 427 12 434 0.0 595
Msa0589690 AT5G01360.2 72.316 354 97 1 75 427 25 378 0.0 572
Msa0589690 AT2G40150.1 47.922 361 170 5 80 427 65 420 1.41e-126 373
Msa0589690 AT3G55990.1 46.594 367 192 3 63 427 119 483 4.61e-126 374
Msa0589690 AT1G73140.1 48.725 353 173 3 76 427 60 405 1.38e-125 370
Msa0589690 AT2G40320.1 41.505 412 229 3 17 427 22 422 9.99e-123 363
Msa0589690 AT3G11030.1 46.398 347 183 3 83 427 103 448 5.50e-119 354
Msa0589690 AT2G40160.2 44.628 363 185 7 76 427 66 423 6.58e-113 338
Msa0589690 AT2G40160.1 44.847 359 182 7 76 423 66 419 8.03e-113 338
Msa0589690 AT5G01620.2 44.828 348 186 5 82 427 106 449 5.04e-109 329
Msa0589690 AT5G01620.1 44.828 348 186 5 82 427 106 449 5.04e-109 329
Msa0589690 AT5G01620.3 44.828 348 186 5 82 427 114 457 8.54e-109 328
Msa0589690 AT2G38320.1 39.202 426 223 9 9 427 8 404 1.79e-104 316
Msa0589690 AT2G38320.2 43.034 323 176 5 111 427 1 321 1.49e-92 282
Msa0589690 AT3G62390.1 39.601 351 190 9 84 427 136 471 3.08e-88 276
Msa0589690 AT5G58600.1 38.701 354 200 8 79 427 59 400 3.31e-87 271
Msa0589690 AT5G20590.1 39.542 349 194 6 86 427 141 479 3.55e-86 271
Msa0589690 AT2G30900.1 39.024 369 195 12 63 427 23 365 1.07e-85 266
Msa0589690 AT1G78710.1 37.901 343 190 8 85 427 37 356 4.48e-84 262
Msa0589690 AT3G54260.1 37.971 345 190 7 86 427 51 374 2.07e-83 261
Msa0589690 AT5G06700.1 39.714 350 193 8 86 427 255 594 8.13e-83 266
Msa0589690 AT3G12060.1 36.554 383 213 8 52 427 176 535 2.13e-81 261
Msa0589690 AT2G34070.1 36.152 343 194 7 86 427 65 383 4.49e-80 252
Msa0589690 AT2G31110.2 36.812 345 190 9 85 427 44 362 8.32e-80 251
Msa0589690 AT5G49340.1 37.853 354 196 10 86 427 100 441 8.36e-80 254
Msa0589690 AT1G29050.1 35.673 342 196 6 86 427 61 378 4.65e-77 244
Msa0589690 AT2G30010.1 37.778 360 201 9 79 427 49 396 1.39e-76 244
Msa0589690 AT2G42570.1 34.571 350 205 8 78 427 39 364 1.66e-76 243
Msa0589690 AT2G30010.2 37.778 360 201 9 79 427 92 439 2.25e-76 244
Msa0589690 AT2G40160.3 44.841 252 125 5 76 318 66 312 7.78e-75 236
Msa0589690 AT1G48880.1 35.227 352 193 8 85 427 114 439 1.10e-72 235
Msa0589690 AT1G60790.1 34.060 367 223 9 71 427 175 532 4.68e-71 233
Msa0589690 AT3G14850.2 35.484 341 199 7 84 424 32 351 8.27e-71 228
Msa0589690 AT3G06080.2 36.134 357 199 10 86 427 103 445 1.80e-70 230
Msa0589690 AT5G19160.1 34.540 359 205 9 86 427 99 444 7.74e-67 220
Msa0589690 AT5G06230.2 34.417 369 194 11 84 427 19 364 4.94e-66 216
Msa0589690 AT5G06230.1 34.417 369 194 11 84 427 60 405 1.03e-65 216
Msa0589690 AT3G62390.2 39.918 243 135 5 84 319 136 374 2.86e-60 202
Msa0589690 AT1G78710.2 32.653 343 155 9 85 427 30 296 1.97e-59 196
Msa0589690 AT5G58600.2 39.004 241 138 4 79 318 59 291 3.44e-57 190
Msa0589690 AT2G30900.2 38.697 261 144 8 63 320 23 270 7.15e-57 189
Msa0589690 AT3G11570.1 30.023 433 245 12 12 427 28 419 1.45e-56 192
Msa0589690 AT5G20590.2 39.167 240 137 3 86 318 141 378 3.55e-56 190
Msa0589690 AT2G31110.3 37.445 227 129 5 85 309 44 259 6.25e-54 181
Msa0589690 AT2G34070.2 33.824 272 156 6 157 427 1 249 1.71e-52 176
Msa0589690 AT5G19160.2 35.938 256 153 4 86 334 99 350 2.49e-49 171
Msa0589690 AT3G06080.1 37.190 242 139 5 86 318 103 340 7.46e-49 170
Msa0589690 AT3G14850.1 33.582 268 158 6 157 424 1 248 1.06e-46 161
Msa0589690 AT5G06230.3 34.118 255 139 7 84 318 60 305 4.49e-44 156
Msa0589690 AT5G64020.1 29.570 372 212 15 86 427 57 408 4.57e-42 153
Msa0589690 AT2G31110.1 35.470 234 124 8 196 427 6 214 1.14e-41 147
Msa0589690 AT1G70230.1 28.852 357 223 11 82 427 75 411 3.18e-39 146
Msa0589690 AT1G01430.1 27.577 359 235 12 85 427 98 447 1.55e-38 145
Msa0589690 AT5G64470.3 27.171 357 234 13 83 427 52 394 1.93e-38 143
Msa0589690 AT5G64470.2 26.870 361 236 12 83 427 52 400 6.56e-38 142
Msa0589690 AT5G15890.1 27.595 395 235 12 54 427 158 522 4.22e-37 142
Msa0589690 AT4G23790.1 26.437 435 274 15 13 427 19 427 9.64e-37 139
Msa0589690 AT2G14530.1 27.624 362 225 10 86 427 62 406 9.94e-37 139
Msa0589690 AT2G37720.1 28.226 372 205 15 86 427 143 482 3.89e-35 136
Msa0589690 AT5G20680.6 26.904 394 246 14 55 427 85 457 4.66e-35 135
Msa0589690 AT4G01080.1 26.912 353 226 11 85 422 90 425 8.87e-35 134
Msa0589690 AT5G20680.2 26.904 394 246 14 55 427 161 533 9.15e-35 135
Msa0589690 AT5G20680.4 26.904 394 246 15 55 427 179 551 9.61e-35 135
Msa0589690 AT5G20680.1 26.904 394 246 15 55 427 179 551 9.61e-35 135
Msa0589690 AT5G20680.3 26.904 394 246 15 55 427 179 551 9.61e-35 135
Msa0589690 AT5G20680.5 26.904 394 246 15 55 427 179 551 9.61e-35 135
Msa0589690 AT3G02440.1 27.577 359 226 12 86 427 127 468 1.09e-34 134
Msa0589690 AT3G02440.2 27.577 359 226 12 86 427 135 476 1.28e-34 134
Msa0589690 AT4G25360.1 26.873 387 257 10 59 427 146 524 4.15e-34 134
Msa0589690 AT4G25360.2 26.873 387 257 10 59 427 146 524 4.15e-34 134
Msa0589690 AT5G51640.1 26.087 391 257 13 56 427 111 488 1.92e-33 131
Msa0589690 AT4G11090.1 26.260 377 225 15 80 427 73 425 9.45e-32 125
Msa0589690 AT3G28150.1 27.348 362 229 13 82 427 67 410 3.26e-29 118
Msa0589690 AT5G15900.1 27.248 367 222 12 84 427 65 409 9.01e-29 117
Msa0589690 AT5G15900.2 27.248 367 222 12 84 427 84 428 1.16e-28 117
Msa0589690 AT5G64470.4 26.838 272 181 11 83 343 52 316 8.84e-22 95.9
Msa0589690 AT5G64470.1 26.619 278 187 11 83 349 52 323 1.21e-21 95.5

Find 81 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGATTTGATGTGCTTTCT+AGG 0.201030 4_2:-12873825 None:intergenic
TCCTCAGTTTATAGTGAAAT+TGG 0.219607 4_2:+12876556 Msa0589690:CDS
TCAGAAACAATGAAGCTTCT+TGG 0.223944 4_2:+12873681 Msa0589690:CDS
TTCATTGATTTCTTCTTTGC+AGG 0.235261 4_2:-12874841 None:intergenic
GTTTGAGGACATGGGCAAAT+TGG 0.252413 4_2:+12875934 Msa0589690:CDS
TTAGCAACATTGCATTCTTC+TGG 0.260339 4_2:-12874293 None:intergenic
ACACTGATATTCATATAATA+GGG 0.270497 4_2:+12875363 Msa0589690:CDS
AATATTTGATTCCAAGTATC+AGG 0.272544 4_2:-12876655 None:intergenic
AACACTGATATTCATATAAT+AGG 0.276459 4_2:+12875362 Msa0589690:CDS
ATGGGAATCATTTGTTTGTT+TGG 0.277651 4_2:+12874804 Msa0589690:CDS
ATGCTACTATAGAATTCTAT+TGG 0.292225 4_2:+12875318 Msa0589690:CDS
GCAATGTTGCTAATGGAAAA+TGG 0.307320 4_2:+12874303 Msa0589690:CDS
GAGAAATTGCACAGATAAAA+TGG 0.325890 4_2:+12876781 Msa0589690:three_prime_UTR
TGCCTGGTGTTCCTGATACT+TGG 0.327357 4_2:+12876644 Msa0589690:CDS
ATGTGTCACGAATGTCTAAA+TGG 0.345505 4_2:+12876759 Msa0589690:three_prime_UTR
ATGCCTCATGGATTGATGAT+AGG 0.349686 4_2:+12874258 Msa0589690:CDS
GTAATATTGATGACTGTTAC+TGG 0.352240 4_2:-12876502 None:intergenic
ATGATTCTGATTATCTTCAT+TGG 0.353780 4_2:+12874414 Msa0589690:CDS
ATCTCTGAGTATAGAATTGA+TGG 0.356024 4_2:+12876529 Msa0589690:CDS
TGTGGCAAGGTACAATCTTC+TGG 0.373634 4_2:-12874446 None:intergenic
GAAGAATGCAATGTTGCTAA+TGG 0.374390 4_2:+12874296 Msa0589690:CDS
TGGGGAAGTGGTTCAAATAA+AGG 0.377385 4_2:+12876436 Msa0589690:CDS
ACATCAATTGCTTACAACTT+AGG 0.388599 4_2:+12875912 Msa0589690:CDS
TTTGAGGACATGGGCAAATT+GGG 0.394132 4_2:+12875935 Msa0589690:CDS
GAAAATTGTTTATCTATGTA+TGG 0.394541 4_2:-12874371 None:intergenic
GAAACGAGGGCGTGTTCATT+CGG 0.399124 4_2:+12874861 Msa0589690:CDS
TGATTCTGATTATCTTCATT+GGG 0.401619 4_2:+12874415 Msa0589690:CDS
GGAAAGAGGTTGCTATTTGT+AGG 0.411041 4_2:+12874760 Msa0589690:CDS
TCATTTGTTTGTTTGGTTCA+AGG 0.425313 4_2:+12874811 Msa0589690:CDS
CAAATTGTGGTGATAATGAT+TGG 0.439320 4_2:-12873720 None:intergenic
TGTATACATTGGTGTTTGCC+TGG 0.446519 4_2:+12876628 Msa0589690:CDS
GAAAGAGACTTTGTATGTGC+AGG 0.449799 4_2:-12876011 None:intergenic
TGCTACTATAGAATTCTATT+GGG 0.453242 4_2:+12875319 Msa0589690:CDS
GTTCACACATTTGAGAAGGC+TGG 0.454985 4_2:+12876890 Msa0589690:three_prime_UTR
ACAACTTAGGTTTGAGGACA+TGG 0.459680 4_2:+12875925 Msa0589690:CDS
AAGAAGAATCATTGGGGAAG+TGG 0.464968 4_2:+12876424 Msa0589690:CDS
TGCACAGATAAAATGGTCAT+TGG 0.473364 4_2:+12876788 Msa0589690:three_prime_UTR
GTGTTGGACTCCACAAGAAA+TGG 0.485744 4_2:-12875344 None:intergenic
CTGATTATCTTCATTGGGAG+TGG 0.489689 4_2:+12874420 Msa0589690:CDS
AACAAACAAATGATTCCCAT+TGG 0.489762 4_2:-12874801 None:intergenic
TTGCTTACAACTTAGGTTTG+AGG 0.491498 4_2:+12875919 Msa0589690:CDS
TCATTTAGCAATGGAGAAGA+AGG 0.493238 4_2:+12875873 Msa0589690:CDS
TGTATACAATCAGCATTCAA+TGG 0.496978 4_2:-12876613 None:intergenic
TTCACTTCAAAGAAACCAAT+GGG 0.502786 4_2:+12874786 Msa0589690:CDS
TTCACACATTTGAGAAGGCT+GGG 0.505070 4_2:+12876891 Msa0589690:three_prime_UTR
AGAAGCTTCATTGTTTCTGA+AGG 0.506352 4_2:-12873678 None:intergenic
TTTCTTGTGTCAAGAATGGA+AGG 0.513133 4_2:+12874390 Msa0589690:CDS
TGCTGTTCACACATTTGAGA+AGG 0.528843 4_2:+12876886 Msa0589690:three_prime_UTR
TCAGTTTATAGTGAAATTGG+TGG 0.530392 4_2:+12876559 Msa0589690:CDS
TCTGAAGGGTTCATGAGGAA+TGG 0.538014 4_2:-12873663 None:intergenic
TATTATATGAATATCAGTGT+TGG 0.544599 4_2:-12875360 None:intergenic
TGGCAAATTGTTAACTGAAG+AGG 0.546250 4_2:+12876579 Msa0589690:CDS
GCTGATTGGGGAAACAAGAA+TGG 0.550187 4_2:+12876367 Msa0589690:CDS
ATTCACTTCAAAGAAACCAA+TGG 0.554552 4_2:+12874785 Msa0589690:CDS
CTATTGGGCACCATTTCTTG+TGG 0.559058 4_2:+12875334 Msa0589690:CDS
ATGATGAAATATTGTTGAGA+GGG 0.571444 4_2:-12873584 None:intergenic
GAAGCTTCATTGTTTCTGAA+GGG 0.571726 4_2:-12873677 None:intergenic
CAACTTAGGTTTGAGGACAT+GGG 0.573906 4_2:+12875926 Msa0589690:CDS
CACTGATATTCATATAATAG+GGG 0.588475 4_2:+12875364 Msa0589690:CDS
TTGTTTCTGAAGGGTTCATG+AGG 0.592229 4_2:-12873668 None:intergenic
GAGGACATGGGCAAATTGGG+TGG 0.596044 4_2:+12875938 Msa0589690:CDS
GATGATGAAATATTGTTGAG+AGG 0.598076 4_2:-12873585 None:intergenic
TTTGAATTTGAAAATTGAGG+TGG 0.604238 4_2:-12873796 None:intergenic
AATTGTTAACTGAAGAGGAA+AGG 0.606297 4_2:+12876584 Msa0589690:CDS
TTCCAAGTATCAGGAACACC+AGG 0.609005 4_2:-12876646 None:intergenic
GAAGAGAAGAATACAAATTG+TGG 0.612190 4_2:-12873733 None:intergenic
ACCTATGTTTGGTGGATGAG+TGG 0.613388 4_2:+12875470 Msa0589690:CDS
AAATAAAGGTATGATGAGTG+TGG 0.622659 4_2:+12876450 Msa0589690:CDS
TCATTCGGTCTTCAAAGCCA+AGG 0.623097 4_2:+12874876 Msa0589690:CDS
ACCAATTTCACTATAAACTG+AGG 0.625420 4_2:-12876557 None:intergenic
AAACATAGGTATTGAAAACA+AGG 0.626013 4_2:-12875457 None:intergenic
CAATAGTCTTGTATGCTGAG+AGG 0.630162 4_2:+12873757 Msa0589690:CDS
TAATAGGGGATCCAAAGAAG+AGG 0.630639 4_2:+12875378 Msa0589690:CDS
TGAGAAAACTTACTGTGGCA+AGG 0.635156 4_2:-12874459 None:intergenic
ACCACTCATCCACCAAACAT+AGG 0.637859 4_2:-12875471 None:intergenic
AGGTGGGGCTCATTTAGCAA+TGG 0.642952 4_2:+12875864 Msa0589690:intron
CATTTAGCAATGGAGAAGAA+GGG 0.655427 4_2:+12875874 Msa0589690:CDS
TGAATGCTGATTGTATACAT+TGG 0.661517 4_2:+12876617 Msa0589690:CDS
AGAAGAAATCAATGAAACGA+GGG 0.668954 4_2:+12874848 Msa0589690:CDS
AAGAAGAAATCAATGAAACG+AGG 0.699109 4_2:+12874847 Msa0589690:CDS
AAACCTATCATCAATCCATG+AGG 0.704633 4_2:-12874261 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATTTTTATTTTTTGAGT+AGG - chr4_2:12873557-12873576 None:intergenic 10.0%
!!! ATATTTTCTCAAATTTTTAA+TGG + chr4_2:12874593-12874612 Msa0589690:intron 10.0%
!! AATCACAATTAAAAAAAGAA+TGG - chr4_2:12873867-12873886 None:intergenic 15.0%
!!! AATAACCTCAATTATTTTTT+TGG + chr4_2:12873962-12873981 Msa0589690:intron 15.0%
!! TTATTCCAAAAAAATAATTG+AGG - chr4_2:12873970-12873989 None:intergenic 15.0%
!!! TCAATTATTTTTTTGGAATA+AGG + chr4_2:12873969-12873988 Msa0589690:intron 15.0%
!!! AATTACAAACTTTAAAATGT+AGG + chr4_2:12875844-12875863 Msa0589690:intron 15.0%
!! TGTTTATTTGAATTTCATTA+CGG + chr4_2:12876231-12876250 Msa0589690:intron 15.0%
!!! TGTTTTGAATTTGAAAATTG+AGG - chr4_2:12873802-12873821 None:intergenic 20.0%
!! GAAAATTGTTTATCTATGTA+TGG - chr4_2:12874374-12874393 None:intergenic 20.0%
!!! TCACGTTTTCTAATTAAAAT+TGG + chr4_2:12874957-12874976 Msa0589690:intron 20.0%
!!! GTTTTCTAATTAAAATTGGA+CGG + chr4_2:12874961-12874980 Msa0589690:intron 20.0%
!!! TTAAATACAAGAAAAGTACT+AGG + chr4_2:12875227-12875246 Msa0589690:intron 20.0%
!! TATTATATGAATATCAGTGT+TGG - chr4_2:12875363-12875382 None:intergenic 20.0%
!! AACACTGATATTCATATAAT+AGG + chr4_2:12875362-12875381 Msa0589690:CDS 20.0%
!! ACACTGATATTCATATAATA+GGG + chr4_2:12875363-12875382 Msa0589690:CDS 20.0%
!!! ATTTTTATTATCCTCTTCTT+TGG - chr4_2:12875392-12875411 None:intergenic 20.0%
!!! TTTAAAATTATTAGCTGTGT+CGG + chr4_2:12875644-12875663 Msa0589690:intron 20.0%
!!! TACTTGATCTTTTTTCTTTA+TGG + chr4_2:12875759-12875778 Msa0589690:intron 20.0%
!!! TTCATCAACATTTTATCTTT+AGG + chr4_2:12875791-12875810 Msa0589690:intron 20.0%
!!! TTTCATTTTCTTGATAACTT+TGG - chr4_2:12876479-12876498 None:intergenic 20.0%
!!! AACTTTTGTTAAATCTAGAA+AGG + chr4_2:12876826-12876845 Msa0589690:three_prime_UTR 20.0%
!!! ACTTTTGTTAAATCTAGAAA+GGG + chr4_2:12876827-12876846 Msa0589690:three_prime_UTR 20.0%
! ATGATGAAATATTGTTGAGA+GGG - chr4_2:12873587-12873606 None:intergenic 25.0%
! TTTGAATTTGAAAATTGAGG+TGG - chr4_2:12873799-12873818 None:intergenic 25.0%
!!! CCTTTTTAGTTTCAGTTTTA+AGG - chr4_2:12874085-12874104 None:intergenic 25.0%
! CCTTAAAACTGAAACTAAAA+AGG + chr4_2:12874082-12874101 Msa0589690:intron 25.0%
!!! AGAATATGATGAGTTAGTTT+TGG - chr4_2:12874139-12874158 None:intergenic 25.0%
! AACTGGTAAAGAAAAAAAGA+AGG - chr4_2:12874176-12874195 None:intergenic 25.0%
! ATTATTAAGAAGAGTCAAAC+TGG - chr4_2:12874193-12874212 None:intergenic 25.0%
! ATGATTCTGATTATCTTCAT+TGG + chr4_2:12874414-12874433 Msa0589690:CDS 25.0%
! TGATTCTGATTATCTTCATT+GGG + chr4_2:12874415-12874434 Msa0589690:CDS 25.0%
!!! CATATTCTAAGATTGAACAT+GGG - chr4_2:12874987-12875006 None:intergenic 25.0%
!!! TCATATTCTAAGATTGAACA+TGG - chr4_2:12874988-12875007 None:intergenic 25.0%
! ATGCTACTATAGAATTCTAT+TGG + chr4_2:12875318-12875337 Msa0589690:CDS 25.0%
! TGCTACTATAGAATTCTATT+GGG + chr4_2:12875319-12875338 Msa0589690:CDS 25.0%
! CACTGATATTCATATAATAG+GGG + chr4_2:12875364-12875383 Msa0589690:CDS 25.0%
!!! AAACATAGGTATTGAAAACA+AGG - chr4_2:12875460-12875479 None:intergenic 25.0%
!! TTGTTTTCAATACCTATGTT+TGG + chr4_2:12875459-12875478 Msa0589690:CDS 25.0%
!!! TACAAACTTTAAAATGTAGG+TGG + chr4_2:12875847-12875866 Msa0589690:intron 25.0%
!!! ACAAACTTTAAAATGTAGGT+GGG + chr4_2:12875848-12875867 Msa0589690:intron 25.0%
! AGTGAAAAAGAAGAATCATT+GGG + chr4_2:12876417-12876436 Msa0589690:CDS 25.0%
! AATATTTGATTCCAAGTATC+AGG - chr4_2:12876658-12876677 None:intergenic 25.0%
!! ACAGCAATTTTCTAATCTTT+TGG - chr4_2:12876872-12876891 None:intergenic 25.0%
GATGATGAAATATTGTTGAG+AGG - chr4_2:12873588-12873607 None:intergenic 30.0%
! CAAATTGTGGTGATAATGAT+TGG - chr4_2:12873723-12873742 None:intergenic 30.0%
GAAGAGAAGAATACAAATTG+TGG - chr4_2:12873736-12873755 None:intergenic 30.0%
!! TTTGATTTGATGTGCTTTCT+AGG - chr4_2:12873828-12873847 None:intergenic 30.0%
AAACTGAAACTAAAAAGGAG+CGG + chr4_2:12874087-12874106 Msa0589690:intron 30.0%
TGGTAAAGAAAAAAAGAAGG+AGG - chr4_2:12874173-12874192 None:intergenic 30.0%
! TCAATTTTTCTGTCATCTGT+TGG - chr4_2:12874224-12874243 None:intergenic 30.0%
AACAGATGACAGAAAAATTG+AGG + chr4_2:12874223-12874242 Msa0589690:intron 30.0%
! CAGCTTTTATGTGAGTATAA+AGG - chr4_2:12874350-12874369 None:intergenic 30.0%
! CAATTTTCTTGTGTCAAGAA+TGG + chr4_2:12874386-12874405 Msa0589690:CDS 30.0%
CTCATAGACTACTACATATT+TGG + chr4_2:12874700-12874719 Msa0589690:intron 30.0%
ATTCACTTCAAAGAAACCAA+TGG + chr4_2:12874785-12874804 Msa0589690:CDS 30.0%
TTCACTTCAAAGAAACCAAT+GGG + chr4_2:12874786-12874805 Msa0589690:CDS 30.0%
AACAAACAAATGATTCCCAT+TGG - chr4_2:12874804-12874823 None:intergenic 30.0%
ATGGGAATCATTTGTTTGTT+TGG + chr4_2:12874804-12874823 Msa0589690:CDS 30.0%
! TCATTTGTTTGTTTGGTTCA+AGG + chr4_2:12874811-12874830 Msa0589690:CDS 30.0%
!! TTCATTGATTTCTTCTTTGC+AGG - chr4_2:12874844-12874863 None:intergenic 30.0%
AAGAAGAAATCAATGAAACG+AGG + chr4_2:12874847-12874866 Msa0589690:CDS 30.0%
AGAAGAAATCAATGAAACGA+GGG + chr4_2:12874848-12874867 Msa0589690:CDS 30.0%
AAGCTAAATTCCAATTACCT+TGG - chr4_2:12874896-12874915 None:intergenic 30.0%
! TGTGACTATTTTCTACTACA+CGG - chr4_2:12874919-12874938 None:intergenic 30.0%
TGAATCTACATGTTTGACAT+TGG - chr4_2:12875152-12875171 None:intergenic 30.0%
! GCTTTTATTGTTCAGTTTCA+AGG - chr4_2:12875257-12875276 None:intergenic 30.0%
! TGTGTATTTTCATGTCATCT+AGG + chr4_2:12875289-12875308 Msa0589690:intron 30.0%
! TTTTCAATACCTATGTTTGG+TGG + chr4_2:12875462-12875481 Msa0589690:CDS 30.0%
! TTCAGTGTAATTAGAAGTGT+CGG - chr4_2:12875612-12875631 None:intergenic 30.0%
!! CAAACTTTAAAATGTAGGTG+GGG + chr4_2:12875849-12875868 Msa0589690:intron 30.0%
ACATCAATTGCTTACAACTT+AGG + chr4_2:12875912-12875931 Msa0589690:CDS 30.0%
!!! AAGAAGACTCTAGTTTTGTT+TGG - chr4_2:12875978-12875997 None:intergenic 30.0%
TTGTCAAATAGCAGTTATAG+CGG - chr4_2:12876279-12876298 None:intergenic 30.0%
!! CAATGATTCTTCTTTTTCAC+TGG - chr4_2:12876418-12876437 None:intergenic 30.0%
CAGTGAAAAAGAAGAATCAT+TGG + chr4_2:12876416-12876435 Msa0589690:CDS 30.0%
GTGAAAAAGAAGAATCATTG+GGG + chr4_2:12876418-12876437 Msa0589690:CDS 30.0%
! AAATAAAGGTATGATGAGTG+TGG + chr4_2:12876450-12876469 Msa0589690:CDS 30.0%
! GTAATATTGATGACTGTTAC+TGG - chr4_2:12876505-12876524 None:intergenic 30.0%
ATCTCTGAGTATAGAATTGA+TGG + chr4_2:12876529-12876548 Msa0589690:CDS 30.0%
TCCTCAGTTTATAGTGAAAT+TGG + chr4_2:12876556-12876575 Msa0589690:CDS 30.0%
ACCAATTTCACTATAAACTG+AGG - chr4_2:12876560-12876579 None:intergenic 30.0%
TCAGTTTATAGTGAAATTGG+TGG + chr4_2:12876559-12876578 Msa0589690:CDS 30.0%
AATTGTTAACTGAAGAGGAA+AGG + chr4_2:12876584-12876603 Msa0589690:CDS 30.0%
TGTATACAATCAGCATTCAA+TGG - chr4_2:12876616-12876635 None:intergenic 30.0%
! TGAATGCTGATTGTATACAT+TGG + chr4_2:12876617-12876636 Msa0589690:CDS 30.0%
CATCTTCATGTCACAAAAAA+GGG - chr4_2:12876714-12876733 None:intergenic 30.0%
ACATCTTCATGTCACAAAAA+AGG - chr4_2:12876715-12876734 None:intergenic 30.0%
GAGAAATTGCACAGATAAAA+TGG + chr4_2:12876781-12876800 Msa0589690:three_prime_UTR 30.0%
!! GAAGCTTCATTGTTTCTGAA+GGG - chr4_2:12873680-12873699 None:intergenic 35.0%
!! AGAAGCTTCATTGTTTCTGA+AGG - chr4_2:12873681-12873700 None:intergenic 35.0%
TCAGAAACAATGAAGCTTCT+TGG + chr4_2:12873681-12873700 Msa0589690:CDS 35.0%
TGACAGAAAAATTGAGGATG+TGG + chr4_2:12874229-12874248 Msa0589690:CDS 35.0%
!!! ATGTGGTTTTAAATGCCTCA+TGG + chr4_2:12874246-12874265 Msa0589690:CDS 35.0%
AAACCTATCATCAATCCATG+AGG - chr4_2:12874264-12874283 None:intergenic 35.0%
TTAGCAACATTGCATTCTTC+TGG - chr4_2:12874296-12874315 None:intergenic 35.0%
GAAGAATGCAATGTTGCTAA+TGG + chr4_2:12874296-12874315 Msa0589690:CDS 35.0%
GCAATGTTGCTAATGGAAAA+TGG + chr4_2:12874303-12874322 Msa0589690:CDS 35.0%
TTTCTTGTGTCAAGAATGGA+AGG + chr4_2:12874390-12874409 Msa0589690:CDS 35.0%
TGTAGTAGTCTATGAGAATG+CGG - chr4_2:12874696-12874715 None:intergenic 35.0%
! CTTTCCTTCAAGCTTTTTGA+GGG - chr4_2:12874746-12874765 None:intergenic 35.0%
! TCTTTCCTTCAAGCTTTTTG+AGG - chr4_2:12874747-12874766 None:intergenic 35.0%
TCAAAAAGCTTGAAGGAAAG+AGG + chr4_2:12874746-12874765 Msa0589690:CDS 35.0%
GTCATTTGAACTGAATCGAA+AGG - chr4_2:12875075-12875094 None:intergenic 35.0%
TTCAAATGACTCGAATTGCA+TGG + chr4_2:12875085-12875104 Msa0589690:intron 35.0%
ATCTAACCTTTCTATACAGC+AGG + chr4_2:12875116-12875135 Msa0589690:intron 35.0%
TCATTTAGCAATGGAGAAGA+AGG + chr4_2:12875873-12875892 Msa0589690:CDS 35.0%
CATTTAGCAATGGAGAAGAA+GGG + chr4_2:12875874-12875893 Msa0589690:CDS 35.0%
TTGCTTACAACTTAGGTTTG+AGG + chr4_2:12875919-12875938 Msa0589690:CDS 35.0%
!!! TGTTTTTCAGAAGTGCTGAT+TGG + chr4_2:12876353-12876372 Msa0589690:intron 35.0%
!!! GTTTTTCAGAAGTGCTGATT+GGG + chr4_2:12876354-12876373 Msa0589690:intron 35.0%
!!! TTTTTCAGAAGTGCTGATTG+GGG + chr4_2:12876355-12876374 Msa0589690:intron 35.0%
TGGCAAATTGTTAACTGAAG+AGG + chr4_2:12876579-12876598 Msa0589690:CDS 35.0%
ATGTGTCACGAATGTCTAAA+TGG + chr4_2:12876759-12876778 Msa0589690:three_prime_UTR 35.0%
TGCACAGATAAAATGGTCAT+TGG + chr4_2:12876788-12876807 Msa0589690:three_prime_UTR 35.0%
!! TTGTTTCTGAAGGGTTCATG+AGG - chr4_2:12873671-12873690 None:intergenic 40.0%
!! CAATAGTCTTGTATGCTGAG+AGG + chr4_2:12873757-12873776 Msa0589690:CDS 40.0%
! ATGCCTCATGGATTGATGAT+AGG + chr4_2:12874258-12874277 Msa0589690:CDS 40.0%
CTGATTATCTTCATTGGGAG+TGG + chr4_2:12874420-12874439 Msa0589690:CDS 40.0%
TGAGAAAACTTACTGTGGCA+AGG - chr4_2:12874462-12874481 None:intergenic 40.0%
GAGTGTGAGAAAACTTACTG+TGG - chr4_2:12874467-12874486 None:intergenic 40.0%
TCTATGTCGCACCAATACTT+TGG + chr4_2:12874512-12874531 Msa0589690:intron 40.0%
TGGTCCAAACTCCAAAGTAT+TGG - chr4_2:12874526-12874545 None:intergenic 40.0%
!! TACTTTGGAGTTTGGACCAA+AGG + chr4_2:12874527-12874546 Msa0589690:intron 40.0%
TGTAACCACATGTTACGTGT+CGG - chr4_2:12874562-12874581 None:intergenic 40.0%
GGCTTAAGTTAGGTCAAAAG+TGG - chr4_2:12874675-12874694 None:intergenic 40.0%
ATGAGAATGCGGCTTAAGTT+AGG - chr4_2:12874685-12874704 None:intergenic 40.0%
ATAGCCCTCAAAAAGCTTGA+AGG + chr4_2:12874739-12874758 Msa0589690:CDS 40.0%
!! GGAAAGAGGTTGCTATTTGT+AGG + chr4_2:12874760-12874779 Msa0589690:CDS 40.0%
GTCTTCAAAGCCAAGGTAAT+TGG + chr4_2:12874883-12874902 Msa0589690:intron 40.0%
AGATTGCCTGCTGTATAGAA+AGG - chr4_2:12875125-12875144 None:intergenic 40.0%
TTTCTATACAGCAGGCAATC+TGG + chr4_2:12875124-12875143 Msa0589690:intron 40.0%
TAATAGGGGATCCAAAGAAG+AGG + chr4_2:12875378-12875397 Msa0589690:CDS 40.0%
TCACTGAGCGTGCAAAAAAT+TGG + chr4_2:12875420-12875439 Msa0589690:CDS 40.0%
TTCATAGGGTAGGTATGAAG+AGG - chr4_2:12875520-12875539 None:intergenic 40.0%
GGTACTATGAAGCATGGATA+CGG - chr4_2:12875705-12875724 None:intergenic 40.0%
TAGGTAGGTACTATGAAGCA+TGG - chr4_2:12875711-12875730 None:intergenic 40.0%
AAGTAAGTAGATAGCCAGGT+AGG - chr4_2:12875730-12875749 None:intergenic 40.0%
CTACAAGTAAGTAGATAGCC+AGG - chr4_2:12875734-12875753 None:intergenic 40.0%
CTGGCTATCTACTTACTTGT+AGG + chr4_2:12875732-12875751 Msa0589690:intron 40.0%
ACAACTTAGGTTTGAGGACA+TGG + chr4_2:12875925-12875944 Msa0589690:CDS 40.0%
CAACTTAGGTTTGAGGACAT+GGG + chr4_2:12875926-12875945 Msa0589690:CDS 40.0%
TTTGAGGACATGGGCAAATT+GGG + chr4_2:12875935-12875954 Msa0589690:CDS 40.0%
GAAAGAGACTTTGTATGTGC+AGG - chr4_2:12876014-12876033 None:intergenic 40.0%
AAGAAGAATCATTGGGGAAG+TGG + chr4_2:12876424-12876443 Msa0589690:CDS 40.0%
TGGGGAAGTGGTTCAAATAA+AGG + chr4_2:12876436-12876455 Msa0589690:CDS 40.0%
! TGTATACATTGGTGTTTGCC+TGG + chr4_2:12876628-12876647 Msa0589690:CDS 40.0%
TGCTGTTCACACATTTGAGA+AGG + chr4_2:12876886-12876905 Msa0589690:three_prime_UTR 40.0%
TTCACACATTTGAGAAGGCT+GGG + chr4_2:12876891-12876910 Msa0589690:three_prime_UTR 40.0%
TCTGAAGGGTTCATGAGGAA+TGG - chr4_2:12873666-12873685 None:intergenic 45.0%
TGTGGCAAGGTACAATCTTC+TGG - chr4_2:12874449-12874468 None:intergenic 45.0%
!! CGCACCAATACTTTGGAGTT+TGG + chr4_2:12874519-12874538 Msa0589690:intron 45.0%
GTGTCGGATAGATACACCTT+TGG - chr4_2:12874546-12874565 None:intergenic 45.0%
TCTATCCGACACGTAACATG+TGG + chr4_2:12874554-12874573 Msa0589690:intron 45.0%
!! AGCTTTTTGAGGGCTATCTC+TGG - chr4_2:12874736-12874755 None:intergenic 45.0%
TCATTCGGTCTTCAAAGCCA+AGG + chr4_2:12874876-12874895 Msa0589690:CDS 45.0%
CTATTGGGCACCATTTCTTG+TGG + chr4_2:12875334-12875353 Msa0589690:CDS 45.0%
GTGTTGGACTCCACAAGAAA+TGG - chr4_2:12875347-12875366 None:intergenic 45.0%
GAGCGTGCAAAAAATTGGAC+TGG + chr4_2:12875425-12875444 Msa0589690:CDS 45.0%
! ACCTATGTTTGGTGGATGAG+TGG + chr4_2:12875470-12875489 Msa0589690:CDS 45.0%
ACCACTCATCCACCAAACAT+AGG - chr4_2:12875474-12875493 None:intergenic 45.0%
CATACCTACCCTATGAAGCA+TGG + chr4_2:12875523-12875542 Msa0589690:intron 45.0%
!! AGGAGTGTCCATGCTTCATA+GGG - chr4_2:12875534-12875553 None:intergenic 45.0%
TATGAAGCATGGACACTCCT+CGG + chr4_2:12875534-12875553 Msa0589690:intron 45.0%
ATGAAGCATGGATACGGACA+CGG - chr4_2:12875699-12875718 None:intergenic 45.0%
CTTCATAGTACCTACCTACC+TGG + chr4_2:12875713-12875732 Msa0589690:intron 45.0%
AAGTAGATAGCCAGGTAGGT+AGG - chr4_2:12875726-12875745 None:intergenic 45.0%
GTTTGAGGACATGGGCAAAT+TGG + chr4_2:12875934-12875953 Msa0589690:CDS 45.0%
CGAAGCAATGTTGTCAATCG+TGG + chr4_2:12876090-12876109 Msa0589690:intron 45.0%
GCTGATTGGGGAAACAAGAA+TGG + chr4_2:12876367-12876386 Msa0589690:CDS 45.0%
TTCCAAGTATCAGGAACACC+AGG - chr4_2:12876649-12876668 None:intergenic 45.0%
GTTCACACATTTGAGAAGGC+TGG + chr4_2:12876890-12876909 Msa0589690:three_prime_UTR 45.0%
! CGACATGTAAGTGTCGTGTC+CGG + chr4_2:12874619-12874638 Msa0589690:intron 50.0%
! GAAACGAGGGCGTGTTCATT+CGG + chr4_2:12874861-12874880 Msa0589690:CDS 50.0%
GTGTCCATGCTTCATAGGGT+AGG - chr4_2:12875530-12875549 None:intergenic 50.0%
!! GAGGAGTGTCCATGCTTCAT+AGG - chr4_2:12875535-12875554 None:intergenic 50.0%
!! TAGAAGTGTCGGTGTCGTGT+CGG - chr4_2:12875601-12875620 None:intergenic 50.0%
! TATTAGCTGTGTCGGCGTGT+CGG + chr4_2:12875652-12875671 Msa0589690:intron 50.0%
AGGTGGGGCTCATTTAGCAA+TGG + chr4_2:12875864-12875883 Msa0589690:intron 50.0%
TGCCTGGTGTTCCTGATACT+TGG + chr4_2:12876644-12876663 Msa0589690:CDS 50.0%
! CATGTAAGTGTCGTGTCCGG+TGG + chr4_2:12874622-12874641 Msa0589690:intron 55.0%
TGCATCACCGACACAACCAC+CGG - chr4_2:12874641-12874660 None:intergenic 55.0%
GCATGGACACTCCTCGGATT+AGG + chr4_2:12875540-12875559 Msa0589690:intron 55.0%
ACACGACACGTGTTCGACAC+CGG - chr4_2:12875572-12875591 None:intergenic 55.0%
ATGGATACGGACACGGACAC+CGG - chr4_2:12875692-12875711 None:intergenic 55.0%
GAGGACATGGGCAAATTGGG+TGG + chr4_2:12875938-12875957 Msa0589690:CDS 55.0%
GTCGTGTCCGGTGGTTGTGT+CGG + chr4_2:12874631-12874650 Msa0589690:intron 60.0%
! TCCTCGGATTAGGCGTGTCC+CGG + chr4_2:12875550-12875569 Msa0589690:intron 60.0%
CACGACACGTGTTCGACACC+GGG - chr4_2:12875571-12875590 None:intergenic 60.0%
!! TGTCGGTGTCGTGTCGGTGT+CGG - chr4_2:12875595-12875614 None:intergenic 60.0%
ACCGGGACACGCCTAATCCG+AGG - chr4_2:12875554-12875573 None:intergenic 65.0%
! GTCGGTGTCCGTGTCGTGTC+CGG + chr4_2:12875670-12875689 Msa0589690:intron 65.0%
CACGGACACCGGACACGACA+CGG - chr4_2:12875681-12875700 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 12873541 12876938 12873541 ID=Msa0589690;Name=Msa0589690
chr4_2 mRNA 12873541 12876938 12873541 ID=Msa0589690-mRNA-1;Parent=Msa0589690;Name=Msa0589690-mRNA-1;_AED=0.08;_eAED=0.08;_QI=131|1|1|1|1|1|6|245|427
chr4_2 exon 12873541 12873852 12873541 ID=Msa0589690-mRNA-1:exon:3715;Parent=Msa0589690-mRNA-1
chr4_2 exon 12874228 12874468 12874228 ID=Msa0589690-mRNA-1:exon:3716;Parent=Msa0589690-mRNA-1
chr4_2 exon 12874726 12874897 12874726 ID=Msa0589690-mRNA-1:exon:3717;Parent=Msa0589690-mRNA-1
chr4_2 exon 12875311 12875510 12875311 ID=Msa0589690-mRNA-1:exon:3718;Parent=Msa0589690-mRNA-1
chr4_2 exon 12875866 12876024 12875866 ID=Msa0589690-mRNA-1:exon:3719;Parent=Msa0589690-mRNA-1
chr4_2 exon 12876363 12876938 12876363 ID=Msa0589690-mRNA-1:exon:3720;Parent=Msa0589690-mRNA-1
chr4_2 five_prime_UTR 12873541 12873671 12873541 ID=Msa0589690-mRNA-1:five_prime_utr;Parent=Msa0589690-mRNA-1
chr4_2 CDS 12873672 12873852 12873672 ID=Msa0589690-mRNA-1:cds;Parent=Msa0589690-mRNA-1
chr4_2 CDS 12874228 12874468 12874228 ID=Msa0589690-mRNA-1:cds;Parent=Msa0589690-mRNA-1
chr4_2 CDS 12874726 12874897 12874726 ID=Msa0589690-mRNA-1:cds;Parent=Msa0589690-mRNA-1
chr4_2 CDS 12875311 12875510 12875311 ID=Msa0589690-mRNA-1:cds;Parent=Msa0589690-mRNA-1
chr4_2 CDS 12875866 12876024 12875866 ID=Msa0589690-mRNA-1:cds;Parent=Msa0589690-mRNA-1
chr4_2 CDS 12876363 12876693 12876363 ID=Msa0589690-mRNA-1:cds;Parent=Msa0589690-mRNA-1
chr4_2 three_prime_UTR 12876694 12876938 12876694 ID=Msa0589690-mRNA-1:three_prime_utr;Parent=Msa0589690-mRNA-1
Gene Sequence

>Msa0589690

ATGAACCCTTCAGAAACAATGAAGCTTCTTGGAAAAAAGCTTTCTTTTCCAATCATTATCACCACAATTTGTATTCTTCTCTTCACAATAGTCTTGTATGCTGAGAGGCTTAGTTTCATTTCTTCCACCTCAATTTTCAAATTCAAAACATGTCCTAGAAAGCACATCAAATCAAAATCTAATGACAGAAAAATTGAGGATGTGGTTTTAAATGCCTCATGGATTGATGATAGGTTTGATTTTGATCCAGAAGAATGCAATGTTGCTAATGGAAAATGGATATTTAACAATACAATCAAGCCTTTATACTCACATAAAAGCTGTCCATACATAGATAAACAATTTTCTTGTGTCAAGAATGGAAGGAATGATTCTGATTATCTTCATTGGGAGTGGCAGCCAGAAGATTGTACCTTGCCACAATTTAATCCAGAGATAGCCCTCAAAAAGCTTGAAGGAAAGAGGTTGCTATTTGTAGGAGATTCACTTCAAAGAAACCAATGGGAATCATTTGTTTGTTTGGTTCAAGGTATCATACCTGCAAAGAAGAAATCAATGAAACGAGGGCGTGTTCATTCGGTCTTCAAAGCCAAGGAATATAATGCTACTATAGAATTCTATTGGGCACCATTTCTTGTGGAGTCCAACACTGATATTCATATAATAGGGGATCCAAAGAAGAGGATAATAAAAATTGATGAAATCACTGAGCGTGCAAAAAATTGGACTGGAGCTGATATCCTTGTTTTCAATACCTATGTTTGGTGGATGAGTGGTCTTAGAGTTAAAGCATTGTGGGGCTCATTTAGCAATGGAGAAGAAGGGTATGAAGAACTTGATACATCAATTGCTTACAACTTAGGTTTGAGGACATGGGCAAATTGGGTGGACTCAACTATCAATCCAAACAAAACTAGAGTCTTCTTCACAACTATGTCTCCTGCACATACAAAAAGTGCTGATTGGGGAAACAAGAATGGTACAAAATGTTTCAATGAAACAATGCCAGTGAAAAAGAAGAATCATTGGGGAAGTGGTTCAAATAAAGGTATGATGAGTGTGGTAGCCAAAGTTATCAAGAAAATGAAAGTTCCAGTAACAGTCATCAATATTACACAAATCTCTGAGTATAGAATTGATGGTCATTCCTCAGTTTATAGTGAAATTGGTGGCAAATTGTTAACTGAAGAGGAAAGGACTAATCCATTGAATGCTGATTGTATACATTGGTGTTTGCCTGGTGTTCCTGATACTTGGAATCAAATATTTTTTGCAATGTTGTGA
Protein sequence

>Msa0589690

MNPSETMKLLGKKLSFPIIITTICILLFTIVLYAERLSFISSTSIFKFKTCPRKHIKSKSNDRKIEDVVLNASWIDDRFDFDPEECNVANGKWIFNNTIKPLYSHKSCPYIDKQFSCVKNGRNDSDYLHWEWQPEDCTLPQFNPEIALKKLEGKRLLFVGDSLQRNQWESFVCLVQGIIPAKKKSMKRGRVHSVFKAKEYNATIEFYWAPFLVESNTDIHIIGDPKKRIIKIDEITERAKNWTGADILVFNTYVWWMSGLRVKALWGSFSNGEEGYEELDTSIAYNLGLRTWANWVDSTINPNKTRVFFTTMSPAHTKSADWGNKNGTKCFNETMPVKKKNHWGSGSNKGMMSVVAKVIKKMKVPVTVINITQISEYRIDGHSSVYSEIGGKLLTEEERTNPLNADCIHWCLPGVPDTWNQIFFAML*