AlfalfaGEDB | Msa1327650

M. sativa cultivar zhongmu-4 / Msa1327650

KEGG: K01246
GO: -
PFAM: PF03352.16

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1327650	sp\|P44321\|3MGA_HAEIN	42.222	180	100	3	116	294	4	180	1.33e-45	155

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1327650	A0A396GM12	96.825	315	6	2	1	311	1	315	0.0	627

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0174450	Msa1327650	0.814574	1.437679e-51	-8.615850e-47
Msa0193850	Msa1327650	0.809261	2.064706e-50	-8.615850e-47
Msa0199960	Msa1327650	0.839515	1.513242e-57	-8.615850e-47
Msa0235710	Msa1327650	0.811868	5.646101e-51	-8.615850e-47
Msa0243600	Msa1327650	0.828192	1.030423e-54	-8.615850e-47
Msa0274440	Msa1327650	0.815483	9.032602e-52	-8.615850e-47
Msa0282220	Msa1327650	0.839953	1.163786e-57	-8.615850e-47
Msa0314170	Msa1327650	0.853715	1.989715e-61	-8.615850e-47
Msa0389500	Msa1327650	0.825801	3.844102e-54	-8.615850e-47
Msa0392380	Msa1327650	0.839586	1.450369e-57	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1327650	MtrunA17_Chr8g0366071	96.825	315	6	2	1	311	1	315	0.0	627
Msa1327650	MtrunA17_Chr5g0420791	67.308	312	97	4	1	311	1	308	5.15e-144	408
Msa1327650	MtrunA17_Chr4g0073991	52.880	191	90	0	115	305	148	338	1.91e-76	238
Msa1327650	MtrunA17_Chr4g0001371	51.337	187	91	0	115	301	137	323	1.83e-72	228
Msa1327650	MtrunA17_Chr2g0309681	48.309	207	96	2	98	304	193	388	3.62e-70	223
Msa1327650	MtrunA17_Chr8g0356301	48.438	192	97	1	113	304	193	382	1.10e-68	218
Msa1327650	MtrunA17_Chr3g0141411	47.208	197	101	2	105	300	120	314	5.18e-65	207

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1327650	AT1G13635.2	62.745	306	111	3	7	311	8	311	1.05e-130	374
Msa1327650	AT1G13635.1	62.745	306	111	3	7	311	8	311	1.05e-130	374
Msa1327650	AT1G75090.1	51.020	196	96	0	111	306	115	310	1.07e-74	233
Msa1327650	AT5G57970.1	51.042	192	94	0	115	306	154	345	3.47e-74	232
Msa1327650	AT5G57970.2	51.042	192	94	0	115	306	154	345	3.47e-74	232
Msa1327650	AT5G44680.1	50.526	190	92	1	115	304	164	351	6.12e-71	224
Msa1327650	AT1G15970.1	48.168	191	99	0	115	305	145	335	4.05e-68	216
Msa1327650	AT1G15970.2	48.168	191	99	0	115	305	145	335	4.05e-68	216
Msa1327650	AT1G80850.1	45.550	191	104	0	115	305	134	324	2.34e-65	208
Msa1327650	AT3G12710.1	42.795	229	127	3	81	307	86	312	9.15e-64	204

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Find 61 sgRNAs with CRISPR-Local

Find 260 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGTGGATTTCGGTTTG+TGG	0.483743	8_4:-44969641	Msa1327650:CDS
AAAAGCAGAGGCTCTAAGCA+AGG	0.611659	8_4:-44969671	Msa1327650:CDS
AAACGTGGATTTCGGTTTGT+GGG	0.497995	8_4:-44969640	Msa1327650:CDS
AAAGTACAAGCGATGAAAAT+TGG	0.290448	8_4:+44969404	None:intergenic
AAATGTAAGAAAACAAGCTT+TGG	0.439042	8_4:-44974064	Msa1327650:CDS
AATGAGATAGATGCTGAAGT+TGG	0.584360	8_4:+44974142	None:intergenic
ACAATCCACAAGATGATCAA+TGG	0.535979	8_4:+44969576	None:intergenic
ACATGGTAAAACGTGGATTT+CGG	0.376776	8_4:-44969648	Msa1327650:CDS
ACCCAAGAAGTGTCCCATTA+AGG	0.321221	8_4:-44969699	Msa1327650:CDS
ACTAGTGACCACTGAGCCTG+GGG	0.626508	8_4:-44973761	Msa1327650:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GGAATTTATGAAAACTTGGA+GGG	+	chr8_4:44969246-44969265	None:intergenic	30.0%
TGGAATTTATGAAAACTTGG+AGG	+	chr8_4:44969247-44969266	None:intergenic	30.0%
GGTTGGAATTTATGAAAACT+TGG	+	chr8_4:44969250-44969269	None:intergenic	30.0%
AACTGGATCACAAAAAATAG+TGG	-	chr8_4:44969686-44969705	Msa1327650:CDS	30.0%
GTCAAATATGTCTCATATTG+CGG	+	chr8_4:44969910-44969929	None:intergenic	30.0%
CTTGATATTGTGAAAAATCG+CGG	+	chr8_4:44969932-44969951	None:intergenic	30.0%
ATATCAAGGATTGCAATGTT+AGG	-	chr8_4:44969944-44969963	Msa1327650:intron	30.0%
TATCAAGGATTGCAATGTTA+GGG	-	chr8_4:44969945-44969964	Msa1327650:intron	30.0%
AGCTCAAAACAACCAATTAA+TGG	+	chr8_4:44970338-44970357	None:intergenic	30.0%
GTGCACAAAAAAAAATCAGA+AGG	-	chr8_4:44970440-44970459	Msa1327650:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	44969224	44974208	44969224	ID=Msa1327650;Name=Msa1327650
chr8_4	mRNA	44969224	44974208	44969224	ID=Msa1327650-mRNA-1;Parent=Msa1327650;Name=Msa1327650-mRNA-1;_AED=0.20;_eAED=0.20;_QI=111\|1\|1\|1\|1\|1\|5\|298\|311
chr8_4	exon	44969224	44969800	44969224	ID=Msa1327650-mRNA-1:exon:12553;Parent=Msa1327650-mRNA-1
chr8_4	exon	44970318	44970459	44970318	ID=Msa1327650-mRNA-1:exon:12552;Parent=Msa1327650-mRNA-1
chr8_4	exon	44970556	44970639	44970556	ID=Msa1327650-mRNA-1:exon:12551;Parent=Msa1327650-mRNA-1
chr8_4	exon	44971046	44971103	44971046	ID=Msa1327650-mRNA-1:exon:12550;Parent=Msa1327650-mRNA-1
chr8_4	exon	44973725	44974208	44973725	ID=Msa1327650-mRNA-1:exon:12549;Parent=Msa1327650-mRNA-1
chr8_4	five_prime_UTR	44974098	44974208	44974098	ID=Msa1327650-mRNA-1:five_prime_utr;Parent=Msa1327650-mRNA-1
chr8_4	CDS	44973725	44974097	44973725	ID=Msa1327650-mRNA-1:cds;Parent=Msa1327650-mRNA-1
chr8_4	CDS	44971046	44971103	44971046	ID=Msa1327650-mRNA-1:cds;Parent=Msa1327650-mRNA-1
chr8_4	CDS	44970556	44970639	44970556	ID=Msa1327650-mRNA-1:cds;Parent=Msa1327650-mRNA-1
chr8_4	CDS	44970318	44970459	44970318	ID=Msa1327650-mRNA-1:cds;Parent=Msa1327650-mRNA-1
chr8_4	CDS	44969522	44969800	44969522	ID=Msa1327650-mRNA-1:cds;Parent=Msa1327650-mRNA-1
chr8_4	three_prime_UTR	44969224	44969521	44969224	ID=Msa1327650-mRNA-1:three_prime_utr;Parent=Msa1327650-mRNA-1

Gene Sequence

>Msa1327650

ATGTCTAAAACAAATGTAAGAAAACAAGCTTTGGAGAGGAGCACAAGTTTCAAAGATACACAAAAAATATTGAACCAAAATTTCTTCCATAATAAAATCTTCAAGAAAGTGTACCCTATTGGACTTCAAAAAAGCACTTCTTCACTATCTTTATCTTCAGTTTCATTATCTTTGTCACAAAACTCAAATGACTCTTCTCAAGCTGATTCTTTGACTCCATTGGATGAAAGGATTTCATCAGCACTTCGTTTGATTTCATCTTCACCACATGAAAGAAGAGAATTTGCAGTTGCAAAAACTACACAACAAAGTCCACTAGTGACCACTGAGCCTGGGGAATTGAAAAGGTGCAACTGGATCACAAAAAATAGTGATAAGCTATACGTAGAATTTCATGATGAATGCTGGGGAGTTCCAGCTTATGACGACAACAAATTGTTTGAGATGCTTGCAATGTCTGGATTGCTCATGGACTACAATTGGACAGAAATTATAAAAAGAAGGGAACCACTTAGAGAAGTTTTTGCTGGATTTGATCCTTACACTGTTGCCAAAATGGAGGAACAGGAAATCATAGAGATAACATCAAACAAAGCACTTTCTTTAGCTGATAGCAGAGTTATGTGCATTGTTGACAATGCCAAATGCATAATGAAGGTTGTTAGGGAATGTGGATCATTTAGTAGCTACATATGGAGTTTTGTAAATCATAAACCAATAATTAACAAATACAAATACCCAAGAAGTGTCCCATTAAGGAGTCCAAAAGCAGAGGCTCTAAGCAAGGACATGGTAAAACGTGGATTTCGGTTTGTGGGACCAGTGATAGTGCACTCTTTTATGCAAGCTGCAGGATTGACCATTGATCATCTTGTGGATTGTTATAGACATAGTGAATGTGTAAGCCTAGCAGAAAGACCTTGGAGGCATATCTAA

Protein sequence

>Msa1327650

MSKTNVRKQALERSTSFKDTQKILNQNFFHNKIFKKVYPIGLQKSTSSLSLSSVSLSLSQNSNDSSQADSLTPLDERISSALRLISSSPHERREFAVAKTTQQSPLVTTEPGELKRCNWITKNSDKLYVEFHDECWGVPAYDDNKLFEMLAMSGLLMDYNWTEIIKRREPLREVFAGFDPYTVAKMEEQEIIEITSNKALSLADSRVMCIVDNAKCIMKVVRECGSFSSYIWSFVNHKPIINKYKYPRSVPLRSPKAEALSKDMVKRGFRFVGPVIVHSFMQAAGLTIDHLVDCYRHSECVSLAERPWRHI*