AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0221000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0221000 MtrunA17_Chr2g0325101 100.000 196 0 0 1 196 1 196 1.31e-147 407
Msa0221000 MtrunA17_Chr4g0037021 84.772 197 29 1 1 196 1 197 1.83e-126 354
Msa0221000 MtrunA17_Chr3g0117931 79.082 196 40 1 2 196 4 199 1.15e-117 332
Msa0221000 MtrunA17_Chr8g0371501 84.831 178 27 0 1 178 1 178 1.26e-116 329
Msa0221000 MtrunA17_Chr6g0486041 77.387 199 41 1 1 195 1 199 1.40e-116 330
Msa0221000 MtrunA17_Chr5g0405791 84.270 178 28 0 1 178 1 178 3.04e-115 326
Msa0221000 MtrunA17_Chr4g0046211 91.608 143 12 0 1 143 1 143 1.11e-97 279
Msa0221000 MtrunA17_Chr7g0265001 32.768 177 105 4 8 181 18 183 2.25e-28 105
Msa0221000 MtrunA17_Chr1g0212521 33.533 167 98 5 6 170 15 170 8.10e-27 101
Msa0221000 MtrunA17_Chr1g0182321 33.533 167 98 5 6 170 15 170 1.41e-26 100
Msa0221000 MtrunA17_Chr4g0030911 33.533 167 98 5 6 170 15 170 1.85e-26 100
Msa0221000 MtrunA17_Chr1g0182301 33.533 167 98 5 6 170 15 170 2.70e-26 100
Msa0221000 MtrunA17_Chr8g0384311 31.844 179 107 5 6 181 13 179 3.47e-26 99.8
Msa0221000 MtrunA17_Chr3g0130271 30.890 191 114 6 7 190 13 192 5.43e-26 99.4
Msa0221000 MtrunA17_Chr7g0263071 32.934 167 99 5 6 170 15 170 6.32e-26 99.4
Msa0221000 MtrunA17_Chr2g0290891 31.977 172 103 5 1 170 1 160 3.95e-25 97.1
Msa0221000 MtrunA17_Chr6g0458081 29.744 195 123 5 1 193 1 183 6.71e-25 96.7
Msa0221000 MtrunA17_Chr1g0159481 31.285 179 108 5 6 181 17 183 1.06e-24 96.3
Msa0221000 MtrunA17_Chr1g0199331 33.708 178 101 7 7 180 32 196 1.26e-24 95.5
Msa0221000 MtrunA17_Chr1g0180291 31.073 177 107 5 8 181 16 180 6.71e-24 94.0
Msa0221000 MtrunA17_Chr4g0055081 35.758 165 92 5 8 170 11 163 1.25e-23 93.2
Msa0221000 MtrunA17_Chr8g0335471 32.000 175 102 6 6 175 8 170 2.06e-22 89.7
Msa0221000 MtrunA17_Chr5g0400131 35.758 165 92 5 8 170 11 163 2.96e-22 89.7
Msa0221000 MtrunA17_Chr8g0381841 35.758 165 92 5 8 170 19 171 4.87e-22 89.4
Msa0221000 MtrunA17_Chr2g0319951 30.939 181 111 5 6 184 13 181 5.44e-22 89.0
Msa0221000 MtrunA17_Chr8g0349711 29.070 172 108 5 1 170 1 160 5.69e-22 89.0
Msa0221000 MtrunA17_Chr2g0327911 30.811 185 113 6 5 185 14 187 5.87e-22 89.0
Msa0221000 MtrunA17_Chr7g0250531 30.769 169 103 5 6 172 8 164 8.95e-22 88.2
Msa0221000 MtrunA17_Chr3g0123971 34.545 165 94 5 8 170 11 163 1.30e-21 87.8
Msa0221000 MtrunA17_Chr8g0382221 34.545 165 94 5 8 170 11 163 1.80e-21 87.4
Msa0221000 MtrunA17_Chr4g0055651 29.586 169 105 5 6 172 8 164 3.01e-21 86.7
Msa0221000 MtrunA17_Chr3g0123821 33.333 165 96 5 8 170 18 170 3.31e-21 87.0
Msa0221000 MtrunA17_Chr7g0231131 34.337 166 95 5 7 170 16 169 3.34e-21 87.0
Msa0221000 MtrunA17_Chr1g0174281 34.940 166 94 5 7 170 16 169 4.21e-21 87.0
Msa0221000 MtrunA17_Chr8g0390561 31.515 165 99 5 8 170 11 163 5.85e-21 85.9
Msa0221000 MtrunA17_Chr3g0112681 32.044 181 109 5 6 184 12 180 6.60e-21 86.3
Msa0221000 MtrunA17_Chr7g0275991 32.727 165 97 5 8 170 22 174 9.06e-21 86.3
Msa0221000 MtrunA17_Chr8g0346421 33.939 165 95 5 8 170 28 180 1.81e-20 85.5
Msa0221000 MtrunA17_Chr2g0276931 30.539 167 102 5 6 170 9 163 1.85e-20 84.7
Msa0221000 MtrunA17_Chr1g0197331 29.744 195 116 7 7 191 16 199 2.17e-20 84.7
Msa0221000 MtrunA17_Chr7g0234201 31.492 181 110 5 6 184 13 181 2.84e-20 84.3
Msa0221000 MtrunA17_Chr4g0032201 29.834 181 113 5 6 184 12 180 4.32e-20 84.0
Msa0221000 MtrunA17_Chr8g0349681 28.488 172 109 5 1 170 379 538 4.64e-20 87.0
Msa0221000 MtrunA17_Chr8g0349681 28.488 172 109 5 1 170 8 167 1.07e-19 86.3
Msa0221000 MtrunA17_Chr8g0349681 27.326 172 111 5 1 170 170 329 5.96e-19 84.0
Msa0221000 MtrunA17_Chr2g0276941 30.387 181 112 5 6 184 15 183 8.65e-20 83.2
Msa0221000 MtrunA17_Chr4g0040781 36.567 134 82 3 6 137 13 145 1.27e-19 82.8
Msa0221000 MtrunA17_Chr2g0315551 31.737 167 100 5 6 170 13 167 1.97e-19 82.4
Msa0221000 MtrunA17_Chr4g0046261 29.282 181 114 5 6 184 13 181 2.48e-19 82.0
Msa0221000 MtrunA17_Chr2g0283131 30.387 181 112 5 6 184 13 181 3.12e-19 81.6
Msa0221000 MtrunA17_Chr1g0201121 29.688 192 118 6 1 184 1 183 3.27e-19 81.6
Msa0221000 MtrunA17_Chr7g0214241 29.282 181 114 5 6 184 15 183 3.74e-19 81.6
Msa0221000 MtrunA17_Chr3g0109721 30.303 165 101 4 8 170 12 164 1.09e-18 80.1
Msa0221000 MtrunA17_Chr1g0189361 30.460 174 107 5 6 177 14 175 1.69e-18 80.1
Msa0221000 MtrunA17_Chr2g0331691 29.032 186 117 5 6 185 10 186 2.59e-18 79.3
Msa0221000 MtrunA17_Chr6g0466301 28.649 185 117 5 6 184 8 183 3.34e-18 79.0
Msa0221000 MtrunA17_Chr6g0465491 28.649 185 117 5 6 184 8 183 3.34e-18 79.0
Msa0221000 MtrunA17_Chr8g0383261 29.379 177 110 4 6 176 12 179 3.89e-18 78.6
Msa0221000 MtrunA17_Chr5g0433561 29.091 165 103 4 8 170 12 164 5.46e-18 78.2
Msa0221000 MtrunA17_Chr4g0034871 27.957 186 119 4 6 185 8 184 9.20e-18 77.8
Msa0221000 MtrunA17_Chr8g0351861 31.138 167 101 5 6 170 16 170 9.42e-18 78.2
Msa0221000 MtrunA17_Chr8g0392931 29.282 181 114 5 6 184 14 182 1.04e-17 77.8
Msa0221000 MtrunA17_Chr2g0325091 30.526 190 118 5 6 193 11 188 3.98e-17 76.3
Msa0221000 MtrunA17_Chr4g0004661 28.649 185 117 5 7 185 65 240 6.41e-17 76.6
Msa0221000 MtrunA17_Chr6g0482721 33.871 124 79 3 6 127 12 134 3.86e-16 73.6
Msa0221000 MtrunA17_Chr4g0046221 72.727 55 15 0 142 196 10 64 6.03e-15 66.6
Msa0221000 MtrunA17_Chr7g0259771 27.273 187 102 3 7 180 9 174 2.89e-14 70.5
Msa0221000 MtrunA17_Chr1g0185591 28.409 176 114 5 7 180 13 178 4.90e-13 67.0
Msa0221000 MtrunA17_Chr1g0191811 29.143 175 114 5 7 180 17 182 8.49e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0221000 AT1G75840.1 85.204 196 29 0 1 196 1 196 2.91e-120 338
Msa0221000 AT4G35020.1 85.083 181 27 0 1 181 1 181 5.77e-120 338
Msa0221000 AT4G35020.2 85.083 181 27 0 1 181 1 181 5.77e-120 338
Msa0221000 AT4G35020.3 85.083 181 27 0 1 181 1 181 5.77e-120 338
Msa0221000 AT5G45970.1 81.095 201 33 1 1 196 1 201 1.61e-119 337
Msa0221000 AT1G20090.1 87.047 193 25 0 4 196 3 195 1.64e-119 337
Msa0221000 AT3G51300.1 85.955 178 25 0 1 178 1 178 1.06e-117 332
Msa0221000 AT2G17800.2 85.955 178 25 0 1 178 1 178 3.67e-117 331
Msa0221000 AT2G17800.1 85.955 178 25 0 1 178 1 178 3.67e-117 331
Msa0221000 AT4G35950.1 84.831 178 27 0 1 178 1 178 4.63e-116 328
Msa0221000 AT3G48040.1 82.123 179 32 0 2 180 4 182 7.49e-113 320
Msa0221000 AT5G62880.1 81.006 179 34 0 2 180 4 182 5.00e-112 318
Msa0221000 AT4G28950.1 76.684 193 41 2 1 191 1 191 2.76e-111 317
Msa0221000 AT2G44690.1 78.261 184 40 0 6 189 18 201 5.50e-109 311
Msa0221000 AT5G62880.2 79.070 172 32 1 2 173 4 171 2.62e-103 296
Msa0221000 AT3G48040.2 81.595 163 30 0 2 164 4 166 2.09e-102 293
Msa0221000 AT3G51290.2 85.517 145 21 0 34 178 635 779 1.01e-87 274
Msa0221000 AT4G35020.4 90.000 120 12 0 1 120 1 120 6.64e-81 237
Msa0221000 AT4G35020.5 90.000 120 12 0 1 120 1 120 6.64e-81 237
Msa0221000 AT1G43890.3 32.402 179 106 5 6 181 13 179 2.34e-26 100
Msa0221000 AT1G43890.1 32.402 179 106 5 6 181 13 179 2.34e-26 100
Msa0221000 AT1G43890.2 32.402 179 106 5 6 181 13 179 2.34e-26 100
Msa0221000 AT3G46060.2 33.533 167 98 5 6 170 15 170 2.98e-26 100
Msa0221000 AT3G46060.3 33.533 167 98 5 6 170 15 170 2.98e-26 100
Msa0221000 AT3G46060.1 33.533 167 98 5 6 170 15 170 2.98e-26 100
Msa0221000 AT4G35860.1 32.044 181 109 5 1 179 1 169 7.22e-26 99.4
Msa0221000 AT3G54840.1 33.333 177 103 5 7 180 34 198 9.07e-26 99.0
Msa0221000 AT3G53610.2 31.818 176 107 5 6 179 15 179 1.32e-25 99.0
Msa0221000 AT3G53610.3 31.818 176 107 5 6 179 15 179 1.32e-25 99.0
Msa0221000 AT3G53610.1 31.818 176 107 5 6 179 15 179 1.32e-25 99.0
Msa0221000 AT3G54840.2 33.929 168 96 5 7 171 34 189 1.59e-25 98.2
Msa0221000 AT5G59840.1 31.818 176 107 5 6 179 15 179 2.81e-25 98.2
Msa0221000 AT4G17170.1 31.977 172 103 5 1 170 1 160 3.31e-25 97.8
Msa0221000 AT3G54840.3 33.333 174 101 5 10 180 3 164 3.56e-25 96.7
Msa0221000 AT4G17160.1 30.814 172 105 5 1 170 1 160 1.11e-23 93.6
Msa0221000 AT1G18200.1 33.702 181 106 5 6 184 13 181 1.88e-23 93.6
Msa0221000 AT4G39890.1 35.294 170 94 6 4 170 8 164 4.30e-23 92.4
Msa0221000 AT1G16920.1 31.868 182 110 5 6 185 13 182 7.15e-23 91.7
Msa0221000 AT5G03530.2 29.944 177 109 5 8 181 15 179 1.19e-22 90.9
Msa0221000 AT5G03530.1 29.944 177 109 5 8 181 15 179 1.19e-22 90.9
Msa0221000 AT3G09900.1 33.523 176 104 5 6 179 15 179 1.25e-22 91.3
Msa0221000 AT1G73640.1 34.807 181 104 5 6 184 13 181 1.26e-22 91.7
Msa0221000 AT2G22290.1 36.364 165 91 5 8 170 11 163 2.01e-22 90.5
Msa0221000 AT1G02130.1 31.953 169 101 5 6 172 8 164 2.28e-22 90.1
Msa0221000 AT2G44610.1 35.758 165 92 5 8 170 11 163 5.78e-22 89.4
Msa0221000 AT5G45130.1 32.121 165 98 4 8 170 12 164 7.35e-22 88.6
Msa0221000 AT5G03520.1 33.523 176 104 5 6 179 15 179 1.13e-21 88.6
Msa0221000 AT4G19640.1 33.939 165 95 5 8 170 12 164 1.17e-21 88.2
Msa0221000 AT2G43130.1 30.220 182 113 5 6 185 12 181 1.61e-21 88.2
Msa0221000 AT5G10260.1 34.545 165 94 5 8 170 11 163 1.93e-21 87.8
Msa0221000 AT3G12160.1 35.152 165 93 5 8 170 17 169 2.28e-21 87.8
Msa0221000 AT3G11730.1 29.940 167 103 5 6 170 8 162 2.53e-21 87.4
Msa0221000 AT5G45130.2 31.902 163 97 4 10 170 22 172 2.79e-21 87.4
Msa0221000 AT1G09630.1 33.152 184 108 5 6 175 12 194 2.95e-21 87.4
Msa0221000 AT5G64990.1 35.758 165 92 5 8 170 9 161 4.48e-21 86.7
Msa0221000 AT2G31680.1 30.769 182 112 5 6 185 12 181 5.31e-21 87.0
Msa0221000 AT4G17530.1 30.481 187 111 7 6 185 8 182 1.05e-20 85.9
Msa0221000 AT5G47200.1 30.769 182 107 7 6 180 8 177 2.15e-20 84.7
Msa0221000 AT1G06400.1 32.609 184 107 6 6 187 13 181 3.24e-20 84.7
Msa0221000 AT1G22740.1 30.729 192 116 6 1 184 1 183 3.92e-20 84.3
Msa0221000 AT5G45750.1 32.335 167 99 5 6 170 13 167 6.90e-20 84.0
Msa0221000 AT5G65270.1 31.285 179 109 5 8 184 19 185 8.17e-20 84.0
Msa0221000 AT5G59150.1 30.387 181 112 5 6 184 12 180 8.32e-20 83.6
Msa0221000 AT4G18430.1 31.492 181 110 5 6 184 13 181 9.24e-20 83.6
Msa0221000 AT1G05810.1 30.220 182 113 5 6 185 12 181 9.52e-20 83.6
Msa0221000 AT4G18800.1 29.834 181 113 5 6 184 13 181 1.12e-19 83.2
Msa0221000 AT1G05810.2 31.579 171 103 5 6 174 58 216 1.23e-19 84.3
Msa0221000 AT5G47520.1 30.460 174 107 5 6 177 14 175 2.03e-19 82.8
Msa0221000 AT1G07410.1 29.834 181 113 5 6 184 12 180 2.19e-19 82.4
Msa0221000 AT3G15060.1 38.017 121 72 3 8 126 15 134 2.83e-19 82.4
Msa0221000 AT3G18820.1 29.032 186 117 5 6 185 8 184 2.97e-19 82.0
Msa0221000 AT4G19640.2 35.246 122 76 3 8 127 12 132 5.61e-19 79.7
Msa0221000 AT4G39990.1 30.723 166 101 5 8 171 19 172 6.77e-19 81.3
Msa0221000 AT3G46830.1 29.282 181 114 5 6 184 12 180 8.14e-19 81.3
Msa0221000 AT3G46830.2 29.282 181 114 5 6 184 12 180 8.14e-19 81.3
Msa0221000 AT3G07410.1 29.282 181 114 5 6 184 12 180 1.43e-18 80.5
Msa0221000 AT5G60860.1 36.585 123 75 3 6 126 13 134 1.49e-18 80.5
Msa0221000 AT5G47960.1 32.121 165 98 5 8 170 17 169 1.56e-18 80.5
Msa0221000 AT4G19640.3 35.246 122 76 3 8 127 12 132 1.66e-18 79.7
Msa0221000 AT1G28550.1 37.398 123 74 3 6 126 13 134 1.76e-18 80.1
Msa0221000 AT5G64990.2 34.302 172 92 6 8 170 9 168 2.06e-18 80.1
Msa0221000 AT2G21880.1 27.174 184 121 3 6 184 9 184 4.60e-18 79.0
Msa0221000 AT1G01200.1 32.121 165 99 5 8 170 30 183 6.55e-18 79.0
Msa0221000 AT2G33870.1 37.398 123 74 3 6 126 13 134 7.87e-18 78.6
Msa0221000 AT4G35860.2 32.090 134 78 4 47 179 2 123 1.94e-17 76.3
Msa0221000 AT1G52280.1 29.508 183 112 6 1 175 1 174 2.86e-17 76.6
Msa0221000 AT3G16100.1 28.495 186 118 5 6 185 8 184 9.88e-17 75.5
Msa0221000 AT4G09720.1 27.604 192 122 6 1 184 1 183 3.13e-16 73.9
Msa0221000 AT1G49300.2 29.240 171 106 5 6 170 8 169 6.20e-16 73.2
Msa0221000 AT1G49300.1 29.240 171 106 5 6 170 8 169 6.20e-16 73.2
Msa0221000 AT5G03520.2 33.582 134 77 4 47 179 47 169 1.40e-15 72.4
Msa0221000 AT3G09910.3 26.966 178 115 5 7 181 14 179 1.56e-15 72.0
Msa0221000 AT3G09910.1 26.966 178 115 5 7 181 14 179 1.56e-15 72.0
Msa0221000 AT3G09910.2 26.966 178 115 5 7 181 14 179 1.56e-15 72.0
Msa0221000 AT5G55080.1 32.479 117 77 2 7 122 14 129 3.53e-15 71.6
Msa0221000 AT3G09910.5 28.736 174 108 6 7 176 14 175 4.73e-15 70.5
Msa0221000 AT3G09910.4 28.736 174 108 6 7 176 14 175 4.73e-15 70.5
Msa0221000 AT4G09720.4 26.396 197 123 5 1 184 1 188 7.48e-15 70.5
Msa0221000 AT5G27540.1 25.843 178 124 3 3 180 14 183 7.48e-15 72.4
Msa0221000 AT5G27540.2 25.843 178 124 3 3 180 14 183 7.48e-15 72.4
Msa0221000 AT5G39620.1 26.943 193 128 4 7 194 6 190 1.24e-14 69.7
Msa0221000 AT3G63150.1 29.143 175 114 4 7 180 15 180 2.01e-14 71.2
Msa0221000 AT4G09720.3 25.616 203 123 5 1 184 1 194 6.25e-14 68.2
Msa0221000 AT5G59150.2 29.496 139 85 4 47 184 3 129 6.64e-13 64.3
Msa0221000 AT2G21880.4 25.749 167 111 3 23 184 1 159 4.00e-12 62.4
Msa0221000 AT2G21880.3 25.749 167 111 3 23 184 1 159 4.00e-12 62.4

Find 60 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAGCTTTCTGTTTCTTTA+AGG 0.156260 2_1:+69517630 None:intergenic
GCACAGTTAATCTTGGATTA+TGG 0.198843 2_1:-69517089 Msa0221000:CDS
TCAATACAAGCAGGAGAATT+TGG 0.242087 2_1:-69515546 Msa0221000:three_prime_UTR
TGCTGTGACATTTGATTTCA+TGG 0.261322 2_1:-69515745 Msa0221000:three_prime_UTR
CAAACATGAATACCGATTAA+AGG 0.301861 2_1:+69515776 None:intergenic
TTTAGAGATATTCTCATAAC+TGG 0.310909 2_1:+69516678 None:intergenic
TTATGAGAATATCTCTAAAA+AGG 0.315067 2_1:-69516674 Msa0221000:intron
TGTTTAGCTCCTACCAAGTT+TGG 0.332301 2_1:+69516508 None:intergenic
TTAGCTTTCTGTTTCTTTAA+GGG 0.348554 2_1:+69517631 None:intergenic
CTTTAATCGGTATTCATGTT+TGG 0.355137 2_1:-69515775 Msa0221000:exon
CACAGTTAATCTTGGATTAT+GGG 0.369623 2_1:-69517088 Msa0221000:CDS
TGTTCCTAGTTATATGTAAC+TGG 0.376841 2_1:-69515642 Msa0221000:three_prime_UTR
GGGAAGTGAAGCATTTAATA+AGG 0.384022 2_1:+69517552 None:intergenic
AGTGCAAATGTTGTGGTTGA+TGG 0.385099 2_1:-69517114 Msa0221000:CDS
TTGGAATACATTTGTTCAAA+AGG 0.391256 2_1:+69515717 None:intergenic
CAGGACAAGAGGATTATAAC+AGG 0.405871 2_1:-69516765 Msa0221000:CDS
CTTAGGCCTTTGAGCTATAG+AGG 0.408891 2_1:-69516742 Msa0221000:CDS
GATGGCAGCACAGTTAATCT+TGG 0.412892 2_1:-69517096 Msa0221000:CDS
TTGCAGATTTGAGGGAAGAT+AGG 0.415340 2_1:-69516392 Msa0221000:intron
GGAGCATAATGTCTCAGTTC+AGG 0.421519 2_1:+69516557 None:intergenic
TGTAGTGTTAAGAAAGAAAA+GGG 0.422884 2_1:+69517499 None:intergenic
TTGGTTCCCACAAGTACAAT+TGG 0.428473 2_1:+69516527 None:intergenic
TTATTGACTTGCAGATTTGA+GGG 0.432204 2_1:-69516400 Msa0221000:intron
ATTATTGACTTGCAGATTTG+AGG 0.438305 2_1:-69516401 Msa0221000:intron
GAAGAGCTGAAGAGGGCAAT+TGG 0.442300 2_1:-69516086 Msa0221000:CDS
TTCATCAAATGTGTTACTGT+TGG 0.452151 2_1:-69517321 Msa0221000:CDS
GTGTAGTGTTAAGAAAGAAA+AGG 0.474046 2_1:+69517498 None:intergenic
GTAGTTATAGCTGTAGCTCC+TGG 0.478794 2_1:+69516351 None:intergenic
GTACTTGTGGGAACCAAACT+TGG 0.487335 2_1:-69516521 Msa0221000:intron
AAGAGGATTATAACAGGCTT+AGG 0.490120 2_1:-69516759 Msa0221000:CDS
TGTGTTTGATGCTGCAATCA+AGG 0.491064 2_1:-69515857 Msa0221000:CDS
AACTGAAAGAAAGTAGAGCT+TGG 0.498823 2_1:+69515698 None:intergenic
CTTGTTTGGTGTTGTAGTAG+TGG 0.499810 2_1:+69517687 None:intergenic
ACAAGTACAATTGGCACAGT+TGG 0.513226 2_1:+69516536 None:intergenic
AGGAGCAGATGTGTTCTTGT+TGG 0.514278 2_1:-69516722 Msa0221000:CDS
ATTTAATAAGGCAAGTCCTA+TGG 0.517827 2_1:+69517564 None:intergenic
GGCTCTGCTGAGTAGTGAAA+AGG 0.521685 2_1:+69516699 None:intergenic
TTTCTGTTTCTTTAAGGGGA+AGG 0.524640 2_1:+69517636 None:intergenic
AACTGTGCCAATTGTACTTG+TGG 0.525586 2_1:-69516534 Msa0221000:CDS
TGCAATATTTCAGAATGTGA+AGG 0.527117 2_1:-69515881 Msa0221000:intron
TATCGATCGTCAATACAAGC+AGG 0.530556 2_1:-69515555 Msa0221000:three_prime_UTR
TAGCTTTCTGTTTCTTTAAG+GGG 0.533652 2_1:+69517632 None:intergenic
CTTATCACAAAATTTATCCA+TGG 0.538708 2_1:+69515615 None:intergenic
TACAAGCAGGAGAATTTGGT+TGG 0.540898 2_1:-69515542 Msa0221000:three_prime_UTR
CACACTAAAGTTGGTGCCAT+AGG 0.549969 2_1:-69517580 Msa0221000:five_prime_UTR
TACAGCTATAACTACTGCCC+AGG 0.559919 2_1:-69516343 Msa0221000:intron
ATGCAGCTCAAAGACTCAGC+AGG 0.566907 2_1:-69516045 Msa0221000:intron
CTTGGATTATGGGACACTGC+TGG 0.569014 2_1:-69517078 Msa0221000:intron
GGCAGGGTGAAGAGCTGAAG+AGG 0.570105 2_1:-69516094 Msa0221000:intron
CAGTATTTGATTGATCATCC+AGG 0.575063 2_1:-69516369 Msa0221000:CDS
CAGTGATGAAAACAACAAAA+CGG 0.589997 2_1:-69517407 Msa0221000:five_prime_UTR
ACTGTGCCAATTGTACTTGT+GGG 0.592943 2_1:-69516533 Msa0221000:CDS
ATATCCAGTTACATATAACT+AGG 0.598387 2_1:+69515638 None:intergenic
TATATGTAACTGGATATCCA+TGG 0.598619 2_1:-69515632 Msa0221000:three_prime_UTR
CACAAGCAATACATTCCCTA+CGG 0.607441 2_1:-69517259 Msa0221000:intron
ACTGTTGGAGATGGTGCTGT+TGG 0.608648 2_1:-69517306 Msa0221000:CDS
TAACTTCAGTGCAAATGTTG+TGG 0.625689 2_1:-69517121 Msa0221000:CDS
TCTGCTCCTCTATAGCTCAA+AGG 0.639148 2_1:+69516736 None:intergenic
AAATGTGTTACTGTTGGAGA+TGG 0.644647 2_1:-69517315 Msa0221000:CDS
GCAGGGTGAAGAGCTGAAGA+GGG 0.664533 2_1:-69516093 Msa0221000:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTTTTCATAAAAATTC+AGG - chr2_1:69516442-69516461 Msa0221000:intron 10.0%
!! TAAACATATATAACTTAATT+GGG - chr2_1:69516735-69516754 Msa0221000:CDS 10.0%
!! GTAGTAATTTAATAATTAAT+TGG - chr2_1:69516793-69516812 Msa0221000:intron 10.0%
!! ACAAATAATGTAAAATTTAT+GGG - chr2_1:69517111-69517130 Msa0221000:CDS 10.0%
!!! TAATTTTTTCATAAAAATTC+AGG - chr2_1:69516442-69516461 Msa0221000:intron 10.0%
!! TAAACATATATAACTTAATT+GGG - chr2_1:69516735-69516754 Msa0221000:CDS 10.0%
!! GTAGTAATTTAATAATTAAT+TGG - chr2_1:69516793-69516812 Msa0221000:intron 10.0%
!! ACAAATAATGTAAAATTTAT+GGG - chr2_1:69517111-69517130 Msa0221000:CDS 10.0%
!!! ATTTGAATTTTGAATTGAAA+AGG + chr2_1:69515702-69515721 None:intergenic 15.0%
!!! TAACAAATTTAGTTTATGTT+TGG - chr2_1:69516022-69516041 Msa0221000:intron 15.0%
!! ACAAATTATAAATCGTAAAA+CGG + chr2_1:69516204-69516223 None:intergenic 15.0%
!! ATGAAAAAATTAGTAAATCT+TGG + chr2_1:69516434-69516453 None:intergenic 15.0%
!! CTAAACATATATAACTTAAT+TGG - chr2_1:69516734-69516753 Msa0221000:CDS 15.0%
!! TAATTATTAAATTACTACCA+AGG + chr2_1:69516791-69516810 None:intergenic 15.0%
!! CACAAATAATGTAAAATTTA+TGG - chr2_1:69517110-69517129 Msa0221000:CDS 15.0%
!! TTAACATAATCAGAAATTAT+TGG - chr2_1:69517249-69517268 Msa0221000:intron 15.0%
!!! ATTTGAATTTTGAATTGAAA+AGG + chr2_1:69515702-69515721 None:intergenic 15.0%
!!! TAACAAATTTAGTTTATGTT+TGG - chr2_1:69516022-69516041 Msa0221000:intron 15.0%
!! ACAAATTATAAATCGTAAAA+CGG + chr2_1:69516204-69516223 None:intergenic 15.0%
!! ATGAAAAAATTAGTAAATCT+TGG + chr2_1:69516434-69516453 None:intergenic 15.0%
!! CTAAACATATATAACTTAAT+TGG - chr2_1:69516734-69516753 Msa0221000:CDS 15.0%
!! TAATTATTAAATTACTACCA+AGG + chr2_1:69516791-69516810 None:intergenic 15.0%
!! CACAAATAATGTAAAATTTA+TGG - chr2_1:69517110-69517129 Msa0221000:CDS 15.0%
!! TTAACATAATCAGAAATTAT+TGG - chr2_1:69517249-69517268 Msa0221000:intron 15.0%
!!! TTATGAGAATATCTCTAAAA+AGG - chr2_1:69516552-69516571 Msa0221000:CDS 20.0%
!!! TTCACTTGTGAATTTTTATA+TGG - chr2_1:69516595-69516614 Msa0221000:intron 20.0%
!! ATTCCAAATAGTATTTAAGT+TGG - chr2_1:69516991-69517010 Msa0221000:intron 20.0%
!! CATATATAGTTATATACTCA+TGG - chr2_1:69517221-69517240 Msa0221000:intron 20.0%
!!! TTTCTTTTTTTCTTTTGCTT+AGG + chr2_1:69517409-69517428 None:intergenic 20.0%
!!! ATCATGTATTTTCCTTTAAT+CGG - chr2_1:69517438-69517457 Msa0221000:five_prime_UTR 20.0%
!!! TTCTTGTATTTTTTTGTTGA+TGG - chr2_1:69517551-69517570 Msa0221000:five_prime_UTR 20.0%
!!! TTATGAGAATATCTCTAAAA+AGG - chr2_1:69516552-69516571 Msa0221000:CDS 20.0%
!!! TTCACTTGTGAATTTTTATA+TGG - chr2_1:69516595-69516614 Msa0221000:intron 20.0%
!! ATTCCAAATAGTATTTAAGT+TGG - chr2_1:69516991-69517010 Msa0221000:intron 20.0%
!! CATATATAGTTATATACTCA+TGG - chr2_1:69517221-69517240 Msa0221000:intron 20.0%
!!! TTTCTTTTTTTCTTTTGCTT+AGG + chr2_1:69517409-69517428 None:intergenic 20.0%
!!! ATCATGTATTTTCCTTTAAT+CGG - chr2_1:69517438-69517457 Msa0221000:five_prime_UTR 20.0%
!!! TTCTTGTATTTTTTTGTTGA+TGG - chr2_1:69517551-69517570 Msa0221000:five_prime_UTR 20.0%
!!! AGTTTTTAGATCAGCAAAAA+AGG + chr2_1:69515566-69515585 None:intergenic 25.0%
!! TTAGCTTTCTGTTTCTTTAA+GGG + chr2_1:69515598-69515617 None:intergenic 25.0%
!!! TTTAGCTTTCTGTTTCTTTA+AGG + chr2_1:69515599-69515618 None:intergenic 25.0%
!! AATTTTGAATTGAAAAGGAG+GGG + chr2_1:69515697-69515716 None:intergenic 25.0%
!! GAATTTTGAATTGAAAAGGA+GGG + chr2_1:69515698-69515717 None:intergenic 25.0%
!! TGAATTTTGAATTGAAAAGG+AGG + chr2_1:69515699-69515718 None:intergenic 25.0%
! TGTAGTGTTAAGAAAGAAAA+GGG + chr2_1:69515730-69515749 None:intergenic 25.0%
!!! GTTCAAATATTTTCTAGTGA+AGG - chr2_1:69515778-69515797 Msa0221000:exon 25.0%
!!! CTTGTTCTTGTTTTTTTTGA+AGG - chr2_1:69516060-69516079 Msa0221000:CDS 25.0%
! TAAGTAGCAAAGACATTTAA+CGG - chr2_1:69516237-69516256 Msa0221000:intron 25.0%
! TTTAGAGATATTCTCATAAC+TGG + chr2_1:69516551-69516570 None:intergenic 25.0%
! TCATACAAATTTGTAGGAAT+TGG - chr2_1:69516910-69516929 Msa0221000:intron 25.0%
! ATGCCAACTTAAATACTATT+TGG + chr2_1:69516997-69517016 None:intergenic 25.0%
! ATAATGTAAAATTTATGGGC+AGG - chr2_1:69517115-69517134 Msa0221000:CDS 25.0%
! TAATGTAAAATTTATGGGCA+GGG - chr2_1:69517116-69517135 Msa0221000:CDS 25.0%
! TTGGAATACATTTGTTCAAA+AGG + chr2_1:69517512-69517531 None:intergenic 25.0%
! ATATCCAGTTACATATAACT+AGG + chr2_1:69517591-69517610 None:intergenic 25.0%
! CTTATCACAAAATTTATCCA+TGG + chr2_1:69517614-69517633 None:intergenic 25.0%
!!! AGTTTTTAGATCAGCAAAAA+AGG + chr2_1:69515566-69515585 None:intergenic 25.0%
!! TTAGCTTTCTGTTTCTTTAA+GGG + chr2_1:69515598-69515617 None:intergenic 25.0%
!!! TTTAGCTTTCTGTTTCTTTA+AGG + chr2_1:69515599-69515618 None:intergenic 25.0%
!! AATTTTGAATTGAAAAGGAG+GGG + chr2_1:69515697-69515716 None:intergenic 25.0%
!! GAATTTTGAATTGAAAAGGA+GGG + chr2_1:69515698-69515717 None:intergenic 25.0%
!! TGAATTTTGAATTGAAAAGG+AGG + chr2_1:69515699-69515718 None:intergenic 25.0%
! TGTAGTGTTAAGAAAGAAAA+GGG + chr2_1:69515730-69515749 None:intergenic 25.0%
!!! GTTCAAATATTTTCTAGTGA+AGG - chr2_1:69515778-69515797 Msa0221000:exon 25.0%
!!! CTTGTTCTTGTTTTTTTTGA+AGG - chr2_1:69516060-69516079 Msa0221000:CDS 25.0%
! TAAGTAGCAAAGACATTTAA+CGG - chr2_1:69516237-69516256 Msa0221000:intron 25.0%
! TTTAGAGATATTCTCATAAC+TGG + chr2_1:69516551-69516570 None:intergenic 25.0%
! TCATACAAATTTGTAGGAAT+TGG - chr2_1:69516910-69516929 Msa0221000:intron 25.0%
! ATGCCAACTTAAATACTATT+TGG + chr2_1:69516997-69517016 None:intergenic 25.0%
! ATAATGTAAAATTTATGGGC+AGG - chr2_1:69517115-69517134 Msa0221000:CDS 25.0%
! TAATGTAAAATTTATGGGCA+GGG - chr2_1:69517116-69517135 Msa0221000:CDS 25.0%
! TTGGAATACATTTGTTCAAA+AGG + chr2_1:69517512-69517531 None:intergenic 25.0%
! ATATCCAGTTACATATAACT+AGG + chr2_1:69517591-69517610 None:intergenic 25.0%
! CTTATCACAAAATTTATCCA+TGG + chr2_1:69517614-69517633 None:intergenic 25.0%
! TAGCTTTCTGTTTCTTTAAG+GGG + chr2_1:69515597-69515616 None:intergenic 30.0%
AAAAAGAGTCACACTAAAGT+TGG - chr2_1:69515637-69515656 Msa0221000:three_prime_UTR 30.0%
ATTTAATAAGGCAAGTCCTA+TGG + chr2_1:69515665-69515684 None:intergenic 30.0%
GTGTAGTGTTAAGAAAGAAA+AGG + chr2_1:69515731-69515750 None:intergenic 30.0%
CAGTGATGAAAACAACAAAA+CGG - chr2_1:69515819-69515838 Msa0221000:CDS 30.0%
!! CTTTGCTTTGAAAGAAAAAC+AGG - chr2_1:69515851-69515870 Msa0221000:CDS 30.0%
! TTCATCAAATGTGTTACTGT+TGG - chr2_1:69515905-69515924 Msa0221000:intron 30.0%
CTGAAGTTATCAAAAACTGT+AGG + chr2_1:69516094-69516113 None:intergenic 30.0%
CACAGTTAATCTTGGATTAT+GGG - chr2_1:69516138-69516157 Msa0221000:intron 30.0%
! TGCTGGTAAGAATTTTCTTT+AGG - chr2_1:69516165-69516184 Msa0221000:intron 30.0%
! TGGTAAGAATTTTCTTTAGG+TGG - chr2_1:69516168-69516187 Msa0221000:intron 30.0%
CACAAAAACAACATTCTATC+TGG + chr2_1:69516334-69516353 None:intergenic 30.0%
ACTATTAATTACACACAGAG+AGG + chr2_1:69516401-69516420 None:intergenic 30.0%
TCATAAAAATTCAGGACAAG+AGG - chr2_1:69516450-69516469 Msa0221000:intron 30.0%
TTACGATAAAAGTTTAGCCT+TGG - chr2_1:69516771-69516790 Msa0221000:intron 30.0%
!! ATTATTGACTTGCAGATTTG+AGG - chr2_1:69516825-69516844 Msa0221000:intron 30.0%
!! TTATTGACTTGCAGATTTGA+GGG - chr2_1:69516826-69516845 Msa0221000:intron 30.0%
CAAATTTGTATGACTTACCT+GGG + chr2_1:69516903-69516922 None:intergenic 30.0%
ACAAATTTGTATGACTTACC+TGG + chr2_1:69516904-69516923 None:intergenic 30.0%
GGTAAGTCATACAAATTTGT+AGG - chr2_1:69516904-69516923 Msa0221000:intron 30.0%
TTAGTACAAAGCTAATAGTG+TGG + chr2_1:69517311-69517330 None:intergenic 30.0%
TGCAATATTTCAGAATGTGA+AGG - chr2_1:69517345-69517364 Msa0221000:exon 30.0%
!!! CTTTTTTTCTTTTGCTTAGG+AGG + chr2_1:69517406-69517425 None:intergenic 30.0%
! CAAACATGAATACCGATTAA+AGG + chr2_1:69517453-69517472 None:intergenic 30.0%
CTTTAATCGGTATTCATGTT+TGG - chr2_1:69517451-69517470 Msa0221000:five_prime_UTR 30.0%
TGTTCCTAGTTATATGTAAC+TGG - chr2_1:69517584-69517603 Msa0221000:five_prime_UTR 30.0%
TATATGTAACTGGATATCCA+TGG - chr2_1:69517594-69517613 Msa0221000:five_prime_UTR 30.0%
! TAGCTTTCTGTTTCTTTAAG+GGG + chr2_1:69515597-69515616 None:intergenic 30.0%
AAAAAGAGTCACACTAAAGT+TGG - chr2_1:69515637-69515656 Msa0221000:three_prime_UTR 30.0%
ATTTAATAAGGCAAGTCCTA+TGG + chr2_1:69515665-69515684 None:intergenic 30.0%
GTGTAGTGTTAAGAAAGAAA+AGG + chr2_1:69515731-69515750 None:intergenic 30.0%
CAGTGATGAAAACAACAAAA+CGG - chr2_1:69515819-69515838 Msa0221000:CDS 30.0%
!! CTTTGCTTTGAAAGAAAAAC+AGG - chr2_1:69515851-69515870 Msa0221000:CDS 30.0%
! TTCATCAAATGTGTTACTGT+TGG - chr2_1:69515905-69515924 Msa0221000:intron 30.0%
CTGAAGTTATCAAAAACTGT+AGG + chr2_1:69516094-69516113 None:intergenic 30.0%
CACAGTTAATCTTGGATTAT+GGG - chr2_1:69516138-69516157 Msa0221000:intron 30.0%
! TGCTGGTAAGAATTTTCTTT+AGG - chr2_1:69516165-69516184 Msa0221000:intron 30.0%
! TGGTAAGAATTTTCTTTAGG+TGG - chr2_1:69516168-69516187 Msa0221000:intron 30.0%
CACAAAAACAACATTCTATC+TGG + chr2_1:69516334-69516353 None:intergenic 30.0%
ACTATTAATTACACACAGAG+AGG + chr2_1:69516401-69516420 None:intergenic 30.0%
TCATAAAAATTCAGGACAAG+AGG - chr2_1:69516450-69516469 Msa0221000:intron 30.0%
TTACGATAAAAGTTTAGCCT+TGG - chr2_1:69516771-69516790 Msa0221000:intron 30.0%
!! ATTATTGACTTGCAGATTTG+AGG - chr2_1:69516825-69516844 Msa0221000:intron 30.0%
!! TTATTGACTTGCAGATTTGA+GGG - chr2_1:69516826-69516845 Msa0221000:intron 30.0%
CAAATTTGTATGACTTACCT+GGG + chr2_1:69516903-69516922 None:intergenic 30.0%
ACAAATTTGTATGACTTACC+TGG + chr2_1:69516904-69516923 None:intergenic 30.0%
GGTAAGTCATACAAATTTGT+AGG - chr2_1:69516904-69516923 Msa0221000:intron 30.0%
TTAGTACAAAGCTAATAGTG+TGG + chr2_1:69517311-69517330 None:intergenic 30.0%
TGCAATATTTCAGAATGTGA+AGG - chr2_1:69517345-69517364 Msa0221000:exon 30.0%
!!! CTTTTTTTCTTTTGCTTAGG+AGG + chr2_1:69517406-69517425 None:intergenic 30.0%
! CAAACATGAATACCGATTAA+AGG + chr2_1:69517453-69517472 None:intergenic 30.0%
CTTTAATCGGTATTCATGTT+TGG - chr2_1:69517451-69517470 Msa0221000:five_prime_UTR 30.0%
TGTTCCTAGTTATATGTAAC+TGG - chr2_1:69517584-69517603 Msa0221000:five_prime_UTR 30.0%
TATATGTAACTGGATATCCA+TGG - chr2_1:69517594-69517613 Msa0221000:five_prime_UTR 30.0%
! TCAGCAAAAAAGGACTTGTT+TGG + chr2_1:69515556-69515575 None:intergenic 35.0%
! TTTCTGTTTCTTTAAGGGGA+AGG + chr2_1:69515593-69515612 None:intergenic 35.0%
! GGGAAGTGAAGCATTTAATA+AGG + chr2_1:69515677-69515696 None:intergenic 35.0%
!! TGCTTTGAAAGAAAAACAGG+AGG - chr2_1:69515854-69515873 Msa0221000:CDS 35.0%
! AAATGTGTTACTGTTGGAGA+TGG - chr2_1:69515911-69515930 Msa0221000:intron 35.0%
! TGTGTTGTTATCATACCGTA+GGG + chr2_1:69515985-69516004 None:intergenic 35.0%
! ATGTGTTGTTATCATACCGT+AGG + chr2_1:69515986-69516005 None:intergenic 35.0%
TAACTTCAGTGCAAATGTTG+TGG - chr2_1:69516105-69516124 Msa0221000:intron 35.0%
GCACAGTTAATCTTGGATTA+TGG - chr2_1:69516137-69516156 Msa0221000:intron 35.0%
AAGAGGATTATAACAGGCTT+AGG - chr2_1:69516467-69516486 Msa0221000:intron 35.0%
! CACTAGCTAATTTTATGCAG+TGG - chr2_1:69516643-69516662 Msa0221000:intron 35.0%
CAGTATTTGATTGATCATCC+AGG - chr2_1:69516857-69516876 Msa0221000:intron 35.0%
! CACGCTATCAAGGTTTTTAT+AGG - chr2_1:69517075-69517094 Msa0221000:intron 35.0%
! TGCTGTGACATTTGATTTCA+TGG - chr2_1:69517481-69517500 Msa0221000:five_prime_UTR 35.0%
AACTGAAAGAAAGTAGAGCT+TGG + chr2_1:69517531-69517550 None:intergenic 35.0%
TCAATACAAGCAGGAGAATT+TGG - chr2_1:69517680-69517699 Msa0221000:five_prime_UTR 35.0%
! TCAGCAAAAAAGGACTTGTT+TGG + chr2_1:69515556-69515575 None:intergenic 35.0%
! TTTCTGTTTCTTTAAGGGGA+AGG + chr2_1:69515593-69515612 None:intergenic 35.0%
! GGGAAGTGAAGCATTTAATA+AGG + chr2_1:69515677-69515696 None:intergenic 35.0%
!! TGCTTTGAAAGAAAAACAGG+AGG - chr2_1:69515854-69515873 Msa0221000:CDS 35.0%
! AAATGTGTTACTGTTGGAGA+TGG - chr2_1:69515911-69515930 Msa0221000:intron 35.0%
! TGTGTTGTTATCATACCGTA+GGG + chr2_1:69515985-69516004 None:intergenic 35.0%
! ATGTGTTGTTATCATACCGT+AGG + chr2_1:69515986-69516005 None:intergenic 35.0%
TAACTTCAGTGCAAATGTTG+TGG - chr2_1:69516105-69516124 Msa0221000:intron 35.0%
GCACAGTTAATCTTGGATTA+TGG - chr2_1:69516137-69516156 Msa0221000:intron 35.0%
AAGAGGATTATAACAGGCTT+AGG - chr2_1:69516467-69516486 Msa0221000:intron 35.0%
! CACTAGCTAATTTTATGCAG+TGG - chr2_1:69516643-69516662 Msa0221000:intron 35.0%
CAGTATTTGATTGATCATCC+AGG - chr2_1:69516857-69516876 Msa0221000:intron 35.0%
! CACGCTATCAAGGTTTTTAT+AGG - chr2_1:69517075-69517094 Msa0221000:intron 35.0%
! TGCTGTGACATTTGATTTCA+TGG - chr2_1:69517481-69517500 Msa0221000:five_prime_UTR 35.0%
AACTGAAAGAAAGTAGAGCT+TGG + chr2_1:69517531-69517550 None:intergenic 35.0%
TCAATACAAGCAGGAGAATT+TGG - chr2_1:69517680-69517699 Msa0221000:five_prime_UTR 35.0%
CACAAGCAATACATTCCCTA+CGG - chr2_1:69515967-69515986 Msa0221000:intron 40.0%
AGTGCAAATGTTGTGGTTGA+TGG - chr2_1:69516112-69516131 Msa0221000:intron 40.0%
CAGGACAAGAGGATTATAAC+AGG - chr2_1:69516461-69516480 Msa0221000:intron 40.0%
ACAAGTACAATTGGCACAGT+TGG + chr2_1:69516693-69516712 None:intergenic 40.0%
AACTGTGCCAATTGTACTTG+TGG - chr2_1:69516692-69516711 Msa0221000:CDS 40.0%
ACTGTGCCAATTGTACTTGT+GGG - chr2_1:69516693-69516712 Msa0221000:CDS 40.0%
TTGGTTCCCACAAGTACAAT+TGG + chr2_1:69516702-69516721 None:intergenic 40.0%
! TGTTTAGCTCCTACCAAGTT+TGG + chr2_1:69516721-69516740 None:intergenic 40.0%
TTGCAGATTTGAGGGAAGAT+AGG - chr2_1:69516834-69516853 Msa0221000:intron 40.0%
AATTGAGAGATCTCAGTCGT+TGG + chr2_1:69517040-69517059 None:intergenic 40.0%
ATTGCGTTAACACGCTATCA+AGG - chr2_1:69517065-69517084 Msa0221000:intron 40.0%
TGTGTTTGATGCTGCAATCA+AGG - chr2_1:69517369-69517388 Msa0221000:five_prime_UTR 40.0%
TATCGATCGTCAATACAAGC+AGG - chr2_1:69517671-69517690 Msa0221000:five_prime_UTR 40.0%
TACAAGCAGGAGAATTTGGT+TGG - chr2_1:69517684-69517703 Msa0221000:five_prime_UTR 40.0%
CACAAGCAATACATTCCCTA+CGG - chr2_1:69515967-69515986 Msa0221000:intron 40.0%
AGTGCAAATGTTGTGGTTGA+TGG - chr2_1:69516112-69516131 Msa0221000:intron 40.0%
CAGGACAAGAGGATTATAAC+AGG - chr2_1:69516461-69516480 Msa0221000:intron 40.0%
ACAAGTACAATTGGCACAGT+TGG + chr2_1:69516693-69516712 None:intergenic 40.0%
AACTGTGCCAATTGTACTTG+TGG - chr2_1:69516692-69516711 Msa0221000:CDS 40.0%
ACTGTGCCAATTGTACTTGT+GGG - chr2_1:69516693-69516712 Msa0221000:CDS 40.0%
TTGGTTCCCACAAGTACAAT+TGG + chr2_1:69516702-69516721 None:intergenic 40.0%
! TGTTTAGCTCCTACCAAGTT+TGG + chr2_1:69516721-69516740 None:intergenic 40.0%
TTGCAGATTTGAGGGAAGAT+AGG - chr2_1:69516834-69516853 Msa0221000:intron 40.0%
AATTGAGAGATCTCAGTCGT+TGG + chr2_1:69517040-69517059 None:intergenic 40.0%
ATTGCGTTAACACGCTATCA+AGG - chr2_1:69517065-69517084 Msa0221000:intron 40.0%
TGTGTTTGATGCTGCAATCA+AGG - chr2_1:69517369-69517388 Msa0221000:five_prime_UTR 40.0%
TATCGATCGTCAATACAAGC+AGG - chr2_1:69517671-69517690 Msa0221000:five_prime_UTR 40.0%
!! CACACTAAAGTTGGTGCCAT+AGG - chr2_1:69515646-69515665 Msa0221000:three_prime_UTR 45.0%
GATGGCAGCACAGTTAATCT+TGG - chr2_1:69516130-69516149 Msa0221000:intron 45.0%
CTTAGGCCTTTGAGCTATAG+AGG - chr2_1:69516484-69516503 Msa0221000:intron 45.0%
TCTGCTCCTCTATAGCTCAA+AGG + chr2_1:69516493-69516512 None:intergenic 45.0%
AGGAGCAGATGTGTTCTTGT+TGG - chr2_1:69516504-69516523 Msa0221000:intron 45.0%
GGAGCATAATGTCTCAGTTC+AGG + chr2_1:69516672-69516691 None:intergenic 45.0%
GTACTTGTGGGAACCAAACT+TGG - chr2_1:69516705-69516724 Msa0221000:CDS 45.0%
TTGTGGGAACCAAACTTGGT+AGG - chr2_1:69516709-69516728 Msa0221000:CDS 45.0%
GTAGTTATAGCTGTAGCTCC+TGG + chr2_1:69516878-69516897 None:intergenic 45.0%
TACAGCTATAACTACTGCCC+AGG - chr2_1:69516883-69516902 Msa0221000:intron 45.0%
CAGATTTCAGCCGCTAATGA+TGG - chr2_1:69516955-69516974 Msa0221000:intron 45.0%
AGTCGTCTAGCCATCATTAG+CGG + chr2_1:69516968-69516987 None:intergenic 45.0%
!! CACACTAAAGTTGGTGCCAT+AGG - chr2_1:69515646-69515665 Msa0221000:three_prime_UTR 45.0%
GATGGCAGCACAGTTAATCT+TGG - chr2_1:69516130-69516149 Msa0221000:intron 45.0%
CTTAGGCCTTTGAGCTATAG+AGG - chr2_1:69516484-69516503 Msa0221000:intron 45.0%
TCTGCTCCTCTATAGCTCAA+AGG + chr2_1:69516493-69516512 None:intergenic 45.0%
AGGAGCAGATGTGTTCTTGT+TGG - chr2_1:69516504-69516523 Msa0221000:intron 45.0%
GGAGCATAATGTCTCAGTTC+AGG + chr2_1:69516672-69516691 None:intergenic 45.0%
GTACTTGTGGGAACCAAACT+TGG - chr2_1:69516705-69516724 Msa0221000:CDS 45.0%
TTGTGGGAACCAAACTTGGT+AGG - chr2_1:69516709-69516728 Msa0221000:CDS 45.0%
GTAGTTATAGCTGTAGCTCC+TGG + chr2_1:69516878-69516897 None:intergenic 45.0%
TACAGCTATAACTACTGCCC+AGG - chr2_1:69516883-69516902 Msa0221000:intron 45.0%
CAGATTTCAGCCGCTAATGA+TGG - chr2_1:69516955-69516974 Msa0221000:intron 45.0%
AGTCGTCTAGCCATCATTAG+CGG + chr2_1:69516968-69516987 None:intergenic 45.0%
!! ACTGTTGGAGATGGTGCTGT+TGG - chr2_1:69515920-69515939 Msa0221000:intron 50.0%
CTTGGATTATGGGACACTGC+TGG - chr2_1:69516148-69516167 Msa0221000:intron 50.0%
GGCTCTGCTGAGTAGTGAAA+AGG + chr2_1:69516530-69516549 None:intergenic 50.0%
GAAGAGCTGAAGAGGGCAAT+TGG - chr2_1:69517140-69517159 Msa0221000:CDS 50.0%
ATGCAGCTCAAAGACTCAGC+AGG - chr2_1:69517181-69517200 Msa0221000:intron 50.0%
!! ACTGTTGGAGATGGTGCTGT+TGG - chr2_1:69515920-69515939 Msa0221000:intron 50.0%
CTTGGATTATGGGACACTGC+TGG - chr2_1:69516148-69516167 Msa0221000:intron 50.0%
GGCTCTGCTGAGTAGTGAAA+AGG + chr2_1:69516530-69516549 None:intergenic 50.0%
GAAGAGCTGAAGAGGGCAAT+TGG - chr2_1:69517140-69517159 Msa0221000:CDS 50.0%
ATGCAGCTCAAAGACTCAGC+AGG - chr2_1:69517181-69517200 Msa0221000:intron 50.0%
GCAGGGTGAAGAGCTGAAGA+GGG - chr2_1:69517133-69517152 Msa0221000:CDS 55.0%
GCAGGGTGAAGAGCTGAAGA+GGG - chr2_1:69517133-69517152 Msa0221000:CDS 55.0%
GGCAGGGTGAAGAGCTGAAG+AGG - chr2_1:69517132-69517151 Msa0221000:CDS 60.0%
GGCAGGGTGAAGAGCTGAAG+AGG - chr2_1:69517132-69517151 Msa0221000:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 69515542 69517706 69515542 ID=Msa0221000;Name=Msa0221000
chr2_1 mRNA 69515542 69517706 69515542 ID=Msa0221000-mRNA-1;Parent=Msa0221000;Name=Msa0221000-mRNA-1;_AED=0.12;_eAED=0.12;_QI=348|1|1|1|1|1|7|250|196
chr2_1 exon 69515542 69515890 69515542 ID=Msa0221000-mRNA-1:exon:19715;Parent=Msa0221000-mRNA-1
chr2_1 exon 69516046 69516111 69516046 ID=Msa0221000-mRNA-1:exon:19714;Parent=Msa0221000-mRNA-1
chr2_1 exon 69516344 69516408 69516344 ID=Msa0221000-mRNA-1:exon:19713;Parent=Msa0221000-mRNA-1
chr2_1 exon 69516522 69516585 69516522 ID=Msa0221000-mRNA-1:exon:19712;Parent=Msa0221000-mRNA-1
chr2_1 exon 69516675 69516784 69516675 ID=Msa0221000-mRNA-1:exon:19711;Parent=Msa0221000-mRNA-1
chr2_1 exon 69517079 69517166 69517079 ID=Msa0221000-mRNA-1:exon:19710;Parent=Msa0221000-mRNA-1
chr2_1 exon 69517260 69517706 69517260 ID=Msa0221000-mRNA-1:exon:19709;Parent=Msa0221000-mRNA-1
chr2_1 five_prime_UTR 69517359 69517706 69517359 ID=Msa0221000-mRNA-1:five_prime_utr;Parent=Msa0221000-mRNA-1
chr2_1 CDS 69517260 69517358 69517260 ID=Msa0221000-mRNA-1:cds;Parent=Msa0221000-mRNA-1
chr2_1 CDS 69517079 69517166 69517079 ID=Msa0221000-mRNA-1:cds;Parent=Msa0221000-mRNA-1
chr2_1 CDS 69516675 69516784 69516675 ID=Msa0221000-mRNA-1:cds;Parent=Msa0221000-mRNA-1
chr2_1 CDS 69516522 69516585 69516522 ID=Msa0221000-mRNA-1:cds;Parent=Msa0221000-mRNA-1
chr2_1 CDS 69516344 69516408 69516344 ID=Msa0221000-mRNA-1:cds;Parent=Msa0221000-mRNA-1
chr2_1 CDS 69516046 69516111 69516046 ID=Msa0221000-mRNA-1:cds;Parent=Msa0221000-mRNA-1
chr2_1 CDS 69515792 69515890 69515792 ID=Msa0221000-mRNA-1:cds;Parent=Msa0221000-mRNA-1
chr2_1 three_prime_UTR 69515542 69515791 69515542 ID=Msa0221000-mRNA-1:three_prime_utr;Parent=Msa0221000-mRNA-1
Gene Sequence

>Msa0221000

ATGAGTACAGCAAGATTCATCAAATGTGTTACTGTTGGAGATGGTGCTGTTGGAAAGACTTGTATGCTTATCTCTTACACAAGCAATACATTCCCTACGGATTATGTGCCTACAGTTTTTGATAACTTCAGTGCAAATGTTGTGGTTGATGGCAGCACAGTTAATCTTGGATTATGGGACACTGCTGGACAAGAGGATTATAACAGGCTTAGGCCTTTGAGCTATAGAGGAGCAGATGTGTTCTTGTTGGCCTTTTCACTACTCAGCAGAGCCAGTTATGAGAATATCTCTAAAAAGTGGATTCCTGAACTGAGACATTATGCTCCAACTGTGCCAATTGTACTTGTGGGAACCAAACTTGATTTGAGGGAAGATAGGCAGTATTTGATTGATCATCCAGGAGCTACAGCTATAACTACTGCCCAGGGTGAAGAGCTGAAGAGGGCAATTGGTGCAGCAGTGTACTTAGAATGCAGCTCAAAGACTCAGCAGAATGTGAAGGCTGTGTTTGATGCTGCAATCAAGGTTGTTTTGCAGCCTCCTAAGCAAAAGAAAAAAAGAAAGAAGAACAGATCATGTATTTTCCTTTAA
Protein sequence

>Msa0221000

MSTARFIKCVTVGDGAVGKTCMLISYTSNTFPTDYVPTVFDNFSANVVVDGSTVNLGLWDTAGQEDYNRLRPLSYRGADVFLLAFSLLSRASYENISKKWIPELRHYAPTVPIVLVGTKLDLREDRQYLIDHPGATAITTAQGEELKRAIGAAVYLECSSKTQQNVKAVFDAAIKVVLQPPKQKKKRKKNRSCIFL*