AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0631990


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0631990 MtrunA17_Chr4g0009821 94.667 75 4 0 1 75 1 75 1.31e-45 140
Msa0631990 MtrunA17_Chr4g0009811 82.667 75 13 0 1 75 1 75 1.70e-39 124
Msa0631990 MtrunA17_Chr7g0238571 56.522 69 27 2 8 75 12 78 1.71e-13 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 35 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCAAATGTTCTTAGATTT+TGG 0.126318 4_3:-15538231 Msa0631990:three_prime_UTR
AACCACAGAAACCGTGATTT+TGG 0.184588 4_3:-15538516 Msa0631990:CDS
CCATTTCAGCACCTTCAAAT+TGG 0.222108 4_3:+15538474 None:intergenic
TGCAAAGAACAAATTTATAT+TGG 0.266509 4_3:+15539340 None:intergenic
TAATATATTCTATTTATAAT+AGG 0.305439 4_3:+15538120 None:intergenic
GGTTTCTGTGGTTTCGGCCT+CGG 0.316189 4_3:+15538526 None:intergenic
AATCACGGTTTCTGTGGTTT+CGG 0.340586 4_3:+15538520 None:intergenic
ATACACACAATTACACATAT+TGG 0.348552 4_3:+15538335 None:intergenic
AGCACCTTCAAATTGGATCT+TGG 0.377145 4_3:+15538481 None:intergenic
GCACTTCATTTGCAGTTGCA+AGG 0.406733 4_3:+15538398 None:intergenic
CCAATTTGAAGGTGCTGAAA+TGG 0.415709 4_3:-15538474 Msa0631990:CDS
AGCTATGATCGCTTCGTGTT+TGG 0.420712 4_3:-15538193 Msa0631990:three_prime_UTR
GCCTCTTGAAGCAGAGATAG+TGG 0.443076 4_3:-15538360 Msa0631990:three_prime_UTR
AAGAACAAATTTATATTGGT+TGG 0.448696 4_3:+15539344 None:intergenic
AGAACATTTGAATTTGATGA+AGG 0.454645 4_3:+15538241 None:intergenic
CACTTCATTTGCAGTTGCAA+GGG 0.477731 4_3:+15538399 None:intergenic
TTGGTTCATCAATATCAAGT+TGG 0.502926 4_3:-15538073 Msa0631990:three_prime_UTR
AGAATAATATGAAGTGGTCA+TGG 0.517932 4_3:-15538270 Msa0631990:three_prime_UTR
CAATTTGAAGGTGCTGAAAT+GGG 0.519160 4_3:-15538473 Msa0631990:CDS
CCTTCAAATTGGATCTTGGC+CGG 0.520393 4_3:+15538485 None:intergenic
TCCACTATCTCTGCTTCAAG+AGG 0.522381 4_3:+15538359 None:intergenic
AATATGTCTAGCTGTGGTTG+TGG 0.535056 4_3:-15539292 Msa0631990:CDS
CCGGCCAAGATCCAATTTGA+AGG 0.537483 4_3:-15538485 Msa0631990:CDS
TGGCATATATCGACATAAGT+TGG 0.537615 4_3:-15538053 Msa0631990:three_prime_UTR
GCCCAAAATCACGGTTTCTG+TGG 0.561475 4_3:+15538514 None:intergenic
GAGGATGGTGGCTGCAAGTG+TGG 0.564753 4_3:-15538440 Msa0631990:CDS
CGGACCAACGCCCAAAATCA+CGG 0.579536 4_3:+15538505 None:intergenic
TGAAATGGGTGTTGCAGCTG+AGG 0.589074 4_3:-15538459 Msa0631990:CDS
ATGGGTGTTGCAGCTGAGGA+TGG 0.598628 4_3:-15538455 Msa0631990:CDS
AGATGCAGCAAGAGGTCTAG+TGG 0.607417 4_3:-15538560 Msa0631990:CDS
TACTTGGCAGATGCAGCAAG+AGG 0.622933 4_3:-15538568 Msa0631990:intron
GGTGTTGCAGCTGAGGATGG+TGG 0.628303 4_3:-15538452 Msa0631990:CDS
TGTGGAAGCAGTTGCAACTG+TGG 0.645986 4_3:-15539274 Msa0631990:CDS
TAGTGGATTGAGCTATGCCG+AGG 0.657350 4_3:-15538543 Msa0631990:CDS
TCTGAAAATATGTCTAGCTG+TGG 0.682059 4_3:-15539298 Msa0631990:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTATATTTCTTTTATGA+GGG - chr4_3:15538489-15538508 Msa0631990:CDS 10.0%
!!! TATAATTGATTTTTATTACT+TGG - chr4_3:15538841-15538860 Msa0631990:intron 10.0%
!!! TATTTATATTTCTTTTATGA+GGG - chr4_3:15538489-15538508 Msa0631990:CDS 10.0%
!!! TATAATTGATTTTTATTACT+TGG - chr4_3:15538841-15538860 Msa0631990:intron 10.0%
!!! AATAGTCATATAATTTTGTT+TGG + chr4_3:15538223-15538242 None:intergenic 15.0%
!!! TTTTAATTTATGATGATTCT+TGG - chr4_3:15538262-15538281 Msa0631990:three_prime_UTR 15.0%
!! GATCATTAAATTAATATACT+TGG - chr4_3:15538418-15538437 Msa0631990:CDS 15.0%
!!! GTATTTATATTTCTTTTATG+AGG - chr4_3:15538488-15538507 Msa0631990:CDS 15.0%
!!! ACTTCATATTATTCTAAAAA+AGG + chr4_3:15539150-15539169 None:intergenic 15.0%
!!! TTTTTTAGAATAATATGAAG+TGG - chr4_3:15539149-15539168 Msa0631990:intron 15.0%
!!! TTGTTTGAATATAAGTTTTT+TGG - chr4_3:15539333-15539352 Msa0631990:five_prime_UTR 15.0%
!!! AATAGTCATATAATTTTGTT+TGG + chr4_3:15538223-15538242 None:intergenic 15.0%
!!! TTTTAATTTATGATGATTCT+TGG - chr4_3:15538262-15538281 Msa0631990:three_prime_UTR 15.0%
!! GATCATTAAATTAATATACT+TGG - chr4_3:15538418-15538437 Msa0631990:CDS 15.0%
!!! GTATTTATATTTCTTTTATG+AGG - chr4_3:15538488-15538507 Msa0631990:CDS 15.0%
!!! ACTTCATATTATTCTAAAAA+AGG + chr4_3:15539150-15539169 None:intergenic 15.0%
!!! TTTTTTAGAATAATATGAAG+TGG - chr4_3:15539149-15539168 Msa0631990:intron 15.0%
!!! TTGTTTGAATATAAGTTTTT+TGG - chr4_3:15539333-15539352 Msa0631990:five_prime_UTR 15.0%
!! AAGAACAAATTTATATTGGT+TGG + chr4_3:15538084-15538103 None:intergenic 20.0%
!! TGCAAAGAACAAATTTATAT+TGG + chr4_3:15538088-15538107 None:intergenic 20.0%
!!! TTTTTTTTCTCTATCTTCAT+AGG - chr4_3:15538341-15538360 Msa0631990:three_prime_UTR 20.0%
!!! AAATTTGATTTGTGTTTAGA+AGG - chr4_3:15538463-15538482 Msa0631990:CDS 20.0%
!!! AATTTGATTTGTGTTTAGAA+GGG - chr4_3:15538464-15538483 Msa0631990:CDS 20.0%
!! AGTGTACAATAATAATTCAT+CGG - chr4_3:15538764-15538783 Msa0631990:intron 20.0%
!!! ATTCAAATGTTCTTAGATTT+TGG - chr4_3:15539194-15539213 Msa0631990:intron 20.0%
!! AAGAACAAATTTATATTGGT+TGG + chr4_3:15538084-15538103 None:intergenic 20.0%
!! TGCAAAGAACAAATTTATAT+TGG + chr4_3:15538088-15538107 None:intergenic 20.0%
!!! TTTTTTTTCTCTATCTTCAT+AGG - chr4_3:15538341-15538360 Msa0631990:three_prime_UTR 20.0%
!!! AAATTTGATTTGTGTTTAGA+AGG - chr4_3:15538463-15538482 Msa0631990:CDS 20.0%
!!! AATTTGATTTGTGTTTAGAA+GGG - chr4_3:15538464-15538483 Msa0631990:CDS 20.0%
!! AGTGTACAATAATAATTCAT+CGG - chr4_3:15538764-15538783 Msa0631990:intron 20.0%
!!! ATTCAAATGTTCTTAGATTT+TGG - chr4_3:15539194-15539213 Msa0631990:intron 20.0%
!!! TGTTTTTTCCATGATTCTTT+TGG - chr4_3:15538295-15538314 Msa0631990:three_prime_UTR 25.0%
!!! ATTTGATTTGTGTTTAGAAG+GGG - chr4_3:15538465-15538484 Msa0631990:CDS 25.0%
!! TAATTCATCGGTATCAATTT+TGG - chr4_3:15538776-15538795 Msa0631990:intron 25.0%
! ATACACACAATTACACATAT+TGG + chr4_3:15539093-15539112 None:intergenic 25.0%
! AGAACATTTGAATTTGATGA+AGG + chr4_3:15539187-15539206 None:intergenic 25.0%
!!! TGTTTTTTCCATGATTCTTT+TGG - chr4_3:15538295-15538314 Msa0631990:three_prime_UTR 25.0%
!!! ATTTGATTTGTGTTTAGAAG+GGG - chr4_3:15538465-15538484 Msa0631990:CDS 25.0%
!! TAATTCATCGGTATCAATTT+TGG - chr4_3:15538776-15538795 Msa0631990:intron 25.0%
! ATACACACAATTACACATAT+TGG + chr4_3:15539093-15539112 None:intergenic 25.0%
! AGAACATTTGAATTTGATGA+AGG + chr4_3:15539187-15539206 None:intergenic 25.0%
AAAGCAACAAAAAGTTAAGG+TGG + chr4_3:15538200-15538219 None:intergenic 30.0%
TGGAAAGCAACAAAAAGTTA+AGG + chr4_3:15538203-15538222 None:intergenic 30.0%
! TTTTCCATGATTCTTTTGGT+TGG - chr4_3:15538299-15538318 Msa0631990:three_prime_UTR 30.0%
!! TTTCCATGATTCTTTTGGTT+GGG - chr4_3:15538300-15538319 Msa0631990:three_prime_UTR 30.0%
!! TTTGATTTGTGTTTAGAAGG+GGG - chr4_3:15538466-15538485 Msa0631990:CDS 30.0%
GTCAAATGCAATTAAGTAAG+AGG - chr4_3:15538599-15538618 Msa0631990:intron 30.0%
!! AACTCTTTACCATTGACTTA+AGG + chr4_3:15538708-15538727 None:intergenic 30.0%
AGAATAATATGAAGTGGTCA+TGG - chr4_3:15539155-15539174 Msa0631990:intron 30.0%
!! ATGTTCTTAGATTTTGGTGA+AGG - chr4_3:15539200-15539219 Msa0631990:intron 30.0%
TTGGTTCATCAATATCAAGT+TGG - chr4_3:15539352-15539371 Msa0631990:five_prime_UTR 30.0%
AAAGCAACAAAAAGTTAAGG+TGG + chr4_3:15538200-15538219 None:intergenic 30.0%
TGGAAAGCAACAAAAAGTTA+AGG + chr4_3:15538203-15538222 None:intergenic 30.0%
! TTTTCCATGATTCTTTTGGT+TGG - chr4_3:15538299-15538318 Msa0631990:three_prime_UTR 30.0%
!! TTTCCATGATTCTTTTGGTT+GGG - chr4_3:15538300-15538319 Msa0631990:three_prime_UTR 30.0%
!! TTTGATTTGTGTTTAGAAGG+GGG - chr4_3:15538466-15538485 Msa0631990:CDS 30.0%
GTCAAATGCAATTAAGTAAG+AGG - chr4_3:15538599-15538618 Msa0631990:intron 30.0%
!! AACTCTTTACCATTGACTTA+AGG + chr4_3:15538708-15538727 None:intergenic 30.0%
AGAATAATATGAAGTGGTCA+TGG - chr4_3:15539155-15539174 Msa0631990:intron 30.0%
!! ATGTTCTTAGATTTTGGTGA+AGG - chr4_3:15539200-15539219 Msa0631990:intron 30.0%
TTGGTTCATCAATATCAAGT+TGG - chr4_3:15539352-15539371 Msa0631990:five_prime_UTR 30.0%
TCTGAAAATATGTCTAGCTG+TGG - chr4_3:15538127-15538146 Msa0631990:three_prime_UTR 35.0%
ATAAGATATGCACAACGACT+TGG - chr4_3:15538674-15538693 Msa0631990:intron 35.0%
!! CAATTTGAAGGTGCTGAAAT+GGG - chr4_3:15538952-15538971 Msa0631990:intron 35.0%
TGGCATATATCGACATAAGT+TGG - chr4_3:15539372-15539391 Msa0631990:five_prime_UTR 35.0%
TCTGAAAATATGTCTAGCTG+TGG - chr4_3:15538127-15538146 Msa0631990:three_prime_UTR 35.0%
ATAAGATATGCACAACGACT+TGG - chr4_3:15538674-15538693 Msa0631990:intron 35.0%
!! CAATTTGAAGGTGCTGAAAT+GGG - chr4_3:15538952-15538971 Msa0631990:intron 35.0%
TGGCATATATCGACATAAGT+TGG - chr4_3:15539372-15539391 Msa0631990:five_prime_UTR 35.0%
AATATGTCTAGCTGTGGTTG+TGG - chr4_3:15538133-15538152 Msa0631990:three_prime_UTR 40.0%
GAACCCAACCAAAAGAATCA+TGG + chr4_3:15538306-15538325 None:intergenic 40.0%
AATCACGGTTTCTGTGGTTT+CGG + chr4_3:15538908-15538927 None:intergenic 40.0%
! AACCACAGAAACCGTGATTT+TGG - chr4_3:15538909-15538928 Msa0631990:intron 40.0%
! ACCACAGAAACCGTGATTTT+GGG - chr4_3:15538910-15538929 Msa0631990:intron 40.0%
AGCACCTTCAAATTGGATCT+TGG + chr4_3:15538947-15538966 None:intergenic 40.0%
CCATTTCAGCACCTTCAAAT+TGG + chr4_3:15538954-15538973 None:intergenic 40.0%
!! CCAATTTGAAGGTGCTGAAA+TGG - chr4_3:15538951-15538970 Msa0631990:intron 40.0%
CACTTCATTTGCAGTTGCAA+GGG + chr4_3:15539029-15539048 None:intergenic 40.0%
AATATGTCTAGCTGTGGTTG+TGG - chr4_3:15538133-15538152 Msa0631990:three_prime_UTR 40.0%
GAACCCAACCAAAAGAATCA+TGG + chr4_3:15538306-15538325 None:intergenic 40.0%
AATCACGGTTTCTGTGGTTT+CGG + chr4_3:15538908-15538927 None:intergenic 40.0%
! AACCACAGAAACCGTGATTT+TGG - chr4_3:15538909-15538928 Msa0631990:intron 40.0%
! ACCACAGAAACCGTGATTTT+GGG - chr4_3:15538910-15538929 Msa0631990:intron 40.0%
AGCACCTTCAAATTGGATCT+TGG + chr4_3:15538947-15538966 None:intergenic 40.0%
CCATTTCAGCACCTTCAAAT+TGG + chr4_3:15538954-15538973 None:intergenic 40.0%
!! CCAATTTGAAGGTGCTGAAA+TGG - chr4_3:15538951-15538970 Msa0631990:intron 40.0%
CACTTCATTTGCAGTTGCAA+GGG + chr4_3:15539029-15539048 None:intergenic 40.0%
TGTGGTGATAGCTGCAAGTA+AGG - chr4_3:15538169-15538188 Msa0631990:three_prime_UTR 45.0%
! AGATATGCACAACGACTTGG+CGG - chr4_3:15538677-15538696 Msa0631990:intron 45.0%
CCTTCAAATTGGATCTTGGC+CGG + chr4_3:15538943-15538962 None:intergenic 45.0%
GCACTTCATTTGCAGTTGCA+AGG + chr4_3:15539030-15539049 None:intergenic 45.0%
TCCACTATCTCTGCTTCAAG+AGG + chr4_3:15539069-15539088 None:intergenic 45.0%
AGCTATGATCGCTTCGTGTT+TGG - chr4_3:15539232-15539251 Msa0631990:intron 45.0%
TGTGGTGATAGCTGCAAGTA+AGG - chr4_3:15538169-15538188 Msa0631990:three_prime_UTR 45.0%
! AGATATGCACAACGACTTGG+CGG - chr4_3:15538677-15538696 Msa0631990:intron 45.0%
CCTTCAAATTGGATCTTGGC+CGG + chr4_3:15538943-15538962 None:intergenic 45.0%
GCACTTCATTTGCAGTTGCA+AGG + chr4_3:15539030-15539049 None:intergenic 45.0%
TCCACTATCTCTGCTTCAAG+AGG + chr4_3:15539069-15539088 None:intergenic 45.0%
AGCTATGATCGCTTCGTGTT+TGG - chr4_3:15539232-15539251 Msa0631990:intron 45.0%
!! TAATATATTCTATTTATAAT+AGG + chr4_3:15539308-15539327 None:intergenic 5.0%
!! TAATATATTCTATTTATAAT+AGG + chr4_3:15539308-15539327 None:intergenic 5.0%
TGTGGAAGCAGTTGCAACTG+TGG - chr4_3:15538151-15538170 Msa0631990:three_prime_UTR 50.0%
! GATATGCACAACGACTTGGC+GGG - chr4_3:15538678-15538697 Msa0631990:intron 50.0%
GCGGGTACTCCTTAAGTCAA+TGG - chr4_3:15538696-15538715 Msa0631990:intron 50.0%
TACTTGGCAGATGCAGCAAG+AGG - chr4_3:15538857-15538876 Msa0631990:intron 50.0%
AGATGCAGCAAGAGGTCTAG+TGG - chr4_3:15538865-15538884 Msa0631990:intron 50.0%
!! TAGTGGATTGAGCTATGCCG+AGG - chr4_3:15538882-15538901 Msa0631990:intron 50.0%
GCCCAAAATCACGGTTTCTG+TGG + chr4_3:15538914-15538933 None:intergenic 50.0%
! GAAACCGTGATTTTGGGCGT+TGG - chr4_3:15538916-15538935 Msa0631990:intron 50.0%
CCGGCCAAGATCCAATTTGA+AGG - chr4_3:15538940-15538959 Msa0631990:intron 50.0%
! TGAAATGGGTGTTGCAGCTG+AGG - chr4_3:15538966-15538985 Msa0631990:intron 50.0%
GCCTCTTGAAGCAGAGATAG+TGG - chr4_3:15539065-15539084 Msa0631990:intron 50.0%
TGTGGAAGCAGTTGCAACTG+TGG - chr4_3:15538151-15538170 Msa0631990:three_prime_UTR 50.0%
! GATATGCACAACGACTTGGC+GGG - chr4_3:15538678-15538697 Msa0631990:intron 50.0%
GCGGGTACTCCTTAAGTCAA+TGG - chr4_3:15538696-15538715 Msa0631990:intron 50.0%
TACTTGGCAGATGCAGCAAG+AGG - chr4_3:15538857-15538876 Msa0631990:intron 50.0%
AGATGCAGCAAGAGGTCTAG+TGG - chr4_3:15538865-15538884 Msa0631990:intron 50.0%
!! TAGTGGATTGAGCTATGCCG+AGG - chr4_3:15538882-15538901 Msa0631990:intron 50.0%
GCCCAAAATCACGGTTTCTG+TGG + chr4_3:15538914-15538933 None:intergenic 50.0%
! GAAACCGTGATTTTGGGCGT+TGG - chr4_3:15538916-15538935 Msa0631990:intron 50.0%
CCGGCCAAGATCCAATTTGA+AGG - chr4_3:15538940-15538959 Msa0631990:intron 50.0%
! TGAAATGGGTGTTGCAGCTG+AGG - chr4_3:15538966-15538985 Msa0631990:intron 50.0%
GCCTCTTGAAGCAGAGATAG+TGG - chr4_3:15539065-15539084 Msa0631990:intron 50.0%
GGTTTCTGTGGTTTCGGCCT+CGG + chr4_3:15538902-15538921 None:intergenic 55.0%
CGGACCAACGCCCAAAATCA+CGG + chr4_3:15538923-15538942 None:intergenic 55.0%
!!! CGTGATTTTGGGCGTTGGTC+CGG - chr4_3:15538921-15538940 Msa0631990:intron 55.0%
! ATGGGTGTTGCAGCTGAGGA+TGG - chr4_3:15538970-15538989 Msa0631990:intron 55.0%
GGTTTCTGTGGTTTCGGCCT+CGG + chr4_3:15538902-15538921 None:intergenic 55.0%
CGGACCAACGCCCAAAATCA+CGG + chr4_3:15538923-15538942 None:intergenic 55.0%
!!! CGTGATTTTGGGCGTTGGTC+CGG - chr4_3:15538921-15538940 Msa0631990:intron 55.0%
! ATGGGTGTTGCAGCTGAGGA+TGG - chr4_3:15538970-15538989 Msa0631990:intron 55.0%
! GGTGTTGCAGCTGAGGATGG+TGG - chr4_3:15538973-15538992 Msa0631990:intron 60.0%
! GAGGATGGTGGCTGCAAGTG+TGG - chr4_3:15538985-15539004 Msa0631990:intron 60.0%
! GGTGTTGCAGCTGAGGATGG+TGG - chr4_3:15538973-15538992 Msa0631990:intron 60.0%
! GAGGATGGTGGCTGCAAGTG+TGG - chr4_3:15538985-15539004 Msa0631990:intron 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 15538051 15539396 15538051 ID=Msa0631990;Name=Msa0631990
chr4_3 mRNA 15538051 15539396 15538051 ID=Msa0631990-mRNA-1;Parent=Msa0631990;Name=Msa0631990-mRNA-1;_AED=0.00;_eAED=0.00;_QI=85|1|1|1|1|1|2|352|75
chr4_3 exon 15538051 15538580 15538051 ID=Msa0631990-mRNA-1:exon:4416;Parent=Msa0631990-mRNA-1
chr4_3 exon 15539262 15539396 15539262 ID=Msa0631990-mRNA-1:exon:4415;Parent=Msa0631990-mRNA-1
chr4_3 five_prime_UTR 15539312 15539396 15539312 ID=Msa0631990-mRNA-1:five_prime_utr;Parent=Msa0631990-mRNA-1
chr4_3 CDS 15539262 15539311 15539262 ID=Msa0631990-mRNA-1:cds;Parent=Msa0631990-mRNA-1
chr4_3 CDS 15538403 15538580 15538403 ID=Msa0631990-mRNA-1:cds;Parent=Msa0631990-mRNA-1
chr4_3 three_prime_UTR 15538051 15538402 15538051 ID=Msa0631990-mRNA-1:three_prime_utr;Parent=Msa0631990-mRNA-1
Gene Sequence

>Msa0631990

ATGTCTAGCTGTGGTTGTGGAAGCAGTTGCAACTGTGGTGATAGCTGCAAATGCAGCAAGAGGTCTAGTGGATTGAGCTATGCCGAGGCCGAAACCACAGAAACCGTGATTTTGGGCGTTGGTCCGGCCAAGATCCAATTTGAAGGTGCTGAAATGGGTGTTGCAGCTGAGGATGGTGGCTGCAAGTGTGGAGATAACTGCACATGTGACCCTTGCAACTGCAAATGA

Protein sequence

>Msa0631990

MSSCGCGSSCNCGDSCKCSKRSSGLSYAEAETTETVILGVGPAKIQFEGAEMGVAAEDGGCKCGDNCTCDPCNCK*