AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1279650


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1279650 MtrunA17_Chr8g0360121 98.413 63 1 0 1 63 1 63 3.06e-40 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1279650 AT3G15353.1 57.576 66 23 2 1 61 1 66 3.08e-17 68.6
Msa1279650 AT3G15353.2 56.923 65 23 2 1 61 1 64 1.66e-16 66.6

Find 21 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATGTTGTGGCATTGTTAC+TGG 0.182186 8_3:-16897147 Msa1279650:three_prime_UTR
AAATGTAATTGCCGTGAATT+TGG 0.266307 8_3:-16897268 Msa1279650:three_prime_UTR
CATGTTGTGGCATTGTTACT+GGG 0.421135 8_3:-16897146 Msa1279650:three_prime_UTR
ATGAGCTTGACATGTTTGTT+TGG 0.442431 8_3:+16898012 None:intergenic
TTGCAATCATGCTCAACAGC+TGG 0.507300 8_3:+16897348 None:intergenic
ATATATTCTTTAATGACCGC+AGG 0.551610 8_3:+16897293 None:intergenic
ATTATACAACTACCATGTTG+TGG 0.572220 8_3:-16897159 Msa1279650:three_prime_UTR
AGAGGAGCACTTACCCGCAC+TGG 0.574771 8_3:+16897961 None:intergenic
GTTGAGCATGATTGCAAGTG+TGG 0.581040 8_3:-16897342 Msa1279650:CDS
CTTGGTCGAGACTGTTGTCA+TGG 0.585178 8_3:-16897376 Msa1279650:CDS
TTCAGGAAGGGAAACAACTA+CGG 0.588697 8_3:-16897540 Msa1279650:intron
GCGCTGACAAGAGCCAGTGC+GGG 0.597634 8_3:-16897974 Msa1279650:intron
ACAAACATGTCAAGCTCATG+CGG 0.602184 8_3:-16898008 Msa1279650:exon
TGCGCTGACAAGAGCCAGTG+CGG 0.611618 8_3:-16897975 Msa1279650:CDS
CCAGTAACAATGCCACAACA+TGG 0.639380 8_3:+16897147 None:intergenic
ACTTGCACTAACTGCACCTG+CGG 0.677689 8_3:-16897309 Msa1279650:CDS
CCTCTTCTGAGTCTCGACAA+TGG 0.679042 8_3:+16897512 None:intergenic
AAGATATGTGCTGATGTCCA+TGG 0.681433 8_3:+16897072 None:intergenic
CCATTGTCGAGACTCAGAAG+AGG 0.690774 8_3:-16897512 Msa1279650:intron
TAATGCTGCTGAGACCACCA+TGG 0.722784 8_3:-16897089 Msa1279650:three_prime_UTR
ATATGTGCTGATGTCCATGG+TGG 0.840627 8_3:+16897075 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAACACTTATGATATAAG+TGG - chr8_3:16897262-16897281 Msa1279650:three_prime_UTR 20.0%
!! GAGTTTATAAAAATAGCTTA+TGG + chr8_3:16897383-16897402 None:intergenic 20.0%
!! ATAAACACTTATGATATAAG+TGG - chr8_3:16897262-16897281 Msa1279650:three_prime_UTR 20.0%
!! GAGTTTATAAAAATAGCTTA+TGG + chr8_3:16897383-16897402 None:intergenic 20.0%
! TTTAGAAAAACGGCTTAATT+AGG - chr8_3:16897229-16897248 Msa1279650:three_prime_UTR 25.0%
! ATTATACACAATTTAAGCTC+AGG - chr8_3:16898064-16898083 Msa1279650:five_prime_UTR 25.0%
! TTTAGAAAAACGGCTTAATT+AGG - chr8_3:16897229-16897248 Msa1279650:three_prime_UTR 25.0%
AAGGTTAATGTTGTACTGTA+AGG - chr8_3:16897130-16897149 Msa1279650:three_prime_UTR 30.0%
!!! TTAAGCCGTTTTTCTAAACA+AGG + chr8_3:16897227-16897246 None:intergenic 30.0%
! TTGTTTTCATAAGCTGAAGT+CGG - chr8_3:16897345-16897364 Msa1279650:CDS 30.0%
TACCATAGCATCATATATGA+GGG + chr8_3:16897496-16897515 None:intergenic 30.0%
ATACCATAGCATCATATATG+AGG + chr8_3:16897497-16897516 None:intergenic 30.0%
TATATGATGCTATGGTATTC+AGG - chr8_3:16897499-16897518 Msa1279650:intron 30.0%
ATATATTCTTTAATGACCGC+AGG + chr8_3:16897766-16897785 None:intergenic 30.0%
AAATGTAATTGCCGTGAATT+TGG - chr8_3:16897788-16897807 Msa1279650:intron 30.0%
! GTTTTGTATTGCCAAATTCA+CGG + chr8_3:16897802-16897821 None:intergenic 30.0%
ATTATACAACTACCATGTTG+TGG - chr8_3:16897897-16897916 Msa1279650:intron 30.0%
AAGGTTAATGTTGTACTGTA+AGG - chr8_3:16897130-16897149 Msa1279650:three_prime_UTR 30.0%
!!! TTAAGCCGTTTTTCTAAACA+AGG + chr8_3:16897227-16897246 None:intergenic 30.0%
! TTGTTTTCATAAGCTGAAGT+CGG - chr8_3:16897345-16897364 Msa1279650:CDS 30.0%
TACCATAGCATCATATATGA+GGG + chr8_3:16897496-16897515 None:intergenic 30.0%
ATACCATAGCATCATATATG+AGG + chr8_3:16897497-16897516 None:intergenic 30.0%
TATATGATGCTATGGTATTC+AGG - chr8_3:16897499-16897518 Msa1279650:intron 30.0%
ATATATTCTTTAATGACCGC+AGG + chr8_3:16897766-16897785 None:intergenic 30.0%
AAATGTAATTGCCGTGAATT+TGG - chr8_3:16897788-16897807 Msa1279650:intron 30.0%
! GTTTTGTATTGCCAAATTCA+CGG + chr8_3:16897802-16897821 None:intergenic 30.0%
ATTATACAACTACCATGTTG+TGG - chr8_3:16897897-16897916 Msa1279650:intron 30.0%
ATGAGCTTGACATGTTTGTT+TGG + chr8_3:16897047-16897066 None:intergenic 35.0%
AACCTTACAGTACAAGATAG+AGG + chr8_3:16897116-16897135 None:intergenic 35.0%
GCGTTCACTCATTTATATAG+AGG - chr8_3:16897201-16897220 Msa1279650:three_prime_UTR 35.0%
!! AGAGGCCTTGTTTAGAAAAA+CGG - chr8_3:16897219-16897238 Msa1279650:three_prime_UTR 35.0%
CAGCTTATGAAAACAACTTC+AGG + chr8_3:16897341-16897360 None:intergenic 35.0%
ACCATAGCATCATATATGAG+GGG + chr8_3:16897495-16897514 None:intergenic 35.0%
TCGAGGAGTTATTAGAAACA+TGG + chr8_3:16897599-16897618 None:intergenic 35.0%
ATGAGCTTGACATGTTTGTT+TGG + chr8_3:16897047-16897066 None:intergenic 35.0%
AACCTTACAGTACAAGATAG+AGG + chr8_3:16897116-16897135 None:intergenic 35.0%
GCGTTCACTCATTTATATAG+AGG - chr8_3:16897201-16897220 Msa1279650:three_prime_UTR 35.0%
!! AGAGGCCTTGTTTAGAAAAA+CGG - chr8_3:16897219-16897238 Msa1279650:three_prime_UTR 35.0%
CAGCTTATGAAAACAACTTC+AGG + chr8_3:16897341-16897360 None:intergenic 35.0%
ACCATAGCATCATATATGAG+GGG + chr8_3:16897495-16897514 None:intergenic 35.0%
TCGAGGAGTTATTAGAAACA+TGG + chr8_3:16897599-16897618 None:intergenic 35.0%
ACAAACATGTCAAGCTCATG+CGG - chr8_3:16897048-16897067 Msa1279650:three_prime_UTR 40.0%
CTCCTCTATCTTGTACTGTA+AGG - chr8_3:16897111-16897130 Msa1279650:three_prime_UTR 40.0%
! TACGGATTTGTTTGTGAGAC+TGG + chr8_3:16897412-16897431 None:intergenic 40.0%
TCTCACAAACAAATCCGTAC+AGG - chr8_3:16897413-16897432 Msa1279650:intron 40.0%
TCCCCTCATATATGATGCTA+TGG - chr8_3:16897491-16897510 Msa1279650:intron 40.0%
TGATGCTATGGTATTCAGGA+AGG - chr8_3:16897503-16897522 Msa1279650:intron 40.0%
GATGCTATGGTATTCAGGAA+GGG - chr8_3:16897504-16897523 Msa1279650:intron 40.0%
TTCAGGAAGGGAAACAACTA+CGG - chr8_3:16897516-16897535 Msa1279650:CDS 40.0%
! GTCAAATGTTTTGTGCAGCT+TGG - chr8_3:16897662-16897681 Msa1279650:intron 40.0%
CATGTTGTGGCATTGTTACT+GGG - chr8_3:16897910-16897929 Msa1279650:intron 40.0%
! AAGATATGTGCTGATGTCCA+TGG + chr8_3:16897987-16898006 None:intergenic 40.0%
ACAAACATGTCAAGCTCATG+CGG - chr8_3:16897048-16897067 Msa1279650:three_prime_UTR 40.0%
CTCCTCTATCTTGTACTGTA+AGG - chr8_3:16897111-16897130 Msa1279650:three_prime_UTR 40.0%
! TACGGATTTGTTTGTGAGAC+TGG + chr8_3:16897412-16897431 None:intergenic 40.0%
TCTCACAAACAAATCCGTAC+AGG - chr8_3:16897413-16897432 Msa1279650:intron 40.0%
TCCCCTCATATATGATGCTA+TGG - chr8_3:16897491-16897510 Msa1279650:intron 40.0%
TGATGCTATGGTATTCAGGA+AGG - chr8_3:16897503-16897522 Msa1279650:intron 40.0%
GATGCTATGGTATTCAGGAA+GGG - chr8_3:16897504-16897523 Msa1279650:intron 40.0%
TTCAGGAAGGGAAACAACTA+CGG - chr8_3:16897516-16897535 Msa1279650:CDS 40.0%
! GTCAAATGTTTTGTGCAGCT+TGG - chr8_3:16897662-16897681 Msa1279650:intron 40.0%
CATGTTGTGGCATTGTTACT+GGG - chr8_3:16897910-16897929 Msa1279650:intron 40.0%
! AAGATATGTGCTGATGTCCA+TGG + chr8_3:16897987-16898006 None:intergenic 40.0%
! CATAAGCTGAAGTCGGCTTA+TGG - chr8_3:16897352-16897371 Msa1279650:CDS 45.0%
AACAAATCCGTACAGGCTTG+TGG - chr8_3:16897420-16897439 Msa1279650:intron 45.0%
!! CTAGCTTTTGCATTGAGTCG+AGG + chr8_3:16897616-16897635 None:intergenic 45.0%
TTGCAATCATGCTCAACAGC+TGG + chr8_3:16897711-16897730 None:intergenic 45.0%
GTTGAGCATGATTGCAAGTG+TGG - chr8_3:16897714-16897733 Msa1279650:intron 45.0%
CCAGTAACAATGCCACAACA+TGG + chr8_3:16897912-16897931 None:intergenic 45.0%
CCATGTTGTGGCATTGTTAC+TGG - chr8_3:16897909-16897928 Msa1279650:intron 45.0%
! ATATGTGCTGATGTCCATGG+TGG + chr8_3:16897984-16898003 None:intergenic 45.0%
! CATAAGCTGAAGTCGGCTTA+TGG - chr8_3:16897352-16897371 Msa1279650:CDS 45.0%
AACAAATCCGTACAGGCTTG+TGG - chr8_3:16897420-16897439 Msa1279650:intron 45.0%
!! CTAGCTTTTGCATTGAGTCG+AGG + chr8_3:16897616-16897635 None:intergenic 45.0%
TTGCAATCATGCTCAACAGC+TGG + chr8_3:16897711-16897730 None:intergenic 45.0%
GTTGAGCATGATTGCAAGTG+TGG - chr8_3:16897714-16897733 Msa1279650:intron 45.0%
CCAGTAACAATGCCACAACA+TGG + chr8_3:16897912-16897931 None:intergenic 45.0%
CCATGTTGTGGCATTGTTAC+TGG - chr8_3:16897909-16897928 Msa1279650:intron 45.0%
! ATATGTGCTGATGTCCATGG+TGG + chr8_3:16897984-16898003 None:intergenic 45.0%
!!! TATTTCTATAAATAAAATAA+AGG - chr8_3:16897297-16897316 Msa1279650:exon 5.0%
!!! TATTTCTATAAATAAAATAA+AGG - chr8_3:16897297-16897316 Msa1279650:exon 5.0%
CTACAAGCCACAAGCCTGTA+CGG + chr8_3:16897430-16897449 None:intergenic 50.0%
CCTCTTCTGAGTCTCGACAA+TGG + chr8_3:16897547-16897566 None:intergenic 50.0%
CCATTGTCGAGACTCAGAAG+AGG - chr8_3:16897544-16897563 Msa1279650:intron 50.0%
CTTGGTCGAGACTGTTGTCA+TGG - chr8_3:16897680-16897699 Msa1279650:intron 50.0%
ACTTGCACTAACTGCACCTG+CGG - chr8_3:16897747-16897766 Msa1279650:intron 50.0%
TAATGCTGCTGAGACCACCA+TGG - chr8_3:16897967-16897986 Msa1279650:intron 50.0%
CTACAAGCCACAAGCCTGTA+CGG + chr8_3:16897430-16897449 None:intergenic 50.0%
CCTCTTCTGAGTCTCGACAA+TGG + chr8_3:16897547-16897566 None:intergenic 50.0%
CCATTGTCGAGACTCAGAAG+AGG - chr8_3:16897544-16897563 Msa1279650:intron 50.0%
CTTGGTCGAGACTGTTGTCA+TGG - chr8_3:16897680-16897699 Msa1279650:intron 50.0%
ACTTGCACTAACTGCACCTG+CGG - chr8_3:16897747-16897766 Msa1279650:intron 50.0%
TAATGCTGCTGAGACCACCA+TGG - chr8_3:16897967-16897986 Msa1279650:intron 50.0%
! TGCGCTGACAAGAGCCAGTG+CGG - chr8_3:16897081-16897100 Msa1279650:three_prime_UTR 60.0%
!! AGAGGAGCACTTACCCGCAC+TGG + chr8_3:16897098-16897117 None:intergenic 60.0%
! TGCGCTGACAAGAGCCAGTG+CGG - chr8_3:16897081-16897100 Msa1279650:three_prime_UTR 60.0%
!! AGAGGAGCACTTACCCGCAC+TGG + chr8_3:16897098-16897117 None:intergenic 60.0%
! GCGCTGACAAGAGCCAGTGC+GGG - chr8_3:16897082-16897101 Msa1279650:three_prime_UTR 65.0%
! GCGCTGACAAGAGCCAGTGC+GGG - chr8_3:16897082-16897101 Msa1279650:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 16896993 16898085 16896993 ID=Msa1279650;Name=Msa1279650
chr8_3 mRNA 16896993 16898085 16896993 ID=Msa1279650-mRNA-1;Parent=Msa1279650;Name=Msa1279650-mRNA-1;_AED=0.08;_eAED=0.08;_QI=61|1|1|1|1|1|3|309|63
chr8_3 exon 16896993 16897398 16896993 ID=Msa1279650-mRNA-1:exon:6596;Parent=Msa1279650-mRNA-1
chr8_3 exon 16897513 16897557 16897513 ID=Msa1279650-mRNA-1:exon:6595;Parent=Msa1279650-mRNA-1
chr8_3 exon 16897975 16898085 16897975 ID=Msa1279650-mRNA-1:exon:6594;Parent=Msa1279650-mRNA-1
chr8_3 five_prime_UTR 16898025 16898085 16898025 ID=Msa1279650-mRNA-1:five_prime_utr;Parent=Msa1279650-mRNA-1
chr8_3 CDS 16897975 16898024 16897975 ID=Msa1279650-mRNA-1:cds;Parent=Msa1279650-mRNA-1
chr8_3 CDS 16897513 16897557 16897513 ID=Msa1279650-mRNA-1:cds;Parent=Msa1279650-mRNA-1
chr8_3 CDS 16897302 16897398 16897302 ID=Msa1279650-mRNA-1:cds;Parent=Msa1279650-mRNA-1
chr8_3 three_prime_UTR 16896993 16897301 16896993 ID=Msa1279650-mRNA-1:three_prime_utr;Parent=Msa1279650-mRNA-1
Gene Sequence

>Msa1279650

ATGTCAAGCTCATGCGGCAACTGCGACTGCGCTGACAAGAGCCAGTGCGGGAAGGGAAACAACTACGGCATGACCATTGTCGAGACTCAGAAGAGCTTGGTCGAGACTGTTGTCATGGATGCTCCAGCTGTTGAGCATGATTGCAAGTGTGGTTCTAACTGCACTTGCACTAACTGCACCTGCGGTCATTAA

Protein sequence

>Msa1279650

MSSSCGNCDCADKSQCGKGNNYGMTIVETQKSLVETVVMDAPAVEHDCKCGSNCTCTNCTCGH*