AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0670660


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0670660 MtrunA17_Chr4g0009821 94.667 75 4 0 1 75 1 75 1.31e-45 140
Msa0670660 MtrunA17_Chr4g0009811 82.667 75 13 0 1 75 1 75 1.70e-39 124
Msa0670660 MtrunA17_Chr7g0238571 56.522 69 27 2 8 75 12 78 1.71e-13 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 35 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCAAATGTTCTTAGATTT+TGG 0.126318 4_4:+13254517 Msa0670660:three_prime_UTR
AACCACAGAAACCGTGATTT+TGG 0.184588 4_4:+13254232 Msa0670660:CDS
CCATTTCAGCACCTTCAAAT+TGG 0.222108 4_4:-13254274 None:intergenic
TAAATCTCTGCGTTTATAAA+TGG 0.225060 4_4:+13254619 Msa0670660:three_prime_UTR
TGCAAAGAACAAATTTATAT+TGG 0.266509 4_4:-13253416 None:intergenic
GGTTTCTGTGGTTTCGGCCT+CGG 0.316189 4_4:-13254222 None:intergenic
AATCACGGTTTCTGTGGTTT+CGG 0.340586 4_4:-13254228 None:intergenic
ATACACACAATTACACATAT+TGG 0.348552 4_4:-13254413 None:intergenic
AGCACCTTCAAATTGGATCT+TGG 0.377145 4_4:-13254267 None:intergenic
GCACTTCATTTGCAGTTGCA+AGG 0.406733 4_4:-13254350 None:intergenic
CCAATTTGAAGGTGCTGAAA+TGG 0.415709 4_4:+13254274 Msa0670660:CDS
AGCTATGATCGCTTTGTGTT+TGG 0.422847 4_4:+13254555 Msa0670660:three_prime_UTR
GCCTCTTGAAGCAGAGATAG+TGG 0.443076 4_4:+13254388 Msa0670660:three_prime_UTR
AAGAACAAATTTATATTGGT+TGG 0.448696 4_4:-13253412 None:intergenic
AGAACATTTGAATTTGATGA+AGG 0.454645 4_4:-13254507 None:intergenic
CACTTCATTTGCAGTTGCAA+GGG 0.477731 4_4:-13254349 None:intergenic
CGGCATTTATCGACATAAGT+TGG 0.494265 4_4:+13254697 Msa0670660:three_prime_UTR
AGAATAATATGAAGTGGTCA+TGG 0.503092 4_4:+13254478 Msa0670660:three_prime_UTR
CAATTTGAAGGTGCTGAAAT+GGG 0.519160 4_4:+13254275 Msa0670660:CDS
CCTTCAAATTGGATCTTGGC+CGG 0.520393 4_4:-13254263 None:intergenic
TCCACTATCTCTGCTTCAAG+AGG 0.522381 4_4:-13254389 None:intergenic
AATATGTCTAGCTGTGGTTG+TGG 0.535056 4_4:+13253464 Msa0670660:exon
CCGGCCAAGATCCAATTTGA+AGG 0.537483 4_4:+13254263 Msa0670660:CDS
TACTTTGCAGATGCAGCAAG+AGG 0.554610 4_4:+13254180 Msa0670660:intron
GCCCAAAATCACGGTTTCTG+TGG 0.561475 4_4:-13254234 None:intergenic
GAGGATGGTGGCTGCAAGTG+TGG 0.564753 4_4:+13254308 Msa0670660:CDS
CGGACCAACGCCCAAAATCA+CGG 0.579536 4_4:-13254243 None:intergenic
TGAAATGGGTGTTGCAGCTG+AGG 0.589074 4_4:+13254289 Msa0670660:CDS
TTGATTCATCAACATCAAGT+CGG 0.591842 4_4:+13254677 Msa0670660:three_prime_UTR
ATGGGTGTTGCAGCTGAGGA+TGG 0.598628 4_4:+13254293 Msa0670660:CDS
AGATGCAGCAAGAGGTCTAG+TGG 0.623429 4_4:+13254188 Msa0670660:intron
GGTGTTGCAGCTGAGGATGG+TGG 0.628303 4_4:+13254296 Msa0670660:CDS
TGTGGAAGCAGTTGCAACTG+TGG 0.645986 4_4:+13253482 Msa0670660:CDS
TAGTGGATTGAGCTATGCCG+AGG 0.657350 4_4:+13254205 Msa0670660:CDS
TCTGAAAATATGTCTAGCTG+TGG 0.682059 4_4:+13253458 Msa0670660:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTATATTTCTTTTATGA+GGG + chr4_4:13253817-13253836 Msa0670660:intron 10.0%
!!! TATTTATATTTCTTTTATGA+GGG + chr4_4:13253817-13253836 Msa0670660:intron 10.0%
!!! AATAGTCATATAATTTTGTT+TGG - chr4_4:13253554-13253573 None:intergenic 15.0%
!!! TTTTAATTTATGATGATTCT+TGG + chr4_4:13253591-13253610 Msa0670660:intron 15.0%
!! GATCATTAAATTAATATACT+TGG + chr4_4:13253746-13253765 Msa0670660:intron 15.0%
!!! GTATTTATATTTCTTTTATG+AGG + chr4_4:13253816-13253835 Msa0670660:intron 15.0%
!!! ACTTCATATTATTCTAAAAA+AGG - chr4_4:13254473-13254492 None:intergenic 15.0%
!!! TTTTTTAGAATAATATGAAG+TGG + chr4_4:13254472-13254491 Msa0670660:three_prime_UTR 15.0%
!!! AATAGTCATATAATTTTGTT+TGG - chr4_4:13253554-13253573 None:intergenic 15.0%
!!! TTTTAATTTATGATGATTCT+TGG + chr4_4:13253591-13253610 Msa0670660:intron 15.0%
!! GATCATTAAATTAATATACT+TGG + chr4_4:13253746-13253765 Msa0670660:intron 15.0%
!!! GTATTTATATTTCTTTTATG+AGG + chr4_4:13253816-13253835 Msa0670660:intron 15.0%
!!! ACTTCATATTATTCTAAAAA+AGG - chr4_4:13254473-13254492 None:intergenic 15.0%
!!! TTTTTTAGAATAATATGAAG+TGG + chr4_4:13254472-13254491 Msa0670660:three_prime_UTR 15.0%
!! AAGAACAAATTTATATTGGT+TGG - chr4_4:13253415-13253434 None:intergenic 20.0%
!! TGCAAAGAACAAATTTATAT+TGG - chr4_4:13253419-13253438 None:intergenic 20.0%
!!! TTTTTTTTCTCTATCTTCAT+AGG + chr4_4:13253670-13253689 Msa0670660:intron 20.0%
!!! AAATTTGATTTGTGTTTAGA+AGG + chr4_4:13253791-13253810 Msa0670660:intron 20.0%
!!! AATTTGATTTGTGTTTAGAA+GGG + chr4_4:13253792-13253811 Msa0670660:intron 20.0%
!! AGTGTACAATAATAATTCAT+CGG + chr4_4:13254087-13254106 Msa0670660:intron 20.0%
!!! ATTCAAATGTTCTTAGATTT+TGG + chr4_4:13254517-13254536 Msa0670660:three_prime_UTR 20.0%
!! AAGAACAAATTTATATTGGT+TGG - chr4_4:13253415-13253434 None:intergenic 20.0%
!! TGCAAAGAACAAATTTATAT+TGG - chr4_4:13253419-13253438 None:intergenic 20.0%
!!! TTTTTTTTCTCTATCTTCAT+AGG + chr4_4:13253670-13253689 Msa0670660:intron 20.0%
!!! AAATTTGATTTGTGTTTAGA+AGG + chr4_4:13253791-13253810 Msa0670660:intron 20.0%
!!! AATTTGATTTGTGTTTAGAA+GGG + chr4_4:13253792-13253811 Msa0670660:intron 20.0%
!! AGTGTACAATAATAATTCAT+CGG + chr4_4:13254087-13254106 Msa0670660:intron 20.0%
!!! ATTCAAATGTTCTTAGATTT+TGG + chr4_4:13254517-13254536 Msa0670660:three_prime_UTR 20.0%
!!! TGTTTTTTCCATGATTCTTT+TGG + chr4_4:13253624-13253643 Msa0670660:intron 25.0%
!!! ATTTGATTTGTGTTTAGAAG+GGG + chr4_4:13253793-13253812 Msa0670660:intron 25.0%
!! TAATTCATCGGTATCAATTT+TGG + chr4_4:13254099-13254118 Msa0670660:intron 25.0%
! ATACACACAATTACACATAT+TGG - chr4_4:13254416-13254435 None:intergenic 25.0%
! AGAACATTTGAATTTGATGA+AGG - chr4_4:13254510-13254529 None:intergenic 25.0%
! TAAATCTCTGCGTTTATAAA+TGG + chr4_4:13254619-13254638 Msa0670660:three_prime_UTR 25.0%
!!! TGTTTTTTCCATGATTCTTT+TGG + chr4_4:13253624-13253643 Msa0670660:intron 25.0%
!!! ATTTGATTTGTGTTTAGAAG+GGG + chr4_4:13253793-13253812 Msa0670660:intron 25.0%
!! TAATTCATCGGTATCAATTT+TGG + chr4_4:13254099-13254118 Msa0670660:intron 25.0%
! ATACACACAATTACACATAT+TGG - chr4_4:13254416-13254435 None:intergenic 25.0%
! AGAACATTTGAATTTGATGA+AGG - chr4_4:13254510-13254529 None:intergenic 25.0%
! TAAATCTCTGCGTTTATAAA+TGG + chr4_4:13254619-13254638 Msa0670660:three_prime_UTR 25.0%
AAAGCAACAAAAAGTTAAGG+TGG - chr4_4:13253531-13253550 None:intergenic 30.0%
TGGAAAGCAACAAAAAGTTA+AGG - chr4_4:13253534-13253553 None:intergenic 30.0%
! TTTTCCATGATTCTTTTGGT+TGG + chr4_4:13253628-13253647 Msa0670660:intron 30.0%
!! TTTCCATGATTCTTTTGGTT+GGG + chr4_4:13253629-13253648 Msa0670660:intron 30.0%
!! TTTGATTTGTGTTTAGAAGG+GGG + chr4_4:13253794-13253813 Msa0670660:intron 30.0%
TGTCAATGCAATTAAGTAAG+AGG + chr4_4:13253926-13253945 Msa0670660:intron 30.0%
AGAATAATATGAAGTGGTCA+TGG + chr4_4:13254478-13254497 Msa0670660:three_prime_UTR 30.0%
!! ATGTTCTTAGATTTTGGTGA+AGG + chr4_4:13254523-13254542 Msa0670660:three_prime_UTR 30.0%
TTGATTCATCAACATCAAGT+CGG + chr4_4:13254677-13254696 Msa0670660:three_prime_UTR 30.0%
AAAGCAACAAAAAGTTAAGG+TGG - chr4_4:13253531-13253550 None:intergenic 30.0%
TGGAAAGCAACAAAAAGTTA+AGG - chr4_4:13253534-13253553 None:intergenic 30.0%
! TTTTCCATGATTCTTTTGGT+TGG + chr4_4:13253628-13253647 Msa0670660:intron 30.0%
!! TTTCCATGATTCTTTTGGTT+GGG + chr4_4:13253629-13253648 Msa0670660:intron 30.0%
!! TTTGATTTGTGTTTAGAAGG+GGG + chr4_4:13253794-13253813 Msa0670660:intron 30.0%
TGTCAATGCAATTAAGTAAG+AGG + chr4_4:13253926-13253945 Msa0670660:intron 30.0%
AGAATAATATGAAGTGGTCA+TGG + chr4_4:13254478-13254497 Msa0670660:three_prime_UTR 30.0%
!! ATGTTCTTAGATTTTGGTGA+AGG + chr4_4:13254523-13254542 Msa0670660:three_prime_UTR 30.0%
TTGATTCATCAACATCAAGT+CGG + chr4_4:13254677-13254696 Msa0670660:three_prime_UTR 30.0%
TCTGAAAATATGTCTAGCTG+TGG + chr4_4:13253458-13253477 Msa0670660:exon 35.0%
ATAAGATATGCACAACGACT+TGG + chr4_4:13254001-13254020 Msa0670660:intron 35.0%
!! AACTCTTTACCATTGACTTG+AGG - chr4_4:13254035-13254054 None:intergenic 35.0%
!! CAATTTGAAGGTGCTGAAAT+GGG + chr4_4:13254275-13254294 Msa0670660:CDS 35.0%
TCTGAAAATATGTCTAGCTG+TGG + chr4_4:13253458-13253477 Msa0670660:exon 35.0%
ATAAGATATGCACAACGACT+TGG + chr4_4:13254001-13254020 Msa0670660:intron 35.0%
!! AACTCTTTACCATTGACTTG+AGG - chr4_4:13254035-13254054 None:intergenic 35.0%
!! CAATTTGAAGGTGCTGAAAT+GGG + chr4_4:13254275-13254294 Msa0670660:CDS 35.0%
AATATGTCTAGCTGTGGTTG+TGG + chr4_4:13253464-13253483 Msa0670660:exon 40.0%
GAACCCAACCAAAAGAATCA+TGG - chr4_4:13253635-13253654 None:intergenic 40.0%
AATCACGGTTTCTGTGGTTT+CGG - chr4_4:13254231-13254250 None:intergenic 40.0%
! AACCACAGAAACCGTGATTT+TGG + chr4_4:13254232-13254251 Msa0670660:CDS 40.0%
! ACCACAGAAACCGTGATTTT+GGG + chr4_4:13254233-13254252 Msa0670660:CDS 40.0%
AGCACCTTCAAATTGGATCT+TGG - chr4_4:13254270-13254289 None:intergenic 40.0%
CCATTTCAGCACCTTCAAAT+TGG - chr4_4:13254277-13254296 None:intergenic 40.0%
!! CCAATTTGAAGGTGCTGAAA+TGG + chr4_4:13254274-13254293 Msa0670660:CDS 40.0%
CACTTCATTTGCAGTTGCAA+GGG - chr4_4:13254352-13254371 None:intergenic 40.0%
AGCTATGATCGCTTTGTGTT+TGG + chr4_4:13254555-13254574 Msa0670660:three_prime_UTR 40.0%
CGGCATTTATCGACATAAGT+TGG + chr4_4:13254697-13254716 Msa0670660:three_prime_UTR 40.0%
AATATGTCTAGCTGTGGTTG+TGG + chr4_4:13253464-13253483 Msa0670660:exon 40.0%
GAACCCAACCAAAAGAATCA+TGG - chr4_4:13253635-13253654 None:intergenic 40.0%
AATCACGGTTTCTGTGGTTT+CGG - chr4_4:13254231-13254250 None:intergenic 40.0%
! AACCACAGAAACCGTGATTT+TGG + chr4_4:13254232-13254251 Msa0670660:CDS 40.0%
! ACCACAGAAACCGTGATTTT+GGG + chr4_4:13254233-13254252 Msa0670660:CDS 40.0%
AGCACCTTCAAATTGGATCT+TGG - chr4_4:13254270-13254289 None:intergenic 40.0%
CCATTTCAGCACCTTCAAAT+TGG - chr4_4:13254277-13254296 None:intergenic 40.0%
!! CCAATTTGAAGGTGCTGAAA+TGG + chr4_4:13254274-13254293 Msa0670660:CDS 40.0%
CACTTCATTTGCAGTTGCAA+GGG - chr4_4:13254352-13254371 None:intergenic 40.0%
AGCTATGATCGCTTTGTGTT+TGG + chr4_4:13254555-13254574 Msa0670660:three_prime_UTR 40.0%
CGGCATTTATCGACATAAGT+TGG + chr4_4:13254697-13254716 Msa0670660:three_prime_UTR 40.0%
TGTGGTGATAGCTGCAAGTA+AGG + chr4_4:13253500-13253519 Msa0670660:intron 45.0%
! AGATATGCACAACGACTTGG+TGG + chr4_4:13254004-13254023 Msa0670660:intron 45.0%
!! GATATGCACAACGACTTGGT+GGG + chr4_4:13254005-13254024 Msa0670660:intron 45.0%
TACTTTGCAGATGCAGCAAG+AGG + chr4_4:13254180-13254199 Msa0670660:intron 45.0%
CCTTCAAATTGGATCTTGGC+CGG - chr4_4:13254266-13254285 None:intergenic 45.0%
GCACTTCATTTGCAGTTGCA+AGG - chr4_4:13254353-13254372 None:intergenic 45.0%
TCCACTATCTCTGCTTCAAG+AGG - chr4_4:13254392-13254411 None:intergenic 45.0%
TGTGGTGATAGCTGCAAGTA+AGG + chr4_4:13253500-13253519 Msa0670660:intron 45.0%
! AGATATGCACAACGACTTGG+TGG + chr4_4:13254004-13254023 Msa0670660:intron 45.0%
!! GATATGCACAACGACTTGGT+GGG + chr4_4:13254005-13254024 Msa0670660:intron 45.0%
TACTTTGCAGATGCAGCAAG+AGG + chr4_4:13254180-13254199 Msa0670660:intron 45.0%
CCTTCAAATTGGATCTTGGC+CGG - chr4_4:13254266-13254285 None:intergenic 45.0%
GCACTTCATTTGCAGTTGCA+AGG - chr4_4:13254353-13254372 None:intergenic 45.0%
TCCACTATCTCTGCTTCAAG+AGG - chr4_4:13254392-13254411 None:intergenic 45.0%
TGTGGAAGCAGTTGCAACTG+TGG + chr4_4:13253482-13253501 Msa0670660:CDS 50.0%
GTGGGTACTCCTCAAGTCAA+TGG + chr4_4:13254023-13254042 Msa0670660:intron 50.0%
AGATGCAGCAAGAGGTCTAG+TGG + chr4_4:13254188-13254207 Msa0670660:intron 50.0%
!! TAGTGGATTGAGCTATGCCG+AGG + chr4_4:13254205-13254224 Msa0670660:CDS 50.0%
GCCCAAAATCACGGTTTCTG+TGG - chr4_4:13254237-13254256 None:intergenic 50.0%
! GAAACCGTGATTTTGGGCGT+TGG + chr4_4:13254239-13254258 Msa0670660:CDS 50.0%
CCGGCCAAGATCCAATTTGA+AGG + chr4_4:13254263-13254282 Msa0670660:CDS 50.0%
! TGAAATGGGTGTTGCAGCTG+AGG + chr4_4:13254289-13254308 Msa0670660:CDS 50.0%
GCCTCTTGAAGCAGAGATAG+TGG + chr4_4:13254388-13254407 Msa0670660:three_prime_UTR 50.0%
TGTGGAAGCAGTTGCAACTG+TGG + chr4_4:13253482-13253501 Msa0670660:CDS 50.0%
GTGGGTACTCCTCAAGTCAA+TGG + chr4_4:13254023-13254042 Msa0670660:intron 50.0%
AGATGCAGCAAGAGGTCTAG+TGG + chr4_4:13254188-13254207 Msa0670660:intron 50.0%
!! TAGTGGATTGAGCTATGCCG+AGG + chr4_4:13254205-13254224 Msa0670660:CDS 50.0%
GCCCAAAATCACGGTTTCTG+TGG - chr4_4:13254237-13254256 None:intergenic 50.0%
! GAAACCGTGATTTTGGGCGT+TGG + chr4_4:13254239-13254258 Msa0670660:CDS 50.0%
CCGGCCAAGATCCAATTTGA+AGG + chr4_4:13254263-13254282 Msa0670660:CDS 50.0%
! TGAAATGGGTGTTGCAGCTG+AGG + chr4_4:13254289-13254308 Msa0670660:CDS 50.0%
GCCTCTTGAAGCAGAGATAG+TGG + chr4_4:13254388-13254407 Msa0670660:three_prime_UTR 50.0%
GGTTTCTGTGGTTTCGGCCT+CGG - chr4_4:13254225-13254244 None:intergenic 55.0%
CGGACCAACGCCCAAAATCA+CGG - chr4_4:13254246-13254265 None:intergenic 55.0%
!!! CGTGATTTTGGGCGTTGGTC+CGG + chr4_4:13254244-13254263 Msa0670660:CDS 55.0%
! ATGGGTGTTGCAGCTGAGGA+TGG + chr4_4:13254293-13254312 Msa0670660:CDS 55.0%
GGTTTCTGTGGTTTCGGCCT+CGG - chr4_4:13254225-13254244 None:intergenic 55.0%
CGGACCAACGCCCAAAATCA+CGG - chr4_4:13254246-13254265 None:intergenic 55.0%
!!! CGTGATTTTGGGCGTTGGTC+CGG + chr4_4:13254244-13254263 Msa0670660:CDS 55.0%
! ATGGGTGTTGCAGCTGAGGA+TGG + chr4_4:13254293-13254312 Msa0670660:CDS 55.0%
! GGTGTTGCAGCTGAGGATGG+TGG + chr4_4:13254296-13254315 Msa0670660:CDS 60.0%
! GAGGATGGTGGCTGCAAGTG+TGG + chr4_4:13254308-13254327 Msa0670660:CDS 60.0%
! GGTGTTGCAGCTGAGGATGG+TGG + chr4_4:13254296-13254315 Msa0670660:CDS 60.0%
! GAGGATGGTGGCTGCAAGTG+TGG + chr4_4:13254308-13254327 Msa0670660:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 13253382 13254721 13253382 ID=Msa0670660;Name=Msa0670660
chr4_4 mRNA 13253382 13254721 13253382 ID=Msa0670660-mRNA-1;Parent=Msa0670660;Name=Msa0670660-mRNA-1;_AED=0.23;_eAED=0.23;_QI=85|1|1|1|1|1|2|354|75
chr4_4 exon 13253382 13253516 13253382 ID=Msa0670660-mRNA-1:exon:4034;Parent=Msa0670660-mRNA-1
chr4_4 exon 13254190 13254721 13254190 ID=Msa0670660-mRNA-1:exon:4035;Parent=Msa0670660-mRNA-1
chr4_4 five_prime_UTR 13253382 13253466 13253382 ID=Msa0670660-mRNA-1:five_prime_utr;Parent=Msa0670660-mRNA-1
chr4_4 CDS 13253467 13253516 13253467 ID=Msa0670660-mRNA-1:cds;Parent=Msa0670660-mRNA-1
chr4_4 CDS 13254190 13254367 13254190 ID=Msa0670660-mRNA-1:cds;Parent=Msa0670660-mRNA-1
chr4_4 three_prime_UTR 13254368 13254721 13254368 ID=Msa0670660-mRNA-1:three_prime_utr;Parent=Msa0670660-mRNA-1
Gene Sequence

>Msa0670660

ATGTCTAGCTGTGGTTGTGGAAGCAGTTGCAACTGTGGTGATAGCTGCAAATGCAGCAAGAGGTCTAGTGGATTGAGCTATGCCGAGGCCGAAACCACAGAAACCGTGATTTTGGGCGTTGGTCCGGCCAAGATCCAATTTGAAGGTGCTGAAATGGGTGTTGCAGCTGAGGATGGTGGCTGCAAGTGTGGAGATAACTGCACATGTGACCCTTGCAACTGCAAATGA

Protein sequence

>Msa0670660

MSSCGCGSSCNCGDSCKCSKRSSGLSYAEAETTETVILGVGPAKIQFEGAEMGVAAEDGGCKCGDNCTCDPCNCK*