Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0017510 | Msa0738520 | 0.811259 | 7.657083e-51 | -8.615850e-47 |
| Msa0025330 | Msa0738520 | 0.800645 | 1.307605e-48 | -8.615850e-47 |
| Msa0738520 | Msa0770720 | 0.803430 | 3.499432e-49 | -8.615850e-47 |
| Msa0738520 | Msa0824660 | 0.805177 | 1.513907e-49 | -8.615850e-47 |
| Msa0738520 | Msa0828560 | 0.809192 | 2.136877e-50 | -8.615850e-47 |
| Msa0738520 | Msa0858840 | 0.805903 | 1.066354e-49 | -8.615850e-47 |
| Msa0738520 | Msa0866520 | 0.801557 | 8.511531e-49 | -8.615850e-47 |
| Msa0738520 | Msa0880140 | 0.808539 | 2.947372e-50 | -8.615850e-47 |
| Msa0738520 | Msa1045200 | 0.800765 | 1.236236e-48 | -8.615850e-47 |
| Msa0738520 | Msa1085200 | 0.818764 | 1.652920e-52 | -8.615850e-47 |
| Msa0738520 | Msa1140210 | 0.804819 | 1.798386e-49 | -8.615850e-47 |
| Msa0738520 | Msa1252140 | 0.807384 | 5.188555e-50 | -8.615850e-47 |
| Msa0738520 | Msa1353980 | 0.800827 | 1.200721e-48 | -8.615850e-47 |
| Msa0738520 | Msa1398340 | 0.801960 | 7.035870e-49 | -8.615850e-47 |
| Msa0738520 | Msa1460020 | 0.811725 | 6.065318e-51 | -8.615850e-47 |
| Msa0738520 | Msa1464810 | 0.807717 | 4.410609e-50 | -8.615850e-47 |
| Msa0157290 | Msa0738520 | 0.811848 | 5.700848e-51 | -8.615850e-47 |
| Msa0366240 | Msa0738520 | 0.823611 | 1.261577e-53 | -8.615850e-47 |
| Msa0410650 | Msa0738520 | 0.815824 | 7.583965e-52 | -8.615850e-47 |
| Msa0258310 | Msa0738520 | 0.803301 | 3.721742e-49 | -8.615850e-47 |
| Msa0262510 | Msa0738520 | 0.821689 | 3.533099e-53 | -8.615850e-47 |
| Msa0284420 | Msa0738520 | 0.804097 | 2.544452e-49 | -8.615850e-47 |
| Msa0507680 | Msa0738520 | 0.819414 | 1.176348e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCCTCATTTCATAATCT+TGG | 0.259074 | 5_1:-56270299 | None:intergenic |
| CATTCTTTGAAGAAGCAAAA+TGG | 0.312978 | 5_1:-56270845 | None:intergenic |
| AAAACTAACAATTTGTTAGT+TGG | 0.351135 | 5_1:-56270168 | None:intergenic |
| CCTTTCTTGCAAGAAATACT+TGG | 0.363433 | 5_1:-56270365 | None:intergenic |
| TTCAAATGCTCCCCTGACTT+TGG | 0.366185 | 5_1:+56270804 | Msa0738520:CDS |
| TGTTGTGTCCTTATCAAGAT+TGG | 0.368222 | 5_1:+56270061 | None:intergenic |
| AAATACTTGGTCCGACATAT+TGG | 0.368493 | 5_1:-56270352 | None:intergenic |
| AAGCAAAATGGTTTGCTTCT+CGG | 0.368539 | 5_1:-56270833 | None:intergenic |
| CCAAGTATTTCTTGCAAGAA+AGG | 0.377212 | 5_1:+56270365 | Msa0738520:CDS |
| TCTCGGAATGTTCCAAAGTC+AGG | 0.408425 | 5_1:-56270816 | None:intergenic |
| CCTCATTTCATAATCTTGGT+TGG | 0.436906 | 5_1:-56270295 | None:intergenic |
| TCTTCCTCATGAGTCATTGA+AGG | 0.474531 | 5_1:-56270420 | None:intergenic |
| TGGTGGTGGAGAAGAGATAT+AGG | 0.485148 | 5_1:-56270115 | None:intergenic |
| ATGTGCAAGAATATATATAG+AGG | 0.485343 | 5_1:-56270218 | None:intergenic |
| TAGTGGATCCAATCTTGATA+AGG | 0.509800 | 5_1:-56270069 | None:intergenic |
| TTGATTGGGTGCGATGGTGG+TGG | 0.514450 | 5_1:-56270129 | None:intergenic |
| TTCAATGACTCATGAGGAAG+AGG | 0.520203 | 5_1:+56270422 | Msa0738520:CDS |
| CACAATGATTTCATCAGTAG+TGG | 0.524252 | 5_1:-56270086 | None:intergenic |
| CTCGGAATGTTCCAAAGTCA+GGG | 0.545178 | 5_1:-56270815 | None:intergenic |
| ATTTCTTGCAAGAAAGGCAA+AGG | 0.564591 | 5_1:+56270371 | Msa0738520:CDS |
| CCAACCAAGATTATGAAATG+AGG | 0.575459 | 5_1:+56270295 | Msa0738520:exon |
| TGATCCTTCAATGACTCATG+AGG | 0.582376 | 5_1:+56270416 | Msa0738520:CDS |
| ATATACTCTAACCAATATGT+CGG | 0.607580 | 5_1:+56270341 | Msa0738520:CDS |
| CACAAGCATCACGTCTACCT+CGG | 0.613783 | 5_1:+56270762 | Msa0738520:CDS |
| TTGCAAGAAAGGCAAAGGTG+TGG | 0.616875 | 5_1:+56270376 | Msa0738520:CDS |
| TCGGAATGTTCCAAAGTCAG+GGG | 0.676801 | 5_1:-56270814 | None:intergenic |
| ATGCACGACAGTGAGCACCG+AGG | 0.838905 | 5_1:-56270779 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTGATTTGAATTTTGATT+GGG | - | chr5_1:56270146-56270165 | None:intergenic | 15.0% |
| !! | ATTAAAAAAAGAAAAAGAAG+TGG | - | chr5_1:56270263-56270282 | None:intergenic | 15.0% |
| !!! | TTTGAAATTTTTTAAGTTGA+GGG | + | chr5_1:56270574-56270593 | Msa0738520:intron | 15.0% |
| !!! | ATTTGATTTGAATTTTGATT+GGG | - | chr5_1:56270146-56270165 | None:intergenic | 15.0% |
| !! | ATTAAAAAAAGAAAAAGAAG+TGG | - | chr5_1:56270263-56270282 | None:intergenic | 15.0% |
| !!! | TTTGAAATTTTTTAAGTTGA+GGG | + | chr5_1:56270574-56270593 | Msa0738520:intron | 15.0% |
| !!! | GATTTGATTTGAATTTTGAT+TGG | - | chr5_1:56270147-56270166 | None:intergenic | 20.0% |
| !!! | AAAACTAACAATTTGTTAGT+TGG | - | chr5_1:56270171-56270190 | None:intergenic | 20.0% |
| !!! | CTTTGAAATTTTTTAAGTTG+AGG | + | chr5_1:56270573-56270592 | Msa0738520:intron | 20.0% |
| !! | AATAATAAATACCTGAAACA+AGG | - | chr5_1:56270723-56270742 | None:intergenic | 20.0% |
| !! | TTATTATTAAATTCGTCTGA+AGG | + | chr5_1:56270735-56270754 | Msa0738520:intron | 20.0% |
| !!! | GATTTGATTTGAATTTTGAT+TGG | - | chr5_1:56270147-56270166 | None:intergenic | 20.0% |
| !!! | AAAACTAACAATTTGTTAGT+TGG | - | chr5_1:56270171-56270190 | None:intergenic | 20.0% |
| !!! | CTTTGAAATTTTTTAAGTTG+AGG | + | chr5_1:56270573-56270592 | Msa0738520:intron | 20.0% |
| !! | AATAATAAATACCTGAAACA+AGG | - | chr5_1:56270723-56270742 | None:intergenic | 20.0% |
| !! | TTATTATTAAATTCGTCTGA+AGG | + | chr5_1:56270735-56270754 | Msa0738520:intron | 20.0% |
| ! | ATGTGCAAGAATATATATAG+AGG | - | chr5_1:56270221-56270240 | None:intergenic | 25.0% |
| ! | ATATACTCTAACCAATATGT+CGG | + | chr5_1:56270341-56270360 | Msa0738520:CDS | 25.0% |
| ! | ATGTGCAAGAATATATATAG+AGG | - | chr5_1:56270221-56270240 | None:intergenic | 25.0% |
| ! | ATATACTCTAACCAATATGT+CGG | + | chr5_1:56270341-56270360 | Msa0738520:CDS | 25.0% |
| TCTTCCTCATTTCATAATCT+TGG | - | chr5_1:56270302-56270321 | None:intergenic | 30.0% | |
| TCTATTCTCATTATGTTGCT+TGG | + | chr5_1:56270456-56270475 | Msa0738520:intron | 30.0% | |
| !!! | ATTAGTCTTTTGTCTTCCAA+GGG | + | chr5_1:56270620-56270639 | Msa0738520:intron | 30.0% |
| TTGATAAGATGACGAACTAA+TGG | - | chr5_1:56270695-56270714 | None:intergenic | 30.0% | |
| !!! | TATCAATTTTGCCTTGTTTC+AGG | + | chr5_1:56270709-56270728 | Msa0738520:intron | 30.0% |
| ! | CATTCTTTGAAGAAGCAAAA+TGG | - | chr5_1:56270848-56270867 | None:intergenic | 30.0% |
| TCTTCCTCATTTCATAATCT+TGG | - | chr5_1:56270302-56270321 | None:intergenic | 30.0% | |
| TCTATTCTCATTATGTTGCT+TGG | + | chr5_1:56270456-56270475 | Msa0738520:intron | 30.0% | |
| !!! | ATTAGTCTTTTGTCTTCCAA+GGG | + | chr5_1:56270620-56270639 | Msa0738520:intron | 30.0% |
| TTGATAAGATGACGAACTAA+TGG | - | chr5_1:56270695-56270714 | None:intergenic | 30.0% | |
| !!! | TATCAATTTTGCCTTGTTTC+AGG | + | chr5_1:56270709-56270728 | Msa0738520:intron | 30.0% |
| ! | CATTCTTTGAAGAAGCAAAA+TGG | - | chr5_1:56270848-56270867 | None:intergenic | 30.0% |
| ! | TAGTGGATCCAATCTTGATA+AGG | - | chr5_1:56270072-56270091 | None:intergenic | 35.0% |
| CACAATGATTTCATCAGTAG+TGG | - | chr5_1:56270089-56270108 | None:intergenic | 35.0% | |
| CCTCATTTCATAATCTTGGT+TGG | - | chr5_1:56270298-56270317 | None:intergenic | 35.0% | |
| CCAACCAAGATTATGAAATG+AGG | + | chr5_1:56270295-56270314 | Msa0738520:exon | 35.0% | |
| AAATACTTGGTCCGACATAT+TGG | - | chr5_1:56270355-56270374 | None:intergenic | 35.0% | |
| CCTTTCTTGCAAGAAATACT+TGG | - | chr5_1:56270368-56270387 | None:intergenic | 35.0% | |
| CCAAGTATTTCTTGCAAGAA+AGG | + | chr5_1:56270365-56270384 | Msa0738520:CDS | 35.0% | |
| ATTTCTTGCAAGAAAGGCAA+AGG | + | chr5_1:56270371-56270390 | Msa0738520:CDS | 35.0% | |
| !!! | TAAGTTGAGGGTTTTGTTGA+TGG | + | chr5_1:56270586-56270605 | Msa0738520:intron | 35.0% |
| !!! | CATTAGTCTTTTGTCTTCCA+AGG | + | chr5_1:56270619-56270638 | Msa0738520:intron | 35.0% |
| ATAAGATGACGAACTAATGG+AGG | - | chr5_1:56270692-56270711 | None:intergenic | 35.0% | |
| !! | AAGCAAAATGGTTTGCTTCT+CGG | - | chr5_1:56270836-56270855 | None:intergenic | 35.0% |
| ! | TAGTGGATCCAATCTTGATA+AGG | - | chr5_1:56270072-56270091 | None:intergenic | 35.0% |
| CACAATGATTTCATCAGTAG+TGG | - | chr5_1:56270089-56270108 | None:intergenic | 35.0% | |
| CCTCATTTCATAATCTTGGT+TGG | - | chr5_1:56270298-56270317 | None:intergenic | 35.0% | |
| CCAACCAAGATTATGAAATG+AGG | + | chr5_1:56270295-56270314 | Msa0738520:exon | 35.0% | |
| AAATACTTGGTCCGACATAT+TGG | - | chr5_1:56270355-56270374 | None:intergenic | 35.0% | |
| CCTTTCTTGCAAGAAATACT+TGG | - | chr5_1:56270368-56270387 | None:intergenic | 35.0% | |
| CCAAGTATTTCTTGCAAGAA+AGG | + | chr5_1:56270365-56270384 | Msa0738520:CDS | 35.0% | |
| ATTTCTTGCAAGAAAGGCAA+AGG | + | chr5_1:56270371-56270390 | Msa0738520:CDS | 35.0% | |
| !!! | TAAGTTGAGGGTTTTGTTGA+TGG | + | chr5_1:56270586-56270605 | Msa0738520:intron | 35.0% |
| !!! | CATTAGTCTTTTGTCTTCCA+AGG | + | chr5_1:56270619-56270638 | Msa0738520:intron | 35.0% |
| ATAAGATGACGAACTAATGG+AGG | - | chr5_1:56270692-56270711 | None:intergenic | 35.0% | |
| !! | AAGCAAAATGGTTTGCTTCT+CGG | - | chr5_1:56270836-56270855 | None:intergenic | 35.0% |
| !!! | TGAATTTTGATTGGGTGCGA+TGG | - | chr5_1:56270138-56270157 | None:intergenic | 40.0% |
| TGATCCTTCAATGACTCATG+AGG | + | chr5_1:56270416-56270435 | Msa0738520:CDS | 40.0% | |
| TCTTCCTCATGAGTCATTGA+AGG | - | chr5_1:56270423-56270442 | None:intergenic | 40.0% | |
| TTCAATGACTCATGAGGAAG+AGG | + | chr5_1:56270422-56270441 | Msa0738520:CDS | 40.0% | |
| ! | TTATGTTGCTTGGTTGCATG+TGG | + | chr5_1:56270466-56270485 | Msa0738520:intron | 40.0% |
| TTTGTCTGAGCCTTGAACAA+GGG | - | chr5_1:56270650-56270669 | None:intergenic | 40.0% | |
| ATTTGTCTGAGCCTTGAACA+AGG | - | chr5_1:56270651-56270670 | None:intergenic | 40.0% | |
| !!! | TGAATTTTGATTGGGTGCGA+TGG | - | chr5_1:56270138-56270157 | None:intergenic | 40.0% |
| TGATCCTTCAATGACTCATG+AGG | + | chr5_1:56270416-56270435 | Msa0738520:CDS | 40.0% | |
| TCTTCCTCATGAGTCATTGA+AGG | - | chr5_1:56270423-56270442 | None:intergenic | 40.0% | |
| TTCAATGACTCATGAGGAAG+AGG | + | chr5_1:56270422-56270441 | Msa0738520:CDS | 40.0% | |
| ! | TTATGTTGCTTGGTTGCATG+TGG | + | chr5_1:56270466-56270485 | Msa0738520:intron | 40.0% |
| TTTGTCTGAGCCTTGAACAA+GGG | - | chr5_1:56270650-56270669 | None:intergenic | 40.0% | |
| ATTTGTCTGAGCCTTGAACA+AGG | - | chr5_1:56270651-56270670 | None:intergenic | 40.0% | |
| !! | TGGTGGTGGAGAAGAGATAT+AGG | - | chr5_1:56270118-56270137 | None:intergenic | 45.0% |
| !!! | ATTTTGATTGGGTGCGATGG+TGG | - | chr5_1:56270135-56270154 | None:intergenic | 45.0% |
| TTGCAAGAAAGGCAAAGGTG+TGG | + | chr5_1:56270376-56270395 | Msa0738520:CDS | 45.0% | |
| TTGTCTGAGCCTTGAACAAG+GGG | - | chr5_1:56270649-56270668 | None:intergenic | 45.0% | |
| TTCAAATGCTCCCCTGACTT+TGG | + | chr5_1:56270804-56270823 | Msa0738520:CDS | 45.0% | |
| TCGGAATGTTCCAAAGTCAG+GGG | - | chr5_1:56270817-56270836 | None:intergenic | 45.0% | |
| CTCGGAATGTTCCAAAGTCA+GGG | - | chr5_1:56270818-56270837 | None:intergenic | 45.0% | |
| TCTCGGAATGTTCCAAAGTC+AGG | - | chr5_1:56270819-56270838 | None:intergenic | 45.0% | |
| !! | TGGTGGTGGAGAAGAGATAT+AGG | - | chr5_1:56270118-56270137 | None:intergenic | 45.0% |
| !!! | ATTTTGATTGGGTGCGATGG+TGG | - | chr5_1:56270135-56270154 | None:intergenic | 45.0% |
| TTGCAAGAAAGGCAAAGGTG+TGG | + | chr5_1:56270376-56270395 | Msa0738520:CDS | 45.0% | |
| TTGTCTGAGCCTTGAACAAG+GGG | - | chr5_1:56270649-56270668 | None:intergenic | 45.0% | |
| TTCAAATGCTCCCCTGACTT+TGG | + | chr5_1:56270804-56270823 | Msa0738520:CDS | 45.0% | |
| TCGGAATGTTCCAAAGTCAG+GGG | - | chr5_1:56270817-56270836 | None:intergenic | 45.0% | |
| CTCGGAATGTTCCAAAGTCA+GGG | - | chr5_1:56270818-56270837 | None:intergenic | 45.0% | |
| TCTCGGAATGTTCCAAAGTC+AGG | - | chr5_1:56270819-56270838 | None:intergenic | 45.0% | |
| !! | AAAAAAAATAAAAAAATGAA+AGG | - | chr5_1:56270505-56270524 | None:intergenic | 5.0% |
| !!! | TTCATTTTTTTATTTTTTTT+TGG | + | chr5_1:56270505-56270524 | Msa0738520:intron | 5.0% |
| !! | AAAAAAAATAAAAAAATGAA+AGG | - | chr5_1:56270505-56270524 | None:intergenic | 5.0% |
| !!! | TTCATTTTTTTATTTTTTTT+TGG | + | chr5_1:56270505-56270524 | Msa0738520:intron | 5.0% |
| CTTGAACAAGGGGAATCCCT+TGG | - | chr5_1:56270639-56270658 | None:intergenic | 50.0% | |
| CAAGGGATTCCCCTTGTTCA+AGG | + | chr5_1:56270637-56270656 | Msa0738520:intron | 50.0% | |
| CACAAGCATCACGTCTACCT+CGG | + | chr5_1:56270762-56270781 | Msa0738520:CDS | 50.0% | |
| CTTGAACAAGGGGAATCCCT+TGG | - | chr5_1:56270639-56270658 | None:intergenic | 50.0% | |
| CAAGGGATTCCCCTTGTTCA+AGG | + | chr5_1:56270637-56270656 | Msa0738520:intron | 50.0% | |
| CACAAGCATCACGTCTACCT+CGG | + | chr5_1:56270762-56270781 | Msa0738520:CDS | 50.0% | |
| ! | TTGATTGGGTGCGATGGTGG+TGG | - | chr5_1:56270132-56270151 | None:intergenic | 55.0% |
| ! | TTGATTGGGTGCGATGGTGG+TGG | - | chr5_1:56270132-56270151 | None:intergenic | 55.0% |
| ! | ATGCACGACAGTGAGCACCG+AGG | - | chr5_1:56270782-56270801 | None:intergenic | 60.0% |
| ! | ATGCACGACAGTGAGCACCG+AGG | - | chr5_1:56270782-56270801 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 56270066 | 56270873 | 56270066 | ID=Msa0738520;Name=Msa0738520 |
| chr5_1 | mRNA | 56270066 | 56270873 | 56270066 | ID=Msa0738520-mRNA-1;Parent=Msa0738520;Name=Msa0738520-mRNA-1;_AED=0.17;_eAED=0.36;_QI=246|0|0.5|1|1|1|2|0|82 |
| chr5_1 | exon | 56270066 | 56270443 | 56270066 | ID=Msa0738520-mRNA-1:exon:15953;Parent=Msa0738520-mRNA-1 |
| chr5_1 | exon | 56270757 | 56270873 | 56270757 | ID=Msa0738520-mRNA-1:exon:15954;Parent=Msa0738520-mRNA-1 |
| chr5_1 | five_prime_UTR | 56270066 | 56270311 | 56270066 | ID=Msa0738520-mRNA-1:five_prime_utr;Parent=Msa0738520-mRNA-1 |
| chr5_1 | CDS | 56270312 | 56270443 | 56270312 | ID=Msa0738520-mRNA-1:cds;Parent=Msa0738520-mRNA-1 |
| chr5_1 | CDS | 56270757 | 56270873 | 56270757 | ID=Msa0738520-mRNA-1:cds;Parent=Msa0738520-mRNA-1 |
| Gene Sequence |
| Protein sequence |
