AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0609970


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0609970 MtrunA17_Chr4g0042961 91.200 125 8 1 1 122 1 125 5.57e-68 200
Msa0609970 MtrunA17_Chr4g0042951 66.667 132 31 4 3 122 11 141 1.75e-40 131
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0609970 AT2G03440.1 42.636 129 52 5 11 122 64 187 4.74e-14 65.5
Msa0609970 AT1G13930.3 48.062 129 47 8 8 122 33 155 2.28e-13 63.2
Msa0609970 AT1G13930.1 48.062 129 47 8 8 122 33 155 2.28e-13 63.2
Msa0609970 AT1G13930.2 48.062 129 47 8 8 122 33 155 2.28e-13 63.2

Find 25 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTTCAACCAGTGAGCTTA+TGG 0.299153 4_2:+57567181 Msa0609970:CDS
AGGCAGCACAGTCAGGTTTC+GGG 0.304942 4_2:+57567225 Msa0609970:CDS
GAGGCAGCACAGTCAGGTTT+CGG 0.309235 4_2:+57567224 Msa0609970:CDS
CCACCTGATCCTCCACCTTC+AGG 0.341637 4_2:-57567455 None:intergenic
AGTTGGTCAGTATGCTAAAT+TGG 0.398975 4_2:+57567301 Msa0609970:CDS
AAGCCTGAAGGTGGAGGATC+AGG 0.423090 4_2:+57567452 Msa0609970:CDS
TGCACTTGCCATAAGCTCAC+TGG 0.466727 4_2:-57567189 None:intergenic
AGCTGCTGATTATCTGCATA+AGG 0.469713 4_2:+57567358 Msa0609970:CDS
GGTGGAGGATCAGGTGGACT+AGG 0.476662 4_2:+57567461 Msa0609970:CDS
GACAAGACAGCAGAAGCTGC+AGG 0.506059 4_2:+57567263 Msa0609970:CDS
GTAGCAGAGGCAGCACAGTC+AGG 0.527765 4_2:+57567218 Msa0609970:CDS
TGATCTGCAGGTTTAGAAGC+TGG 0.532370 4_2:-57567407 None:intergenic
AAGGTTGAGAACACTACTAC+TGG 0.547092 4_2:+57567377 Msa0609970:CDS
AAGTGCAAAGGTGGTAGCAG+AGG 0.549500 4_2:+57567205 Msa0609970:CDS
TTGGATGAACAGAAAGGTGT+AGG 0.558648 4_2:+57567320 Msa0609970:CDS
GCTAAATTGGATGAACAGAA+AGG 0.567227 4_2:+57567314 Msa0609970:CDS
CCTGAAGGTGGAGGATCAGG+TGG 0.601078 4_2:+57567455 Msa0609970:CDS
TGAGCTTATGGCAAGTGCAA+AGG 0.606848 4_2:+57567193 Msa0609970:CDS
GGAGGATCAGGTGGACTAGG+TGG 0.611533 4_2:+57567464 Msa0609970:CDS
GCTTATGGCAAGTGCAAAGG+TGG 0.622253 4_2:+57567196 Msa0609970:CDS
GGTGATCTTCTAGATGCAGT+TGG 0.625884 4_2:+57567284 Msa0609970:CDS
GTTGCTAAGCCTGAAGGTGG+AGG 0.637775 4_2:+57567446 Msa0609970:CDS
TCTGCAGGTTTAGAAGCTGG+TGG 0.662750 4_2:-57567404 None:intergenic
GATGATGTTGCTAAGCCTGA+AGG 0.667542 4_2:+57567440 Msa0609970:CDS
GATGTTGCTAAGCCTGAAGG+TGG 0.674394 4_2:+57567443 Msa0609970:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAGCAACATCATCACTTTT+AGG - chr4_2:57567434-57567453 None:intergenic 30.0%
AGTTGGTCAGTATGCTAAAT+TGG + chr4_2:57567301-57567320 Msa0609970:CDS 35.0%
GCTAAATTGGATGAACAGAA+AGG + chr4_2:57567314-57567333 Msa0609970:CDS 35.0%
GATTTCAACCAGTGAGCTTA+TGG + chr4_2:57567181-57567200 Msa0609970:CDS 40.0%
! TTGGATGAACAGAAAGGTGT+AGG + chr4_2:57567320-57567339 Msa0609970:CDS 40.0%
! AGCTGCTGATTATCTGCATA+AGG + chr4_2:57567358-57567377 Msa0609970:CDS 40.0%
AAGGTTGAGAACACTACTAC+TGG + chr4_2:57567377-57567396 Msa0609970:CDS 40.0%
! ACTAGGTGGTGATTTTGCTA+AGG + chr4_2:57567478-57567497 Msa0609970:CDS 40.0%
TGAGCTTATGGCAAGTGCAA+AGG + chr4_2:57567193-57567212 Msa0609970:CDS 45.0%
GGTGATCTTCTAGATGCAGT+TGG + chr4_2:57567284-57567303 Msa0609970:CDS 45.0%
TGATCTGCAGGTTTAGAAGC+TGG - chr4_2:57567410-57567429 None:intergenic 45.0%
!! TCACTTTTAGGCTGATCTGC+AGG - chr4_2:57567422-57567441 None:intergenic 45.0%
GATGATGTTGCTAAGCCTGA+AGG + chr4_2:57567440-57567459 Msa0609970:CDS 45.0%
! GGTGATTTTGCTAAGGTTGC+TGG + chr4_2:57567485-57567504 Msa0609970:CDS 45.0%
! GATTTTGCTAAGGTTGCTGG+AGG + chr4_2:57567488-57567507 Msa0609970:CDS 45.0%
TGCACTTGCCATAAGCTCAC+TGG - chr4_2:57567192-57567211 None:intergenic 50.0%
GCTTATGGCAAGTGCAAAGG+TGG + chr4_2:57567196-57567215 Msa0609970:CDS 50.0%
AAGTGCAAAGGTGGTAGCAG+AGG + chr4_2:57567205-57567224 Msa0609970:CDS 50.0%
TCTGCAGGTTTAGAAGCTGG+TGG - chr4_2:57567407-57567426 None:intergenic 50.0%
GATGTTGCTAAGCCTGAAGG+TGG + chr4_2:57567443-57567462 Msa0609970:CDS 50.0%
GAGGCAGCACAGTCAGGTTT+CGG + chr4_2:57567224-57567243 Msa0609970:CDS 55.0%
AGGCAGCACAGTCAGGTTTC+GGG + chr4_2:57567225-57567244 Msa0609970:CDS 55.0%
GACAAGACAGCAGAAGCTGC+AGG + chr4_2:57567263-57567282 Msa0609970:CDS 55.0%
GTTGCTAAGCCTGAAGGTGG+AGG + chr4_2:57567446-57567465 Msa0609970:CDS 55.0%
AAGCCTGAAGGTGGAGGATC+AGG + chr4_2:57567452-57567471 Msa0609970:CDS 55.0%
GTAGCAGAGGCAGCACAGTC+AGG + chr4_2:57567218-57567237 Msa0609970:CDS 60.0%
CCACCTGATCCTCCACCTTC+AGG - chr4_2:57567458-57567477 None:intergenic 60.0%
CCTGAAGGTGGAGGATCAGG+TGG + chr4_2:57567455-57567474 Msa0609970:CDS 60.0%
! GGTGGAGGATCAGGTGGACT+AGG + chr4_2:57567461-57567480 Msa0609970:CDS 60.0%
!! GGAGGATCAGGTGGACTAGG+TGG + chr4_2:57567464-57567483 Msa0609970:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 57567155 57567523 57567155 ID=Msa0609970;Name=Msa0609970
chr4_2 mRNA 57567155 57567523 57567155 ID=Msa0609970-mRNA-1;Parent=Msa0609970;Name=Msa0609970-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0|-1|0|1|-1|1|1|0|122
chr4_2 exon 57567155 57567523 57567155 ID=Msa0609970-mRNA-1:exon:14958;Parent=Msa0609970-mRNA-1
chr4_2 CDS 57567155 57567523 57567155 ID=Msa0609970-mRNA-1:cds;Parent=Msa0609970-mRNA-1
Gene Sequence

>Msa0609970

ATGGCAGCAAGTGGAGAAGAGAAGAAGATTTCAACCAGTGAGCTTATGGCAAGTGCAAAGGTGGTAGCAGAGGCAGCACAGTCAGGTTTCGGGAAAGATTTAGACAAAGACAAGACAGCAGAAGCTGCAGGTGATCTTCTAGATGCAGTTGGTCAGTATGCTAAATTGGATGAACAGAAAGGTGTAGGATCATATGTTGATAAAGCTGCTGATTATCTGCATAAGGTTGAGAACACTACTACTGGTGCTCCACCAGCTTCTAAACCTGCAGATCAGCCTAAAAGTGATGATGTTGCTAAGCCTGAAGGTGGAGGATCAGGTGGACTAGGTGGTGATTTTGCTAAGGTTGCTGGAGGTTTCTTTAAATGA

Protein sequence

>Msa0609970

MAASGEEKKISTSELMASAKVVAEAAQSGFGKDLDKDKTAEAAGDLLDAVGQYAKLDEQKGVGSYVDKAADYLHKVENTTTGAPPASKPADQPKSDDVAKPEGGGSGGLGGDFAKVAGGFFK*