Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0650620 | XP_003607781.1 | 91.200 | 125 | 8 | 1 | 1 | 122 | 1 | 125 | 6.06e-64 | 200 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0650620 | sp|Q9ZQ80|NDRP1_ARATH | 42.636 | 129 | 52 | 5 | 11 | 122 | 64 | 187 | 4.67e-13 | 65.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000490 | Msa0650620 | -0.830878 | 2.291967e-55 | -8.615850e-47 |
| Msa0032610 | Msa0650620 | 0.816113 | 6.535665e-52 | -8.615850e-47 |
| Msa0038310 | Msa0650620 | 0.813228 | 2.846329e-51 | -8.615850e-47 |
| Msa0050730 | Msa0650620 | 0.828761 | 7.509239e-55 | -8.615850e-47 |
| Msa0050760 | Msa0650620 | 0.807778 | 4.280626e-50 | -8.615850e-47 |
| Msa0072170 | Msa0650620 | 0.829210 | 5.847010e-55 | -8.615850e-47 |
| Msa0075340 | Msa0650620 | 0.800968 | 1.123549e-48 | -8.615850e-47 |
| Msa0086090 | Msa0650620 | 0.814162 | 1.773007e-51 | -8.615850e-47 |
| Msa0090030 | Msa0650620 | 0.834754 | 2.495967e-56 | -8.615850e-47 |
| Msa0096940 | Msa0650620 | -0.806084 | 9.767127e-50 | -8.615850e-47 |
| Msa0099510 | Msa0650620 | 0.812372 | 4.381565e-51 | -8.615850e-47 |
| Msa0120540 | Msa0650620 | 0.840327 | 9.294307e-58 | -8.615850e-47 |
| Msa0607290 | Msa0650620 | -0.833439 | 5.328890e-56 | -8.615850e-47 |
| Msa0607510 | Msa0650620 | 0.816223 | 6.178980e-52 | -8.615850e-47 |
| Msa0609970 | Msa0650620 | 0.970167 | 3.303463e-131 | -8.615850e-47 |
| Msa0614990 | Msa0650620 | 0.900064 | 1.064058e-77 | -8.615850e-47 |
| Msa0622340 | Msa0650620 | 0.853369 | 2.501079e-61 | -8.615850e-47 |
| Msa0650620 | Msa0650650 | 0.839641 | 1.402869e-57 | -8.615850e-47 |
| Msa0650620 | Msa0650730 | 0.831485 | 1.625036e-55 | -8.615850e-47 |
| Msa0650620 | Msa0650740 | 0.850121 | 2.084294e-60 | -8.615850e-47 |
| Msa0650620 | Msa0651320 | 0.817887 | 2.611781e-52 | -8.615850e-47 |
| Msa0650620 | Msa0659140 | 0.851921 | 6.478036e-61 | -8.615850e-47 |
| Msa0650620 | Msa0664760 | 0.815181 | 1.054195e-51 | -8.615850e-47 |
| Msa0650620 | Msa0669850 | 0.816022 | 6.849972e-52 | -8.615850e-47 |
| Msa0650620 | Msa0692110 | 0.825924 | 3.594628e-54 | -8.615850e-47 |
| Msa0650620 | Msa0694250 | 0.808858 | 2.519397e-50 | -8.615850e-47 |
| Msa0650620 | Msa0705600 | 0.841005 | 6.177130e-58 | -8.615850e-47 |
| Msa0650620 | Msa0711490 | 0.813786 | 2.146354e-51 | -8.615850e-47 |
| Msa0650620 | Msa0713460 | 0.814044 | 1.882722e-51 | -8.615850e-47 |
| Msa0650620 | Msa0738850 | 0.818592 | 1.808632e-52 | -8.615850e-47 |
| Msa0650620 | Msa0738900 | 0.817202 | 3.727166e-52 | -8.615850e-47 |
| Msa0650620 | Msa0755080 | 0.815225 | 1.031197e-51 | -8.615850e-47 |
| Msa0650620 | Msa0792090 | 0.822769 | 1.983815e-53 | -8.615850e-47 |
| Msa0650620 | Msa0812660 | 0.806401 | 8.373648e-50 | -8.615850e-47 |
| Msa0650620 | Msa0817260 | 0.802498 | 5.451943e-49 | -8.615850e-47 |
| Msa0650620 | Msa0819390 | 0.816038 | 6.794717e-52 | -8.615850e-47 |
| Msa0650620 | Msa0834450 | 0.814493 | 1.497988e-51 | -8.615850e-47 |
| Msa0650620 | Msa0859500 | 0.804609 | 1.990207e-49 | -8.615850e-47 |
| Msa0650620 | Msa0874500 | 0.811499 | 6.789577e-51 | -8.615850e-47 |
| Msa0650620 | Msa0876860 | -0.831966 | 1.237052e-55 | -8.615850e-47 |
| Msa0650620 | Msa0891600 | -0.817646 | 2.960589e-52 | -8.615850e-47 |
| Msa0650620 | Msa0895310 | 0.800071 | 1.711404e-48 | -8.615850e-47 |
| Msa0650620 | Msa0898860 | 0.800441 | 1.439269e-48 | -8.615850e-47 |
| Msa0650620 | Msa0908170 | 0.825207 | 5.315591e-54 | -8.615850e-47 |
| Msa0650620 | Msa0922540 | 0.832006 | 1.209015e-55 | -8.615850e-47 |
| Msa0650620 | Msa0928030 | 0.811474 | 6.875439e-51 | -8.615850e-47 |
| Msa0650620 | Msa0942730 | 0.815797 | 7.689016e-52 | -8.615850e-47 |
| Msa0650620 | Msa0944730 | 0.851696 | 7.499014e-61 | -8.615850e-47 |
| Msa0650620 | Msa0946080 | 0.813946 | 1.978286e-51 | -8.615850e-47 |
| Msa0650620 | Msa0956520 | -0.820298 | 7.384671e-53 | -8.615850e-47 |
| Msa0650620 | Msa0958020 | 0.806434 | 8.241704e-50 | -8.615850e-47 |
| Msa0650620 | Msa0959010 | 0.815200 | 1.044298e-51 | -8.615850e-47 |
| Msa0650620 | Msa0982590 | 0.815331 | 9.765928e-52 | -8.615850e-47 |
| Msa0650620 | Msa0993530 | 0.816843 | 4.485899e-52 | -8.615850e-47 |
| Msa0650620 | Msa1002600 | -0.803750 | 3.003136e-49 | -8.615850e-47 |
| Msa0650620 | Msa1015060 | 0.823589 | 1.276830e-53 | -8.615850e-47 |
| Msa0650620 | Msa1036270 | 0.832499 | 9.132002e-56 | -8.615850e-47 |
| Msa0650620 | Msa1069660 | -0.825400 | 4.785009e-54 | -8.615850e-47 |
| Msa0650620 | Msa1076540 | 0.840902 | 6.574144e-58 | -8.615850e-47 |
| Msa0650620 | Msa1089840 | 0.806013 | 1.010662e-49 | -8.615850e-47 |
| Msa0650620 | Msa1092820 | 0.838793 | 2.327997e-57 | -8.615850e-47 |
| Msa0650620 | Msa1094430 | 0.834964 | 2.210702e-56 | -8.615850e-47 |
| Msa0650620 | Msa1126120 | -0.822978 | 1.773363e-53 | -8.615850e-47 |
| Msa0650620 | Msa1131420 | 0.802653 | 5.065308e-49 | -8.615850e-47 |
| Msa0650620 | Msa1171770 | -0.817349 | 3.454171e-52 | -8.615850e-47 |
| Msa0650620 | Msa1182630 | 0.819559 | 1.089841e-52 | -8.615850e-47 |
| Msa0650620 | Msa1182640 | 0.871261 | 7.820705e-67 | -8.615850e-47 |
| Msa0650620 | Msa1193950 | -0.811533 | 6.675233e-51 | -8.615850e-47 |
| Msa0650620 | Msa1218650 | 0.861480 | 9.961707e-64 | -8.615850e-47 |
| Msa0650620 | Msa1219110 | 0.815469 | 9.101050e-52 | -8.615850e-47 |
| Msa0650620 | Msa1229850 | 0.819865 | 9.279232e-53 | -8.615850e-47 |
| Msa0650620 | Msa1235020 | 0.831038 | 2.093925e-55 | -8.615850e-47 |
| Msa0650620 | Msa1236070 | -0.846850 | 1.676095e-59 | -8.615850e-47 |
| Msa0650620 | Msa1246370 | 0.802485 | 5.486083e-49 | -8.615850e-47 |
| Msa0650620 | Msa1246530 | -0.826492 | 2.633312e-54 | -8.615850e-47 |
| Msa0650620 | Msa1249920 | 0.803491 | 3.398553e-49 | -8.615850e-47 |
| Msa0650620 | Msa1272520 | 0.850039 | 2.196541e-60 | -8.615850e-47 |
| Msa0650620 | Msa1282920 | 0.820677 | 6.043327e-53 | -8.615850e-47 |
| Msa0650620 | Msa1287360 | 0.864178 | 1.466154e-64 | -8.615850e-47 |
| Msa0650620 | Msa1291350 | 0.803241 | 3.828534e-49 | -8.615850e-47 |
| Msa0650620 | Msa1300860 | 0.808241 | 3.410967e-50 | -8.615850e-47 |
| Msa0650620 | Msa1310660 | 0.870907 | 1.023248e-66 | -8.615850e-47 |
| Msa0650620 | Msa1338610 | 0.856960 | 2.259487e-62 | -8.615850e-47 |
| Msa0650620 | Msa1350690 | 0.862255 | 5.766800e-64 | -8.615850e-47 |
| Msa0650620 | Msa1360280 | 0.837993 | 3.743385e-57 | -8.615850e-47 |
| Msa0650620 | Msa1363700 | 0.836598 | 8.519483e-57 | -8.615850e-47 |
| Msa0650620 | Msa1367390 | -0.804859 | 1.764745e-49 | -8.615850e-47 |
| Msa0650620 | Msa1371090 | 0.818064 | 2.382127e-52 | -8.615850e-47 |
| Msa0650620 | Msa1373800 | 0.852558 | 4.266984e-61 | -8.615850e-47 |
| Msa0650620 | Msa1383480 | 0.827690 | 1.361118e-54 | -8.615850e-47 |
| Msa0650620 | Msa1398860 | 0.803727 | 3.035857e-49 | -8.615850e-47 |
| Msa0650620 | Msa1404720 | 0.836140 | 1.114354e-56 | -8.615850e-47 |
| Msa0650620 | Msa1405950 | 0.806554 | 7.776266e-50 | -8.615850e-47 |
| Msa0650620 | Msa1415920 | 0.808341 | 3.248381e-50 | -8.615850e-47 |
| Msa0650620 | Msa1417810 | -0.820268 | 7.503423e-53 | -8.615850e-47 |
| Msa0650620 | Msa1419110 | 0.845703 | 3.442704e-59 | -8.615850e-47 |
| Msa0650620 | Msa1439230 | 0.854364 | 1.293065e-61 | -8.615850e-47 |
| Msa0650620 | Msa1440600 | 0.809241 | 2.085741e-50 | -8.615850e-47 |
| Msa0650620 | Msa1442220 | -0.839883 | 1.214003e-57 | -8.615850e-47 |
| Msa0650620 | Msa1445400 | -0.823030 | 1.724879e-53 | -8.615850e-47 |
| Msa0650620 | Msa1449350 | -0.818952 | 1.498550e-52 | -8.615850e-47 |
| Msa0650620 | Msa1450980 | 0.802056 | 6.722901e-49 | -8.615850e-47 |
| Msa0650620 | Msa1452470 | 0.847296 | 1.265126e-59 | -8.615850e-47 |
| Msa0650620 | Msa1454750 | 0.802652 | 5.068339e-49 | -8.615850e-47 |
| Msa0650620 | Msa1459760 | -0.805157 | 1.528663e-49 | -8.615850e-47 |
| Msa0650620 | Msa1465960 | 0.808918 | 2.445970e-50 | -8.615850e-47 |
| Msa0132620 | Msa0650620 | 0.845268 | 4.513843e-59 | -8.615850e-47 |
| Msa0136390 | Msa0650620 | 0.824302 | 8.688488e-54 | -8.615850e-47 |
| Msa0140400 | Msa0650620 | 0.812850 | 3.445294e-51 | -8.615850e-47 |
| Msa0140510 | Msa0650620 | -0.808195 | 3.489854e-50 | -8.615850e-47 |
| Msa0140520 | Msa0650620 | -0.838753 | 2.384529e-57 | -8.615850e-47 |
| Msa0143080 | Msa0650620 | 0.824273 | 8.824535e-54 | -8.615850e-47 |
| Msa0145950 | Msa0650620 | 0.830017 | 3.720273e-55 | -8.615850e-47 |
| Msa0148120 | Msa0650620 | 0.819827 | 9.467468e-53 | -8.615850e-47 |
| Msa0152840 | Msa0650620 | 0.830330 | 3.121237e-55 | -8.615850e-47 |
| Msa0154770 | Msa0650620 | 0.816912 | 4.329141e-52 | -8.615850e-47 |
| Msa0168640 | Msa0650620 | -0.812386 | 4.351309e-51 | -8.615850e-47 |
| Msa0173330 | Msa0650620 | 0.846192 | 2.533865e-59 | -8.615850e-47 |
| Msa0178990 | Msa0650620 | 0.835815 | 1.347279e-56 | -8.615850e-47 |
| Msa0183460 | Msa0650620 | 0.817590 | 3.047000e-52 | -8.615850e-47 |
| Msa0194970 | Msa0650620 | 0.807961 | 3.914563e-50 | -8.615850e-47 |
| Msa0198940 | Msa0650620 | 0.801939 | 7.106089e-49 | -8.615850e-47 |
| Msa0208810 | Msa0650620 | 0.829985 | 3.789305e-55 | -8.615850e-47 |
| Msa0212750 | Msa0650620 | -0.879813 | 9.174131e-70 | -8.615850e-47 |
| Msa0219250 | Msa0650620 | -0.802487 | 5.481351e-49 | -8.615850e-47 |
| Msa0223100 | Msa0650620 | 0.839173 | 1.856771e-57 | -8.615850e-47 |
| Msa0223290 | Msa0650620 | 0.847695 | 9.826658e-60 | -8.615850e-47 |
| Msa0223380 | Msa0650620 | 0.818281 | 2.127926e-52 | -8.615850e-47 |
| Msa0227310 | Msa0650620 | -0.810758 | 9.829636e-51 | -8.615850e-47 |
| Msa0236980 | Msa0650620 | 0.834537 | 2.831111e-56 | -8.615850e-47 |
| Msa0370310 | Msa0650620 | 0.819997 | 8.653824e-53 | -8.615850e-47 |
| Msa0370870 | Msa0650620 | 0.801061 | 1.075335e-48 | -8.615850e-47 |
| Msa0374250 | Msa0650620 | 0.811647 | 6.305647e-51 | -8.615850e-47 |
| Msa0377530 | Msa0650620 | 0.829068 | 6.330567e-55 | -8.615850e-47 |
| Msa0394270 | Msa0650620 | 0.801234 | 9.914440e-49 | -8.615850e-47 |
| Msa0403190 | Msa0650620 | 0.832296 | 1.025166e-55 | -8.615850e-47 |
| Msa0404700 | Msa0650620 | 0.838137 | 3.438832e-57 | -8.615850e-47 |
| Msa0416320 | Msa0650620 | 0.826425 | 2.731558e-54 | -8.615850e-47 |
| Msa0419640 | Msa0650620 | -0.833404 | 5.437904e-56 | -8.615850e-47 |
| Msa0426810 | Msa0650620 | 0.855730 | 5.183593e-62 | -8.615850e-47 |
| Msa0429290 | Msa0650620 | 0.800967 | 1.124215e-48 | -8.615850e-47 |
| Msa0440130 | Msa0650620 | -0.811905 | 5.540578e-51 | -8.615850e-47 |
| Msa0449820 | Msa0650620 | 0.836544 | 8.794424e-57 | -8.615850e-47 |
| Msa0462540 | Msa0650620 | 0.833948 | 3.977069e-56 | -8.615850e-47 |
| Msa0464900 | Msa0650620 | 0.808908 | 2.458160e-50 | -8.615850e-47 |
| Msa0469790 | Msa0650620 | 0.829137 | 6.091736e-55 | -8.615850e-47 |
| Msa0469820 | Msa0650620 | 0.805506 | 1.291928e-49 | -8.615850e-47 |
| Msa0469910 | Msa0650620 | -0.818187 | 2.233827e-52 | -8.615850e-47 |
| Msa0471790 | Msa0650620 | 0.829089 | 6.256658e-55 | -8.615850e-47 |
| Msa0479090 | Msa0650620 | 0.804342 | 2.261879e-49 | -8.615850e-47 |
| Msa0239940 | Msa0650620 | 0.803069 | 4.156790e-49 | -8.615850e-47 |
| Msa0241740 | Msa0650620 | 0.826603 | 2.477844e-54 | -8.615850e-47 |
| Msa0266210 | Msa0650620 | 0.863765 | 1.970824e-64 | -8.615850e-47 |
| Msa0275400 | Msa0650620 | -0.839669 | 1.380252e-57 | -8.615850e-47 |
| Msa0279210 | Msa0650620 | -0.835789 | 1.367226e-56 | -8.615850e-47 |
| Msa0287670 | Msa0650620 | 0.864084 | 1.567546e-64 | -8.615850e-47 |
| Msa0288730 | Msa0650620 | 0.813250 | 2.814586e-51 | -8.615850e-47 |
| Msa0289400 | Msa0650620 | 0.804920 | 1.713317e-49 | -8.615850e-47 |
| Msa0295000 | Msa0650620 | -0.828339 | 9.500858e-55 | -8.615850e-47 |
| Msa0302650 | Msa0650620 | 0.856205 | 3.765169e-62 | -8.615850e-47 |
| Msa0308220 | Msa0650620 | 0.815132 | 1.081262e-51 | -8.615850e-47 |
| Msa0318970 | Msa0650620 | -0.821826 | 3.284428e-53 | -8.615850e-47 |
| Msa0326670 | Msa0650620 | 0.800476 | 1.416028e-48 | -8.615850e-47 |
| Msa0328210 | Msa0650620 | 0.835412 | 1.703669e-56 | -8.615850e-47 |
| Msa0347610 | Msa0650620 | 0.806008 | 1.013131e-49 | -8.615850e-47 |
| Msa0362060 | Msa0650620 | 0.858596 | 7.392313e-63 | -8.615850e-47 |
| Msa0363170 | Msa0650620 | 0.833544 | 5.017665e-56 | -8.615850e-47 |
| Msa0489610 | Msa0650620 | 0.828875 | 7.048814e-55 | -8.615850e-47 |
| Msa0505060 | Msa0650620 | 0.828791 | 7.388655e-55 | -8.615850e-47 |
| Msa0517490 | Msa0650620 | 0.811911 | 5.524145e-51 | -8.615850e-47 |
| Msa0524200 | Msa0650620 | -0.813223 | 2.854287e-51 | -8.615850e-47 |
| Msa0524600 | Msa0650620 | 0.806192 | 9.267575e-50 | -8.615850e-47 |
| Msa0530380 | Msa0650620 | 0.808138 | 3.587600e-50 | -8.615850e-47 |
| Msa0553220 | Msa0650620 | -0.808308 | 3.301026e-50 | -8.615850e-47 |
| Msa0554180 | Msa0650620 | -0.820442 | 6.845484e-53 | -8.615850e-47 |
| Msa0575990 | Msa0650620 | -0.818340 | 2.063257e-52 | -8.615850e-47 |
| Msa0576100 | Msa0650620 | -0.820580 | 6.362159e-53 | -8.615850e-47 |
| Msa0578090 | Msa0650620 | 0.806322 | 8.701207e-50 | -8.615850e-47 |
| Msa0579340 | Msa0650620 | 0.858431 | 8.280203e-63 | -8.615850e-47 |
| Msa0579350 | Msa0650620 | 0.849766 | 2.618516e-60 | -8.615850e-47 |
| Msa0579370 | Msa0650620 | 0.859542 | 3.847832e-63 | -8.615850e-47 |
| Msa0581920 | Msa0650620 | 0.833378 | 5.520752e-56 | -8.615850e-47 |
| Msa0586850 | Msa0650620 | 0.805471 | 1.314004e-49 | -8.615850e-47 |
| Msa0598560 | Msa0650620 | 0.802411 | 5.681746e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0650620 | MtrunA17_Chr4g0042961 | 91.200 | 125 | 8 | 1 | 1 | 122 | 1 | 125 | 5.57e-68 | 200 |
| Msa0650620 | MtrunA17_Chr4g0042951 | 66.667 | 132 | 31 | 4 | 3 | 122 | 11 | 141 | 1.75e-40 | 131 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0650620 | AT2G03440.1 | 42.636 | 129 | 52 | 5 | 11 | 122 | 64 | 187 | 4.74e-14 | 65.5 |
| Msa0650620 | AT1G13930.3 | 48.062 | 129 | 47 | 8 | 8 | 122 | 33 | 155 | 2.28e-13 | 63.2 |
| Msa0650620 | AT1G13930.1 | 48.062 | 129 | 47 | 8 | 8 | 122 | 33 | 155 | 2.28e-13 | 63.2 |
| Msa0650620 | AT1G13930.2 | 48.062 | 129 | 47 | 8 | 8 | 122 | 33 | 155 | 2.28e-13 | 63.2 |
Find 25 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTTCAACCAGTGAGCTTA+TGG | 0.299153 | 4_3:+57813623 | Msa0650620:CDS |
| AGGCAGCACAGTCAGGTTTC+GGG | 0.304942 | 4_3:+57813667 | Msa0650620:CDS |
| GAGGCAGCACAGTCAGGTTT+CGG | 0.309235 | 4_3:+57813666 | Msa0650620:CDS |
| CCACCTGATCCTCCACCTTC+AGG | 0.341637 | 4_3:-57813897 | None:intergenic |
| AGTTGGTCAGTATGCTAAAT+TGG | 0.398975 | 4_3:+57813743 | Msa0650620:CDS |
| AAGCCTGAAGGTGGAGGATC+AGG | 0.423090 | 4_3:+57813894 | Msa0650620:CDS |
| TGCACTTGCCATAAGCTCAC+TGG | 0.466727 | 4_3:-57813631 | None:intergenic |
| AGCTGCTGATTATCTGCATA+AGG | 0.469713 | 4_3:+57813800 | Msa0650620:CDS |
| GGTGGAGGATCAGGTGGACT+AGG | 0.476662 | 4_3:+57813903 | Msa0650620:CDS |
| GACAAGACAGCAGAAGCTGC+AGG | 0.506059 | 4_3:+57813705 | Msa0650620:CDS |
| GTAGCAGAGGCAGCACAGTC+AGG | 0.527765 | 4_3:+57813660 | Msa0650620:CDS |
| TGATCTGCAGGTTTAGAAGC+TGG | 0.532370 | 4_3:-57813849 | None:intergenic |
| AAGGTTGAGAACACTACTAC+TGG | 0.547092 | 4_3:+57813819 | Msa0650620:CDS |
| AAGTGCAAAGGTGGTAGCAG+AGG | 0.549500 | 4_3:+57813647 | Msa0650620:CDS |
| TTGGATGAACAGAAAGGTGT+AGG | 0.558648 | 4_3:+57813762 | Msa0650620:CDS |
| GCTAAATTGGATGAACAGAA+AGG | 0.567227 | 4_3:+57813756 | Msa0650620:CDS |
| CCTGAAGGTGGAGGATCAGG+TGG | 0.601078 | 4_3:+57813897 | Msa0650620:CDS |
| TGAGCTTATGGCAAGTGCAA+AGG | 0.606848 | 4_3:+57813635 | Msa0650620:CDS |
| GGAGGATCAGGTGGACTAGG+TGG | 0.611533 | 4_3:+57813906 | Msa0650620:CDS |
| GCTTATGGCAAGTGCAAAGG+TGG | 0.622253 | 4_3:+57813638 | Msa0650620:CDS |
| GGTGATCTTCTAGATGCAGT+TGG | 0.625884 | 4_3:+57813726 | Msa0650620:CDS |
| GTTGCTAAGCCTGAAGGTGG+AGG | 0.637775 | 4_3:+57813888 | Msa0650620:CDS |
| TCTGCAGGTTTAGAAGCTGG+TGG | 0.662750 | 4_3:-57813846 | None:intergenic |
| GATGATGTTGCTAAGCCTGA+AGG | 0.667542 | 4_3:+57813882 | Msa0650620:CDS |
| GATGTTGCTAAGCCTGAAGG+TGG | 0.674394 | 4_3:+57813885 | Msa0650620:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAGCAACATCATCACTTTT+AGG | - | chr4_3:57813876-57813895 | None:intergenic | 30.0% |
| AGTTGGTCAGTATGCTAAAT+TGG | + | chr4_3:57813743-57813762 | Msa0650620:CDS | 35.0% | |
| GCTAAATTGGATGAACAGAA+AGG | + | chr4_3:57813756-57813775 | Msa0650620:CDS | 35.0% | |
| GATTTCAACCAGTGAGCTTA+TGG | + | chr4_3:57813623-57813642 | Msa0650620:CDS | 40.0% | |
| ! | TTGGATGAACAGAAAGGTGT+AGG | + | chr4_3:57813762-57813781 | Msa0650620:CDS | 40.0% |
| ! | AGCTGCTGATTATCTGCATA+AGG | + | chr4_3:57813800-57813819 | Msa0650620:CDS | 40.0% |
| AAGGTTGAGAACACTACTAC+TGG | + | chr4_3:57813819-57813838 | Msa0650620:CDS | 40.0% | |
| ! | ACTAGGTGGTGATTTTGCTA+AGG | + | chr4_3:57813920-57813939 | Msa0650620:CDS | 40.0% |
| TGAGCTTATGGCAAGTGCAA+AGG | + | chr4_3:57813635-57813654 | Msa0650620:CDS | 45.0% | |
| GGTGATCTTCTAGATGCAGT+TGG | + | chr4_3:57813726-57813745 | Msa0650620:CDS | 45.0% | |
| TGATCTGCAGGTTTAGAAGC+TGG | - | chr4_3:57813852-57813871 | None:intergenic | 45.0% | |
| !! | TCACTTTTAGGCTGATCTGC+AGG | - | chr4_3:57813864-57813883 | None:intergenic | 45.0% |
| GATGATGTTGCTAAGCCTGA+AGG | + | chr4_3:57813882-57813901 | Msa0650620:CDS | 45.0% | |
| ! | GGTGATTTTGCTAAGGTTGC+TGG | + | chr4_3:57813927-57813946 | Msa0650620:CDS | 45.0% |
| ! | GATTTTGCTAAGGTTGCTGG+AGG | + | chr4_3:57813930-57813949 | Msa0650620:CDS | 45.0% |
| TGCACTTGCCATAAGCTCAC+TGG | - | chr4_3:57813634-57813653 | None:intergenic | 50.0% | |
| GCTTATGGCAAGTGCAAAGG+TGG | + | chr4_3:57813638-57813657 | Msa0650620:CDS | 50.0% | |
| AAGTGCAAAGGTGGTAGCAG+AGG | + | chr4_3:57813647-57813666 | Msa0650620:CDS | 50.0% | |
| TCTGCAGGTTTAGAAGCTGG+TGG | - | chr4_3:57813849-57813868 | None:intergenic | 50.0% | |
| GATGTTGCTAAGCCTGAAGG+TGG | + | chr4_3:57813885-57813904 | Msa0650620:CDS | 50.0% | |
| GAGGCAGCACAGTCAGGTTT+CGG | + | chr4_3:57813666-57813685 | Msa0650620:CDS | 55.0% | |
| AGGCAGCACAGTCAGGTTTC+GGG | + | chr4_3:57813667-57813686 | Msa0650620:CDS | 55.0% | |
| GACAAGACAGCAGAAGCTGC+AGG | + | chr4_3:57813705-57813724 | Msa0650620:CDS | 55.0% | |
| GTTGCTAAGCCTGAAGGTGG+AGG | + | chr4_3:57813888-57813907 | Msa0650620:CDS | 55.0% | |
| AAGCCTGAAGGTGGAGGATC+AGG | + | chr4_3:57813894-57813913 | Msa0650620:CDS | 55.0% | |
| GTAGCAGAGGCAGCACAGTC+AGG | + | chr4_3:57813660-57813679 | Msa0650620:CDS | 60.0% | |
| CCACCTGATCCTCCACCTTC+AGG | - | chr4_3:57813900-57813919 | None:intergenic | 60.0% | |
| CCTGAAGGTGGAGGATCAGG+TGG | + | chr4_3:57813897-57813916 | Msa0650620:CDS | 60.0% | |
| ! | GGTGGAGGATCAGGTGGACT+AGG | + | chr4_3:57813903-57813922 | Msa0650620:CDS | 60.0% |
| !! | GGAGGATCAGGTGGACTAGG+TGG | + | chr4_3:57813906-57813925 | Msa0650620:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_3 | gene | 57813597 | 57813965 | 57813597 | ID=Msa0650620;Name=Msa0650620 |
| chr4_3 | mRNA | 57813597 | 57813965 | 57813597 | ID=Msa0650620-mRNA-1;Parent=Msa0650620;Name=Msa0650620-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0|-1|0|1|-1|1|1|0|122 |
| chr4_3 | exon | 57813597 | 57813965 | 57813597 | ID=Msa0650620-mRNA-1:exon:15160;Parent=Msa0650620-mRNA-1 |
| chr4_3 | CDS | 57813597 | 57813965 | 57813597 | ID=Msa0650620-mRNA-1:cds;Parent=Msa0650620-mRNA-1 |
| Gene Sequence |
| Protein sequence |
