AlfalfaGEDB | Msa0756550

M. sativa cultivar zhongmu-4 / Msa0756550

KEGG: K00512|K00517|K07418|K13260|K20623
GO: GO:0001666 GO:0002213 GO:0002376 GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006082 GO:0006725 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009682 GO:0009759 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016143 GO:0016144 GO:0016491 GO:0016705 GO:0016709 GO:0018130 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0036293 GO:0036294 GO:0042221 GO:0042343 GO:0042430 GO:0042435 GO:0042545 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046483 GO:0050832 GO:0050896 GO:0051179 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0055114 GO:0070482 GO:0070727 GO:0070887 GO:0071453 GO:0071456 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659
PFAM: PF00067.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0756550	sp\|Q6WNQ8\|C81E8_MEDTR	90.381	499	22	2	1	473	1	499	0.0	927

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0756550	G7K579	91.182	499	18	2	1	473	1	499	0.0	934

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0004840	Msa0756550	0.819544	1.098358e-52	-8.615850e-47
Msa0051750	Msa0756550	0.801475	8.850071e-49	-8.615850e-47
Msa0065950	Msa0756550	0.834634	2.675571e-56	-8.615850e-47
Msa0096980	Msa0756550	0.818149	2.278886e-52	-8.615850e-47
Msa0145010	Msa0756550	0.819391	1.190105e-52	-8.615850e-47
Msa0190120	Msa0756550	0.816976	4.188815e-52	-8.615850e-47
Msa0206010	Msa0756550	0.806735	7.121782e-50	-8.615850e-47
Msa0220440	Msa0756550	0.804467	2.130038e-49	-8.615850e-47
Msa0271890	Msa0756550	0.802898	4.509593e-49	-8.615850e-47
Msa0284820	Msa0756550	0.815714	8.023530e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0756550	MtrunA17_Chr5g0401681	91.182	499	18	2	1	473	1	499	0.0	934
Msa0756550	MtrunA17_Chr4g0053621	63.563	494	150	3	1	464	1	494	0.0	644
Msa0756550	MtrunA17_Chr4g0052861	58.740	492	171	4	2	468	10	494	0.0	615
Msa0756550	MtrunA17_Chr4g0052881	60.246	488	165	3	6	464	4	491	0.0	598
Msa0756550	MtrunA17_Chr2g0296661	64.228	492	146	3	2	463	3	494	0.0	593
Msa0756550	MtrunA17_Chr5g0401691	57.787	488	182	5	4	468	25	511	0.0	577
Msa0756550	MtrunA17_Chr4g0052851	55.626	471	192	4	14	468	17	486	0.0	548
Msa0756550	MtrunA17_Chr4g0017411	56.197	468	186	6	14	464	14	479	0.0	536
Msa0756550	MtrunA17_Chr2g0296651	54.970	493	194	3	4	468	2	494	0.0	532
Msa0756550	MtrunA17_Chr4g0039391	44.956	456	220	5	41	465	45	500	5.39e-140	412

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0756550	AT4G37330.1	55.509	481	188	7	6	464	9	485	0.0	536
Msa0756550	AT4G37370.1	53.306	484	195	6	8	464	7	486	0.0	535
Msa0756550	AT5G36220.1	54.303	488	188	7	7	464	12	494	0.0	534
Msa0756550	AT4G37340.1	55.694	483	180	8	13	464	10	489	0.0	534
Msa0756550	AT4G37320.1	51.760	483	205	4	8	464	8	488	0.0	520
Msa0756550	AT4G37360.1	53.673	490	189	8	3	464	9	488	4.79e-180	513
Msa0756550	AT5G57220.1	51.446	484	209	6	4	464	3	483	2.56e-178	509
Msa0756550	AT3G28740.1	56.957	460	173	6	30	464	38	497	1.44e-177	508
Msa0756550	AT2G23190.1	50.305	491	214	4	1	464	45	532	9.80e-175	502
Msa0756550	AT4G37400.1	50.713	491	208	7	4	464	3	489	2.62e-172	494

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Find 87 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACATCCTTAATATTCAA+TGG	0.453871	5_2:-10876177	None:intergenic
AAATCTTCTGAAGACAAATG+AGG	0.612691	5_2:-10875817	None:intergenic
AAATTACTTTCATTTGGGTT+GGG	0.453431	5_2:+10875976	Msa0756550:CDS
AACCGACCTCATTTCTTAAC+TGG	0.318085	5_2:+10873220	Msa0756550:CDS
AAGGACTGTTGGATTGACTT+TGG	0.404254	5_2:+10876032	Msa0756550:CDS
AAGTCAATCCAACAGTCCTT+TGG	0.402768	5_2:-10876029	None:intergenic
AATCACCTTATGGTGACCAT+TGG	0.528124	5_2:+10873278	Msa0756550:CDS
AATCATCAAAGGACTTATGG+TGG	0.639977	5_2:+10873797	Msa0756550:CDS
AATGAGTTGGACACTCACAT+AGG	0.637656	5_2:+10875691	Msa0756550:CDS
AATGTCATCTCCGAAAACAT+AGG	0.567870	5_2:-10873448	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAATTATAGGAAGACATTGT+GGG	-	chr5_2:10873036-10873055	None:intergenic	30.0%
CACACCTTATCACAAAAATA+TGG	+	chr5_2:10873097-10873116	Msa0756550:CDS	30.0%
TCATCGTTTAAATTCCTTCT+TGG	+	chr5_2:10873342-10873361	Msa0756550:CDS	30.0%
TACTATAATGAGAATGGTGT+CGG	+	chr5_2:10873474-10873493	Msa0756550:CDS	30.0%
TGTTAAGAGATGATCAATCA+TGG	-	chr5_2:10873742-10873761	None:intergenic	30.0%
ATCACAACCTGAGTATTATA+CGG	+	chr5_2:10873770-10873789	Msa0756550:CDS	30.0%
ATTTGATCCGTATAATACTC+AGG	-	chr5_2:10873780-10873799	None:intergenic	30.0%
TATACGGATCAAATCATCAA+AGG	+	chr5_2:10873786-10873805	Msa0756550:CDS	30.0%
TCAAATCATCAAAGGACTTA+TGG	+	chr5_2:10873794-10873813	Msa0756550:CDS	30.0%
GTTACTGAATGGATAAGTTT+CGG	+	chr5_2:10874055-10874074	Msa0756550:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	10872932	10876374	10872932	ID=Msa0756550;Name=Msa0756550
chr5_2	mRNA	10872932	10876374	10872932	ID=Msa0756550-mRNA-1;Parent=Msa0756550;Name=Msa0756550-mRNA-1;_AED=0.27;_eAED=0.27;_QI=0\|0.5\|0.33\|1\|1\|1\|3\|171\|473
chr5_2	exon	10872932	10873551	10872932	ID=Msa0756550-mRNA-1:exon:4896;Parent=Msa0756550-mRNA-1
chr5_2	exon	10873626	10873818	10873626	ID=Msa0756550-mRNA-1:exon:4897;Parent=Msa0756550-mRNA-1
chr5_2	exon	10875595	10876374	10875595	ID=Msa0756550-mRNA-1:exon:4898;Parent=Msa0756550-mRNA-1
chr5_2	CDS	10872932	10873551	10872932	ID=Msa0756550-mRNA-1:cds;Parent=Msa0756550-mRNA-1
chr5_2	CDS	10873626	10873818	10873626	ID=Msa0756550-mRNA-1:cds;Parent=Msa0756550-mRNA-1
chr5_2	CDS	10875595	10876203	10875595	ID=Msa0756550-mRNA-1:cds;Parent=Msa0756550-mRNA-1
chr5_2	three_prime_UTR	10876204	10876374	10876204	ID=Msa0756550-mRNA-1:three_prime_utr;Parent=Msa0756550-mRNA-1

Gene Sequence

>Msa0756550

ATGAGTACTTTCTATCTCTCACTCATCTCTCTCTTTTTCCTTATCATCACTTTGAAGCTCTTCTTCAACACATCAAGAAAGATCAAAAACCTTCCACCAGGCCCACAATGTCTTCCTATAATTGGAAACCTTCACCAACTCAAACAACCACTCCACCACACATTCCACACCTTATCACAAAAATATGGCCAAATCTTCTCTCTTTGGTTCGGTTCTCGTCTCGTTGTTGTTGTTTCATCATTAACCATGACACAAGAATGTTTTACCAAAAACGACATAGTTTTAGCAAACCGACCTCATTTCTTAACTGGAAAATATATTGGCTACAACAATACTACGGTAGCACAATCACCTTATGGTGACCATTGGCGCAATCTTCGACGTATATTATCAATTGAAGTACTCTCATCTCATCGTTTAAATTCCTTCTTGGAAATACGAAGAGATGAGATTATGAGACTAATACAAAAGTTAGCACAAAAATCATACAATGGTTTCACTGAAGTTGAACTTAGACCTATGTTTTCGGAGATGACATTTAATACTATAATGAGAATGGTGTCGGGGAAAAGATACTATGGAAATGATTGTGATGTGAGTGATGTTGAGGAAGCAAGATTGTGGTTTGATTTTGATGGTTTGGAAAAAAGGCTTAAGAAGATTAGTAAGAGAACCGATGCTTTTTTACAAGGGCTTATTGATGAACATCGTTTTGGAAAGAGGAATAGTAACACCATGATTGATCATCTCTTAACACAGCAACAATCACAACCTGAGTATTATACGGATCAAATCATCAAAGGACTTATGGTGGTTATGTTACTTGCGGGAACAGACACATCATCCGTGACATTAGAATGGGCTATGTCCAGTTTACTAAACCATCCAGAAATAATGAAGATGGCAAAAAATGAGTTGGACACTCACATAGGACAAGAACGTTTAGTAGATGAGCCTGACATTTCAAAACTTCCTTATCTTCAAAGCATTGTCTATGAAACCCTTCGACTACATGCAGCAGCTCCATTATTAGTACCTCATTTGTCTTCAGAAGATTTTACCTTAGGAGGATATAATATCCCACAAAACACAATTTTGATGGTGAATGCTTGGGTCATTCATAGAGATCCAAATTTATGGAGTGATCCTACATGTTTTAAGCCAGAGAGGTTTGAGAAAGAAGGTGAGGTAAATAAATTACTTTCATTTGGGTTGGGTAGAAGGGCTTGTCCTGGAGAAAGCTTAGCCCAAAGGACTGTTGGATTGACTTTGGGTTTGTTGATTCAGTGTTTTGAGTGGAAACGAATAGGTGAAGAAAAAATTGACATGGTTGAAGCAAAAGGAATCACGGTGGGAAAAAAGATTTCCTTAAAAGCAATGTGTAAAGTGCGACATCCATTGAATATTAAGGATGTTTTTTAG

Protein sequence

>Msa0756550

MSTFYLSLISLFFLIITLKLFFNTSRKIKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQIFSLWFGSRLVVVVSSLTMTQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNLRRILSIEVLSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTIMRMVSGKRYYGNDCDVSDVEEARLWFDFDGLEKRLKKISKRTDAFLQGLIDEHRFGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVMLLAGTDTSSVTLEWAMSSLLNHPEIMKMAKNELDTHIGQERLVDEPDISKLPYLQSIVYETLRLHAAAPLLVPHLSSEDFTLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEKEGEVNKLLSFGLGRRACPGESLAQRTVGLTLGLLIQCFEWKRIGEEKIDMVEAKGITVGKKISLKAMCKVRHPLNIKDVF*