AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0220440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0220440 MtrunA17_Chr2g0324061 93.737 479 21 1 1 479 1 470 0.0 881
Msa0220440 MtrunA17_Chr4g0022171 79.835 486 75 6 1 479 1 470 0.0 762
Msa0220440 MtrunA17_Chr7g0249421 50.110 453 191 6 45 476 50 488 1.70e-145 426
Msa0220440 MtrunA17_Chr7g0221691 51.562 384 159 8 1 373 42 409 1.40e-129 383
Msa0220440 MtrunA17_Chr7g0243571 44.391 419 189 7 56 473 57 432 1.16e-114 345
Msa0220440 MtrunA17_Chr8g0342431 46.961 362 129 7 45 372 40 372 1.38e-103 315
Msa0220440 MtrunA17_Chr7g0272061 45.732 328 163 4 55 380 55 369 7.44e-102 310
Msa0220440 MtrunA17_Chr4g0067711 46.945 311 143 5 63 372 63 352 2.54e-101 308
Msa0220440 MtrunA17_Chr2g0295401 48.243 313 140 5 61 372 62 353 2.86e-101 307
Msa0220440 MtrunA17_Chr4g0004971 46.341 328 150 6 51 373 38 344 3.52e-100 305
Msa0220440 MtrunA17_Chr1g0192421 45.141 319 153 4 55 372 53 350 1.64e-99 303
Msa0220440 MtrunA17_Chr1g0151781 47.352 321 147 4 55 375 33 331 2.42e-88 273
Msa0220440 MtrunA17_Chr4g0066371 27.414 321 168 10 73 374 68 342 3.20e-25 107
Msa0220440 MtrunA17_Chr6g0486661 29.938 324 159 12 73 374 106 383 4.73e-25 109
Msa0220440 MtrunA17_Chr5g0396731 31.457 302 141 10 44 334 54 300 4.98e-24 103
Msa0220440 MtrunA17_Chr2g0286951 28.267 329 171 11 73 382 69 351 2.32e-22 99.0
Msa0220440 MtrunA17_Chr3g0135961 27.419 310 161 12 25 331 236 484 1.45e-18 88.6
Msa0220440 MtrunA17_Chr5g0435721 35.829 187 82 7 159 332 258 419 3.03e-18 87.4
Msa0220440 MtrunA17_Chr6g0483411 29.565 230 114 8 160 371 119 318 2.06e-17 83.2
Msa0220440 MtrunA17_Chr3g0136711 30.252 238 130 8 147 381 87 291 2.67e-17 82.4
Msa0220440 MtrunA17_Chr1g0150451 31.863 204 112 6 147 349 130 307 1.33e-16 80.9
Msa0220440 MtrunA17_Chr4g0070871 27.869 244 127 8 88 331 251 445 5.96e-16 80.1
Msa0220440 MtrunA17_Chr1g0212961 29.434 265 113 11 90 349 126 321 1.18e-15 78.2
Msa0220440 MtrunA17_Chr1g0150781 26.101 318 166 12 17 331 237 488 2.37e-14 75.5
Msa0220440 MtrunA17_Chr2g0279151 31.068 206 115 8 147 351 87 266 2.58e-14 73.2
Msa0220440 MtrunA17_Chr4g0050381 30.583 206 110 10 148 349 84 260 6.75e-14 72.0
Msa0220440 MtrunA17_Chr2g0299981 27.519 258 152 8 76 327 248 476 7.25e-14 73.9
Msa0220440 MtrunA17_Chr1g0175381 26.524 328 174 16 87 385 76 365 1.05e-13 72.8
Msa0220440 MtrunA17_Chr1g0194191 28.676 272 130 13 90 351 118 335 1.49e-13 72.4
Msa0220440 MtrunA17_Chr2g0317021 34.921 189 88 9 186 372 177 332 1.94e-13 71.2
Msa0220440 MtrunA17_Chr3g0115851 33.019 212 101 11 165 371 106 281 2.78e-13 70.1
Msa0220440 MtrunA17_Chr8g0360351 33.163 196 101 9 158 349 96 265 3.13e-13 70.1
Msa0220440 MtrunA17_Chr3g0136811 27.458 295 152 12 65 332 28 287 1.35e-12 68.9
Msa0220440 MtrunA17_Chr1g0158781 31.034 174 93 5 159 332 184 330 2.78e-12 68.2
Msa0220440 MtrunA17_Chr4g0051661 32.984 191 98 8 161 349 219 381 8.09e-12 67.0
Msa0220440 MtrunA17_Chr3g0126511 27.413 259 126 8 92 349 127 324 1.08e-11 66.2
Msa0220440 MtrunA17_Chr5g0407161 26.178 191 104 6 149 332 148 308 3.45e-11 64.7
Msa0220440 MtrunA17_Chr3g0144111 29.064 203 95 9 162 351 47 213 3.89e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0220440 AT4G03415.1 72.707 458 103 6 21 476 28 465 0.0 625
Msa0220440 AT4G03415.3 72.707 458 103 6 21 476 28 465 0.0 625
Msa0220440 AT4G03415.2 72.707 458 103 6 21 476 28 465 0.0 625
Msa0220440 AT1G03590.1 65.975 482 141 5 1 479 1 462 0.0 620
Msa0220440 AT1G16220.1 48.678 454 188 10 42 472 46 477 1.13e-143 421
Msa0220440 AT3G02750.2 46.918 503 221 9 1 472 1 488 1.45e-140 413
Msa0220440 AT3G02750.1 46.918 503 221 9 1 472 1 488 1.45e-140 413
Msa0220440 AT1G79630.8 47.894 451 188 8 51 472 54 486 2.55e-140 412
Msa0220440 AT1G79630.1 47.894 451 188 8 51 472 58 490 2.61e-140 413
Msa0220440 AT5G36250.2 47.333 450 171 8 45 472 52 457 2.34e-137 404
Msa0220440 AT5G36250.1 46.966 445 166 8 45 472 52 443 1.53e-134 396
Msa0220440 AT3G02750.3 43.866 538 221 10 1 472 1 523 2.16e-132 393
Msa0220440 AT1G79630.2 47.031 421 176 8 81 472 18 420 6.59e-125 371
Msa0220440 AT1G79630.7 47.031 421 176 8 81 472 18 420 6.59e-125 371
Msa0220440 AT1G79630.6 45.729 398 170 7 103 472 1 380 9.95e-111 333
Msa0220440 AT1G79630.3 45.729 398 170 7 103 472 1 380 9.95e-111 333
Msa0220440 AT1G79630.4 45.729 398 170 7 103 472 1 380 9.95e-111 333
Msa0220440 AT1G79630.5 45.729 398 170 7 103 472 1 380 9.95e-111 333
Msa0220440 AT5G36250.3 53.873 284 117 3 45 314 52 335 2.05e-106 320
Msa0220440 AT3G05640.2 45.963 322 147 4 52 372 54 349 5.59e-101 307
Msa0220440 AT3G05640.1 45.963 322 147 4 52 372 54 349 5.59e-101 307
Msa0220440 AT3G05640.3 45.963 322 147 4 52 372 54 349 5.59e-101 307
Msa0220440 AT5G01700.2 48.466 326 142 5 52 375 39 340 1.72e-100 306
Msa0220440 AT5G01700.1 50.000 312 130 5 66 375 4 291 4.57e-100 304
Msa0220440 AT5G27930.1 46.106 321 146 4 55 371 56 353 6.59e-100 305
Msa0220440 AT5G27930.2 46.106 321 146 4 55 371 56 353 6.59e-100 305
Msa0220440 AT3G16800.2 40.867 323 161 4 52 372 54 348 7.19e-82 258
Msa0220440 AT3G16800.1 40.867 323 161 4 52 372 54 348 7.19e-82 258
Msa0220440 AT3G16800.3 40.867 323 161 4 52 372 54 348 7.19e-82 258
Msa0220440 AT5G27930.3 45.038 262 126 3 56 313 57 304 1.49e-81 256
Msa0220440 AT4G32950.1 39.791 382 168 9 1 379 1 323 2.03e-77 245
Msa0220440 AT5G26010.1 43.082 318 148 6 59 375 42 327 1.70e-76 243
Msa0220440 AT3G16800.4 40.214 281 139 3 93 372 1 253 2.02e-69 222
Msa0220440 AT3G16800.6 40.214 281 139 3 93 372 1 253 2.02e-69 222
Msa0220440 AT4G32950.2 40.127 314 135 8 1 311 1 264 1.94e-60 199
Msa0220440 AT3G16800.5 35.519 183 97 3 54 234 56 219 3.20e-32 123
Msa0220440 AT2G20050.2 30.000 340 167 12 73 392 125 413 2.21e-28 119
Msa0220440 AT2G20050.1 30.000 340 167 12 73 392 125 413 2.22e-28 119
Msa0220440 AT3G11410.1 35.094 265 126 11 68 326 112 336 1.25e-20 94.4
Msa0220440 AT3G06270.1 26.923 260 136 8 73 313 70 294 2.53e-19 89.7
Msa0220440 AT1G07430.1 29.154 319 166 13 34 340 92 362 5.10e-18 86.7
Msa0220440 AT4G28400.3 34.804 204 106 9 147 349 67 244 6.73e-18 84.0
Msa0220440 AT5G10740.1 33.173 208 108 6 147 351 86 265 6.91e-18 85.5
Msa0220440 AT5G10740.2 33.173 208 108 6 147 351 86 265 6.91e-18 85.5
Msa0220440 AT4G28400.1 34.804 204 106 9 147 349 89 266 9.25e-18 84.0
Msa0220440 AT5G24940.1 32.524 206 112 6 147 351 86 265 1.28e-17 85.5
Msa0220440 AT4G31750.2 31.628 215 111 8 162 373 101 282 1.42e-16 80.9
Msa0220440 AT4G31750.1 31.455 213 114 7 162 373 101 282 1.79e-16 80.5
Msa0220440 AT4G31750.3 31.455 213 114 7 162 373 101 282 1.79e-16 80.5
Msa0220440 AT2G29380.1 29.930 284 120 14 68 331 85 309 6.56e-16 79.7
Msa0220440 AT1G43900.1 30.502 259 117 9 90 347 154 350 1.65e-15 78.6
Msa0220440 AT1G78200.5 33.161 193 105 6 160 351 54 223 9.19e-15 74.3
Msa0220440 AT1G78200.4 33.161 193 105 6 160 351 54 223 9.19e-15 74.3
Msa0220440 AT1G17550.1 30.126 239 133 7 90 327 237 442 2.63e-14 75.5
Msa0220440 AT1G17550.2 30.126 239 133 7 90 327 237 442 2.63e-14 75.5
Msa0220440 AT2G20630.1 32.843 204 110 9 147 349 85 262 8.36e-14 72.0
Msa0220440 AT2G20630.2 32.843 204 110 9 147 349 85 262 1.02e-13 72.0
Msa0220440 AT5G53140.1 29.412 204 117 5 147 349 154 331 1.97e-13 72.4
Msa0220440 AT5G53140.2 29.412 204 117 5 147 349 154 331 1.97e-13 72.4
Msa0220440 AT3G15260.2 32.995 197 102 9 157 349 102 272 3.99e-12 67.4
Msa0220440 AT3G15260.1 32.995 197 102 9 157 349 102 272 3.99e-12 67.4
Msa0220440 AT1G18030.1 26.522 230 121 8 160 371 149 348 5.00e-12 67.8
Msa0220440 AT4G28400.2 34.104 173 94 9 147 317 89 243 6.49e-12 66.2
Msa0220440 AT2G34740.3 35.429 175 88 10 140 310 131 284 2.06e-11 65.5
Msa0220440 AT2G34740.2 35.429 175 88 10 140 310 149 302 2.21e-11 65.5

Find 138 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCCTTGAAACTAGCTTTAT+TGG 0.084396 2_1:+68792746 None:intergenic
TCTTTCTGGAACTGAGAAAA+TGG 0.194323 2_1:-68793275 Msa0220440:exon
GCTGAAGTCTCATGCAAATT+TGG 0.195372 2_1:-68792616 Msa0220440:CDS
TTCTATAGTGAGAGGATCTT+TGG 0.223060 2_1:-68793408 Msa0220440:five_prime_UTR
TAAATATCCAACTTCGAAAA+TGG 0.246853 2_1:-68791562 Msa0220440:CDS
ACGGCCAAACTCTTGAAAAT+TGG 0.259748 2_1:-68791202 Msa0220440:exon
TTAAGCCATGAAAATTTCTT+AGG 0.262782 2_1:-68794018 Msa0220440:five_prime_UTR
GATGAGGTCAATGTGAATTC+TGG 0.264414 2_1:-68793719 Msa0220440:five_prime_UTR
AATTTAATATTTAATATATA+TGG 0.273123 2_1:+68793559 None:intergenic
TCGGATACCTCTTTGATTCT+CGG 0.283518 2_1:-68794163 Msa0220440:five_prime_UTR
TGCCTGAATGCCTGTTGCTT+TGG 0.290415 2_1:+68791448 None:intergenic
CTTCTAGACCTAACCAGTTT+TGG 0.316902 2_1:+68791274 None:intergenic
TATGACACACTTGGCAAATC+AGG 0.348941 2_1:+68792200 None:intergenic
ACCTAGTTGCACGCAAAGTT+AGG 0.349058 2_1:-68792830 Msa0220440:CDS
CTTTCTGGAACTGAGAAAAT+GGG 0.350287 2_1:-68793274 Msa0220440:exon
TGACCATCATCTGACTCAAC+TGG 0.351058 2_1:+68791423 None:intergenic
GTGAGAGGATCTTTGGAATC+AGG 0.358603 2_1:-68793401 Msa0220440:five_prime_UTR
GATCTACCAATGTTATTTGT+CGG 0.369663 2_1:-68794182 Msa0220440:five_prime_UTR
AATTTGTTCATGGGCAATAT+CGG 0.379510 2_1:-68792299 Msa0220440:CDS
TCTAGATGTTCATTTAATCT+TGG 0.380127 2_1:-68793854 Msa0220440:five_prime_UTR
TTAGCAATGGCTAGAGCATT+TGG 0.381409 2_1:-68791989 Msa0220440:CDS
ACTCCATCTTGTCTTGAAAT+CGG 0.382346 2_1:-68793208 Msa0220440:CDS
TCAGGGTTCAAATTTGTTCA+TGG 0.382381 2_1:-68792309 Msa0220440:intron
ATCAGGATGCCATGATTGTA+TGG 0.384111 2_1:-68793039 Msa0220440:CDS
CCATTACTAAATATTCTGTT+TGG 0.386029 2_1:+68793112 None:intergenic
TTCTTGTATATTTACGCAGC+AGG 0.386376 2_1:-68793077 Msa0220440:CDS
TACACATGTCTCATTATATT+CGG 0.390786 2_1:+68791356 None:intergenic
TTATCCATTCATATAGCTTA+CGG 0.391785 2_1:-68793506 Msa0220440:five_prime_UTR
CACACAAATTTAGATATTTG+TGG 0.393174 2_1:+68793828 None:intergenic
ATCTGTGACGGTAGTGAAAC+AGG 0.395381 2_1:-68792571 Msa0220440:intron
TTGAAAATTGGTTATTCAGT+TGG 0.395918 2_1:-68791190 Msa0220440:exon
ACATGTGTAGGAGGAGGAAA+AGG 0.396810 2_1:-68791342 Msa0220440:CDS
AACCACACCCTACAAACTTC+TGG 0.399419 2_1:+68792034 None:intergenic
GGGTCGTAAGGGTGTTAATC+AGG 0.402268 2_1:-68793056 Msa0220440:CDS
TTTACTCAATTGACCAAGCT+TGG 0.403894 2_1:-68793374 Msa0220440:five_prime_UTR
CATCAAGTCATTGCTGTCCT+TGG 0.412532 2_1:+68792253 None:intergenic
CTATTAAATTAAATCAAATC+CGG 0.415351 2_1:+68793760 None:intergenic
CAAGGACAGCAATGACTTGA+TGG 0.418227 2_1:-68792252 Msa0220440:CDS
TGAATGCCTGTTGCTTTGGA+TGG 0.421566 2_1:+68791452 None:intergenic
ATCATCGGCAGCGAGGGTTC+TGG 0.421788 2_1:-68791613 Msa0220440:CDS
CGGTGACTCGAGAGCAATCA+TGG 0.423084 2_1:-68792279 Msa0220440:CDS
ATCCAAAGCAACAGGCATTC+AGG 0.423868 2_1:-68791450 Msa0220440:CDS
AGAAGCCGGTGCGTAAATTC+AGG 0.424088 2_1:+68791929 None:intergenic
CATCAGCTGAAGACCAAAAC+TGG 0.424537 2_1:-68791287 Msa0220440:CDS
AGGATGAACCAGAAGTTTGT+AGG 0.433167 2_1:-68792042 Msa0220440:CDS
ACTAAATATTCTGTTTGGTA+AGG 0.434667 2_1:+68793117 None:intergenic
TTCATTGTTCTTGCCTCGGA+TGG 0.438125 2_1:-68791890 Msa0220440:CDS
ATGAGGTCAATGTGAATTCT+GGG 0.447942 2_1:-68793718 Msa0220440:five_prime_UTR
TTGTAATGCTTATAACAATA+CGG 0.448522 2_1:-68791158 Msa0220440:three_prime_UTR
GTGAAGTTCCTTCGCAAAGA+TGG 0.450703 2_1:+68791390 None:intergenic
AATTCACACCTTGTTGCTGT+TGG 0.452894 2_1:+68793444 None:intergenic
CAAAACTGGTTAGGTCTAGA+AGG 0.452958 2_1:-68791273 Msa0220440:CDS
CAGGGTTCAAATTTGTTCAT+GGG 0.454152 2_1:-68792308 Msa0220440:CDS
AAACTCTGAGATTCCAAGCT+TGG 0.454604 2_1:+68793361 None:intergenic
CCGCAACCATCCAAAGCAAC+AGG 0.458483 2_1:-68791458 Msa0220440:CDS
ATCCAATAAAGCTAGTTTCA+AGG 0.466709 2_1:-68792748 Msa0220440:CDS
TGTGTCTTCTTCTGTTCAGC+CGG 0.478288 2_1:-68793534 Msa0220440:five_prime_UTR
GCTGAAGACCAAAACTGGTT+AGG 0.483420 2_1:-68791282 Msa0220440:CDS
AGACTTCAGCTCTTTGTCCA+TGG 0.483435 2_1:+68792629 None:intergenic
CCAAACAGAATATTTAGTAA+TGG 0.485067 2_1:-68793112 Msa0220440:CDS
ATATGAATGGATAAAGAAAC+CGG 0.487229 2_1:+68793515 None:intergenic
AACCAGAAGTTTGTAGGGTG+TGG 0.487786 2_1:-68792036 Msa0220440:CDS
ATTTACGCAGCAGGGTCGTA+AGG 0.489572 2_1:-68793068 Msa0220440:CDS
GGCCGTTCTTCAGAAAATCT+AGG 0.491219 2_1:+68791219 None:intergenic
CAAGGGTAGGGTATTTGCAT+TGG 0.493438 2_1:-68792065 Msa0220440:CDS
GGGTAGGGTATTTGCATTGG+AGG 0.495563 2_1:-68792062 Msa0220440:CDS
TCAGTTCATTGTTCTTGCCT+CGG 0.500431 2_1:-68791894 Msa0220440:CDS
TGCGGTGTGTTCGATGGCCA+TGG 0.500762 2_1:-68792864 Msa0220440:CDS
TGTGTAGGAGGAGGAAAAGG+AGG 0.506060 2_1:-68791339 Msa0220440:CDS
TCTAGCTTCATTCTTAATGT+CGG 0.507918 2_1:+68791055 None:intergenic
CCTAGTTGCACGCAAAGTTA+GGG 0.510651 2_1:-68792829 Msa0220440:CDS
TAATTTATCTGCTGAGGATG+AGG 0.511259 2_1:-68792694 Msa0220440:CDS
AGCTTTCATGAAGGCATACA+AGG 0.511427 2_1:-68792652 Msa0220440:CDS
TTTGCCGTAAGCTATATGAA+TGG 0.512854 2_1:+68793502 None:intergenic
CAAGAACAATGAACTGATCT+CGG 0.513831 2_1:+68791900 None:intergenic
TTCGATGGCCATGGTCCATA+TGG 0.516140 2_1:-68792855 Msa0220440:CDS
CGTGCAACTAGGTGACCATA+TGG 0.519449 2_1:+68792840 None:intergenic
CTCGCTGCCGATGATCGACT+TGG 0.523251 2_1:+68791621 None:intergenic
GCTGTACCAGTGAGTTTACT+CGG 0.525049 2_1:+68791244 None:intergenic
ATCTTCTGCGGTGTGTTCGA+TGG 0.528423 2_1:-68792870 Msa0220440:CDS
GCTCTAGCCATTGCTAATCC+AGG 0.531526 2_1:+68791995 None:intergenic
CCCTAACTTTGCGTGCAACT+AGG 0.533155 2_1:+68792829 None:intergenic
AAGGACATGAAGCTTGAAAG+TGG 0.537072 2_1:-68792729 Msa0220440:CDS
AATCCAGGTGCATCATCAAA+AGG 0.541135 2_1:+68792010 None:intergenic
TTAATACTTTCTATAGTGAG+AGG 0.541580 2_1:-68793416 Msa0220440:five_prime_UTR
AAGTCGATCATCGGCAGCGA+GGG 0.548909 2_1:-68791619 Msa0220440:CDS
GATCTCGGTCTGTGAGAAGC+CGG 0.551873 2_1:+68791915 None:intergenic
CATTATCAGTGTATTAGAAG+AGG 0.552370 2_1:+68794076 None:intergenic
ACTTACTTCCCATACAATCA+TGG 0.552828 2_1:+68793030 None:intergenic
TATTAAATTAAATCAAATCC+GGG 0.554787 2_1:+68793761 None:intergenic
ATCAGCACCAAGTCGATCAT+CGG 0.562467 2_1:-68791628 Msa0220440:CDS
CCTGTTGCTTTGGATGGTTG+CGG 0.563383 2_1:+68791458 None:intergenic
GGATGAACCAGAAGTTTGTA+GGG 0.564180 2_1:-68792041 Msa0220440:CDS
GAAATTTCTTACACCATCCG+AGG 0.565735 2_1:+68791877 None:intergenic
ATCGGCAGCGAGGGTTCTGG+TGG 0.567760 2_1:-68791610 Msa0220440:CDS
AATCCAGTTGAGTCAGATGA+TGG 0.571512 2_1:-68791426 Msa0220440:CDS
TGGAATCTGCCGCACGAGAG+TGG 0.576177 2_1:-68791590 Msa0220440:CDS
AATGAGACATGTGTAGGAGG+AGG 0.580853 2_1:-68791348 Msa0220440:CDS
GAGAGCAATCATGGGATCCA+AGG 0.583925 2_1:-68792270 Msa0220440:CDS
TGAGAATAATTTATCTGCTG+AGG 0.584211 2_1:-68792700 Msa0220440:CDS
AGTTCGAGCCATCTTTGCGA+AGG 0.584568 2_1:-68791398 Msa0220440:CDS
TCTTGTATATTTACGCAGCA+GGG 0.584645 2_1:-68793076 Msa0220440:CDS
AATCCGATTTCAAGACAAGA+TGG 0.585814 2_1:+68793205 None:intergenic
AATTAGTTTACATTAAGCAA+AGG 0.589217 2_1:-68793597 Msa0220440:five_prime_UTR
CAATAGAAGTAATGACGGCT+CGG 0.592065 2_1:-68793233 Msa0220440:CDS
TCAGGATGCCATGATTGTAT+GGG 0.593354 2_1:-68793038 Msa0220440:CDS
GTCAATGTGAATTCTGGGGA+AGG 0.595148 2_1:-68793713 Msa0220440:five_prime_UTR
TATAATGAGACATGTGTAGG+AGG 0.600880 2_1:-68791351 Msa0220440:CDS
TTTCTGGAACTGAGAAAATG+GGG 0.601120 2_1:-68793273 Msa0220440:exon
CTGTACCTGAATTTACGCAC+CGG 0.609120 2_1:-68791934 Msa0220440:CDS
TTTACGCAGCAGGGTCGTAA+GGG 0.610094 2_1:-68793067 Msa0220440:CDS
CATGAAGGCATACAAGGCCA+TGG 0.610791 2_1:-68792646 Msa0220440:CDS
GCGTAAATATACAAGAAGAC+CGG 0.614390 2_1:+68793083 None:intergenic
ACAAGCAATAGAAGTAATGA+CGG 0.615043 2_1:-68793238 Msa0220440:CDS
GAGTCTGTCGATGTTGAAGA+CGG 0.621885 2_1:-68791315 Msa0220440:CDS
ACATGTCTCATTATATTCGG+AGG 0.626995 2_1:+68791359 None:intergenic
TATTTAGTAATGGAAAGAGC+CGG 0.627184 2_1:-68793102 Msa0220440:CDS
TAGTGGAAGCACATCTGTGA+CGG 0.631139 2_1:-68792583 Msa0220440:CDS
CTGTACCAGTGAGTTTACTC+GGG 0.631787 2_1:+68791245 None:intergenic
GAATATAATGAGACATGTGT+AGG 0.637435 2_1:-68791354 Msa0220440:CDS
TGATCAACCGAGAATCAAAG+AGG 0.638174 2_1:+68794156 None:intergenic
GGTGACTCGAGAGCAATCAT+GGG 0.645921 2_1:-68792278 Msa0220440:CDS
GTGGAGAGAAGCTTTCATGA+AGG 0.651868 2_1:-68792661 Msa0220440:CDS
GCTAGTTTCCAACAGCAACA+AGG 0.652102 2_1:-68793452 Msa0220440:five_prime_UTR
TCAGAAGATGCGATCTTCTG+CGG 0.654370 2_1:-68792882 Msa0220440:CDS
CAAGTCGATCATCGGCAGCG+AGG 0.659177 2_1:-68791620 Msa0220440:CDS
ATGGCTTAATCTATCCAACA+AGG 0.660593 2_1:+68794032 None:intergenic
TGTCTTGAAATCGGATTGTG+TGG 0.666044 2_1:-68793199 Msa0220440:CDS
TGTCACCCGAGTAAACTCAC+TGG 0.671131 2_1:-68791250 Msa0220440:CDS
CATGAAGCTTGAAAGTGGAG+AGG 0.671148 2_1:-68792724 Msa0220440:CDS
ACATTGACCTCATCGAAATG+CGG 0.674333 2_1:+68793728 None:intergenic
AGGTATCCGACAAATAACAT+TGG 0.677287 2_1:+68794176 None:intergenic
AGGATGAGGTGAATTCTATG+TGG 0.681140 2_1:-68792680 Msa0220440:CDS
TGATGCACCTGGATTAGCAA+TGG 0.684010 2_1:-68792002 Msa0220440:CDS
TGAGGTCAATGTGAATTCTG+GGG 0.688531 2_1:-68793717 Msa0220440:five_prime_UTR
CTAGGTGACCATATGGACCA+TGG 0.689718 2_1:+68792847 None:intergenic
TTTAAGTTTCCACTCTCGTG+CGG 0.690625 2_1:+68791581 None:intergenic
TCAAACTCCGCATTTCGATG+AGG 0.703059 2_1:-68793735 Msa0220440:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTAATATTTAATATATA+TGG + chr2_1:68791684-68791703 None:intergenic 0.0%
!! AAAAAACATAAATTTAAACA+TGG + chr2_1:68793082-68793101 None:intergenic 10.0%
!! TCATAAATATTATTTATATG+AGG - chr2_1:68793450-68793469 Msa0220440:five_prime_UTR 10.0%
!!! AAGAAGAATTATTTTTTATT+TGG - chr2_1:68794112-68794131 Msa0220440:five_prime_UTR 10.0%
!!! ATAAATGTTTTATTCTCATT+TGG - chr2_1:68791300-68791319 Msa0220440:CDS 15.0%
!! TATTAAATTAAATCAAATCC+GGG + chr2_1:68791482-68791501 None:intergenic 15.0%
!! CTATTAAATTAAATCAAATC+CGG + chr2_1:68791483-68791502 None:intergenic 15.0%
!!! TTTTGATTTTTGTTTTCTTT+AGG - chr2_1:68792327-68792346 Msa0220440:intron 15.0%
!!! TATATGTTTTCTTTTGTATA+AGG - chr2_1:68792871-68792890 Msa0220440:CDS 15.0%
!!! ATATGTTTTCTTTTGTATAA+GGG - chr2_1:68792872-68792891 Msa0220440:CDS 15.0%
!!! TTTTACCTAAGAAATTTTCA+TGG + chr2_1:68791230-68791249 None:intergenic 20.0%
!!! AATTAGTTTACATTAAGCAA+AGG - chr2_1:68791643-68791662 Msa0220440:CDS 20.0%
!! AGAAAACAAAAATCAAAACT+AGG + chr2_1:68792325-68792344 None:intergenic 20.0%
!!! AAAAACGAAAGCAATTTTAT+CGG + chr2_1:68792445-68792464 None:intergenic 20.0%
!! AAAGAAAACATATACAGAAA+AGG + chr2_1:68792865-68792884 None:intergenic 20.0%
!! TGATGCAATAATATTCTTAT+AGG - chr2_1:68793128-68793147 Msa0220440:CDS 20.0%
!! TTGTAATGCTTATAACAATA+CGG - chr2_1:68794082-68794101 Msa0220440:five_prime_UTR 20.0%
! AAGAAAAAGACAGAAAGAAA+AGG + chr2_1:68791124-68791143 None:intergenic 25.0%
! TTAAGCCATGAAAATTTCTT+AGG - chr2_1:68791222-68791241 Msa0220440:CDS 25.0%
! TCTAGATGTTCATTTAATCT+TGG - chr2_1:68791386-68791405 Msa0220440:CDS 25.0%
! CACACAAATTTAGATATTTG+TGG + chr2_1:68791415-68791434 None:intergenic 25.0%
! ATATGAATGGATAAAGAAAC+CGG + chr2_1:68791728-68791747 None:intergenic 25.0%
! TTATCCATTCATATAGCTTA+CGG - chr2_1:68791734-68791753 Msa0220440:intron 25.0%
! TTAATACTTTCTATAGTGAG+AGG - chr2_1:68791824-68791843 Msa0220440:intron 25.0%
!!! ATCTAAAGTGTTTTTCTTTC+TGG - chr2_1:68791951-68791970 Msa0220440:CDS 25.0%
! ACTAAATATTCTGTTTGGTA+AGG + chr2_1:68792126-68792145 None:intergenic 25.0%
! CCATTACTAAATATTCTGTT+TGG + chr2_1:68792131-68792150 None:intergenic 25.0%
! CCAAACAGAATATTTAGTAA+TGG - chr2_1:68792128-68792147 Msa0220440:intron 25.0%
!! AGTAAGTATGTTTCATGTTT+TGG - chr2_1:68792226-68792245 Msa0220440:CDS 25.0%
!! GATAAAATTGCTTTCGTTTT+TGG - chr2_1:68792444-68792463 Msa0220440:intron 25.0%
!!! AATTTGGATTGTTTTTGTAG+TGG - chr2_1:68792640-68792659 Msa0220440:CDS 25.0%
!!! TCATTTTCTTCACTTTTTCA+GGG - chr2_1:68792914-68792933 Msa0220440:intron 25.0%
!! TGAAAGAATTAAAAAGTGCA+AGG - chr2_1:68793157-68793176 Msa0220440:CDS 25.0%
!! GAAAGAATTAAAAAGTGCAA+GGG - chr2_1:68793158-68793177 Msa0220440:CDS 25.0%
! TAAATATCCAACTTCGAAAA+TGG - chr2_1:68793678-68793697 Msa0220440:five_prime_UTR 25.0%
! TACACATGTCTCATTATATT+CGG + chr2_1:68793887-68793906 None:intergenic 25.0%
!!! ATAACCAATTTTCAAGAGTT+TGG + chr2_1:68794045-68794064 None:intergenic 25.0%
! TTGAAAATTGGTTATTCAGT+TGG - chr2_1:68794050-68794069 Msa0220440:intron 25.0%
! GATCTACCAATGTTATTTGT+CGG - chr2_1:68791058-68791077 Msa0220440:three_prime_UTR 30.0%
CATTATCAGTGTATTAGAAG+AGG + chr2_1:68791167-68791186 None:intergenic 30.0%
! ACTATTTTGTGTTACCTTGT+TGG - chr2_1:68791194-68791213 Msa0220440:exon 30.0%
!!! AAACTAGCTCCTTTTTTACT+CGG + chr2_1:68791776-68791795 None:intergenic 30.0%
ACAAGCAATAGAAGTAATGA+CGG - chr2_1:68792002-68792021 Msa0220440:CDS 30.0%
! TATTTAGTAATGGAAAGAGC+CGG - chr2_1:68792138-68792157 Msa0220440:intron 30.0%
! ATCCAATAAAGCTAGTTTCA+AGG - chr2_1:68792492-68792511 Msa0220440:intron 30.0%
TGAGAATAATTTATCTGCTG+AGG - chr2_1:68792540-68792559 Msa0220440:intron 30.0%
AAGATCAGCATCTATCATAT+TGG - chr2_1:68792699-68792718 Msa0220440:CDS 30.0%
TCACGAGTAATCTTGTAATT+TGG - chr2_1:68792791-68792810 Msa0220440:CDS 30.0%
ATTACGGGGTTAAAATACTA+CGG + chr2_1:68792818-68792837 None:intergenic 30.0%
!!! CTCATTTTCTTCACTTTTTC+AGG - chr2_1:68792913-68792932 Msa0220440:intron 30.0%
! AATTTGTTCATGGGCAATAT+CGG - chr2_1:68792941-68792960 Msa0220440:intron 30.0%
ATGATTTCATATGACACACT+TGG + chr2_1:68793052-68793071 None:intergenic 30.0%
! AATTAAAAAGTGCAAGGGTA+GGG - chr2_1:68793163-68793182 Msa0220440:CDS 30.0%
!!! TTGTGTGTCTATTTTTGGAT+GGG - chr2_1:68793713-68793732 Msa0220440:five_prime_UTR 30.0%
!!! TCTATTTTTGGATGGGAAAA+TGG - chr2_1:68793720-68793739 Msa0220440:five_prime_UTR 30.0%
GAATATAATGAGACATGTGT+AGG - chr2_1:68793886-68793905 Msa0220440:five_prime_UTR 30.0%
!! TTCCTAGATTTTCTGAAGAA+CGG - chr2_1:68794019-68794038 Msa0220440:five_prime_UTR 30.0%
! TCTAGCTTCATTCTTAATGT+CGG + chr2_1:68794188-68794207 None:intergenic 30.0%
AGGTATCCGACAAATAACAT+TGG + chr2_1:68791067-68791086 None:intergenic 35.0%
ATGGCTTAATCTATCCAACA+AGG + chr2_1:68791211-68791230 None:intergenic 35.0%
!!! AGTTTTAATTGCACAGTTCC+CGG - chr2_1:68791461-68791480 Msa0220440:CDS 35.0%
ATGAGGTCAATGTGAATTCT+GGG - chr2_1:68791522-68791541 Msa0220440:CDS 35.0%
TTTGCCGTAAGCTATATGAA+TGG + chr2_1:68791741-68791760 None:intergenic 35.0%
TTCTATAGTGAGAGGATCTT+TGG - chr2_1:68791832-68791851 Msa0220440:intron 35.0%
TTTACTCAATTGACCAAGCT+TGG - chr2_1:68791866-68791885 Msa0220440:CDS 35.0%
!! TCTTTCTGGAACTGAGAAAA+TGG - chr2_1:68791965-68791984 Msa0220440:CDS 35.0%
!! CTTTCTGGAACTGAGAAAAT+GGG - chr2_1:68791966-68791985 Msa0220440:CDS 35.0%
!! TTTCTGGAACTGAGAAAATG+GGG - chr2_1:68791967-68791986 Msa0220440:CDS 35.0%
ACTCCATCTTGTCTTGAAAT+CGG - chr2_1:68792032-68792051 Msa0220440:CDS 35.0%
AATCCGATTTCAAGACAAGA+TGG + chr2_1:68792038-68792057 None:intergenic 35.0%
GCGTAAATATACAAGAAGAC+CGG + chr2_1:68792160-68792179 None:intergenic 35.0%
TTCTTGTATATTTACGCAGC+AGG - chr2_1:68792163-68792182 Msa0220440:intron 35.0%
TCTTGTATATTTACGCAGCA+GGG - chr2_1:68792164-68792183 Msa0220440:intron 35.0%
ACTTACTTCCCATACAATCA+TGG + chr2_1:68792213-68792232 None:intergenic 35.0%
TCATTGAATGAAGACAGACT+TGG - chr2_1:68792271-68792290 Msa0220440:CDS 35.0%
!! GTCCTTGAAACTAGCTTTAT+TGG + chr2_1:68792497-68792516 None:intergenic 35.0%
TAATTTATCTGCTGAGGATG+AGG - chr2_1:68792546-68792565 Msa0220440:intron 35.0%
TAGTTTACTGATAAGTGAGC+AGG - chr2_1:68792760-68792779 Msa0220440:CDS 35.0%
! TCAGGGTTCAAATTTGTTCA+TGG - chr2_1:68792931-68792950 Msa0220440:intron 35.0%
! CAGGGTTCAAATTTGTTCAT+GGG - chr2_1:68792932-68792951 Msa0220440:intron 35.0%
! GAATTAAAAAGTGCAAGGGT+AGG - chr2_1:68793162-68793181 Msa0220440:CDS 35.0%
! ATTTGGCGATTTTTGCTTGA+AGG - chr2_1:68793268-68793287 Msa0220440:exon 35.0%
! GATTTTTGCTTGAAGGAGTA+TGG - chr2_1:68793275-68793294 Msa0220440:exon 35.0%
CAAGAACAATGAACTGATCT+CGG + chr2_1:68793343-68793362 None:intergenic 35.0%
CTTAAACCTTTGTCTTCGTT+CGG - chr2_1:68793536-68793555 Msa0220440:five_prime_UTR 35.0%
! TGCTGTTGTGTGTCTATTTT+TGG - chr2_1:68793708-68793727 Msa0220440:five_prime_UTR 35.0%
!!! GTTGTGTGTCTATTTTTGGA+TGG - chr2_1:68793712-68793731 Msa0220440:five_prime_UTR 35.0%
ACATGTCTCATTATATTCGG+AGG + chr2_1:68793884-68793903 None:intergenic 35.0%
TATAATGAGACATGTGTAGG+AGG - chr2_1:68793889-68793908 Msa0220440:five_prime_UTR 35.0%
TCGGATACCTCTTTGATTCT+CGG - chr2_1:68791077-68791096 Msa0220440:three_prime_UTR 40.0%
TGATCAACCGAGAATCAAAG+AGG + chr2_1:68791087-68791106 None:intergenic 40.0%
ACATTGACCTCATCGAAATG+CGG + chr2_1:68791515-68791534 None:intergenic 40.0%
GATGAGGTCAATGTGAATTC+TGG - chr2_1:68791521-68791540 Msa0220440:CDS 40.0%
TGAGGTCAATGTGAATTCTG+GGG - chr2_1:68791523-68791542 Msa0220440:CDS 40.0%
CGCATTTGACCGAGTAAAAA+AGG - chr2_1:68791764-68791783 Msa0220440:intron 40.0%
AATTCACACCTTGTTGCTGT+TGG + chr2_1:68791799-68791818 None:intergenic 40.0%
!! AAACTCTGAGATTCCAAGCT+TGG + chr2_1:68791882-68791901 None:intergenic 40.0%
CAATAGAAGTAATGACGGCT+CGG - chr2_1:68792007-68792026 Msa0220440:CDS 40.0%
TGTCTTGAAATCGGATTGTG+TGG - chr2_1:68792041-68792060 Msa0220440:CDS 40.0%
ATCAGGATGCCATGATTGTA+TGG - chr2_1:68792201-68792220 Msa0220440:intron 40.0%
TCAGGATGCCATGATTGTAT+GGG - chr2_1:68792202-68792221 Msa0220440:intron 40.0%
TGAAGACAGACTTGGAATCT+TGG - chr2_1:68792279-68792298 Msa0220440:CDS 40.0%
! CGAAAGCAATTTTATCGGCA+AGG + chr2_1:68792440-68792459 None:intergenic 40.0%
AAGGACATGAAGCTTGAAAG+TGG - chr2_1:68792511-68792530 Msa0220440:intron 40.0%
AGGATGAGGTGAATTCTATG+TGG - chr2_1:68792560-68792579 Msa0220440:intron 40.0%
! AGCTTTCATGAAGGCATACA+AGG - chr2_1:68792588-68792607 Msa0220440:CDS 40.0%
GCTGAAGTCTCATGCAAATT+TGG - chr2_1:68792624-68792643 Msa0220440:CDS 40.0%
AACACACGCGATTTGATTAC+GGG + chr2_1:68792833-68792852 None:intergenic 40.0%
GAACACACGCGATTTGATTA+CGG + chr2_1:68792834-68792853 None:intergenic 40.0%
TATGACACACTTGGCAAATC+AGG + chr2_1:68793043-68793062 None:intergenic 40.0%
AGGATGAACCAGAAGTTTGT+AGG - chr2_1:68793198-68793217 Msa0220440:CDS 40.0%
GGATGAACCAGAAGTTTGTA+GGG - chr2_1:68793199-68793218 Msa0220440:CDS 40.0%
!!! TTACCTTTTGATGATGCACC+TGG - chr2_1:68793227-68793246 Msa0220440:CDS 40.0%
AATCCAGGTGCATCATCAAA+AGG + chr2_1:68793233-68793252 None:intergenic 40.0%
! TTAGCAATGGCTAGAGCATT+TGG - chr2_1:68793251-68793270 Msa0220440:CDS 40.0%
TCAGTTCATTGTTCTTGCCT+CGG - chr2_1:68793346-68793365 Msa0220440:five_prime_UTR 40.0%
GAAATTTCTTACACCATCCG+AGG + chr2_1:68793366-68793385 None:intergenic 40.0%
! TTTAAGTTTCCACTCTCGTG+CGG + chr2_1:68793662-68793681 None:intergenic 40.0%
! CAGTCATCCATTTTCGAAGT+TGG + chr2_1:68793688-68793707 None:intergenic 40.0%
AATCCAGTTGAGTCAGATGA+TGG - chr2_1:68793814-68793833 Msa0220440:five_prime_UTR 40.0%
! CTTCTAGACCTAACCAGTTT+TGG + chr2_1:68793969-68793988 None:intergenic 40.0%
CAAAACTGGTTAGGTCTAGA+AGG - chr2_1:68793967-68793986 Msa0220440:five_prime_UTR 40.0%
ACGGCCAAACTCTTGAAAAT+TGG - chr2_1:68794038-68794057 Msa0220440:five_prime_UTR 40.0%
TCAAACTCCGCATTTCGATG+AGG - chr2_1:68791505-68791524 Msa0220440:CDS 45.0%
! GTCAATGTGAATTCTGGGGA+AGG - chr2_1:68791527-68791546 Msa0220440:CDS 45.0%
TGTGTCTTCTTCTGTTCAGC+CGG - chr2_1:68791706-68791725 Msa0220440:intron 45.0%
! GCTAGTTTCCAACAGCAACA+AGG - chr2_1:68791788-68791807 Msa0220440:intron 45.0%
!! GTGAGAGGATCTTTGGAATC+AGG - chr2_1:68791839-68791858 Msa0220440:intron 45.0%
TCAGAAGATGCGATCTTCTG+CGG - chr2_1:68792358-68792377 Msa0220440:intron 45.0%
ACCTAGTTGCACGCAAAGTT+AGG - chr2_1:68792410-68792429 Msa0220440:intron 45.0%
CCTAGTTGCACGCAAAGTTA+GGG - chr2_1:68792411-68792430 Msa0220440:intron 45.0%
CATGAAGCTTGAAAGTGGAG+AGG - chr2_1:68792516-68792535 Msa0220440:intron 45.0%
GTGGAGAGAAGCTTTCATGA+AGG - chr2_1:68792579-68792598 Msa0220440:CDS 45.0%
AGACTTCAGCTCTTTGTCCA+TGG + chr2_1:68792614-68792633 None:intergenic 45.0%
! TAGTGGAAGCACATCTGTGA+CGG - chr2_1:68792657-68792676 Msa0220440:CDS 45.0%
ATCTGTGACGGTAGTGAAAC+AGG - chr2_1:68792669-68792688 Msa0220440:CDS 45.0%
ACACACGCGATTTGATTACG+GGG + chr2_1:68792832-68792851 None:intergenic 45.0%
CATCAAGTCATTGCTGTCCT+TGG + chr2_1:68792990-68793009 None:intergenic 45.0%
CAAGGACAGCAATGACTTGA+TGG - chr2_1:68792988-68793007 Msa0220440:intron 45.0%
! CAAGGGTAGGGTATTTGCAT+TGG - chr2_1:68793175-68793194 Msa0220440:CDS 45.0%
AACCAGAAGTTTGTAGGGTG+TGG - chr2_1:68793204-68793223 Msa0220440:CDS 45.0%
AACCACACCCTACAAACTTC+TGG + chr2_1:68793209-68793228 None:intergenic 45.0%
!! TGATGCACCTGGATTAGCAA+TGG - chr2_1:68793238-68793257 Msa0220440:CDS 45.0%
CTGTACCTGAATTTACGCAC+CGG - chr2_1:68793306-68793325 Msa0220440:five_prime_UTR 45.0%
TTCATTGTTCTTGCCTCGGA+TGG - chr2_1:68793350-68793369 Msa0220440:five_prime_UTR 45.0%
!! GGATGTTTTGAGCAATGACG+AGG - chr2_1:68793573-68793592 Msa0220440:five_prime_UTR 45.0%
!! TGTTTTGAGCAATGACGAGG+TGG - chr2_1:68793576-68793595 Msa0220440:five_prime_UTR 45.0%
ATCAGCACCAAGTCGATCAT+CGG - chr2_1:68793612-68793631 Msa0220440:five_prime_UTR 45.0%
!! TGAATGCCTGTTGCTTTGGA+TGG + chr2_1:68793791-68793810 None:intergenic 45.0%
ATCCAAAGCAACAGGCATTC+AGG - chr2_1:68793790-68793809 Msa0220440:five_prime_UTR 45.0%
TGACCATCATCTGACTCAAC+TGG + chr2_1:68793820-68793839 None:intergenic 45.0%
GTGAAGTTCCTTCGCAAAGA+TGG + chr2_1:68793853-68793872 None:intergenic 45.0%
AATGAGACATGTGTAGGAGG+AGG - chr2_1:68793892-68793911 Msa0220440:five_prime_UTR 45.0%
ACATGTGTAGGAGGAGGAAA+AGG - chr2_1:68793898-68793917 Msa0220440:five_prime_UTR 45.0%
GAGTCTGTCGATGTTGAAGA+CGG - chr2_1:68793925-68793944 Msa0220440:five_prime_UTR 45.0%
CATCAGCTGAAGACCAAAAC+TGG - chr2_1:68793953-68793972 Msa0220440:five_prime_UTR 45.0%
GCTGAAGACCAAAACTGGTT+AGG - chr2_1:68793958-68793977 Msa0220440:five_prime_UTR 45.0%
CTGTACCAGTGAGTTTACTC+GGG + chr2_1:68793998-68794017 None:intergenic 45.0%
GCTGTACCAGTGAGTTTACT+CGG + chr2_1:68793999-68794018 None:intergenic 45.0%
GGCCGTTCTTCAGAAAATCT+AGG + chr2_1:68794024-68794043 None:intergenic 45.0%
ATTTACGCAGCAGGGTCGTA+AGG - chr2_1:68792172-68792191 Msa0220440:intron 50.0%
TTTACGCAGCAGGGTCGTAA+GGG - chr2_1:68792173-68792192 Msa0220440:intron 50.0%
!! GGGTCGTAAGGGTGTTAATC+AGG - chr2_1:68792184-68792203 Msa0220440:intron 50.0%
ATCTTCTGCGGTGTGTTCGA+TGG - chr2_1:68792370-68792389 Msa0220440:intron 50.0%
TTCGATGGCCATGGTCCATA+TGG - chr2_1:68792385-68792404 Msa0220440:intron 50.0%
CTAGGTGACCATATGGACCA+TGG + chr2_1:68792396-68792415 None:intergenic 50.0%
CGTGCAACTAGGTGACCATA+TGG + chr2_1:68792403-68792422 None:intergenic 50.0%
CCCTAACTTTGCGTGCAACT+AGG + chr2_1:68792414-68792433 None:intergenic 50.0%
! CATGAAGGCATACAAGGCCA+TGG - chr2_1:68792594-68792613 Msa0220440:CDS 50.0%
GGTGACTCGAGAGCAATCAT+GGG - chr2_1:68792962-68792981 Msa0220440:intron 50.0%
GAGAGCAATCATGGGATCCA+AGG - chr2_1:68792970-68792989 Msa0220440:intron 50.0%
! GGGTAGGGTATTTGCATTGG+AGG - chr2_1:68793178-68793197 Msa0220440:CDS 50.0%
GCTCTAGCCATTGCTAATCC+AGG + chr2_1:68793248-68793267 None:intergenic 50.0%
AGAAGCCGGTGCGTAAATTC+AGG + chr2_1:68793314-68793333 None:intergenic 50.0%
CAGAGACCGAACGAAGACAA+AGG + chr2_1:68793545-68793564 None:intergenic 50.0%
TCGTTCGGTCTCTGCAGATA+TGG - chr2_1:68793551-68793570 Msa0220440:five_prime_UTR 50.0%
CGTTCGGTCTCTGCAGATAT+GGG - chr2_1:68793552-68793571 Msa0220440:five_prime_UTR 50.0%
!! CCTGTTGCTTTGGATGGTTG+CGG + chr2_1:68793785-68793804 None:intergenic 50.0%
! TGCCTGAATGCCTGTTGCTT+TGG + chr2_1:68793795-68793814 None:intergenic 50.0%
AGTTCGAGCCATCTTTGCGA+AGG - chr2_1:68793842-68793861 Msa0220440:five_prime_UTR 50.0%
TGTGTAGGAGGAGGAAAAGG+AGG - chr2_1:68793901-68793920 Msa0220440:five_prime_UTR 50.0%
TGTCACCCGAGTAAACTCAC+TGG - chr2_1:68793990-68794009 Msa0220440:five_prime_UTR 50.0%
CGGTGACTCGAGAGCAATCA+TGG - chr2_1:68792961-68792980 Msa0220440:intron 55.0%
GATCTCGGTCTGTGAGAAGC+CGG + chr2_1:68793328-68793347 None:intergenic 55.0%
AAGTCGATCATCGGCAGCGA+GGG - chr2_1:68793621-68793640 Msa0220440:five_prime_UTR 55.0%
CCGCAACCATCCAAAGCAAC+AGG - chr2_1:68793782-68793801 Msa0220440:five_prime_UTR 55.0%
TGCGGTGTGTTCGATGGCCA+TGG - chr2_1:68792376-68792395 Msa0220440:intron 60.0%
CTCGCTGCCGATGATCGACT+TGG + chr2_1:68793622-68793641 None:intergenic 60.0%
CAAGTCGATCATCGGCAGCG+AGG - chr2_1:68793620-68793639 Msa0220440:five_prime_UTR 60.0%
ATCATCGGCAGCGAGGGTTC+TGG - chr2_1:68793627-68793646 Msa0220440:five_prime_UTR 60.0%
TGGAATCTGCCGCACGAGAG+TGG - chr2_1:68793650-68793669 Msa0220440:five_prime_UTR 60.0%
! ATCGGCAGCGAGGGTTCTGG+TGG - chr2_1:68793630-68793649 Msa0220440:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 68791051 68794211 68791051 ID=Msa0220440;Name=Msa0220440
chr2_1 mRNA 68791051 68794211 68791051 ID=Msa0220440-mRNA-1;Parent=Msa0220440;Name=Msa0220440-mRNA-1;_AED=0.12;_eAED=0.12;_QI=911|0.8|0.83|1|0.8|0.66|6|158|479
chr2_1 exon 68791051 68791694 68791051 ID=Msa0220440-mRNA-1:exon:19465;Parent=Msa0220440-mRNA-1
chr2_1 exon 68791862 68792112 68791862 ID=Msa0220440-mRNA-1:exon:19464;Parent=Msa0220440-mRNA-1
chr2_1 exon 68792210 68792327 68792210 ID=Msa0220440-mRNA-1:exon:19463;Parent=Msa0220440-mRNA-1
chr2_1 exon 68792572 68792913 68792572 ID=Msa0220440-mRNA-1:exon:19462;Parent=Msa0220440-mRNA-1
chr2_1 exon 68793036 68794059 68793036 ID=Msa0220440-mRNA-1:exon:19461;Parent=Msa0220440-mRNA-1
chr2_1 exon 68794082 68794211 68794082 ID=Msa0220440-mRNA-1:exon:19460;Parent=Msa0220440-mRNA-1
chr2_1 five_prime_UTR 68794082 68794211 68794082 ID=Msa0220440-mRNA-1:five_prime_utr;Parent=Msa0220440-mRNA-1
chr2_1 five_prime_UTR 68793279 68794059 68793279 ID=Msa0220440-mRNA-1:five_prime_utr;Parent=Msa0220440-mRNA-1
chr2_1 CDS 68793036 68793278 68793036 ID=Msa0220440-mRNA-1:cds;Parent=Msa0220440-mRNA-1
chr2_1 CDS 68792572 68792913 68792572 ID=Msa0220440-mRNA-1:cds;Parent=Msa0220440-mRNA-1
chr2_1 CDS 68792210 68792327 68792210 ID=Msa0220440-mRNA-1:cds;Parent=Msa0220440-mRNA-1
chr2_1 CDS 68791862 68792112 68791862 ID=Msa0220440-mRNA-1:cds;Parent=Msa0220440-mRNA-1
chr2_1 CDS 68791209 68791694 68791209 ID=Msa0220440-mRNA-1:cds;Parent=Msa0220440-mRNA-1
chr2_1 three_prime_UTR 68791051 68791208 68791051 ID=Msa0220440-mRNA-1:three_prime_utr;Parent=Msa0220440-mRNA-1
Gene Sequence

>Msa0220440

ATGGGGTGTTGTGTCTCAACAAGCAATAGAAGTAATGACGGCTCGGTTACTCCATCTTGTCTTGAAATCGGATTGTGTGGTCAAAAGAAAGCAAGAAGAACATTCTCTGATCATGTTGTCTCTCTGAACAAGTTAGCATCCTTACCAAACAGAATATTTAGTAATGGAAAGAGCCGGTCTTCTTGTATATTTACGCAGCAGGGTCGTAAGGGTGTTAATCAGGATGCCATGATTGTATGGGAAGATTTTGTGTCAGAAGATGCGATCTTCTGCGGTGTGTTCGATGGCCATGGTCCATATGGTCACCTAGTTGCACGCAAAGTTAGGGATGCCTTGCCGATAAAATTGCTTTCGTTTTTGGATTCATCTGAGTCGAAGCGAAATGAATCCAATAAAGCTAGTTTCAAGGACATGAAGCTTGAAAGTGGAGAGGCTGAGAATAATTTATCTGCTGAGGATGAGGTGAATTCTATGTGGAGAGAAGCTTTCATGAAGGCATACAAGGCCATGGACAAAGAGCTGAAGTCTCATGCAAATTTGGATTGTTTTTGTAGTGGAAGCACATCTGTGACGGTAGTGAAACAGGGTTCAAATTTGTTCATGGGCAATATCGGTGACTCGAGAGCAATCATGGGATCCAAGGACAGCAATGACTTGATGGTTGCAATTCAGCTGACAGTTGATTTAAAACCTGATTTGCCAAGAGAAGCTGAAAGAATTAAAAAGTGCAAGGGTAGGGTATTTGCATTGGAGGATGAACCAGAAGTTTGTAGGGTGTGGTTACCTTTTGATGATGCACCTGGATTAGCAATGGCTAGAGCATTTGGCGATTTTTGCTTGAAGGAGTATGGTGTGATTTCTGTACCTGAATTTACGCACCGGCTTCTCACAGACCGAGATCAGTTCATTGTTCTTGCCTCGGATGGTGTAAGAAATTTCAATTTCATATCTACTATATGGGATGTTTTGAGCAATGACGAGGTGGTAGAAATAGTATCATCAGCACCAAGTCGATCATCGGCAGCGAGGGTTCTGGTGGAATCTGCCGCACGAGAGTGGAAACTTAAATATCCAACTTCGAAAATGGATGACTGTGCTGTTGTGTGTCTATTTTTGGATGGGAAAATGGACTCAGAAGAATCTGATTGTGATGAACAATGCTATTCTTCCGCAACCATCCAAAGCAACAGGCATTCAGGCAATCCAGTTGAGTCAGATGATGGTCAAAAGTTCGAGCCATCTTTGCGAAGGAACTTCACTGTGAGAACCTCCGAATATAATGAGACATGTGTAGGAGGAGGAAAAGGAGGAGAGTCTGTCGATGTTGAAGACGGAACATCATCAGCTGAAGACCAAAACTGGTTAGGTCTAGAAGGTGTCACCCGAGTAAACTCACTGGTACAGCTTCCTAGATTTTCTGAAGAACGGCCAAACTCTTGA
Protein sequence

>Msa0220440

MGCCVSTSNRSNDGSVTPSCLEIGLCGQKKARRTFSDHVVSLNKLASLPNRIFSNGKSRSSCIFTQQGRKGVNQDAMIVWEDFVSEDAIFCGVFDGHGPYGHLVARKVRDALPIKLLSFLDSSESKRNESNKASFKDMKLESGEAENNLSAEDEVNSMWREAFMKAYKAMDKELKSHANLDCFCSGSTSVTVVKQGSNLFMGNIGDSRAIMGSKDSNDLMVAIQLTVDLKPDLPREAERIKKCKGRVFALEDEPEVCRVWLPFDDAPGLAMARAFGDFCLKEYGVISVPEFTHRLLTDRDQFIVLASDGVRNFNFISTIWDVLSNDEVVEIVSSAPSRSSAARVLVESAAREWKLKYPTSKMDDCAVVCLFLDGKMDSEESDCDEQCYSSATIQSNRHSGNPVESDDGQKFEPSLRRNFTVRTSEYNETCVGGGKGGESVDVEDGTSSAEDQNWLGLEGVTRVNSLVQLPRFSEERPNS*