AlfalfaGEDB | Msa0783740

M. sativa cultivar zhongmu-4 / Msa0783740

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004521 GO:0004536 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006308 GO:0006401 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0019439 GO:0034641 GO:0034655 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044464 GO:0046483 GO:0046700 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0140097 GO:0140098 GO:1901360 GO:1901361 GO:1901575
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0783740	XP_003616736.1	83.962	106	7	2	1	96	1	106	3.25e-53	179

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0783740	sp\|Q39635\|Y38K_CAPSE	53.719	121	37	3	1	103	1	120	4.37e-35	125

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0783740	G7K7B4	83.962	106	7	2	1	96	1	106	1.55e-53	179

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0022550	Msa0783740	0.803031	4.232802e-49	-8.615850e-47
Msa0022750	Msa0783740	-0.801858	7.383651e-49	-8.615850e-47
Msa0048000	Msa0783740	0.810486	1.125601e-50	-8.615850e-47
Msa0068680	Msa0783740	0.802077	6.658191e-49	-8.615850e-47
Msa0077450	Msa0783740	0.806075	9.808267e-50	-8.615850e-47
Msa0622650	Msa0783740	0.811184	7.946720e-51	-8.615850e-47
Msa0693380	Msa0783740	0.820841	5.541140e-53	-8.615850e-47
Msa0723990	Msa0783740	0.801794	7.612044e-49	-8.615850e-47
Msa0750240	Msa0783740	0.800052	1.726631e-48	-8.615850e-47
Msa0751260	Msa0783740	0.803013	4.268593e-49	-8.615850e-47
Msa0760130	Msa0783740	0.805097	1.573125e-49	-8.615850e-47
Msa0783740	Msa0795070	0.815969	7.040126e-52	-8.615850e-47
Msa0783740	Msa0830510	0.800486	1.409213e-48	-8.615850e-47
Msa0783740	Msa0926890	0.802333	5.895567e-49	-8.615850e-47
Msa0783740	Msa0936210	0.802226	6.203557e-49	-8.615850e-47
Msa0783740	Msa0939000	0.812343	4.447102e-51	-8.615850e-47
Msa0783740	Msa0975940	0.821983	3.021093e-53	-8.615850e-47
Msa0783740	Msa1031930	0.809228	2.099429e-50	-8.615850e-47
Msa0783740	Msa1040520	0.810730	9.967695e-51	-8.615850e-47
Msa0783740	Msa1080160	0.803598	3.230014e-49	-8.615850e-47
Msa0783740	Msa1082500	0.811028	8.590754e-51	-8.615850e-47
Msa0783740	Msa1113010	0.803789	2.947668e-49	-8.615850e-47
Msa0783740	Msa1127770	0.827997	1.148244e-54	-8.615850e-47
Msa0783740	Msa1158310	0.811356	7.293093e-51	-8.615850e-47
Msa0783740	Msa1379850	0.814287	1.663422e-51	-8.615850e-47
Msa0783740	Msa1418260	0.805830	1.104419e-49	-8.615850e-47
Msa0783740	Msa1459050	0.801786	7.639368e-49	-8.615850e-47
Msa0123300	Msa0783740	0.801483	8.815534e-49	-8.615850e-47
Msa0143560	Msa0783740	0.802011	6.868511e-49	-8.615850e-47
Msa0170140	Msa0783740	0.806408	8.348423e-50	-8.615850e-47
Msa0187030	Msa0783740	0.827096	1.889670e-54	-8.615850e-47
Msa0436370	Msa0783740	0.813308	2.733320e-51	-8.615850e-47
Msa0469160	Msa0783740	0.800795	1.218695e-48	-8.615850e-47
Msa0260810	Msa0783740	0.805882	1.077073e-49	-8.615850e-47
Msa0264780	Msa0783740	0.816023	6.846250e-52	-8.615850e-47
Msa0351380	Msa0783740	0.803111	4.073416e-49	-8.615850e-47
Msa0484780	Msa0783740	0.816098	6.588633e-52	-8.615850e-47
Msa0526940	Msa0783740	0.805002	1.647513e-49	-8.615850e-47
Msa0572480	Msa0783740	0.800807	1.211982e-48	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0783740	MtrunA17_Chr5g0437911	83.962	106	7	2	1	96	1	106	2.99e-57	179
Msa0783740	MtrunA17_Chr5g0437901	79.048	105	13	1	1	96	1	105	1.13e-53	170

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0783740	AT2G40410.1	54.955	111	35	2	1	96	1	111	2.47e-35	120
Msa0783740	AT2G40410.2	54.955	111	35	2	1	96	1	111	5.37e-34	120
Msa0783740	AT3G56170.1	48.571	105	41	3	1	96	1	101	7.24e-24	93.2

Find 17 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTGTTATTCTGACATTAGA+TGG	0.318151	5_2:+67878010	Msa0783740:CDS
AATTAAGTAGAATTGAAGTT+TGG	0.347604	5_2:-67878069	None:intergenic
ACAAGTCTAGCTGCTTCTTC+AGG	0.380928	5_2:-67877992	None:intergenic
ACATGTCTGCTTAGTCCTTC+AGG	0.390784	5_2:-67877668	None:intergenic
GCTGCTTCTTCAGGTGTCCT+AGG	0.424973	5_2:-67877983	None:intergenic
AGGTATAGAAAGTTAATAGA+TGG	0.432563	5_2:+67877938	Msa0783740:intron
TCTTCAGGTGTCCTAGGTGG+TGG	0.500714	5_2:-67877977	None:intergenic
GTTTGCAGCAATTGGCATAG+AGG	0.516813	5_2:-67877421	None:intergenic
GTTAATAGATGGTTGGAAAG+AGG	0.525689	5_2:+67877949	Msa0783740:CDS
ATAGAAAGTTAATAGATGGT+TGG	0.548270	5_2:+67877942	Msa0783740:CDS
TAACTTTGAAAACACCTCCC+AGG	0.556186	5_2:+67877508	Msa0783740:CDS
AAGAGGCAAGGCCACCACCT+AGG	0.618732	5_2:+67877966	Msa0783740:CDS
TAGATGGTTGGAAAGAGGCA+AGG	0.625445	5_2:+67877954	Msa0783740:CDS
GCTTCTTCAGGTGTCCTAGG+TGG	0.638052	5_2:-67877980	None:intergenic
AGCAAGTGCTGAAACACTAA+CGG	0.658395	5_2:-67877474	None:intergenic
AACAAAATAGGTTACCTGGG+AGG	0.716149	5_2:-67877522	None:intergenic
AAGTGCTGAAACACTAACGG+TGG	0.743011	5_2:-67877471	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTATATTGTTTTGAATTTGA+AGG	+	chr5_2:67877918-67877937	Msa0783740:intron	15.0%
!!!	ATGTTTTTGAGTAACAAAAT+AGG	-	chr5_2:67877537-67877556	None:intergenic	20.0%
!!!	AATATAAGCAAAGCATTAAA+GGG	-	chr5_2:67877905-67877924	None:intergenic	20.0%
!!	AATTAAGTAGAATTGAAGTT+TGG	-	chr5_2:67878072-67878091	None:intergenic	20.0%
!!	TGGTAAAAAGAGTAGAATTT+TGG	-	chr5_2:67877589-67877608	None:intergenic	25.0%
!	TATATGCACATGTTACAAAA+TGG	-	chr5_2:67877618-67877637	None:intergenic	25.0%
!	GTAAAAGAAAATTACACTGA+CGG	+	chr5_2:67877851-67877870	Msa0783740:intron	25.0%
!!	GCATTAAAGGGTATAATAAT+TGG	-	chr5_2:67877893-67877912	None:intergenic	25.0%
!!	CAATATAAGCAAAGCATTAA+AGG	-	chr5_2:67877906-67877925	None:intergenic	25.0%
!	AGGTATAGAAAGTTAATAGA+TGG	+	chr5_2:67877938-67877957	Msa0783740:intron	25.0%
!	ATAGAAAGTTAATAGATGGT+TGG	+	chr5_2:67877942-67877961	Msa0783740:CDS	25.0%
!	ACATAAAAAAGAAGATGTTG+AGG	+	chr5_2:67878033-67878052	Msa0783740:CDS	25.0%
!	TGATGATCATCAGATGTTTT+TGG	-	chr5_2:67877446-67877465	None:intergenic	30.0%
	AGTAACAAAATAGGTTACCT+GGG	-	chr5_2:67877528-67877547	None:intergenic	30.0%
!!	GTAGAATTTTGGTAAGTTTG+AGG	-	chr5_2:67877578-67877597	None:intergenic	30.0%
!!!	AATCTTTTTTAGGTTCCTGA+AGG	+	chr5_2:67877653-67877672	Msa0783740:intron	30.0%
!	ACATGTTGTGTCTTCTAAAA+AGG	+	chr5_2:67877685-67877704	Msa0783740:intron	30.0%
	ACAGTGCATAGAAATTAATC+TGG	-	chr5_2:67877767-67877786	None:intergenic	30.0%
!!	CTTGTTATTCTGACATTAGA+TGG	+	chr5_2:67878010-67878029	Msa0783740:CDS	30.0%
!!!	TGTTTTTGGTTTGCAGCAAT+TGG	-	chr5_2:67877432-67877451	None:intergenic	35.0%
	GAGTAACAAAATAGGTTACC+TGG	-	chr5_2:67877529-67877548	None:intergenic	35.0%
	TGAGGTGAAAAACACAATTG+AGG	-	chr5_2:67877560-67877579	None:intergenic	35.0%
	TGCACATGTTACAAAATGGT+GGG	-	chr5_2:67877614-67877633	None:intergenic	35.0%
	ATGCACATGTTACAAAATGG+TGG	-	chr5_2:67877615-67877634	None:intergenic	35.0%
	GTTAATAGATGGTTGGAAAG+AGG	+	chr5_2:67877949-67877968	Msa0783740:CDS	35.0%
!!	AGCAAGTGCTGAAACACTAA+CGG	-	chr5_2:67877477-67877496	None:intergenic	40.0%
!	TAACTTTGAAAACACCTCCC+AGG	+	chr5_2:67877508-67877527	Msa0783740:CDS	40.0%
	AACAAAATAGGTTACCTGGG+AGG	-	chr5_2:67877525-67877544	None:intergenic	40.0%
!	ATGTTACAAAATGGTGGGGT+TGG	-	chr5_2:67877609-67877628	None:intergenic	40.0%
	GCACATGTTACAAAATGGTG+GGG	-	chr5_2:67877613-67877632	None:intergenic	40.0%
	CTAAAAAGGCTCAAGCTAAG+TGG	+	chr5_2:67877699-67877718	Msa0783740:intron	40.0%
	GTTTGCAGCAATTGGCATAG+AGG	-	chr5_2:67877424-67877443	None:intergenic	45.0%
!!	AAGTGCTGAAACACTAACGG+TGG	-	chr5_2:67877474-67877493	None:intergenic	45.0%
	ACATGTCTGCTTAGTCCTTC+AGG	-	chr5_2:67877671-67877690	None:intergenic	45.0%
	TAGATGGTTGGAAAGAGGCA+AGG	+	chr5_2:67877954-67877973	Msa0783740:CDS	45.0%
	ACAAGTCTAGCTGCTTCTTC+AGG	-	chr5_2:67877995-67878014	None:intergenic	45.0%
!!!	TTTATTTTTTAATCTTTTTT+AGG	+	chr5_2:67877643-67877662	Msa0783740:intron	5.0%
	TCTTCAGGTGTCCTAGGTGG+TGG	-	chr5_2:67877980-67877999	None:intergenic	55.0%
	GCTTCTTCAGGTGTCCTAGG+TGG	-	chr5_2:67877983-67878002	None:intergenic	55.0%
	GCTGCTTCTTCAGGTGTCCT+AGG	-	chr5_2:67877986-67878005	None:intergenic	55.0%
	AAGAGGCAAGGCCACCACCT+AGG	+	chr5_2:67877966-67877985	Msa0783740:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	67877401	67878117	67877401	ID=Msa0783740;Name=Msa0783740
chr5_2	mRNA	67877401	67878117	67877401	ID=Msa0783740-mRNA-1;Parent=Msa0783740;Name=Msa0783740-mRNA-1;_AED=0.40;_eAED=0.51;_QI=0\|0.5\|0\|1\|1\|1\|3\|0\|111
chr5_2	exon	67877401	67877529	67877401	ID=Msa0783740-mRNA-1:exon:20334;Parent=Msa0783740-mRNA-1
chr5_2	exon	67877665	67877693	67877665	ID=Msa0783740-mRNA-1:exon:20335;Parent=Msa0783740-mRNA-1
chr5_2	exon	67877940	67878117	67877940	ID=Msa0783740-mRNA-1:exon:20336;Parent=Msa0783740-mRNA-1
chr5_2	CDS	67877401	67877529	67877401	ID=Msa0783740-mRNA-1:cds;Parent=Msa0783740-mRNA-1
chr5_2	CDS	67877665	67877693	67877665	ID=Msa0783740-mRNA-1:cds;Parent=Msa0783740-mRNA-1
chr5_2	CDS	67877940	67878117	67877940	ID=Msa0783740-mRNA-1:cds;Parent=Msa0783740-mRNA-1

Gene Sequence	>Msa0783740 ATGGGAAATGCTCTGAGATTCCTCTATGCCAATTGCTGCAAACCAAAAACATCTGATGATCATCAACAATCCACCGTTAGTGTTTCAGCACTTGCTCATGATCTCTTTAACTTTGAAAACACCTCCCAGGTTCCTGAAGGACTAAGCAGACATGTTGTGTATAGAAAGTTAATAGATGGTTGGAAAGAGGCAAGGCCACCACCTAGGACACCTGAAGAAGCAGCTAGACTTGTTATTCTGACATTAGATGGACATAAAAAAGAAGATGTTGAGGTTTTCATGTACTTCCAAACTTCAATTCTACTTAATTTGTTTAAGAGTACTGTCACTGTGTGA
Protein sequence	>Msa0783740 MGNALRFLYANCCKPKTSDDHQQSTVSVSALAHDLFNFENTSQVPEGLSRHVVYRKLIDGWKEARPPPRTPEEAARLVILTLDGHKKEDVEVFMYFQTSILLNLFKSTVTV*