AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0750240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0750240 MtrunA17_Chr1g0169581 71.127 142 40 1 14 154 1 142 4.08e-63 190
Msa0750240 MtrunA17_Chr5g0434951 71.429 140 38 1 14 153 1 138 6.38e-62 187
Msa0750240 MtrunA17_Chr1g0169621 58.434 166 43 1 14 153 1 166 1.16e-55 172
Msa0750240 MtrunA17_Chr3g0111371 62.857 140 47 2 15 154 3 137 1.51e-55 171
Msa0750240 MtrunA17_Chr5g0396261 73.913 115 29 1 41 154 1 115 1.93e-49 154
Msa0750240 MtrunA17_Chr8g0392951 50.365 137 66 1 16 152 23 157 4.25e-36 122
Msa0750240 MtrunA17_Chr6g0482731 46.043 139 72 2 16 153 25 161 1.93e-32 113
Msa0750240 MtrunA17_Chr1g0161211 42.857 133 75 1 20 152 49 180 1.04e-28 104
Msa0750240 MtrunA17_Chr8g0391691 39.850 133 79 1 17 149 36 167 4.15e-26 97.8
Msa0750240 MtrunA17_Chr6g0459231 36.842 133 82 1 20 152 11 141 2.45e-25 94.4
Msa0750240 MtrunA17_Chr7g0256171 40.580 138 79 2 17 152 5 141 2.93e-25 94.4
Msa0750240 MtrunA17_Chr4g0033601 37.410 139 84 2 16 152 4 141 1.05e-24 92.8
Msa0750240 MtrunA17_Chr7g0245911 39.869 153 78 4 11 152 41 190 1.49e-24 94.0
Msa0750240 MtrunA17_Chr8g0351681 45.000 140 68 4 21 152 53 191 5.09e-24 92.4
Msa0750240 MtrunA17_Chr5g0440931 38.235 136 83 1 17 152 12 146 8.05e-23 88.2
Msa0750240 MtrunA17_Chr6g0460211 38.235 136 83 1 17 152 12 146 8.87e-23 87.8
Msa0750240 MtrunA17_Chr7g0254731 37.500 136 84 1 17 152 12 146 1.32e-22 87.4
Msa0750240 MtrunA17_Chr3g0093271 38.235 136 83 1 17 152 63 197 3.38e-22 87.8
Msa0750240 MtrunA17_Chr7g0225921 35.075 134 84 2 20 152 9 140 4.52e-22 85.9
Msa0750240 MtrunA17_Chr1g0166281 36.029 136 86 1 17 152 12 146 2.47e-21 84.3
Msa0750240 MtrunA17_Chr1g0188171 36.641 131 82 1 22 152 17 146 5.08e-21 83.6
Msa0750240 MtrunA17_Chr3g0116151 33.835 133 86 1 20 152 9 139 2.80e-20 81.3
Msa0750240 MtrunA17_Chr3g0116141 33.835 133 86 1 20 152 8 138 3.01e-20 81.3
Msa0750240 MtrunA17_Chr4g0062221 29.286 140 98 1 16 154 10 149 4.28e-20 81.6
Msa0750240 MtrunA17_Chr5g0402331 38.571 140 79 4 16 152 47 182 1.94e-19 80.5
Msa0750240 MtrunA17_Chr4g0044961 37.410 139 84 2 16 154 56 191 2.39e-19 80.5
Msa0750240 MtrunA17_Chr7g0245371 37.762 143 78 3 21 152 28 170 7.59e-19 78.6
Msa0750240 MtrunA17_Chr8g0366401 29.710 138 95 1 17 152 17 154 1.14e-18 77.8
Msa0750240 MtrunA17_Chr5g0402361 38.571 140 79 4 16 152 219 354 2.15e-18 80.1
Msa0750240 MtrunA17_Chr5g0402361 35.036 137 87 1 16 152 47 181 1.76e-16 75.1
Msa0750240 MtrunA17_Chr4g0064231 34.911 169 92 4 1 152 49 216 5.87e-18 77.4
Msa0750240 MtrunA17_Chr1g0185001 32.353 136 90 1 17 152 12 145 3.19e-17 73.6
Msa0750240 MtrunA17_Chr4g0057651 33.088 136 89 1 17 152 6 139 1.16e-16 72.0
Msa0750240 MtrunA17_Chr4g0074811 34.328 134 81 3 21 152 400 528 3.44e-16 74.7
Msa0750240 MtrunA17_Chr4g0057641 32.609 138 91 1 15 152 4 139 4.94e-16 70.5
Msa0750240 MtrunA17_Chr4g0057661 32.609 138 91 1 15 152 4 139 5.04e-16 70.5
Msa0750240 MtrunA17_Chr5g0397101 35.971 139 76 4 21 154 375 505 5.41e-16 73.9
Msa0750240 MtrunA17_Chr8g0373691 33.333 135 85 3 21 153 57 188 6.10e-16 71.2
Msa0750240 MtrunA17_Chr8g0387671 35.252 139 77 4 21 154 375 505 6.37e-16 73.6
Msa0750240 MtrunA17_Chr2g0329371 33.571 140 90 2 16 152 63 202 1.47e-15 70.5
Msa0750240 MtrunA17_Chr5g0444171 35.766 137 75 4 21 152 401 529 2.77e-15 72.0
Msa0750240 MtrunA17_Chr3g0098951 35.507 138 81 2 21 152 392 527 5.03e-15 71.2
Msa0750240 MtrunA17_Chr7g0256701 30.714 140 93 3 17 152 73 212 7.98e-15 68.9
Msa0750240 MtrunA17_Chr8g0368681 28.777 139 98 1 17 154 12 150 9.01e-15 67.4
Msa0750240 MtrunA17_Chr3g0121191 31.655 139 84 2 14 152 90 217 1.21e-14 68.6
Msa0750240 MtrunA17_Chr7g0274101 30.882 136 93 1 17 152 12 146 4.03e-14 65.5
Msa0750240 MtrunA17_Chr6g0450921 29.710 138 96 1 17 154 29 165 1.14e-13 64.7
Msa0750240 MtrunA17_Chr5g0441691 31.387 137 87 3 20 154 376 507 1.95e-13 66.6
Msa0750240 MtrunA17_Chr8g0375741 46.154 65 34 1 17 81 6 69 2.02e-13 62.0
Msa0750240 MtrunA17_Chr5g0434981 51.562 64 30 1 20 83 13 75 2.04e-13 62.0
Msa0750240 MtrunA17_Chr7g0245231 28.676 136 96 1 17 152 30 164 3.78e-13 63.5
Msa0750240 MtrunA17_Chr1g0157131 32.609 138 84 3 20 154 382 513 5.90e-13 65.1
Msa0750240 MtrunA17_Chr1g0160151 32.624 141 80 3 21 152 26 160 3.94e-12 60.8
Msa0750240 MtrunA17_Chr0c02g0489761 30.882 136 87 3 21 154 387 517 7.87e-12 62.0
Msa0750240 MtrunA17_Chr5g0398751 29.851 134 90 3 22 154 17 147 2.17e-11 58.2
Msa0750240 MtrunA17_Chr7g0242561 32.877 146 93 3 10 152 351 494 4.03e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0750240 AT1G18210.2 47.552 143 70 2 11 153 17 154 1.87e-37 126
Msa0750240 AT1G18210.1 47.552 143 70 2 11 153 17 154 1.87e-37 126
Msa0750240 AT5G37770.1 47.222 144 73 2 13 153 13 156 1.05e-34 119
Msa0750240 AT5G37770.2 47.222 144 73 2 13 153 13 156 1.05e-34 119
Msa0750240 AT1G73630.1 42.953 149 80 2 5 153 8 151 3.47e-34 117
Msa0750240 AT1G66400.1 45.390 141 76 1 13 152 11 151 3.98e-33 115
Msa0750240 AT5G17470.1 42.446 139 75 2 18 153 3 139 3.78e-31 109
Msa0750240 AT2G36180.1 43.165 139 73 2 18 152 1 137 4.28e-29 104
Msa0750240 AT1G24620.1 40.876 137 80 1 17 153 37 172 2.94e-27 100
Msa0750240 AT3G07490.1 41.304 138 78 2 17 152 5 141 6.76e-26 96.7
Msa0750240 AT1G05990.1 38.406 138 83 1 17 152 5 142 1.42e-24 92.8
Msa0750240 AT2G15680.1 38.406 138 82 1 16 153 49 183 7.28e-24 92.0
Msa0750240 AT5G21274.1 38.971 136 82 1 17 152 12 146 5.40e-23 89.0
Msa0750240 AT1G66410.1 38.235 136 83 1 17 152 12 146 6.01e-23 89.0
Msa0750240 AT5G37780.1 38.235 136 83 1 17 152 12 146 6.01e-23 89.0
Msa0750240 AT3G43810.1 38.235 136 83 1 17 152 12 146 1.22e-22 87.8
Msa0750240 AT3G56800.1 38.235 136 83 1 17 152 12 146 1.25e-22 87.8
Msa0750240 AT2G41110.1 38.235 136 83 1 17 152 12 146 1.25e-22 87.8
Msa0750240 AT2G27030.1 38.235 136 83 1 17 152 12 146 1.25e-22 87.8
Msa0750240 AT2G27030.3 38.235 136 83 1 17 152 12 146 2.55e-22 88.2
Msa0750240 AT3G43810.2 38.931 131 79 1 22 152 17 146 5.58e-22 87.8
Msa0750240 AT3G03400.1 36.567 134 80 2 15 145 3 134 1.02e-21 85.1
Msa0750240 AT4G03290.1 35.211 142 88 2 17 154 5 146 1.05e-21 85.9
Msa0750240 AT4G12860.1 36.957 138 84 2 17 152 5 141 4.44e-21 84.0
Msa0750240 AT3G10190.1 38.406 138 81 3 17 152 70 205 9.74e-21 84.7
Msa0750240 AT2G43290.1 35.211 142 86 2 17 152 65 206 1.48e-20 84.3
Msa0750240 AT2G41110.2 35.811 148 82 3 17 152 12 158 1.18e-19 80.5
Msa0750240 AT4G37010.1 33.557 149 90 3 12 154 18 163 1.22e-19 80.9
Msa0750240 AT4G37010.2 33.557 149 90 3 12 154 22 167 1.24e-19 80.9
Msa0750240 AT3G22930.1 35.878 131 83 1 22 152 40 169 1.49e-19 80.5
Msa0750240 AT3G50360.1 32.609 138 92 1 17 154 27 163 1.60e-19 80.5
Msa0750240 AT1G32250.1 29.577 142 95 1 16 152 15 156 2.96e-19 79.7
Msa0750240 AT5G37780.2 34.932 146 79 2 22 152 17 161 5.96e-19 79.0
Msa0750240 AT1G66410.4 39.815 108 64 1 45 152 4 110 1.83e-18 76.3
Msa0750240 AT1G66410.3 39.815 108 64 1 45 152 4 110 1.83e-18 76.3
Msa0750240 AT5G37780.4 36.885 122 76 1 31 152 13 133 2.07e-18 76.6
Msa0750240 AT3G59440.1 35.075 134 83 2 21 152 55 186 2.50e-18 78.2
Msa0750240 AT1G66410.2 36.885 122 76 1 31 152 36 156 2.82e-18 77.0
Msa0750240 AT3G43810.4 39.815 108 64 1 45 152 4 110 3.84e-18 75.5
Msa0750240 AT3G43810.3 39.815 108 64 1 45 152 4 110 3.84e-18 75.5
Msa0750240 AT4G14640.1 34.351 131 85 1 22 152 18 147 4.00e-18 76.3
Msa0750240 AT5G37780.3 36.885 122 76 1 31 152 52 172 6.60e-18 76.3
Msa0750240 AT2G41410.1 31.169 154 102 3 2 153 57 208 2.17e-17 75.9
Msa0750240 AT1G18530.1 28.777 139 98 1 17 154 7 145 2.55e-17 74.3
Msa0750240 AT5G42380.1 32.117 137 93 0 16 152 48 184 5.75e-17 74.3
Msa0750240 AT4G04695.1 36.232 138 75 4 20 152 339 468 1.32e-16 76.3
Msa0750240 AT1G76650.1 33.333 138 90 1 15 152 41 176 3.81e-16 72.0
Msa0750240 AT1G76650.3 33.333 138 90 1 15 152 41 176 3.81e-16 72.0
Msa0750240 AT4G20780.1 33.113 151 82 2 21 152 33 183 4.58e-16 72.0
Msa0750240 AT3G50770.1 29.577 142 96 3 16 153 63 204 1.26e-15 71.2
Msa0750240 AT4G04740.4 36.496 137 74 4 21 152 377 505 1.96e-15 72.8
Msa0750240 AT4G04740.6 36.496 137 74 4 21 152 377 505 1.96e-15 72.8
Msa0750240 AT4G04740.5 36.496 137 74 4 21 152 377 505 1.96e-15 72.8
Msa0750240 AT4G04740.3 36.496 137 74 4 21 152 377 505 1.96e-15 72.8
Msa0750240 AT4G04740.1 36.496 137 74 4 21 152 377 505 1.96e-15 72.8
Msa0750240 AT4G04740.7 36.496 137 74 4 21 152 377 505 1.96e-15 72.8
Msa0750240 AT4G23650.1 33.094 139 80 4 21 154 387 517 2.74e-15 72.4
Msa0750240 AT4G04700.1 34.815 135 81 3 20 152 339 468 3.82e-15 72.0
Msa0750240 AT4G21940.1 35.766 137 75 4 21 152 410 538 4.03e-15 72.0
Msa0750240 AT2G41100.4 32.432 148 85 2 22 154 17 164 4.36e-15 71.2
Msa0750240 AT2G41100.4 31.081 148 86 2 22 153 106 253 1.23e-13 67.0
Msa0750240 AT2G41100.1 32.432 148 85 2 22 154 17 164 4.36e-15 71.2
Msa0750240 AT2G41100.1 31.081 148 86 2 22 153 106 253 1.23e-13 67.0
Msa0750240 AT3G03000.1 30.882 136 94 0 17 152 20 155 7.70e-15 68.2
Msa0750240 AT3G03410.1 31.061 132 81 2 21 152 6 127 8.76e-15 67.0
Msa0750240 AT1G76650.2 33.333 138 80 2 15 152 41 166 1.54e-14 67.4
Msa0750240 AT3G25600.1 28.058 139 99 1 17 154 12 150 3.50e-14 66.2
Msa0750240 AT4G14640.2 31.967 122 82 1 31 152 9 129 4.43e-14 65.5
Msa0750240 AT1G61950.1 36.029 136 78 3 20 152 407 536 4.84e-14 68.9
Msa0750240 AT2G41100.7 31.081 148 86 2 22 153 71 218 9.59e-14 67.0
Msa0750240 AT2G41100.6 31.081 148 86 2 22 153 71 218 9.59e-14 67.0
Msa0750240 AT2G41100.3 31.081 148 86 2 22 153 71 218 9.59e-14 67.0
Msa0750240 AT3G20410.2 36.496 137 74 4 21 152 400 528 9.65e-14 67.8
Msa0750240 AT3G20410.1 36.496 137 74 4 21 152 400 528 9.65e-14 67.8
Msa0750240 AT4G04720.2 35.507 138 76 4 20 152 387 516 1.33e-13 67.4
Msa0750240 AT4G04720.1 35.507 138 76 4 20 152 387 516 1.33e-13 67.4
Msa0750240 AT2G41090.1 33.588 131 83 2 22 152 17 143 1.53e-13 65.5
Msa0750240 AT4G21940.2 35.507 138 76 4 20 152 409 538 2.27e-13 67.0
Msa0750240 AT5G17480.1 52.381 63 29 1 21 83 13 74 2.90e-13 62.0
Msa0750240 AT5G19360.1 33.088 136 84 3 21 154 377 507 3.26e-13 66.2
Msa0750240 AT1G50700.1 34.307 137 77 4 21 152 382 510 6.83e-13 65.5
Msa0750240 AT5G12180.1 32.353 136 85 3 21 154 382 512 1.79e-12 64.3
Msa0750240 AT3G01830.1 35.000 140 84 5 16 152 10 145 4.49e-12 60.5
Msa0750240 AT3G03430.1 49.206 63 31 1 21 83 13 74 5.54e-12 58.5
Msa0750240 AT1G76640.1 30.216 139 96 1 15 152 20 158 7.40e-12 60.1
Msa0750240 AT1G74740.1 36.029 136 79 5 20 152 367 497 9.08e-12 62.4
Msa0750240 AT1G62820.1 29.167 144 95 4 15 154 8 148 1.15e-11 59.3
Msa0750240 AT5G04870.1 29.927 137 86 4 21 154 459 588 1.56e-11 61.6
Msa0750240 AT3G51920.1 29.412 136 95 1 17 152 12 146 1.76e-11 58.9
Msa0750240 AT5G44460.1 32.639 144 84 3 21 152 32 174 1.80e-11 59.7
Msa0750240 AT3G51850.1 32.090 134 85 2 21 152 363 492 1.93e-11 61.2
Msa0750240 AT1G76040.2 33.088 136 82 3 22 154 421 550 2.35e-11 60.8
Msa0750240 AT1G76040.1 33.088 136 82 3 22 154 183 312 2.41e-11 60.8
Msa0750240 AT1G76040.4 33.088 136 82 3 22 154 183 312 2.41e-11 60.8
Msa0750240 AT4G04710.2 33.088 136 82 3 20 152 340 469 5.78e-11 59.7
Msa0750240 AT4G04710.1 33.088 136 82 3 20 152 353 482 7.25e-11 59.7
Msa0750240 AT1G21550.1 33.793 145 77 4 21 152 14 152 7.93e-11 57.4
Msa0750240 AT4G04710.3 33.088 136 82 3 20 152 326 455 7.96e-11 59.3

Find 29 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAACAATGTGCAGTTCTTT+TGG 0.251922 5_2:-3653594 Msa0750230:intron
AAGAAATGATTTCGTATTCT+TGG 0.267268 5_2:+3653265 None:intergenic
CACATTGTTCTTAATAAACT+TGG 0.346651 5_2:+3653606 Msa0750240:CDS
TACTTCATCAGATGTCGTTT+TGG 0.347899 5_2:-3653409 Msa0750230:intron
AAAGAAATGCTTTCAATGCT+TGG 0.373424 5_2:+3653384 Msa0750240:CDS
TCATACCGTAATCACCGTTT+TGG 0.378053 5_2:-3653290 Msa0750230:intron
TGGAACCAAAACGGTGATTA+CGG 0.396473 5_2:+3653285 Msa0750240:CDS
AGCATTGAAAGCATTTCTTT+CGG 0.408780 5_2:-3653381 Msa0750230:intron
CGACGTTGCTTATCATTCTC+AGG 0.449084 5_2:-3653653 Msa0750230:intron
TGACGGAAACATTAACTTTG+AGG 0.498713 5_2:+3653686 Msa0750240:CDS
TCGTATTCTTGGAACCAAAA+CGG 0.526744 5_2:+3653276 Msa0750240:CDS
TGAGGAGTTCAAGAAGATGA+TGG 0.534080 5_2:+3653704 Msa0750240:CDS
TTCAACAAGTTCGACAAGAA+CGG 0.554513 5_2:+3653333 Msa0750240:CDS
TGATGAAGTAAAACGCATGA+AGG 0.558820 5_2:+3653422 Msa0750240:CDS
CGGATTGATCAGAACGGCAA+TGG 0.561910 5_2:+3653447 Msa0750240:CDS
CGTCCTTGTCGAGGTCGTAA+AGG 0.575303 5_2:-3653560 Msa0750230:intron
AAGTAAAACGCATGAAGGAA+CGG 0.584140 5_2:+3653427 Msa0750240:CDS
GATTTCCATTGCACTCATGT+TGG 0.588349 5_2:+3653495 Msa0750240:CDS
AAGTTCGACAAGAACGGCGA+CGG 0.611394 5_2:+3653339 Msa0750240:CDS
CGACCTTTACGACCTCGACA+AGG 0.613996 5_2:+3653557 Msa0750240:CDS
CATCACCAACATGAGTGCAA+TGG 0.615226 5_2:-3653500 Msa0750230:intron
AACGTCGATGTTGATGATGA+CGG 0.629007 5_2:+3653669 Msa0750240:CDS
CATTCTCAGGTAGTCACCGA+GGG 0.631930 5_2:-3653640 Msa0750230:intron
CTTGGCGAGAAGTACTCCCT+CGG 0.634170 5_2:+3653624 Msa0750240:CDS
AGATGAGGCCGTCCTTGTCG+AGG 0.636799 5_2:-3653569 Msa0750230:intron
AAGGAACGGATTGATCAGAA+CGG 0.686330 5_2:+3653441 Msa0750240:CDS
CTTTACGACCTCGACAAGGA+CGG 0.703036 5_2:+3653561 Msa0750240:CDS
TCATTCTCAGGTAGTCACCG+AGG 0.703464 5_2:-3653641 Msa0750230:intron
TGATTACGGTATGAACGACG+AGG 0.775749 5_2:+3653299 Msa0750240:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CACATTGTTCTTAATAAACT+TGG + chr5_2:3653606-3653625 Msa0750240:CDS 25.0%
!! AGCATTGAAAGCATTTCTTT+CGG - chr5_2:3653384-3653403 Msa0750230:intron 30.0%
AAAGAAATGCTTTCAATGCT+TGG + chr5_2:3653384-3653403 Msa0750240:CDS 30.0%
TCGTATTCTTGGAACCAAAA+CGG + chr5_2:3653276-3653295 Msa0750240:CDS 35.0%
TTCAACAAGTTCGACAAGAA+CGG + chr5_2:3653333-3653352 Msa0750240:CDS 35.0%
!! TACTTCATCAGATGTCGTTT+TGG - chr5_2:3653412-3653431 Msa0750230:intron 35.0%
TGATGAAGTAAAACGCATGA+AGG + chr5_2:3653422-3653441 Msa0750240:CDS 35.0%
AAGTAAAACGCATGAAGGAA+CGG + chr5_2:3653427-3653446 Msa0750240:CDS 35.0%
! AGAACAATGTGCAGTTCTTT+TGG - chr5_2:3653597-3653616 Msa0750230:intron 35.0%
TGACGGAAACATTAACTTTG+AGG + chr5_2:3653686-3653705 Msa0750240:CDS 35.0%
TGGAACCAAAACGGTGATTA+CGG + chr5_2:3653285-3653304 Msa0750240:CDS 40.0%
! TCATACCGTAATCACCGTTT+TGG - chr5_2:3653293-3653312 Msa0750230:intron 40.0%
!! AAGGAACGGATTGATCAGAA+CGG + chr5_2:3653441-3653460 Msa0750240:CDS 40.0%
GATTTCCATTGCACTCATGT+TGG + chr5_2:3653495-3653514 Msa0750240:CDS 40.0%
!! AACGTCGATGTTGATGATGA+CGG + chr5_2:3653669-3653688 Msa0750240:CDS 40.0%
TGAGGAGTTCAAGAAGATGA+TGG + chr5_2:3653704-3653723 Msa0750240:CDS 40.0%
TGATTACGGTATGAACGACG+AGG + chr5_2:3653299-3653318 Msa0750240:CDS 45.0%
CATCACCAACATGAGTGCAA+TGG - chr5_2:3653503-3653522 Msa0750230:intron 45.0%
!! GCAGTTCTTTTGGAGAGATG+AGG - chr5_2:3653587-3653606 Msa0750230:intron 45.0%
CGACGTTGCTTATCATTCTC+AGG - chr5_2:3653656-3653675 Msa0750230:intron 45.0%
AAGTTCGACAAGAACGGCGA+CGG + chr5_2:3653339-3653358 Msa0750240:CDS 50.0%
CGGATTGATCAGAACGGCAA+TGG + chr5_2:3653447-3653466 Msa0750240:CDS 50.0%
! GTGAAGCTCGTCGTCTTTTC+TGG - chr5_2:3653529-3653548 Msa0750230:intron 50.0%
CTTTACGACCTCGACAAGGA+CGG + chr5_2:3653561-3653580 Msa0750240:CDS 50.0%
CATTCTCAGGTAGTCACCGA+GGG - chr5_2:3653643-3653662 Msa0750230:intron 50.0%
TCATTCTCAGGTAGTCACCG+AGG - chr5_2:3653644-3653663 Msa0750230:intron 50.0%
CGACCTTTACGACCTCGACA+AGG + chr5_2:3653557-3653576 Msa0750240:CDS 55.0%
CGTCCTTGTCGAGGTCGTAA+AGG - chr5_2:3653563-3653582 Msa0750230:intron 55.0%
!! CTTGGCGAGAAGTACTCCCT+CGG + chr5_2:3653624-3653643 Msa0750240:CDS 55.0%
AGATGAGGCCGTCCTTGTCG+AGG - chr5_2:3653572-3653591 Msa0750230:intron 60.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 3653270 3653734 3653270 ID=Msa0750240;Name=Msa0750240
chr5_2 mRNA 3653270 3653734 3653270 ID=Msa0750240-mRNA-1;Parent=Msa0750240;Name=Msa0750240-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|-1|0|1|-1|1|1|0|154
chr5_2 exon 3653270 3653734 3653270 ID=Msa0750240-mRNA-1:exon:1346;Parent=Msa0750240-mRNA-1
chr5_2 CDS 3653270 3653734 3653270 ID=Msa0750240-mRNA-1:cds;Parent=Msa0750240-mRNA-1
Gene Sequence

>Msa0750240

ATGATTTCGTATTCTTGGAACCAAAACGGTGATTACGGTATGAACGACGAGGTGTGTAACATCTTCAACAAGTTCGACAAGAACGGCGACGGAAAAATCTCTCTCTTAGATCCGAAAGAAATGCTTTCAATGCTTGGATCCAAAACGACATCTGATGAAGTAAAACGCATGAAGGAACGGATTGATCAGAACGGCAATGGTTACATTGATCTCAAAGAATTTGCTGATTTCCATTGCACTCATGTTGGTGATGCTGCCAGAAAAGACGACGAGCTTCACGATGCTATCGACCTTTACGACCTCGACAAGGACGGCCTCATCTCTCCAAAAGAACTGCACATTGTTCTTAATAAACTTGGCGAGAAGTACTCCCTCGGTGACTACCTGAGAATGATAAGCAACGTCGATGTTGATGATGACGGAAACATTAACTTTGAGGAGTTCAAGAAGATGATGGCTCGTTGA
Protein sequence

>Msa0750240

MISYSWNQNGDYGMNDEVCNIFNKFDKNGDGKISLLDPKEMLSMLGSKTTSDEVKRMKERIDQNGNGYIDLKEFADFHCTHVGDAARKDDELHDAIDLYDLDKDGLISPKELHIVLNKLGEKYSLGDYLRMISNVDVDDDGNINFEEFKKMMAR*