Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0835190 | XP_003611586.2 | 100.000 | 146 | 0 | 0 | 1 | 146 | 1 | 146 | 2.75e-99 | 291 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0835190 | sp|Q9FJX2|RL262_ARATH | 86.301 | 146 | 20 | 0 | 1 | 146 | 1 | 146 | 2.49e-90 | 261 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0835190 | G7K2J3 | 100.000 | 146 | 0 | 0 | 1 | 146 | 1 | 146 | 1.31e-99 | 291 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0605890 | Msa0835190 | 0.846314 | 2.348523e-59 | -8.615850e-47 |
| Msa0622000 | Msa0835190 | 0.803622 | 3.192887e-49 | -8.615850e-47 |
| Msa0749000 | Msa0835190 | 0.824971 | 6.045128e-54 | -8.615850e-47 |
| Msa0796490 | Msa0835190 | 0.826857 | 2.154456e-54 | -8.615850e-47 |
| Msa0835190 | Msa0978470 | 0.806434 | 8.241575e-50 | -8.615850e-47 |
| Msa0835190 | Msa1169180 | 0.800198 | 1.612442e-48 | -8.615850e-47 |
| Msa0835190 | Msa1208330 | 0.812250 | 4.659272e-51 | -8.615850e-47 |
| Msa0835190 | Msa1311400 | 0.802192 | 6.303469e-49 | -8.615850e-47 |
| Msa0167860 | Msa0835190 | 0.818355 | 2.047059e-52 | -8.615850e-47 |
| Msa0194940 | Msa0835190 | 0.816485 | 5.398930e-52 | -8.615850e-47 |
| Msa0377370 | Msa0835190 | 0.816792 | 4.607179e-52 | -8.615850e-47 |
| Msa0419710 | Msa0835190 | 0.844504 | 7.258681e-59 | -8.615850e-47 |
| Msa0426090 | Msa0835190 | 0.800416 | 1.455898e-48 | -8.615850e-47 |
| Msa0426250 | Msa0835190 | 0.826521 | 2.591772e-54 | -8.615850e-47 |
| Msa0450150 | Msa0835190 | 0.828133 | 1.064757e-54 | -8.615850e-47 |
| Msa0468440 | Msa0835190 | 0.823719 | 1.190596e-53 | -8.615850e-47 |
| Msa0482560 | Msa0835190 | 0.820687 | 6.011922e-53 | -8.615850e-47 |
| Msa0281330 | Msa0835190 | 0.800107 | 1.683383e-48 | -8.615850e-47 |
| Msa0573550 | Msa0835190 | 0.840873 | 6.690188e-58 | -8.615850e-47 |
| Msa0574780 | Msa0835190 | 0.801114 | 1.048885e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0835190 | MtrunA17_Chr5g0401191 | 100.000 | 146 | 0 | 0 | 1 | 146 | 1 | 146 | 2.53e-103 | 291 |
| Msa0835190 | MtrunA17_Chr2g0281331 | 95.205 | 146 | 7 | 0 | 1 | 146 | 1 | 146 | 2.14e-90 | 259 |
| Msa0835190 | MtrunA17_Chr5g0447011 | 91.781 | 146 | 12 | 0 | 1 | 146 | 1 | 146 | 6.44e-88 | 253 |
| Msa0835190 | MtrunA17_Chr8g0370201 | 60.465 | 86 | 7 | 1 | 30 | 115 | 1 | 59 | 7.01e-25 | 90.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0835190 | AT5G67510.1 | 86.301 | 146 | 20 | 0 | 1 | 146 | 1 | 146 | 2.54e-91 | 261 |
| Msa0835190 | AT3G49910.1 | 85.616 | 146 | 21 | 0 | 1 | 146 | 1 | 146 | 2.54e-91 | 261 |
Find 46 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCCCTTCACGTCCCTTGA+AGG | 0.334674 | 5_4:+8481901 | None:intergenic |
| TGCAAGTTTATCGTAGGAAA+TGG | 0.354784 | 5_4:-8481874 | Msa0835190:CDS |
| TTAGTAATCACAACCTTTGA+AGG | 0.371196 | 5_4:+8481785 | None:intergenic |
| TACTAAGATTCGTTTGGATA+AGG | 0.384251 | 5_4:-8481768 | Msa0835190:CDS |
| TGTGATTACTAAGATTCGTT+TGG | 0.392723 | 5_4:-8481774 | Msa0835190:CDS |
| AAAACACGACGGACGCTTGA+TGG | 0.398070 | 5_4:+8482019 | None:intergenic |
| ATTACACGTGAGAAGGTTAA+TGG | 0.405029 | 5_4:-8481833 | Msa0835190:CDS |
| GGGTCGTGCTGCTGCTGATA+AGG | 0.420559 | 5_4:-8481714 | Msa0835190:CDS |
| TCGTAAGGATGATGAAGTTC+AGG | 0.424559 | 5_4:-8481936 | Msa0835190:CDS |
| CGACGGCTGCTTGAAACCCT+AGG | 0.429571 | 5_4:+8482070 | None:intergenic |
| TGCTGCTGCTGATAAGGAAA+AGG | 0.437551 | 5_4:-8481708 | Msa0835190:CDS |
| AAACACGACGGACGCTTGAT+GGG | 0.465655 | 5_4:+8482020 | None:intergenic |
| AATGTCTTCAGGAGCAAACT+TGG | 0.473256 | 5_4:+8481682 | None:intergenic |
| GCGTTCTCTTCCCGTTCGTA+AGG | 0.475960 | 5_4:-8481951 | Msa0835190:CDS |
| GAAAAGCTTGCTTGATCGTA+AGG | 0.501897 | 5_4:-8481741 | Msa0835190:CDS |
| GCAAGTTTATCGTAGGAAAT+GGG | 0.504322 | 5_4:-8481873 | Msa0835190:CDS |
| ACTTCATCATCCTTACGAAC+GGG | 0.506464 | 5_4:+8481941 | None:intergenic |
| TTGATGAGCGCACCTCTCTC+CGG | 0.508962 | 5_4:-8481998 | Msa0835190:CDS |
| ACACAATGAAGTTCAACCCT+AGG | 0.518183 | 5_4:-8482087 | None:intergenic |
| GGTAGTGAGAGGAACCTTCA+AGG | 0.522192 | 5_4:-8481915 | Msa0835190:CDS |
| GTACTTTGAACGGAGGTCAC+CGG | 0.530458 | 5_4:+8481979 | None:intergenic |
| CAGCCGTCGCAAGAGCCGCA+AGG | 0.548428 | 5_4:-8482056 | Msa0835190:CDS |
| CACAATGAAGTTCAACCCTA+GGG | 0.551275 | 5_4:-8482086 | None:intergenic |
| AATGGGTGATTCATATTGAG+AGG | 0.559538 | 5_4:-8481856 | Msa0835190:CDS |
| AACTTCATCATCCTTACGAA+CGG | 0.563073 | 5_4:+8481940 | None:intergenic |
| GGAACCTTCAAGGGACGTGA+AGG | 0.563323 | 5_4:-8481905 | Msa0835190:CDS |
| GCTGCTGCTGATAAGGAAAA+GGG | 0.564379 | 5_4:-8481707 | Msa0835190:CDS |
| GACGGCTGCTTGAAACCCTA+GGG | 0.565492 | 5_4:+8482071 | None:intergenic |
| TGTTGGTGTTGATCCTTCAA+AGG | 0.568879 | 5_4:-8481798 | Msa0835190:CDS |
| GAGCCTTGCGGCTCTTGCGA+CGG | 0.572981 | 5_4:+8482053 | None:intergenic |
| CTTGCTTGATCGTAAGGCGA+AGG | 0.575754 | 5_4:-8481735 | Msa0835190:CDS |
| AACGCACGTTGTACTTTGAA+CGG | 0.578356 | 5_4:+8481969 | None:intergenic |
| AGATTCGTTTGGATAAGGAT+AGG | 0.578817 | 5_4:-8481763 | Msa0835190:CDS |
| AACACGACGGACGCTTGATG+GGG | 0.578903 | 5_4:+8482021 | None:intergenic |
| TGAGAGGATTACACGTGAGA+AGG | 0.580146 | 5_4:-8481840 | Msa0835190:CDS |
| GATGAAGTTCAGGTAGTGAG+AGG | 0.591688 | 5_4:-8481926 | Msa0835190:CDS |
| CTTCAAGGGACGTGAAGGGA+AGG | 0.593141 | 5_4:-8481900 | Msa0835190:CDS |
| AGGTTGTGCAAGTTTATCGT+AGG | 0.600876 | 5_4:-8481880 | Msa0835190:CDS |
| AATGGTCAAACTGTGAATGT+TGG | 0.612366 | 5_4:-8481815 | Msa0835190:CDS |
| TTGCTTGATCGTAAGGCGAA+GGG | 0.621055 | 5_4:-8481734 | Msa0835190:CDS |
| GAACGGAGGTCACCGGAGAG+AGG | 0.625041 | 5_4:+8481986 | None:intergenic |
| GTAGTGAGAGGAACCTTCAA+GGG | 0.634832 | 5_4:-8481914 | Msa0835190:CDS |
| GAACCTTCAAGGGACGTGAA+GGG | 0.643186 | 5_4:-8481904 | Msa0835190:CDS |
| GCACGTTGTACTTTGAACGG+AGG | 0.673729 | 5_4:+8481972 | None:intergenic |
| GGGCGGTGAAATGAGCCTTG+CGG | 0.681827 | 5_4:+8482041 | None:intergenic |
| GTGCGCTCATCAAAACACGA+CGG | 0.772319 | 5_4:+8482008 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTAGTAATCACAACCTTTGA+AGG | + | chr5_4:8481964-8481983 | None:intergenic | 30.0% | |
| TGTGATTACTAAGATTCGTT+TGG | - | chr5_4:8481972-8481991 | Msa0835190:CDS | 30.0% | |
| TACTAAGATTCGTTTGGATA+AGG | - | chr5_4:8481978-8481997 | Msa0835190:CDS | 30.0% | |
| AACTTCATCATCCTTACGAA+CGG | + | chr5_4:8481809-8481828 | None:intergenic | 35.0% | |
| TGCAAGTTTATCGTAGGAAA+TGG | - | chr5_4:8481872-8481891 | Msa0835190:CDS | 35.0% | |
| GCAAGTTTATCGTAGGAAAT+GGG | - | chr5_4:8481873-8481892 | Msa0835190:CDS | 35.0% | |
| AATGGGTGATTCATATTGAG+AGG | - | chr5_4:8481890-8481909 | Msa0835190:CDS | 35.0% | |
| ATTACACGTGAGAAGGTTAA+TGG | - | chr5_4:8481913-8481932 | Msa0835190:CDS | 35.0% | |
| AATGGTCAAACTGTGAATGT+TGG | - | chr5_4:8481931-8481950 | Msa0835190:CDS | 35.0% | |
| AGATTCGTTTGGATAAGGAT+AGG | - | chr5_4:8481983-8482002 | Msa0835190:CDS | 35.0% | |
| ! | ACGTTTTGCATAATGTCTTC+AGG | + | chr5_4:8482078-8482097 | None:intergenic | 35.0% |
| AACGCACGTTGTACTTTGAA+CGG | + | chr5_4:8481780-8481799 | None:intergenic | 40.0% | |
| ACTTCATCATCCTTACGAAC+GGG | + | chr5_4:8481808-8481827 | None:intergenic | 40.0% | |
| TCGTAAGGATGATGAAGTTC+AGG | - | chr5_4:8481810-8481829 | Msa0835190:CDS | 40.0% | |
| AGGTTGTGCAAGTTTATCGT+AGG | - | chr5_4:8481866-8481885 | Msa0835190:CDS | 40.0% | |
| !! | TGTTGGTGTTGATCCTTCAA+AGG | - | chr5_4:8481948-8481967 | Msa0835190:CDS | 40.0% |
| ! | GAAAAGCTTGCTTGATCGTA+AGG | - | chr5_4:8482005-8482024 | Msa0835190:CDS | 40.0% |
| AATGTCTTCAGGAGCAAACT+TGG | + | chr5_4:8482067-8482086 | None:intergenic | 40.0% | |
| GATGAAGTTCAGGTAGTGAG+AGG | - | chr5_4:8481820-8481839 | Msa0835190:CDS | 45.0% | |
| GTAGTGAGAGGAACCTTCAA+GGG | - | chr5_4:8481832-8481851 | Msa0835190:CDS | 45.0% | |
| TGAGAGGATTACACGTGAGA+AGG | - | chr5_4:8481906-8481925 | Msa0835190:CDS | 45.0% | |
| TTGCTTGATCGTAAGGCGAA+GGG | - | chr5_4:8482012-8482031 | Msa0835190:CDS | 45.0% | |
| TGCTGCTGCTGATAAGGAAA+AGG | - | chr5_4:8482038-8482057 | Msa0835190:CDS | 45.0% | |
| GCTGCTGCTGATAAGGAAAA+GGG | - | chr5_4:8482039-8482058 | Msa0835190:CDS | 45.0% | |
| AAACACGACGGACGCTTGAT+GGG | + | chr5_4:8481729-8481748 | None:intergenic | 50.0% | |
| AAAACACGACGGACGCTTGA+TGG | + | chr5_4:8481730-8481749 | None:intergenic | 50.0% | |
| GTGCGCTCATCAAAACACGA+CGG | + | chr5_4:8481741-8481760 | None:intergenic | 50.0% | |
| GTACTTTGAACGGAGGTCAC+CGG | + | chr5_4:8481770-8481789 | None:intergenic | 50.0% | |
| GCACGTTGTACTTTGAACGG+AGG | + | chr5_4:8481777-8481796 | None:intergenic | 50.0% | |
| GGTAGTGAGAGGAACCTTCA+AGG | - | chr5_4:8481831-8481850 | Msa0835190:CDS | 50.0% | |
| GAACCTTCAAGGGACGTGAA+GGG | - | chr5_4:8481842-8481861 | Msa0835190:CDS | 50.0% | |
| CTTGCTTGATCGTAAGGCGA+AGG | - | chr5_4:8482011-8482030 | Msa0835190:CDS | 50.0% | |
| GACGGCTGCTTGAAACCCTA+GGG | + | chr5_4:8481678-8481697 | None:intergenic | 55.0% | |
| AACACGACGGACGCTTGATG+GGG | + | chr5_4:8481728-8481747 | None:intergenic | 55.0% | |
| TTGATGAGCGCACCTCTCTC+CGG | - | chr5_4:8481748-8481767 | Msa0835190:CDS | 55.0% | |
| GCGTTCTCTTCCCGTTCGTA+AGG | - | chr5_4:8481795-8481814 | Msa0835190:CDS | 55.0% | |
| GGAACCTTCAAGGGACGTGA+AGG | - | chr5_4:8481841-8481860 | Msa0835190:CDS | 55.0% | |
| CTTCCCTTCACGTCCCTTGA+AGG | + | chr5_4:8481848-8481867 | None:intergenic | 55.0% | |
| CTTCAAGGGACGTGAAGGGA+AGG | - | chr5_4:8481846-8481865 | Msa0835190:CDS | 55.0% | |
| CGACGGCTGCTTGAAACCCT+AGG | + | chr5_4:8481679-8481698 | None:intergenic | 60.0% | |
| GGGCGGTGAAATGAGCCTTG+CGG | + | chr5_4:8481708-8481727 | None:intergenic | 60.0% | |
| ! | GGGTCGTGCTGCTGCTGATA+AGG | - | chr5_4:8482032-8482051 | Msa0835190:CDS | 60.0% |
| GAGCCTTGCGGCTCTTGCGA+CGG | + | chr5_4:8481696-8481715 | None:intergenic | 65.0% | |
| ACGACGGACGCTTGATGGGG+CGG | + | chr5_4:8481725-8481744 | None:intergenic | 65.0% | |
| GAACGGAGGTCACCGGAGAG+AGG | + | chr5_4:8481763-8481782 | None:intergenic | 65.0% | |
| CAGCCGTCGCAAGAGCCGCA+AGG | - | chr5_4:8481690-8481709 | Msa0835190:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_4 | gene | 8481664 | 8482104 | 8481664 | ID=Msa0835190;Name=Msa0835190 |
| chr5_4 | mRNA | 8481664 | 8482104 | 8481664 | ID=Msa0835190-mRNA-1;Parent=Msa0835190;Name=Msa0835190-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|146 |
| chr5_4 | exon | 8481664 | 8482104 | 8481664 | ID=Msa0835190-mRNA-1:exon:3739;Parent=Msa0835190-mRNA-1 |
| chr5_4 | CDS | 8481664 | 8482104 | 8481664 | ID=Msa0835190-mRNA-1:cds;Parent=Msa0835190-mRNA-1 |
| Gene Sequence |
| Protein sequence |
