AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1084120

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1084120 MtrunA17_Chr7g0271671 86.735 98 13 0 1 98 1 98 1.57e-53 175
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 126 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTATCACTGGTTGGTCTATT+TGG 0.168608 7_2:+84403556 Msa1084120:three_prime_UTR
GAGATAGACGGCCACATTTC+TGG 0.180333 7_2:-84403442 None:intergenic
TGATGTTTGCACAACAAAAT+TGG 0.211587 7_2:-84403659 None:intergenic
CGAACATTCAGGACCTTAAA+AGG 0.238490 7_2:-84402267 None:intergenic
GGACATGTGAAAATTGTTTC+AGG 0.259512 7_2:-84401884 None:intergenic
TTCTTACTTCTTAGTCTATA+AGG 0.280469 7_2:-84403963 None:intergenic
CACCATACAGGACACATAAT+TGG 0.280822 7_2:-84402004 None:intergenic
CCATCCAACTGCTAGCTAAC+TGG 0.287391 7_2:-84404019 None:intergenic
AGCTAGCAGTTGGATGGCCT+GGG 0.307641 7_2:+84404025 Msa1084120:three_prime_UTR
GATGTTTGCACAACAAAATT+GGG 0.319284 7_2:-84403658 None:intergenic
TTGGAAAGTCAAACTGTTGC+AGG 0.322001 7_2:-84403195 None:intergenic
TACCCTTTCCTCGAACATTC+AGG 0.328468 7_2:-84402278 None:intergenic
ATCTTAGTGCTTCTTGTGTT+TGG 0.331751 7_2:+84400896 Msa1084120:five_prime_UTR
ATCTCCCTCAGCTTTGATTT+CGG 0.333180 7_2:-84401905 None:intergenic
AAAACACGTACGGAAATAAA+AGG 0.334232 7_2:-84401114 None:intergenic
TAGCTAGCAGTTGGATGGCC+TGG 0.341532 7_2:+84404024 Msa1084120:three_prime_UTR
AGAGAAGAACCGGTCAATTT+CGG 0.341595 7_2:+84402182 Msa1084120:three_prime_UTR
TGTGATCAAATGGCAGCATT+AGG 0.348917 7_2:+84403499 Msa1084120:three_prime_UTR
TTAGTTCAATCTAATTCAAT+TGG 0.354677 7_2:+84402216 Msa1084120:three_prime_UTR
CCATGTATTGTTGTATCAAT+TGG 0.354774 7_2:-84403115 None:intergenic
ATTCTCTTAGTACTAGTAGA+AGG 0.362152 7_2:-84401049 None:intergenic
CATTATTGAATGATAAAGTA+TGG 0.373290 7_2:+84402419 Msa1084120:three_prime_UTR
AGTTGTCCATTTCCAACTCT+TGG 0.375977 7_2:-84402080 None:intergenic
ATGTGTTGGCCAACTCGTTA+AGG 0.382699 7_2:-84401818 None:intergenic
TTAAAGACAAGGGAGGATAT+TGG 0.397067 7_2:-84402788 None:intergenic
CATACTTTATCATTCAATAA+TGG 0.400855 7_2:-84402418 None:intergenic
ACCTCTTCTCCAGAAAAGTA+TGG 0.401455 7_2:-84403535 None:intergenic
GCAAAACGCAAGATTGTTAC+TGG 0.404835 7_2:-84401256 None:intergenic
GTCCTGAATGTTCGAGGAAA+GGG 0.408908 7_2:+84402276 Msa1084120:three_prime_UTR
ATTCAATTGGAGCAAGCATT+CGG 0.413576 7_2:+84402229 Msa1084120:three_prime_UTR
AAAATCATTACACTAAGAAA+TGG 0.418028 7_2:+84402116 Msa1084120:three_prime_UTR
AGAGTACCTTGCTGAGCAAT+TGG 0.420158 7_2:-84403214 None:intergenic
TTCAATTGGAGCAAGCATTC+GGG 0.420187 7_2:+84402230 Msa1084120:three_prime_UTR
ATCTCACAAAGTGGACTTGT+AGG 0.422654 7_2:+84403466 Msa1084120:three_prime_UTR
ATTTCTGGTTGGCATGGAAA+TGG 0.424406 7_2:-84403427 None:intergenic
ACAAGACTGGAGGGAAGTTA+TGG 0.438740 7_2:+84402690 Msa1084120:three_prime_UTR
TTGTAGAATAAGCACTCAAC+AGG 0.441707 7_2:-84403393 None:intergenic
CGAACGTTTACTTCGAAAAG+TGG 0.444298 7_2:+84401281 Msa1084120:CDS
AATAGAAATACCTTGAGGTT+AGG 0.444750 7_2:-84401459 None:intergenic
ATCTCTCAATCCACATGAAA+AGG 0.445518 7_2:+84402871 Msa1084120:three_prime_UTR
GATATATCAAAGAGAAGAAC+CGG 0.455709 7_2:+84402172 Msa1084120:three_prime_UTR
TTCTGGAGAAGAGGTATCAC+TGG 0.456301 7_2:+84403543 Msa1084120:three_prime_UTR
CTGTCATTGGCAGTGCTTGG+TGG 0.456561 7_2:+84403042 Msa1084120:intron
TTTAAGGTCCTGAATGTTCG+AGG 0.478176 7_2:+84402270 Msa1084120:three_prime_UTR
AGATGGAATACACACTGTTA+AGG 0.480073 7_2:+84402492 Msa1084120:three_prime_UTR
TGTGTTGGCCAACTCGTTAA+GGG 0.482570 7_2:-84401817 None:intergenic
TTTGTTTATGATGACGGAGC+AGG 0.488123 7_2:+84403613 Msa1084120:three_prime_UTR
CATTGTGATGTAGATGATGA+TGG 0.491743 7_2:+84402737 Msa1084120:three_prime_UTR
GTACCTCCAAGAGTTGGAAA+TGG 0.493683 7_2:+84402074 Msa1084120:three_prime_UTR
TGATACAACAATACATGGTA+TGG 0.502118 7_2:+84403120 Msa1084120:three_prime_UTR
AGTGTACAAACTGCACAATA+TGG 0.502488 7_2:+84403157 Msa1084120:three_prime_UTR
TGTCCATTTCCAACTCTTGG+AGG 0.507896 7_2:-84402077 None:intergenic
CATCCAACTGCTAGCTAACT+GGG 0.509982 7_2:-84404018 None:intergenic
AGGTAGTGGTGGAGCTTATG+TGG 0.510631 7_2:-84401439 None:intergenic
AGATTCGATCTACTCTCAAA+TGG 0.511809 7_2:+84403713 Msa1084120:three_prime_UTR
TAACGAAGATTCATTAAGAA+AGG 0.512049 7_2:+84402537 Msa1084120:three_prime_UTR
TAACCCTAGCCGAAATTGAC+CGG 0.513525 7_2:-84402191 None:intergenic
CCAATTGATACAACAATACA+TGG 0.513913 7_2:+84403115 Msa1084120:three_prime_UTR
GAACCGGTCAATTTCGGCTA+GGG 0.516011 7_2:+84402188 Msa1084120:three_prime_UTR
AAATTGAATGGCATCGAAGA+GGG 0.521226 7_2:+84400979 Msa1084120:exon
GGCCACATTTCTGGTTGGCA+TGG 0.528202 7_2:-84403433 None:intergenic
AGTCTTATATTTCAATCTAG+AGG 0.534004 7_2:-84403928 None:intergenic
GCAAATGACAAAGCAGCCCC+AGG 0.535908 7_2:-84404042 None:intergenic
AAATCAGCAACGCGTAGTCA+TGG 0.540990 7_2:+84401959 Msa1084120:three_prime_UTR
CACAGGCAGTTGACATCAGA+TGG 0.542027 7_2:+84402475 Msa1084120:three_prime_UTR
TGATAAAGTATGGCGTCTGA+AGG 0.545484 7_2:+84402429 Msa1084120:three_prime_UTR
AAATAGAAAATAGACAAGAC+TGG 0.545967 7_2:+84402677 Msa1084120:intron
TGTTGTATGTAGCTAGAAAG+AGG 0.546402 7_2:+84403740 Msa1084120:three_prime_UTR
ATGTCCTTTGTTTATGATGA+CGG 0.547991 7_2:+84403607 Msa1084120:three_prime_UTR
GATAAAGTATGGCGTCTGAA+GGG 0.551278 7_2:+84402430 Msa1084120:three_prime_UTR
CAAATTGTACCTCCAAGAGT+TGG 0.551489 7_2:+84402068 Msa1084120:three_prime_UTR
ATCGAAGAGGGCATCTCATG+TGG 0.556518 7_2:+84400991 Msa1084120:CDS
GTAAAGATGGTACACTTCAC+AGG 0.556690 7_2:+84402458 Msa1084120:three_prime_UTR
TCACGCAGTTCAATTTGAAG+TGG 0.561167 7_2:-84401931 None:intergenic
ATCCAATTATGTGTCCTGTA+TGG 0.561327 7_2:+84402002 Msa1084120:three_prime_UTR
ATACCCAGTTAGCTAGCAGT+TGG 0.564347 7_2:+84404015 Msa1084120:three_prime_UTR
GTCTCTCCTCTCATATGTGT+TGG 0.564755 7_2:-84401832 None:intergenic
TGGATTGACCCTTAACGAGT+TGG 0.565769 7_2:+84401809 Msa1084120:CDS
AGAACCGGTCAATTTCGGCT+AGG 0.566130 7_2:+84402187 Msa1084120:three_prime_UTR
ACCTTGAGGTTAGGTAGTGG+TGG 0.567120 7_2:-84401450 None:intergenic
GTTCCTTAACTAGTTGCAAG+AGG 0.567391 7_2:+84401411 Msa1084120:intron
ATGTTTGCACAACAAAATTG+GGG 0.568691 7_2:-84403657 None:intergenic
TAAAATTGAATTAAAGACAA+GGG 0.568925 7_2:-84402798 None:intergenic
CTGGTTGGCATGGAAATGGT+AGG 0.572443 7_2:-84403423 None:intergenic
CGTACGGAAATAAAAGGTGT+TGG 0.572739 7_2:-84401108 None:intergenic
GGTCCTGAATGTTCGAGGAA+AGG 0.575080 7_2:+84402275 Msa1084120:three_prime_UTR
AGAAAATAGACAAGACTGGA+GGG 0.587832 7_2:+84402681 Msa1084120:intron
GGAGAAGAGGTATCACTGGT+TGG 0.588993 7_2:+84403547 Msa1084120:three_prime_UTR
CTGAAGGGGATACGTAAAGA+TGG 0.592154 7_2:+84402445 Msa1084120:three_prime_UTR
CAACGCGTAGTCATGGAAGA+AGG 0.592392 7_2:+84401966 Msa1084120:three_prime_UTR
TCTATCTCAATCTCACAAAG+TGG 0.592542 7_2:+84403457 Msa1084120:three_prime_UTR
GACTTTCCAATTGCTCAGCA+AGG 0.595283 7_2:+84403208 Msa1084120:three_prime_UTR
TGGAAAGTCAAACTGTTGCA+GGG 0.596726 7_2:-84403194 None:intergenic
TAGACGGCCACATTTCTGGT+TGG 0.599588 7_2:-84403438 None:intergenic
ATAAAGTATGGCGTCTGAAG+GGG 0.600694 7_2:+84402431 Msa1084120:three_prime_UTR
CCAGTTAGCTAGCAGTTGGA+TGG 0.602429 7_2:+84404019 Msa1084120:three_prime_UTR
TTCCATGCCAACCAGAAATG+TGG 0.602938 7_2:+84403431 Msa1084120:three_prime_UTR
CTTTATCATTCAATAATGGA+CGG 0.610044 7_2:-84402414 None:intergenic
AATTGAATTAAAGACAAGGG+AGG 0.612137 7_2:-84402795 None:intergenic
TCCACCACTACCTAACCTCA+AGG 0.613550 7_2:+84401449 Msa1084120:CDS
GTTGTATGTAGCTAGAAAGA+GGG 0.617369 7_2:+84403741 Msa1084120:three_prime_UTR
ATGTCCGAAATCAAAGCTGA+GGG 0.617513 7_2:+84401901 Msa1084120:three_prime_UTR
GGAAATGGACAACTACTGAA+AGG 0.620282 7_2:+84402089 Msa1084120:three_prime_UTR
AATACCTTGAGGTTAGGTAG+TGG 0.621059 7_2:-84401453 None:intergenic
CATGTCCGAAATCAAAGCTG+AGG 0.623360 7_2:+84401900 Msa1084120:three_prime_UTR
CTTTGTGAGATTGAGATAGA+CGG 0.623813 7_2:-84403454 None:intergenic
ATTGTTGTATCAATTGGCAA+TGG 0.633369 7_2:-84403109 None:intergenic
TAGTTCTAGTAGTGATGACA+AGG 0.633706 7_2:+84401015 Msa1084120:CDS
GCTAGCAGTTGGATGGCCTG+GGG 0.636173 7_2:+84404026 Msa1084120:three_prime_UTR
TTAAAATTGAATTAAAGACA+AGG 0.637130 7_2:-84402799 None:intergenic
AAAATTGAATGGCATCGAAG+AGG 0.644164 7_2:+84400978 Msa1084120:exon
TACAACAATACATGGTATGG+TGG 0.645400 7_2:+84403123 Msa1084120:three_prime_UTR
AAAAGAGGATTGGACAGCTG+AGG 0.645437 7_2:+84402034 Msa1084120:three_prime_UTR
TAGAAAATAGACAAGACTGG+AGG 0.645484 7_2:+84402680 Msa1084120:intron
ATTGTGATGTAGATGATGAT+GGG 0.651673 7_2:+84402738 Msa1084120:three_prime_UTR
TGCTCCGTCATCATAAACAA+AGG 0.659879 7_2:-84403611 None:intergenic
TCGAAGAGGGCATCTCATGT+GGG 0.661983 7_2:+84400992 Msa1084120:CDS
GTACTAAGAGAATAAAACAT+GGG 0.665045 7_2:+84401059 Msa1084120:CDS
TCTATTTGGAAGTTACACGT+TGG 0.667355 7_2:+84403570 Msa1084120:three_prime_UTR
ATTGGAGCAAGCATTCGGGA+AGG 0.671894 7_2:+84402234 Msa1084120:three_prime_UTR
AGTACTAAGAGAATAAAACA+TGG 0.690543 7_2:+84401058 Msa1084120:CDS
AGTTGGCCAACACATATGAG+AGG 0.691689 7_2:+84401826 Msa1084120:CDS
TGTTTGCACAACAAAATTGG+GGG 0.694097 7_2:-84403656 None:intergenic
AAACGCAAGATTGTTACTGG+CGG 0.706358 7_2:-84401253 None:intergenic
GTACAAACTGCACAATATGG+TGG 0.713505 7_2:+84403160 Msa1084120:three_prime_UTR
ACATAATTGGATCTTCATCG+AGG 0.760970 7_2:-84401991 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATAAATACATTTACTTTT+TGG + chr7_2:84400931-84400950 Msa1084120:five_prime_UTR 10.0%
!!! CATTCAATTTTTTTTTTTTT+TGG - chr7_2:84400969-84400988 None:intergenic 10.0%
!! CAAAAAAAAAAAAAATTGAA+TGG + chr7_2:84400967-84400986 Msa1084120:exon 10.0%
!! ATTAAATCAAAATTCTTTAA+AGG - chr7_2:84401605-84401624 None:intergenic 10.0%
!!! TTTTACAAAATTTATTTTTG+TGG - chr7_2:84401653-84401672 None:intergenic 10.0%
!!! ATACTATTTCATATAATTTT+TGG + chr7_2:84402647-84402666 Msa1084120:intron 10.0%
!!! ATATATACACAATTATATTT+TGG - chr7_2:84402983-84403002 None:intergenic 10.0%
!! AAAATAAAATAAAACACGTA+CGG - chr7_2:84401127-84401146 None:intergenic 15.0%
!!! ATTTTTTTACATTTTGTTAG+AGG - chr7_2:84402313-84402332 None:intergenic 15.0%
!! AAAATGTAAAAAAATGTACA+TGG + chr7_2:84402318-84402337 Msa1084120:intron 15.0%
!! AAATGTAAAAAAATGTACAT+GGG + chr7_2:84402319-84402338 Msa1084120:intron 15.0%
!! TAAAATTGAATTAAAGACAA+GGG - chr7_2:84402801-84402820 None:intergenic 15.0%
!! TTAAAATTGAATTAAAGACA+AGG - chr7_2:84402802-84402821 None:intergenic 15.0%
!!! TGTCTTTAATTCAATTTTAA+AGG + chr7_2:84402802-84402821 Msa1084120:three_prime_UTR 15.0%
!! AATTGAAACTGAAAAAATAT+TGG + chr7_2:84403686-84403705 Msa1084120:three_prime_UTR 15.0%
!!! ACAATTTTCAATTTTTTACA+CGG - chr7_2:84404100-84404119 None:intergenic 15.0%
!!! TTTTTTTTTGGTTGTTGAAA+AGG - chr7_2:84400957-84400976 None:intergenic 20.0%
!! AAAAAAATAGAAATACCTTG+AGG - chr7_2:84401467-84401486 None:intergenic 20.0%
!! TTAGTTGTTAAAAATGTAGA+TGG + chr7_2:84401504-84401523 Msa1084120:intron 20.0%
!!! GAATTTTGATTTAATTTGAG+TGG + chr7_2:84401610-84401629 Msa1084120:intron 20.0%
!!! TACAAAATTTATTTTTGTGG+TGG - chr7_2:84401650-84401669 None:intergenic 20.0%
!! AAAATCATTACACTAAGAAA+TGG + chr7_2:84402116-84402135 Msa1084120:three_prime_UTR 20.0%
!! TTAGTTCAATCTAATTCAAT+TGG + chr7_2:84402216-84402235 Msa1084120:three_prime_UTR 20.0%
!! CATACTTTATCATTCAATAA+TGG - chr7_2:84402421-84402440 None:intergenic 20.0%
!! CATTATTGAATGATAAAGTA+TGG + chr7_2:84402419-84402438 Msa1084120:three_prime_UTR 20.0%
!!! TTTCATATAATTTTTGGCTT+TGG + chr7_2:84402653-84402672 Msa1084120:intron 20.0%
!!! TAATTCAATTTTAAAGGTTG+TGG + chr7_2:84402808-84402827 Msa1084120:three_prime_UTR 20.0%
! AGTACTAAGAGAATAAAACA+TGG + chr7_2:84401058-84401077 Msa1084120:CDS 25.0%
! GTACTAAGAGAATAAAACAT+GGG + chr7_2:84401059-84401078 Msa1084120:CDS 25.0%
!! AAAGTAAGAGTATGAATGAT+TGG - chr7_2:84401315-84401334 None:intergenic 25.0%
!! TTACATTTTGTTAGAGGTTT+AGG - chr7_2:84402307-84402326 None:intergenic 25.0%
!! CTTTATCATTCAATAATGGA+CGG - chr7_2:84402417-84402436 None:intergenic 25.0%
! TAACGAAGATTCATTAAGAA+AGG + chr7_2:84402537-84402556 Msa1084120:three_prime_UTR 25.0%
! AAATAGAAAATAGACAAGAC+TGG + chr7_2:84402677-84402696 Msa1084120:intron 25.0%
!! TATTTGGAGTGAACTTTATA+TGG + chr7_2:84402928-84402947 Msa1084120:intron 25.0%
!! ATATTCTGCATAAACACTTT+TGG + chr7_2:84403245-84403264 Msa1084120:intron 25.0%
! ATATCCTAATTCTATCATTC+AGG + chr7_2:84403370-84403389 Msa1084120:intron 25.0%
!! TTCTGAATTTTGTGATCAAA+TGG + chr7_2:84403489-84403508 Msa1084120:three_prime_UTR 25.0%
! TTTGCTTACTCAAAAAATAC+TGG + chr7_2:84403815-84403834 Msa1084120:three_prime_UTR 25.0%
!! AGTCTTATATTTCAATCTAG+AGG - chr7_2:84403931-84403950 None:intergenic 25.0%
! TTCTTACTTCTTAGTCTATA+AGG - chr7_2:84403966-84403985 None:intergenic 25.0%
ATTCTCTTAGTACTAGTAGA+AGG - chr7_2:84401052-84401071 None:intergenic 30.0%
!!! ATCTCTTTTTGTTGTTCTTC+TGG - chr7_2:84401088-84401107 None:intergenic 30.0%
AAAACACGTACGGAAATAAA+AGG - chr7_2:84401117-84401136 None:intergenic 30.0%
AATAAGAAAGGAACATGTAG+AGG - chr7_2:84401196-84401215 None:intergenic 30.0%
GAAGTCAAGTCAAATAAGAA+AGG - chr7_2:84401208-84401227 None:intergenic 30.0%
TGCTTCATGGATTCTAATAA+TGG + chr7_2:84401350-84401369 Msa1084120:intron 30.0%
CAGTTAATAGCATCAAAGAT+TGG - chr7_2:84401379-84401398 None:intergenic 30.0%
CAATCTTTGATGCTATTAAC+TGG + chr7_2:84401377-84401396 Msa1084120:intron 30.0%
AATAGAAATACCTTGAGGTT+AGG - chr7_2:84401462-84401481 None:intergenic 30.0%
!! TTTAATTTGAGTGGTGAAAG+TGG + chr7_2:84401619-84401638 Msa1084120:intron 30.0%
! ATAGCCAATTTAAGACCTAA+AGG + chr7_2:84401696-84401715 Msa1084120:intron 30.0%
TTTGCCTTTAGGTCTTAAAT+TGG - chr7_2:84401703-84401722 None:intergenic 30.0%
! AAGTCTTGATTTTTGCCTTT+AGG - chr7_2:84401714-84401733 None:intergenic 30.0%
! TTGTGTTTCTAATTTAGCTG+TGG + chr7_2:84401789-84401808 Msa1084120:intron 30.0%
GATATATCAAAGAGAAGAAC+CGG + chr7_2:84402172-84402191 Msa1084120:three_prime_UTR 30.0%
AGCTCTTGAAGTTATTAGTT+TGG + chr7_2:84402617-84402636 Msa1084120:intron 30.0%
ATTGTGATGTAGATGATGAT+GGG + chr7_2:84402738-84402757 Msa1084120:three_prime_UTR 30.0%
AATTGAATTAAAGACAAGGG+AGG - chr7_2:84402798-84402817 None:intergenic 30.0%
!! TTCAATTTTAAAGGTTGTGG+AGG + chr7_2:84402811-84402830 Msa1084120:three_prime_UTR 30.0%
TACTTGTATCCTACTTTCTT+TGG + chr7_2:84402898-84402917 Msa1084120:intron 30.0%
TTTCTTTGGCTGTGAATATT+TGG + chr7_2:84402912-84402931 Msa1084120:intron 30.0%
TTTGATCATATCAGGTTAAG+AGG - chr7_2:84403016-84403035 None:intergenic 30.0%
!!! AGAAGTGTTTTGATCATATC+AGG - chr7_2:84403024-84403043 None:intergenic 30.0%
ATTGTTGTATCAATTGGCAA+TGG - chr7_2:84403112-84403131 None:intergenic 30.0%
CCATGTATTGTTGTATCAAT+TGG - chr7_2:84403118-84403137 None:intergenic 30.0%
CCAATTGATACAACAATACA+TGG + chr7_2:84403115-84403134 Msa1084120:three_prime_UTR 30.0%
TGATACAACAATACATGGTA+TGG + chr7_2:84403120-84403139 Msa1084120:three_prime_UTR 30.0%
! ATGTCCTTTGTTTATGATGA+CGG + chr7_2:84403607-84403626 Msa1084120:three_prime_UTR 30.0%
ATGTTTGCACAACAAAATTG+GGG - chr7_2:84403660-84403679 None:intergenic 30.0%
GATGTTTGCACAACAAAATT+GGG - chr7_2:84403661-84403680 None:intergenic 30.0%
TGATGTTTGCACAACAAAAT+TGG - chr7_2:84403662-84403681 None:intergenic 30.0%
! AAGCAAAAAGCATGAACTAA+GGG - chr7_2:84403802-84403821 None:intergenic 30.0%
! TAAGCAAAAAGCATGAACTA+AGG - chr7_2:84403803-84403822 None:intergenic 30.0%
ATCTTAGTGCTTCTTGTGTT+TGG + chr7_2:84400896-84400915 Msa1084120:five_prime_UTR 35.0%
! AAAATTGAATGGCATCGAAG+AGG + chr7_2:84400978-84400997 Msa1084120:exon 35.0%
! AAATTGAATGGCATCGAAGA+GGG + chr7_2:84400979-84400998 Msa1084120:exon 35.0%
!! TAGTTCTAGTAGTGATGACA+AGG + chr7_2:84401015-84401034 Msa1084120:CDS 35.0%
GGACATGTGAAAATTGTTTC+AGG - chr7_2:84401887-84401906 None:intergenic 35.0%
ACATAATTGGATCTTCATCG+AGG - chr7_2:84401994-84402013 None:intergenic 35.0%
ATCCAATTATGTGTCCTGTA+TGG + chr7_2:84402002-84402021 Msa1084120:three_prime_UTR 35.0%
!!! CTTTTTCAAAGTCACCATAC+AGG - chr7_2:84402019-84402038 None:intergenic 35.0%
! GTATGGTGACTTTGAAAAAG+AGG + chr7_2:84402019-84402038 Msa1084120:three_prime_UTR 35.0%
! GTGACTTTGAAAAAGAGGAT+TGG + chr7_2:84402024-84402043 Msa1084120:three_prime_UTR 35.0%
ATTCAATTGGAGCAAGCATT+CGG + chr7_2:84402229-84402248 Msa1084120:three_prime_UTR 35.0%
AGATGGAATACACACTGTTA+AGG + chr7_2:84402492-84402511 Msa1084120:three_prime_UTR 35.0%
TAGAAAATAGACAAGACTGG+AGG + chr7_2:84402680-84402699 Msa1084120:intron 35.0%
AGAAAATAGACAAGACTGGA+GGG + chr7_2:84402681-84402700 Msa1084120:intron 35.0%
CATTGTGATGTAGATGATGA+TGG + chr7_2:84402737-84402756 Msa1084120:three_prime_UTR 35.0%
TTAAAGACAAGGGAGGATAT+TGG - chr7_2:84402791-84402810 None:intergenic 35.0%
ATCTCTCAATCCACATGAAA+AGG + chr7_2:84402871-84402890 Msa1084120:three_prime_UTR 35.0%
! CAAGTACAAACCTTTTCATG+TGG - chr7_2:84402884-84402903 None:intergenic 35.0%
TATTCACAGCCAAAGAAAGT+AGG - chr7_2:84402910-84402929 None:intergenic 35.0%
GATCAAAACACTTCTGTCAT+TGG + chr7_2:84403029-84403048 Msa1084120:intron 35.0%
TACAACAATACATGGTATGG+TGG + chr7_2:84403123-84403142 Msa1084120:three_prime_UTR 35.0%
AGTGTACAAACTGCACAATA+TGG + chr7_2:84403157-84403176 Msa1084120:three_prime_UTR 35.0%
TGAAAAAGTCTGAGTTGTCT+AGG + chr7_2:84403282-84403301 Msa1084120:intron 35.0%
TTGTAGAATAAGCACTCAAC+AGG - chr7_2:84403396-84403415 None:intergenic 35.0%
CTTTGTGAGATTGAGATAGA+CGG - chr7_2:84403457-84403476 None:intergenic 35.0%
TCTATCTCAATCTCACAAAG+TGG + chr7_2:84403457-84403476 Msa1084120:three_prime_UTR 35.0%
TCTATTTGGAAGTTACACGT+TGG + chr7_2:84403570-84403589 Msa1084120:three_prime_UTR 35.0%
TGTTTGCACAACAAAATTGG+GGG - chr7_2:84403659-84403678 None:intergenic 35.0%
AGATTCGATCTACTCTCAAA+TGG + chr7_2:84403713-84403732 Msa1084120:three_prime_UTR 35.0%
TGTTGTATGTAGCTAGAAAG+AGG + chr7_2:84403740-84403759 Msa1084120:three_prime_UTR 35.0%
GTTGTATGTAGCTAGAAAGA+GGG + chr7_2:84403741-84403760 Msa1084120:three_prime_UTR 35.0%
!! GCTTCATACTTTTTGCTTAC+AGG - chr7_2:84403877-84403896 None:intergenic 35.0%
! CGTACGGAAATAAAAGGTGT+TGG - chr7_2:84401111-84401130 None:intergenic 40.0%
TACATGCAGATGCAGAACAT+TGG - chr7_2:84401173-84401192 None:intergenic 40.0%
AAACGCAAGATTGTTACTGG+CGG - chr7_2:84401256-84401275 None:intergenic 40.0%
GCAAAACGCAAGATTGTTAC+TGG - chr7_2:84401259-84401278 None:intergenic 40.0%
CGAACGTTTACTTCGAAAAG+TGG + chr7_2:84401281-84401300 Msa1084120:CDS 40.0%
TGTCTTTCTTGCTTGCTTCA+TGG + chr7_2:84401337-84401356 Msa1084120:intron 40.0%
GTTCCTTAACTAGTTGCAAG+AGG + chr7_2:84401411-84401430 Msa1084120:intron 40.0%
TCTCCTCTTGCAACTAGTTA+AGG - chr7_2:84401417-84401436 None:intergenic 40.0%
AATACCTTGAGGTTAGGTAG+TGG - chr7_2:84401456-84401475 None:intergenic 40.0%
ATGTCCGAAATCAAAGCTGA+GGG + chr7_2:84401901-84401920 Msa1084120:three_prime_UTR 40.0%
! ATCTCCCTCAGCTTTGATTT+CGG - chr7_2:84401908-84401927 None:intergenic 40.0%
TCACGCAGTTCAATTTGAAG+TGG - chr7_2:84401934-84401953 None:intergenic 40.0%
CACCATACAGGACACATAAT+TGG - chr7_2:84402007-84402026 None:intergenic 40.0%
CAAATTGTACCTCCAAGAGT+TGG + chr7_2:84402068-84402087 Msa1084120:three_prime_UTR 40.0%
AGTTGTCCATTTCCAACTCT+TGG - chr7_2:84402083-84402102 None:intergenic 40.0%
GGAAATGGACAACTACTGAA+AGG + chr7_2:84402089-84402108 Msa1084120:three_prime_UTR 40.0%
AGAGAAGAACCGGTCAATTT+CGG + chr7_2:84402182-84402201 Msa1084120:three_prime_UTR 40.0%
TTCAATTGGAGCAAGCATTC+GGG + chr7_2:84402230-84402249 Msa1084120:three_prime_UTR 40.0%
! AGGTAGAAGCGAACCTTTTA+AGG + chr7_2:84402254-84402273 Msa1084120:three_prime_UTR 40.0%
CGAACATTCAGGACCTTAAA+AGG - chr7_2:84402270-84402289 None:intergenic 40.0%
! TTTAAGGTCCTGAATGTTCG+AGG + chr7_2:84402270-84402289 Msa1084120:three_prime_UTR 40.0%
TGATAAAGTATGGCGTCTGA+AGG + chr7_2:84402429-84402448 Msa1084120:three_prime_UTR 40.0%
GATAAAGTATGGCGTCTGAA+GGG + chr7_2:84402430-84402449 Msa1084120:three_prime_UTR 40.0%
ATAAAGTATGGCGTCTGAAG+GGG + chr7_2:84402431-84402450 Msa1084120:three_prime_UTR 40.0%
GTAAAGATGGTACACTTCAC+AGG + chr7_2:84402458-84402477 Msa1084120:three_prime_UTR 40.0%
GTACAAACTGCACAATATGG+TGG + chr7_2:84403160-84403179 Msa1084120:three_prime_UTR 40.0%
TGGAAAGTCAAACTGTTGCA+GGG - chr7_2:84403197-84403216 None:intergenic 40.0%
TTGGAAAGTCAAACTGTTGC+AGG - chr7_2:84403198-84403217 None:intergenic 40.0%
CAGGCCTGAATGATAGAATT+AGG - chr7_2:84403377-84403396 None:intergenic 40.0%
!! ATTTCTGGTTGGCATGGAAA+TGG - chr7_2:84403430-84403449 None:intergenic 40.0%
! ATCTCACAAAGTGGACTTGT+AGG + chr7_2:84403466-84403485 Msa1084120:three_prime_UTR 40.0%
TGTGATCAAATGGCAGCATT+AGG + chr7_2:84403499-84403518 Msa1084120:three_prime_UTR 40.0%
ACCTCTTCTCCAGAAAAGTA+TGG - chr7_2:84403538-84403557 None:intergenic 40.0%
!! GTATCACTGGTTGGTCTATT+TGG + chr7_2:84403556-84403575 Msa1084120:three_prime_UTR 40.0%
TGCTCCGTCATCATAAACAA+AGG - chr7_2:84403614-84403633 None:intergenic 40.0%
!! TTTGTTTATGATGACGGAGC+AGG + chr7_2:84403613-84403632 Msa1084120:three_prime_UTR 40.0%
TGGATTGACCCTTAACGAGT+TGG + chr7_2:84401809-84401828 Msa1084120:CDS 45.0%
! TGTGTTGGCCAACTCGTTAA+GGG - chr7_2:84401820-84401839 None:intergenic 45.0%
! ATGTGTTGGCCAACTCGTTA+AGG - chr7_2:84401821-84401840 None:intergenic 45.0%
AGTTGGCCAACACATATGAG+AGG + chr7_2:84401826-84401845 Msa1084120:CDS 45.0%
GTCTCTCCTCTCATATGTGT+TGG - chr7_2:84401835-84401854 None:intergenic 45.0%
CATGTCCGAAATCAAAGCTG+AGG + chr7_2:84401900-84401919 Msa1084120:three_prime_UTR 45.0%
AAATCAGCAACGCGTAGTCA+TGG + chr7_2:84401959-84401978 Msa1084120:three_prime_UTR 45.0%
AAAAGAGGATTGGACAGCTG+AGG + chr7_2:84402034-84402053 Msa1084120:three_prime_UTR 45.0%
GTACCTCCAAGAGTTGGAAA+TGG + chr7_2:84402074-84402093 Msa1084120:three_prime_UTR 45.0%
TGTCCATTTCCAACTCTTGG+AGG - chr7_2:84402080-84402099 None:intergenic 45.0%
TAACCCTAGCCGAAATTGAC+CGG - chr7_2:84402194-84402213 None:intergenic 45.0%
GTCCTGAATGTTCGAGGAAA+GGG + chr7_2:84402276-84402295 Msa1084120:three_prime_UTR 45.0%
TACCCTTTCCTCGAACATTC+AGG - chr7_2:84402281-84402300 None:intergenic 45.0%
CTGAAGGGGATACGTAAAGA+TGG + chr7_2:84402445-84402464 Msa1084120:three_prime_UTR 45.0%
ACAAGACTGGAGGGAAGTTA+TGG + chr7_2:84402690-84402709 Msa1084120:three_prime_UTR 45.0%
GACTTTCCAATTGCTCAGCA+AGG + chr7_2:84403208-84403227 Msa1084120:three_prime_UTR 45.0%
! AGAGTACCTTGCTGAGCAAT+TGG - chr7_2:84403217-84403236 None:intergenic 45.0%
TAGGCGATGAGTCTTGAAGA+GGG + chr7_2:84403301-84403320 Msa1084120:intron 45.0%
TTCCATGCCAACCAGAAATG+TGG + chr7_2:84403431-84403450 Msa1084120:three_prime_UTR 45.0%
! ATGTCGTCGCCATACTTTTC+TGG + chr7_2:84403526-84403545 Msa1084120:three_prime_UTR 45.0%
!! GCCATACTTTTCTGGAGAAG+AGG + chr7_2:84403534-84403553 Msa1084120:three_prime_UTR 45.0%
!! TTCTGGAGAAGAGGTATCAC+TGG + chr7_2:84403543-84403562 Msa1084120:three_prime_UTR 45.0%
GGGGGAAGAATAATGATTGC+TGG - chr7_2:84403641-84403660 None:intergenic 45.0%
ATACCCAGTTAGCTAGCAGT+TGG + chr7_2:84404015-84404034 Msa1084120:three_prime_UTR 45.0%
CATCCAACTGCTAGCTAACT+GGG - chr7_2:84404021-84404040 None:intergenic 45.0%
!! ATCGAAGAGGGCATCTCATG+TGG + chr7_2:84400991-84401010 Msa1084120:CDS 50.0%
!! TCGAAGAGGGCATCTCATGT+GGG + chr7_2:84400992-84401011 Msa1084120:CDS 50.0%
! AGGTAGTGGTGGAGCTTATG+TGG - chr7_2:84401442-84401461 None:intergenic 50.0%
TCCACCACTACCTAACCTCA+AGG + chr7_2:84401449-84401468 Msa1084120:CDS 50.0%
! ACCTTGAGGTTAGGTAGTGG+TGG - chr7_2:84401453-84401472 None:intergenic 50.0%
CAACGCGTAGTCATGGAAGA+AGG + chr7_2:84401966-84401985 Msa1084120:three_prime_UTR 50.0%
AGAACCGGTCAATTTCGGCT+AGG + chr7_2:84402187-84402206 Msa1084120:three_prime_UTR 50.0%
GAACCGGTCAATTTCGGCTA+GGG + chr7_2:84402188-84402207 Msa1084120:three_prime_UTR 50.0%
ATTGGAGCAAGCATTCGGGA+AGG + chr7_2:84402234-84402253 Msa1084120:three_prime_UTR 50.0%
GGTCCTGAATGTTCGAGGAA+AGG + chr7_2:84402275-84402294 Msa1084120:three_prime_UTR 50.0%
CACAGGCAGTTGACATCAGA+TGG + chr7_2:84402475-84402494 Msa1084120:three_prime_UTR 50.0%
CTTCTGTCATTGGCAGTGCT+TGG + chr7_2:84403039-84403058 Msa1084120:intron 50.0%
CTAGGCGATGAGTCTTGAAG+AGG + chr7_2:84403300-84403319 Msa1084120:intron 50.0%
CTGGTTGGCATGGAAATGGT+AGG - chr7_2:84403426-84403445 None:intergenic 50.0%
!! TAGACGGCCACATTTCTGGT+TGG - chr7_2:84403441-84403460 None:intergenic 50.0%
GAGATAGACGGCCACATTTC+TGG - chr7_2:84403445-84403464 None:intergenic 50.0%
!! GGAGAAGAGGTATCACTGGT+TGG + chr7_2:84403547-84403566 Msa1084120:three_prime_UTR 50.0%
CCATCCAACTGCTAGCTAAC+TGG - chr7_2:84404022-84404041 None:intergenic 50.0%
CCAGTTAGCTAGCAGTTGGA+TGG + chr7_2:84404019-84404038 Msa1084120:three_prime_UTR 50.0%
CTGTCATTGGCAGTGCTTGG+TGG + chr7_2:84403042-84403061 Msa1084120:intron 55.0%
!! GGCCACATTTCTGGTTGGCA+TGG - chr7_2:84403436-84403455 None:intergenic 55.0%
TAGCTAGCAGTTGGATGGCC+TGG + chr7_2:84404024-84404043 Msa1084120:three_prime_UTR 55.0%
AGCTAGCAGTTGGATGGCCT+GGG + chr7_2:84404025-84404044 Msa1084120:three_prime_UTR 55.0%
GCAAATGACAAAGCAGCCCC+AGG - chr7_2:84404045-84404064 None:intergenic 55.0%
GCTAGCAGTTGGATGGCCTG+GGG + chr7_2:84404026-84404045 Msa1084120:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 84400863 84404135 84400863 ID=Msa1084120;Name=Msa1084120
chr7_2 mRNA 84400863 84404135 84400863 ID=Msa1084120-mRNA-1;Parent=Msa1084120;Name=Msa1084120-mRNA-1;_AED=0.28;_eAED=0.28;_QI=123|1|1|1|0.85|0.87|8|1712|105
chr7_2 exon 84400863 84401125 84400863 ID=Msa1084120-mRNA-1:exon:22521;Parent=Msa1084120-mRNA-1
chr7_2 exon 84401242 84401302 84401242 ID=Msa1084120-mRNA-1:exon:22522;Parent=Msa1084120-mRNA-1
chr7_2 exon 84401424 84401470 84401424 ID=Msa1084120-mRNA-1:exon:22523;Parent=Msa1084120-mRNA-1
chr7_2 exon 84401806 84402297 84401806 ID=Msa1084120-mRNA-1:exon:22524;Parent=Msa1084120-mRNA-1
chr7_2 exon 84402398 84402558 84402398 ID=Msa1084120-mRNA-1:exon:22525;Parent=Msa1084120-mRNA-1
chr7_2 exon 84402683 84402892 84402683 ID=Msa1084120-mRNA-1:exon:22526;Parent=Msa1084120-mRNA-1
chr7_2 exon 84403055 84403229 84403055 ID=Msa1084120-mRNA-1:exon:22527;Parent=Msa1084120-mRNA-1
chr7_2 exon 84403392 84404135 84403392 ID=Msa1084120-mRNA-1:exon:22528;Parent=Msa1084120-mRNA-1
chr7_2 five_prime_UTR 84400863 84400985 84400863 ID=Msa1084120-mRNA-1:five_prime_utr;Parent=Msa1084120-mRNA-1
chr7_2 CDS 84400986 84401125 84400986 ID=Msa1084120-mRNA-1:cds;Parent=Msa1084120-mRNA-1
chr7_2 CDS 84401242 84401302 84401242 ID=Msa1084120-mRNA-1:cds;Parent=Msa1084120-mRNA-1
chr7_2 CDS 84401424 84401470 84401424 ID=Msa1084120-mRNA-1:cds;Parent=Msa1084120-mRNA-1
chr7_2 CDS 84401806 84401875 84401806 ID=Msa1084120-mRNA-1:cds;Parent=Msa1084120-mRNA-1
chr7_2 three_prime_UTR 84401876 84402297 84401876 ID=Msa1084120-mRNA-1:three_prime_utr;Parent=Msa1084120-mRNA-1
chr7_2 three_prime_UTR 84402398 84402558 84402398 ID=Msa1084120-mRNA-1:three_prime_utr;Parent=Msa1084120-mRNA-1
chr7_2 three_prime_UTR 84402683 84402892 84402683 ID=Msa1084120-mRNA-1:three_prime_utr;Parent=Msa1084120-mRNA-1
chr7_2 three_prime_UTR 84403055 84403229 84403055 ID=Msa1084120-mRNA-1:three_prime_utr;Parent=Msa1084120-mRNA-1
chr7_2 three_prime_UTR 84403392 84404135 84403392 ID=Msa1084120-mRNA-1:three_prime_utr;Parent=Msa1084120-mRNA-1
Gene Sequence

>Msa1084120

ATGGCATCGAAGAGGGCATCTCATGTGGGTAGTTCTAGTAGTGATGACAAGGATCTTCATGTTCCTTCTACTAGTACTAAGAGAATAAAACATGGGAATCCAGAAGAACAACAAAAAGAGATCCAACACCTTTTATTTCCCACTAATAGCGCCGCCAGTAACAATCTTGCGTTTTGCTTCGAACGTTTACTTCGAAAAGTGTTGCAAGAGGAGATTCCACATAAGCTCCACCACTACCTAACCTCAAGCTGTGGATTGACCCTTAACGAGTTGGCCAACACATATGAGAGGAGAGACGTTTGCAATTGTGTTTCATGA
Protein sequence

>Msa1084120

MASKRASHVGSSSSDDKDLHVPSTSTKRIKHGNPEEQQKEIQHLLFPTNSAASNNLAFCFERLLRKVLQEEIPHKLHHYLTSSCGLTLNELANTYERRDVCNCVS*