AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1181840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 48 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCCGAAAATAAGTGTTAT+TGG 0.158167 8_1:+16469965 Msa1181840:CDS
TATAAGGTTCTTGCTGCTTT+TGG 0.206713 8_1:+16471049 Msa1181840:three_prime_UTR
CATTGGCTACCTATACTTTC+TGG 0.232813 8_1:-16471415 None:intergenic
TGCATGGGCCAAGGACAAAA+TGG 0.256606 8_1:-16470017 None:intergenic
ACCTCTTGCACTTTCATTCC+AGG 0.276500 8_1:-16471325 None:intergenic
TTAGGCTTTGTTTGGATTGA+TGG 0.303770 8_1:+16471523 Msa1181840:three_prime_UTR
AAATTCGACTTATTTATATA+AGG 0.331569 8_1:+16471033 Msa1181840:three_prime_UTR
GATTCTCTTCTTGCTGCACC+TGG 0.403105 8_1:+16471307 Msa1181840:three_prime_UTR
AGCTGCTGGTGGTGGTGAGT+GGG 0.408495 8_1:+16469911 Msa1181840:CDS
CAGCTCGGGTATCTGTCATT+TGG 0.429102 8_1:-16471466 None:intergenic
ATGATTGTTGTTGCAGCTGC+TGG 0.435795 8_1:+16469897 Msa1181840:CDS
GGTATATATATGTAGGGGTG+TGG 0.443517 8_1:+16469935 Msa1181840:intron
TTAATCATGATGAGTCTTTG+AGG 0.448488 8_1:+16471496 Msa1181840:three_prime_UTR
ATTCAGTCTGATCATTCTCC+TGG 0.449671 8_1:+16470439 Msa1181840:three_prime_UTR
GTCTGTCATTTGGACATCTC+CGG 0.462051 8_1:-16471350 None:intergenic
CAGCTGCTGGTGGTGGTGAG+TGG 0.464829 8_1:+16469910 Msa1181840:CDS
ATGAAAGTGCAAGAGGTTGC+CGG 0.475013 8_1:+16471331 Msa1181840:three_prime_UTR
GTATATATATGTAGGGGTGT+GGG 0.484437 8_1:+16469936 Msa1181840:intron
GTTGTTGCAGCTGCTGGTGG+TGG 0.494223 8_1:+16469903 Msa1181840:CDS
GGCCAAGGACAAAATGGGTA+TGG 0.509784 8_1:-16470011 None:intergenic
ATGATTAAGCATCATCAGCT+CGG 0.512053 8_1:-16471481 None:intergenic
GCATGGGCCAAGGACAAAAT+GGG 0.518955 8_1:-16470016 None:intergenic
TGCTGGTGGTGGTGAGTGGG+TGG 0.521183 8_1:+16469914 Msa1181840:CDS
TGTCGGTGGAGATCCGCCTT+CGG 0.527261 8_1:+16470126 Msa1181840:CDS
TGATTAAGCATCATCAGCTC+GGG 0.537911 8_1:-16471480 None:intergenic
GTTAATGTGCAAGAGGTTGT+CGG 0.545673 8_1:+16471437 Msa1181840:three_prime_UTR
CAGACGGAGTAGATGCGGTA+TGG 0.550164 8_1:-16470105 None:intergenic
TAAACACTATCACTACTGCA+TGG 0.551096 8_1:-16470033 None:intergenic
AGGCCGCGACCAGAAAGTAT+AGG 0.551913 8_1:+16471406 Msa1181840:three_prime_UTR
AACCTCTTGCACATTAACAT+TGG 0.559132 8_1:-16471432 None:intergenic
AAACACTATCACTACTGCAT+GGG 0.560477 8_1:-16470032 None:intergenic
CTACCTATACTTTCTGGTCG+CGG 0.569121 8_1:-16471409 None:intergenic
ATTGATGGAATATGATGTAA+TGG 0.571482 8_1:+16471538 Msa1181840:three_prime_UTR
TATCACTACTGCATGGGCCA+AGG 0.585064 8_1:-16470026 None:intergenic
GGCGGATCTCCACCGACAGA+CGG 0.593030 8_1:-16470121 None:intergenic
AACTTACATAAAGCCGAAGG+CGG 0.606746 8_1:-16470139 None:intergenic
ATTGTTGTTGCAGCTGCTGG+TGG 0.607347 8_1:+16469900 Msa1181840:CDS
AGTGATAGTGTTTAGCGTTG+CGG 0.608609 8_1:+16470042 Msa1181840:CDS
AGCCAATGTTAATGTGCAAG+AGG 0.609445 8_1:+16471430 Msa1181840:three_prime_UTR
GCATCTACTCCGTCTGTCGG+TGG 0.633547 8_1:+16470112 Msa1181840:CDS
GTTCTCGATGAAGCTGAACA+AGG 0.634951 8_1:+16471088 Msa1181840:three_prime_UTR
TGGAGTTTAACTTACCGACA+TGG 0.637122 8_1:-16470289 None:intergenic
ACCGCATCTACTCCGTCTGT+CGG 0.655275 8_1:+16470109 Msa1181840:CDS
ACCGACAGACGGAGTAGATG+CGG 0.659716 8_1:-16470110 None:intergenic
ACCTGGAATGAAAGTGCAAG+AGG 0.674183 8_1:+16471324 Msa1181840:three_prime_UTR
GAGAACTTACATAAAGCCGA+AGG 0.693598 8_1:-16470142 None:intergenic
AGGGTGACACTATCATGTGT+AGG 0.706105 8_1:-16471701 None:intergenic
TTATAAGGAAGACTCCATGT+CGG 0.734707 8_1:+16470275 Msa1181840:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAATTATTGTCTTAAA+TGG + chr8_1:16471006-16471025 Msa1181840:intron 10.0%
!! AAAATAATTATTGTCTTAAA+TGG + chr8_1:16471006-16471025 Msa1181840:intron 10.0%
!! TTTAATATGAGTTAAGTTAA+TGG - chr8_1:16470708-16470727 None:intergenic 15.0%
!!! AAAATAAATACATTCACTTT+TGG - chr8_1:16470740-16470759 None:intergenic 15.0%
!! AAATTCGACTTATTTATATA+AGG + chr8_1:16471033-16471052 Msa1181840:three_prime_UTR 15.0%
!! TTTAATATGAGTTAAGTTAA+TGG - chr8_1:16470708-16470727 None:intergenic 15.0%
!!! AAAATAAATACATTCACTTT+TGG - chr8_1:16470740-16470759 None:intergenic 15.0%
!! AAATTCGACTTATTTATATA+AGG + chr8_1:16471033-16471052 Msa1181840:three_prime_UTR 15.0%
!! TTAACTCATATTAAAACGAA+GGG + chr8_1:16470713-16470732 Msa1181840:intron 20.0%
!! TAATTATAATCTCTGAATAG+CGG + chr8_1:16470762-16470781 Msa1181840:intron 20.0%
!!! ACTGATTAGGTTTTTTATTT+AGG + chr8_1:16470927-16470946 Msa1181840:intron 20.0%
!!! ACAATAATTATTTTCTCACA+CGG - chr8_1:16471000-16471019 None:intergenic 20.0%
!! AATCATCATAAAGTGAAAAA+AGG - chr8_1:16471577-16471596 None:intergenic 20.0%
!!! TTTCTACATGTGTTTTTAAA+AGG + chr8_1:16471667-16471686 Msa1181840:three_prime_UTR 20.0%
!!! TTCTACATGTGTTTTTAAAA+GGG + chr8_1:16471668-16471687 Msa1181840:three_prime_UTR 20.0%
!! TTAACTCATATTAAAACGAA+GGG + chr8_1:16470713-16470732 Msa1181840:intron 20.0%
!! TAATTATAATCTCTGAATAG+CGG + chr8_1:16470762-16470781 Msa1181840:intron 20.0%
!!! ACTGATTAGGTTTTTTATTT+AGG + chr8_1:16470927-16470946 Msa1181840:intron 20.0%
!!! ACAATAATTATTTTCTCACA+CGG - chr8_1:16471000-16471019 None:intergenic 20.0%
!! AATCATCATAAAGTGAAAAA+AGG - chr8_1:16471577-16471596 None:intergenic 20.0%
!!! TTTCTACATGTGTTTTTAAA+AGG + chr8_1:16471667-16471686 Msa1181840:three_prime_UTR 20.0%
!!! TTCTACATGTGTTTTTAAAA+GGG + chr8_1:16471668-16471687 Msa1181840:three_prime_UTR 20.0%
! TTTCCGAAAATAAGTGTTAT+TGG + chr8_1:16469965-16469984 Msa1181840:CDS 25.0%
!!! TAGCCAATAACACTTATTTT+CGG - chr8_1:16469971-16469990 None:intergenic 25.0%
!! GTGATGAGTTTTTATTGTTA+GGG - chr8_1:16470071-16470090 None:intergenic 25.0%
!! TGTGATGAGTTTTTATTGTT+AGG - chr8_1:16470072-16470091 None:intergenic 25.0%
! TGAAAGATAAGAGATTGTTA+AGG - chr8_1:16470219-16470238 None:intergenic 25.0%
!!! TCCTTTTCAATTTCAAGTTT+AGG + chr8_1:16470518-16470537 Msa1181840:intron 25.0%
!!! CCTTTTCAATTTCAAGTTTA+GGG + chr8_1:16470519-16470538 Msa1181840:intron 25.0%
!! ATTAAGCTTTTTATCCAATC+AGG + chr8_1:16470631-16470650 Msa1181840:intron 25.0%
!! TTAAGCTTTTTATCCAATCA+GGG + chr8_1:16470632-16470651 Msa1181840:intron 25.0%
! AATATGAGTTAAGTTAATGG+AGG - chr8_1:16470705-16470724 None:intergenic 25.0%
! CTTAACTCATATTAAAACGA+AGG + chr8_1:16470712-16470731 Msa1181840:intron 25.0%
! AATTATAATCTCTGAATAGC+GGG + chr8_1:16470763-16470782 Msa1181840:intron 25.0%
! ATCATTTGCAAAACAAAGAA+AGG - chr8_1:16470888-16470907 None:intergenic 25.0%
!! CATTTTTCATACAACTGATT+AGG + chr8_1:16470914-16470933 Msa1181840:intron 25.0%
! TTTCTAACATTATGATTGAG+AGG + chr8_1:16470966-16470985 Msa1181840:intron 25.0%
! TAATTATTGTCTTAAATGGC+AGG + chr8_1:16471010-16471029 Msa1181840:intron 25.0%
!!! TTTTGGAATTAATGTGGTAA+AGG + chr8_1:16471066-16471085 Msa1181840:three_prime_UTR 25.0%
!! ACAATCTTTTACCAAGAAAA+AGG + chr8_1:16471386-16471405 Msa1181840:three_prime_UTR 25.0%
! ATTGATGGAATATGATGTAA+TGG + chr8_1:16471538-16471557 Msa1181840:three_prime_UTR 25.0%
! TTTCCGAAAATAAGTGTTAT+TGG + chr8_1:16469965-16469984 Msa1181840:CDS 25.0%
!!! TAGCCAATAACACTTATTTT+CGG - chr8_1:16469971-16469990 None:intergenic 25.0%
!! GTGATGAGTTTTTATTGTTA+GGG - chr8_1:16470071-16470090 None:intergenic 25.0%
!! TGTGATGAGTTTTTATTGTT+AGG - chr8_1:16470072-16470091 None:intergenic 25.0%
! TGAAAGATAAGAGATTGTTA+AGG - chr8_1:16470219-16470238 None:intergenic 25.0%
!!! TCCTTTTCAATTTCAAGTTT+AGG + chr8_1:16470518-16470537 Msa1181840:intron 25.0%
!!! CCTTTTCAATTTCAAGTTTA+GGG + chr8_1:16470519-16470538 Msa1181840:intron 25.0%
!! ATTAAGCTTTTTATCCAATC+AGG + chr8_1:16470631-16470650 Msa1181840:intron 25.0%
!! TTAAGCTTTTTATCCAATCA+GGG + chr8_1:16470632-16470651 Msa1181840:intron 25.0%
! AATATGAGTTAAGTTAATGG+AGG - chr8_1:16470705-16470724 None:intergenic 25.0%
! CTTAACTCATATTAAAACGA+AGG + chr8_1:16470712-16470731 Msa1181840:intron 25.0%
! AATTATAATCTCTGAATAGC+GGG + chr8_1:16470763-16470782 Msa1181840:intron 25.0%
! ATCATTTGCAAAACAAAGAA+AGG - chr8_1:16470888-16470907 None:intergenic 25.0%
!! CATTTTTCATACAACTGATT+AGG + chr8_1:16470914-16470933 Msa1181840:intron 25.0%
! TTTCTAACATTATGATTGAG+AGG + chr8_1:16470966-16470985 Msa1181840:intron 25.0%
! TAATTATTGTCTTAAATGGC+AGG + chr8_1:16471010-16471029 Msa1181840:intron 25.0%
!!! TTTTGGAATTAATGTGGTAA+AGG + chr8_1:16471066-16471085 Msa1181840:three_prime_UTR 25.0%
!! ACAATCTTTTACCAAGAAAA+AGG + chr8_1:16471386-16471405 Msa1181840:three_prime_UTR 25.0%
! ATTGATGGAATATGATGTAA+TGG + chr8_1:16471538-16471557 Msa1181840:three_prime_UTR 25.0%
! CTTTTCGTGTGTAGATTATA+AGG + chr8_1:16470260-16470279 Msa1181840:CDS 30.0%
ACTCAATTCAAACAACAACA+TGG - chr8_1:16470312-16470331 None:intergenic 30.0%
AAGAAATTCTGAATCAAACC+AGG - chr8_1:16470460-16470479 None:intergenic 30.0%
CCCTAAACTTGAAATTGAAA+AGG - chr8_1:16470522-16470541 None:intergenic 30.0%
AAATAAAGACTAACCCTGAT+TGG - chr8_1:16470648-16470667 None:intergenic 30.0%
CTAAAAACTTGTCTGTCATT+TGG - chr8_1:16471363-16471382 None:intergenic 30.0%
TTAATCATGATGAGTCTTTG+AGG + chr8_1:16471496-16471515 Msa1181840:three_prime_UTR 30.0%
! CTTTTCGTGTGTAGATTATA+AGG + chr8_1:16470260-16470279 Msa1181840:CDS 30.0%
ACTCAATTCAAACAACAACA+TGG - chr8_1:16470312-16470331 None:intergenic 30.0%
AAGAAATTCTGAATCAAACC+AGG - chr8_1:16470460-16470479 None:intergenic 30.0%
CCCTAAACTTGAAATTGAAA+AGG - chr8_1:16470522-16470541 None:intergenic 30.0%
AAATAAAGACTAACCCTGAT+TGG - chr8_1:16470648-16470667 None:intergenic 30.0%
CTAAAAACTTGTCTGTCATT+TGG - chr8_1:16471363-16471382 None:intergenic 30.0%
TTAATCATGATGAGTCTTTG+AGG + chr8_1:16471496-16471515 Msa1181840:three_prime_UTR 30.0%
AGTGGGTGGTATATATATGT+AGG + chr8_1:16469928-16469947 Msa1181840:intron 35.0%
GTGGGTGGTATATATATGTA+GGG + chr8_1:16469929-16469948 Msa1181840:intron 35.0%
TGGGTGGTATATATATGTAG+GGG + chr8_1:16469930-16469949 Msa1181840:intron 35.0%
! GTATATATATGTAGGGGTGT+GGG + chr8_1:16469936-16469955 Msa1181840:intron 35.0%
AAACACTATCACTACTGCAT+GGG - chr8_1:16470035-16470054 None:intergenic 35.0%
TAAACACTATCACTACTGCA+TGG - chr8_1:16470036-16470055 None:intergenic 35.0%
TTATAAGGAAGACTCCATGT+CGG + chr8_1:16470275-16470294 Msa1181840:exon 35.0%
! TTTTCAGTCTTGGATGATCA+GGG + chr8_1:16470403-16470422 Msa1181840:three_prime_UTR 35.0%
!! TTTCAAGTTTAGGGTCTGTT+TGG + chr8_1:16470528-16470547 Msa1181840:intron 35.0%
!!! TATAAGGTTCTTGCTGCTTT+TGG + chr8_1:16471049-16471068 Msa1181840:three_prime_UTR 35.0%
!!! TGCTGCTTTTGGAATTAATG+TGG + chr8_1:16471060-16471079 Msa1181840:three_prime_UTR 35.0%
TTGTTGTGTCTGAACTTTGT+AGG + chr8_1:16471268-16471287 Msa1181840:intron 35.0%
AACCTCTTGCACATTAACAT+TGG - chr8_1:16471435-16471454 None:intergenic 35.0%
ATGATTAAGCATCATCAGCT+CGG - chr8_1:16471484-16471503 None:intergenic 35.0%
!! ATGAGTCTTTGAGGAGTTTT+AGG + chr8_1:16471505-16471524 Msa1181840:three_prime_UTR 35.0%
!! TTAGGCTTTGTTTGGATTGA+TGG + chr8_1:16471523-16471542 Msa1181840:three_prime_UTR 35.0%
AGTGGGTGGTATATATATGT+AGG + chr8_1:16469928-16469947 Msa1181840:intron 35.0%
GTGGGTGGTATATATATGTA+GGG + chr8_1:16469929-16469948 Msa1181840:intron 35.0%
TGGGTGGTATATATATGTAG+GGG + chr8_1:16469930-16469949 Msa1181840:intron 35.0%
! GTATATATATGTAGGGGTGT+GGG + chr8_1:16469936-16469955 Msa1181840:intron 35.0%
AAACACTATCACTACTGCAT+GGG - chr8_1:16470035-16470054 None:intergenic 35.0%
TAAACACTATCACTACTGCA+TGG - chr8_1:16470036-16470055 None:intergenic 35.0%
TTATAAGGAAGACTCCATGT+CGG + chr8_1:16470275-16470294 Msa1181840:exon 35.0%
! TTTTCAGTCTTGGATGATCA+GGG + chr8_1:16470403-16470422 Msa1181840:three_prime_UTR 35.0%
!! TTTCAAGTTTAGGGTCTGTT+TGG + chr8_1:16470528-16470547 Msa1181840:intron 35.0%
!!! TATAAGGTTCTTGCTGCTTT+TGG + chr8_1:16471049-16471068 Msa1181840:three_prime_UTR 35.0%
!!! TGCTGCTTTTGGAATTAATG+TGG + chr8_1:16471060-16471079 Msa1181840:three_prime_UTR 35.0%
TTGTTGTGTCTGAACTTTGT+AGG + chr8_1:16471268-16471287 Msa1181840:intron 35.0%
AACCTCTTGCACATTAACAT+TGG - chr8_1:16471435-16471454 None:intergenic 35.0%
ATGATTAAGCATCATCAGCT+CGG - chr8_1:16471484-16471503 None:intergenic 35.0%
!! ATGAGTCTTTGAGGAGTTTT+AGG + chr8_1:16471505-16471524 Msa1181840:three_prime_UTR 35.0%
!! TTAGGCTTTGTTTGGATTGA+TGG + chr8_1:16471523-16471542 Msa1181840:three_prime_UTR 35.0%
GGTATATATATGTAGGGGTG+TGG + chr8_1:16469935-16469954 Msa1181840:intron 40.0%
! ATCCATACCCATTTTGTCCT+TGG + chr8_1:16470009-16470028 Msa1181840:CDS 40.0%
! AGTGATAGTGTTTAGCGTTG+CGG + chr8_1:16470042-16470061 Msa1181840:CDS 40.0%
AACTTACATAAAGCCGAAGG+CGG - chr8_1:16470142-16470161 None:intergenic 40.0%
GAGAACTTACATAAAGCCGA+AGG - chr8_1:16470145-16470164 None:intergenic 40.0%
! TGGAGTTTAACTTACCGACA+TGG - chr8_1:16470292-16470311 None:intergenic 40.0%
! GCAGTTTACGTTTTCAGTCT+TGG + chr8_1:16470393-16470412 Msa1181840:intron 40.0%
! GTTTTCAGTCTTGGATGATC+AGG + chr8_1:16470402-16470421 Msa1181840:three_prime_UTR 40.0%
ATTCAGTCTGATCATTCTCC+TGG + chr8_1:16470439-16470458 Msa1181840:three_prime_UTR 40.0%
! GTTTAGGGTCTGTTTGGATT+AGG + chr8_1:16470534-16470553 Msa1181840:intron 40.0%
ATCACTATTAAGTCCACCAG+AGG + chr8_1:16471112-16471131 Msa1181840:intron 40.0%
CATTTGAAAAAGAGAGCCTC+TGG - chr8_1:16471131-16471150 None:intergenic 40.0%
! CATTGGCTACCTATACTTTC+TGG - chr8_1:16471418-16471437 None:intergenic 40.0%
AGCCAATGTTAATGTGCAAG+AGG + chr8_1:16471430-16471449 Msa1181840:three_prime_UTR 40.0%
GTTAATGTGCAAGAGGTTGT+CGG + chr8_1:16471437-16471456 Msa1181840:three_prime_UTR 40.0%
TGATTAAGCATCATCAGCTC+GGG - chr8_1:16471483-16471502 None:intergenic 40.0%
!!! GAGGAGTTTTAGGCTTTGTT+TGG + chr8_1:16471515-16471534 Msa1181840:three_prime_UTR 40.0%
GGTATATATATGTAGGGGTG+TGG + chr8_1:16469935-16469954 Msa1181840:intron 40.0%
! ATCCATACCCATTTTGTCCT+TGG + chr8_1:16470009-16470028 Msa1181840:CDS 40.0%
! AGTGATAGTGTTTAGCGTTG+CGG + chr8_1:16470042-16470061 Msa1181840:CDS 40.0%
AACTTACATAAAGCCGAAGG+CGG - chr8_1:16470142-16470161 None:intergenic 40.0%
GAGAACTTACATAAAGCCGA+AGG - chr8_1:16470145-16470164 None:intergenic 40.0%
! TGGAGTTTAACTTACCGACA+TGG - chr8_1:16470292-16470311 None:intergenic 40.0%
! GCAGTTTACGTTTTCAGTCT+TGG + chr8_1:16470393-16470412 Msa1181840:intron 40.0%
! GTTTTCAGTCTTGGATGATC+AGG + chr8_1:16470402-16470421 Msa1181840:three_prime_UTR 40.0%
ATTCAGTCTGATCATTCTCC+TGG + chr8_1:16470439-16470458 Msa1181840:three_prime_UTR 40.0%
! GTTTAGGGTCTGTTTGGATT+AGG + chr8_1:16470534-16470553 Msa1181840:intron 40.0%
ATCACTATTAAGTCCACCAG+AGG + chr8_1:16471112-16471131 Msa1181840:intron 40.0%
CATTTGAAAAAGAGAGCCTC+TGG - chr8_1:16471131-16471150 None:intergenic 40.0%
! CATTGGCTACCTATACTTTC+TGG - chr8_1:16471418-16471437 None:intergenic 40.0%
AGCCAATGTTAATGTGCAAG+AGG + chr8_1:16471430-16471449 Msa1181840:three_prime_UTR 40.0%
GTTAATGTGCAAGAGGTTGT+CGG + chr8_1:16471437-16471456 Msa1181840:three_prime_UTR 40.0%
TGATTAAGCATCATCAGCTC+GGG - chr8_1:16471483-16471502 None:intergenic 40.0%
!!! GAGGAGTTTTAGGCTTTGTT+TGG + chr8_1:16471515-16471534 Msa1181840:three_prime_UTR 40.0%
! ATGATTGTTGTTGCAGCTGC+TGG + chr8_1:16469897-16469916 Msa1181840:CDS 45.0%
ATGGTAAAGCGGGTCAATAG+CGG + chr8_1:16470811-16470830 Msa1181840:intron 45.0%
GTTCTCGATGAAGCTGAACA+AGG + chr8_1:16471088-16471107 Msa1181840:three_prime_UTR 45.0%
TTGAAAAAGAGAGCCTCTGG+TGG - chr8_1:16471128-16471147 None:intergenic 45.0%
! ACCTGGAATGAAAGTGCAAG+AGG + chr8_1:16471324-16471343 Msa1181840:three_prime_UTR 45.0%
ACCTCTTGCACTTTCATTCC+AGG - chr8_1:16471328-16471347 None:intergenic 45.0%
! ATGAAAGTGCAAGAGGTTGC+CGG + chr8_1:16471331-16471350 Msa1181840:three_prime_UTR 45.0%
! GTCTGTCATTTGGACATCTC+CGG - chr8_1:16471353-16471372 None:intergenic 45.0%
! CTACCTATACTTTCTGGTCG+CGG - chr8_1:16471412-16471431 None:intergenic 45.0%
! ATGATTGTTGTTGCAGCTGC+TGG + chr8_1:16469897-16469916 Msa1181840:CDS 45.0%
ATGGTAAAGCGGGTCAATAG+CGG + chr8_1:16470811-16470830 Msa1181840:intron 45.0%
GTTCTCGATGAAGCTGAACA+AGG + chr8_1:16471088-16471107 Msa1181840:three_prime_UTR 45.0%
TTGAAAAAGAGAGCCTCTGG+TGG - chr8_1:16471128-16471147 None:intergenic 45.0%
! ACCTGGAATGAAAGTGCAAG+AGG + chr8_1:16471324-16471343 Msa1181840:three_prime_UTR 45.0%
ACCTCTTGCACTTTCATTCC+AGG - chr8_1:16471328-16471347 None:intergenic 45.0%
! ATGAAAGTGCAAGAGGTTGC+CGG + chr8_1:16471331-16471350 Msa1181840:three_prime_UTR 45.0%
! GTCTGTCATTTGGACATCTC+CGG - chr8_1:16471353-16471372 None:intergenic 45.0%
! CTACCTATACTTTCTGGTCG+CGG - chr8_1:16471412-16471431 None:intergenic 45.0%
! ATTGTTGTTGCAGCTGCTGG+TGG + chr8_1:16469900-16469919 Msa1181840:CDS 50.0%
GGCCAAGGACAAAATGGGTA+TGG - chr8_1:16470014-16470033 None:intergenic 50.0%
GCATGGGCCAAGGACAAAAT+GGG - chr8_1:16470019-16470038 None:intergenic 50.0%
TGCATGGGCCAAGGACAAAA+TGG - chr8_1:16470020-16470039 None:intergenic 50.0%
TATCACTACTGCATGGGCCA+AGG - chr8_1:16470029-16470048 None:intergenic 50.0%
CTTTACCATAACGCGCGACT+CGG - chr8_1:16470800-16470819 None:intergenic 50.0%
AGTCGCGCGTTATGGTAAAG+CGG + chr8_1:16470800-16470819 Msa1181840:intron 50.0%
TGGTAAAGCGGGTCAATAGC+GGG + chr8_1:16470812-16470831 Msa1181840:intron 50.0%
GATTCTCTTCTTGCTGCACC+TGG + chr8_1:16471307-16471326 Msa1181840:three_prime_UTR 50.0%
CAGCTCGGGTATCTGTCATT+TGG - chr8_1:16471469-16471488 None:intergenic 50.0%
! ATTGTTGTTGCAGCTGCTGG+TGG + chr8_1:16469900-16469919 Msa1181840:CDS 50.0%
GGCCAAGGACAAAATGGGTA+TGG - chr8_1:16470014-16470033 None:intergenic 50.0%
GCATGGGCCAAGGACAAAAT+GGG - chr8_1:16470019-16470038 None:intergenic 50.0%
TGCATGGGCCAAGGACAAAA+TGG - chr8_1:16470020-16470039 None:intergenic 50.0%
TATCACTACTGCATGGGCCA+AGG - chr8_1:16470029-16470048 None:intergenic 50.0%
CTTTACCATAACGCGCGACT+CGG - chr8_1:16470800-16470819 None:intergenic 50.0%
AGTCGCGCGTTATGGTAAAG+CGG + chr8_1:16470800-16470819 Msa1181840:intron 50.0%
TGGTAAAGCGGGTCAATAGC+GGG + chr8_1:16470812-16470831 Msa1181840:intron 50.0%
GATTCTCTTCTTGCTGCACC+TGG + chr8_1:16471307-16471326 Msa1181840:three_prime_UTR 50.0%
CAGCTCGGGTATCTGTCATT+TGG - chr8_1:16471469-16471488 None:intergenic 50.0%
CAGACGGAGTAGATGCGGTA+TGG - chr8_1:16470108-16470127 None:intergenic 55.0%
ACCGCATCTACTCCGTCTGT+CGG + chr8_1:16470109-16470128 Msa1181840:CDS 55.0%
ACCGACAGACGGAGTAGATG+CGG - chr8_1:16470113-16470132 None:intergenic 55.0%
GTCGCGCGTTATGGTAAAGC+GGG + chr8_1:16470801-16470820 Msa1181840:intron 55.0%
! TCTGGTCGCGGCCTTTTTCT+TGG - chr8_1:16471400-16471419 None:intergenic 55.0%
! AGGCCGCGACCAGAAAGTAT+AGG + chr8_1:16471406-16471425 Msa1181840:three_prime_UTR 55.0%
CAGACGGAGTAGATGCGGTA+TGG - chr8_1:16470108-16470127 None:intergenic 55.0%
ACCGCATCTACTCCGTCTGT+CGG + chr8_1:16470109-16470128 Msa1181840:CDS 55.0%
ACCGACAGACGGAGTAGATG+CGG - chr8_1:16470113-16470132 None:intergenic 55.0%
GTCGCGCGTTATGGTAAAGC+GGG + chr8_1:16470801-16470820 Msa1181840:intron 55.0%
! TCTGGTCGCGGCCTTTTTCT+TGG - chr8_1:16471400-16471419 None:intergenic 55.0%
! AGGCCGCGACCAGAAAGTAT+AGG + chr8_1:16471406-16471425 Msa1181840:three_prime_UTR 55.0%
GTTGTTGCAGCTGCTGGTGG+TGG + chr8_1:16469903-16469922 Msa1181840:CDS 60.0%
!! AGCTGCTGGTGGTGGTGAGT+GGG + chr8_1:16469911-16469930 Msa1181840:CDS 60.0%
GCATCTACTCCGTCTGTCGG+TGG + chr8_1:16470112-16470131 Msa1181840:CDS 60.0%
TGTCGGTGGAGATCCGCCTT+CGG + chr8_1:16470126-16470145 Msa1181840:CDS 60.0%
GGAGTGGGGCGGATCAATAG+CGG + chr8_1:16470853-16470872 Msa1181840:intron 60.0%
GAGTGGGGCGGATCAATAGC+GGG + chr8_1:16470854-16470873 Msa1181840:intron 60.0%
GTTGTTGCAGCTGCTGGTGG+TGG + chr8_1:16469903-16469922 Msa1181840:CDS 60.0%
!! AGCTGCTGGTGGTGGTGAGT+GGG + chr8_1:16469911-16469930 Msa1181840:CDS 60.0%
GCATCTACTCCGTCTGTCGG+TGG + chr8_1:16470112-16470131 Msa1181840:CDS 60.0%
TGTCGGTGGAGATCCGCCTT+CGG + chr8_1:16470126-16470145 Msa1181840:CDS 60.0%
GGAGTGGGGCGGATCAATAG+CGG + chr8_1:16470853-16470872 Msa1181840:intron 60.0%
GAGTGGGGCGGATCAATAGC+GGG + chr8_1:16470854-16470873 Msa1181840:intron 60.0%
!! CAGCTGCTGGTGGTGGTGAG+TGG + chr8_1:16469910-16469929 Msa1181840:CDS 65.0%
!! TGCTGGTGGTGGTGAGTGGG+TGG + chr8_1:16469914-16469933 Msa1181840:CDS 65.0%
GGCGGATCTCCACCGACAGA+CGG - chr8_1:16470124-16470143 None:intergenic 65.0%
CGCTACCGAGTCGCGCGTTA+TGG + chr8_1:16470792-16470811 Msa1181840:intron 65.0%
!! AGCGGGTCAATAGCGGGTCG+AGG + chr8_1:16470818-16470837 Msa1181840:intron 65.0%
ATTGATCCGCCCCACTCCGC+CGG - chr8_1:16470851-16470870 None:intergenic 65.0%
!! CAGCTGCTGGTGGTGGTGAG+TGG + chr8_1:16469910-16469929 Msa1181840:CDS 65.0%
!! TGCTGGTGGTGGTGAGTGGG+TGG + chr8_1:16469914-16469933 Msa1181840:CDS 65.0%
GGCGGATCTCCACCGACAGA+CGG - chr8_1:16470124-16470143 None:intergenic 65.0%
CGCTACCGAGTCGCGCGTTA+TGG + chr8_1:16470792-16470811 Msa1181840:intron 65.0%
!! AGCGGGTCAATAGCGGGTCG+AGG + chr8_1:16470818-16470837 Msa1181840:intron 65.0%
ATTGATCCGCCCCACTCCGC+CGG - chr8_1:16470851-16470870 None:intergenic 65.0%
AGCGGGTCGAGGCCGCTATC+CGG + chr8_1:16470829-16470848 Msa1181840:intron 70.0%
AGGCCGCTATCCGGCGGAGT+GGG + chr8_1:16470838-16470857 Msa1181840:intron 70.0%
AGCGGGTCGAGGCCGCTATC+CGG + chr8_1:16470829-16470848 Msa1181840:intron 70.0%
AGGCCGCTATCCGGCGGAGT+GGG + chr8_1:16470838-16470857 Msa1181840:intron 70.0%
GGGTCGAGGCCGCTATCCGG+CGG + chr8_1:16470832-16470851 Msa1181840:intron 75.0%
GAGGCCGCTATCCGGCGGAG+TGG + chr8_1:16470837-16470856 Msa1181840:intron 75.0%
GGCCGCTATCCGGCGGAGTG+GGG + chr8_1:16470839-16470858 Msa1181840:intron 75.0%
CGCCCCACTCCGCCGGATAG+CGG - chr8_1:16470844-16470863 None:intergenic 75.0%
CGCTATCCGGCGGAGTGGGG+CGG + chr8_1:16470842-16470861 Msa1181840:intron 75.0%
GGGTCGAGGCCGCTATCCGG+CGG + chr8_1:16470832-16470851 Msa1181840:intron 75.0%
GAGGCCGCTATCCGGCGGAG+TGG + chr8_1:16470837-16470856 Msa1181840:intron 75.0%
GGCCGCTATCCGGCGGAGTG+GGG + chr8_1:16470839-16470858 Msa1181840:intron 75.0%
CGCCCCACTCCGCCGGATAG+CGG - chr8_1:16470844-16470863 None:intergenic 75.0%
CGCTATCCGGCGGAGTGGGG+CGG + chr8_1:16470842-16470861 Msa1181840:intron 75.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 16469881 16471712 16469881 ID=Msa1181840;Name=Msa1181840
chr8_1 mRNA 16469881 16471712 16469881 ID=Msa1181840-mRNA-1;Parent=Msa1181840;Name=Msa1181840-mRNA-1;_AED=0.27;_eAED=0.27;_QI=16|0.8|0.66|0.83|0.6|0.83|6|581|92
chr8_1 exon 16469881 16469935 16469881 ID=Msa1181840-mRNA-1:exon:6157;Parent=Msa1181840-mRNA-1
chr8_1 exon 16469950 16470154 16469950 ID=Msa1181840-mRNA-1:exon:6158;Parent=Msa1181840-mRNA-1
chr8_1 exon 16470246 16470296 16470246 ID=Msa1181840-mRNA-1:exon:6159;Parent=Msa1181840-mRNA-1
chr8_1 exon 16470397 16470460 16470397 ID=Msa1181840-mRNA-1:exon:6160;Parent=Msa1181840-mRNA-1
chr8_1 exon 16471032 16471109 16471032 ID=Msa1181840-mRNA-1:exon:6161;Parent=Msa1181840-mRNA-1
chr8_1 exon 16471290 16471712 16471290 ID=Msa1181840-mRNA-1:exon:6162;Parent=Msa1181840-mRNA-1
chr8_1 five_prime_UTR 16469881 16469896 16469881 ID=Msa1181840-mRNA-1:five_prime_utr;Parent=Msa1181840-mRNA-1
chr8_1 CDS 16469897 16469935 16469897 ID=Msa1181840-mRNA-1:cds;Parent=Msa1181840-mRNA-1
chr8_1 CDS 16469950 16470154 16469950 ID=Msa1181840-mRNA-1:cds;Parent=Msa1181840-mRNA-1
chr8_1 CDS 16470246 16470280 16470246 ID=Msa1181840-mRNA-1:cds;Parent=Msa1181840-mRNA-1
chr8_1 three_prime_UTR 16470281 16470296 16470281 ID=Msa1181840-mRNA-1:three_prime_utr;Parent=Msa1181840-mRNA-1
chr8_1 three_prime_UTR 16470397 16470460 16470397 ID=Msa1181840-mRNA-1:three_prime_utr;Parent=Msa1181840-mRNA-1
chr8_1 three_prime_UTR 16471032 16471109 16471032 ID=Msa1181840-mRNA-1:three_prime_utr;Parent=Msa1181840-mRNA-1
chr8_1 three_prime_UTR 16471290 16471712 16471290 ID=Msa1181840-mRNA-1:three_prime_utr;Parent=Msa1181840-mRNA-1
Gene Sequence

>Msa1181840

ATGATTGTTGTTGCAGCTGCTGGTGGTGGTGAGTGGGTGGGGTGTGGGCTAAAATTTCCGAAAATAAGTGTTATTGGCTATATGTGTACAAGCAACAAATCCATACCCATTTTGTCCTTGGCCCATGCAGTAGTGATAGTGTTTAGCGTTGCGGCAACCCTAACAATAAAAACTCATCACACTCTCACTTTCATCCATACCGCATCTACTCCGTCTGTCGGTGGAGATCCGCCTTCGGCTTTATTTCTCAACAACACTCTTTTCGTGTGTAGATTATAA
Protein sequence

>Msa1181840

MIVVAAAGGGEWVGCGLKFPKISVIGYMCTSNKSIPILSLAHAVVIVFSVAATLTIKTHHTLTFIHTASTPSVGGDPPSALFLNNTLFVCRL*