AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1456050


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1456050 MtrunA17_Chr3g0137181 100.000 51 0 0 1 51 1 51 2.66e-32 105
Msa1456050 MtrunA17_Chr1g0149761 100.000 51 0 0 1 51 1 51 2.66e-32 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1456050 AT4G31985.1 90.196 51 5 0 1 51 1 51 1.88e-29 98.2
Msa1456050 AT3G02190.1 88.235 51 6 0 1 51 1 51 4.41e-28 94.7
Msa1456050 AT2G25210.1 90.909 44 4 0 8 51 1 44 6.60e-24 84.0

Find 40 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTTATTGTAGTTAATTAA+AGG 0.251556 tig0036842:+16412 Msa1456050:three_prime_UTR
ATAGGCTATTCATTGTGATA+TGG 0.254961 tig0036842:+16529 Msa1456050:three_prime_UTR
ATTTAAGACAATCGATGTTT+TGG 0.282559 tig0036842:+16333 Msa1456050:three_prime_UTR
GTGCAGCCGTCGAGCATTTC+CGG 0.303652 tig0036842:+15891 Msa1456050:five_prime_UTR
TTAGATTAGGGCTTCGTGTT+AGG 0.305458 tig0036842:+15848 Msa1456050:five_prime_UTR
AAGATTCTGAAATTAGATTA+GGG 0.315313 tig0036842:+15836 Msa1456050:five_prime_UTR
AAAGATTCTGAAATTAGATT+AGG 0.315654 tig0036842:+15835 Msa1456050:five_prime_UTR
ATTCGTAATCAAGAAGAAAT+TGG 0.321220 tig0036842:+16033 Msa1456050:CDS
GGGATTTGAGTTCATTCTAT+AGG 0.347390 tig0036842:+16511 Msa1456050:three_prime_UTR
AATGGCATACATCACCAATA+AGG 0.381130 tig0036842:-16645 None:intergenic
CCGGAGGCAAGGATCGAAAA+TGG 0.389081 tig0036842:+15910 Msa1456050:exon
GGCATTGTAATGTTATACTT+TGG 0.392128 tig0036842:+16354 Msa1456050:three_prime_UTR
TTATTTCATCAAGGGAAATA+CGG 0.394107 tig0036842:+16556 Msa1456050:three_prime_UTR
TAGGCTATTCATTGTGATAT+GGG 0.396854 tig0036842:+16530 Msa1456050:three_prime_UTR
TCAAGCGAGTGCAATCAAAA+TGG 0.413755 tig0036842:-16663 None:intergenic
AGAATAGACCCATCCCTTAC+TGG 0.418157 tig0036842:+16071 Msa1456050:CDS
TAGATTAGGGCTTCGTGTTA+GGG 0.422723 tig0036842:+15849 Msa1456050:five_prime_UTR
GGGTTTACAAGAAGCATTCT+CGG 0.431321 tig0036842:+15869 Msa1456050:five_prime_UTR
TCCCTTACTGGATCCGTATG+AGG 0.452674 tig0036842:+16083 Msa1456050:CDS
CACTGGCGCCGTACCAAGCT+CGG 0.478359 tig0036842:+16239 Msa1456050:CDS
AACCCTAATAGTTCAAGAAA+AGG 0.479806 tig0036842:-16293 None:intergenic
TATTTCATCAAGGGAAATAC+GGG 0.488022 tig0036842:+16557 Msa1456050:three_prime_UTR
ACAATGCTAAGCGCCGTCAC+TGG 0.497185 tig0036842:+16222 Msa1456050:CDS
CTCAAAATCCGAGCTTGGTA+CGG 0.513650 tig0036842:-16247 None:intergenic
GCTTGGTACGGCGCCAGTGA+CGG 0.518206 tig0036842:-16235 None:intergenic
TGAGGACTGACAACACCATC+AGG 0.522876 tig0036842:+16101 Msa1456050:CDS
GTCCTCATACGGATCCAGTA+AGG 0.528387 tig0036842:-16085 None:intergenic
GGACAAAACGCGGCAAAGCA+AGG 0.533748 tig0036842:-16272 None:intergenic
GTCGAGCATTTCCGGAGGCA+AGG 0.547294 tig0036842:+15899 Msa1456050:five_prime_UTR
TGGTGTTGTCAGTCCTCATA+CGG 0.570137 tig0036842:-16096 None:intergenic
AGGACCTCAAAATCCGAGCT+TGG 0.583429 tig0036842:-16252 None:intergenic
ATACGGATCCAGTAAGGGAT+GGG 0.586639 tig0036842:-16079 None:intergenic
ACAATTTCCTAGTATACAGT+AGG 0.587677 tig0036842:-16589 None:intergenic
CATACGGATCCAGTAAGGGA+TGG 0.594687 tig0036842:-16080 None:intergenic
AGAAATTGGCGAAGAAGATG+AGG 0.597939 tig0036842:+16047 Msa1456050:CDS
ATTACGAATGTCTTGTGTGA+CGG 0.598814 tig0036842:-16019 None:intergenic
TCCTCATACGGATCCAGTAA+GGG 0.602358 tig0036842:-16084 None:intergenic
TTGCCTCCGGAAATGCTCGA+CGG 0.662148 tig0036842:-15897 None:intergenic
TTCAAGAAAAGGACAAAACG+CGG 0.690502 tig0036842:-16282 None:intergenic
CAGCCGTCGAGCATTTCCGG+AGG 0.690561 tig0036842:+15894 Msa1456050:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATTTTTTCCTCTTTT+AGG + tig0036842:16198-16217 Msa1456050:intron 15.0%
!! AACTTATTGTAGTTAATTAA+AGG + tig0036842:16412-16431 Msa1456050:three_prime_UTR 15.0%
!! AAATCCGAATTAATTAAAAA+AGG - tig0036842:16444-16463 None:intergenic 15.0%
!!! TAGGTTTTATATATTTTTGT+TGG + tig0036842:16620-16639 Msa1456050:three_prime_UTR 15.0%
!!! TTTTTATTTTTTCCTCTTTT+AGG + tig0036842:16198-16217 Msa1456050:intron 15.0%
!! AACTTATTGTAGTTAATTAA+AGG + tig0036842:16412-16431 Msa1456050:three_prime_UTR 15.0%
!! AAATCCGAATTAATTAAAAA+AGG - tig0036842:16444-16463 None:intergenic 15.0%
!!! TAGGTTTTATATATTTTTGT+TGG + tig0036842:16620-16639 Msa1456050:three_prime_UTR 15.0%
!!! AAAGATTCTGAAATTAGATT+AGG + tig0036842:15835-15854 Msa1456050:five_prime_UTR 20.0%
!!! AAGATTCTGAAATTAGATTA+GGG + tig0036842:15836-15855 Msa1456050:five_prime_UTR 20.0%
!!! TATATAAGTAAGAACTTTTG+CGG + tig0036842:16380-16399 Msa1456050:three_prime_UTR 20.0%
!!! GACACCTTTTTTAATTAATT+CGG + tig0036842:16437-16456 Msa1456050:three_prime_UTR 20.0%
!!! TTATACATATTTAGTTTTGC+TGG + tig0036842:16490-16509 Msa1456050:three_prime_UTR 20.0%
!!! TATACATATTTAGTTTTGCT+GGG + tig0036842:16491-16510 Msa1456050:three_prime_UTR 20.0%
!! CAAAAATATATAAAACCTAG+AGG - tig0036842:16619-16638 None:intergenic 20.0%
!!! AAAGATTCTGAAATTAGATT+AGG + tig0036842:15835-15854 Msa1456050:five_prime_UTR 20.0%
!!! AAGATTCTGAAATTAGATTA+GGG + tig0036842:15836-15855 Msa1456050:five_prime_UTR 20.0%
!!! TATATAAGTAAGAACTTTTG+CGG + tig0036842:16380-16399 Msa1456050:three_prime_UTR 20.0%
!!! GACACCTTTTTTAATTAATT+CGG + tig0036842:16437-16456 Msa1456050:three_prime_UTR 20.0%
!!! TTATACATATTTAGTTTTGC+TGG + tig0036842:16490-16509 Msa1456050:three_prime_UTR 20.0%
!!! TATACATATTTAGTTTTGCT+GGG + tig0036842:16491-16510 Msa1456050:three_prime_UTR 20.0%
!! CAAAAATATATAAAACCTAG+AGG - tig0036842:16619-16638 None:intergenic 20.0%
! ATTCGTAATCAAGAAGAAAT+TGG + tig0036842:16033-16052 Msa1456050:CDS 25.0%
!!! ATTTAAGACAATCGATGTTT+TGG + tig0036842:16333-16352 Msa1456050:three_prime_UTR 25.0%
!!! TATGGGATTTTATTTCATCA+AGG + tig0036842:16547-16566 Msa1456050:three_prime_UTR 25.0%
!!! ATGGGATTTTATTTCATCAA+GGG + tig0036842:16548-16567 Msa1456050:three_prime_UTR 25.0%
! TTATTTCATCAAGGGAAATA+CGG + tig0036842:16556-16575 Msa1456050:three_prime_UTR 25.0%
!!! TATTTTTGTTGGATCCTTAT+TGG + tig0036842:16631-16650 Msa1456050:three_prime_UTR 25.0%
! ATTCGTAATCAAGAAGAAAT+TGG + tig0036842:16033-16052 Msa1456050:CDS 25.0%
!!! ATTTAAGACAATCGATGTTT+TGG + tig0036842:16333-16352 Msa1456050:three_prime_UTR 25.0%
!!! TATGGGATTTTATTTCATCA+AGG + tig0036842:16547-16566 Msa1456050:three_prime_UTR 25.0%
!!! ATGGGATTTTATTTCATCAA+GGG + tig0036842:16548-16567 Msa1456050:three_prime_UTR 25.0%
! TTATTTCATCAAGGGAAATA+CGG + tig0036842:16556-16575 Msa1456050:three_prime_UTR 25.0%
!!! TATTTTTGTTGGATCCTTAT+TGG + tig0036842:16631-16650 Msa1456050:three_prime_UTR 25.0%
! TGTCCTTTTCTTGAACTATT+AGG + tig0036842:16290-16309 Msa1456050:three_prime_UTR 30.0%
! GTCCTTTTCTTGAACTATTA+GGG + tig0036842:16291-16310 Msa1456050:three_prime_UTR 30.0%
AACCCTAATAGTTCAAGAAA+AGG - tig0036842:16296-16315 None:intergenic 30.0%
GGCATTGTAATGTTATACTT+TGG + tig0036842:16354-16373 Msa1456050:three_prime_UTR 30.0%
ATAGGCTATTCATTGTGATA+TGG + tig0036842:16529-16548 Msa1456050:three_prime_UTR 30.0%
TAGGCTATTCATTGTGATAT+GGG + tig0036842:16530-16549 Msa1456050:three_prime_UTR 30.0%
TATTTCATCAAGGGAAATAC+GGG + tig0036842:16557-16576 Msa1456050:three_prime_UTR 30.0%
! TTTTGTTCCTACTGTATACT+AGG + tig0036842:16582-16601 Msa1456050:three_prime_UTR 30.0%
ACAATTTCCTAGTATACAGT+AGG - tig0036842:16592-16611 None:intergenic 30.0%
!! TAGGAAATTGTTTTTCCTCT+AGG + tig0036842:16601-16620 Msa1456050:three_prime_UTR 30.0%
! TGTCCTTTTCTTGAACTATT+AGG + tig0036842:16290-16309 Msa1456050:three_prime_UTR 30.0%
! GTCCTTTTCTTGAACTATTA+GGG + tig0036842:16291-16310 Msa1456050:three_prime_UTR 30.0%
AACCCTAATAGTTCAAGAAA+AGG - tig0036842:16296-16315 None:intergenic 30.0%
GGCATTGTAATGTTATACTT+TGG + tig0036842:16354-16373 Msa1456050:three_prime_UTR 30.0%
ATAGGCTATTCATTGTGATA+TGG + tig0036842:16529-16548 Msa1456050:three_prime_UTR 30.0%
TAGGCTATTCATTGTGATAT+GGG + tig0036842:16530-16549 Msa1456050:three_prime_UTR 30.0%
TATTTCATCAAGGGAAATAC+GGG + tig0036842:16557-16576 Msa1456050:three_prime_UTR 30.0%
! TTTTGTTCCTACTGTATACT+AGG + tig0036842:16582-16601 Msa1456050:three_prime_UTR 30.0%
ACAATTTCCTAGTATACAGT+AGG - tig0036842:16592-16611 None:intergenic 30.0%
!! TAGGAAATTGTTTTTCCTCT+AGG + tig0036842:16601-16620 Msa1456050:three_prime_UTR 30.0%
ATTACGAATGTCTTGTGTGA+CGG - tig0036842:16022-16041 None:intergenic 35.0%
AATGCGAAGAAAGAGTGAAA+GGG - tig0036842:16135-16154 None:intergenic 35.0%
AAATGCGAAGAAAGAGTGAA+AGG - tig0036842:16136-16155 None:intergenic 35.0%
CTTAGCATTGTACCTAAAAG+AGG - tig0036842:16213-16232 None:intergenic 35.0%
TTCAAGAAAAGGACAAAACG+CGG - tig0036842:16285-16304 None:intergenic 35.0%
! GGGATTTGAGTTCATTCTAT+AGG + tig0036842:16511-16530 Msa1456050:three_prime_UTR 35.0%
AATGGCATACATCACCAATA+AGG - tig0036842:16648-16667 None:intergenic 35.0%
ATTACGAATGTCTTGTGTGA+CGG - tig0036842:16022-16041 None:intergenic 35.0%
AATGCGAAGAAAGAGTGAAA+GGG - tig0036842:16135-16154 None:intergenic 35.0%
AAATGCGAAGAAAGAGTGAA+AGG - tig0036842:16136-16155 None:intergenic 35.0%
CTTAGCATTGTACCTAAAAG+AGG - tig0036842:16213-16232 None:intergenic 35.0%
TTCAAGAAAAGGACAAAACG+CGG - tig0036842:16285-16304 None:intergenic 35.0%
! GGGATTTGAGTTCATTCTAT+AGG + tig0036842:16511-16530 Msa1456050:three_prime_UTR 35.0%
AATGGCATACATCACCAATA+AGG - tig0036842:16648-16667 None:intergenic 35.0%
TTAGATTAGGGCTTCGTGTT+AGG + tig0036842:15848-15867 Msa1456050:five_prime_UTR 40.0%
TAGATTAGGGCTTCGTGTTA+GGG + tig0036842:15849-15868 Msa1456050:five_prime_UTR 40.0%
!! GGGTTTACAAGAAGCATTCT+CGG + tig0036842:15869-15888 Msa1456050:five_prime_UTR 40.0%
!! GATTGAAGAGACGCTGATTT+GGG - tig0036842:15940-15959 None:intergenic 40.0%
AGCGTCTCTTCAATCGTATT+AGG + tig0036842:15945-15964 Msa1456050:intron 40.0%
GCGTCTCTTCAATCGTATTA+GGG + tig0036842:15946-15965 Msa1456050:intron 40.0%
AGAAATTGGCGAAGAAGATG+AGG + tig0036842:16047-16066 Msa1456050:CDS 40.0%
GAAAGGGAAAGAATACCTGA+TGG - tig0036842:16119-16138 None:intergenic 40.0%
! AAGAACTTTTGCGGTGTTTG+AGG + tig0036842:16389-16408 Msa1456050:three_prime_UTR 40.0%
TCAAGCGAGTGCAATCAAAA+TGG - tig0036842:16666-16685 None:intergenic 40.0%
TTAGATTAGGGCTTCGTGTT+AGG + tig0036842:15848-15867 Msa1456050:five_prime_UTR 40.0%
TAGATTAGGGCTTCGTGTTA+GGG + tig0036842:15849-15868 Msa1456050:five_prime_UTR 40.0%
!! GGGTTTACAAGAAGCATTCT+CGG + tig0036842:15869-15888 Msa1456050:five_prime_UTR 40.0%
!! GATTGAAGAGACGCTGATTT+GGG - tig0036842:15940-15959 None:intergenic 40.0%
AGCGTCTCTTCAATCGTATT+AGG + tig0036842:15945-15964 Msa1456050:intron 40.0%
GCGTCTCTTCAATCGTATTA+GGG + tig0036842:15946-15965 Msa1456050:intron 40.0%
AGAAATTGGCGAAGAAGATG+AGG + tig0036842:16047-16066 Msa1456050:CDS 40.0%
GAAAGGGAAAGAATACCTGA+TGG - tig0036842:16119-16138 None:intergenic 40.0%
! AAGAACTTTTGCGGTGTTTG+AGG + tig0036842:16389-16408 Msa1456050:three_prime_UTR 40.0%
TCAAGCGAGTGCAATCAAAA+TGG - tig0036842:16666-16685 None:intergenic 40.0%
! CGATTGAAGAGACGCTGATT+TGG - tig0036842:15941-15960 None:intergenic 45.0%
AGAATAGACCCATCCCTTAC+TGG + tig0036842:16071-16090 Msa1456050:CDS 45.0%
ATACGGATCCAGTAAGGGAT+GGG - tig0036842:16082-16101 None:intergenic 45.0%
TCCTCATACGGATCCAGTAA+GGG - tig0036842:16087-16106 None:intergenic 45.0%
!! TGGTGTTGTCAGTCCTCATA+CGG - tig0036842:16099-16118 None:intergenic 45.0%
CTCAAAATCCGAGCTTGGTA+CGG - tig0036842:16250-16269 None:intergenic 45.0%
! CGATTGAAGAGACGCTGATT+TGG - tig0036842:15941-15960 None:intergenic 45.0%
AGAATAGACCCATCCCTTAC+TGG + tig0036842:16071-16090 Msa1456050:CDS 45.0%
ATACGGATCCAGTAAGGGAT+GGG - tig0036842:16082-16101 None:intergenic 45.0%
TCCTCATACGGATCCAGTAA+GGG - tig0036842:16087-16106 None:intergenic 45.0%
!! TGGTGTTGTCAGTCCTCATA+CGG - tig0036842:16099-16118 None:intergenic 45.0%
CTCAAAATCCGAGCTTGGTA+CGG - tig0036842:16250-16269 None:intergenic 45.0%
! CCATTTTCGATCCTTGCCTC+CGG - tig0036842:15913-15932 None:intergenic 50.0%
CATACGGATCCAGTAAGGGA+TGG - tig0036842:16083-16102 None:intergenic 50.0%
TCCCTTACTGGATCCGTATG+AGG + tig0036842:16083-16102 Msa1456050:CDS 50.0%
GTCCTCATACGGATCCAGTA+AGG - tig0036842:16088-16107 None:intergenic 50.0%
TGAGGACTGACAACACCATC+AGG + tig0036842:16101-16120 Msa1456050:CDS 50.0%
! CGTACCAAGCTCGGATTTTG+AGG + tig0036842:16248-16267 Msa1456050:CDS 50.0%
AGGACCTCAAAATCCGAGCT+TGG - tig0036842:16255-16274 None:intergenic 50.0%
! CCATTTTCGATCCTTGCCTC+CGG - tig0036842:15913-15932 None:intergenic 50.0%
CATACGGATCCAGTAAGGGA+TGG - tig0036842:16083-16102 None:intergenic 50.0%
TCCCTTACTGGATCCGTATG+AGG + tig0036842:16083-16102 Msa1456050:CDS 50.0%
GTCCTCATACGGATCCAGTA+AGG - tig0036842:16088-16107 None:intergenic 50.0%
TGAGGACTGACAACACCATC+AGG + tig0036842:16101-16120 Msa1456050:CDS 50.0%
! CGTACCAAGCTCGGATTTTG+AGG + tig0036842:16248-16267 Msa1456050:CDS 50.0%
AGGACCTCAAAATCCGAGCT+TGG - tig0036842:16255-16274 None:intergenic 50.0%
TTGCCTCCGGAAATGCTCGA+CGG - tig0036842:15900-15919 None:intergenic 55.0%
CCGGAGGCAAGGATCGAAAA+TGG + tig0036842:15910-15929 Msa1456050:exon 55.0%
! ACAATGCTAAGCGCCGTCAC+TGG + tig0036842:16222-16241 Msa1456050:CDS 55.0%
GGACAAAACGCGGCAAAGCA+AGG - tig0036842:16275-16294 None:intergenic 55.0%
TTGCCTCCGGAAATGCTCGA+CGG - tig0036842:15900-15919 None:intergenic 55.0%
CCGGAGGCAAGGATCGAAAA+TGG + tig0036842:15910-15929 Msa1456050:exon 55.0%
! ACAATGCTAAGCGCCGTCAC+TGG + tig0036842:16222-16241 Msa1456050:CDS 55.0%
GGACAAAACGCGGCAAAGCA+AGG - tig0036842:16275-16294 None:intergenic 55.0%
GTGCAGCCGTCGAGCATTTC+CGG + tig0036842:15891-15910 Msa1456050:five_prime_UTR 60.0%
GTCGAGCATTTCCGGAGGCA+AGG + tig0036842:15899-15918 Msa1456050:five_prime_UTR 60.0%
GTGCAGCCGTCGAGCATTTC+CGG + tig0036842:15891-15910 Msa1456050:five_prime_UTR 60.0%
GTCGAGCATTTCCGGAGGCA+AGG + tig0036842:15899-15918 Msa1456050:five_prime_UTR 60.0%
CAGCCGTCGAGCATTTCCGG+AGG + tig0036842:15894-15913 Msa1456050:five_prime_UTR 65.0%
GCTTGGTACGGCGCCAGTGA+CGG - tig0036842:16238-16257 None:intergenic 65.0%
CACTGGCGCCGTACCAAGCT+CGG + tig0036842:16239-16258 Msa1456050:CDS 65.0%
CAGCCGTCGAGCATTTCCGG+AGG + tig0036842:15894-15913 Msa1456050:five_prime_UTR 65.0%
GCTTGGTACGGCGCCAGTGA+CGG - tig0036842:16238-16257 None:intergenic 65.0%
CACTGGCGCCGTACCAAGCT+CGG + tig0036842:16239-16258 Msa1456050:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0036842 gene 15835 16714 15835 ID=Msa1456050;Name=Msa1456050
tig0036842 mRNA 15835 16714 15835 ID=Msa1456050-mRNA-1;Parent=Msa1456050;Name=Msa1456050-mRNA-1;_AED=0.01;_eAED=0.01;_QI=94|1|1|1|1|1|3|446|51
tig0036842 exon 15835 15931 15835 ID=Msa1456050-mRNA-1:exon:20535;Parent=Msa1456050-mRNA-1
tig0036842 exon 16019 16122 16019 ID=Msa1456050-mRNA-1:exon:20536;Parent=Msa1456050-mRNA-1
tig0036842 exon 16220 16714 16220 ID=Msa1456050-mRNA-1:exon:20537;Parent=Msa1456050-mRNA-1
tig0036842 five_prime_UTR 15835 15928 15835 ID=Msa1456050-mRNA-1:five_prime_utr;Parent=Msa1456050-mRNA-1
tig0036842 CDS 15929 15931 15929 ID=Msa1456050-mRNA-1:cds;Parent=Msa1456050-mRNA-1
tig0036842 CDS 16019 16122 16019 ID=Msa1456050-mRNA-1:cds;Parent=Msa1456050-mRNA-1
tig0036842 CDS 16220 16268 16220 ID=Msa1456050-mRNA-1:cds;Parent=Msa1456050-mRNA-1
tig0036842 three_prime_UTR 16269 16714 16269 ID=Msa1456050-mRNA-1:three_prime_utr;Parent=Msa1456050-mRNA-1
Gene Sequence

>Msa1456050

ATGCCGTCACACAAGACATTCGTAATCAAGAAGAAATTGGCGAAGAAGATGAGGCAGAATAGACCCATCCCTTACTGGATCCGTATGAGGACTGACAACACCATCAGGTACAATGCTAAGCGCCGTCACTGGCGCCGTACCAAGCTCGGATTTTGA

Protein sequence

>Msa1456050

MPSHKTFVIKKKLAKKMRQNRPIPYWIRMRTDNTIRYNAKRRHWRRTKLGF*