AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1234830

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1234830 MtrunA17_Chr8g0344491 94.828 348 3 1 166 513 95 427 0.0 686
Msa1234830 MtrunA17_Chr4g0070231 68.750 352 80 5 166 513 79 404 1.08e-166 477
Msa1234830 MtrunA17_Chr3g0139111 63.934 366 96 9 166 513 93 440 1.74e-156 452
Msa1234830 MtrunA17_Chr8g0344511 90.058 171 15 1 1 169 1 171 4.73e-56 185
Msa1234830 MtrunA17_Chr2g0328791 32.270 282 162 11 168 441 54 314 2.31e-28 117
Msa1234830 MtrunA17_Chr4g0074711 30.037 273 153 8 172 439 117 356 5.02e-25 107
Msa1234830 MtrunA17_Chr2g0288251 30.182 275 151 8 172 439 103 343 4.34e-24 104
Msa1234830 MtrunA17_Chr3g0133651 30.943 265 142 9 172 430 136 365 1.17e-23 103
Msa1234830 MtrunA17_Chr4g0045221 29.368 269 160 7 172 430 159 407 1.43e-22 100
Msa1234830 MtrunA17_Chr1g0153871 29.304 273 153 9 163 430 131 368 1.77e-22 100
Msa1234830 MtrunA17_Chr3g0110211 35.971 139 82 3 159 296 59 191 3.92e-20 92.4
Msa1234830 MtrunA17_Chr6g0465751 24.632 272 152 5 163 431 59 280 1.81e-16 82.4
Msa1234830 MtrunA17_Chr6g0466501 24.632 272 152 5 163 431 59 280 4.08e-16 81.3
Msa1234830 MtrunA17_Chr4g0044541 24.903 257 140 6 163 414 68 276 7.98e-14 74.3
Msa1234830 MtrunA17_Chr3g0134141 25.431 232 120 6 172 398 67 250 9.46e-14 73.9
Msa1234830 MtrunA17_Chr4g0061451 42.857 77 44 0 220 296 44 120 9.73e-14 71.6
Msa1234830 MtrunA17_Chr1g0190211 39.189 74 45 0 218 291 132 205 3.19e-13 69.3
Msa1234830 MtrunA17_Chr7g0219021 39.241 79 48 0 220 298 42 120 4.39e-13 69.3
Msa1234830 MtrunA17_Chr3g0121631 45.333 75 40 1 220 294 38 111 5.66e-13 66.6
Msa1234830 MtrunA17_Chr3g0116631 32.824 131 80 1 172 294 152 282 1.22e-12 68.6
Msa1234830 MtrunA17_Chr3g0119311 39.024 82 50 0 220 301 38 119 9.29e-12 62.8
Msa1234830 MtrunA17_Chr3g0087481 28.873 142 91 1 163 294 94 235 9.30e-12 65.1
Msa1234830 MtrunA17_Chr7g0266751 37.662 77 48 0 218 294 32 108 1.00e-11 62.8
Msa1234830 MtrunA17_Chr1g0168601 27.612 134 95 1 163 294 96 229 2.01e-11 63.9
Msa1234830 MtrunA17_Chr6g0466451 26.374 273 144 10 168 430 53 278 2.98e-11 65.9
Msa1234830 MtrunA17_Chr6g0465631 26.374 273 144 10 168 430 53 278 3.01e-11 65.9
Msa1234830 MtrunA17_Chr3g0086861 38.961 77 47 0 220 296 46 122 6.38e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1234830 AT1G17370.1 75.499 351 65 3 166 513 87 419 0.0 531
Msa1234830 AT1G17370.2 74.929 351 64 3 166 513 87 416 0.0 527
Msa1234830 AT3G14100.1 75.568 352 66 3 166 513 92 427 0.0 525
Msa1234830 AT1G54080.1 70.904 354 74 4 166 513 96 426 5.69e-172 492
Msa1234830 AT1G54080.2 70.112 358 74 5 166 513 96 430 1.21e-169 486
Msa1234830 AT5G19350.1 30.970 268 160 9 168 430 63 310 2.11e-28 117
Msa1234830 AT5G19350.2 29.851 268 159 8 168 430 63 306 2.29e-28 117
Msa1234830 AT1G49600.1 29.699 266 153 8 172 430 162 400 1.58e-27 115
Msa1234830 AT1G49600.3 29.699 266 153 8 172 430 162 400 9.25e-27 113
Msa1234830 AT1G49600.2 29.699 266 153 8 172 430 162 400 9.25e-27 113
Msa1234830 AT4G27000.1 30.189 265 145 8 172 431 123 352 2.99e-25 108
Msa1234830 AT5G54900.1 30.566 265 144 9 172 430 103 333 1.21e-24 106
Msa1234830 AT1G47490.1 30.189 265 146 9 172 430 146 377 3.38e-24 105
Msa1234830 AT3G19130.1 27.925 265 164 8 172 430 151 394 3.54e-24 105
Msa1234830 AT1G11650.2 29.434 265 148 7 172 431 105 335 2.20e-23 102
Msa1234830 AT1G47500.1 28.467 274 157 9 163 430 139 379 1.35e-22 100
Msa1234830 AT2G18510.1 39.370 127 74 2 171 296 67 191 1.99e-20 93.2
Msa1234830 AT1G11650.1 29.362 235 124 6 172 399 105 304 9.77e-20 90.5
Msa1234830 AT4G34110.1 26.641 259 137 6 163 416 70 280 3.46e-17 85.1
Msa1234830 AT4G34110.1 27.018 285 151 10 154 430 152 387 1.95e-12 70.1
Msa1234830 AT1G47490.2 36.154 130 77 4 172 295 146 275 8.70e-17 81.6
Msa1234830 AT1G22760.1 27.132 258 136 8 163 415 83 293 1.00e-16 84.0
Msa1234830 AT3G16380.1 28.016 257 139 7 172 422 153 369 2.20e-16 82.4
Msa1234830 AT1G47500.2 34.532 139 85 4 163 295 139 277 2.89e-16 80.1
Msa1234830 AT2G23350.1 30.620 258 136 9 165 414 169 391 7.28e-15 77.8
Msa1234830 AT2G23350.1 26.800 250 130 7 172 416 89 290 5.32e-13 72.0
Msa1234830 AT1G71770.1 23.592 284 168 6 135 415 66 303 8.57e-15 77.8
Msa1234830 AT1G71770.2 23.592 284 168 6 135 415 66 303 8.57e-15 77.8
Msa1234830 AT4G24370.1 38.272 162 59 5 1 141 1 142 2.07e-14 71.6
Msa1234830 AT3G23830.2 46.753 77 41 0 220 296 37 113 2.93e-14 70.5
Msa1234830 AT3G23830.1 46.753 77 41 0 220 296 37 113 2.93e-14 70.5
Msa1234830 AT4G13860.1 45.714 70 38 0 220 289 5 74 1.29e-13 67.0
Msa1234830 AT4G24770.1 33.094 139 84 2 161 294 186 320 2.24e-13 71.6
Msa1234830 AT4G24770.2 33.094 139 84 2 161 294 186 320 2.24e-13 71.6
Msa1234830 AT2G37220.1 31.973 147 76 1 172 294 134 280 2.66e-13 70.9
Msa1234830 AT5G50250.1 32.867 143 87 2 157 294 145 283 2.66e-13 70.9
Msa1234830 AT4G13850.4 45.333 75 41 0 220 294 37 111 5.02e-13 66.6
Msa1234830 AT1G60000.1 32.540 126 82 1 172 294 128 253 8.04e-13 68.9
Msa1234830 AT4G13850.3 45.333 75 41 0 220 294 37 111 1.07e-12 66.2
Msa1234830 AT3G04500.1 33.684 95 60 1 218 309 137 231 1.17e-12 68.2
Msa1234830 AT4G13850.2 45.333 75 41 0 220 294 37 111 1.31e-12 66.2
Msa1234830 AT4G13850.1 45.333 75 41 0 220 294 37 111 1.46e-12 66.2
Msa1234830 AT4G39260.3 39.286 84 50 1 218 300 6 89 2.21e-12 63.5
Msa1234830 AT5G04280.1 43.421 76 43 0 219 294 8 83 6.54e-12 67.0
Msa1234830 AT5G61030.2 41.333 75 44 0 220 294 42 116 9.25e-12 66.6
Msa1234830 AT5G61030.1 41.333 75 44 0 220 294 42 116 9.25e-12 66.6
Msa1234830 AT1G74230.1 44.156 77 42 1 220 296 36 111 1.11e-11 66.2
Msa1234830 AT3G53460.1 45.333 75 41 0 220 294 259 333 1.96e-11 65.9
Msa1234830 AT3G53460.3 45.333 75 41 0 220 294 259 333 1.96e-11 65.9
Msa1234830 AT3G53460.2 45.333 75 41 0 220 294 259 333 1.96e-11 65.9
Msa1234830 AT3G53460.4 45.333 75 41 0 220 294 280 354 3.10e-11 65.5
Msa1234830 AT3G08000.1 38.272 81 50 0 220 300 43 123 8.10e-11 60.5
Msa1234830 AT4G39260.2 38.961 77 47 0 218 294 6 82 9.43e-11 60.1
Msa1234830 AT1G60650.2 34.314 102 67 0 220 321 14 115 9.76e-11 63.2
Msa1234830 AT1G60650.1 34.314 102 67 0 220 321 14 115 9.76e-11 63.2

Find 153 sgRNAs with CRISPR-Local

Find 300 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACCACCATTTAATTTAAA+AGG 0.167469 8_2:-12559021 None:intergenic
TACAAATGTTTAATCTAATT+TGG 0.181298 8_2:-12559058 None:intergenic
CCTATCCGCAACAGATATTT+TGG 0.201236 8_2:+12558726 Msa1234830:CDS
CATCGTCATTTCCATGCTCT+TGG 0.280996 8_2:+12558257 Msa1234830:CDS
ATCCAACGGTTGTTGAGATT+TGG 0.281240 8_2:-12554910 None:intergenic
TCTCTGTGGCAAAATAATTA+AGG 0.291025 8_2:+12558394 Msa1234830:CDS
TTCCAGATCGAGATCGAATT+CGG 0.292727 8_2:-12554859 None:intergenic
CCAATTGCAGCTTGCTTAAG+AGG 0.300686 8_2:-12558823 None:intergenic
AATTGAGAGAGAGGTTTAAG+AGG 0.303232 8_2:-12554767 None:intergenic
GGCAACCTATCAAAGTTAAC+TGG 0.303964 8_2:+12556462 Msa1234830:CDS
CCTTCCATTGAGAGTTAATA+TGG 0.304042 8_2:-12556357 None:intergenic
GTCCAGCGTGATAAAGGATT+TGG 0.304987 8_2:+12558305 Msa1234830:CDS
ATCGTCATTTCCATGCTCTT+GGG 0.313391 8_2:+12558258 Msa1234830:CDS
ACTGGGCGATCAAGGGGATT+TGG 0.334423 8_2:+12556923 Msa1234830:CDS
TCGGGATCGGGGTTAGTGTT+AGG 0.340415 8_2:-12554840 None:intergenic
ATTTGTTCAACTTCATCTTC+TGG 0.343831 8_2:-12554720 None:intergenic
GTCTGTTCCTTTCAGGTAAG+TGG 0.351721 8_2:+12557395 Msa1234830:intron
AGCTGCAATTGGAGCAAGCC+AGG 0.365517 8_2:+12558834 Msa1234830:CDS
CCTCTTAAGCAAGCTGCAAT+TGG 0.368129 8_2:+12558823 Msa1234830:CDS
CACCAACAAATATGTTATAA+TGG 0.369265 8_2:-12556609 None:intergenic
TATGCCCAGTTAACTTTGAT+AGG 0.370081 8_2:-12556467 None:intergenic
TTGGGAATTGTTGAGGTAGA+AGG 0.374094 8_2:-12554810 None:intergenic
GTTCGTGTCCAGCGTGATAA+AGG 0.374267 8_2:+12558299 Msa1234830:CDS
AGAGGATTTGAAGCAGTTCC+AGG 0.379667 8_2:-12558673 None:intergenic
GTAGACAAATACGCTGCAAC+TGG 0.382099 8_2:+12557425 Msa1234830:CDS
TACTCATGCTGAGGCAGCTC+TGG 0.384996 8_2:+12558343 Msa1234830:CDS
CCTGCTGCTGCGCCTCTACC+AGG 0.385593 8_2:+12558703 Msa1234830:CDS
CATTTCCATGCTCTTGGGGC+TGG 0.386711 8_2:+12558263 Msa1234830:CDS
GCTGTGCAGCAGCAACATTT+TGG 0.387873 8_2:-12558876 None:intergenic
AGATTCGGAAATTGATTGAA+TGG 0.391935 8_2:+12555048 Msa1234830:CDS
GGCCATTATAACATATTTGT+TGG 0.398953 8_2:+12556607 Msa1234830:intron
CCTGGTAGAGGCGCAGCAGC+AGG 0.402401 8_2:-12558703 None:intergenic
TGTTCAAATATAGTGCTCTT+GGG 0.408510 8_2:+12558633 Msa1234830:intron
ATAACAACAATAAGATATAT+AGG 0.409012 8_2:-12559223 None:intergenic
TCGGGGTTAGTGTTAGGATT+GGG 0.411598 8_2:-12554834 None:intergenic
GTGTTCAAATATAGTGCTCT+TGG 0.414047 8_2:+12558632 Msa1234830:intron
CTACATCGGAAACTCAATTT+CGG 0.415949 8_2:+12554991 Msa1234830:CDS
AGGTGAAGGACGAGATAGAT+GGG 0.418413 8_2:-12554790 None:intergenic
GATTCGGAAATTGATTGAAT+GGG 0.423037 8_2:+12555049 Msa1234830:CDS
GACAGTGATGCCGGCAGGAT+TGG 0.423535 8_2:-12554967 None:intergenic
TTCCTTTCAGGTAAGTGGCT+TGG 0.425737 8_2:+12557400 Msa1234830:intron
TCGAGATCGAATTCGGGATC+GGG 0.426436 8_2:-12554852 None:intergenic
CGATCGCAGATCTGCTGCTC+TGG 0.428701 8_2:+12556334 Msa1234830:CDS
AGAGGCGCAGCAGCAGGTGG+GGG 0.429003 8_2:-12558697 None:intergenic
GAACTAACAAATGGTTCATC+TGG 0.429816 8_2:+12557508 Msa1234830:CDS
TCCAGCGTGATAAAGGATTT+GGG 0.430462 8_2:+12558306 Msa1234830:CDS
TAGTAACAATAAAACCCAAA+TGG 0.431858 8_2:-12559166 None:intergenic
GGTAGAGGCGCAGCAGCAGG+TGG 0.433312 8_2:-12558700 None:intergenic
ATCAGAAGACTGGGCGATCA+AGG 0.435048 8_2:+12556915 Msa1234830:CDS
CGCTGCAACTGGGCAACAAA+AGG 0.437840 8_2:+12557436 Msa1234830:CDS
CTATTATTCTGCACATTGGT+TGG 0.444186 8_2:+12558960 Msa1234830:three_prime_UTR
TCCAGATCGAGATCGAATTC+GGG 0.460052 8_2:-12554858 None:intergenic
TTAGTGTTAGGATTGGGATT+GGG 0.463378 8_2:-12554828 None:intergenic
GGGAGCAAGCCCACTCCACC+TGG 0.463873 8_2:+12558655 Msa1234830:CDS
ACTGTTTATGTGGGTAACCT+TGG 0.473526 8_2:+12557848 Msa1234830:CDS
TCAGTACACAACTGTTTATG+TGG 0.476167 8_2:+12557838 Msa1234830:CDS
GGTGTTAAAAGTAAAACTCT+AGG 0.478246 8_2:+12558981 Msa1234830:three_prime_UTR
CTGGCCATATTAACTCTCAA+TGG 0.478563 8_2:+12556353 Msa1234830:CDS
GTGTACTGAGGATTGTTCTC+AGG 0.482516 8_2:-12557824 None:intergenic
TGTTTAATCTAATTTGGTAG+TGG 0.485287 8_2:-12559052 None:intergenic
ATCGGGGTTAGTGTTAGGAT+TGG 0.486040 8_2:-12554835 None:intergenic
GTTATGTGGGATCAGAAGAC+TGG 0.486448 8_2:+12556905 Msa1234830:CDS
AAGGTGAAGGACGAGATAGA+TGG 0.487598 8_2:-12554791 None:intergenic
AGTAACAATAAAACCCAAAT+GGG 0.490147 8_2:-12559165 None:intergenic
TCCCGAATTCGATCTCGATC+TGG 0.492294 8_2:+12554857 Msa1234830:CDS
AAGATGAAGATGTTCAGATT+CGG 0.494104 8_2:+12555033 Msa1234830:CDS
ATGAAGCTTACGAACAACTT+TGG 0.494255 8_2:-12556684 None:intergenic
GGTCAGAGAGAGGATACATC+AGG 0.501078 8_2:+12556497 Msa1234830:CDS
AACTGGGCAACAAAAGGTGC+TGG 0.502538 8_2:+12557442 Msa1234830:CDS
GGCAGCTCTGGCAATTCAGA+TGG 0.502691 8_2:+12558355 Msa1234830:CDS
TCTTGGGGCTGGTGTGATAG+AGG 0.507053 8_2:+12558274 Msa1234830:CDS
ATCGAGATCGAATTCGGGAT+CGG 0.507649 8_2:-12554853 None:intergenic
ATAGATGGGAATTGAGAGAG+AGG 0.508905 8_2:-12554776 None:intergenic
GTTAGTGTTAGGATTGGGAT+TGG 0.512563 8_2:-12554829 None:intergenic
CCAAAATATCTGTTGCGGAT+AGG 0.514090 8_2:-12558726 None:intergenic
CAAGCCAGGCGATATACGAC+GGG 0.515042 8_2:+12558848 Msa1234830:CDS
TGTTTCATTCCGTAGCCAGC+AGG 0.516705 8_2:+12556949 Msa1234830:CDS
ATATCTGTTGCGGATAGGCC+TGG 0.519370 8_2:-12558721 None:intergenic
TAGAGATGCAAGAGTTATGT+GGG 0.520268 8_2:+12556892 Msa1234830:intron
CGATGTAGAGACAGTGATGC+CGG 0.521504 8_2:-12554976 None:intergenic
TTAGAGATGCAAGAGTTATG+TGG 0.526961 8_2:+12556891 Msa1234830:intron
TCAGAAGACTGGGCGATCAA+GGG 0.527570 8_2:+12556916 Msa1234830:CDS
ATTGGGATTGGGAATTGTTG+AGG 0.528159 8_2:-12554817 None:intergenic
TAGAGGCGCAGCAGCAGGTG+GGG 0.531472 8_2:-12558698 None:intergenic
CAGCTAGCAATGAGCAAGAT+GGG 0.532413 8_2:+12558760 Msa1234830:CDS
GAAGCAGTTCCAGGTGGAGT+GGG 0.535669 8_2:-12558664 None:intergenic
ACAGACGGCGACGGAGGAGA+AGG 0.542208 8_2:-12555095 None:intergenic
GCAGCTAGCAATGAGCAAGA+TGG 0.547662 8_2:+12558759 Msa1234830:CDS
TCGTCATTTCCATGCTCTTG+GGG 0.548558 8_2:+12558259 Msa1234830:CDS
ATCCAAATCTCAACAACCGT+TGG 0.549005 8_2:+12554908 Msa1234830:CDS
TGAAGCAGTTCCAGGTGGAG+TGG 0.549578 8_2:-12558665 None:intergenic
GTCGTCAGATACATCGGAAG+AGG 0.549618 8_2:-12555144 None:intergenic
GTTCAAATATAGTGCTCTTG+GGG 0.552630 8_2:+12558634 Msa1234830:intron
AGTGTGGTGGAACTAACAAA+TGG 0.554047 8_2:+12557499 Msa1234830:CDS
GTAGAGGCGCAGCAGCAGGT+GGG 0.554923 8_2:-12558699 None:intergenic
GTTGCGGATAGGCCTGGTAG+AGG 0.555251 8_2:-12558715 None:intergenic
GTAGAGACAGTGATGCCGGC+AGG 0.557002 8_2:-12554972 None:intergenic
TGTATCCTCTCTCTGACCAC+TGG 0.563870 8_2:-12556492 None:intergenic
TTAAGAGGATGCTGTGCTTG+AGG 0.565759 8_2:-12558808 None:intergenic
TCACACCAGCCCCAAGAGCA+TGG 0.567987 8_2:-12558268 None:intergenic
ATATCTATTATTCTGCACAT+TGG 0.568864 8_2:+12558956 Msa1234830:three_prime_UTR
TAAGAGGATTTCGTCTTGTG+CGG 0.573418 8_2:-12554751 None:intergenic
TGTTGGTGACCTCAGTCCTG+AGG 0.574357 8_2:+12556624 Msa1234830:CDS
CAGAAGACTGGGCGATCAAG+GGG 0.575925 8_2:+12556917 Msa1234830:CDS
TTATGTGGGTAACCTTGGAT+CGG 0.577937 8_2:+12557853 Msa1234830:CDS
AAAACTAAATCCAATCCTGC+CGG 0.579485 8_2:+12554957 Msa1234830:CDS
CCATATTAACTCTCAATGGA+AGG 0.584606 8_2:+12556357 Msa1234830:CDS
AGTGCTATAAATGATTTGAC+CGG 0.585401 8_2:+12557067 Msa1234830:CDS
GCATGCGAAAAGTGTAGCAT+CGG 0.586459 8_2:-12556650 None:intergenic
AGTTTCTTACCTCCGATCCA+AGG 0.587164 8_2:-12557865 None:intergenic
AGTGTAGCATCGGTAACCTC+AGG 0.587772 8_2:-12556640 None:intergenic
TGTGGGTAACCTTGGATCGG+AGG 0.587914 8_2:+12557856 Msa1234830:CDS
ACCCAAATCCTTTATCACGC+TGG 0.596264 8_2:-12558307 None:intergenic
GTGTTAAAAGTAAAACTCTA+GGG 0.603841 8_2:+12558982 Msa1234830:three_prime_UTR
GCAAGCCAGGCGATATACGA+CGG 0.611397 8_2:+12558847 Msa1234830:CDS
GCAGCTCTGGCAATTCAGAT+GGG 0.613986 8_2:+12558356 Msa1234830:CDS
ATTGTTGAGGTAGAAGGTGA+AGG 0.614493 8_2:-12554804 None:intergenic
CGAGATCGAATTCGGGATCG+GGG 0.616552 8_2:-12554851 None:intergenic
TTTGCCACAGAGATAAGACT+GGG 0.619779 8_2:-12558384 None:intergenic
GGATTTGAAGCAGTTCCAGG+TGG 0.620256 8_2:-12558670 None:intergenic
ATCGTCGTCGTCAGATACAT+CGG 0.621613 8_2:-12555150 None:intergenic
GTATGCCAAAATATCTGTTG+CGG 0.625088 8_2:-12558731 None:intergenic
TAGACAAATACGCTGCAACT+GGG 0.625372 8_2:+12557426 Msa1234830:CDS
GCAACCTATCAAAGTTAACT+GGG 0.627335 8_2:+12556463 Msa1234830:CDS
TCGTCAGATACATCGGAAGA+GGG 0.627438 8_2:-12555143 None:intergenic
CAAGAGCATGGAAATGACGA+TGG 0.630427 8_2:-12558256 None:intergenic
TTATGTGGGATCAGAAGACT+GGG 0.632262 8_2:+12556906 Msa1234830:CDS
CTCGGACAGTAAAAGTGTGG+TGG 0.632747 8_2:+12557486 Msa1234830:CDS
AGGATCGTGATCTTCATCAT+CGG 0.635603 8_2:-12555120 None:intergenic
TATTGAAGAGAAGCAAAACT+CGG 0.637066 8_2:+12557468 Msa1234830:CDS
ATTTCGGTATGAAAGTGAAG+AGG 0.639281 8_2:+12555007 Msa1234830:CDS
CGGCATCACTGTCTCTACAT+CGG 0.640850 8_2:+12554977 Msa1234830:CDS
ATCTCTTTACCGTCCTCTGT+AGG 0.641602 8_2:-12557785 None:intergenic
CAGTACACAACTGTTTATGT+GGG 0.642164 8_2:+12557839 Msa1234830:CDS
ATATGCCAGTGGTCAGAGAG+AGG 0.645760 8_2:+12556487 Msa1234830:CDS
TCGGATACAGACGGCGACGG+AGG 0.652745 8_2:-12555101 None:intergenic
ATGAGTTTGTATCATGTATG+CGG 0.659413 8_2:+12559080 Msa1234830:three_prime_UTR
GAGATATAGTACTCATGCTG+AGG 0.660148 8_2:+12558334 Msa1234830:CDS
CTAGCAATGAGCAAGATGGG+TGG 0.664649 8_2:+12558763 Msa1234830:CDS
TGGGCAACAAAAGGTGCTGG+CGG 0.665006 8_2:+12557445 Msa1234830:CDS
TTCAAATATAGTGCTCTTGG+GGG 0.666129 8_2:+12558635 Msa1234830:intron
TCATCGGATACAGACGGCGA+CGG 0.666925 8_2:-12555104 None:intergenic
AATCTGGCGTTGATATCCAA+CGG 0.669753 8_2:-12554924 None:intergenic
TCAGATACATCGGAAGAGGG+AGG 0.674537 8_2:-12555140 None:intergenic
AAACTCGGACAGTAAAAGTG+TGG 0.685436 8_2:+12557483 Msa1234830:CDS
CATCGGTAACCTCAGGACTG+AGG 0.688396 8_2:-12556633 None:intergenic
GCATCTGTGAAAATGAGTCG+AGG 0.689675 8_2:+12554675 None:intergenic
AGCCAGGCGATATACGACGG+GGG 0.690976 8_2:+12558850 Msa1234830:CDS
GTTAACTGGGCATATGCCAG+TGG 0.699312 8_2:+12556476 Msa1234830:CDS
AATGCCCAGTCTTATCTCTG+TGG 0.700945 8_2:+12558380 Msa1234830:CDS
ACATAAACAGTTGTGTACTG+AGG 0.705415 8_2:-12557836 None:intergenic
TCTTCATCATCGGATACAGA+CGG 0.706044 8_2:-12555110 None:intergenic
AAGCCAGGCGATATACGACG+GGG 0.734249 8_2:+12558849 Msa1234830:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATAAAATTATTTTTTGAC+TGG - chr8_2:12555194-12555213 None:intergenic 10.0%
!!! CTTTTAAAATAATTAAACAT+AGG - chr8_2:12557254-12557273 None:intergenic 10.0%
!!! AAATGTTTGATTTTTAATAC+TGG + chr8_2:12555310-12555329 Msa1234830:intron 15.0%
!! GTTTAAATTATTATTTGTGT+CGG + chr8_2:12555482-12555501 Msa1234830:intron 15.0%
!!! ATGTTTTGAACAAATAATTT+TGG + chr8_2:12556548-12556567 Msa1234830:intron 15.0%
!!! TATTTTTACTAAGAAATCAT+AGG - chr8_2:12556713-12556732 None:intergenic 15.0%
!!! TAAAATCTTAATTTGTGTTT+TGG + chr8_2:12556985-12557004 Msa1234830:intron 15.0%
!!! AAAATCTTAATTTGTGTTTT+GGG + chr8_2:12556986-12557005 Msa1234830:intron 15.0%
!!! TATATTTTTTGTCATTTTGT+AGG + chr8_2:12557036-12557055 Msa1234830:intron 15.0%
!! TAATTGGCTAAAATAAAATA+AGG - chr8_2:12557119-12557138 None:intergenic 15.0%
!!! TATTTTAGCCAATTATAATT+TGG + chr8_2:12557124-12557143 Msa1234830:intron 15.0%
!! TATATCTATTAATCAATCTA+TGG + chr8_2:12557893-12557912 Msa1234830:intron 15.0%
!! ACATATATTGTAAAATCAAA+AGG - chr8_2:12558127-12558146 None:intergenic 15.0%
!!! CTTTTTCCTTTTAAATTAAA+TGG + chr8_2:12559015-12559034 Msa1234830:three_prime_UTR 15.0%
!! TACAAATGTTTAATCTAATT+TGG - chr8_2:12559061-12559080 None:intergenic 15.0%
!! ATAACAACAATAAGATATAT+AGG - chr8_2:12559226-12559245 None:intergenic 15.0%
!! TGTATAAATCACTAGTAAAA+GGG - chr8_2:12555265-12555284 None:intergenic 20.0%
!! ATGTATAAATCACTAGTAAA+AGG - chr8_2:12555266-12555285 None:intergenic 20.0%
!! TTTAATTAATCACCTTCAAT+AGG + chr8_2:12555838-12555857 Msa1234830:intron 20.0%
!! AGTATGTGTTATATATTCTA+TGG - chr8_2:12556075-12556094 None:intergenic 20.0%
!!! ATATGTTTCTTTGAATTTTC+AGG + chr8_2:12556586-12556605 Msa1234830:intron 20.0%
!!! TAGCATCCATTTTATATATT+TGG + chr8_2:12556731-12556750 Msa1234830:intron 20.0%
!! CCAATTCCAAATATATAAAA+TGG - chr8_2:12556740-12556759 None:intergenic 20.0%
!!! CCATTTTATATATTTGGAAT+TGG + chr8_2:12556737-12556756 Msa1234830:intron 20.0%
!! CAATCTTCATTTATTCAAAA+TGG + chr8_2:12556762-12556781 Msa1234830:intron 20.0%
!! TTATTCCATATATGTGATAA+TGG + chr8_2:12556800-12556819 Msa1234830:intron 20.0%
!!! CACTAACTTTATCTTTTTTA+TGG + chr8_2:12556866-12556885 Msa1234830:intron 20.0%
!!! AAATCTTAATTTGTGTTTTG+GGG + chr8_2:12556987-12557006 Msa1234830:intron 20.0%
!! ATTTACTAACGAAAAATCTA+TGG - chr8_2:12557158-12557177 None:intergenic 20.0%
!! GCTGAAGAAAAAAAATATAT+AGG - chr8_2:12557294-12557313 None:intergenic 20.0%
!! TATTATTGTTTAAGGAACTA+TGG - chr8_2:12557547-12557566 None:intergenic 20.0%
!! TTCCTTAAACAATAATAGAA+TGG + chr8_2:12557550-12557569 Msa1234830:intron 20.0%
!! AACCATTCTATTATTGTTTA+AGG - chr8_2:12557555-12557574 None:intergenic 20.0%
!! TCTTGATTTAAAAATAGATC+CGG + chr8_2:12557611-12557630 Msa1234830:intron 20.0%
!! AATAAACAAAATGCTAAAAC+AGG - chr8_2:12558042-12558061 None:intergenic 20.0%
!! TTAATACATCTCTAACATAA+TGG - chr8_2:12558082-12558101 None:intergenic 20.0%
!! TATATTGTAAAATCAAAAGG+TGG - chr8_2:12558124-12558143 None:intergenic 20.0%
!! CTTTATTCATGAATTTGATT+TGG + chr8_2:12558581-12558600 Msa1234830:intron 20.0%
!!! TTTCCTTTTAAATTAAATGG+TGG + chr8_2:12559018-12559037 Msa1234830:three_prime_UTR 20.0%
!!! ATTATTTTTTGACTGGTTTG+CGG - chr8_2:12555187-12555206 None:intergenic 25.0%
!! TTTTCGCAATAATTGTTACT+GGG + chr8_2:12555219-12555238 Msa1234830:intron 25.0%
! GTATAAATCACTAGTAAAAG+GGG - chr8_2:12555264-12555283 None:intergenic 25.0%
!! AGAGAATAATTTTCATGCAA+GGG - chr8_2:12555369-12555388 None:intergenic 25.0%
! TTGTAACAGTGAAAATTACA+TGG + chr8_2:12555535-12555554 Msa1234830:intron 25.0%
! TAGTATGTTTCGCTTAATAT+AGG - chr8_2:12555810-12555829 None:intergenic 25.0%
!!! TTCATTGTTTTACCAATTCT+CGG - chr8_2:12555990-12556009 None:intergenic 25.0%
! GTTATATATTCTATGGTTGA+TGG - chr8_2:12556068-12556087 None:intergenic 25.0%
! AGTTCTTCTATAATTGTGTT+AGG + chr8_2:12556393-12556412 Msa1234830:intron 25.0%
!! ATAATTGTGTTAGGTTAGTT+TGG + chr8_2:12556402-12556421 Msa1234830:intron 25.0%
!! AATTCAATGGTTTGTAGATT+CGG + chr8_2:12556440-12556459 Msa1234830:intron 25.0%
! CACCAACAAATATGTTATAA+TGG - chr8_2:12556612-12556631 None:intergenic 25.0%
!! AGGCTTTTCCAAATTATAAT+TGG - chr8_2:12557135-12557154 None:intergenic 25.0%
!! ATTATTTTCACTTATGATCG+AGG + chr8_2:12557358-12557377 Msa1234830:intron 25.0%
! CTTGATTTAAAAATAGATCC+GGG + chr8_2:12557612-12557631 Msa1234830:intron 25.0%
! TTGATTTAAAAATAGATCCG+GGG + chr8_2:12557613-12557632 Msa1234830:intron 25.0%
! TTAACCACCTAAAAATCATT+AGG - chr8_2:12557676-12557695 None:intergenic 25.0%
!! CTGTTTTCTTAATTAACAGA+TGG + chr8_2:12557728-12557747 Msa1234830:intron 25.0%
!!! AAACATTTTTTATCCTACAG+AGG + chr8_2:12557772-12557791 Msa1234830:CDS 25.0%
! GCATCATAAAATAATAAGCT+TGG - chr8_2:12557963-12557982 None:intergenic 25.0%
!! AGGAATACAAATATTGGTTA+TGG - chr8_2:12558016-12558035 None:intergenic 25.0%
! TTTATTCTCCATTTCTACTT+TGG + chr8_2:12558056-12558075 Msa1234830:intron 25.0%
! AAAGGTCGTAATGATTAAAA+TGG - chr8_2:12558206-12558225 None:intergenic 25.0%
!!! ATTTTAATCATTACGACCTT+TGG + chr8_2:12558205-12558224 Msa1234830:intron 25.0%
! ATATCTATTATTCTGCACAT+TGG + chr8_2:12558956-12558975 Msa1234830:three_prime_UTR 25.0%
! GTGTTAAAAGTAAAACTCTA+GGG + chr8_2:12558982-12559001 Msa1234830:three_prime_UTR 25.0%
!! TCACCACCATTTAATTTAAA+AGG - chr8_2:12559024-12559043 None:intergenic 25.0%
!!! TTTTAAATTAAATGGTGGTG+AGG + chr8_2:12559023-12559042 Msa1234830:three_prime_UTR 25.0%
! TGTTTAATCTAATTTGGTAG+TGG - chr8_2:12559055-12559074 None:intergenic 25.0%
! AGTAACAATAAAACCCAAAT+GGG - chr8_2:12559168-12559187 None:intergenic 25.0%
! TAGTAACAATAAAACCCAAA+TGG - chr8_2:12559169-12559188 None:intergenic 25.0%
ATTTGTTCAACTTCATCTTC+TGG - chr8_2:12554723-12554742 None:intergenic 30.0%
AAGATGAAGATGTTCAGATT+CGG + chr8_2:12555033-12555052 Msa1234830:CDS 30.0%
AGATTCGGAAATTGATTGAA+TGG + chr8_2:12555048-12555067 Msa1234830:CDS 30.0%
GATTCGGAAATTGATTGAAT+GGG + chr8_2:12555049-12555068 Msa1234830:CDS 30.0%
! CTTTTCGCAATAATTGTTAC+TGG + chr8_2:12555218-12555237 Msa1234830:intron 30.0%
! CAGAGAATAATTTTCATGCA+AGG - chr8_2:12555370-12555389 None:intergenic 30.0%
AATTATTCTCTGACACTTCT+TGG + chr8_2:12555378-12555397 Msa1234830:intron 30.0%
TTACATGGATGAAAAGAAGA+TGG + chr8_2:12555550-12555569 Msa1234830:intron 30.0%
AGCTATCATGAATTCTTTGT+TGG + chr8_2:12555601-12555620 Msa1234830:intron 30.0%
TCATGAATTCTTTGTTGGAA+TGG + chr8_2:12555606-12555625 Msa1234830:intron 30.0%
TGTTCAAAGTAATTGAACTG+AGG + chr8_2:12555673-12555692 Msa1234830:intron 30.0%
GAACTGAGGTGATTAAATTT+AGG + chr8_2:12555687-12555706 Msa1234830:intron 30.0%
TAAGACTAGCAATCTTGATA+AGG - chr8_2:12555752-12555771 None:intergenic 30.0%
!! TATCAAGATTGCTAGTCTTA+TGG + chr8_2:12555752-12555771 Msa1234830:intron 30.0%
CATGCTAATTAATTACCCTT+TGG - chr8_2:12555864-12555883 None:intergenic 30.0%
TTACACTCAGTTCAATTCAA+TGG + chr8_2:12556427-12556446 Msa1234830:intron 30.0%
! ATTCAATGGTTTGTAGATTC+GGG + chr8_2:12556441-12556460 Msa1234830:intron 30.0%
GGCCATTATAACATATTTGT+TGG + chr8_2:12556607-12556626 Msa1234830:intron 30.0%
ATGCACCATTATCACATATA+TGG - chr8_2:12556808-12556827 None:intergenic 30.0%
TGCATACTAAACAACTTTGT+TGG + chr8_2:12556828-12556847 Msa1234830:intron 30.0%
AGTGCTATAAATGATTTGAC+CGG + chr8_2:12557067-12557086 Msa1234830:CDS 30.0%
TACTAACGAAAAATCTATGG+AGG - chr8_2:12557155-12557174 None:intergenic 30.0%
!!! CTGAGAATATGGTTTTGTTT+CGG - chr8_2:12557182-12557201 None:intergenic 30.0%
AAAACCATATTCTCAGTTCA+AGG + chr8_2:12557186-12557205 Msa1234830:intron 30.0%
AAACCATATTCTCAGTTCAA+GGG + chr8_2:12557187-12557206 Msa1234830:intron 30.0%
TATTGAAGAGAAGCAAAACT+CGG + chr8_2:12557468-12557487 Msa1234830:CDS 30.0%
TGATTTAAAAATAGATCCGG+GGG + chr8_2:12557614-12557633 Msa1234830:intron 30.0%
CCTAAAAATCATTAGGTCAA+GGG - chr8_2:12557669-12557688 None:intergenic 30.0%
ACCTAAAAATCATTAGGTCA+AGG - chr8_2:12557670-12557689 None:intergenic 30.0%
!! TTGACCTAATGATTTTTAGG+TGG + chr8_2:12557669-12557688 Msa1234830:intron 30.0%
!!! ATTTTTTATCCTACAGAGGA+CGG + chr8_2:12557776-12557795 Msa1234830:CDS 30.0%
TAAGCTTGGAAAATCATCAA+TGG - chr8_2:12557949-12557968 None:intergenic 30.0%
!!! AGTAATGTTTTGAAACCCAT+TGG + chr8_2:12557989-12558008 Msa1234830:intron 30.0%
! AATATTGGTTATGGACCAAT+GGG - chr8_2:12558007-12558026 None:intergenic 30.0%
! AAATATTGGTTATGGACCAA+TGG - chr8_2:12558008-12558027 None:intergenic 30.0%
AGGTGAAGGAATACAAATAT+TGG - chr8_2:12558022-12558041 None:intergenic 30.0%
TCTCTGTGGCAAAATAATTA+AGG + chr8_2:12558394-12558413 Msa1234830:CDS 30.0%
TTATGCTGAATATTGTGTGA+AGG + chr8_2:12558485-12558504 Msa1234830:intron 30.0%
TTGAACACAATAATAGTCAG+AGG - chr8_2:12558620-12558639 None:intergenic 30.0%
TGTTCAAATATAGTGCTCTT+GGG + chr8_2:12558633-12558652 Msa1234830:intron 30.0%
GGTGTTAAAAGTAAAACTCT+AGG + chr8_2:12558981-12559000 Msa1234830:three_prime_UTR 30.0%
ATGAGTTTGTATCATGTATG+CGG + chr8_2:12559080-12559099 Msa1234830:three_prime_UTR 30.0%
AATTGAGAGAGAGGTTTAAG+AGG - chr8_2:12554770-12554789 None:intergenic 35.0%
TTAGTGTTAGGATTGGGATT+GGG - chr8_2:12554831-12554850 None:intergenic 35.0%
AAAACTAAATCCAATCCTGC+CGG + chr8_2:12554957-12554976 Msa1234830:CDS 35.0%
CTACATCGGAAACTCAATTT+CGG + chr8_2:12554991-12555010 Msa1234830:CDS 35.0%
ATTTCGGTATGAAAGTGAAG+AGG + chr8_2:12555007-12555026 Msa1234830:CDS 35.0%
ATTATGCTAGACATGTCGTT+AGG + chr8_2:12555401-12555420 Msa1234830:intron 35.0%
TCAATGTATCACATGTGTGT+CGG - chr8_2:12555450-12555469 None:intergenic 35.0%
ATGGATGAAAAGAAGATGGA+AGG + chr8_2:12555554-12555573 Msa1234830:intron 35.0%
ATGGAAGGTTTATGTTGTTG+TGG + chr8_2:12555569-12555588 Msa1234830:intron 35.0%
!!! AGTTTTGAGTTGTGCTTTGA+TGG + chr8_2:12555920-12555939 Msa1234830:intron 35.0%
! TAAGGAAGTTGCTTTGACTA+TGG + chr8_2:12555952-12555971 Msa1234830:intron 35.0%
! TCTCTTTTGCAGTCATCTTA+TGG + chr8_2:12556296-12556315 Msa1234830:intron 35.0%
CCTTCCATTGAGAGTTAATA+TGG - chr8_2:12556360-12556379 None:intergenic 35.0%
CCATATTAACTCTCAATGGA+AGG + chr8_2:12556357-12556376 Msa1234830:CDS 35.0%
GCAACCTATCAAAGTTAACT+GGG + chr8_2:12556463-12556482 Msa1234830:CDS 35.0%
TATGCCCAGTTAACTTTGAT+AGG - chr8_2:12556470-12556489 None:intergenic 35.0%
ATGAAGCTTACGAACAACTT+TGG - chr8_2:12556687-12556706 None:intergenic 35.0%
!! AAACAACTTTGTTGGTGACT+TGG + chr8_2:12556836-12556855 Msa1234830:intron 35.0%
TTAGAGATGCAAGAGTTATG+TGG + chr8_2:12556891-12556910 Msa1234830:intron 35.0%
TAGAGATGCAAGAGTTATGT+GGG + chr8_2:12556892-12556911 Msa1234830:intron 35.0%
TAAGACAAAAATAGACCTGC+TGG - chr8_2:12556967-12556986 None:intergenic 35.0%
GTCAATGACAAGTTTCTTAC+CGG - chr8_2:12557089-12557108 None:intergenic 35.0%
GAACTAACAAATGGTTCATC+TGG + chr8_2:12557508-12557527 Msa1234830:CDS 35.0%
ATTAGGTCAAGGGTAAAAAG+AGG - chr8_2:12557659-12557678 None:intergenic 35.0%
! CCCTTGACCTAATGATTTTT+AGG + chr8_2:12557666-12557685 Msa1234830:intron 35.0%
ACATAAACAGTTGTGTACTG+AGG - chr8_2:12557839-12557858 None:intergenic 35.0%
TCAGTACACAACTGTTTATG+TGG + chr8_2:12557838-12557857 Msa1234830:CDS 35.0%
CAGTACACAACTGTTTATGT+GGG + chr8_2:12557839-12557858 Msa1234830:CDS 35.0%
CAAAATGCTAAAACAGGTGA+AGG - chr8_2:12558036-12558055 None:intergenic 35.0%
CATAATGGCCAAAGTAGAAA+TGG - chr8_2:12558067-12558086 None:intergenic 35.0%
!! CTGTATCTTTGAAATCTGTG+CGG + chr8_2:12558168-12558187 Msa1234830:intron 35.0%
AAGCATGTCAATTGTCAATG+TGG - chr8_2:12558524-12558543 None:intergenic 35.0%
ATAGTCAGAGGCAATAAGAT+CGG - chr8_2:12558608-12558627 None:intergenic 35.0%
GTGTTCAAATATAGTGCTCT+TGG + chr8_2:12558632-12558651 Msa1234830:intron 35.0%
GTTCAAATATAGTGCTCTTG+GGG + chr8_2:12558634-12558653 Msa1234830:intron 35.0%
TTCAAATATAGTGCTCTTGG+GGG + chr8_2:12558635-12558654 Msa1234830:intron 35.0%
GTATGCCAAAATATCTGTTG+CGG - chr8_2:12558734-12558753 None:intergenic 35.0%
CTATTATTCTGCACATTGGT+TGG + chr8_2:12558960-12558979 Msa1234830:three_prime_UTR 35.0%
! ATGTTTTCATCTCACCCATT+TGG + chr8_2:12559151-12559170 Msa1234830:three_prime_UTR 35.0%
! TGTTTTCATCTCACCCATTT+GGG + chr8_2:12559152-12559171 Msa1234830:three_prime_UTR 35.0%
TAAGAGGATTTCGTCTTGTG+CGG - chr8_2:12554754-12554773 None:intergenic 40.0%
ATAGATGGGAATTGAGAGAG+AGG - chr8_2:12554779-12554798 None:intergenic 40.0%
ATTGTTGAGGTAGAAGGTGA+AGG - chr8_2:12554807-12554826 None:intergenic 40.0%
TTGGGAATTGTTGAGGTAGA+AGG - chr8_2:12554813-12554832 None:intergenic 40.0%
ATTGGGATTGGGAATTGTTG+AGG - chr8_2:12554820-12554839 None:intergenic 40.0%
GTTAGTGTTAGGATTGGGAT+TGG - chr8_2:12554832-12554851 None:intergenic 40.0%
TTCCAGATCGAGATCGAATT+CGG - chr8_2:12554862-12554881 None:intergenic 40.0%
ATCCAAATCTCAACAACCGT+TGG + chr8_2:12554908-12554927 Msa1234830:CDS 40.0%
ATCCAACGGTTGTTGAGATT+TGG - chr8_2:12554913-12554932 None:intergenic 40.0%
!! AATCTGGCGTTGATATCCAA+CGG - chr8_2:12554927-12554946 None:intergenic 40.0%
!!! AGTTTTGAGAGCATCGAATC+TGG - chr8_2:12554943-12554962 None:intergenic 40.0%
TCTTCATCATCGGATACAGA+CGG - chr8_2:12555113-12555132 None:intergenic 40.0%
AGGATCGTGATCTTCATCAT+CGG - chr8_2:12555123-12555142 None:intergenic 40.0%
!!! ATTTTTTGACTGGTTTGCGG+CGG - chr8_2:12555184-12555203 None:intergenic 40.0%
ACATGTCGTTAGGTATGTGT+CGG + chr8_2:12555411-12555430 Msa1234830:intron 40.0%
AATCACCTTCAATAGGCCAA+AGG + chr8_2:12555845-12555864 Msa1234830:intron 40.0%
ATCACCTTCAATAGGCCAAA+GGG + chr8_2:12555846-12555865 Msa1234830:intron 40.0%
ATTACCCTTTGGCCTATTGA+AGG - chr8_2:12555853-12555872 None:intergenic 40.0%
!! CTTTGATGGCTTGCTTGATA+AGG + chr8_2:12555934-12555953 Msa1234830:intron 40.0%
! CTTTGACTATGGATTGCAGA+AGG + chr8_2:12555963-12555982 Msa1234830:intron 40.0%
AAATGTGAGACAACATCAGC+TGG - chr8_2:12556042-12556061 None:intergenic 40.0%
AGCTGATGTTGTCTCACATT+TGG + chr8_2:12556041-12556060 Msa1234830:intron 40.0%
TGGTGTACAAATTCTTCGCT+AGG - chr8_2:12556123-12556142 None:intergenic 40.0%
CTGGCCATATTAACTCTCAA+TGG + chr8_2:12556353-12556372 Msa1234830:CDS 40.0%
GGCAACCTATCAAAGTTAAC+TGG + chr8_2:12556462-12556481 Msa1234830:CDS 40.0%
TTATGTGGGATCAGAAGACT+GGG + chr8_2:12556906-12556925 Msa1234830:CDS 40.0%
! AAAAATAGACCTGCTGGCTA+CGG - chr8_2:12556961-12556980 None:intergenic 40.0%
ACTCCCTTGAACTGAGAATA+TGG - chr8_2:12557193-12557212 None:intergenic 40.0%
!! TTCTTCAGCATATCGACTTC+TGG + chr8_2:12557305-12557324 Msa1234830:intron 40.0%
! CTGGTGATGCACTGTTTAAT+TGG + chr8_2:12557324-12557343 Msa1234830:intron 40.0%
TTACCTGAAAGGAACAGACA+GGG - chr8_2:12557394-12557413 None:intergenic 40.0%
TAGACAAATACGCTGCAACT+GGG + chr8_2:12557426-12557445 Msa1234830:CDS 40.0%
AAACTCGGACAGTAAAAGTG+TGG + chr8_2:12557483-12557502 Msa1234830:CDS 40.0%
! AGTGTGGTGGAACTAACAAA+TGG + chr8_2:12557499-12557518 Msa1234830:CDS 40.0%
ACTGTTTATGTGGGTAACCT+TGG + chr8_2:12557848-12557867 Msa1234830:CDS 40.0%
TTATGTGGGTAACCTTGGAT+CGG + chr8_2:12557853-12557872 Msa1234830:CDS 40.0%
ATCGTCATTTCCATGCTCTT+GGG + chr8_2:12558258-12558277 Msa1234830:CDS 40.0%
TCCAGCGTGATAAAGGATTT+GGG + chr8_2:12558306-12558325 Msa1234830:CDS 40.0%
GAGATATAGTACTCATGCTG+AGG + chr8_2:12558334-12558353 Msa1234830:CDS 40.0%
TTTGCCACAGAGATAAGACT+GGG - chr8_2:12558387-12558406 None:intergenic 40.0%
! TTTTGCCACAGAGATAAGAC+TGG - chr8_2:12558388-12558407 None:intergenic 40.0%
! TTTTCACCTTTTGATGTCGC+TGG + chr8_2:12558422-12558441 Msa1234830:intron 40.0%
GAATATTGTGTGAAGGAGTG+TGG + chr8_2:12558492-12558511 Msa1234830:intron 40.0%
TCGTGTATATTTCACAGCAC+AGG - chr8_2:12558553-12558572 None:intergenic 40.0%
CCAAAATATCTGTTGCGGAT+AGG - chr8_2:12558729-12558748 None:intergenic 40.0%
! CCTATCCGCAACAGATATTT+TGG + chr8_2:12558726-12558745 Msa1234830:CDS 40.0%
!!! CAGATATTTTGGCATACGAG+CGG + chr8_2:12558737-12558756 Msa1234830:CDS 40.0%
AGGTGAAGGACGAGATAGAT+GGG - chr8_2:12554793-12554812 None:intergenic 45.0%
AAGGTGAAGGACGAGATAGA+TGG - chr8_2:12554794-12554813 None:intergenic 45.0%
! TCGGGGTTAGTGTTAGGATT+GGG - chr8_2:12554837-12554856 None:intergenic 45.0%
! ATCGGGGTTAGTGTTAGGAT+TGG - chr8_2:12554838-12554857 None:intergenic 45.0%
ATCGAGATCGAATTCGGGAT+CGG - chr8_2:12554856-12554875 None:intergenic 45.0%
TCCAGATCGAGATCGAATTC+GGG - chr8_2:12554861-12554880 None:intergenic 45.0%
TCGTCAGATACATCGGAAGA+GGG - chr8_2:12555146-12555165 None:intergenic 45.0%
ATCGTCGTCGTCAGATACAT+CGG - chr8_2:12555153-12555172 None:intergenic 45.0%
ATTGCAGAAGGACCGAGAAT+TGG + chr8_2:12555975-12555994 Msa1234830:intron 45.0%
! GCATGCGAAAAGTGTAGCAT+CGG - chr8_2:12556653-12556672 None:intergenic 45.0%
!! ACTTTGTTGGTGACTTGGCA+TGG + chr8_2:12556841-12556860 Msa1234830:intron 45.0%
GTTATGTGGGATCAGAAGAC+TGG + chr8_2:12556905-12556924 Msa1234830:CDS 45.0%
GTTCAAGGGAGTTGCACATT+AGG + chr8_2:12557201-12557220 Msa1234830:intron 45.0%
CTTACCTGAAAGGAACAGAC+AGG - chr8_2:12557395-12557414 None:intergenic 45.0%
! GTCTGTTCCTTTCAGGTAAG+TGG + chr8_2:12557395-12557414 Msa1234830:intron 45.0%
! TTCCTTTCAGGTAAGTGGCT+TGG + chr8_2:12557400-12557419 Msa1234830:intron 45.0%
TTCCAAGCCACTTACCTGAA+AGG - chr8_2:12557405-12557424 None:intergenic 45.0%
GTAGACAAATACGCTGCAAC+TGG + chr8_2:12557425-12557444 Msa1234830:CDS 45.0%
!! CCAAAGTTTTAGTAAGCCCC+CGG - chr8_2:12557633-12557652 None:intergenic 45.0%
ATCTCTTTACCGTCCTCTGT+AGG - chr8_2:12557788-12557807 None:intergenic 45.0%
GTGTACTGAGGATTGTTCTC+AGG - chr8_2:12557827-12557846 None:intergenic 45.0%
AGTTTCTTACCTCCGATCCA+AGG - chr8_2:12557868-12557887 None:intergenic 45.0%
! ACGACCTTTGGTGATTCTAC+AGG + chr8_2:12558217-12558236 Msa1234830:intron 45.0%
GTTGCCTGTAGAATCACCAA+AGG - chr8_2:12558224-12558243 None:intergenic 45.0%
! CAAGAGCATGGAAATGACGA+TGG - chr8_2:12558259-12558278 None:intergenic 45.0%
CATCGTCATTTCCATGCTCT+TGG + chr8_2:12558257-12558276 Msa1234830:CDS 45.0%
TCGTCATTTCCATGCTCTTG+GGG + chr8_2:12558259-12558278 Msa1234830:CDS 45.0%
GTCCAGCGTGATAAAGGATT+TGG + chr8_2:12558305-12558324 Msa1234830:CDS 45.0%
ACCCAAATCCTTTATCACGC+TGG - chr8_2:12558310-12558329 None:intergenic 45.0%
! AATGCCCAGTCTTATCTCTG+TGG + chr8_2:12558380-12558399 Msa1234830:CDS 45.0%
! AGAGGATTTGAAGCAGTTCC+AGG - chr8_2:12558676-12558695 None:intergenic 45.0%
CAGCTAGCAATGAGCAAGAT+GGG + chr8_2:12558760-12558779 Msa1234830:CDS 45.0%
TTAAGAGGATGCTGTGCTTG+AGG - chr8_2:12558811-12558830 None:intergenic 45.0%
! CCAATTGCAGCTTGCTTAAG+AGG - chr8_2:12558826-12558845 None:intergenic 45.0%
CCTCTTAAGCAAGCTGCAAT+TGG + chr8_2:12558823-12558842 Msa1234830:CDS 45.0%
TCGAGATCGAATTCGGGATC+GGG - chr8_2:12554855-12554874 None:intergenic 50.0%
TCCCGAATTCGATCTCGATC+TGG + chr8_2:12554857-12554876 Msa1234830:CDS 50.0%
CGATGTAGAGACAGTGATGC+CGG - chr8_2:12554979-12554998 None:intergenic 50.0%
CGGCATCACTGTCTCTACAT+CGG + chr8_2:12554977-12554996 Msa1234830:CDS 50.0%
TCAGATACATCGGAAGAGGG+AGG - chr8_2:12555143-12555162 None:intergenic 50.0%
GTCGTCAGATACATCGGAAG+AGG - chr8_2:12555147-12555166 None:intergenic 50.0%
! GTCGTTAGGTATGTGTCGGT+CGG + chr8_2:12555415-12555434 Msa1234830:intron 50.0%
! TTGTGTCGGCGAATCAGTGT+CGG + chr8_2:12555496-12555515 Msa1234830:intron 50.0%
GTTAACTGGGCATATGCCAG+TGG + chr8_2:12556476-12556495 Msa1234830:CDS 50.0%
ATATGCCAGTGGTCAGAGAG+AGG + chr8_2:12556487-12556506 Msa1234830:CDS 50.0%
TGTATCCTCTCTCTGACCAC+TGG - chr8_2:12556495-12556514 None:intergenic 50.0%
GGTCAGAGAGAGGATACATC+AGG + chr8_2:12556497-12556516 Msa1234830:CDS 50.0%
!! AGTGTAGCATCGGTAACCTC+AGG - chr8_2:12556643-12556662 None:intergenic 50.0%
ATCAGAAGACTGGGCGATCA+AGG + chr8_2:12556915-12556934 Msa1234830:CDS 50.0%
TCAGAAGACTGGGCGATCAA+GGG + chr8_2:12556916-12556935 Msa1234830:CDS 50.0%
TGTTTCATTCCGTAGCCAGC+AGG + chr8_2:12556949-12556968 Msa1234830:CDS 50.0%
ATGCCCTGTCTGTTCCTTTC+AGG + chr8_2:12557388-12557407 Msa1234830:intron 50.0%
! AACTGGGCAACAAAAGGTGC+TGG + chr8_2:12557442-12557461 Msa1234830:CDS 50.0%
! CTCGGACAGTAAAAGTGTGG+TGG + chr8_2:12557486-12557505 Msa1234830:CDS 50.0%
CCGGGGGCTTACTAAAACTT+TGG + chr8_2:12557630-12557649 Msa1234830:intron 50.0%
GTTCGTGTCCAGCGTGATAA+AGG + chr8_2:12558299-12558318 Msa1234830:CDS 50.0%
! GCAGCTCTGGCAATTCAGAT+GGG + chr8_2:12558356-12558375 Msa1234830:CDS 50.0%
AGTGAGCCAGCGACATCAAA+AGG - chr8_2:12558431-12558450 None:intergenic 50.0%
! GGATTTGAAGCAGTTCCAGG+TGG - chr8_2:12558673-12558692 None:intergenic 50.0%
ATATCTGTTGCGGATAGGCC+TGG - chr8_2:12558724-12558743 None:intergenic 50.0%
GCAGCTAGCAATGAGCAAGA+TGG + chr8_2:12558759-12558778 Msa1234830:CDS 50.0%
CTAGCAATGAGCAAGATGGG+TGG + chr8_2:12558763-12558782 Msa1234830:CDS 50.0%
! GCTGTGCAGCAGCAACATTT+TGG - chr8_2:12558879-12558898 None:intergenic 50.0%
! TCGGGATCGGGGTTAGTGTT+AGG - chr8_2:12554843-12554862 None:intergenic 55.0%
CGAGATCGAATTCGGGATCG+GGG - chr8_2:12554854-12554873 None:intergenic 55.0%
TCATCGGATACAGACGGCGA+CGG - chr8_2:12555107-12555126 None:intergenic 55.0%
!! TGTTGGTGACCTCAGTCCTG+AGG + chr8_2:12556624-12556643 Msa1234830:CDS 55.0%
CATCGGTAACCTCAGGACTG+AGG - chr8_2:12556636-12556655 None:intergenic 55.0%
CAGAAGACTGGGCGATCAAG+GGG + chr8_2:12556917-12556936 Msa1234830:CDS 55.0%
ACTGGGCGATCAAGGGGATT+TGG + chr8_2:12556923-12556942 Msa1234830:CDS 55.0%
CGCTGCAACTGGGCAACAAA+AGG + chr8_2:12557436-12557455 Msa1234830:CDS 55.0%
!! TGGGCAACAAAAGGTGCTGG+CGG + chr8_2:12557445-12557464 Msa1234830:CDS 55.0%
TGTGGGTAACCTTGGATCGG+AGG + chr8_2:12557856-12557875 Msa1234830:CDS 55.0%
CATTTCCATGCTCTTGGGGC+TGG + chr8_2:12558263-12558282 Msa1234830:CDS 55.0%
! TCTTGGGGCTGGTGTGATAG+AGG + chr8_2:12558274-12558293 Msa1234830:CDS 55.0%
TACTCATGCTGAGGCAGCTC+TGG + chr8_2:12558343-12558362 Msa1234830:CDS 55.0%
! GGCAGCTCTGGCAATTCAGA+TGG + chr8_2:12558355-12558374 Msa1234830:CDS 55.0%
GAAGCAGTTCCAGGTGGAGT+GGG - chr8_2:12558667-12558686 None:intergenic 55.0%
TGAAGCAGTTCCAGGTGGAG+TGG - chr8_2:12558668-12558687 None:intergenic 55.0%
AGCTGCAATTGGAGCAAGCC+AGG + chr8_2:12558834-12558853 Msa1234830:CDS 55.0%
GCAAGCCAGGCGATATACGA+CGG + chr8_2:12558847-12558866 Msa1234830:CDS 55.0%
CAAGCCAGGCGATATACGAC+GGG + chr8_2:12558848-12558867 Msa1234830:CDS 55.0%
AAGCCAGGCGATATACGACG+GGG + chr8_2:12558849-12558868 Msa1234830:CDS 55.0%
GACAGTGATGCCGGCAGGAT+TGG - chr8_2:12554970-12554989 None:intergenic 60.0%
GTAGAGACAGTGATGCCGGC+AGG - chr8_2:12554975-12554994 None:intergenic 60.0%
CGATCGCAGATCTGCTGCTC+TGG + chr8_2:12556334-12556353 Msa1234830:CDS 60.0%
TCACACCAGCCCCAAGAGCA+TGG - chr8_2:12558271-12558290 None:intergenic 60.0%
GTTGCGGATAGGCCTGGTAG+AGG - chr8_2:12558718-12558737 None:intergenic 60.0%
AGCCAGGCGATATACGACGG+GGG + chr8_2:12558850-12558869 Msa1234830:CDS 60.0%
ACAGACGGCGACGGAGGAGA+AGG - chr8_2:12555098-12555117 None:intergenic 65.0%
TCGGATACAGACGGCGACGG+AGG - chr8_2:12555104-12555123 None:intergenic 65.0%
! TAGAGGCGCAGCAGCAGGTG+GGG - chr8_2:12558701-12558720 None:intergenic 65.0%
! GTAGAGGCGCAGCAGCAGGT+GGG - chr8_2:12558702-12558721 None:intergenic 65.0%
GCCAGGCGATATACGACGGG+GGG + chr8_2:12558851-12558870 Msa1234830:CDS 65.0%
ACCCCCCGTCGTATATCGCC+TGG - chr8_2:12558855-12558874 None:intergenic 65.0%
GGGAGCAAGCCCACTCCACC+TGG + chr8_2:12558655-12558674 Msa1234830:CDS 70.0%
GCAGCAGCAGGTGGGGGAAG+AGG - chr8_2:12558694-12558713 None:intergenic 70.0%
! AGAGGCGCAGCAGCAGGTGG+GGG - chr8_2:12558700-12558719 None:intergenic 70.0%
! GGTAGAGGCGCAGCAGCAGG+TGG - chr8_2:12558703-12558722 None:intergenic 70.0%
! CCTGGTAGAGGCGCAGCAGC+AGG - chr8_2:12558706-12558725 None:intergenic 70.0%
CCTGCTGCTGCGCCTCTACC+AGG + chr8_2:12558703-12558722 Msa1234830:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 12554687 12559246 12554687 ID=Msa1234830;Name=Msa1234830
chr8_2 mRNA 12554687 12559246 12554687 ID=Msa1234830-mRNA-1;Parent=Msa1234830;Name=Msa1234830-mRNA-1;_AED=0.07;_eAED=0.08;_QI=0|0.77|0.7|1|1|1|10|328|513
chr8_2 exon 12554687 12555193 12554687 ID=Msa1234830-mRNA-1:exon:4819;Parent=Msa1234830-mRNA-1
chr8_2 exon 12556308 12556384 12556308 ID=Msa1234830-mRNA-1:exon:4820;Parent=Msa1234830-mRNA-1
chr8_2 exon 12556457 12556518 12556457 ID=Msa1234830-mRNA-1:exon:4821;Parent=Msa1234830-mRNA-1
chr8_2 exon 12556608 12556695 12556608 ID=Msa1234830-mRNA-1:exon:4822;Parent=Msa1234830-mRNA-1
chr8_2 exon 12556895 12556970 12556895 ID=Msa1234830-mRNA-1:exon:4823;Parent=Msa1234830-mRNA-1
chr8_2 exon 12557058 12557088 12557058 ID=Msa1234830-mRNA-1:exon:4824;Parent=Msa1234830-mRNA-1
chr8_2 exon 12557410 12557529 12557410 ID=Msa1234830-mRNA-1:exon:4825;Parent=Msa1234830-mRNA-1
chr8_2 exon 12557747 12557877 12557747 ID=Msa1234830-mRNA-1:exon:4826;Parent=Msa1234830-mRNA-1
chr8_2 exon 12558239 12558415 12558239 ID=Msa1234830-mRNA-1:exon:4827;Parent=Msa1234830-mRNA-1
chr8_2 exon 12558646 12559246 12558646 ID=Msa1234830-mRNA-1:exon:4828;Parent=Msa1234830-mRNA-1
chr8_2 CDS 12554687 12555193 12554687 ID=Msa1234830-mRNA-1:cds;Parent=Msa1234830-mRNA-1
chr8_2 CDS 12556308 12556384 12556308 ID=Msa1234830-mRNA-1:cds;Parent=Msa1234830-mRNA-1
chr8_2 CDS 12556457 12556518 12556457 ID=Msa1234830-mRNA-1:cds;Parent=Msa1234830-mRNA-1
chr8_2 CDS 12556608 12556695 12556608 ID=Msa1234830-mRNA-1:cds;Parent=Msa1234830-mRNA-1
chr8_2 CDS 12556895 12556970 12556895 ID=Msa1234830-mRNA-1:cds;Parent=Msa1234830-mRNA-1
chr8_2 CDS 12557058 12557088 12557058 ID=Msa1234830-mRNA-1:cds;Parent=Msa1234830-mRNA-1
chr8_2 CDS 12557410 12557529 12557410 ID=Msa1234830-mRNA-1:cds;Parent=Msa1234830-mRNA-1
chr8_2 CDS 12557747 12557877 12557747 ID=Msa1234830-mRNA-1:cds;Parent=Msa1234830-mRNA-1
chr8_2 CDS 12558239 12558415 12558239 ID=Msa1234830-mRNA-1:cds;Parent=Msa1234830-mRNA-1
chr8_2 CDS 12558646 12558918 12558646 ID=Msa1234830-mRNA-1:cds;Parent=Msa1234830-mRNA-1
chr8_2 three_prime_UTR 12558919 12559246 12558919 ID=Msa1234830-mRNA-1:three_prime_utr;Parent=Msa1234830-mRNA-1
Gene Sequence

>Msa1234830

ATGAGTCGAGGAAACAGAAAGCAGAAGAAACAACCAGAAGATGAAGTTGAACAAATGCTACGCGCCGCACAAGACGAAATCCTCTTAAACCTCTCTCTCAATTCCCATCTATCTCGTCCTTCACCTTCTACCTCAACAATTCCCAATCCCAATCCTAACACTAACCCCGATCCCGAATTCGATCTCGATCTGGAACGTCGATTTCAAGCTCTCAAATCAAAATCCAAATCTCAACAACCGTTGGATATCAACGCCAGATTCGATGCTCTCAAAACTAAATCCAATCCTGCCGGCATCACTGTCTCTACATCGGAAACTCAATTTCGGTATGAAAGTGAAGAGGAAGAAGATGAAGATGTTCAGATTCGGAAATTGATTGAATGGGCTAAAGATTCTGCGCGTCTTGATCCTTCTCCTCCGTCGCCGTCTGTATCCGATGATGAAGATCACGATCCTCCCTCTTCCGATGTATCTGACGACGACGATGATCAACACCGCCGCAAACCATCATCTTATGGATTCATTCATTACTTCGATCGCAGATCTGCTGCTCTGGCCATATTAACTCTCAATGGAAGGCATCTATTCGGGCAACCTATCAAAGTTAACTGGGCATATGCCAGTGGTCAGAGAGAGGATACATCAGGCCATTATAACATATTTGTTGGTGACCTCAGTCCTGAGGTTACCGATGCTACACTTTTCGCATGCTTTTCTGTCTACCAAAGTTGTTCAGATGCAAGAGTTATGTGGGATCAGAAGACTGGGCGATCAAGGGGATTTGGATTTGTTTCATTCCGTAGCCAGCAGGATGCTCAAAGTGCTATAAATGATTTGACCGGTAAGTGGCTTGGAAGTAGACAAATACGCTGCAACTGGGCAACAAAAGGTGCTGGCGGTATTGAAGAGAAGCAAAACTCGGACAGTAAAAGTGTGGTGGAACTAACAAATGGTTCATCTGATGGAATGCTAGATATTCAATTAACAAACATTTTTTATCCTACAGAGGACGGTAAAGAGATATCTAGCAATGATGTTCCTGAGAACAATCCTCAGTACACAACTGTTTATGTGGGTAACCTTGGATCGGAGGCAACACAGCTTGATCTCCATCGTCATTTCCATGCTCTTGGGGCTGGTGTGATAGAGGAAGTTCGTGTCCAGCGTGATAAAGGATTTGGGTTTGTGAGATATAGTACTCATGCTGAGGCAGCTCTGGCAATTCAGATGGGAAATGCCCAGTCTTATCTCTGTGGCAAAATAATTAAGTGCTCTTGGGGGAGCAAGCCCACTCCACCTGGAACTGCTTCAAATCCTCTTCCCCCACCTGCTGCTGCGCCTCTACCAGGCCTATCCGCAACAGATATTTTGGCATACGAGCGGCAGCTAGCAATGAGCAAGATGGGTGGAGTTCATGCTGCGTTGATGCATCCTCAAGCACAGCATCCTCTTAAGCAAGCTGCAATTGGAGCAAGCCAGGCGATATACGACGGGGGGTTCCAAAATGTTGCTGCTGCACAGCAAATGATGTATTATCAGTAA
Protein sequence

>Msa1234830

MSRGNRKQKKQPEDEVEQMLRAAQDEILLNLSLNSHLSRPSPSTSTIPNPNPNTNPDPEFDLDLERRFQALKSKSKSQQPLDINARFDALKTKSNPAGITVSTSETQFRYESEEEEDEDVQIRKLIEWAKDSARLDPSPPSPSVSDDEDHDPPSSDVSDDDDDQHRRKPSSYGFIHYFDRRSAALAILTLNGRHLFGQPIKVNWAYASGQREDTSGHYNIFVGDLSPEVTDATLFACFSVYQSCSDARVMWDQKTGRSRGFGFVSFRSQQDAQSAINDLTGKWLGSRQIRCNWATKGAGGIEEKQNSDSKSVVELTNGSSDGMLDIQLTNIFYPTEDGKEISSNDVPENNPQYTTVYVGNLGSEATQLDLHRHFHALGAGVIEEVRVQRDKGFGFVRYSTHAEAALAIQMGNAQSYLCGKIIKCSWGSKPTPPGTASNPLPPPAAAPLPGLSATDILAYERQLAMSKMGGVHAALMHPQAQHPLKQAAIGASQAIYDGGFQNVAAAQQMMYYQ*