AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1282190

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1282190 MtrunA17_Chr8g0353131 92.188 192 15 0 1 192 1 192 7.23e-133 370
Msa1282190 MtrunA17_Chr3g0084221 76.316 190 45 0 3 192 2 191 1.07e-107 306
Msa1282190 MtrunA17_Chr3g0084161 72.105 190 53 0 3 192 2 191 3.04e-105 300
Msa1282190 MtrunA17_Chr3g0084201 73.158 190 50 1 3 192 2 190 7.67e-102 291
Msa1282190 MtrunA17_Chr3g0084191 63.351 191 67 1 3 190 2 192 2.35e-85 260
Msa1282190 MtrunA17_Chr3g0082411 60.317 63 20 1 51 108 1 63 1.31e-19 79.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1282190 AT3G27890.1 75.401 187 46 0 5 191 10 196 2.11e-101 291

Find 50 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAATATCAATATGATTT+TGG 0.116343 8_3:+22418536 Msa1282190:three_prime_UTR
TAGTGCATTAGAGTAATTTA+AGG 0.190097 8_3:-22418506 None:intergenic
ATTAAAGTGGCAGCTCTTTC+TGG 0.193210 8_3:+22416926 Msa1282190:CDS
ATATATCAAGCAGATGATTT+AGG 0.273257 8_3:-22418703 None:intergenic
TTTGACCGATGTGAATAATT+TGG 0.280292 8_3:-22418680 None:intergenic
CATCTAGACCACCAAATGTT+TGG 0.329711 8_3:+22418227 Msa1282190:CDS
CAGAAACGTATATAATTAGT+AGG 0.358345 8_3:-22416807 None:intergenic
CATTGAAGAATGCAATTGAT+TGG 0.367565 8_3:+22418203 Msa1282190:CDS
ATCTAGACCACCAAATGTTT+GGG 0.385196 8_3:+22418228 Msa1282190:CDS
GATGACGGCGATTTGATTGA+TGG 0.402320 8_3:+22418406 Msa1282190:CDS
GGGTCGTCCTCGACAACATT+TGG 0.402898 8_3:-22416786 None:intergenic
CAATCAATTGCATTCTTCAA+TGG 0.420729 8_3:-22418202 None:intergenic
GCATTTACTCTGAGACTTCA+AGG 0.432779 8_3:+22418472 Msa1282190:CDS
TGGCGAAAAGCTTCAACAAC+AGG 0.452851 8_3:-22417189 None:intergenic
ATTGAAGAATGCAATTGATT+GGG 0.453647 8_3:+22418204 Msa1282190:CDS
TTTCCAGCCCAAACATTTGG+TGG 0.464756 8_3:-22418235 None:intergenic
ATAAGCACTGGAGGAGACTT+TGG 0.465806 8_3:+22418271 Msa1282190:CDS
AGAGAGAACCAGCACGGATA+AGG 0.467956 8_3:-22416979 None:intergenic
CACTCAGGCCTTATCCGTGC+TGG 0.477633 8_3:+22416971 Msa1282190:CDS
TTTGATTGATGGAGAGGCTA+AGG 0.510295 8_3:+22418417 Msa1282190:CDS
GCAGCAATTGAATTGAGTAA+AGG 0.518179 8_3:+22417078 Msa1282190:intron
AGACCACCAAATGTTTGGGC+TGG 0.522737 8_3:+22418232 Msa1282190:CDS
GGCTAAGGATAAGCTTAAGC+AGG 0.526766 8_3:+22418432 Msa1282190:CDS
TTGAGTAAAGGAGCAATTGA+AGG 0.530953 8_3:+22417090 Msa1282190:CDS
GCAGCTGCAATCATAAGCAC+TGG 0.535634 8_3:+22418259 Msa1282190:CDS
CGGCGATTTGATTGATGGAG+AGG 0.552841 8_3:+22418411 Msa1282190:CDS
CAATATCATCTACGCCAAGT+TGG 0.566210 8_3:+22418307 Msa1282190:CDS
TCAAGATCAGTGTTATACAT+TGG 0.571274 8_3:-22417147 None:intergenic
TAACACTGATCTTGAAAACA+AGG 0.588794 8_3:+22417155 Msa1282190:CDS
TATTATTCAAAATGTATGTG+AGG 0.591253 8_3:-22418635 None:intergenic
GAGTATAATTACTCTCTCAC+AGG 0.592007 8_3:+22417252 Msa1282190:CDS
CCTGCAAAATTCAATGATGA+CGG 0.593971 8_3:+22418391 Msa1282190:CDS
AGAAAAGCTTCGTACCACTC+AGG 0.601969 8_3:+22416956 Msa1282190:CDS
CGAAAAGCTTCAACAACAGG+TGG 0.602230 8_3:-22417186 None:intergenic
AGAAACGTATATAATTAGTA+GGG 0.604683 8_3:-22416806 None:intergenic
GTGAGAGAGTAATTATACTC+AGG 0.609716 8_3:-22417249 None:intergenic
ACTGGAGGAGACTTTGGCGG+TGG 0.610622 8_3:+22418277 Msa1282190:CDS
AGCACTGGAGGAGACTTTGG+CGG 0.611760 8_3:+22418274 Msa1282190:CDS
GGCAGCAGCAGTGATTAAAG+TGG 0.626013 8_3:+22416913 Msa1282190:CDS
ACAGAACACTAGCTAAGTCA+TGG 0.629230 8_3:+22416892 Msa1282190:exon
GATCGATATAGACTCCAACT+TGG 0.643158 8_3:-22418321 None:intergenic
AATGCTCTTAGAAAGAACTC+AGG 0.646361 8_3:-22418361 None:intergenic
GCTGCAATCATAAGCACTGG+AGG 0.650272 8_3:+22418262 Msa1282190:CDS
TATCAGCTTGAAGAATCTTG+TGG 0.652286 8_3:-22417209 None:intergenic
TTAGACCAAATTATTCACAT+CGG 0.654413 8_3:+22418675 Msa1282190:three_prime_UTR
AGACAAAGAGAGAACCAGCA+CGG 0.666728 8_3:-22416985 None:intergenic
GGGACTTCCAAATGTTGTCG+AGG 0.669720 8_3:+22416779 None:intergenic
AACACTGATCTTGAAAACAA+GGG 0.673165 8_3:+22417156 Msa1282190:CDS
AATGTCATTCAGAATGTGCA+TGG 0.673839 8_3:+22418573 Msa1282190:three_prime_UTR
CAGCACGGATAAGGCCTGAG+TGG 0.702973 8_3:-22416970 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCTTTTATTATTCTATTAT+TGG - chr8_3:22417344-22417363 None:intergenic 10.0%
!!! ATACTTAACAATTTTTATAA+TGG + chr8_3:22417640-22417659 Msa1282190:intron 10.0%
!!! TAATTCTTTTTATTACAAAT+TGG - chr8_3:22418116-22418135 None:intergenic 10.0%
!!! TTCTTTTATTATTCTATTAT+TGG - chr8_3:22417344-22417363 None:intergenic 10.0%
!!! ATACTTAACAATTTTTATAA+TGG + chr8_3:22417640-22417659 Msa1282190:intron 10.0%
!!! TAATTCTTTTTATTACAAAT+TGG - chr8_3:22418116-22418135 None:intergenic 10.0%
!!! TCTAGATTTTTTTTTTCTTT+TGG + chr8_3:22417306-22417325 Msa1282190:intron 15.0%
!! TAATTCATCAAAAGAATATA+CGG - chr8_3:22417461-22417480 None:intergenic 15.0%
!!! TATATTCTTTTGATGAATTA+AGG + chr8_3:22417461-22417480 Msa1282190:intron 15.0%
!! TTTATACCATTTAAATACTA+CGG - chr8_3:22417929-22417948 None:intergenic 15.0%
!!! TATATTTTTTCTTTTGAATC+AGG + chr8_3:22418039-22418058 Msa1282190:intron 15.0%
!!! TTCAAATATCAATATGATTT+TGG + chr8_3:22418536-22418555 Msa1282190:three_prime_UTR 15.0%
!!! TCTAGATTTTTTTTTTCTTT+TGG + chr8_3:22417306-22417325 Msa1282190:intron 15.0%
!! TAATTCATCAAAAGAATATA+CGG - chr8_3:22417461-22417480 None:intergenic 15.0%
!!! TATATTCTTTTGATGAATTA+AGG + chr8_3:22417461-22417480 Msa1282190:intron 15.0%
!! TTTATACCATTTAAATACTA+CGG - chr8_3:22417929-22417948 None:intergenic 15.0%
!!! TATATTTTTTCTTTTGAATC+AGG + chr8_3:22418039-22418058 Msa1282190:intron 15.0%
!!! TTCAAATATCAATATGATTT+TGG + chr8_3:22418536-22418555 Msa1282190:three_prime_UTR 15.0%
!! AGAAACGTATATAATTAGTA+GGG - chr8_3:22416809-22416828 None:intergenic 20.0%
!! AATTCATCAAAAGAATATAC+GGG - chr8_3:22417460-22417479 None:intergenic 20.0%
!!! CTTTGAAATTGTAATAGTTT+GGG + chr8_3:22417683-22417702 Msa1282190:intron 20.0%
!!! TCTTTTTATTACAAATTGGT+CGG - chr8_3:22418112-22418131 None:intergenic 20.0%
!! TATTATTCAAAATGTATGTG+AGG - chr8_3:22418638-22418657 None:intergenic 20.0%
!!! TTGATATATACTGACTTTTT+TGG + chr8_3:22418717-22418736 Msa1282190:three_prime_UTR 20.0%
!!! TTTTTGGTTTCAAGATATAT+TGG + chr8_3:22418733-22418752 Msa1282190:three_prime_UTR 20.0%
!! AGAAACGTATATAATTAGTA+GGG - chr8_3:22416809-22416828 None:intergenic 20.0%
!! AATTCATCAAAAGAATATAC+GGG - chr8_3:22417460-22417479 None:intergenic 20.0%
!!! CTTTGAAATTGTAATAGTTT+GGG + chr8_3:22417683-22417702 Msa1282190:intron 20.0%
!!! TCTTTTTATTACAAATTGGT+CGG - chr8_3:22418112-22418131 None:intergenic 20.0%
!! TATTATTCAAAATGTATGTG+AGG - chr8_3:22418638-22418657 None:intergenic 20.0%
!!! TTGATATATACTGACTTTTT+TGG + chr8_3:22418717-22418736 Msa1282190:three_prime_UTR 20.0%
!!! TTTTTGGTTTCAAGATATAT+TGG + chr8_3:22418733-22418752 Msa1282190:three_prime_UTR 20.0%
! CAGAAACGTATATAATTAGT+AGG - chr8_3:22416810-22416829 None:intergenic 25.0%
! ACAACCAATATATAGTACTT+AGG + chr8_3:22417495-22417514 Msa1282190:intron 25.0%
! CCAAACTATTACAATTTCAA+AGG - chr8_3:22417685-22417704 None:intergenic 25.0%
!!! CCTTTGAAATTGTAATAGTT+TGG + chr8_3:22417682-22417701 Msa1282190:intron 25.0%
! ATTGAAGAATGCAATTGATT+GGG + chr8_3:22418204-22418223 Msa1282190:CDS 25.0%
! TAGTGCATTAGAGTAATTTA+AGG - chr8_3:22418509-22418528 None:intergenic 25.0%
!! ATCAATATGATTTTGGACTT+TGG + chr8_3:22418543-22418562 Msa1282190:three_prime_UTR 25.0%
!! GAGGAAATAATACATTTTCA+AGG - chr8_3:22418619-22418638 None:intergenic 25.0%
! TTAGACCAAATTATTCACAT+CGG + chr8_3:22418675-22418694 Msa1282190:three_prime_UTR 25.0%
! ATATATCAAGCAGATGATTT+AGG - chr8_3:22418706-22418725 None:intergenic 25.0%
! CAGAAACGTATATAATTAGT+AGG - chr8_3:22416810-22416829 None:intergenic 25.0%
! ACAACCAATATATAGTACTT+AGG + chr8_3:22417495-22417514 Msa1282190:intron 25.0%
! CCAAACTATTACAATTTCAA+AGG - chr8_3:22417685-22417704 None:intergenic 25.0%
!!! CCTTTGAAATTGTAATAGTT+TGG + chr8_3:22417682-22417701 Msa1282190:intron 25.0%
! ATTGAAGAATGCAATTGATT+GGG + chr8_3:22418204-22418223 Msa1282190:CDS 25.0%
! TAGTGCATTAGAGTAATTTA+AGG - chr8_3:22418509-22418528 None:intergenic 25.0%
!! ATCAATATGATTTTGGACTT+TGG + chr8_3:22418543-22418562 Msa1282190:three_prime_UTR 25.0%
!! GAGGAAATAATACATTTTCA+AGG - chr8_3:22418619-22418638 None:intergenic 25.0%
! TTAGACCAAATTATTCACAT+CGG + chr8_3:22418675-22418694 Msa1282190:three_prime_UTR 25.0%
! ATATATCAAGCAGATGATTT+AGG - chr8_3:22418706-22418725 None:intergenic 25.0%
GTTGTAGTATTCAATTTCCA+TGG + chr8_3:22417043-22417062 Msa1282190:intron 30.0%
TCAAGATCAGTGTTATACAT+TGG - chr8_3:22417150-22417169 None:intergenic 30.0%
TAACACTGATCTTGAAAACA+AGG + chr8_3:22417155-22417174 Msa1282190:CDS 30.0%
AACACTGATCTTGAAAACAA+GGG + chr8_3:22417156-22417175 Msa1282190:CDS 30.0%
TGGACCTAAGTACTATATAT+TGG - chr8_3:22417502-22417521 None:intergenic 30.0%
TGATAGTCTCAACATTTCAA+TGG + chr8_3:22417718-22417737 Msa1282190:intron 30.0%
!! TTGTAGCTTTGAAATGATCA+TGG - chr8_3:22417818-22417837 None:intergenic 30.0%
TCTGAACCGTAGTATTTAAA+TGG + chr8_3:22417920-22417939 Msa1282190:intron 30.0%
GAATGTTTGATGAGGAAATT+CGG - chr8_3:22417995-22418014 None:intergenic 30.0%
ACACAAAATAAAGGTGAAGA+TGG - chr8_3:22418176-22418195 None:intergenic 30.0%
TGGAGCTAAACACAAAATAA+AGG - chr8_3:22418185-22418204 None:intergenic 30.0%
CAATCAATTGCATTCTTCAA+TGG - chr8_3:22418205-22418224 None:intergenic 30.0%
CATTGAAGAATGCAATTGAT+TGG + chr8_3:22418203-22418222 Msa1282190:CDS 30.0%
TTTGACCGATGTGAATAATT+TGG - chr8_3:22418683-22418702 None:intergenic 30.0%
GTTGTAGTATTCAATTTCCA+TGG + chr8_3:22417043-22417062 Msa1282190:intron 30.0%
TCAAGATCAGTGTTATACAT+TGG - chr8_3:22417150-22417169 None:intergenic 30.0%
TAACACTGATCTTGAAAACA+AGG + chr8_3:22417155-22417174 Msa1282190:CDS 30.0%
AACACTGATCTTGAAAACAA+GGG + chr8_3:22417156-22417175 Msa1282190:CDS 30.0%
TGGACCTAAGTACTATATAT+TGG - chr8_3:22417502-22417521 None:intergenic 30.0%
TGATAGTCTCAACATTTCAA+TGG + chr8_3:22417718-22417737 Msa1282190:intron 30.0%
!! TTGTAGCTTTGAAATGATCA+TGG - chr8_3:22417818-22417837 None:intergenic 30.0%
TCTGAACCGTAGTATTTAAA+TGG + chr8_3:22417920-22417939 Msa1282190:intron 30.0%
GAATGTTTGATGAGGAAATT+CGG - chr8_3:22417995-22418014 None:intergenic 30.0%
ACACAAAATAAAGGTGAAGA+TGG - chr8_3:22418176-22418195 None:intergenic 30.0%
TGGAGCTAAACACAAAATAA+AGG - chr8_3:22418185-22418204 None:intergenic 30.0%
CAATCAATTGCATTCTTCAA+TGG - chr8_3:22418205-22418224 None:intergenic 30.0%
CATTGAAGAATGCAATTGAT+TGG + chr8_3:22418203-22418222 Msa1282190:CDS 30.0%
TTTGACCGATGTGAATAATT+TGG - chr8_3:22418683-22418702 None:intergenic 30.0%
!! TCTGATCTTTTCAGGAGATT+AGG - chr8_3:22416860-22416879 None:intergenic 35.0%
! TTCTTGTGTCTGATCTTTTC+AGG - chr8_3:22416868-22416887 None:intergenic 35.0%
GCAGCAATTGAATTGAGTAA+AGG + chr8_3:22417078-22417097 Msa1282190:intron 35.0%
TTGAGTAAAGGAGCAATTGA+AGG + chr8_3:22417090-22417109 Msa1282190:CDS 35.0%
TATCAGCTTGAAGAATCTTG+TGG - chr8_3:22417212-22417231 None:intergenic 35.0%
GTGAGAGAGTAATTATACTC+AGG - chr8_3:22417252-22417271 None:intergenic 35.0%
GAGTATAATTACTCTCTCAC+AGG + chr8_3:22417252-22417271 Msa1282190:CDS 35.0%
GCAAATGTAGTACGTATCAT+TGG - chr8_3:22417417-22417436 None:intergenic 35.0%
!! TAGCTTTGAAATGATCATGG+TGG - chr8_3:22417815-22417834 None:intergenic 35.0%
AAAGTGAAAGAACCGACAAA+CGG + chr8_3:22417844-22417863 Msa1282190:intron 35.0%
!!! ATATCCAACCGCTTTTTCAT+CGG - chr8_3:22417879-22417898 None:intergenic 35.0%
CTATATCTCCGAATGTAGAA+TGG - chr8_3:22417962-22417981 None:intergenic 35.0%
TTCGGAGATATAGACACAAA+TGG + chr8_3:22417969-22417988 Msa1282190:intron 35.0%
AGAATGCAGAATGTTTGATG+AGG - chr8_3:22418003-22418022 None:intergenic 35.0%
ATCTAGACCACCAAATGTTT+GGG + chr8_3:22418228-22418247 Msa1282190:CDS 35.0%
AATGCTCTTAGAAAGAACTC+AGG - chr8_3:22418364-22418383 None:intergenic 35.0%
! TCATCATTGAATTTTGCAGG+TGG - chr8_3:22418391-22418410 None:intergenic 35.0%
CCTGCAAAATTCAATGATGA+CGG + chr8_3:22418391-22418410 Msa1282190:CDS 35.0%
AATGTCATTCAGAATGTGCA+TGG + chr8_3:22418573-22418592 Msa1282190:three_prime_UTR 35.0%
!! TCTGATCTTTTCAGGAGATT+AGG - chr8_3:22416860-22416879 None:intergenic 35.0%
! TTCTTGTGTCTGATCTTTTC+AGG - chr8_3:22416868-22416887 None:intergenic 35.0%
GCAGCAATTGAATTGAGTAA+AGG + chr8_3:22417078-22417097 Msa1282190:intron 35.0%
TTGAGTAAAGGAGCAATTGA+AGG + chr8_3:22417090-22417109 Msa1282190:CDS 35.0%
TATCAGCTTGAAGAATCTTG+TGG - chr8_3:22417212-22417231 None:intergenic 35.0%
GTGAGAGAGTAATTATACTC+AGG - chr8_3:22417252-22417271 None:intergenic 35.0%
GAGTATAATTACTCTCTCAC+AGG + chr8_3:22417252-22417271 Msa1282190:CDS 35.0%
GCAAATGTAGTACGTATCAT+TGG - chr8_3:22417417-22417436 None:intergenic 35.0%
!! TAGCTTTGAAATGATCATGG+TGG - chr8_3:22417815-22417834 None:intergenic 35.0%
AAAGTGAAAGAACCGACAAA+CGG + chr8_3:22417844-22417863 Msa1282190:intron 35.0%
!!! ATATCCAACCGCTTTTTCAT+CGG - chr8_3:22417879-22417898 None:intergenic 35.0%
CTATATCTCCGAATGTAGAA+TGG - chr8_3:22417962-22417981 None:intergenic 35.0%
TTCGGAGATATAGACACAAA+TGG + chr8_3:22417969-22417988 Msa1282190:intron 35.0%
AGAATGCAGAATGTTTGATG+AGG - chr8_3:22418003-22418022 None:intergenic 35.0%
ATCTAGACCACCAAATGTTT+GGG + chr8_3:22418228-22418247 Msa1282190:CDS 35.0%
AATGCTCTTAGAAAGAACTC+AGG - chr8_3:22418364-22418383 None:intergenic 35.0%
! TCATCATTGAATTTTGCAGG+TGG - chr8_3:22418391-22418410 None:intergenic 35.0%
CCTGCAAAATTCAATGATGA+CGG + chr8_3:22418391-22418410 Msa1282190:CDS 35.0%
AATGTCATTCAGAATGTGCA+TGG + chr8_3:22418573-22418592 Msa1282190:three_prime_UTR 35.0%
ACAGAACACTAGCTAAGTCA+TGG + chr8_3:22416892-22416911 Msa1282190:exon 40.0%
ATTAAAGTGGCAGCTCTTTC+TGG + chr8_3:22416926-22416945 Msa1282190:CDS 40.0%
ACTGAAAAATCGCACGACAA+CGG + chr8_3:22417364-22417383 Msa1282190:intron 40.0%
GCAGGTGTATTGTTGTAATC+TGG - chr8_3:22417522-22417541 None:intergenic 40.0%
AATCAAGCGCTAAAGCTATC+GGG - chr8_3:22417751-22417770 None:intergenic 40.0%
CAATCAAGCGCTAAAGCTAT+CGG - chr8_3:22417752-22417771 None:intergenic 40.0%
! GATCATGGTGGAAAGTCATT+GGG - chr8_3:22417803-22417822 None:intergenic 40.0%
! TGATCATGGTGGAAAGTCAT+TGG - chr8_3:22417804-22417823 None:intergenic 40.0%
GGACAATTCCGATGAAAAAG+CGG + chr8_3:22417868-22417887 Msa1282190:intron 40.0%
AATTCCGATGAAAAAGCGGT+TGG + chr8_3:22417872-22417891 Msa1282190:intron 40.0%
GATGAAAAAGCGGTTGGATA+TGG + chr8_3:22417878-22417897 Msa1282190:intron 40.0%
AACTGCGACCATTCTACATT+CGG + chr8_3:22417951-22417970 Msa1282190:intron 40.0%
CATCTAGACCACCAAATGTT+TGG + chr8_3:22418227-22418246 Msa1282190:CDS 40.0%
CAATATCATCTACGCCAAGT+TGG + chr8_3:22418307-22418326 Msa1282190:CDS 40.0%
GATCGATATAGACTCCAACT+TGG - chr8_3:22418324-22418343 None:intergenic 40.0%
! CCGTCATCATTGAATTTTGC+AGG - chr8_3:22418394-22418413 None:intergenic 40.0%
TTTGATTGATGGAGAGGCTA+AGG + chr8_3:22418417-22418436 Msa1282190:CDS 40.0%
! GCAGGTTCTTTTATCTTTGC+AGG + chr8_3:22418450-22418469 Msa1282190:CDS 40.0%
!! GCATTTACTCTGAGACTTCA+AGG + chr8_3:22418472-22418491 Msa1282190:CDS 40.0%
ACAGAACACTAGCTAAGTCA+TGG + chr8_3:22416892-22416911 Msa1282190:exon 40.0%
ATTAAAGTGGCAGCTCTTTC+TGG + chr8_3:22416926-22416945 Msa1282190:CDS 40.0%
ACTGAAAAATCGCACGACAA+CGG + chr8_3:22417364-22417383 Msa1282190:intron 40.0%
GCAGGTGTATTGTTGTAATC+TGG - chr8_3:22417522-22417541 None:intergenic 40.0%
AATCAAGCGCTAAAGCTATC+GGG - chr8_3:22417751-22417770 None:intergenic 40.0%
CAATCAAGCGCTAAAGCTAT+CGG - chr8_3:22417752-22417771 None:intergenic 40.0%
! GATCATGGTGGAAAGTCATT+GGG - chr8_3:22417803-22417822 None:intergenic 40.0%
! TGATCATGGTGGAAAGTCAT+TGG - chr8_3:22417804-22417823 None:intergenic 40.0%
GGACAATTCCGATGAAAAAG+CGG + chr8_3:22417868-22417887 Msa1282190:intron 40.0%
AATTCCGATGAAAAAGCGGT+TGG + chr8_3:22417872-22417891 Msa1282190:intron 40.0%
GATGAAAAAGCGGTTGGATA+TGG + chr8_3:22417878-22417897 Msa1282190:intron 40.0%
AACTGCGACCATTCTACATT+CGG + chr8_3:22417951-22417970 Msa1282190:intron 40.0%
CATCTAGACCACCAAATGTT+TGG + chr8_3:22418227-22418246 Msa1282190:CDS 40.0%
CAATATCATCTACGCCAAGT+TGG + chr8_3:22418307-22418326 Msa1282190:CDS 40.0%
GATCGATATAGACTCCAACT+TGG - chr8_3:22418324-22418343 None:intergenic 40.0%
! CCGTCATCATTGAATTTTGC+AGG - chr8_3:22418394-22418413 None:intergenic 40.0%
TTTGATTGATGGAGAGGCTA+AGG + chr8_3:22418417-22418436 Msa1282190:CDS 40.0%
! GCAGGTTCTTTTATCTTTGC+AGG + chr8_3:22418450-22418469 Msa1282190:CDS 40.0%
!! GCATTTACTCTGAGACTTCA+AGG + chr8_3:22418472-22418491 Msa1282190:CDS 40.0%
! GTGGTACGAAGCTTTTCTGA+GGG - chr8_3:22416954-22416973 None:intergenic 45.0%
! AGTGGTACGAAGCTTTTCTG+AGG - chr8_3:22416955-22416974 None:intergenic 45.0%
AGAAAAGCTTCGTACCACTC+AGG + chr8_3:22416956-22416975 Msa1282190:CDS 45.0%
AGACAAAGAGAGAACCAGCA+CGG - chr8_3:22416988-22417007 None:intergenic 45.0%
GCTGCAATCATCATCAACCA+TGG - chr8_3:22417063-22417082 None:intergenic 45.0%
CGAAAAGCTTCAACAACAGG+TGG - chr8_3:22417189-22417208 None:intergenic 45.0%
TGGCGAAAAGCTTCAACAAC+AGG - chr8_3:22417192-22417211 None:intergenic 45.0%
CTGAAAAATCGCACGACAAC+GGG + chr8_3:22417365-22417384 Msa1282190:intron 45.0%
AAGGAGCTTTGCTAGCAAAG+GGG - chr8_3:22417575-22417594 None:intergenic 45.0%
GAAGGAGCTTTGCTAGCAAA+GGG - chr8_3:22417576-22417595 None:intergenic 45.0%
TGAAGGAGCTTTGCTAGCAA+AGG - chr8_3:22417577-22417596 None:intergenic 45.0%
CATACATAGACACCGAAGAG+TGG - chr8_3:22417616-22417635 None:intergenic 45.0%
!! CGATAGCTTTAGCGCTTGAT+TGG + chr8_3:22417750-22417769 Msa1282190:intron 45.0%
!! GCGCTTGATTGGTAATGTGT+AGG + chr8_3:22417761-22417780 Msa1282190:intron 45.0%
TTTCCAGCCCAAACATTTGG+TGG - chr8_3:22418238-22418257 None:intergenic 45.0%
! GCTTTTCCAGCCCAAACATT+TGG - chr8_3:22418241-22418260 None:intergenic 45.0%
!! ATAAGCACTGGAGGAGACTT+TGG + chr8_3:22418271-22418290 Msa1282190:CDS 45.0%
! GATGACGGCGATTTGATTGA+TGG + chr8_3:22418406-22418425 Msa1282190:CDS 45.0%
GGCTAAGGATAAGCTTAAGC+AGG + chr8_3:22418432-22418451 Msa1282190:CDS 45.0%
! GTGGTACGAAGCTTTTCTGA+GGG - chr8_3:22416954-22416973 None:intergenic 45.0%
! AGTGGTACGAAGCTTTTCTG+AGG - chr8_3:22416955-22416974 None:intergenic 45.0%
AGAAAAGCTTCGTACCACTC+AGG + chr8_3:22416956-22416975 Msa1282190:CDS 45.0%
AGACAAAGAGAGAACCAGCA+CGG - chr8_3:22416988-22417007 None:intergenic 45.0%
GCTGCAATCATCATCAACCA+TGG - chr8_3:22417063-22417082 None:intergenic 45.0%
CGAAAAGCTTCAACAACAGG+TGG - chr8_3:22417189-22417208 None:intergenic 45.0%
TGGCGAAAAGCTTCAACAAC+AGG - chr8_3:22417192-22417211 None:intergenic 45.0%
CTGAAAAATCGCACGACAAC+GGG + chr8_3:22417365-22417384 Msa1282190:intron 45.0%
AAGGAGCTTTGCTAGCAAAG+GGG - chr8_3:22417575-22417594 None:intergenic 45.0%
GAAGGAGCTTTGCTAGCAAA+GGG - chr8_3:22417576-22417595 None:intergenic 45.0%
TGAAGGAGCTTTGCTAGCAA+AGG - chr8_3:22417577-22417596 None:intergenic 45.0%
CATACATAGACACCGAAGAG+TGG - chr8_3:22417616-22417635 None:intergenic 45.0%
!! CGATAGCTTTAGCGCTTGAT+TGG + chr8_3:22417750-22417769 Msa1282190:intron 45.0%
!! GCGCTTGATTGGTAATGTGT+AGG + chr8_3:22417761-22417780 Msa1282190:intron 45.0%
TTTCCAGCCCAAACATTTGG+TGG - chr8_3:22418238-22418257 None:intergenic 45.0%
! GCTTTTCCAGCCCAAACATT+TGG - chr8_3:22418241-22418260 None:intergenic 45.0%
!! ATAAGCACTGGAGGAGACTT+TGG + chr8_3:22418271-22418290 Msa1282190:CDS 45.0%
! GATGACGGCGATTTGATTGA+TGG + chr8_3:22418406-22418425 Msa1282190:CDS 45.0%
GGCTAAGGATAAGCTTAAGC+AGG + chr8_3:22418432-22418451 Msa1282190:CDS 45.0%
GGCAGCAGCAGTGATTAAAG+TGG + chr8_3:22416913-22416932 Msa1282190:CDS 50.0%
AGAGAGAACCAGCACGGATA+AGG - chr8_3:22416982-22417001 None:intergenic 50.0%
TAGACACCGAAGAGTGGCAA+TGG - chr8_3:22417610-22417629 None:intergenic 50.0%
AGCGCTAAAGCTATCGGGTA+GGG - chr8_3:22417746-22417765 None:intergenic 50.0%
AAGCGCTAAAGCTATCGGGT+AGG - chr8_3:22417747-22417766 None:intergenic 50.0%
! CATGGTGGAAAGTCATTGGG+AGG - chr8_3:22417800-22417819 None:intergenic 50.0%
GTGAAAGAACCGACAAACGG+TGG + chr8_3:22417847-22417866 Msa1282190:intron 50.0%
CGGAATTGTCCACCGTTTGT+CGG - chr8_3:22417859-22417878 None:intergenic 50.0%
AAAGCGGTTGGATATGGCTC+AGG + chr8_3:22417884-22417903 Msa1282190:intron 50.0%
AAGCGGTTGGATATGGCTCA+GGG + chr8_3:22417885-22417904 Msa1282190:intron 50.0%
AGACCACCAAATGTTTGGGC+TGG + chr8_3:22418232-22418251 Msa1282190:CDS 50.0%
GCAGCTGCAATCATAAGCAC+TGG + chr8_3:22418259-22418278 Msa1282190:CDS 50.0%
!! GCTGCAATCATAAGCACTGG+AGG + chr8_3:22418262-22418281 Msa1282190:CDS 50.0%
CGGCGATTTGATTGATGGAG+AGG + chr8_3:22418411-22418430 Msa1282190:CDS 50.0%
GGCAGCAGCAGTGATTAAAG+TGG + chr8_3:22416913-22416932 Msa1282190:CDS 50.0%
AGAGAGAACCAGCACGGATA+AGG - chr8_3:22416982-22417001 None:intergenic 50.0%
TAGACACCGAAGAGTGGCAA+TGG - chr8_3:22417610-22417629 None:intergenic 50.0%
AGCGCTAAAGCTATCGGGTA+GGG - chr8_3:22417746-22417765 None:intergenic 50.0%
AAGCGCTAAAGCTATCGGGT+AGG - chr8_3:22417747-22417766 None:intergenic 50.0%
! CATGGTGGAAAGTCATTGGG+AGG - chr8_3:22417800-22417819 None:intergenic 50.0%
GTGAAAGAACCGACAAACGG+TGG + chr8_3:22417847-22417866 Msa1282190:intron 50.0%
CGGAATTGTCCACCGTTTGT+CGG - chr8_3:22417859-22417878 None:intergenic 50.0%
AAAGCGGTTGGATATGGCTC+AGG + chr8_3:22417884-22417903 Msa1282190:intron 50.0%
AAGCGGTTGGATATGGCTCA+GGG + chr8_3:22417885-22417904 Msa1282190:intron 50.0%
AGACCACCAAATGTTTGGGC+TGG + chr8_3:22418232-22418251 Msa1282190:CDS 50.0%
GCAGCTGCAATCATAAGCAC+TGG + chr8_3:22418259-22418278 Msa1282190:CDS 50.0%
!! GCTGCAATCATAAGCACTGG+AGG + chr8_3:22418262-22418281 Msa1282190:CDS 50.0%
CGGCGATTTGATTGATGGAG+AGG + chr8_3:22418411-22418430 Msa1282190:CDS 50.0%
GACGGGGTTATATTCCCTCG+AGG - chr8_3:22417551-22417570 None:intergenic 55.0%
TTTGCTAGCAAAGGGGCGAC+GGG - chr8_3:22417568-22417587 None:intergenic 55.0%
CTTTGCTAGCAAAGGGGCGA+CGG - chr8_3:22417569-22417588 None:intergenic 55.0%
CGAAGAGTGGCAATGGCAAG+GGG - chr8_3:22417603-22417622 None:intergenic 55.0%
CCGAAGAGTGGCAATGGCAA+GGG - chr8_3:22417604-22417623 None:intergenic 55.0%
CCCTTGCCATTGCCACTCTT+CGG + chr8_3:22417601-22417620 Msa1282190:intron 55.0%
ACCGAAGAGTGGCAATGGCA+AGG - chr8_3:22417605-22417624 None:intergenic 55.0%
! AGCACTGGAGGAGACTTTGG+CGG + chr8_3:22418274-22418293 Msa1282190:CDS 55.0%
GACGGGGTTATATTCCCTCG+AGG - chr8_3:22417551-22417570 None:intergenic 55.0%
TTTGCTAGCAAAGGGGCGAC+GGG - chr8_3:22417568-22417587 None:intergenic 55.0%
CTTTGCTAGCAAAGGGGCGA+CGG - chr8_3:22417569-22417588 None:intergenic 55.0%
CGAAGAGTGGCAATGGCAAG+GGG - chr8_3:22417603-22417622 None:intergenic 55.0%
CCGAAGAGTGGCAATGGCAA+GGG - chr8_3:22417604-22417623 None:intergenic 55.0%
CCCTTGCCATTGCCACTCTT+CGG + chr8_3:22417601-22417620 Msa1282190:intron 55.0%
ACCGAAGAGTGGCAATGGCA+AGG - chr8_3:22417605-22417624 None:intergenic 55.0%
! AGCACTGGAGGAGACTTTGG+CGG + chr8_3:22418274-22418293 Msa1282190:CDS 55.0%
CAGCACGGATAAGGCCTGAG+TGG - chr8_3:22416973-22416992 None:intergenic 60.0%
! CACTCAGGCCTTATCCGTGC+TGG + chr8_3:22416971-22416990 Msa1282190:CDS 60.0%
TACACCTGCATCGTGCCTCG+AGG + chr8_3:22417533-22417552 Msa1282190:intron 60.0%
ACACCTGCATCGTGCCTCGA+GGG + chr8_3:22417534-22417553 Msa1282190:intron 60.0%
ATTCCCTCGAGGCACGATGC+AGG - chr8_3:22417540-22417559 None:intergenic 60.0%
TTGCTAGCAAAGGGGCGACG+GGG - chr8_3:22417567-22417586 None:intergenic 60.0%
GCAATGGCAAGGGGCAGTGA+AGG - chr8_3:22417594-22417613 None:intergenic 60.0%
ACTGGAGGAGACTTTGGCGG+TGG + chr8_3:22418277-22418296 Msa1282190:CDS 60.0%
CAGCACGGATAAGGCCTGAG+TGG - chr8_3:22416973-22416992 None:intergenic 60.0%
! CACTCAGGCCTTATCCGTGC+TGG + chr8_3:22416971-22416990 Msa1282190:CDS 60.0%
TACACCTGCATCGTGCCTCG+AGG + chr8_3:22417533-22417552 Msa1282190:intron 60.0%
ACACCTGCATCGTGCCTCGA+GGG + chr8_3:22417534-22417553 Msa1282190:intron 60.0%
ATTCCCTCGAGGCACGATGC+AGG - chr8_3:22417540-22417559 None:intergenic 60.0%
TTGCTAGCAAAGGGGCGACG+GGG - chr8_3:22417567-22417586 None:intergenic 60.0%
GCAATGGCAAGGGGCAGTGA+AGG - chr8_3:22417594-22417613 None:intergenic 60.0%
ACTGGAGGAGACTTTGGCGG+TGG + chr8_3:22418277-22418296 Msa1282190:CDS 60.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 22416788 22418785 22416788 ID=Msa1282190;Name=Msa1282190
chr8_3 mRNA 22416788 22418785 22416788 ID=Msa1282190-mRNA-1;Parent=Msa1282190;Name=Msa1282190-mRNA-1;_AED=0.25;_eAED=0.25;_QI=123|1|1|1|1|1|3|281|192
chr8_3 exon 22416788 22416992 22416788 ID=Msa1282190-mRNA-1:exon:8016;Parent=Msa1282190-mRNA-1
chr8_3 exon 22417082 22417273 22417082 ID=Msa1282190-mRNA-1:exon:8017;Parent=Msa1282190-mRNA-1
chr8_3 exon 22418200 22418785 22418200 ID=Msa1282190-mRNA-1:exon:8018;Parent=Msa1282190-mRNA-1
chr8_3 five_prime_UTR 22416788 22416910 22416788 ID=Msa1282190-mRNA-1:five_prime_utr;Parent=Msa1282190-mRNA-1
chr8_3 CDS 22416911 22416992 22416911 ID=Msa1282190-mRNA-1:cds;Parent=Msa1282190-mRNA-1
chr8_3 CDS 22417082 22417273 22417082 ID=Msa1282190-mRNA-1:cds;Parent=Msa1282190-mRNA-1
chr8_3 CDS 22418200 22418504 22418200 ID=Msa1282190-mRNA-1:cds;Parent=Msa1282190-mRNA-1
chr8_3 three_prime_UTR 22418505 22418785 22418505 ID=Msa1282190-mRNA-1:three_prime_utr;Parent=Msa1282190-mRNA-1
Gene Sequence

>Msa1282190

ATGGCAGCAGCAGTGATTAAAGTGGCAGCTCTTTCTGGTTCCCTCAGAAAAGCTTCGTACCACTCAGGCCTTATCCGTGCTGCAATTGAATTGAGTAAAGGAGCAATTGAAGGCATTGAGATTGAGTTCATTGATATTTCAACTCTACCAATGTATAACACTGATCTTGAAAACAAGGGAACTTATCCACCTGTTGTTGAAGCTTTTCGCCACAAGATTCTTCAAGCTGATAGTGTTCTTTTTGCTTCTCCTGAGTATAATTACTCTCTCACAGCTCCATTGAAGAATGCAATTGATTGGGCATCTAGACCACCAAATGTTTGGGCTGGAAAAGCAGCTGCAATCATAAGCACTGGAGGAGACTTTGGCGGTGGAAGATCACAATATCATCTACGCCAAGTTGGAGTCTATATCGATCTTCATTTCATCAATAAACCTGAGTTCTTTCTAAGAGCATTTCAGCCACCTGCAAAATTCAATGATGACGGCGATTTGATTGATGGAGAGGCTAAGGATAAGCTTAAGCAGGTTCTTTTATCTTTGCAGGCATTTACTCTGAGACTTCAAGGCAAAAGCTGA
Protein sequence

>Msa1282190

MAAAVIKVAALSGSLRKASYHSGLIRAAIELSKGAIEGIEIEFIDISTLPMYNTDLENKGTYPPVVEAFRHKILQADSVLFASPEYNYSLTAPLKNAIDWASRPPNVWAGKAAAIISTGGDFGGGRSQYHLRQVGVYIDLHFINKPEFFLRAFQPPAKFNDDGDLIDGEAKDKLKQVLLSLQAFTLRLQGKS*