AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0826320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0826320 MtrunA17_Chr4g0014311 42.857 168 69 3 104 244 118 285 3.22e-32 119
Msa0826320 MtrunA17_Chr8g0365271 48.227 141 62 3 114 243 52 192 4.57e-31 113
Msa0826320 MtrunA17_Chr5g0444831 47.857 140 62 3 115 243 41 180 5.39e-30 110
Msa0826320 MtrunA17_Chr7g0270411 44.961 129 60 2 126 243 1 129 5.10e-25 95.9
Msa0826320 MtrunA17_Chr3g0138341 66.000 50 17 0 104 153 52 101 8.21e-18 77.4
Msa0826320 MtrunA17_Chr2g0300061 42.667 75 43 0 112 186 13 87 1.84e-17 75.1
Msa0826320 MtrunA17_Chr1g0212881 38.462 65 37 1 113 177 35 96 7.86e-13 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 107 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGCCAAAACCATCAAAATT+AGG 0.132100 5_3:+66667892 None:intergenic
TGGGAAACAAATGTTGTTTA+AGG 0.242210 5_3:-66665212 Msa0826320:CDS
TAAACAAAACAACATATAAA+TGG 0.265264 5_3:+66664508 None:intergenic
GAATATTGGTGCGAGTTTAT+TGG 0.269739 5_3:+66667756 None:intergenic
GAAAGGCTTGCTGCAACTTT+AGG 0.274755 5_3:-66667157 Msa0826320:CDS
TTCCCAACCACTTTGCTACT+TGG 0.290059 5_3:+66664758 None:intergenic
TGTGACTGTCCACAAAATTA+AGG 0.297574 5_3:-66665101 Msa0826320:intron
CATCGACATCGGACATATTT+CGG 0.315042 5_3:+66667445 None:intergenic
ATGGGAACTTAAAGCATAAT+TGG 0.316343 5_3:-66665177 Msa0826320:CDS
GACGGATGTGGCGGGGATAT+TGG 0.329689 5_3:+66667577 None:intergenic
TAATCTCCATTCATCTCATT+TGG 0.330111 5_3:-66667348 Msa0826320:intron
AGCCAAAACCATCAAAATTA+GGG 0.360232 5_3:+66667893 None:intergenic
ATCAATGCCAATACGGTTGC+CGG 0.366747 5_3:+66667512 None:intergenic
TCAGTTGCGGCTGATGAATC+TGG 0.378282 5_3:+66667476 None:intergenic
GGAGAATTAGCGGCGGCGTT+AGG 0.378302 5_3:+66667533 None:intergenic
TTGGGAACAGTTGGATTGTA+TGG 0.379258 5_3:+66667831 None:intergenic
TGTTTAATTGTGCAGGGAAC+CGG 0.393983 5_3:-66665271 Msa0826320:intron
TCGGAATGATCCAGAGAGTT+TGG 0.403460 5_3:+66667604 None:intergenic
TGGTGCGAGTTTATTGGATT+AGG 0.415397 5_3:+66667762 None:intergenic
TCTCCCACCTCTACCGTCTC+TGG 0.417810 5_3:-66667735 Msa0826320:five_prime_UTR
ATCTTCTTCACCAAACTCTC+TGG 0.418334 5_3:-66667614 Msa0826320:five_prime_UTR
TGAAAGACATTGGGAACAGT+TGG 0.423723 5_3:+66667822 None:intergenic
GTGTAGATCCGGCTGTTCTC+AGG 0.427894 5_3:+66667417 None:intergenic
GACTTAAAATTGAAAGACAT+TGG 0.431655 5_3:+66667812 None:intergenic
AACGCTAGGTTTGAGGAATT+AGG 0.434175 5_3:-66667377 Msa0826320:CDS
TTGCGAGTATCCAATTGATC+TGG 0.441282 5_3:-66665245 Msa0826320:CDS
GTTGTTTAAGGTGGAATTCA+CGG 0.447146 5_3:-66665200 Msa0826320:CDS
ATCCAACGGCCATAATAGTT+TGG 0.449119 5_3:+66667870 None:intergenic
CGGTAGAGGTGGGAGAATAT+TGG 0.449629 5_3:+66667742 None:intergenic
ACTTAAAATTGAAAGACATT+GGG 0.460714 5_3:+66667813 None:intergenic
ATACCGGAGGGAGGCAATGA+AGG 0.462165 5_3:+66667705 None:intergenic
GCTGATGAACGCTAGGTTTG+AGG 0.467581 5_3:-66667384 Msa0826320:CDS
CTCCAAACTATTATGGCCGT+TGG 0.468523 5_3:-66667872 Msa0826320:five_prime_UTR
CGAGGTTGCAAGTGTAGATC+CGG 0.469365 5_3:+66667406 None:intergenic
TAATTCTCCGGCAACCGTAT+TGG 0.469409 5_3:-66667519 Msa0826320:five_prime_UTR
GCGTTGATCCACATCGTCTA+AGG 0.475507 5_3:-66664588 Msa0826320:three_prime_UTR
TTTAATAATTGCAGTTCCGT+TGG 0.476199 5_3:-66666314 Msa0826320:intron
GAAACAAATGTTGTTTAAGG+TGG 0.479313 5_3:-66665209 Msa0826320:CDS
TTGACGCAATGAACTTTGAC+AGG 0.484199 5_3:+66664668 None:intergenic
CTCCCTTCATTGCCTCCCTC+CGG 0.488953 5_3:-66667708 Msa0826320:five_prime_UTR
GAGATTGTTGCAGAGAAGAA+AGG 0.502375 5_3:-66667174 Msa0826320:CDS
TTCGGTGGTTACATCAGTTG+CGG 0.506253 5_3:+66667463 None:intergenic
CATTAAAACAATGATACTAC+TGG 0.507765 5_3:+66664624 None:intergenic
GACATACCAAATGAGATGAA+TGG 0.509003 5_3:+66667342 None:intergenic
CACCAAGTAGCAAAGTGGTT+GGG 0.514111 5_3:-66664760 Msa0826320:CDS
AGTTCCAGAGACGGTAGAGG+TGG 0.514295 5_3:+66667731 None:intergenic
ACGACAGATGGAGGCAATGA+CGG 0.515183 5_3:+66667641 None:intergenic
TACGGTTGCCGGAGAATTAG+CGG 0.516224 5_3:+66667523 None:intergenic
ACACAGTTGACGGATGTGGC+GGG 0.519380 5_3:+66667569 None:intergenic
CGGCATGGAAGATGAGATAC+CGG 0.520453 5_3:+66667689 None:intergenic
CGACAGATGGAGGCAATGAC+GGG 0.520995 5_3:+66667642 None:intergenic
CCCTCTTGAACATGCTCCAA+CGG 0.524368 5_3:+66666298 None:intergenic
AGAGAAATTGACACAGTTGA+CGG 0.527126 5_3:+66667559 None:intergenic
TAACGCCGCCGCTAATTCTC+CGG 0.529028 5_3:-66667531 Msa0826320:five_prime_UTR
CAACCTCGCTGATGAACGCT+AGG 0.533506 5_3:-66667391 Msa0826320:CDS
ATTGCCATAACCAACATCTC+AGG 0.534276 5_3:+66664955 None:intergenic
ACTTTCTCTAGATTGGGTGT+TGG 0.538747 5_3:-66667199 Msa0826320:intron
ATGAGAGACCTGAGAACAGC+CGG 0.541466 5_3:-66667425 Msa0826320:CDS
TAGAACACTTGCAGCTAACA+AGG 0.541533 5_3:-66666236 Msa0826320:intron
GTGGTTGGGAAAAGATTAGC+TGG 0.541972 5_3:-66664746 Msa0826320:CDS
ATTGACACAGTTGACGGATG+TGG 0.544827 5_3:+66667565 None:intergenic
TGGCGGGGATATTGGATGAT+CGG 0.555756 5_3:+66667585 None:intergenic
TTTAAGGTGGAATTCACGGA+TGG 0.557502 5_3:-66665196 Msa0826320:CDS
AATTGGATACTCGCAAATAC+CGG 0.560870 5_3:+66665252 None:intergenic
TCATTGCGTCAAAGTTGAGA+AGG 0.566953 5_3:-66664657 Msa0826320:CDS
GATCGCCGCAGCTGACGGCA+TGG 0.567039 5_3:+66667674 None:intergenic
AGCATCAAACGTACATCGAA+AGG 0.568398 5_3:+66664482 None:intergenic
TAACGGATCGCCGCAGCTGA+CGG 0.568917 5_3:+66667669 None:intergenic
TACCGGAGGGAGGCAATGAA+GGG 0.573825 5_3:+66667706 None:intergenic
GAGAGTTCCAGAGACGGTAG+AGG 0.574827 5_3:+66667728 None:intergenic
TCCGTTGGAGCATGTTCAAG+AGG 0.576223 5_3:-66666299 Msa0826320:CDS
AAGAAGATTGAGACGACAGA+TGG 0.579468 5_3:+66667629 None:intergenic
AAAAGATTAGCTGGCAGCGT+GGG 0.580966 5_3:-66664737 Msa0826320:CDS
TGTAGAAGCCATTGCTTGAG+TGG 0.583493 5_3:-66664827 Msa0826320:intron
CACAGTTGACGGATGTGGCG+GGG 0.583831 5_3:+66667570 None:intergenic
ATCTTCCATGCCGTCAGCTG+CGG 0.586670 5_3:-66667679 Msa0826320:five_prime_UTR
ACACCAAGTAGCAAAGTGGT+TGG 0.586766 5_3:-66664761 Msa0826320:CDS
AAAACCATCAAAATTAGGGT+TGG 0.590286 5_3:+66667897 None:intergenic
CTCAGGTCTCTCATCGACAT+CGG 0.590904 5_3:+66667434 None:intergenic
TTAAGGTGGAATTCACGGAT+GGG 0.591193 5_3:-66665195 Msa0826320:CDS
GACACAGTTGACGGATGTGG+CGG 0.593385 5_3:+66667568 None:intergenic
TGGAATCACCACTCAAGCAA+TGG 0.595255 5_3:+66664819 None:intergenic
AACGTGGAAGAAACTGAAGT+TGG 0.595322 5_3:-66664710 Msa0826320:CDS
AACTTTAGGAACATTAATTG+CGG 0.604320 5_3:-66667143 Msa0826320:CDS
AAAACAATCCTTAGACGATG+TGG 0.607051 5_3:+66664580 None:intergenic
AAGATTGAGACGACAGATGG+AGG 0.608758 5_3:+66667632 None:intergenic
AGCTTCTCTTGATAGCATAG+TGG 0.608989 5_3:+66665152 None:intergenic
CCGTTGGAGCATGTTCAAGA+GGG 0.612238 5_3:-66666298 Msa0826320:CDS
TTTAGGAACATTAATTGCGG+TGG 0.612421 5_3:-66667140 Msa0826320:intron
TGAAGGGAGAGTTCCAGAGA+CGG 0.614015 5_3:+66667722 None:intergenic
GTATTGGCATTGATGTTGTA+CGG 0.614046 5_3:-66667503 Msa0826320:exon
GTTCCAGAGACGGTAGAGGT+GGG 0.617227 5_3:+66667732 None:intergenic
ATCTATACAGATGCATCCAA+CGG 0.620179 5_3:+66667856 None:intergenic
AGGCAATGACGGGATTGTAA+CGG 0.626022 5_3:+66667652 None:intergenic
TGCTGACAAGATTTGCAACA+AGG 0.629992 5_3:-66665001 Msa0826320:intron
GTACAACATCAATGCCAATA+CGG 0.633208 5_3:+66667505 None:intergenic
AAATACACCAAGTAGCAAAG+TGG 0.642147 5_3:-66664765 Msa0826320:CDS
TGGCAGCGTGGGTTCGAACG+TGG 0.648721 5_3:-66664726 Msa0826320:CDS
ATGGAAGATGAGATACCGGA+GGG 0.655985 5_3:+66667693 None:intergenic
TAAATGGATCAATCCATACA+CGG 0.666552 5_3:+66664524 None:intergenic
GAAGATGAGATACCGGAGGG+AGG 0.668843 5_3:+66667696 None:intergenic
AACTGAGAGCAAATCAAACG+AGG 0.670294 5_3:-66664792 Msa0826320:CDS
GAAAAGATTAGCTGGCAGCG+TGG 0.680628 5_3:-66664738 Msa0826320:CDS
CATGGAAGATGAGATACCGG+AGG 0.681913 5_3:+66667692 None:intergenic
CGACATCGGACATATTTCGG+TGG 0.691640 5_3:+66667448 None:intergenic
AAACCTAGCGTTCATCAGCG+AGG 0.714258 5_3:+66667388 None:intergenic
GGTTGCCGGAGAATTAGCGG+CGG 0.756792 5_3:+66667526 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAATTATATTACTTTTTTT+CGG - chr5_3:66665445-66665464 Msa0826320:intron 10.0%
!! AATATAATTCATAGATCAAA+AGG + chr5_3:66665436-66665455 None:intergenic 15.0%
!!! TATTTTTCATCTATTGATAA+TGG - chr5_3:66665877-66665896 Msa0826320:intron 15.0%
!! TTTATATAATCATAGATAGA+AGG + chr5_3:66666264-66666283 None:intergenic 15.0%
!!! TCAAAATTATAATAATGGAA+AGG + chr5_3:66666439-66666458 None:intergenic 15.0%
!!! TTGTAAGCTTTAAATAATTA+TGG - chr5_3:66666862-66666881 Msa0826320:intron 15.0%
!!! TGTAAGCTTTAAATAATTAT+GGG - chr5_3:66666863-66666882 Msa0826320:intron 15.0%
!!! GATTTTTTGTTTTTTGTTTT+TGG - chr5_3:66667805-66667824 Msa0826320:five_prime_UTR 15.0%
!! TAAACAAAACAACATATAAA+TGG + chr5_3:66667852-66667871 None:intergenic 15.0%
!! ACTTAAAATTGAAAGACATT+GGG + chr5_3:66664547-66664566 None:intergenic 20.0%
!! AACATGATAAAAGAATAGAA+GGG + chr5_3:66665060-66665079 None:intergenic 20.0%
!! AAACATGATAAAAGAATAGA+AGG + chr5_3:66665061-66665080 None:intergenic 20.0%
!!! TTTATTCCTAATTTTCTAGA+AGG - chr5_3:66665078-66665097 Msa0826320:intron 20.0%
!! TTATTTACTTTCTCTAGATT+GGG - chr5_3:66665152-66665171 Msa0826320:CDS 20.0%
!! ATAGAAATAAACACAAAGAA+TGG + chr5_3:66665388-66665407 None:intergenic 20.0%
!!! AATTTCTTTTGATTAAGTCA+TGG - chr5_3:66665697-66665716 Msa0826320:intron 20.0%
!! TTATATTTGCATCATATCAA+AGG + chr5_3:66665944-66665963 None:intergenic 20.0%
!! TAACTCACTATAATTTACTT+TGG - chr5_3:66665961-66665980 Msa0826320:intron 20.0%
!! ATATTAGAAAGAGTTAAGTT+TGG + chr5_3:66666005-66666024 None:intergenic 20.0%
!! AAAATACACAGACATTTAAT+TGG + chr5_3:66666214-66666233 None:intergenic 20.0%
!!! ATTTGGTTACAATCATTTTA+CGG - chr5_3:66666593-66666612 Msa0826320:intron 20.0%
!! CAAAAAAAAAACGTCAAATT+AGG + chr5_3:66666628-66666647 None:intergenic 20.0%
!!! AGTGATAATATACATGATTT+TGG + chr5_3:66666687-66666706 None:intergenic 20.0%
!!! CATCTATCTTTTGATTTATT+AGG - chr5_3:66666747-66666766 Msa0826320:intron 20.0%
!!! GTTAATGTTACTGATTTTTA+AGG + chr5_3:66666811-66666830 None:intergenic 20.0%
!!! TTGTTTTCTAAATTCAAATG+TGG + chr5_3:66667037-66667056 None:intergenic 20.0%
!! TTGAATTTAGAAAACAAAGA+AGG - chr5_3:66667040-66667059 Msa0826320:intron 20.0%
!!! GTTTGATGCTTTTATATTAA+TGG - chr5_3:66667888-66667907 Msa0826320:five_prime_UTR 20.0%
!! AGAATAAAAAGTAGAAAACA+TGG + chr5_3:66667916-66667935 None:intergenic 20.0%
! GACTTAAAATTGAAAGACAT+TGG + chr5_3:66664548-66664567 None:intergenic 25.0%
!!! ATTTTAAGTCAGAACTACAT+TGG - chr5_3:66664558-66664577 Msa0826320:three_prime_UTR 25.0%
!! AAAACTCCTTCTAGAAAATT+AGG + chr5_3:66665087-66665106 None:intergenic 25.0%
!! TTTTATGCAGTGATGATTTA+TGG - chr5_3:66665104-66665123 Msa0826320:CDS 25.0%
! GTTATTTACTTTCTCTAGAT+TGG - chr5_3:66665151-66665170 Msa0826320:CDS 25.0%
!! AACTTTAGGAACATTAATTG+CGG - chr5_3:66665214-66665233 Msa0826320:CDS 25.0%
!!! TATATTACTTTTTTTCGGCT+AGG - chr5_3:66665450-66665469 Msa0826320:intron 25.0%
! TATAATCATAGATAGAAGGA+TGG + chr5_3:66666260-66666279 None:intergenic 25.0%
! AATCAAAAGATAGATGAGTT+AGG + chr5_3:66666743-66666762 None:intergenic 25.0%
!!! TAATTGTTGTTTAATTGTGC+AGG - chr5_3:66667079-66667098 Msa0826320:intron 25.0%
!!! AATTGTTGTTTAATTGTGCA+GGG - chr5_3:66667080-66667099 Msa0826320:intron 25.0%
!! TAGTTGTACAAGATTTTGTT+CGG - chr5_3:66667279-66667298 Msa0826320:intron 25.0%
!!! CGAGGTTTTAAGTTTTTAAT+TGG - chr5_3:66667482-66667501 Msa0826320:CDS 25.0%
! CATTAAAACAATGATACTAC+TGG + chr5_3:66667736-66667755 None:intergenic 25.0%
AAAACCATCAAAATTAGGGT+TGG + chr5_3:66664463-66664482 None:intergenic 30.0%
!! AACCCTAATTTTGATGGTTT+TGG - chr5_3:66664462-66664481 Msa0826320:three_prime_UTR 30.0%
AGCCAAAACCATCAAAATTA+GGG + chr5_3:66664467-66664486 None:intergenic 30.0%
TAATCTCCATTCATCTCATT+TGG - chr5_3:66665009-66665028 Msa0826320:intron 30.0%
TGAAGTCTCTCTAAGTAATA+GGG + chr5_3:66665570-66665589 None:intergenic 30.0%
ATGAAGTCTCTCTAAGTAAT+AGG + chr5_3:66665571-66665590 None:intergenic 30.0%
AAAATGGTTTCATCATAACG+TGG + chr5_3:66665603-66665622 None:intergenic 30.0%
!!! AAACCATTTTTAGTTATGCC+AGG - chr5_3:66665613-66665632 Msa0826320:intron 30.0%
AGAAGTTCCTCAATGATTTA+AGG - chr5_3:66665763-66665782 Msa0826320:intron 30.0%
TTTAATAATTGCAGTTCCGT+TGG - chr5_3:66666043-66666062 Msa0826320:intron 30.0%
GCAAAACACTGATATTCAAT+TGG + chr5_3:66666152-66666171 None:intergenic 30.0%
GATTCAATGAAGAACAATCA+GGG + chr5_3:66666180-66666199 None:intergenic 30.0%
TGATTCAATGAAGAACAATC+AGG + chr5_3:66666181-66666200 None:intergenic 30.0%
AAACTGATTGATGAGAAGTA+TGG + chr5_3:66666402-66666421 None:intergenic 30.0%
!!! TTTGTTTGTTTTAGCACCAT+TGG - chr5_3:66666464-66666483 Msa0826320:intron 30.0%
AATCTAATCTAACTCGGATA+CGG + chr5_3:66666499-66666518 None:intergenic 30.0%
ATTCGCAATCTAATCTAACT+CGG + chr5_3:66666505-66666524 None:intergenic 30.0%
! TTGATTTATTAGGTGCTACA+CGG - chr5_3:66666757-66666776 Msa0826320:intron 30.0%
!!! ACACAATCAGGTAGTTTTAT+GGG - chr5_3:66666986-66667005 Msa0826320:intron 30.0%
AACAACAATTATTGCCACTA+TGG + chr5_3:66667070-66667089 None:intergenic 30.0%
ACAAAAACATCCAGATCAAT+TGG + chr5_3:66667125-66667144 None:intergenic 30.0%
!! ATTGATCTGGATGTTTTTGT+TGG - chr5_3:66667125-66667144 Msa0826320:intron 30.0%
!! TTGATCTGGATGTTTTTGTT+GGG - chr5_3:66667126-66667145 Msa0826320:intron 30.0%
! TGGGAAACAAATGTTGTTTA+AGG - chr5_3:66667145-66667164 Msa0826320:CDS 30.0%
! GAAACAAATGTTGTTTAAGG+TGG - chr5_3:66667148-66667167 Msa0826320:CDS 30.0%
ATGGGAACTTAAAGCATAAT+TGG - chr5_3:66667180-66667199 Msa0826320:CDS 30.0%
! GTACAACTACCTTAATTTTG+TGG + chr5_3:66667268-66667287 None:intergenic 30.0%
GAAAACGTAACATATACTTG+TGG + chr5_3:66667460-66667479 None:intergenic 30.0%
! TATATGTTACGTTTTCAACG+AGG - chr5_3:66667464-66667483 Msa0826320:CDS 30.0%
TAAATGGATCAATCCATACA+CGG + chr5_3:66667836-66667855 None:intergenic 30.0%
GAGCCAAAACCATCAAAATT+AGG + chr5_3:66664468-66664487 None:intergenic 35.0%
!!! GTTTTGGCTCCAAACTATTA+TGG - chr5_3:66664478-66664497 Msa0826320:three_prime_UTR 35.0%
ATCTATACAGATGCATCCAA+CGG + chr5_3:66664504-66664523 None:intergenic 35.0%
GAATATTGGTGCGAGTTTAT+TGG + chr5_3:66664604-66664623 None:intergenic 35.0%
AGAGAAATTGACACAGTTGA+CGG + chr5_3:66664801-66664820 None:intergenic 35.0%
GTACAACATCAATGCCAATA+CGG + chr5_3:66664855-66664874 None:intergenic 35.0%
!! GTATTGGCATTGATGTTGTA+CGG - chr5_3:66664854-66664873 Msa0826320:intron 35.0%
GACATACCAAATGAGATGAA+TGG + chr5_3:66665018-66665037 None:intergenic 35.0%
!! TGTGGTTGATGAAACTTATG+TGG - chr5_3:66665128-66665147 Msa0826320:CDS 35.0%
!! GTGGTTGATGAAACTTATGT+GGG - chr5_3:66665129-66665148 Msa0826320:CDS 35.0%
TTTAGGAACATTAATTGCGG+TGG - chr5_3:66665217-66665236 Msa0826320:CDS 35.0%
AATTCACACGTTGAAGATGA+CGG - chr5_3:66665477-66665496 Msa0826320:intron 35.0%
ATAGGGAAAAGTGAAATACG+TGG + chr5_3:66665553-66665572 None:intergenic 35.0%
AGTCCTGGCATAACTAAAAA+TGG + chr5_3:66665619-66665638 None:intergenic 35.0%
CAATGTTCAAATTGAAGTCC+TGG + chr5_3:66665634-66665653 None:intergenic 35.0%
!! CATGGTTGAATTACTGATTC+AGG - chr5_3:66665715-66665734 Msa0826320:intron 35.0%
TCACTTGCCTTAAATCATTG+AGG + chr5_3:66665773-66665792 None:intergenic 35.0%
TTCGCATCTAATCATGTAGA+TGG - chr5_3:66665831-66665850 Msa0826320:intron 35.0%
!! TCATGTAGATGGTTGATAGT+TGG - chr5_3:66665842-66665861 Msa0826320:intron 35.0%
TAGCACAGATGAGAATAGAT+GGG + chr5_3:66666324-66666343 None:intergenic 35.0%
ATAGCACAGATGAGAATAGA+TGG + chr5_3:66666325-66666344 None:intergenic 35.0%
ATCTATTCTCATCTGTGCTA+TGG - chr5_3:66666324-66666343 Msa0826320:intron 35.0%
TATACCTGTCAAACAATGGT+AGG + chr5_3:66666377-66666396 None:intergenic 35.0%
GGTTTATACCTGTCAAACAA+TGG + chr5_3:66666381-66666400 None:intergenic 35.0%
ATCTAATCTAACTCGGATAC+GGG + chr5_3:66666498-66666517 None:intergenic 35.0%
GAACTTGATGTTGAATACGT+AGG - chr5_3:66666558-66666577 Msa0826320:intron 35.0%
! GTAGGTTATTGCTGCTTATT+TGG - chr5_3:66666576-66666595 Msa0826320:intron 35.0%
CAACGTTTGTTTGTATGACA+GGG - chr5_3:66666648-66666667 Msa0826320:intron 35.0%
!! CACACAATCAGGTAGTTTTA+TGG - chr5_3:66666985-66667004 Msa0826320:intron 35.0%
AATTGGATACTCGCAAATAC+CGG + chr5_3:66667108-66667127 None:intergenic 35.0%
! GTTGTTTAAGGTGGAATTCA+CGG - chr5_3:66667157-66667176 Msa0826320:CDS 35.0%
TGTGACTGTCCACAAAATTA+AGG - chr5_3:66667256-66667275 Msa0826320:intron 35.0%
TTGCATATATCCTGAGATGT+TGG - chr5_3:66667392-66667411 Msa0826320:CDS 35.0%
! ATATCCTGAGATGTTGGTTA+TGG - chr5_3:66667398-66667417 Msa0826320:CDS 35.0%
!!! TGATTTGCTCTCAGTTTTAG+TGG + chr5_3:66667561-66667580 None:intergenic 35.0%
AAATACACCAAGTAGCAAAG+TGG - chr5_3:66667592-66667611 Msa0826320:five_prime_UTR 35.0%
AAAACAATCCTTAGACGATG+TGG + chr5_3:66667780-66667799 None:intergenic 35.0%
! TGTGCCAACCCTAATTTTGA+TGG - chr5_3:66664456-66664475 Msa0826320:three_prime_UTR 40.0%
ATCCAACGGCCATAATAGTT+TGG + chr5_3:66664490-66664509 None:intergenic 40.0%
TTGGGAACAGTTGGATTGTA+TGG + chr5_3:66664529-66664548 None:intergenic 40.0%
TGAAAGACATTGGGAACAGT+TGG + chr5_3:66664538-66664557 None:intergenic 40.0%
TGGTGCGAGTTTATTGGATT+AGG + chr5_3:66664598-66664617 None:intergenic 40.0%
AAGAAGATTGAGACGACAGA+TGG + chr5_3:66664731-66664750 None:intergenic 40.0%
ATCTTCTTCACCAAACTCTC+TGG - chr5_3:66664743-66664762 Msa0826320:CDS 40.0%
CATCGACATCGGACATATTT+CGG + chr5_3:66664915-66664934 None:intergenic 40.0%
AACGCTAGGTTTGAGGAATT+AGG - chr5_3:66664980-66664999 Msa0826320:CDS 40.0%
GCAGTGATGATTTATGGTTG+TGG - chr5_3:66665110-66665129 Msa0826320:CDS 40.0%
ACTTTCTCTAGATTGGGTGT+TGG - chr5_3:66665158-66665177 Msa0826320:CDS 40.0%
GAGATTGTTGCAGAGAAGAA+AGG - chr5_3:66665183-66665202 Msa0826320:CDS 40.0%
!!! GCGGTGGTATTTTTTTGTAG+CGG - chr5_3:66665233-66665252 Msa0826320:CDS 40.0%
ATTAGGATTCAAGCCATTGC+AGG + chr5_3:66665290-66665309 None:intergenic 40.0%
GAGAGCGAGAAACAAACATT+AGG + chr5_3:66665307-66665326 None:intergenic 40.0%
GAAAAGTGAAATACGTGGAG+GGG + chr5_3:66665548-66665567 None:intergenic 40.0%
GGAAAAGTGAAATACGTGGA+GGG + chr5_3:66665549-66665568 None:intergenic 40.0%
GGCAAGTGAAGAAGAAATGA+TGG - chr5_3:66665784-66665803 Msa0826320:intron 40.0%
TAGAAGCTTATGCACGAAGA+AGG - chr5_3:66665908-66665927 Msa0826320:intron 40.0%
TAGAACACTTGCAGCTAACA+AGG - chr5_3:66666121-66666140 Msa0826320:intron 40.0%
! TCTGTGTATTTTCCCATGCA+AGG - chr5_3:66666222-66666241 Msa0826320:intron 40.0%
AATGGTAGGACATCAATGCT+TGG + chr5_3:66666363-66666382 None:intergenic 40.0%
ATGTCCTACCATTGTTTGAC+AGG - chr5_3:66666370-66666389 Msa0826320:intron 40.0%
TCTAATCTAACTCGGATACG+GGG + chr5_3:66666497-66666516 None:intergenic 40.0%
CATCAAGTACAGGACATATG+TGG - chr5_3:66666533-66666552 Msa0826320:intron 40.0%
GCAACGTTTGTTTGTATGAC+AGG - chr5_3:66666647-66666666 Msa0826320:intron 40.0%
TACACTTGAGTCACACAATC+AGG - chr5_3:66666974-66666993 Msa0826320:intron 40.0%
TGTTTAATTGTGCAGGGAAC+CGG - chr5_3:66667086-66667105 Msa0826320:intron 40.0%
TTGCGAGTATCCAATTGATC+TGG - chr5_3:66667112-66667131 Msa0826320:intron 40.0%
! TTTAAGGTGGAATTCACGGA+TGG - chr5_3:66667161-66667180 Msa0826320:CDS 40.0%
TTAAGGTGGAATTCACGGAT+GGG - chr5_3:66667162-66667181 Msa0826320:CDS 40.0%
! AGCTTCTCTTGATAGCATAG+TGG + chr5_3:66667208-66667227 None:intergenic 40.0%
TGCTGACAAGATTTGCAACA+AGG - chr5_3:66667356-66667375 Msa0826320:CDS 40.0%
ATTGCCATAACCAACATCTC+AGG + chr5_3:66667405-66667424 None:intergenic 40.0%
CGATGACAACATACATCAAG+TGG + chr5_3:66667435-66667454 None:intergenic 40.0%
AACTGAGAGCAAATCAAACG+AGG - chr5_3:66667565-66667584 Msa0826320:five_prime_UTR 40.0%
AACGTGGAAGAAACTGAAGT+TGG - chr5_3:66667647-66667666 Msa0826320:five_prime_UTR 40.0%
TTGACGCAATGAACTTTGAC+AGG + chr5_3:66667692-66667711 None:intergenic 40.0%
TCATTGCGTCAAAGTTGAGA+AGG - chr5_3:66667700-66667719 Msa0826320:five_prime_UTR 40.0%
!!! GTTTTTGGTGTTTCCGTGTA+TGG - chr5_3:66667820-66667839 Msa0826320:five_prime_UTR 40.0%
AGCATCAAACGTACATCGAA+AGG + chr5_3:66667878-66667897 None:intergenic 40.0%
CTCCAAACTATTATGGCCGT+TGG - chr5_3:66664485-66664504 Msa0826320:three_prime_UTR 45.0%
ATGGAAGATGAGATACCGGA+GGG + chr5_3:66664667-66664686 None:intergenic 45.0%
! AGGCAATGACGGGATTGTAA+CGG + chr5_3:66664708-66664727 None:intergenic 45.0%
AAGATTGAGACGACAGATGG+AGG + chr5_3:66664728-66664747 None:intergenic 45.0%
TCGGAATGATCCAGAGAGTT+TGG + chr5_3:66664756-66664775 None:intergenic 45.0%
! ATTGACACAGTTGACGGATG+TGG + chr5_3:66664795-66664814 None:intergenic 45.0%
TAATTCTCCGGCAACCGTAT+TGG - chr5_3:66664838-66664857 Msa0826320:intron 45.0%
ATCAATGCCAATACGGTTGC+CGG + chr5_3:66664848-66664867 None:intergenic 45.0%
TTCGGTGGTTACATCAGTTG+CGG + chr5_3:66664897-66664916 None:intergenic 45.0%
!! GAAAGGCTTGCTGCAACTTT+AGG - chr5_3:66665200-66665219 Msa0826320:CDS 45.0%
! ATGGTGGTGATTTCCTGCAA+TGG - chr5_3:66665274-66665293 Msa0826320:intron 45.0%
GGGAAAAGTGAAATACGTGG+AGG + chr5_3:66665550-66665569 None:intergenic 45.0%
! TGCACGAAGAAGGTTTTCGA+AGG - chr5_3:66665918-66665937 Msa0826320:intron 45.0%
! ATTTTCCCATGCAAGGCTGT+AGG - chr5_3:66666229-66666248 Msa0826320:intron 45.0%
TCATACCTACAGCCTTGCAT+GGG + chr5_3:66666237-66666256 None:intergenic 45.0%
!! ATAGATGGGCTACACATGCA+TGG + chr5_3:66666310-66666329 None:intergenic 45.0%
ATGAGTTGCGCATCAAGTAC+AGG - chr5_3:66666523-66666542 Msa0826320:intron 45.0%
CAAGTACAGGACATATGTGG+TGG - chr5_3:66666536-66666555 Msa0826320:intron 45.0%
!! ACAAAGAAGGCGTTCCATAG+TGG - chr5_3:66667053-66667072 Msa0826320:intron 45.0%
TGTAGAAGCCATTGCTTGAG+TGG - chr5_3:66667530-66667549 Msa0826320:five_prime_UTR 45.0%
TGGAATCACCACTCAAGCAA+TGG + chr5_3:66667541-66667560 None:intergenic 45.0%
ACACCAAGTAGCAAAGTGGT+TGG - chr5_3:66667596-66667615 Msa0826320:five_prime_UTR 45.0%
CACCAAGTAGCAAAGTGGTT+GGG - chr5_3:66667597-66667616 Msa0826320:five_prime_UTR 45.0%
TTCCCAACCACTTTGCTACT+TGG + chr5_3:66667602-66667621 None:intergenic 45.0%
GTGGTTGGGAAAAGATTAGC+TGG - chr5_3:66667611-66667630 Msa0826320:five_prime_UTR 45.0%
! AAAAGATTAGCTGGCAGCGT+GGG - chr5_3:66667620-66667639 Msa0826320:five_prime_UTR 45.0%
!! AAAAATCAAAATTATAATAA+TGG + chr5_3:66666444-66666463 None:intergenic 5.0%
CGGTAGAGGTGGGAGAATAT+TGG + chr5_3:66664618-66664637 None:intergenic 50.0%
TGAAGGGAGAGTTCCAGAGA+CGG + chr5_3:66664638-66664657 None:intergenic 50.0%
CATGGAAGATGAGATACCGG+AGG + chr5_3:66664668-66664687 None:intergenic 50.0%
CGGCATGGAAGATGAGATAC+CGG + chr5_3:66664671-66664690 None:intergenic 50.0%
ACGACAGATGGAGGCAATGA+CGG + chr5_3:66664719-66664738 None:intergenic 50.0%
TGGCGGGGATATTGGATGAT+CGG + chr5_3:66664775-66664794 None:intergenic 50.0%
TACGGTTGCCGGAGAATTAG+CGG + chr5_3:66664837-66664856 None:intergenic 50.0%
TCAGTTGCGGCTGATGAATC+TGG + chr5_3:66664884-66664903 None:intergenic 50.0%
CGACATCGGACATATTTCGG+TGG + chr5_3:66664912-66664931 None:intergenic 50.0%
CTCAGGTCTCTCATCGACAT+CGG + chr5_3:66664926-66664945 None:intergenic 50.0%
ATGAGAGACCTGAGAACAGC+CGG - chr5_3:66664932-66664951 Msa0826320:intron 50.0%
CGAGGTTGCAAGTGTAGATC+CGG + chr5_3:66664954-66664973 None:intergenic 50.0%
AAACCTAGCGTTCATCAGCG+AGG + chr5_3:66664972-66664991 None:intergenic 50.0%
GCTGATGAACGCTAGGTTTG+AGG - chr5_3:66664973-66664992 Msa0826320:CDS 50.0%
!!! GCTCACGCTGATGGTTTTGA+TGG - chr5_3:66665255-66665274 Msa0826320:CDS 50.0%
!!! CACGCTGATGGTTTTGATGG+TGG - chr5_3:66665258-66665277 Msa0826320:CDS 50.0%
TCCGTTGGAGCATGTTCAAG+AGG - chr5_3:66666058-66666077 Msa0826320:intron 50.0%
CCCTCTTGAACATGCTCCAA+CGG + chr5_3:66666062-66666081 None:intergenic 50.0%
CCGTTGGAGCATGTTCAAGA+GGG - chr5_3:66666059-66666078 Msa0826320:intron 50.0%
GTCATACCTACAGCCTTGCA+TGG + chr5_3:66666238-66666257 None:intergenic 50.0%
TCTAACTCGGATACGGGGTT+CGG + chr5_3:66666492-66666511 None:intergenic 50.0%
CGATGAATATCAGACGCGCT+AGG - chr5_3:66666904-66666923 Msa0826320:intron 50.0%
! GAAAAGATTAGCTGGCAGCG+TGG - chr5_3:66667619-66667638 Msa0826320:five_prime_UTR 50.0%
! GCGTTGATCCACATCGTCTA+AGG - chr5_3:66667769-66667788 Msa0826320:five_prime_UTR 50.0%
GTTCCAGAGACGGTAGAGGT+GGG + chr5_3:66664628-66664647 None:intergenic 55.0%
AGTTCCAGAGACGGTAGAGG+TGG + chr5_3:66664629-66664648 None:intergenic 55.0%
GAGAGTTCCAGAGACGGTAG+AGG + chr5_3:66664632-66664651 None:intergenic 55.0%
TACCGGAGGGAGGCAATGAA+GGG + chr5_3:66664654-66664673 None:intergenic 55.0%
ATACCGGAGGGAGGCAATGA+AGG + chr5_3:66664655-66664674 None:intergenic 55.0%
GAAGATGAGATACCGGAGGG+AGG + chr5_3:66664664-66664683 None:intergenic 55.0%
ATCTTCCATGCCGTCAGCTG+CGG - chr5_3:66664678-66664697 Msa0826320:CDS 55.0%
CGACAGATGGAGGCAATGAC+GGG + chr5_3:66664718-66664737 None:intergenic 55.0%
! ACACAGTTGACGGATGTGGC+GGG + chr5_3:66664791-66664810 None:intergenic 55.0%
! GACACAGTTGACGGATGTGG+CGG + chr5_3:66664792-66664811 None:intergenic 55.0%
TAACGCCGCCGCTAATTCTC+CGG - chr5_3:66664826-66664845 Msa0826320:intron 55.0%
GTGTAGATCCGGCTGTTCTC+AGG + chr5_3:66664943-66664962 None:intergenic 55.0%
CAACCTCGCTGATGAACGCT+AGG - chr5_3:66664966-66664985 Msa0826320:intron 55.0%
TTTGTAGCGGCTCACGCTGA+TGG - chr5_3:66665246-66665265 Msa0826320:CDS 55.0%
GATACGGGGTTCGGATCCAA+TGG + chr5_3:66666483-66666502 None:intergenic 55.0%
TCTCCCACCTCTACCGTCTC+TGG - chr5_3:66664622-66664641 Msa0826320:three_prime_UTR 60.0%
CTCCCTTCATTGCCTCCCTC+CGG - chr5_3:66664649-66664668 Msa0826320:exon 60.0%
! TAACGGATCGCCGCAGCTGA+CGG + chr5_3:66664691-66664710 None:intergenic 60.0%
! GACGGATGTGGCGGGGATAT+TGG + chr5_3:66664783-66664802 None:intergenic 60.0%
! CACAGTTGACGGATGTGGCG+GGG + chr5_3:66664790-66664809 None:intergenic 60.0%
GGAGAATTAGCGGCGGCGTT+AGG + chr5_3:66664827-66664846 None:intergenic 60.0%
GGTTGCCGGAGAATTAGCGG+CGG + chr5_3:66664834-66664853 None:intergenic 60.0%
TGGCAGCGTGGGTTCGAACG+TGG - chr5_3:66667631-66667650 Msa0826320:five_prime_UTR 65.0%
GATCGCCGCAGCTGACGGCA+TGG + chr5_3:66664686-66664705 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 66664439 66667940 66664439 ID=Msa0826320;Name=Msa0826320
chr5_3 mRNA 66664439 66667940 66664439 ID=Msa0826320-mRNA-1;Parent=Msa0826320;Name=Msa0826320-mRNA-1;_AED=0.27;_eAED=0.27;_QI=427|0.4|0.66|1|1|1|6|213|244
chr5_3 exon 66664439 66664844 66664439 ID=Msa0826320-mRNA-1:exon:20921;Parent=Msa0826320-mRNA-1
chr5_3 exon 66664970 66665013 66664970 ID=Msa0826320-mRNA-1:exon:20920;Parent=Msa0826320-mRNA-1
chr5_3 exon 66665102 66665278 66665102 ID=Msa0826320-mRNA-1:exon:20919;Parent=Msa0826320-mRNA-1
chr5_3 exon 66666237 66666322 66666237 ID=Msa0826320-mRNA-1:exon:20918;Parent=Msa0826320-mRNA-1
chr5_3 exon 66667141 66667210 66667141 ID=Msa0826320-mRNA-1:exon:20917;Parent=Msa0826320-mRNA-1
chr5_3 exon 66667349 66667940 66667349 ID=Msa0826320-mRNA-1:exon:20916;Parent=Msa0826320-mRNA-1
chr5_3 five_prime_UTR 66667514 66667940 66667514 ID=Msa0826320-mRNA-1:five_prime_utr;Parent=Msa0826320-mRNA-1
chr5_3 CDS 66667349 66667513 66667349 ID=Msa0826320-mRNA-1:cds;Parent=Msa0826320-mRNA-1
chr5_3 CDS 66667141 66667210 66667141 ID=Msa0826320-mRNA-1:cds;Parent=Msa0826320-mRNA-1
chr5_3 CDS 66666237 66666322 66666237 ID=Msa0826320-mRNA-1:cds;Parent=Msa0826320-mRNA-1
chr5_3 CDS 66665102 66665278 66665102 ID=Msa0826320-mRNA-1:cds;Parent=Msa0826320-mRNA-1
chr5_3 CDS 66664970 66665013 66664970 ID=Msa0826320-mRNA-1:cds;Parent=Msa0826320-mRNA-1
chr5_3 CDS 66664652 66664844 66664652 ID=Msa0826320-mRNA-1:cds;Parent=Msa0826320-mRNA-1
chr5_3 three_prime_UTR 66664439 66664651 66664439 ID=Msa0826320-mRNA-1:three_prime_utr;Parent=Msa0826320-mRNA-1
Gene Sequence

>Msa0826320

ATGTTGTACGGCTGTCCAGATTCATCAGCCGCAACTGATGTAACCACCGAAATATGTCCGATGTCGATGAGAGACCTGAGAACAGCCGGATCTACACTTGCAACCTCGCTGATGAACGCTAGGTTTGAGGAATTAGGTCTCTTTAATCTCCATTCATCTCATTTGATTGGGTGTTGGTCGAGATTGTTGCAGAGAAGAAAGGCTTGCTGCAACTTTAGGAACATTAATTGCGGTGTTCCGTTGGAGCATGTTCAAGAGGGAACTAATGATGCTTATCTTTATGATATGAAGATCAAATTTAGAACACTTGCAGCTAACAAGGGAACCGGTATTTGCGAGTATCCAATTGATCTGGATGTTTTTGTTGGGAAACAAATGTTGTTTAAGGTGGAATTCACGGATGGGAACTTAAAGCATAATTGGCGCCACTATGCTATCAAGAGAAGCTCTGATGACGCGAATCTTATTACTCAATTTGTGACTGTCCACAAAATTAAGATTTGCAACAAGGACAATGAAGCTGATTGCATATATCCTGAGATAAGCCATTGCTTGAGTGGTGATTCCACTAAAACTGAGAGCAAATCAAACGAGGATGAAAATACACCAAGTAGCAAAGTGGTTGGGAAAAGATTAGCTGGCAGCGTGGGTTCGAACGTGGAAGAAACTGAAGTTGGTGAAAGATCGATCAACAAACCTGTCAAAGTTCATTGCGTCAAAGTTGAGAAGGATTAG
Protein sequence

>Msa0826320

MLYGCPDSSAATDVTTEICPMSMRDLRTAGSTLATSLMNARFEELGLFNLHSSHLIGCWSRLLQRRKACCNFRNINCGVPLEHVQEGTNDAYLYDMKIKFRTLAANKGTGICEYPIDLDVFVGKQMLFKVEFTDGNLKHNWRHYAIKRSSDDANLITQFVTVHKIKICNKDNEADCIYPEISHCLSGDSTKTESKSNEDENTPSSKVVGKRLAGSVGSNVEETEVGERSINKPVKVHCVKVEKD*