AlfalfaGEDB | Msa0539050

M. sativa cultivar zhongmu-4 / Msa0539050

KEGG: -
GO: -
PFAM: PF03004.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0539050	GAU48222.1	72.754	345	62	3	62	378	89	429	1.26e-177	511

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0539050	A0A2Z6PEX1	72.754	345	62	3	62	378	89	429	6.02e-178	511

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0008840	Msa0539050	0.803949	2.730113e-49	-8.615850e-47
Msa0047280	Msa0539050	0.815439	9.241649e-52	-8.615850e-47
Msa0065010	Msa0539050	0.813924	2.000911e-51	-8.615850e-47
Msa0139990	Msa0539050	0.811922	5.494314e-51	-8.615850e-47
Msa0175200	Msa0539050	0.814687	1.357025e-51	-8.615850e-47
Msa0337510	Msa0539050	0.812620	3.867685e-51	-8.615850e-47
Msa0364990	Msa0539050	0.802277	6.056542e-49	-8.615850e-47
Msa0394280	Msa0539050	0.824005	1.020416e-53	-8.615850e-47
Msa0431770	Msa0539050	0.824217	9.098816e-54	-8.615850e-47
Msa0436200	Msa0539050	0.803283	3.753183e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0539050	MtrunA17_Chr5g0414621	58.250	400	151	5	1	387	687	1083	1.72e-147	445
Msa0539050	MtrunA17_Chr3g0086081	25.253	297	203	7	93	387	8	287	1.91e-16	79.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 106 sgRNAs with CRISPR-Local

Find 278 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGTACGGCAGGAAGTTT+AGG	0.369105	4_1:+19125271	Msa0539050:CDS
AACTCATCTTTCATTTCACT+TGG	0.429996	4_1:-19125649	None:intergenic
AAGACGAGACAGACATTTGT+GGG	0.505405	4_1:+19126441	Msa0539050:CDS
AAGATGAACAACGCTACTTT+GGG	0.421905	4_1:+19125310	Msa0539050:CDS
AAGCTGACACAAACCCACCA+AGG	0.689462	4_1:-19126476	None:intergenic
AAGGAATGAAGGCAGAAGTA+TGG	0.329512	4_1:+19125928	Msa0539050:CDS
AATATCACGGGTCACAGTTT+GGG	0.329052	4_1:-19125572	None:intergenic
AATGAAAGATGAGTTATTTA+AGG	0.170608	4_1:+19125657	Msa0539050:CDS
AATGCAACAAAAGGTACGGC+AGG	0.551052	4_1:+19125262	Msa0539050:CDS
ACAAAATCATGGACTGCCTG+GGG	0.638168	4_1:+19125003	Msa0539050:five_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTCTCTTAAGGATGCTTAAT+TGG	+	chr4_1:19124786-19124805	Msa0539050:five_prime_UTR	30.0%
GGATAACAATCAAGATCATT+TGG	-	chr4_1:19124840-19124859	None:intergenic	30.0%
TTGTAATTATGAAACCCGAT+CGG	+	chr4_1:19124887-19124906	Msa0539050:five_prime_UTR	30.0%
TCTTAAATTCTTCGGTTACT+TGG	-	chr4_1:19124945-19124964	None:intergenic	30.0%
GTAACCGAAGAATTTAAGAA+TGG	+	chr4_1:19124946-19124965	Msa0539050:five_prime_UTR	30.0%
TGCTCCATTCTTAAATTCTT+CGG	-	chr4_1:19124953-19124972	None:intergenic	30.0%
GAGAAAAGTTATATCTGTTG+AGG	+	chr4_1:19125408-19125427	Msa0539050:CDS	30.0%
TCACCCTAGATATAATATCA+CGG	-	chr4_1:19125588-19125607	None:intergenic	30.0%
AACTCATCTTTCATTTCACT+TGG	-	chr4_1:19125652-19125671	None:intergenic	30.0%
TAGTTGTATTATAATGGTGC+AGG	+	chr4_1:19125743-19125762	Msa0539050:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	19124750	19126698	19124750	ID=Msa0539050;Name=Msa0539050
chr4_1	mRNA	19124750	19126698	19124750	ID=Msa0539050-mRNA-1;Parent=Msa0539050;Name=Msa0539050-mRNA-1;_AED=0.27;_eAED=0.27;_QI=317\|0.4\|0.5\|0.83\|1\|1\|6\|0\|389
chr4_1	exon	19124750	19125127	19124750	ID=Msa0539050-mRNA-1:exon:5578;Parent=Msa0539050-mRNA-1
chr4_1	exon	19125248	19125443	19125248	ID=Msa0539050-mRNA-1:exon:5579;Parent=Msa0539050-mRNA-1
chr4_1	exon	19125558	19125687	19125558	ID=Msa0539050-mRNA-1:exon:5580;Parent=Msa0539050-mRNA-1
chr4_1	exon	19125765	19126088	19125765	ID=Msa0539050-mRNA-1:exon:5581;Parent=Msa0539050-mRNA-1
chr4_1	exon	19126156	19126257	19126156	ID=Msa0539050-mRNA-1:exon:5582;Parent=Msa0539050-mRNA-1
chr4_1	exon	19126342	19126698	19126342	ID=Msa0539050-mRNA-1:exon:5583;Parent=Msa0539050-mRNA-1
chr4_1	five_prime_UTR	19124750	19125066	19124750	ID=Msa0539050-mRNA-1:five_prime_utr;Parent=Msa0539050-mRNA-1
chr4_1	CDS	19125067	19125127	19125067	ID=Msa0539050-mRNA-1:cds;Parent=Msa0539050-mRNA-1
chr4_1	CDS	19125248	19125443	19125248	ID=Msa0539050-mRNA-1:cds;Parent=Msa0539050-mRNA-1
chr4_1	CDS	19125558	19125687	19125558	ID=Msa0539050-mRNA-1:cds;Parent=Msa0539050-mRNA-1
chr4_1	CDS	19125765	19126088	19125765	ID=Msa0539050-mRNA-1:cds;Parent=Msa0539050-mRNA-1
chr4_1	CDS	19126156	19126257	19126156	ID=Msa0539050-mRNA-1:cds;Parent=Msa0539050-mRNA-1
chr4_1	CDS	19126342	19126698	19126342	ID=Msa0539050-mRNA-1:cds;Parent=Msa0539050-mRNA-1

Gene Sequence

>Msa0539050

ATGCATCTTATTTGGGAGAAACAAAATCAACAAGAAACTGATACAAATGATTCAAGAAAAGGAACTGATACAAAAAATGCAACAAAAGGTACGGCAGGAAGTTTAGGTGTTTCTAACAGTGAGAAGATGAACAACGCTACTTTGGGTTCTTTCCCACGGTCTACTGATCCACCAACTAACAACTCAACTTCAGTACCTTCAACTGAAGGATCTCATGATAGGAGAAAAGTTATATCTGTTGAGGCAGTCTCTAACCCGAGGAGGTTTGTTCCCCAAACTGTGACCCGTGATATTATATCTAGGGTGATCACGAGAATGCCTACTCCAGCTGAGAAGTGGAAAGATTATCCAAGTGAAATGAAAGATGAGTTATTTAAGGAATTTACGGGTAAATATAAGTTTACATCAGATTATGATTGTAATATGTCAAGAACCGTGTGGGAAAGAACATGCATGGACCGCTATCCTGATCATTTAAAAAATGCAAGAAAAATGGCCTTACGAGAAGTTAACTCGGTGAATTTGGTTGACACCAAGGGTCATGCACCCAAAGGAATGAAGGCAGAAGTATGGGATGGCTTGGTCGACATTTGGTTGAAACCAGAGTGGAAGAAGAAGTCTGATGCAAATCGTTGTAATAGAGCTGCCTTACCTGATGCAGTGTTGCACACAGGAGGCTCTATAAACTTTGGTGAACACAAAATAAGGATGGAGAAGGAGTTAAATCATGAAGTGTCGTATAGAGATGTGTATGACCGTGTTCATAAGAAAAAAGATGGAGAATATGTCTCTCAACGCTCAAAGAAGTTTATTGAGACATATGACACTGCCATGCTGGAAAAGTATGGAGATGATTTTTCCACTCATCCGGCAGTTGATTCTAACTTGTGGGCAGACACATTTAGTCACCATAAGACGAGACAGACATTTGTGGGTCGCAGCTTAGACCTTGGTGGGTTTGTGTCAGCTTCATCTGCAGAGTTTACTGGTCCTTCCAACATCTTAGCTACATCAGAAGAAGCTATCAAGAAAGCTGTAGATAAAGCAATGAGTTCTTTTGTACAATCACAGTTGATGCCATTATTGGAGCCAATCATATCACTGATTCAATCCATGCACAAAGCACCAATGCAAGGTGAATTCGCTGAAGGTCACATGGATGCTAGGTAA

Protein sequence

>Msa0539050

MHLIWEKQNQQETDTNDSRKGTDTKNATKGTAGSLGVSNSEKMNNATLGSFPRSTDPPTNNSTSVPSTEGSHDRRKVISVEAVSNPRRFVPQTVTRDIISRVITRMPTPAEKWKDYPSEMKDELFKEFTGKYKFTSDYDCNMSRTVWERTCMDRYPDHLKNARKMALREVNSVNLVDTKGHAPKGMKAEVWDGLVDIWLKPEWKKKSDANRCNRAALPDAVLHTGGSINFGEHKIRMEKELNHEVSYRDVYDRVHKKKDGEYVSQRSKKFIETYDTAMLEKYGDDFSTHPAVDSNLWADTFSHHKTRQTFVGRSLDLGGFVSASSAEFTGPSNILATSEEAIKKAVDKAMSSFVQSQLMPLLEPIISLIQSMHKAPMQGEFAEGHMDAR*