AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1071530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 62 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACAGTGATAATTTGTATTA+TGG 0.130227 7_2:+68051687 None:intergenic
GGTTCCTCCTTCAAGAATTC+TGG 0.172529 7_2:-68051258 Msa1071530:three_prime_UTR
TTCCCTCATGTAGTATTTAA+AGG 0.173459 7_2:+68050957 None:intergenic
TTACAATGATAATGTGTATT+TGG 0.176865 7_2:+68053434 None:intergenic
ATTAAACTTGGAAATGAATT+AGG 0.210390 7_2:-68054234 Msa1071530:intron
TAATTCATTTCCAAGTTTAA+TGG 0.234342 7_2:+68054236 None:intergenic
CAGAATTTGCAGTGCAGCGC+AGG 0.294148 7_2:+68050866 None:intergenic
TAATTATAAATATAATTGTT+AGG 0.307154 7_2:-68051371 Msa1071530:three_prime_UTR
AGATAAGATTATAAAAGTTC+AGG 0.343347 7_2:-68054320 Msa1071530:five_prime_UTR
ACAGTGATAATTTGTATTAT+GGG 0.354259 7_2:+68051688 None:intergenic
GACTCTGCATAAGACAATAA+TGG 0.355828 7_2:-68051404 Msa1071530:three_prime_UTR
AGACAATTCCGCAAAGCGTT+TGG 0.379036 7_2:+68051774 None:intergenic
CAAAATGATGCCATTAAACT+TGG 0.402093 7_2:-68054246 Msa1071530:five_prime_UTR
TAGTACAAAACAGAGGTATA+TGG 0.414342 7_2:+68051448 None:intergenic
CGAAAGAGCCTTGGAGAGAA+AGG 0.423381 7_2:-68050933 Msa1071530:three_prime_UTR
AGAAAGAGCCTTGGAGAGAA+AGG 0.426750 7_2:-68050890 Msa1071530:three_prime_UTR
ACTCTGCATAAGACAATAAT+GGG 0.428670 7_2:-68051403 Msa1071530:three_prime_UTR
GCCAGATTGTATGTCAGATA+TGG 0.437535 7_2:+68054477 None:intergenic
GCCTTCATACTGCTTTCTGC+TGG 0.438656 7_2:-68051284 Msa1071530:three_prime_UTR
AATTATAAATATAATTGTTA+GGG 0.440124 7_2:-68051370 Msa1071530:three_prime_UTR
GTTCCTCCTTCAAGAATTCT+GGG 0.442364 7_2:-68051257 Msa1071530:three_prime_UTR
AAATACAGCTATAGCAGAAA+AGG 0.446789 7_2:+68054509 None:intergenic
TTGTCTCCCAGAATTCTTGA+AGG 0.447191 7_2:+68051251 None:intergenic
GCTGCTATCCAAACGCTTTG+CGG 0.447265 7_2:-68051782 Msa1071530:CDS
CTCAAGCTTGTTGCATATGT+TGG 0.452344 7_2:-68052499 Msa1071530:CDS
CAACTAAGAGCTTATCTCAT+TGG 0.455962 7_2:+68051134 None:intergenic
CAGGAGAGCACGTGTCTTTG+TGG 0.456160 7_2:-68054301 Msa1071530:five_prime_UTR
TTGGAGAGAAAGGAAGGGCT+TGG 0.464845 7_2:-68050923 Msa1071530:three_prime_UTR
GGCTACTCTTCCTTCCTGCC+AGG 0.465761 7_2:+68054271 None:intergenic
AAAGAGCTACCGTGTAGGTC+AGG 0.490343 7_2:+68054556 None:intergenic
AAGCCCTTCCTTTCTCTCCA+AGG 0.495149 7_2:+68050925 None:intergenic
TTTGCGGAATTGTCTGATGA+AGG 0.504707 7_2:-68051766 Msa1071530:CDS
CTTCATCTCATAGTTTCTCA+AGG 0.506698 7_2:+68053509 None:intergenic
TGAGGGAAGCGAAAGAGCCT+TGG 0.511561 7_2:-68050942 Msa1071530:three_prime_UTR
GCCATATCTGACATACAATC+TGG 0.513874 7_2:-68054478 Msa1071530:five_prime_UTR
CGTTTGGATAGCAGCCAAGC+AGG 0.516208 7_2:+68051790 None:intergenic
CTTGAGAAACTATGAGATGA+AGG 0.518610 7_2:-68053508 Msa1071530:exon
GCGCAGGGCCTTTCTCTCCA+AGG 0.526608 7_2:+68050882 None:intergenic
TGTCTTTGTGGAAGTAGTCC+TGG 0.529821 7_2:-68054289 Msa1071530:five_prime_UTR
TTGCCTTTAAATACTACATG+AGG 0.534140 7_2:-68050960 Msa1071530:three_prime_UTR
GCGTGAGAGAGAAAGAGCCT+TGG 0.539498 7_2:-68050899 Msa1071530:three_prime_UTR
GCAGAAAAGGCCTTAAAATG+TGG 0.539910 7_2:+68054522 None:intergenic
AGAGCCTTGGAGAGAAAGGA+AGG 0.541262 7_2:-68050929 Msa1071530:three_prime_UTR
GCCAGCAGAAAGCAGTATGA+AGG 0.568670 7_2:+68051283 None:intergenic
AGAATTTGCAGTGCAGCGCA+GGG 0.576307 7_2:+68050867 None:intergenic
CAGAAAAGGCCTTAAAATGT+GGG 0.578792 7_2:+68054523 None:intergenic
CATACTGCTTTCTGCTGGCG+TGG 0.581915 7_2:-68051279 Msa1071530:three_prime_UTR
TTTGTGGAAGTAGTCCTGGC+AGG 0.584999 7_2:-68054285 Msa1071530:five_prime_UTR
CAAAATGTGATGGCAAGGCA+AGG 0.585482 7_2:-68050986 Msa1071530:three_prime_UTR
AGAGGCATAAAGTTGCATGA+TGG 0.590712 7_2:-68051225 Msa1071530:three_prime_UTR
TCTCCCAGAATTCTTGAAGG+AGG 0.595273 7_2:+68051254 None:intergenic
TGGAAGTAGTCCTGGCAGGA+AGG 0.600387 7_2:-68054281 Msa1071530:five_prime_UTR
CTTTATATTCCTGACCTACA+CGG 0.609660 7_2:-68054565 Msa1071530:five_prime_UTR
TGCCTTTAAATACTACATGA+GGG 0.610711 7_2:-68050959 Msa1071530:three_prime_UTR
GCTTGCAAAATGTGATGGCA+AGG 0.615832 7_2:-68050991 Msa1071530:three_prime_UTR
ATCACATCTGCAGATTTGTG+TGG 0.619954 7_2:+68051741 None:intergenic
ACAAATCTGCAGATGTGATG+AGG 0.630753 7_2:-68051737 Msa1071530:CDS
GAGCCTTGGAGAGAAAGGAA+GGG 0.646139 7_2:-68050928 Msa1071530:three_prime_UTR
AGCTGTCAAAGCTACTGATG+AGG 0.663227 7_2:-68053485 Msa1071530:CDS
AAAACAAAGAGCTACCGTGT+AGG 0.675010 7_2:+68054551 None:intergenic
AATTCTGGGAGACAAAGAAG+AGG 0.676675 7_2:-68051243 Msa1071530:three_prime_UTR
ACATTCTTAGTACAAAACAG+AGG 0.764301 7_2:+68051441 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATTTAAATTATTTAAT+TGG - chr7_2:68053128-68053147 Msa1071530:intron 0.0%
!!! ATTAATTTTATTAATTTTAT+TGG - chr7_2:68053495-68053514 Msa1071530:CDS 0.0%
!!! ATTGGTAAAATTATAATTTT+TGG - chr7_2:68052320-68052339 Msa1071530:intron 10.0%
!! GAAATAAAATGAAAAAAATT+TGG + chr7_2:68052488-68052507 None:intergenic 10.0%
!! AAACATAGTTTGAAAAAAAA+GGG + chr7_2:68050969-68050988 None:intergenic 15.0%
!!! TTTTTTTTATCTGTTTTTTG+TGG - chr7_2:68051241-68051260 Msa1071530:three_prime_UTR 15.0%
!! TATTTCAATTGTTAAATGAA+AGG + chr7_2:68051753-68051772 None:intergenic 15.0%
!! TGAAGCAAAAATTAATAAAA+TGG - chr7_2:68051806-68051825 Msa1071530:CDS 15.0%
!!! ATTTAATTTAAATATGTGTG+TGG + chr7_2:68053114-68053133 None:intergenic 15.0%
!! ATTGGTAAAATTATAATTTC+TGG - chr7_2:68053536-68053555 Msa1071530:intron 15.0%
!!! TATTATTTTTAACTTGCAAT+TGG - chr7_2:68053866-68053885 Msa1071530:intron 15.0%
!! CAAACATAGTTTGAAAAAAA+AGG + chr7_2:68050970-68050989 None:intergenic 20.0%
!!! TTTGTAACATTTTTTAGCTA+TGG + chr7_2:68050996-68051015 None:intergenic 20.0%
!! AGATAAGATTATAAAAGTTC+AGG - chr7_2:68051101-68051120 Msa1071530:three_prime_UTR 20.0%
!! TAATTCATTTCCAAGTTTAA+TGG + chr7_2:68051188-68051207 None:intergenic 20.0%
!! ATTAAACTTGGAAATGAATT+AGG - chr7_2:68051187-68051206 Msa1071530:three_prime_UTR 20.0%
!!! TTACAATGATAATGTGTATT+TGG + chr7_2:68051990-68052009 None:intergenic 20.0%
!!! TATTATTTTTAGCTTGCAAT+TGG - chr7_2:68052157-68052176 Msa1071530:intron 20.0%
!!! AAAAATGGGTAATGTTTTTT+CGG - chr7_2:68052365-68052384 Msa1071530:intron 20.0%
!! ATTCAATTGATGAAATGAAA+AGG + chr7_2:68052646-68052665 None:intergenic 20.0%
!! AGAAGTTTGAACAAATAAAT+CGG + chr7_2:68053084-68053103 None:intergenic 20.0%
!! AACATGATTAACCATTATAA+TGG - chr7_2:68053262-68053281 Msa1071530:intron 20.0%
!! TTATATGTAAGGAAAATATG+AGG + chr7_2:68053379-68053398 None:intergenic 20.0%
!! TCATTCAATCATTATATGTA+AGG + chr7_2:68053390-68053409 None:intergenic 20.0%
!!! ACAGTGATAATTTGTATTAT+GGG + chr7_2:68053736-68053755 None:intergenic 20.0%
!!! TACAGTGATAATTTGTATTA+TGG + chr7_2:68053737-68053756 None:intergenic 20.0%
!!! TTTTTTCTTCCCACATTTTA+AGG - chr7_2:68050889-68050908 Msa1071530:three_prime_UTR 25.0%
!! CAAAACTCAAAGTATTAACA+AGG + chr7_2:68051432-68051451 None:intergenic 25.0%
!! AGAATTTGAGTTAAAGTCAA+AGG + chr7_2:68051521-68051540 None:intergenic 25.0%
! TTTGTTACACAAAGTTTCAT+GGG + chr7_2:68051564-68051583 None:intergenic 25.0%
!! AAGTTTTCAAAAGATGGTTA+TGG - chr7_2:68051620-68051639 Msa1071530:three_prime_UTR 25.0%
!! ATAAGATATTAGCTTCAACA+AGG + chr7_2:68051849-68051868 None:intergenic 25.0%
! TTGTTGAAGCTAATATCTTA+TGG - chr7_2:68051848-68051867 Msa1071530:intron 25.0%
! TACTAGGAGAGTTTATTATA+AGG + chr7_2:68052223-68052242 None:intergenic 25.0%
!!! TAATTTTTGGAGACATCTTA+TGG - chr7_2:68052333-68052352 Msa1071530:intron 25.0%
!!! TTATGGATTAGACAGAAAAA+TGG - chr7_2:68052350-68052369 Msa1071530:intron 25.0%
!!! TATGGATTAGACAGAAAAAT+GGG - chr7_2:68052351-68052370 Msa1071530:intron 25.0%
!! AAAAATTTGGATACCCATTT+TGG + chr7_2:68052475-68052494 None:intergenic 25.0%
! TATTCATCGTTATCAAAAGA+AGG + chr7_2:68052529-68052548 None:intergenic 25.0%
!!! TTAATACATTGTTTTGCCTT+TGG - chr7_2:68052779-68052798 Msa1071530:intron 25.0%
! AGAAAATAAACTCAAAGTGA+AGG + chr7_2:68052834-68052853 None:intergenic 25.0%
!!! AAGTTTTCAAATGTTGGTTA+TGG - chr7_2:68053207-68053226 Msa1071530:intron 25.0%
!! TACTATGAGAGTTTTCTATA+AGG + chr7_2:68053317-68053336 None:intergenic 25.0%
! CAATTGTAGAGTATGAAAAA+TGG - chr7_2:68054339-68054358 Msa1071530:five_prime_UTR 25.0%
!!! GTAAAAAGAAGCATAGATTT+TGG - chr7_2:68054408-68054427 Msa1071530:intron 25.0%
AAATACAGCTATAGCAGAAA+AGG + chr7_2:68050915-68050934 None:intergenic 30.0%
AGACGATTTACAAATAGTCT+TGG + chr7_2:68051025-68051044 None:intergenic 30.0%
! ATCGTCTTTTGTGAAACTTT+TGG - chr7_2:68051038-68051057 Msa1071530:three_prime_UTR 30.0%
! GTTTAATGGCATCATTTTGA+TGG + chr7_2:68051174-68051193 None:intergenic 30.0%
CAAAATGATGCCATTAAACT+TGG - chr7_2:68051175-68051194 Msa1071530:three_prime_UTR 30.0%
!!! TTATCTGTTTTTTGTGGAAG+AGG - chr7_2:68051247-68051266 Msa1071530:three_prime_UTR 30.0%
TTCAGAAGCAATGTTGTAAA+GGG + chr7_2:68051484-68051503 None:intergenic 30.0%
ATTCAGAAGCAATGTTGTAA+AGG + chr7_2:68051485-68051504 None:intergenic 30.0%
! GTTTGTTACACAAAGTTTCA+TGG + chr7_2:68051565-68051584 None:intergenic 30.0%
!! CCATCTTTTGAAAACTTTTG+TGG + chr7_2:68051617-68051636 None:intergenic 30.0%
! CCACAAAAGTTTTCAAAAGA+TGG - chr7_2:68051614-68051633 Msa1071530:three_prime_UTR 30.0%
TTTAATCTACACAAATCTGC+AGG - chr7_2:68051884-68051903 Msa1071530:intron 30.0%
AATGAGAAAGAACACAAAAG+TGG + chr7_2:68052088-68052107 None:intergenic 30.0%
!!! TTTTAGCTTGCAATTGGTTA+TGG - chr7_2:68052163-68052182 Msa1071530:intron 30.0%
TCTTACAATAGACACATGTT+TGG - chr7_2:68052293-68052312 Msa1071530:intron 30.0%
! TAACGATGAATACCTTTTTC+CGG - chr7_2:68052537-68052556 Msa1071530:intron 30.0%
CAGTTATGAAAAAGGAATAC+CGG + chr7_2:68052559-68052578 None:intergenic 30.0%
CACAAATTCCACATAATCAA+GGG + chr7_2:68052703-68052722 None:intergenic 30.0%
TAAAAGCAATACAAAGCCAA+AGG + chr7_2:68052798-68052817 None:intergenic 30.0%
AGTGTTGTAAAGGGAAAATT+TGG + chr7_2:68053016-68053035 None:intergenic 30.0%
TTTGGAAGTAGTGTTGTAAA+GGG + chr7_2:68053025-68053044 None:intergenic 30.0%
ATTTGGAAGTAGTGTTGTAA+AGG + chr7_2:68053026-68053045 None:intergenic 30.0%
TACAACACTACTTCCAAATT+TGG - chr7_2:68053027-68053046 Msa1071530:intron 30.0%
AAGGAAACAACAACCAAATT+TGG + chr7_2:68053043-68053062 None:intergenic 30.0%
!! TAAATCGGATTTTGAGTCAT+AGG + chr7_2:68053069-68053088 None:intergenic 30.0%
! GAAAACTTTTGAGCATATGT+TGG + chr7_2:68053195-68053214 None:intergenic 30.0%
! GCTCAAAAGTTTTCAAATGT+TGG - chr7_2:68053201-68053220 Msa1071530:intron 30.0%
AGGTATGGCAAAAGAAAAAT+TGG + chr7_2:68053297-68053316 None:intergenic 30.0%
! TGAGAGTTTTCTATAAGGTA+TGG + chr7_2:68053312-68053331 None:intergenic 30.0%
GAAAATATGAGGAATCCAAA+AGG + chr7_2:68053368-68053387 None:intergenic 30.0%
AGACACATGTTTAGTAACAT+TGG - chr7_2:68053518-68053537 Msa1071530:five_prime_UTR 30.0%
!! TTGATTGTTTTGTGCAGAAT+AGG - chr7_2:68053892-68053911 Msa1071530:intron 30.0%
!! TGATTGTTTTGTGCAGAATA+GGG - chr7_2:68053893-68053912 Msa1071530:intron 30.0%
! TAGTACAAAACAGAGGTATA+TGG + chr7_2:68053976-68053995 None:intergenic 30.0%
ACATTCTTAGTACAAAACAG+AGG + chr7_2:68053983-68054002 None:intergenic 30.0%
ACTCTGCATAAGACAATAAT+GGG - chr7_2:68054018-68054037 Msa1071530:intron 30.0%
TTGCCTTTAAATACTACATG+AGG - chr7_2:68054461-68054480 Msa1071530:intron 30.0%
TGCCTTTAAATACTACATGA+GGG - chr7_2:68054462-68054481 Msa1071530:intron 30.0%
TTCCCTCATGTAGTATTTAA+AGG + chr7_2:68054467-68054486 None:intergenic 30.0%
CTTTATATTCCTGACCTACA+CGG - chr7_2:68050856-68050875 Msa1071530:three_prime_UTR 35.0%
CAGAAAAGGCCTTAAAATGT+GGG + chr7_2:68050901-68050920 None:intergenic 35.0%
AATTCGTTGCAAACTTCAGT+TGG - chr7_2:68051312-68051331 Msa1071530:three_prime_UTR 35.0%
ATTCGTTGCAAACTTCAGTT+GGG - chr7_2:68051313-68051332 Msa1071530:three_prime_UTR 35.0%
TACAACATTGCTTCTGAATC+TGG - chr7_2:68051486-68051505 Msa1071530:three_prime_UTR 35.0%
GACTAAGAGAGTCGATTAAA+AGG + chr7_2:68051680-68051699 None:intergenic 35.0%
CTTCATCTCATAGTTTCTCA+AGG + chr7_2:68051915-68051934 None:intergenic 35.0%
CTTGAGAAACTATGAGATGA+AGG - chr7_2:68051913-68051932 Msa1071530:intron 35.0%
AGAAAGAACACAAAAGTGGA+AGG + chr7_2:68052084-68052103 None:intergenic 35.0%
! TTCACGTTCACACTTTTACT+AGG + chr7_2:68052239-68052258 None:intergenic 35.0%
AAAAATATGCAGAGTCCAAG+AGG + chr7_2:68052274-68052293 None:intergenic 35.0%
! AGACACATGTTTGGTAACAT+TGG - chr7_2:68052302-68052321 Msa1071530:intron 35.0%
!!! ATTTTGGTTCGATTCTTGCA+TGG + chr7_2:68052459-68052478 None:intergenic 35.0%
ATGCAAGAATCGAACCAAAA+TGG - chr7_2:68052458-68052477 Msa1071530:CDS 35.0%
TGCAAGAATCGAACCAAAAT+GGG - chr7_2:68052459-68052478 Msa1071530:CDS 35.0%
GAAAAAGGAATACCGGAAAA+AGG + chr7_2:68052552-68052571 None:intergenic 35.0%
CTATGCAGCAGTTATGAAAA+AGG + chr7_2:68052567-68052586 None:intergenic 35.0%
CTTAATCATTATGATGCCCT+TGG + chr7_2:68052611-68052630 None:intergenic 35.0%
ACGTTATTCCCTTGATTATG+TGG - chr7_2:68052692-68052711 Msa1071530:intron 35.0%
CCACAAATTCCACATAATCA+AGG + chr7_2:68052704-68052723 None:intergenic 35.0%
CCTTGATTATGTGGAATTTG+TGG - chr7_2:68052701-68052720 Msa1071530:intron 35.0%
! TTTCTTTTGATCAGGTGGTT+AGG - chr7_2:68052898-68052917 Msa1071530:intron 35.0%
TGTTAGTGTTGCAAAGTTAG+AGG - chr7_2:68052988-68053007 Msa1071530:intron 35.0%
!! GATTTTGAGTCATAGGTCAA+AGG + chr7_2:68053062-68053081 None:intergenic 35.0%
! TAATTTCTGGAGACGTCTTA+TGG - chr7_2:68053549-68053568 Msa1071530:intron 35.0%
GACTCTGCATAAGACAATAA+TGG - chr7_2:68054017-68054036 Msa1071530:intron 35.0%
CAACTAAGAGCTTATCTCAT+TGG + chr7_2:68054290-68054309 None:intergenic 35.0%
!!! TTTTGGCTTGCAAAATGTGA+TGG - chr7_2:68054425-68054444 Msa1071530:intron 35.0%
AAAACAAAGAGCTACCGTGT+AGG + chr7_2:68050873-68050892 None:intergenic 40.0%
GCAGAAAAGGCCTTAAAATG+TGG + chr7_2:68050902-68050921 None:intergenic 40.0%
GCCATATCTGACATACAATC+TGG - chr7_2:68050943-68050962 Msa1071530:three_prime_UTR 40.0%
GCCAGATTGTATGTCAGATA+TGG + chr7_2:68050947-68050966 None:intergenic 40.0%
! GAAACTTTTGGCATGTTTGC+AGG - chr7_2:68051050-68051069 Msa1071530:three_prime_UTR 40.0%
!!! TCTGTTTTTTGTGGAAGAGG+TGG - chr7_2:68051250-68051269 Msa1071530:three_prime_UTR 40.0%
! TTTTGTGGAAGAGGTGGAAA+TGG - chr7_2:68051256-68051275 Msa1071530:three_prime_UTR 40.0%
TTCGTTGCAAACTTCAGTTG+GGG - chr7_2:68051314-68051333 Msa1071530:three_prime_UTR 40.0%
! TTGATGCATCGTTTTGCCTT+TGG - chr7_2:68051368-68051387 Msa1071530:three_prime_UTR 40.0%
GAGCAGAAACACAAATCCAA+AGG + chr7_2:68051387-68051406 None:intergenic 40.0%
!! AAGGGATAATTTGCTACCTC+TGG + chr7_2:68051466-68051485 None:intergenic 40.0%
AGAGAGTCGATTAAAAGGCA+TGG + chr7_2:68051675-68051694 None:intergenic 40.0%
GAATTCCATTGCATGCTTCT+AGG - chr7_2:68051713-68051732 Msa1071530:CDS 40.0%
GAATTCCATTGTATGCCTCT+TGG - chr7_2:68052256-68052275 Msa1071530:intron 40.0%
! CTGCTGCATAGATCTTTTTG+TGG - chr7_2:68052576-68052595 Msa1071530:intron 40.0%
! TTTTGTGGCAGCTAAATCCA+AGG - chr7_2:68052591-68052610 Msa1071530:intron 40.0%
TTTGTGGCAGCTAAATCCAA+GGG - chr7_2:68052592-68052611 Msa1071530:intron 40.0%
TCTCGCAACAGTATAAGAGA+CGG - chr7_2:68052871-68052890 Msa1071530:intron 40.0%
!! CTCAAGCTTGTTGCATATGT+TGG - chr7_2:68052922-68052941 Msa1071530:intron 40.0%
GGCTTGCAAGTCCATTATAA+TGG + chr7_2:68053276-68053295 None:intergenic 40.0%
! GAATTCCATTGTACGCCTTT+TGG - chr7_2:68053350-68053369 Msa1071530:intron 40.0%
! TTTTTGTCGACATACCTGCT+TGG - chr7_2:68053617-68053636 Msa1071530:intron 40.0%
TTTGCGGAATTGTCTGATGA+AGG - chr7_2:68053655-68053674 Msa1071530:intron 40.0%
ATCACATCTGCAGATTTGTG+TGG + chr7_2:68053683-68053702 None:intergenic 40.0%
ACAAATCTGCAGATGTGATG+AGG - chr7_2:68053684-68053703 Msa1071530:intron 40.0%
GTTCCTCCTTCAAGAATTCT+GGG - chr7_2:68054164-68054183 Msa1071530:intron 40.0%
TTGTCTCCCAGAATTCTTGA+AGG + chr7_2:68054173-68054192 None:intergenic 40.0%
! AATTCTGGGAGACAAAGAAG+AGG - chr7_2:68054178-68054197 Msa1071530:intron 40.0%
! AGAGGCATAAAGTTGCATGA+TGG - chr7_2:68054196-68054215 Msa1071530:intron 40.0%
TGTCTTTGTGGAAGTAGTCC+TGG - chr7_2:68051132-68051151 Msa1071530:three_prime_UTR 45.0%
TCGTTGCAAACTTCAGTTGG+GGG - chr7_2:68051315-68051334 Msa1071530:three_prime_UTR 45.0%
! TTTTGCTAGTGCAAAGCCAG+AGG - chr7_2:68051447-68051466 Msa1071530:three_prime_UTR 45.0%
! CAAGTCCTAGAAGCATGCAA+TGG + chr7_2:68051721-68051740 None:intergenic 45.0%
AGCTGTCAAAGCTACTGATG+AGG - chr7_2:68051936-68051955 Msa1071530:intron 45.0%
! AGAGTCCAAGAGGCATACAA+TGG + chr7_2:68052264-68052283 None:intergenic 45.0%
!!! ACGGCTGGTTTCTTTTGATC+AGG - chr7_2:68052890-68052909 Msa1071530:intron 45.0%
!! GCTGGTTTCTTTTGATCAGG+TGG - chr7_2:68052893-68052912 Msa1071530:intron 45.0%
! GGAATCCAAAAGGCGTACAA+TGG + chr7_2:68053358-68053377 None:intergenic 45.0%
! AGACAATTCCGCAAAGCGTT+TGG + chr7_2:68053650-68053669 None:intergenic 45.0%
GGTTCCTCCTTCAAGAATTC+TGG - chr7_2:68054163-68054182 Msa1071530:intron 45.0%
TCTCCCAGAATTCTTGAAGG+AGG + chr7_2:68054170-68054189 None:intergenic 45.0%
GCTTGCAAAATGTGATGGCA+AGG - chr7_2:68054430-68054449 Msa1071530:intron 45.0%
CAAAATGTGATGGCAAGGCA+AGG - chr7_2:68054435-68054454 Msa1071530:intron 45.0%
AGAAAGAGCCTTGGAGAGAA+AGG - chr7_2:68054531-68054550 Msa1071530:five_prime_UTR 45.0%
!!! AAGATTTTTTTTTTTAAAAA+GGG + chr7_2:68051347-68051366 None:intergenic 5.0%
!!! TAAGATTTTTTTTTTTAAAA+AGG + chr7_2:68051348-68051367 None:intergenic 5.0%
!! AATAATAACAATAATAATAT+AGG - chr7_2:68053418-68053437 Msa1071530:intron 5.0%
!! TAATTATAAATATAATTGTT+AGG - chr7_2:68054050-68054069 Msa1071530:intron 5.0%
!! AATTATAAATATAATTGTTA+GGG - chr7_2:68054051-68054070 Msa1071530:intron 5.0%
AAAGAGCTACCGTGTAGGTC+AGG + chr7_2:68050868-68050887 None:intergenic 50.0%
TTTGTGGAAGTAGTCCTGGC+AGG - chr7_2:68051136-68051155 Msa1071530:three_prime_UTR 50.0%
CGTTGCAAACTTCAGTTGGG+GGG - chr7_2:68051316-68051335 Msa1071530:three_prime_UTR 50.0%
GCAACAGTATAAGAGACGGC+TGG - chr7_2:68052875-68052894 Msa1071530:intron 50.0%
GCTGCTATCCAAACGCTTTG+CGG - chr7_2:68053639-68053658 Msa1071530:intron 50.0%
GCCTTCATACTGCTTTCTGC+TGG - chr7_2:68054137-68054156 Msa1071530:intron 50.0%
GCCAGCAGAAAGCAGTATGA+AGG + chr7_2:68054141-68054160 None:intergenic 50.0%
CGAAAGAGCCTTGGAGAGAA+AGG - chr7_2:68054488-68054507 Msa1071530:five_prime_UTR 50.0%
AGAGCCTTGGAGAGAAAGGA+AGG - chr7_2:68054492-68054511 Msa1071530:five_prime_UTR 50.0%
GAGCCTTGGAGAGAAAGGAA+GGG - chr7_2:68054493-68054512 Msa1071530:five_prime_UTR 50.0%
AAGCCCTTCCTTTCTCTCCA+AGG + chr7_2:68054499-68054518 None:intergenic 50.0%
TTGGAGAGAAAGGAAGGGCT+TGG - chr7_2:68054498-68054517 Msa1071530:five_prime_UTR 50.0%
AGAATTTGCAGTGCAGCGCA+GGG + chr7_2:68054557-68054576 None:intergenic 50.0%
! CAGGAGAGCACGTGTCTTTG+TGG - chr7_2:68051120-68051139 Msa1071530:three_prime_UTR 55.0%
TGGAAGTAGTCCTGGCAGGA+AGG - chr7_2:68051140-68051159 Msa1071530:three_prime_UTR 55.0%
CGTTTGGATAGCAGCCAAGC+AGG + chr7_2:68053634-68053653 None:intergenic 55.0%
CATACTGCTTTCTGCTGGCG+TGG - chr7_2:68054142-68054161 Msa1071530:intron 55.0%
TGAGGGAAGCGAAAGAGCCT+TGG - chr7_2:68054479-68054498 Msa1071530:five_prime_UTR 55.0%
GCGTGAGAGAGAAAGAGCCT+TGG - chr7_2:68054522-68054541 Msa1071530:five_prime_UTR 55.0%
CAGAATTTGCAGTGCAGCGC+AGG + chr7_2:68054558-68054577 None:intergenic 55.0%
GGCTACTCTTCCTTCCTGCC+AGG + chr7_2:68051153-68051172 None:intergenic 60.0%
GCGCAGGGCCTTTCTCTCCA+AGG + chr7_2:68054542-68054561 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 68050854 68054589 68050854 ID=Msa1071530;Name=Msa1071530
chr7_2 mRNA 68050854 68054589 68050854 ID=Msa1071530-mRNA-1;Parent=Msa1071530;Name=Msa1071530-mRNA-1;_AED=0.04;_eAED=0.04;_QI=287|0.75|0.8|1|0|0|5|806|108
chr7_2 exon 68050854 68051832 68050854 ID=Msa1071530-mRNA-1:exon:14833;Parent=Msa1071530-mRNA-1
chr7_2 exon 68052455 68052531 68052455 ID=Msa1071530-mRNA-1:exon:14832;Parent=Msa1071530-mRNA-1
chr7_2 exon 68053438 68053537 68053438 ID=Msa1071530-mRNA-1:exon:14831;Parent=Msa1071530-mRNA-1
chr7_2 exon 68054235 68054371 68054235 ID=Msa1071530-mRNA-1:exon:14830;Parent=Msa1071530-mRNA-1
chr7_2 exon 68054463 68054589 68054463 ID=Msa1071530-mRNA-1:exon:14829;Parent=Msa1071530-mRNA-1
chr7_2 five_prime_UTR 68054463 68054589 68054463 ID=Msa1071530-mRNA-1:five_prime_utr;Parent=Msa1071530-mRNA-1
chr7_2 five_prime_UTR 68054235 68054371 68054235 ID=Msa1071530-mRNA-1:five_prime_utr;Parent=Msa1071530-mRNA-1
chr7_2 five_prime_UTR 68053515 68053537 68053515 ID=Msa1071530-mRNA-1:five_prime_utr;Parent=Msa1071530-mRNA-1
chr7_2 CDS 68053438 68053514 68053438 ID=Msa1071530-mRNA-1:cds;Parent=Msa1071530-mRNA-1
chr7_2 CDS 68052455 68052531 68052455 ID=Msa1071530-mRNA-1:cds;Parent=Msa1071530-mRNA-1
chr7_2 CDS 68051660 68051832 68051660 ID=Msa1071530-mRNA-1:cds;Parent=Msa1071530-mRNA-1
chr7_2 three_prime_UTR 68050854 68051659 68050854 ID=Msa1071530-mRNA-1:three_prime_utr;Parent=Msa1071530-mRNA-1
Gene Sequence

>Msa1071530

ATGAAGGAGCTGTCAAAGCTACTGATGAGGACTTGTTTACATGCTTGCTTGCTGCTGTCCAAATACACATTATCATTGTGGTTAGGACTCAAGCTTGTTGCATATGTTGGTATAGTTGATGCTTCAAATGAAGAAAAGCACAATTTTGAGCTTTATCATATTTTTGTCGACATACCTGCTTGGCTGCTATCCAAACGCTTTGCGGAATTGTCTGATGAAGGATCCACACAAATCTGCAGATGTGATGAGGACTTGTTACATGCTTGTTTGTTGCTGCCCATAATACAAATTATCACTGTAAGTAAAAAAATGAAAACAATTGTGTAG
Protein sequence

>Msa1071530

MKELSKLLMRTCLHACLLLSKYTLSLWLGLKLVAYVGIVDASNEEKHNFELYHIFVDIPAWLLSKRFAELSDEGSTQICRCDEDLLHACLLLPIIQIITVSKKMKTIV*