AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene002256

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002256.t1 MTR_2g009800 98.919 555 6 0 1 555 1 555 0.0 1131
MS.gene002256.t1 MTR_2g101620 91.712 555 46 0 1 555 1 555 0.0 1055
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002256.t1 AT5G14050 62.655 565 182 11 1 555 1 546 0.0 678

Find 115 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTATTCACCTCCTGAATT+TGG 0.168421 2.3:-73233739 MS.gene002256:CDS
GACTGGGCGCAGCTGGATTC+AGG 0.199184 2.3:-73233403 MS.gene002256:CDS
ATGGAACTGAACTGAATTAA+TGG 0.221155 2.3:+73233180 None:intergenic
GAGGATGAGGATTTGATATC+TGG 0.277710 2.3:-73233481 MS.gene002256:CDS
TAGCCAAAGTCAACAGATTA+AGG 0.291410 2.3:-73233519 MS.gene002256:CDS
CTGAGAAGTCAGCATCTTCT+TGG 0.323321 2.3:+73233620 None:intergenic
AAAGCTATCATTTGAGAATC+AGG 0.336697 2.3:+73232890 None:intergenic
AAGGAAGATACTTTCAATCT+TGG 0.340340 2.3:+73233081 None:intergenic
GAAGAGTATTGAAGTGTTTG+AGG 0.342182 2.3:-73232918 MS.gene002256:CDS
AGATTATCAATGGTCTTGAT+AGG 0.358024 2.3:+73232551 None:intergenic
TACTTCAGGCATGGTGATAA+TGG 0.362722 2.3:+73232294 None:intergenic
GGTCAGCTGCTTCTTGCTGC+TGG 0.365476 2.3:-73233151 MS.gene002256:CDS
TCAACAGATTAAGGAAGTTA+AGG 0.371537 2.3:-73233510 MS.gene002256:CDS
AGGAACTGACTGGGCGCAGC+TGG 0.391668 2.3:-73233410 MS.gene002256:CDS
GTTAAGGAAGGAGGAGGATG+AGG 0.392451 2.3:-73233494 MS.gene002256:CDS
TGGGCTAAAATCAATACAAC+GGG 0.399262 2.3:+73232376 None:intergenic
TGGAACTGAACTGAATTAAT+GGG 0.402013 2.3:+73233181 None:intergenic
CTTCCTTAATCTGTTGACTT+TGG 0.407960 2.3:+73233516 None:intergenic
GGGTCTCAGGTGATAATATC+AGG 0.411556 2.3:-73233019 MS.gene002256:CDS
TTTCTTGCCCGATGGGTCTC+AGG 0.414581 2.3:-73233032 MS.gene002256:CDS
ATGGCTGTAGGAAATGCTTC+AGG 0.420095 2.3:-73232341 MS.gene002256:CDS
TTATAGTCTTGATTTAGTTA+AGG 0.420893 2.3:-73232981 MS.gene002256:CDS
TTCGAATGGGGCAATCTTCA+AGG 0.424695 2.3:+73233062 None:intergenic
TCAGTTCAGTTCCATCGAAA+TGG 0.438541 2.3:-73233172 MS.gene002256:CDS
AAATACTAGCAATCTGTTCA+AGG 0.442805 2.3:-73232487 MS.gene002256:CDS
TTCAGTATTCAGATTATCAA+TGG 0.445525 2.3:+73232541 None:intergenic
AATATACAAGATCCCGCTAA+TGG 0.453091 2.3:-73233205 MS.gene002256:CDS
TACAATAGCGAGGAATTTCT+CGG 0.460074 2.3:-73232587 MS.gene002256:CDS
GAGCCGAGGTTACGAGGATC+TGG 0.461776 2.3:-73233320 MS.gene002256:CDS
GTAGATGAAGGCTGTTTAAA+CGG 0.466326 2.3:-73232680 MS.gene002256:CDS
TCACCTCCTGAATTTGGAAA+GGG 0.467907 2.3:-73233733 MS.gene002256:CDS
GCTGCAAAATGTGTCCCTAC+TGG 0.467974 2.3:+73232638 None:intergenic
AAGGCTAGAGTTGATAAGAT+TGG 0.470242 2.3:-73232962 MS.gene002256:CDS
AGCTCAACCGAGGAACTGAC+TGG 0.472580 2.3:-73233420 MS.gene002256:CDS
TGATGGTCATATTTACCATT+GGG 0.474225 2.3:-73232735 MS.gene002256:CDS
GATAAGATTGGTCCGCTGTT+GGG 0.475592 2.3:-73232950 MS.gene002256:CDS
ACTGGGCGCAGCTGGATTCA+GGG 0.478998 2.3:-73233402 MS.gene002256:CDS
AAGCATCTTTCTTGCCCGAT+GGG 0.479466 2.3:-73233039 MS.gene002256:CDS
TTTGCAGCTGGTTCAGCTAG+TGG 0.484592 2.3:-73232623 MS.gene002256:CDS
GTGATGGTCATATTTACCAT+TGG 0.489547 2.3:-73232736 MS.gene002256:CDS
AAGGAAGTTAAGGAAGGAGG+AGG 0.489554 2.3:-73233500 MS.gene002256:CDS
AAAGCCGGTGTGGGTGGATG+AGG 0.489666 2.3:-73233566 MS.gene002256:CDS
CAGAGTATGTGTCAAGATTG+AGG 0.492768 2.3:-73233456 MS.gene002256:CDS
ACAAAGTCGAAGCAATTAGT+TGG 0.498298 2.3:-73232833 MS.gene002256:CDS
ACAATAGCGAGGAATTTCTC+GGG 0.503579 2.3:-73232586 MS.gene002256:CDS
AGAGTATGTGTCAAGATTGA+GGG 0.513752 2.3:-73233455 MS.gene002256:CDS
GAATTAATGGGACCATTAGC+GGG 0.514356 2.3:+73233193 None:intergenic
TGATAAGATTGGTCCGCTGT+TGG 0.514998 2.3:-73232951 MS.gene002256:CDS
GATTGGTCCGCTGTTGGGTA+GGG 0.516250 2.3:-73232945 MS.gene002256:CDS
GGATGCCGCGGCTTCTGCAA+AGG 0.518753 2.3:-73233695 MS.gene002256:CDS
GCTGCGCCCAGTCAGTTCCT+CGG 0.520974 2.3:+73233413 None:intergenic
TGAATTAATGGGACCATTAG+CGG 0.525771 2.3:+73233192 None:intergenic
ATTAAGGAAGTTAAGGAAGG+AGG 0.530708 2.3:-73233503 MS.gene002256:CDS
GGTGGATGAGGAAGAAGAAA+AGG 0.532095 2.3:-73233554 MS.gene002256:CDS
TTCGAGTATTCAAGATGTCC+TGG 0.533881 2.3:+73233241 None:intergenic
AAAGGAAATGAGGTTGAAGA+TGG 0.535620 2.3:-73233883 MS.gene002256:CDS
AGATTGGTCCGCTGTTGGGT+AGG 0.539038 2.3:-73232946 MS.gene002256:CDS
TTCACCTCCTGAATTTGGAA+AGG 0.542160 2.3:-73233734 MS.gene002256:CDS
GCTCTTTGTACTTCTCCAGT+AGG 0.543723 2.3:-73232653 MS.gene002256:CDS
ATTATCACCTGAGACCCATC+GGG 0.550131 2.3:+73233025 None:intergenic
AGATGGAAAGGAAGAACAGT+TGG 0.550771 2.3:-73233815 MS.gene002256:CDS
TTGAGCTACTCTTCACAACC+AGG 0.551752 2.3:+73233272 None:intergenic
CAATACAACGGGGATAATGC+AGG 0.553209 2.3:+73232387 None:intergenic
AAAGAGGAAGCTAGATGGAA+AGG 0.553316 2.3:-73233827 MS.gene002256:CDS
TGATAATCTGAATACTGAAG+TGG 0.554817 2.3:-73232537 MS.gene002256:CDS
TTGTTAAGCGCTGGTGGTGA+TGG 0.557212 2.3:-73232752 MS.gene002256:CDS
AAAGCATCTTTCTTGCCCGA+TGG 0.557386 2.3:-73233040 MS.gene002256:CDS
TTCTTGCTGCTGGATTAGAC+CGG 0.558175 2.3:-73233141 MS.gene002256:CDS
TATTATCACCTGAGACCCAT+CGG 0.558404 2.3:+73233024 None:intergenic
AAATGAGGTTGAAGATGGGA+AGG 0.558756 2.3:-73233878 MS.gene002256:CDS
AAGGAAATGAGGTTGAAGAT+GGG 0.562237 2.3:-73233882 MS.gene002256:CDS
AGTAGCTCAAAATTGTTGCC+AGG 0.564255 2.3:-73233259 MS.gene002256:CDS
GGAGTTTGATGTTGATACTG+AGG 0.566399 2.3:-73233857 MS.gene002256:CDS
CTCTTTGTACTTCTCCAGTA+GGG 0.567092 2.3:-73232652 MS.gene002256:CDS
CAGATTAAGGAAGTTAAGGA+AGG 0.571312 2.3:-73233506 MS.gene002256:CDS
ACCTCCTGAATTTGGAAAGG+GGG 0.574743 2.3:-73233731 MS.gene002256:CDS
TGCTGTGAGCCGAGGTTACG+AGG 0.576924 2.3:-73233326 MS.gene002256:CDS
CATCCAGATCCTCGTAACCT+CGG 0.587962 2.3:+73233317 None:intergenic
AATACTAGCAATCTGTTCAA+GGG 0.593971 2.3:-73232486 MS.gene002256:CDS
GGAAGTTGTGAAAGGAAATG+AGG 0.594864 2.3:-73233893 MS.gene002256:CDS
TTTCTGATCTACTTCAGGCA+TGG 0.600646 2.3:+73232285 None:intergenic
GAAGCAGCTGACCATTTCGA+TGG 0.604108 2.3:+73233161 None:intergenic
ATACACAAAGGTGTAGATGA+AGG 0.605326 2.3:-73232692 MS.gene002256:CDS
CTTCAGTAAAAGCTAATGAA+CGG 0.605560 2.3:+73232786 None:intergenic
TGTGAATATTTACAATAGCG+AGG 0.608077 2.3:-73232597 MS.gene002256:CDS
AAAAGGGAGGAAGTTGTGAA+AGG 0.616065 2.3:-73233901 MS.gene002256:CDS
AATAAAGCTGCTGTGAGCCG+AGG 0.616455 2.3:-73233334 MS.gene002256:CDS
AGAAGCCTTTGCAGAAGCCG+CGG 0.617253 2.3:+73233690 None:intergenic
TCTCAGAACAAATGAAGACC+TGG 0.621951 2.3:-73233290 MS.gene002256:CDS
CTGGGCTAAAATCAATACAA+CGG 0.628701 2.3:+73232375 None:intergenic
ACAGCCATAAAACCACCACC+TGG 0.630227 2.3:+73232356 None:intergenic
TGATGATGAGGTGGATGCCG+CGG 0.633167 2.3:-73233707 MS.gene002256:CDS
AAAGGAAGAACAGTTGGCAA+AGG 0.637301 2.3:-73233809 MS.gene002256:CDS
CACCTCCTGAATTTGGAAAG+GGG 0.637398 2.3:-73233732 MS.gene002256:CDS
GCTCAACCGAGGAACTGACT+GGG 0.639373 2.3:-73233419 MS.gene002256:CDS
CCAGTTAGAGAAAACAGTGT+AGG 0.639938 2.3:+73232427 None:intergenic
GTTGGTACTCTGAAGATGAA+CGG 0.640176 2.3:-73232815 MS.gene002256:CDS
TTAGAGAAAACAGTGTAGGA+TGG 0.640295 2.3:+73232431 None:intergenic
CAATAGCGAGGAATTTCTCG+GGG 0.640898 2.3:-73232585 MS.gene002256:CDS
GAAAAGAAAGCCGGTGTGGG+TGG 0.643363 2.3:-73233572 MS.gene002256:CDS
TGCAGGCTCGCATTTGAACG+CGG 0.645712 2.3:+73232404 None:intergenic
ACCATTGGGATCTGAGAACA+AGG 0.646289 2.3:-73232721 MS.gene002256:CDS
TCTTCCTCATCCACCCACAC+CGG 0.648855 2.3:+73233562 None:intergenic
CTTTGAAAAGAAAGCCGGTG+TGG 0.649038 2.3:-73233576 MS.gene002256:CDS
TTCTCTTCCCTACCCAACAG+CGG 0.649632 2.3:+73232938 None:intergenic
TGATGCTTTGAAAAGAAAGC+CGG 0.651576 2.3:-73233581 MS.gene002256:CDS
CAGCCATAAAACCACCACCT+GGG 0.668450 2.3:+73232357 None:intergenic
CATCTTCTTGGTAAACAGTG+AGG 0.681529 2.3:+73233632 None:intergenic
TGAGGAGACTATCTGCAGAA+CGG 0.693456 2.3:+73233650 None:intergenic
TTTGAAAAGAAAGCCGGTGT+GGG 0.693980 2.3:-73233575 MS.gene002256:CDS
ACAAGGACGTGTATACACAA+AGG 0.712046 2.3:-73232704 MS.gene002256:CDS
CAGCATGCAAAGCTCAACCG+AGG 0.712589 2.3:-73233430 MS.gene002256:CDS
TCCTTGTTCTCAGATCCCAA+TGG 0.723713 2.3:+73232720 None:intergenic
AATAGCGAGGAATTTCTCGG+GGG 0.758971 2.3:-73232584 MS.gene002256:CDS
GGGCTAAAATCAATACAACG+GGG 0.814054 2.3:+73232377 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTAAATTTTTTCAAATTGA+TGG - chr2.3:73233126-73233145 MS.gene002256:CDS 15.0%
!! TTTGAAAAAATTTAAGCTTC+CGG + chr2.3:73233122-73233141 None:intergenic 20.0%
!!! TTATAGTCTTGATTTAGTTA+AGG - chr2.3:73233260-73233279 MS.gene002256:CDS 20.0%
! TTCAGTATTCAGATTATCAA+TGG + chr2.3:73233703-73233722 None:intergenic 25.0%
!! CAATGAAGGTTATATTTTAC+TGG - chr2.3:73233380-73233399 MS.gene002256:CDS 25.0%
!!! TTTTTCAAATTGATGGCAAA+CGG - chr2.3:73233133-73233152 MS.gene002256:CDS 25.0%
AAAGCTATCATTTGAGAATC+AGG + chr2.3:73233354-73233373 None:intergenic 30.0%
AAATACTAGCAATCTGTTCA+AGG - chr2.3:73233754-73233773 MS.gene002256:CDS 30.0%
AAGGAAGATACTTTCAATCT+TGG + chr2.3:73233163-73233182 None:intergenic 30.0%
AATACTAGCAATCTGTTCAA+GGG - chr2.3:73233755-73233774 MS.gene002256:CDS 30.0%
ATGGAACTGAACTGAATTAA+TGG + chr2.3:73233064-73233083 None:intergenic 30.0%
CTTCAGTAAAAGCTAATGAA+CGG + chr2.3:73233458-73233477 None:intergenic 30.0%
TCAACAGATTAAGGAAGTTA+AGG - chr2.3:73232731-73232750 MS.gene002256:CDS 30.0%
TGATAATCTGAATACTGAAG+TGG - chr2.3:73233704-73233723 MS.gene002256:CDS 30.0%
TGATGGTCATATTTACCATT+GGG - chr2.3:73233506-73233525 MS.gene002256:CDS 30.0%
TGGAACTGAACTGAATTAAT+GGG + chr2.3:73233063-73233082 None:intergenic 30.0%
TGTGAATATTTACAATAGCG+AGG - chr2.3:73233644-73233663 MS.gene002256:CDS 30.0%
! AAGAAAGATGCTTTTCGAAT+GGG + chr2.3:73233195-73233214 None:intergenic 30.0%
! AGATTATCAATGGTCTTGAT+AGG + chr2.3:73233693-73233712 None:intergenic 30.0%
! CTCTAAAAATGAGACGAAAA+GGG - chr2.3:73232324-73232343 MS.gene002256:CDS 30.0%
! TCTCAAATGATAGCTTTTGT+GGG - chr2.3:73233357-73233376 MS.gene002256:CDS 30.0%
! TTCTCAAATGATAGCTTTTG+TGG - chr2.3:73233356-73233375 MS.gene002256:CDS 30.0%
!!! TCATTAGCTTTTACTGAAGA+TGG - chr2.3:73233459-73233478 MS.gene002256:CDS 30.0%
AAAGGAAATGAGGTTGAAGA+TGG - chr2.3:73232358-73232377 MS.gene002256:CDS 35.0%
AAGGAAATGAGGTTGAAGAT+GGG - chr2.3:73232359-73232378 MS.gene002256:CDS 35.0%
AATATACAAGATCCCGCTAA+TGG - chr2.3:73233036-73233055 MS.gene002256:CDS 35.0%
ACAAAGTCGAAGCAATTAGT+TGG - chr2.3:73233408-73233427 MS.gene002256:CDS 35.0%
AGAGTATGTGTCAAGATTGA+GGG - chr2.3:73232786-73232805 MS.gene002256:CDS 35.0%
ATACTGAGGAAACGAAAAAG+AGG - chr2.3:73232398-73232417 MS.gene002256:CDS 35.0%
ATTAAGGAAGTTAAGGAAGG+AGG - chr2.3:73232738-73232757 MS.gene002256:CDS 35.0%
CAGATTAAGGAAGTTAAGGA+AGG - chr2.3:73232735-73232754 MS.gene002256:CDS 35.0%
CTGGGCTAAAATCAATACAA+CGG + chr2.3:73233869-73233888 None:intergenic 35.0%
GTAGATGAAGGCTGTTTAAA+CGG - chr2.3:73233561-73233580 MS.gene002256:CDS 35.0%
GTGATGGTCATATTTACCAT+TGG - chr2.3:73233505-73233524 MS.gene002256:CDS 35.0%
TAAAAATGAGACGAAAAGGG+AGG - chr2.3:73232327-73232346 MS.gene002256:CDS 35.0%
TACAATAGCGAGGAATTTCT+CGG - chr2.3:73233654-73233673 MS.gene002256:CDS 35.0%
TAGCCAAAGTCAACAGATTA+AGG - chr2.3:73232722-73232741 MS.gene002256:CDS 35.0%
TGAATTAATGGGACCATTAG+CGG + chr2.3:73233052-73233071 None:intergenic 35.0%
TGGGCTAAAATCAATACAAC+GGG + chr2.3:73233868-73233887 None:intergenic 35.0%
TTAGAGAAAACAGTGTAGGA+TGG + chr2.3:73233813-73233832 None:intergenic 35.0%
! AAGCTTGAGAGCTTTTTGTT+TGG - chr2.3:73232475-73232494 MS.gene002256:CDS 35.0%
! AGAAAGATGCTTTTCGAATG+GGG + chr2.3:73233194-73233213 None:intergenic 35.0%
! ATACACAAAGGTGTAGATGA+AGG - chr2.3:73233549-73233568 MS.gene002256:CDS 35.0%
! CAAGAAAGATGCTTTTCGAA+TGG + chr2.3:73233196-73233215 None:intergenic 35.0%
!! AAGGCTAGAGTTGATAAGAT+TGG - chr2.3:73233279-73233298 MS.gene002256:CDS 35.0%
!! AGCTTGAGAGCTTTTTGTTT+GGG - chr2.3:73232476-73232495 MS.gene002256:CDS 35.0%
!! CTTCCTTAATCTGTTGACTT+TGG + chr2.3:73232728-73232747 None:intergenic 35.0%
!! GAAGAGTATTGAAGTGTTTG+AGG - chr2.3:73233323-73233342 MS.gene002256:CDS 35.0%
!! GCTCTAAAAATGAGACGAAA+AGG - chr2.3:73232323-73232342 MS.gene002256:CDS 35.0%
!! TGATGCTTTGAAAAGAAAGC+CGG - chr2.3:73232660-73232679 MS.gene002256:CDS 35.0%
AAAAGGGAGGAAGTTGTGAA+AGG - chr2.3:73232340-73232359 MS.gene002256:CDS 40.0%
AAAGAGGAAGCTAGATGGAA+AGG - chr2.3:73232414-73232433 MS.gene002256:CDS 40.0%
AAAGGAAGAACAGTTGGCAA+AGG - chr2.3:73232432-73232451 MS.gene002256:CDS 40.0%
AAATGAGGTTGAAGATGGGA+AGG - chr2.3:73232363-73232382 MS.gene002256:CDS 40.0%
ACAAGGACGTGTATACACAA+AGG - chr2.3:73233537-73233556 MS.gene002256:CDS 40.0%
ACAATAGCGAGGAATTTCTC+GGG - chr2.3:73233655-73233674 MS.gene002256:CDS 40.0%
ACGAAAAAGAGGAAGCTAGA+TGG - chr2.3:73232409-73232428 MS.gene002256:CDS 40.0%
AGATGGAAAGGAAGAACAGT+TGG - chr2.3:73232426-73232445 MS.gene002256:CDS 40.0%
AGTAGCTCAAAATTGTTGCC+AGG - chr2.3:73232982-73233001 MS.gene002256:CDS 40.0%
CAGAGTATGTGTCAAGATTG+AGG - chr2.3:73232785-73232804 MS.gene002256:CDS 40.0%
CATCTTCTTGGTAAACAGTG+AGG + chr2.3:73232612-73232631 None:intergenic 40.0%
CCAGTTAGAGAAAACAGTGT+AGG + chr2.3:73233817-73233836 None:intergenic 40.0%
CTCTATTCACCTCCTGAATT+TGG - chr2.3:73232502-73232521 MS.gene002256:CDS 40.0%
CTCTTTGTACTTCTCCAGTA+GGG - chr2.3:73233589-73233608 MS.gene002256:CDS 40.0%
GAATTAATGGGACCATTAGC+GGG + chr2.3:73233051-73233070 None:intergenic 40.0%
GAGGATGAGGATTTGATATC+TGG - chr2.3:73232760-73232779 MS.gene002256:CDS 40.0%
GATTCAGGGTCAAAAATGGA+TGG - chr2.3:73232853-73232872 MS.gene002256:CDS 40.0%
GGAAGTTGTGAAAGGAAATG+AGG - chr2.3:73232348-73232367 MS.gene002256:CDS 40.0%
GGACAAAAATTGTTAAGCGC+TGG - chr2.3:73233480-73233499 MS.gene002256:CDS 40.0%
GGAGTTTGATGTTGATACTG+AGG - chr2.3:73232384-73232403 MS.gene002256:CDS 40.0%
GGGCTAAAATCAATACAACG+GGG + chr2.3:73233867-73233886 None:intergenic 40.0%
TATTATCACCTGAGACCCAT+CGG + chr2.3:73233220-73233239 None:intergenic 40.0%
TCACCTCCTGAATTTGGAAA+GGG - chr2.3:73232508-73232527 MS.gene002256:CDS 40.0%
TCAGTTCAGTTCCATCGAAA+TGG - chr2.3:73233069-73233088 MS.gene002256:CDS 40.0%
TCTCAGAACAAATGAAGACC+TGG - chr2.3:73232951-73232970 MS.gene002256:CDS 40.0%
TTCACCTCCTGAATTTGGAA+AGG - chr2.3:73232507-73232526 MS.gene002256:CDS 40.0%
TTCGAGTATTCAAGATGTCC+TGG + chr2.3:73233003-73233022 None:intergenic 40.0%
TTTGAAAAGAAAGCCGGTGT+GGG - chr2.3:73232666-73232685 MS.gene002256:CDS 40.0%
! ATAGCTTTTGTGGGCAATGA+AGG - chr2.3:73233366-73233385 MS.gene002256:CDS 40.0%
! CCTACACTGTTTTCTCTAAC+TGG - chr2.3:73233814-73233833 MS.gene002256:CDS 40.0%
! GTTGGTACTCTGAAGATGAA+CGG - chr2.3:73233426-73233445 MS.gene002256:CDS 40.0%
!! CAAAAATTGTTAAGCGCTGG+TGG - chr2.3:73233483-73233502 MS.gene002256:CDS 40.0%
!!! CGTTGTATTGATTTTAGCCC+AGG - chr2.3:73233867-73233886 MS.gene002256:CDS 40.0%
!!! TGTATTGATTTTAGCCCAGG+TGG - chr2.3:73233870-73233889 MS.gene002256:CDS 40.0%
AAGCATCTTTCTTGCCCGAT+GGG - chr2.3:73233202-73233221 MS.gene002256:CDS 45.0%
AAGGAAGTTAAGGAAGGAGG+AGG - chr2.3:73232741-73232760 MS.gene002256:CDS 45.0%
AATAGCGAGGAATTTCTCGG+GGG - chr2.3:73233657-73233676 MS.gene002256:CDS 45.0%
ACCATTGGGATCTGAGAACA+AGG - chr2.3:73233520-73233539 MS.gene002256:CDS 45.0%
ACCTCCTGAATTTGGAAAGG+GGG - chr2.3:73232510-73232529 MS.gene002256:CDS 45.0%
ATGGCTGTAGGAAATGCTTC+AGG - chr2.3:73233900-73233919 MS.gene002256:CDS 45.0%
ATTATCACCTGAGACCCATC+GGG + chr2.3:73233219-73233238 None:intergenic 45.0%
CAATACAACGGGGATAATGC+AGG + chr2.3:73233857-73233876 None:intergenic 45.0%
CAATAGCGAGGAATTTCTCG+GGG - chr2.3:73233656-73233675 MS.gene002256:CDS 45.0%
CACCTCCTGAATTTGGAAAG+GGG - chr2.3:73232509-73232528 MS.gene002256:CDS 45.0%
CTGAGAAGTCAGCATCTTCT+TGG + chr2.3:73232624-73232643 None:intergenic 45.0%
GCTCTTTGTACTTCTCCAGT+AGG - chr2.3:73233588-73233607 MS.gene002256:CDS 45.0%
GCTGGATTCAGGGTCAAAAA+TGG - chr2.3:73232849-73232868 MS.gene002256:CDS 45.0%
GGGTCTCAGGTGATAATATC+AGG - chr2.3:73233222-73233241 MS.gene002256:CDS 45.0%
GGTGGATGAGGAAGAAGAAA+AGG - chr2.3:73232687-73232706 MS.gene002256:CDS 45.0%
TCATCCCCCTTTCCAAATTC+AGG + chr2.3:73232517-73232536 None:intergenic 45.0%
TCCTTGTTCTCAGATCCCAA+TGG + chr2.3:73233524-73233543 None:intergenic 45.0%
TGAGGAGACTATCTGCAGAA+CGG + chr2.3:73232594-73232613 None:intergenic 45.0%
TTCGAATGGGGCAATCTTCA+AGG + chr2.3:73233182-73233201 None:intergenic 45.0%
TTGAGCTACTCTTCACAACC+AGG + chr2.3:73232972-73232991 None:intergenic 45.0%
! AAAGCATCTTTCTTGCCCGA+TGG - chr2.3:73233201-73233220 MS.gene002256:CDS 45.0%
! CTTTGAAAAGAAAGCCGGTG+TGG - chr2.3:73232665-73232684 MS.gene002256:CDS 45.0%
! GATAAGATTGGTCCGCTGTT+GGG - chr2.3:73233291-73233310 MS.gene002256:CDS 45.0%
! TGATAAGATTGGTCCGCTGT+TGG - chr2.3:73233290-73233309 MS.gene002256:CDS 45.0%
! TTCTTGCTGCTGGATTAGAC+CGG - chr2.3:73233100-73233119 MS.gene002256:CDS 45.0%
!! ATTGATTTTAGCCCAGGTGG+TGG - chr2.3:73233873-73233892 MS.gene002256:CDS 45.0%
AATAAAGCTGCTGTGAGCCG+AGG - chr2.3:73232907-73232926 MS.gene002256:CDS 50.0%
ACAGCCATAAAACCACCACC+TGG + chr2.3:73233888-73233907 None:intergenic 50.0%
CAGCCATAAAACCACCACCT+GGG + chr2.3:73233887-73233906 None:intergenic 50.0%
CATCCAGATCCTCGTAACCT+CGG + chr2.3:73232927-73232946 None:intergenic 50.0%
GAAGCAGCTGACCATTTCGA+TGG + chr2.3:73233083-73233102 None:intergenic 50.0%
GCTGCAAAATGTGTCCCTAC+TGG + chr2.3:73233606-73233625 None:intergenic 50.0%
GTTAAGGAAGGAGGAGGATG+AGG - chr2.3:73232747-73232766 MS.gene002256:CDS 50.0%
TCCCCCTTTCCAAATTCAGG+AGG + chr2.3:73232514-73232533 None:intergenic 50.0%
TTCTCTTCCCTACCCAACAG+CGG + chr2.3:73233306-73233325 None:intergenic 50.0%
! AAAGGGGGATGATGATGAGG+TGG - chr2.3:73232525-73232544 MS.gene002256:CDS 50.0%
! GTAGGGACACATTTTGCAGC+TGG - chr2.3:73233606-73233625 MS.gene002256:CDS 50.0%
! TGGAAAGGGGGATGATGATG+AGG - chr2.3:73232522-73232541 MS.gene002256:CDS 50.0%
! TTTGCAGCTGGTTCAGCTAG+TGG - chr2.3:73233618-73233637 MS.gene002256:CDS 50.0%
!! TAGCCCAGGTGGTGGTTTTA+TGG - chr2.3:73233881-73233900 MS.gene002256:CDS 50.0%
!! TTGTTAAGCGCTGGTGGTGA+TGG - chr2.3:73233489-73233508 MS.gene002256:CDS 50.0%
!!! GGTGGTGGTTTTATGGCTGT+AGG - chr2.3:73233888-73233907 MS.gene002256:CDS 50.0%
AGAAGCCTTTGCAGAAGCCG+CGG + chr2.3:73232554-73232573 None:intergenic 55.0%
AGCTCAACCGAGGAACTGAC+TGG - chr2.3:73232821-73232840 MS.gene002256:CDS 55.0%
CAGCATGCAAAGCTCAACCG+AGG - chr2.3:73232811-73232830 MS.gene002256:CDS 55.0%
GAAAAGAAAGCCGGTGTGGG+TGG - chr2.3:73232669-73232688 MS.gene002256:CDS 55.0%
GCTCAACCGAGGAACTGACT+GGG - chr2.3:73232822-73232841 MS.gene002256:CDS 55.0%
TCTTCCTCATCCACCCACAC+CGG + chr2.3:73232682-73232701 None:intergenic 55.0%
TGCAGGCTCGCATTTGAACG+CGG + chr2.3:73233840-73233859 None:intergenic 55.0%
TTTCTTGCCCGATGGGTCTC+AGG - chr2.3:73233209-73233228 MS.gene002256:CDS 55.0%
! AGATTGGTCCGCTGTTGGGT+AGG - chr2.3:73233295-73233314 MS.gene002256:CDS 55.0%
! GATTGGTCCGCTGTTGGGTA+GGG - chr2.3:73233296-73233315 MS.gene002256:CDS 55.0%
! TGATGATGAGGTGGATGCCG+CGG - chr2.3:73232534-73232553 MS.gene002256:CDS 55.0%
AAAGCCGGTGTGGGTGGATG+AGG - chr2.3:73232675-73232694 MS.gene002256:CDS 60.0%
ACTGGGCGCAGCTGGATTCA+GGG - chr2.3:73232839-73232858 MS.gene002256:CDS 60.0%
GAGCCGAGGTTACGAGGATC+TGG - chr2.3:73232921-73232940 MS.gene002256:CDS 60.0%
GGTCAGCTGCTTCTTGCTGC+TGG - chr2.3:73233090-73233109 MS.gene002256:CDS 60.0%
TGCTGTGAGCCGAGGTTACG+AGG - chr2.3:73232915-73232934 MS.gene002256:CDS 60.0%
AGGAACTGACTGGGCGCAGC+TGG - chr2.3:73232831-73232850 MS.gene002256:CDS 65.0%
GACTGGGCGCAGCTGGATTC+AGG - chr2.3:73232838-73232857 MS.gene002256:CDS 65.0%
GCTGCGCCCAGTCAGTTCCT+CGG + chr2.3:73232831-73232850 None:intergenic 65.0%
GGATGCCGCGGCTTCTGCAA+AGG - chr2.3:73232546-73232565 MS.gene002256:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 73232298 73233965 73232298 ID=MS.gene002256
chr2.3 mRNA 73232298 73233965 73232298 ID=MS.gene002256.t1;Parent=MS.gene002256
chr2.3 exon 73232298 73233965 73232298 ID=MS.gene002256.t1.exon1;Parent=MS.gene002256.t1
chr2.3 CDS 73232298 73233965 73232298 ID=cds.MS.gene002256.t1;Parent=MS.gene002256.t1
Gene Sequence

>MS.gene002256

ATGAGTTTAATATCTCAAAATGCTCGCTCTAAAAATGAGACGAAAAGGGAGGAAGTTGTGAAAGGAAATGAGGTTGAAGATGGGAAGGAGTTTGATGTTGATACTGAGGAAACGAAAAAGAGGAAGCTAGATGGAAAGGAAGAACAGTTGGCAAAGGAACAAGTTAAAGAAATGAAAAAGCTTGAGAGCTTTTTGTTTGGGTCTCTCTATTCACCTCCTGAATTTGGAAAGGGGGATGATGATGAGGTGGATGCCGCGGCTTCTGCAAAGGCTTCTAATTTGTTCTTCACTGACCGTTCTGCAGATAGTCTCCTCACTGTTTACCAAGAAGATGCTGACTTCTCAGATGAAAGTGATAATGATGATGCTTTGAAAAGAAAGCCGGTGTGGGTGGATGAGGAAGAAGAAAAGGTTACTGTAAACATAGCCAAAGTCAACAGATTAAGGAAGTTAAGGAAGGAGGAGGATGAGGATTTGATATCTGGTTCAGAGTATGTGTCAAGATTGAGGGCACAGCATGCAAAGCTCAACCGAGGAACTGACTGGGCGCAGCTGGATTCAGGGTCAAAAATGGATGGATCTTCTGATGATGAATTGACAGATGATGAAAATAAAGCTGCTGTGAGCCGAGGTTACGAGGATCTGGATGATATTCTCAGAACAAATGAAGACCTGGTTGTGAAGAGTAGCTCAAAATTGTTGCCAGGACATCTTGAATACTCGAAGCTTGTCGATGCAAATATACAAGATCCCGCTAATGGTCCCATTAATTCAGTTCAGTTCCATCGAAATGGTCAGCTGCTTCTTGCTGCTGGATTAGACCGGAAGCTTAAATTTTTTCAAATTGATGGCAAACGGAACACCAAGATTGAAAGTATCTTCCTTGAAGATTGCCCCATTCGAAAAGCATCTTTCTTGCCCGATGGGTCTCAGGTGATAATATCAGGAAGAAGAAAGTTCTTTTATAGTCTTGATTTAGTTAAGGCTAGAGTTGATAAGATTGGTCCGCTGTTGGGTAGGGAAGAGAAGAGTATTGAAGTGTTTGAGGTCTCTCCTGATTCTCAAATGATAGCTTTTGTGGGCAATGAAGGTTATATTTTACTGGTTTCAACAAAGTCGAAGCAATTAGTTGGTACTCTGAAGATGAACGGAACAGTCCGTTCATTAGCTTTTACTGAAGATGGACAAAAATTGTTAAGCGCTGGTGGTGATGGTCATATTTACCATTGGGATCTGAGAACAAGGACGTGTATACACAAAGGTGTAGATGAAGGCTGTTTAAACGGCACAGCTCTTTGTACTTCTCCAGTAGGGACACATTTTGCAGCTGGTTCAGCTAGTGGAGTTGTGAATATTTACAATAGCGAGGAATTTCTCGGGGGAAAGAGAAAGCCTATCAAGACCATTGATAATCTGAATACTGAAGTGGATTTCATGAAATTCAATCATGACTCTCAAATACTAGCAATCTGTTCAAGGGCGAAGAACAGCAGTTTGAAATTAGTACATATACCATCCTACACTGTTTTCTCTAACTGGCCGCGTTCAAATGCGAGCCTGCATTATCCCCGTTGTATTGATTTTAGCCCAGGTGGTGGTTTTATGGCTGTAGGAAATGCTTCAGGCAAAGTGTTACTGTATAAGTTGCACCATTATCACCATGCCTGA
Protein sequence

>MS.gene002256.t1

MSLISQNARSKNETKREEVVKGNEVEDGKEFDVDTEETKKRKLDGKEEQLAKEQVKEMKKLESFLFGSLYSPPEFGKGDDDEVDAAASAKASNLFFTDRSADSLLTVYQEDADFSDESDNDDALKRKPVWVDEEEEKVTVNIAKVNRLRKLRKEEDEDLISGSEYVSRLRAQHAKLNRGTDWAQLDSGSKMDGSSDDELTDDENKAAVSRGYEDLDDILRTNEDLVVKSSSKLLPGHLEYSKLVDANIQDPANGPINSVQFHRNGQLLLAAGLDRKLKFFQIDGKRNTKIESIFLEDCPIRKASFLPDGSQVIISGRRKFFYSLDLVKARVDKIGPLLGREEKSIEVFEVSPDSQMIAFVGNEGYILLVSTKSKQLVGTLKMNGTVRSLAFTEDGQKLLSAGGDGHIYHWDLRTRTCIHKGVDEGCLNGTALCTSPVGTHFAAGSASGVVNIYNSEEFLGGKRKPIKTIDNLNTEVDFMKFNHDSQILAICSRAKNSSLKLVHIPSYTVFSNWPRSNASLHYPRCIDFSPGGGFMAVGNASGKVLLYKLHHYHHA