Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05721.t1 | XP_003600436.2 | 100 | 216 | 0 | 0 | 6 | 221 | 1 | 216 | 4.70E-116 | 427.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05721.t1 | A7RP64 | 62.8 | 196 | 73 | 0 | 26 | 221 | 43 | 238 | 7.8e-67 | 255.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05721.t1 | I3S062 | 100.0 | 216 | 0 | 0 | 6 | 221 | 1 | 216 | 3.4e-116 | 427.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05721 | MS.gene08073 | 0.855984 | 4.37E-62 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05721.t1 | MTR_3g061130 | 100.000 | 216 | 0 | 0 | 6 | 221 | 1 | 216 | 8.19e-162 | 446 |
| MS.gene05721.t1 | MTR_5g088410 | 60.465 | 215 | 52 | 5 | 8 | 221 | 6 | 188 | 1.11e-73 | 221 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05721.t1 | AT3G13230 | 75.472 | 212 | 52 | 0 | 8 | 219 | 4 | 215 | 6.72e-123 | 347 |
| MS.gene05721.t1 | AT3G13230 | 75.472 | 212 | 52 | 0 | 8 | 219 | 4 | 215 | 6.72e-123 | 347 |
Find 48 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTCTTTGTTACCTAATTA+TGG | 0.104484 | 3.2:+52109846 | MS.gene05721:CDS |
| AGATCCCAGAATGTGAATTT+TGG | 0.238915 | 3.2:-52109797 | None:intergenic |
| TGATACCAAAATTCACATTC+TGG | 0.283493 | 3.2:+52109792 | MS.gene05721:CDS |
| CTGCAGGTGATCCCATAATT+AGG | 0.290266 | 3.2:-52109858 | None:intergenic |
| AGTTGAGAGCCGTTACTTCT+AGG | 0.295245 | 3.2:+52109896 | MS.gene05721:CDS |
| TGCTATTGCTATTCTGCGTT+TGG | 0.312006 | 3.2:+52109621 | MS.gene05721:CDS |
| TCTCTTTGTTACCTAATTAT+GGG | 0.325990 | 3.2:+52109847 | MS.gene05721:CDS |
| TTTGTTCATGCTTTCATGTT+GGG | 0.347801 | 3.2:+52109583 | MS.gene05721:CDS |
| GGCTCAAACTTTGGCTTTGG+AGG | 0.352039 | 3.2:-52109340 | None:intergenic |
| AGAGGCTCAAACTTTGGCTT+TGG | 0.356673 | 3.2:-52109343 | None:intergenic |
| ATACACCTCTCAAGAAAGTT+TGG | 0.369468 | 3.2:+52109431 | MS.gene05721:CDS |
| GATCACTTGTCCCGTGCTAT+TGG | 0.373903 | 3.2:+52109697 | MS.gene05721:CDS |
| CGTGCTATTGGAAGATTATC+TGG | 0.376662 | 3.2:+52109709 | MS.gene05721:CDS |
| TGCATTCTCAATTGTAAACT+TGG | 0.386651 | 3.2:-52109746 | None:intergenic |
| GGTTTCAGAGGCTCAAACTT+TGG | 0.395402 | 3.2:-52109349 | None:intergenic |
| TTCGACCCTTCAAATTCATT+CGG | 0.417898 | 3.2:-52109498 | None:intergenic |
| TGTTGAGTCCTTTGAGATCA+AGG | 0.427401 | 3.2:+52109654 | MS.gene05721:CDS |
| CACAATGGCATCATCTTCAA+TGG | 0.462021 | 3.2:+52109282 | MS.gene05721:CDS |
| ATTGGAAGATTATCTGGAAA+AGG | 0.469137 | 3.2:+52109715 | MS.gene05721:CDS |
| TTGCAAACATCAAGATTGCT+AGG | 0.471818 | 3.2:+52109821 | MS.gene05721:CDS |
| AGAAGTAACGGCTCTCAACT+TGG | 0.482912 | 3.2:-52109893 | None:intergenic |
| TGACATCCGAATGAATTTGA+AGG | 0.495230 | 3.2:+52109492 | MS.gene05721:CDS |
| CGAAACTGAACCGCAGCACC+AGG | 0.498813 | 3.2:-52109382 | None:intergenic |
| TTGGAATATACCTTTCCTGC+AGG | 0.500426 | 3.2:-52109874 | None:intergenic |
| AAGGATGTTAAGACTCTTCG+AGG | 0.505057 | 3.2:+52109673 | MS.gene05721:CDS |
| GATACCAAAATTCACATTCT+GGG | 0.511425 | 3.2:+52109793 | MS.gene05721:CDS |
| CTAATTATGGGATCACCTGC+AGG | 0.512370 | 3.2:+52109859 | MS.gene05721:CDS |
| CTGAAACCTCATGAGATGCC+TGG | 0.516679 | 3.2:+52109364 | MS.gene05721:CDS |
| TGCAAACATCAAGATTGCTA+GGG | 0.524357 | 3.2:+52109822 | MS.gene05721:CDS |
| TATGGGATCACCTGCAGGAA+AGG | 0.544799 | 3.2:+52109864 | MS.gene05721:CDS |
| CAGATAATCTTCCAATAGCA+CGG | 0.551311 | 3.2:-52109708 | None:intergenic |
| GACATCCGAATGAATTTGAA+GGG | 0.559638 | 3.2:+52109493 | MS.gene05721:CDS |
| CTCTTTAAACAGTTGCACAA+TGG | 0.561554 | 3.2:+52109267 | MS.gene05721:intron |
| ACCTCTCAAGAAAGTTTGGA+TGG | 0.563023 | 3.2:+52109435 | MS.gene05721:CDS |
| TCATGAGATGCCTGGTGCTG+CGG | 0.567566 | 3.2:+52109372 | MS.gene05721:CDS |
| TCCATCCAAACTTTCTTGAG+AGG | 0.572045 | 3.2:-52109436 | None:intergenic |
| GGAAGATTATCTGGAAAAGG+TGG | 0.573573 | 3.2:+52109718 | MS.gene05721:CDS |
| TTCTGCCATCCTAGAAGTAA+CGG | 0.576020 | 3.2:-52109905 | None:intergenic |
| GAGAGCCGTTACTTCTAGGA+TGG | 0.578516 | 3.2:+52109900 | MS.gene05721:CDS |
| TTACTTCTAGGATGGCAGAA+AGG | 0.584575 | 3.2:+52109908 | MS.gene05721:CDS |
| AGATAATCTTCCAATAGCAC+GGG | 0.591480 | 3.2:-52109707 | None:intergenic |
| TGCTGCGGTTCAGTTTCGCA+AGG | 0.601400 | 3.2:+52109387 | MS.gene05721:CDS |
| GGCATCTCATGAGGTTTCAG+AGG | 0.608176 | 3.2:-52109361 | None:intergenic |
| CTTAACATCCTTGATCTCAA+AGG | 0.609805 | 3.2:-52109662 | None:intergenic |
| GCAGCACCAGGCATCTCATG+AGG | 0.646562 | 3.2:-52109370 | None:intergenic |
| TTGAGAGGTGTATAACGATG+TGG | 0.685518 | 3.2:-52109421 | None:intergenic |
| GAAAAGTTGAGTTGAAGACA+AGG | 0.702510 | 3.2:+52109518 | MS.gene05721:CDS |
| AGAGGTGTATAACGATGTGG+TGG | 0.702702 | 3.2:-52109418 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACTTTACATACATATATG+AGG | + | chr3.2:52109068-52109087 | MS.gene05721:intron | 20.0% |
| ! | TCTCTTTGTTACCTAATTAT+GGG | + | chr3.2:52109847-52109866 | MS.gene05721:CDS | 25.0% |
| ! | TTCTCTTTGTTACCTAATTA+TGG | + | chr3.2:52109846-52109865 | MS.gene05721:CDS | 25.0% |
| !! | AATTTTCATCTGCTCAAATA+CGG | - | chr3.2:52109473-52109492 | None:intergenic | 25.0% |
| ATTGGAAGATTATCTGGAAA+AGG | + | chr3.2:52109715-52109734 | MS.gene05721:CDS | 30.0% | |
| GATACCAAAATTCACATTCT+GGG | + | chr3.2:52109793-52109812 | MS.gene05721:CDS | 30.0% | |
| TGATACCAAAATTCACATTC+TGG | + | chr3.2:52109792-52109811 | MS.gene05721:CDS | 30.0% | |
| TGCATTCTCAATTGTAAACT+TGG | - | chr3.2:52109749-52109768 | None:intergenic | 30.0% | |
| TTTGTTCATGCTTTCATGTT+GGG | + | chr3.2:52109583-52109602 | MS.gene05721:CDS | 30.0% | |
| ! | ATTTTCATCTGCTCAAATAC+GGG | - | chr3.2:52109472-52109491 | None:intergenic | 30.0% |
| ! | GCAAAACTTTTTGTTCAGAT+AGG | - | chr3.2:52109321-52109340 | None:intergenic | 30.0% |
| ! | TTTTGTTCATGCTTTCATGT+TGG | + | chr3.2:52109582-52109601 | MS.gene05721:CDS | 30.0% |
| ACATGTTCCCTGAACTATTT+AGG | + | chr3.2:52109174-52109193 | MS.gene05721:intron | 35.0% | |
| AGATAATCTTCCAATAGCAC+GGG | - | chr3.2:52109710-52109729 | None:intergenic | 35.0% | |
| ATACACCTCTCAAGAAAGTT+TGG | + | chr3.2:52109431-52109450 | MS.gene05721:CDS | 35.0% | |
| ATAGTTCAGGGAACATGTTA+TGG | - | chr3.2:52109172-52109191 | None:intergenic | 35.0% | |
| ATCTACCACCTAAATAGTTC+AGG | - | chr3.2:52109185-52109204 | None:intergenic | 35.0% | |
| CAGATAATCTTCCAATAGCA+CGG | - | chr3.2:52109711-52109730 | None:intergenic | 35.0% | |
| CTCTTTAAACAGTTGCACAA+TGG | + | chr3.2:52109267-52109286 | MS.gene05721:intron | 35.0% | |
| CTTAACATCCTTGATCTCAA+AGG | - | chr3.2:52109665-52109684 | None:intergenic | 35.0% | |
| GACATCCGAATGAATTTGAA+GGG | + | chr3.2:52109493-52109512 | MS.gene05721:CDS | 35.0% | |
| TCTACCACCTAAATAGTTCA+GGG | - | chr3.2:52109184-52109203 | None:intergenic | 35.0% | |
| TGACATCCGAATGAATTTGA+AGG | + | chr3.2:52109492-52109511 | MS.gene05721:CDS | 35.0% | |
| TGCAAACATCAAGATTGCTA+GGG | + | chr3.2:52109822-52109841 | MS.gene05721:CDS | 35.0% | |
| TTCGACCCTTCAAATTCATT+CGG | - | chr3.2:52109501-52109520 | None:intergenic | 35.0% | |
| TTGCAAACATCAAGATTGCT+AGG | + | chr3.2:52109821-52109840 | MS.gene05721:CDS | 35.0% | |
| ! | AGATCCCAGAATGTGAATTT+TGG | - | chr3.2:52109800-52109819 | None:intergenic | 35.0% |
| ! | CAAAATCAGCACATTTTTGC+AGG | - | chr3.2:52109567-52109586 | None:intergenic | 35.0% |
| !! | ACAAAAGTGCTAATAGCTGT+AGG | + | chr3.2:52109128-52109147 | MS.gene05721:intron | 35.0% |
| !! | GAAAAGTTGAGTTGAAGACA+AGG | + | chr3.2:52109518-52109537 | MS.gene05721:CDS | 35.0% |
| AAGGATGTTAAGACTCTTCG+AGG | + | chr3.2:52109673-52109692 | MS.gene05721:CDS | 40.0% | |
| ACCTCTCAAGAAAGTTTGGA+TGG | + | chr3.2:52109435-52109454 | MS.gene05721:CDS | 40.0% | |
| CACAATGGCATCATCTTCAA+TGG | + | chr3.2:52109282-52109301 | MS.gene05721:CDS | 40.0% | |
| GGAAGATTATCTGGAAAAGG+TGG | + | chr3.2:52109718-52109737 | MS.gene05721:CDS | 40.0% | |
| TCCATCCAAACTTTCTTGAG+AGG | - | chr3.2:52109439-52109458 | None:intergenic | 40.0% | |
| TGTTCCCTGAACTATTTAGG+TGG | + | chr3.2:52109177-52109196 | MS.gene05721:intron | 40.0% | |
| TTCTGCCATCCTAGAAGTAA+CGG | - | chr3.2:52109908-52109927 | None:intergenic | 40.0% | |
| TTGGAATATACCTTTCCTGC+AGG | - | chr3.2:52109877-52109896 | None:intergenic | 40.0% | |
| ! | CGTGCTATTGGAAGATTATC+TGG | + | chr3.2:52109709-52109728 | MS.gene05721:CDS | 40.0% |
| ! | TGCTATTGCTATTCTGCGTT+TGG | + | chr3.2:52109621-52109640 | MS.gene05721:CDS | 40.0% |
| ! | TTGAGAGGTGTATAACGATG+TGG | - | chr3.2:52109424-52109443 | None:intergenic | 40.0% |
| ! | TTTTGCAGGTTACTGATGTC+AGG | - | chr3.2:52109553-52109572 | None:intergenic | 40.0% |
| !! | TGTTGAGTCCTTTGAGATCA+AGG | + | chr3.2:52109654-52109673 | MS.gene05721:CDS | 40.0% |
| !! | TTACTTCTAGGATGGCAGAA+AGG | + | chr3.2:52109908-52109927 | MS.gene05721:CDS | 40.0% |
| AGAAGTAACGGCTCTCAACT+TGG | - | chr3.2:52109896-52109915 | None:intergenic | 45.0% | |
| AGTTGAGAGCCGTTACTTCT+AGG | + | chr3.2:52109896-52109915 | MS.gene05721:CDS | 45.0% | |
| CTAATTATGGGATCACCTGC+AGG | + | chr3.2:52109859-52109878 | MS.gene05721:CDS | 45.0% | |
| CTGCAGGTGATCCCATAATT+AGG | - | chr3.2:52109861-52109880 | None:intergenic | 45.0% | |
| GGTTTCAGAGGCTCAAACTT+TGG | - | chr3.2:52109352-52109371 | None:intergenic | 45.0% | |
| ! | AGAGGCTCAAACTTTGGCTT+TGG | - | chr3.2:52109346-52109365 | None:intergenic | 45.0% |
| ! | AGAGGTGTATAACGATGTGG+TGG | - | chr3.2:52109421-52109440 | None:intergenic | 45.0% |
| CTGAAACCTCATGAGATGCC+TGG | + | chr3.2:52109364-52109383 | MS.gene05721:CDS | 50.0% | |
| GATCACTTGTCCCGTGCTAT+TGG | + | chr3.2:52109697-52109716 | MS.gene05721:CDS | 50.0% | |
| GGCATCTCATGAGGTTTCAG+AGG | - | chr3.2:52109364-52109383 | None:intergenic | 50.0% | |
| TATGGGATCACCTGCAGGAA+AGG | + | chr3.2:52109864-52109883 | MS.gene05721:CDS | 50.0% | |
| ! | GAGAGCCGTTACTTCTAGGA+TGG | + | chr3.2:52109900-52109919 | MS.gene05721:CDS | 50.0% |
| !! | GGCTCAAACTTTGGCTTTGG+AGG | - | chr3.2:52109343-52109362 | None:intergenic | 50.0% |
| TGCTGCGGTTCAGTTTCGCA+AGG | + | chr3.2:52109387-52109406 | MS.gene05721:CDS | 55.0% | |
| ! | TCATGAGATGCCTGGTGCTG+CGG | + | chr3.2:52109372-52109391 | MS.gene05721:CDS | 55.0% |
| CGAAACTGAACCGCAGCACC+AGG | - | chr3.2:52109385-52109404 | None:intergenic | 60.0% | |
| GCAGCACCAGGCATCTCATG+AGG | - | chr3.2:52109373-52109392 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 52109012 | 52109936 | 52109012 | ID=MS.gene05721 |
| chr3.2 | mRNA | 52109012 | 52109936 | 52109012 | ID=MS.gene05721.t1;Parent=MS.gene05721 |
| chr3.2 | exon | 52109012 | 52109019 | 52109012 | ID=MS.gene05721.t1.exon1;Parent=MS.gene05721.t1 |
| chr3.2 | CDS | 52109012 | 52109019 | 52109012 | ID=cds.MS.gene05721.t1;Parent=MS.gene05721.t1 |
| chr3.2 | exon | 52109279 | 52109936 | 52109279 | ID=MS.gene05721.t1.exon2;Parent=MS.gene05721.t1 |
| chr3.2 | CDS | 52109279 | 52109936 | 52109279 | ID=cds.MS.gene05721.t1;Parent=MS.gene05721.t1 |
| Gene Sequence |
| Protein sequence |
