AlfalfaGEDB | MS.gene20681

M. sativa cultivar XinJiangDaYe / MS.gene20681

KEGG: K14549
GO: GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016070 GO:0016072 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0046483 GO:0071704 GO:0090304 GO:0110165 GO:1901360
PFAM: PF00400.35 PF09384.13

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20681.t1	XP_003591585.1	97.4	530	14	0	1	530	1	530	6.10E-295	1022.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20681.t1	O82266	58.8	531	207	6	1	529	9	529	2.9e-168	593.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20681.t1	G7I2U5	97.4	530	14	0	1	530	1	530	4.4e-295	1022.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene053007	MS.gene20681	0.825899	3.64E-54	-1.69E-46
MS.gene056403	MS.gene20681	0.80447	2.13E-49	-1.69E-46
MS.gene056605	MS.gene20681	0.823499	1.34E-53	-1.69E-46
MS.gene05689	MS.gene20681	0.847351	1.22E-59	-1.69E-46
MS.gene060830	MS.gene20681	0.830119	3.51E-55	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene20681	MS.gene02213	PPI
MS.gene20681	MS.gene047206	PPI
MS.gene20681	MS.gene20043	PPI
MS.gene20681	MS.gene88635	PPI
MS.gene20681	MS.gene36329	PPI
MS.gene20681	MS.gene48368	PPI
MS.gene20681	MS.gene09988	PPI
MS.gene20681	MS.gene32091	PPI
MS.gene20681	MS.gene007453	PPI
MS.gene20681	MS.gene33304	PPI
MS.gene20681	MS.gene040439	PPI
MS.gene20681	MS.gene50744	PPI
MS.gene20681	MS.gene32092	PPI
MS.gene20681	MS.gene072782	PPI
MS.gene20681	MS.gene03126	PPI
MS.gene20681	MS.gene063385	PPI
MS.gene20681	MS.gene002256	PPI
MS.gene20681	MS.gene47800	PPI
MS.gene20681	MS.gene27648	PPI
MS.gene20681	MS.gene00612	PPI
MS.gene20681	MS.gene66284	PPI
MS.gene20681	MS.gene40020	PPI
MS.gene20681	MS.gene52775	PPI
MS.gene20681	MS.gene35471	PPI
MS.gene20681	MS.gene005792	PPI
MS.gene20681	MS.gene003290	PPI
MS.gene20681	MS.gene68085	PPI
MS.gene20681	MS.gene014351	PPI
MS.gene20681	MS.gene023799	PPI
MS.gene20681	MS.gene067210	PPI
MS.gene20681	MS.gene52173	PPI
MS.gene20681	MS.gene72768	PPI
MS.gene20681	MS.gene95472	PPI
MS.gene20681	MS.gene040601	PPI
MS.gene20681	MS.gene95471	PPI
MS.gene20681	MS.gene76511	PPI
MS.gene20681	MS.gene94588	PPI
MS.gene20681	MS.gene37310	PPI
MS.gene79484	MS.gene20681	PPI
MS.gene20681	MS.gene20045	PPI
MS.gene20681	MS.gene004704	PPI
MS.gene20681	MS.gene42236	PPI
MS.gene20681	MS.gene060830	PPI
MS.gene20681	MS.gene91151	PPI
MS.gene20681	MS.gene75111	PPI
MS.gene20681	MS.gene69331	PPI
MS.gene20681	MS.gene29448	PPI
MS.gene20681	MS.gene003693	PPI
MS.gene20681	MS.gene60517	PPI
MS.gene20681	MS.gene23314	PPI
MS.gene20681	MS.gene40019	PPI
MS.gene20681	MS.gene02090	PPI
MS.gene20681	MS.gene56868	PPI
MS.gene20681	MS.gene53798	PPI
MS.gene20681	MS.gene82426	PPI
MS.gene20681	MS.gene034044	PPI
MS.gene20681	MS.gene01229	PPI
MS.gene20681	MS.gene022438	PPI
MS.gene20681	MS.gene064831	PPI
MS.gene20681	MS.gene034046	PPI
MS.gene20681	MS.gene89206	PPI
MS.gene20681	MS.gene007492	PPI
MS.gene20681	MS.gene073150	PPI
MS.gene20681	MS.gene019309	PPI
MS.gene20681	MS.gene85127	PPI
MS.gene20681	MS.gene004705	PPI
MS.gene20681	MS.gene033253	PPI
MS.gene20681	MS.gene039132	PPI
MS.gene20681	MS.gene042172	PPI
MS.gene20681	MS.gene030258	PPI
MS.gene20681	MS.gene012655	PPI
MS.gene20681	MS.gene68942	PPI
MS.gene20681	MS.gene026156	PPI
MS.gene20681	MS.gene74552	PPI
MS.gene20681	MS.gene77667	PPI
MS.gene20681	MS.gene072064	PPI
MS.gene20681	MS.gene26077	PPI
MS.gene20681	MS.gene91244	PPI
MS.gene20681	MS.gene028153	PPI
MS.gene20681	MS.gene002038	PPI
MS.gene20681	MS.gene83444	PPI
MS.gene20681	MS.gene22456	PPI
MS.gene20681	MS.gene77076	PPI
MS.gene20681	MS.gene98278	PPI
MS.gene20681	MS.gene026793	PPI
MS.gene20681	MS.gene017434	PPI
MS.gene20681	MS.gene015586	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20681.t1	MTR_1g089260	97.358	530	14	0	1	530	1	530	0.0	1072
MS.gene20681.t1	MTR_1g072140	30.994	171	113	5	80	249	22	188	7.68e-16	79.0
MS.gene20681.t1	MTR_4g125710	30.270	185	113	4	57	228	117	298	4.86e-15	76.6
MS.gene20681.t1	MTR_2g012630	23.830	235	163	7	98	330	29	249	1.78e-12	70.9
MS.gene20681.t1	MTR_2g012630	23.830	235	163	7	98	330	29	249	2.16e-12	70.5
MS.gene20681.t1	MTR_4g119620	29.500	200	125	8	74	263	18	211	2.40e-12	68.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20681.t1	AT2G47990	58.757	531	207	6	1	529	9	529	0.0	603
MS.gene20681.t1	AT4G29830	32.653	147	96	2	88	234	161	304	8.48e-14	72.8
MS.gene20681.t1	AT5G50230	27.027	185	117	6	138	319	277	446	1.49e-12	70.5
MS.gene20681.t1	AT3G49660	28.415	183	120	7	77	253	16	193	2.01e-12	68.9
MS.gene20681.t1	AT4G02730	26.061	165	116	6	80	242	38	198	6.30e-12	67.4
MS.gene20681.t1	AT5G13480	24.051	316	206	12	46	356	108	394	4.98e-11	65.9
MS.gene20681.t1	AT3G15980	24.904	261	180	10	75	331	5	253	5.79e-11	65.9
MS.gene20681.t1	AT5G13480	24.051	316	206	12	46	356	85	371	5.90e-11	65.5
MS.gene20681.t1	AT3G15980	24.904	261	180	10	75	331	5	253	6.38e-11	65.9
MS.gene20681.t1	AT3G15980	24.904	261	180	10	75	331	5	253	6.42e-11	65.9
MS.gene20681.t1	AT3G15980	24.904	261	180	10	75	331	5	253	6.91e-11	65.5
MS.gene20681.t1	AT3G15980	24.904	261	180	10	75	331	5	253	6.91e-11	65.5
MS.gene20681.t1	AT3G15980	24.904	261	180	10	75	331	5	253	6.91e-11	65.5
MS.gene20681.t1	AT3G15980	24.904	261	180	10	75	331	5	253	6.91e-11	65.5
MS.gene20681.t1	AT5G13480	24.051	316	206	12	46	356	85	371	7.17e-11	65.5
MS.gene20681.t1	AT5G13480	24.051	316	206	12	46	356	97	383	8.31e-11	65.1

Find 173 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAGGTTTGGGATTGTGTTCT+TGG	0.157342	1.2:+66898992	None:intergenic
GATAAGTTGCATTTAGTTTC+TGG	0.212466	1.2:-66898635	MS.gene20681:CDS
GCGAAGGAAAAGGGTGGATT+TGG	0.214434	1.2:+66898893	None:intergenic
GCAGTCAACTTCACCTTCTT+TGG	0.223900	1.2:+66897873	None:intergenic
CAAGTATTCTAGTTTGTTAA+TGG	0.227969	1.2:-66897655	MS.gene20681:CDS
GAAGGGATGGAGAATTGTTT+AGG	0.237807	1.2:+66898929	None:intergenic
TTTGAAGGAAGACCAGTATT+TGG	0.239917	1.2:+66898964	None:intergenic
TTGTTAAGGTTTGGGATGTT+AGG	0.254425	1.2:-66898469	MS.gene20681:CDS
GGAGAAGCTGGAACTGCTTT+TGG	0.259476	1.2:-66897706	MS.gene20681:CDS
GTTAATGGGATTGGCTAATA+AGG	0.271721	1.2:-66897640	MS.gene20681:CDS
GGGAGGATCGGGAAGGATTC+GGG	0.272953	1.2:-66898254	MS.gene20681:CDS
CTAGTTCGGTTTGGAGAATT+AGG	0.279451	1.2:-66898001	MS.gene20681:CDS
GTGGGAATGCGGTCAAGATT+TGG	0.293697	1.2:-66898349	MS.gene20681:CDS
GTTTGGGATGTTAGGGTTAG+AGG	0.297753	1.2:-66898461	MS.gene20681:CDS
AAGGGATGGAGAATTGTTTA+GGG	0.316527	1.2:+66898930	None:intergenic
GTATTTGGATTCAGAGGTTT+GGG	0.323308	1.2:+66898979	None:intergenic
AAATGAGGGCTGATGATATT+AGG	0.325566	1.2:-66897611	MS.gene20681:CDS
CATTAACAAACTAGAATACT+TGG	0.351333	1.2:+66897656	None:intergenic
GATCAAACAATCGTCCTGTT+AGG	0.358606	1.2:-66898679	MS.gene20681:CDS
TGGGAGGATCGGGAAGGATT+CGG	0.360564	1.2:-66898255	MS.gene20681:CDS
TCTCCTTTGTTGAGAATTGC+TGG	0.363442	1.2:-66897489	MS.gene20681:CDS
CGAAGGAAAAGGGTGGATTT+GGG	0.363489	1.2:+66898894	None:intergenic
AAGGTGGATGAGAAGGAAGT+TGG	0.363701	1.2:-66898035	MS.gene20681:CDS
TGGGAATGCGGTCAAGATTT+GGG	0.370097	1.2:-66898348	MS.gene20681:CDS
AAGTATTCTAGTTTGTTAAT+GGG	0.370766	1.2:-66897654	MS.gene20681:CDS
CACATCTAACATAATCCTTA+TGG	0.370873	1.2:+66898546	None:intergenic
AGTATTTGGATTCAGAGGTT+TGG	0.376412	1.2:+66898978	None:intergenic
TGATTTAGATTTGGAGAAGC+TGG	0.378998	1.2:-66897718	MS.gene20681:CDS
GTTGTTTATTTGCCGGGTGG+TGG	0.385067	1.2:-66898389	MS.gene20681:CDS
TTCTAGTTTGTTAATGGGAT+TGG	0.396417	1.2:-66897649	MS.gene20681:CDS
AGAATTGTTTAGGGATTTGT+TGG	0.400534	1.2:+66898939	None:intergenic
GGAGGATGTTGTTTATTTGC+CGG	0.402345	1.2:-66898396	MS.gene20681:CDS
TGTTAAGGTTTGGGATGTTA+GGG	0.406978	1.2:-66898468	MS.gene20681:CDS
TTGGGATTGTGTTCTTGGTT+TGG	0.407303	1.2:+66898997	None:intergenic
ATTCGCCGGATTGTTCTACT+AGG	0.408446	1.2:-66898106	MS.gene20681:CDS
ACCATCTGAGGGTGATTACT+TGG	0.408850	1.2:-66897904	MS.gene20681:CDS
ACAAAACAATCAGAACTAAC+CGG	0.413789	1.2:+66898512	None:intergenic
AGGTGGATGAGAAGGAAGTT+GGG	0.415544	1.2:-66898034	MS.gene20681:CDS
GATTGTTCTACTAGGGTGAT+TGG	0.420300	1.2:-66898098	MS.gene20681:CDS
AGATTTGTGTTGGGAGGATC+GGG	0.425705	1.2:-66898265	MS.gene20681:CDS
TGATGCCGTTGTTAGGTATT+GGG	0.427423	1.2:-66898606	MS.gene20681:CDS
GTGTGTTTCTGATTTAGATT+TGG	0.429034	1.2:-66897727	MS.gene20681:CDS
TAGGATTATGAGTGTTGGTT+TGG	0.431430	1.2:-66898213	MS.gene20681:CDS
AAGATTTGGGATTTGATTGG+TGG	0.437801	1.2:-66898335	MS.gene20681:CDS
GGATAATGAACAAACCTAAC+AGG	0.439146	1.2:+66898665	None:intergenic
GGTTGCTGTGATGGAGGAAT+TGG	0.439980	1.2:-66897772	MS.gene20681:CDS
TGGGGTGAGTGAGGCTAGTT+CGG	0.440336	1.2:-66898015	MS.gene20681:CDS
AGGAGGAGATGGTGGCGGTT+TGG	0.451413	1.2:+66898822	None:intergenic
GGTGGGATGATTGCAACAGC+TGG	0.452218	1.2:-66898371	MS.gene20681:CDS
ATGATGCCGTTGTTAGGTAT+TGG	0.452405	1.2:-66898607	MS.gene20681:CDS
AAAGGGTGGATTTGGGGAGA+AGG	0.453317	1.2:+66898901	None:intergenic
ATTAGCGGCGGCGAAGGAAA+AGG	0.456551	1.2:+66898883	None:intergenic
CATGGTGTTTCGTGGCCATA+AGG	0.457919	1.2:-66898561	MS.gene20681:CDS
GGGATTTGATTGGTGGTGGG+AGG	0.459347	1.2:-66898328	MS.gene20681:CDS
ATTTGGGATTTGATTGGTGG+TGG	0.460343	1.2:-66898332	MS.gene20681:CDS
AAGATTTGTGTTGGGAGGAT+CGG	0.463451	1.2:-66898266	MS.gene20681:CDS
CTAATAAGGTTATTGAAATG+AGG	0.469043	1.2:-66897626	MS.gene20681:CDS
GTCAAGATTTGGGATTTGAT+TGG	0.475359	1.2:-66898338	MS.gene20681:CDS
CTCGCCGCTTCTGACCTCTC+CGG	0.476338	1.2:-66898752	MS.gene20681:CDS
GAATTGTTTAGGGATTTGTT+GGG	0.479821	1.2:+66898940	None:intergenic
TGAGTGAGGCTAGTTCGGTT+TGG	0.481655	1.2:-66898010	MS.gene20681:CDS
AGGGCATGTGGTTGCTGTGA+TGG	0.482493	1.2:-66897781	MS.gene20681:CDS
TGATCTAAGACGACGGAGAC+GGG	0.486170	1.2:+66898697	None:intergenic
TCGCCGCTTCTGACCTCTCC+GGG	0.487189	1.2:-66898751	MS.gene20681:CDS
TGGATTTGGGGAGAAGGTGA+TGG	0.489356	1.2:+66898907	None:intergenic
CTTGGTTATGAAGCCAAAGA+AGG	0.489834	1.2:-66897886	MS.gene20681:CDS
GAAGGTAAAGGTGGATGAGA+AGG	0.491134	1.2:-66898042	MS.gene20681:CDS
GTGTTGGAAGGTAAGAATCC+AGG	0.495016	1.2:-66897801	MS.gene20681:CDS
AAGAGTTTGAGAGCTGAAGA+TGG	0.496356	1.2:+66898847	None:intergenic
AAACCTCTGAATCCAAATAC+TGG	0.498293	1.2:-66898976	MS.gene20681:CDS
CGGTGACGAAGATTTGTGTT+GGG	0.498679	1.2:-66898274	MS.gene20681:CDS
CAGTATAGGATTATGAGTGT+TGG	0.498953	1.2:-66898218	MS.gene20681:CDS
GTTTGATTACGGTAATATGA+AGG	0.499792	1.2:-66898174	MS.gene20681:CDS
AGCTGAATTAGCGGCGGCGA+AGG	0.501815	1.2:+66898877	None:intergenic
CAGTCGTTGCAAGAGATACA+AGG	0.510806	1.2:-66897519	MS.gene20681:CDS
GGAGGAGACGGTGTCGGAGA+AGG	0.512976	1.2:+66898802	None:intergenic
TTTGGGGAGAAGGTGATGGA+AGG	0.518211	1.2:+66898911	None:intergenic
TTGTTTATTTGCCGGGTGGT+GGG	0.518288	1.2:-66898388	MS.gene20681:CDS
ATTATGAGTGTTGGTTTGGA+TGG	0.518342	1.2:-66898209	MS.gene20681:CDS
GGAGGATCGGGAAGGATTCG+GGG	0.520732	1.2:-66898253	MS.gene20681:CDS
CAAAACAATCAGAACTAACC+GGG	0.520896	1.2:+66898513	None:intergenic
GTAGTGATGATGCCGTTGTT+AGG	0.523290	1.2:-66898613	MS.gene20681:CDS
CGAGGAGACGAGCATCGTTA+CGG	0.525818	1.2:+66898771	None:intergenic
TTTGAGAGCTGAAGATGGAG+AGG	0.526433	1.2:+66898852	None:intergenic
ATCACAGCAACCACATGCCC+TGG	0.526670	1.2:+66897783	None:intergenic
GGAATGAGTCGGATCAGTAT+AGG	0.527668	1.2:-66898232	MS.gene20681:CDS
AGGGTGATTGGAACTTCGAA+TGG	0.528923	1.2:-66898086	MS.gene20681:CDS
GAAGGAAGTTGGGGTGAGTG+AGG	0.529605	1.2:-66898024	MS.gene20681:CDS
TTGATCTAAGACGACGGAGA+CGG	0.530178	1.2:+66898696	None:intergenic
TTGGAGAATTAGGCCGGTGG+AGG	0.532273	1.2:-66897991	MS.gene20681:CDS
GCACTGTCGAGACAGAGATT+CGG	0.536660	1.2:-66897548	MS.gene20681:CDS
GAGGATGTTGTTTATTTGCC+GGG	0.537889	1.2:-66898395	MS.gene20681:CDS
TTTGGGATTTGATTGGTGGT+GGG	0.542819	1.2:-66898331	MS.gene20681:CDS
ACTCCGGTCATGGTGTTTCG+TGG	0.543833	1.2:-66898569	MS.gene20681:CDS
TTCGGTTTGGAGAATTAGGC+CGG	0.544980	1.2:-66897997	MS.gene20681:CDS
AGGTGAGACCAACTCGGTGA+TGG	0.549474	1.2:-66898441	MS.gene20681:CDS
GGAGACGGTGTCGGAGAAGG+AGG	0.550158	1.2:+66898805	None:intergenic
CCAACAGACAAAAGCGGCGC+AGG	0.551615	1.2:+66898131	None:intergenic
AGACCAGTATTTGGATTCAG+AGG	0.552256	1.2:+66898973	None:intergenic
TTTGTCTGTTGGGTATTCGC+CGG	0.553778	1.2:-66898120	MS.gene20681:CDS
CGTCTTATTATGACTCTCCA+TGG	0.554512	1.2:+66898295	None:intergenic
TTAGCGGCGGCGAAGGAAAA+GGG	0.555254	1.2:+66898884	None:intergenic
TTCTTCTCAGTATCCTCCAC+CGG	0.556705	1.2:+66897978	None:intergenic
GGAGAAGGTGATGGAAGGGA+TGG	0.558858	1.2:+66898916	None:intergenic
TAATAAGGTTATTGAAATGA+GGG	0.559117	1.2:-66897625	MS.gene20681:CDS
TACTGAGGAAACTCCGGTCA+TGG	0.559282	1.2:-66898579	MS.gene20681:CDS
TGGCCACGAAACACCATGAC+CGG	0.559409	1.2:+66898566	None:intergenic
ATTAAAATCCATCACCGAGT+TGG	0.559727	1.2:+66898433	None:intergenic
TTGAGAGCTGAAGATGGAGA+GGG	0.559770	1.2:+66898853	None:intergenic
TGATGGAGGAATTGGTGTCT+AGG	0.560038	1.2:-66897764	MS.gene20681:CDS
ATCGAAGATTTGAATTAACC+CGG	0.570756	1.2:+66898733	None:intergenic
TTGGGGAGAAGGTGATGGAA+GGG	0.571747	1.2:+66898912	None:intergenic
GGGTGATTGGAACTTCGAAT+GGG	0.571974	1.2:-66898085	MS.gene20681:CDS
CTTCCAGCAATTCTCAACAA+AGG	0.572053	1.2:+66897486	None:intergenic
GGTGGAGGATACTGAGAAGA+AGG	0.572161	1.2:-66897976	MS.gene20681:CDS
ACGGTGACGAAGATTTGTGT+TGG	0.574806	1.2:-66898275	MS.gene20681:CDS
GGGATGATTGCAACAGCTGG+TGG	0.577309	1.2:-66898368	MS.gene20681:CDS
TGGGAGGCTTGTGTATTCCA+TGG	0.578149	1.2:-66898312	MS.gene20681:CDS
GTCGGAGAAGGAGGAGATGG+TGG	0.579404	1.2:+66898814	None:intergenic
GGAGAGGGAAGCTGAATTAG+CGG	0.586229	1.2:+66898868	None:intergenic
TAGATGTGGTGATTCGTCCC+CGG	0.586555	1.2:-66898531	MS.gene20681:CDS
GAATAAGTTTAGGCATGGTG+AGG	0.586991	1.2:-66897835	MS.gene20681:CDS
GGTGTCGGAGAAGGAGGAGA+TGG	0.587195	1.2:+66898811	None:intergenic
GTTGCAATCATCCCACCACC+CGG	0.587460	1.2:+66898377	None:intergenic
TTGTGTTGGGAGGATCGGGA+AGG	0.588502	1.2:-66898261	MS.gene20681:CDS
GAAGGATTCGGGGAATGAGT+CGG	0.589250	1.2:-66898243	MS.gene20681:CDS
GGTGGATGAGAAGGAAGTTG+GGG	0.590324	1.2:-66898033	MS.gene20681:CDS
TAGGTATTGGGATGTTACTG+AGG	0.593479	1.2:-66898594	MS.gene20681:CDS
GCTCTTGTGTGCGTGTTGGA+AGG	0.593734	1.2:-66897813	MS.gene20681:CDS
ATCACCCTAGTAGAACAATC+CGG	0.594271	1.2:+66898101	None:intergenic
TGTTGGAAGGTAAGAATCCA+GGG	0.600042	1.2:-66897800	MS.gene20681:CDS
TAATCATGGTTGTCCTGTGG+AGG	0.600616	1.2:-66898414	MS.gene20681:CDS
TTCGCCGGATTGTTCTACTA+GGG	0.603643	1.2:-66898105	MS.gene20681:CDS
TGAGGCTCTTGTGTGCGTGT+TGG	0.603731	1.2:-66897817	MS.gene20681:CDS
GAATACCCAACAGACAAAAG+CGG	0.604064	1.2:+66898125	None:intergenic
TGCTGCAAAGAGGAAGGTAA+AGG	0.605968	1.2:-66898054	MS.gene20681:CDS
AATTTATGCTGCAAAGAGGA+AGG	0.608646	1.2:-66898060	MS.gene20681:CDS
ATTAACAAACTAGAATACTT+GGG	0.616436	1.2:+66897657	None:intergenic
AAATAAACAACATCCTCCAC+AGG	0.617238	1.2:+66898401	None:intergenic
CGGAGAGGTCAGAAGCGGCG+AGG	0.618345	1.2:+66898753	None:intergenic
ACCAAGTAATCACCCTCAGA+TGG	0.618575	1.2:+66897903	None:intergenic
GGATGATTGCAACAGCTGGT+GGG	0.618810	1.2:-66898367	MS.gene20681:CDS
AGGTAAGAATCCAGGGCATG+TGG	0.620054	1.2:-66897793	MS.gene20681:CDS
AGAGACGGAGGAGACGGTGT+CGG	0.621983	1.2:+66898796	None:intergenic
GGAGAAGGAGGAGATGGTGG+CGG	0.624254	1.2:+66898817	None:intergenic
GAAGGAAAAGGGTGGATTTG+GGG	0.624822	1.2:+66898895	None:intergenic
GGATGTTACTGAGGAAACTC+CGG	0.626392	1.2:-66898585	MS.gene20681:CDS
ACGGAAAGAGACGGAGGAGA+CGG	0.629393	1.2:+66898790	None:intergenic
CATGGAGAGTCATAATAAGA+CGG	0.634586	1.2:-66898294	MS.gene20681:CDS
AGATTTGAATTAACCCGGAG+AGG	0.635639	1.2:+66898738	None:intergenic
CAAGGTGAGAAACCATCTGA+GGG	0.636287	1.2:-66897915	MS.gene20681:CDS
TGACGAAGATTTGTGTTGGG+AGG	0.639147	1.2:-66898271	MS.gene20681:CDS
GCATGTGGTTGCTGTGATGG+AGG	0.640066	1.2:-66897778	MS.gene20681:CDS
GCAAGGTGAGAAACCATCTG+AGG	0.641081	1.2:-66897916	MS.gene20681:CDS
GGATAATTTATGCTGCAAAG+AGG	0.645362	1.2:-66898064	MS.gene20681:CDS
TGCAACAGCTGGTGGGAATG+CGG	0.649987	1.2:-66898360	MS.gene20681:CDS
GGTTAGAGGTGAGACCAACT+CGG	0.655570	1.2:-66898447	MS.gene20681:CDS
TGAAGGTTACTTATTCGATG+AGG	0.658542	1.2:-66898157	MS.gene20681:CDS
GATTGTTTGATCTAAGACGA+CGG	0.662044	1.2:+66898690	None:intergenic
GGTTTGGAGAATTAGGCCGG+TGG	0.670090	1.2:-66897994	MS.gene20681:CDS
AAATGCAACTTATCATGACG+CGG	0.673001	1.2:+66898644	None:intergenic
AGCATCGTTACGGAAAGAGA+CGG	0.674964	1.2:+66898781	None:intergenic
GATGTTGTTTATTTGCCGGG+TGG	0.677452	1.2:-66898392	MS.gene20681:CDS
GAGGGAAGCTGAATTAGCGG+CGG	0.678014	1.2:+66898871	None:intergenic
CATAAGGATTATGTTAGATG+TGG	0.678530	1.2:-66898545	MS.gene20681:CDS
GCGGCGGCGAAGGAAAAGGG+TGG	0.680220	1.2:+66898887	None:intergenic
TGCAAAGAGGAAGGTAAAGG+TGG	0.681900	1.2:-66898051	MS.gene20681:CDS
AGTCGTTGCAAGAGATACAA+GGG	0.683300	1.2:-66897518	MS.gene20681:CDS
ATCGTTACGGAAAGAGACGG+AGG	0.689504	1.2:+66898784	None:intergenic
TAACCCGGAGAGGTCAGAAG+CGG	0.691165	1.2:+66898748	None:intergenic
AACATCCCAATACCTAACAA+CGG	0.692068	1.2:+66898601	None:intergenic
TGTCTAGGAAGAAGTTGTTG+AGG	0.703968	1.2:-66897749	MS.gene20681:CDS
AAAACAATCAGAACTAACCG+GGG	0.725083	1.2:+66898514	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTAACAAACTAGAATACTT+GGG	+	chr1.2:66898874-66898893	None:intergenic	20.0%
!!	TAATAAGGTTATTGAAATGA+GGG	-	chr1.2:66898903-66898922	MS.gene20681:CDS	20.0%
!!!	AAGTATTCTAGTTTGTTAAT+GGG	-	chr1.2:66898874-66898893	MS.gene20681:CDS	20.0%
!!!	ATAAGCTTTTGAATAAGTTT+AGG	-	chr1.2:66898683-66898702	MS.gene20681:CDS	20.0%
!!!	TATTTTAAGGTGTTTGATTA+CGG	-	chr1.2:66898343-66898362	MS.gene20681:CDS	20.0%
!!!	TTTTGAAAACTACTAACTTT+AGG	-	chr1.2:66898575-66898594	MS.gene20681:CDS	20.0%
!	CATTAACAAACTAGAATACT+TGG	+	chr1.2:66898875-66898894	None:intergenic	25.0%
!	CTAATAAGGTTATTGAAATG+AGG	-	chr1.2:66898902-66898921	MS.gene20681:CDS	25.0%
!!	AACTTTAGGTATTTTCAAAG+AGG	-	chr1.2:66898589-66898608	MS.gene20681:CDS	25.0%
!!	ACTTTAGGTATTTTCAAAGA+GGG	-	chr1.2:66898590-66898609	MS.gene20681:CDS	25.0%
!!	ATGATCATTTTGTTAAGGTT+TGG	-	chr1.2:66898050-66898069	MS.gene20681:CDS	25.0%
!!	CTCTTATGATCATTTTGTTA+AGG	-	chr1.2:66898045-66898064	MS.gene20681:CDS	25.0%
!!	TGATCATTTTGTTAAGGTTT+GGG	-	chr1.2:66898051-66898070	MS.gene20681:CDS	25.0%
!!!	CAAGTATTCTAGTTTGTTAA+TGG	-	chr1.2:66898873-66898892	MS.gene20681:CDS	25.0%
!!!	TTTTGAGTTTAGGTTTAACT+GGG	+	chr1.2:66897511-66897530	None:intergenic	25.0%
	ACAAAACAATCAGAACTAAC+CGG	+	chr1.2:66898019-66898038	None:intergenic	30.0%
	AGAATTGTTTAGGGATTTGT+TGG	+	chr1.2:66897592-66897611	None:intergenic	30.0%
	ATCGAAGATTTGAATTAACC+CGG	+	chr1.2:66897798-66897817	None:intergenic	30.0%
	CACATCTAACATAATCCTTA+TGG	+	chr1.2:66897985-66898004	None:intergenic	30.0%
	CATAAGGATTATGTTAGATG+TGG	-	chr1.2:66897983-66898002	MS.gene20681:CDS	30.0%
	GTTTGATTACGGTAATATGA+AGG	-	chr1.2:66898354-66898373	MS.gene20681:CDS	30.0%
!	CTTTTGAATAAGTTTAGGCA+TGG	-	chr1.2:66898688-66898707	MS.gene20681:CDS	30.0%
!	GAATTGTTTAGGGATTTGTT+GGG	+	chr1.2:66897591-66897610	None:intergenic	30.0%
!	GATAAGTTGCATTTAGTTTC+TGG	-	chr1.2:66897893-66897912	MS.gene20681:CDS	30.0%
!	GTGTGTTTCTGATTTAGATT+TGG	-	chr1.2:66898801-66898820	MS.gene20681:CDS	30.0%
!!	AGTTCTGATTGTTTTGTTAC+TGG	-	chr1.2:66898022-66898041	MS.gene20681:CDS	30.0%
!!	TGGTTTGGATGGTTATTTTA+AGG	-	chr1.2:66898330-66898349	MS.gene20681:CDS	30.0%
!!	TTCTAGTTTGTTAATGGGAT+TGG	-	chr1.2:66898879-66898898	MS.gene20681:CDS	30.0%
!!!	GTTTTGAGTTTAGGTTTAAC+TGG	+	chr1.2:66897512-66897531	None:intergenic	30.0%
	AAAACAATCAGAACTAACCG+GGG	+	chr1.2:66898017-66898036	None:intergenic	35.0%
	AAACCTCTGAATCCAAATAC+TGG	-	chr1.2:66897552-66897571	MS.gene20681:CDS	35.0%
	AAATAAACAACATCCTCCAC+AGG	+	chr1.2:66898130-66898149	None:intergenic	35.0%
	AAATGAGGGCTGATGATATT+AGG	-	chr1.2:66898917-66898936	MS.gene20681:CDS	35.0%
	AAATGCAACTTATCATGACG+CGG	+	chr1.2:66897887-66897906	None:intergenic	35.0%
	AACATCCCAATACCTAACAA+CGG	+	chr1.2:66897930-66897949	None:intergenic	35.0%
	AAGGGATGGAGAATTGTTTA+GGG	+	chr1.2:66897601-66897620	None:intergenic	35.0%
	AATTTATGCTGCAAAGAGGA+AGG	-	chr1.2:66898468-66898487	MS.gene20681:CDS	35.0%
	AGTATTTGGATTCAGAGGTT+TGG	+	chr1.2:66897553-66897572	None:intergenic	35.0%
	ATTAAAATCCATCACCGAGT+TGG	+	chr1.2:66898098-66898117	None:intergenic	35.0%
	CAAAACAATCAGAACTAACC+GGG	+	chr1.2:66898018-66898037	None:intergenic	35.0%
	CAGTATAGGATTATGAGTGT+TGG	-	chr1.2:66898310-66898329	MS.gene20681:CDS	35.0%
	CATGGAGAGTCATAATAAGA+CGG	-	chr1.2:66898234-66898253	MS.gene20681:CDS	35.0%
	GGATAATGAACAAACCTAAC+AGG	+	chr1.2:66897866-66897885	None:intergenic	35.0%
	GGATAATTTATGCTGCAAAG+AGG	-	chr1.2:66898464-66898483	MS.gene20681:CDS	35.0%
	GTATTTGGATTCAGAGGTTT+GGG	+	chr1.2:66897552-66897571	None:intergenic	35.0%
	TGAAGGTTACTTATTCGATG+AGG	-	chr1.2:66898371-66898390	MS.gene20681:CDS	35.0%
	TGATTTAGATTTGGAGAAGC+TGG	-	chr1.2:66898810-66898829	MS.gene20681:CDS	35.0%
	TTTGAAGGAAGACCAGTATT+TGG	+	chr1.2:66897567-66897586	None:intergenic	35.0%
!	AAGATTTGGGATTTGATTGG+TGG	-	chr1.2:66898193-66898212	MS.gene20681:CDS	35.0%
!	GATTGTTTGATCTAAGACGA+CGG	+	chr1.2:66897841-66897860	None:intergenic	35.0%
!	GTCAAGATTTGGGATTTGAT+TGG	-	chr1.2:66898190-66898209	MS.gene20681:CDS	35.0%
!	TGTTAAGGTTTGGGATGTTA+GGG	-	chr1.2:66898060-66898079	MS.gene20681:CDS	35.0%
!	TTGTTAAGGTTTGGGATGTT+AGG	-	chr1.2:66898059-66898078	MS.gene20681:CDS	35.0%
!	TTTTAATCATGGTTGTCCTG+TGG	-	chr1.2:66898111-66898130	MS.gene20681:CDS	35.0%
!!	ATTATGAGTGTTGGTTTGGA+TGG	-	chr1.2:66898319-66898338	MS.gene20681:CDS	35.0%
!!	GTTAATGGGATTGGCTAATA+AGG	-	chr1.2:66898888-66898907	MS.gene20681:CDS	35.0%
!!	TAGGATTATGAGTGTTGGTT+TGG	-	chr1.2:66898315-66898334	MS.gene20681:CDS	35.0%
!!!	CTTGGTTTGGTTTTGAGTTT+AGG	+	chr1.2:66897521-66897540	None:intergenic	35.0%
!!!	TCGGTGATGGATTTTAATCA+TGG	-	chr1.2:66898100-66898119	MS.gene20681:CDS	35.0%
	AAGAGTTTGAGAGCTGAAGA+TGG	+	chr1.2:66897684-66897703	None:intergenic	40.0%
	AAGATTTGTGTTGGGAGGAT+CGG	-	chr1.2:66898262-66898281	MS.gene20681:CDS	40.0%
	AGACCAGTATTTGGATTCAG+AGG	+	chr1.2:66897558-66897577	None:intergenic	40.0%
	AGATTTGAATTAACCCGGAG+AGG	+	chr1.2:66897793-66897812	None:intergenic	40.0%
	AGTCGTTGCAAGAGATACAA+GGG	-	chr1.2:66899010-66899029	MS.gene20681:CDS	40.0%
	ATCACCCTAGTAGAACAATC+CGG	+	chr1.2:66898430-66898449	None:intergenic	40.0%
	ATGATGCCGTTGTTAGGTAT+TGG	-	chr1.2:66897921-66897940	MS.gene20681:CDS	40.0%
	CTTCCAGCAATTCTCAACAA+AGG	+	chr1.2:66899045-66899064	None:intergenic	40.0%
	CTTGGTTATGAAGCCAAAGA+AGG	-	chr1.2:66898642-66898661	MS.gene20681:CDS	40.0%
	GAAGGGATGGAGAATTGTTT+AGG	+	chr1.2:66897602-66897621	None:intergenic	40.0%
	GAATAAGTTTAGGCATGGTG+AGG	-	chr1.2:66898693-66898712	MS.gene20681:CDS	40.0%
	GAATACCCAACAGACAAAAG+CGG	+	chr1.2:66898406-66898425	None:intergenic	40.0%
	GATCAAACAATCGTCCTGTT+AGG	-	chr1.2:66897849-66897868	MS.gene20681:CDS	40.0%
	GATTGTTCTACTAGGGTGAT+TGG	-	chr1.2:66898430-66898449	MS.gene20681:CDS	40.0%
	TCTCCTTTGTTGAGAATTGC+TGG	-	chr1.2:66899039-66899058	MS.gene20681:CDS	40.0%
	TGATGCCGTTGTTAGGTATT+GGG	-	chr1.2:66897922-66897941	MS.gene20681:CDS	40.0%
	TGTCTAGGAAGAAGTTGTTG+AGG	-	chr1.2:66898779-66898798	MS.gene20681:CDS	40.0%
	TGTTGGAAGGTAAGAATCCA+GGG	-	chr1.2:66898728-66898747	MS.gene20681:CDS	40.0%
	TTGGGATTGTGTTCTTGGTT+TGG	+	chr1.2:66897534-66897553	None:intergenic	40.0%
!	AGGTATTTTCAAAGAGGGCA+AGG	-	chr1.2:66898595-66898614	MS.gene20681:CDS	40.0%
!	ATTTGGGATTTGATTGGTGG+TGG	-	chr1.2:66898196-66898215	MS.gene20681:CDS	40.0%
!	CGTCTTATTATGACTCTCCA+TGG	+	chr1.2:66898236-66898255	None:intergenic	40.0%
!	GAGGATGTTGTTTATTTGCC+GGG	-	chr1.2:66898133-66898152	MS.gene20681:CDS	40.0%
!	GGAGGATGTTGTTTATTTGC+CGG	-	chr1.2:66898132-66898151	MS.gene20681:CDS	40.0%
!	TTTGGGATTTGATTGGTGGT+GGG	-	chr1.2:66898197-66898216	MS.gene20681:CDS	40.0%
!!	CTAGTTCGGTTTGGAGAATT+AGG	-	chr1.2:66898527-66898546	MS.gene20681:CDS	40.0%
!!	TAGGTATTGGGATGTTACTG+AGG	-	chr1.2:66897934-66897953	MS.gene20681:CDS	40.0%
!!!	AGGGATTTGTTGGGTTTTGA+AGG	+	chr1.2:66897582-66897601	None:intergenic	40.0%
	AAGGTGGATGAGAAGGAAGT+TGG	-	chr1.2:66898493-66898512	MS.gene20681:CDS	45.0%
	ACCAAGTAATCACCCTCAGA+TGG	+	chr1.2:66898628-66898647	None:intergenic	45.0%
	ACCATCTGAGGGTGATTACT+TGG	-	chr1.2:66898624-66898643	MS.gene20681:CDS	45.0%
	ACGGTGACGAAGATTTGTGT+TGG	-	chr1.2:66898253-66898272	MS.gene20681:CDS	45.0%
	AGATTTGTGTTGGGAGGATC+GGG	-	chr1.2:66898263-66898282	MS.gene20681:CDS	45.0%
	AGCATCGTTACGGAAAGAGA+CGG	+	chr1.2:66897750-66897769	None:intergenic	45.0%
	AGGGTGATTGGAACTTCGAA+TGG	-	chr1.2:66898442-66898461	MS.gene20681:CDS	45.0%
	AGGTGGATGAGAAGGAAGTT+GGG	-	chr1.2:66898494-66898513	MS.gene20681:CDS	45.0%
	ATTCGCCGGATTGTTCTACT+AGG	-	chr1.2:66898422-66898441	MS.gene20681:CDS	45.0%
	CAAGGTGAGAAACCATCTGA+GGG	-	chr1.2:66898613-66898632	MS.gene20681:CDS	45.0%
	CAGTCGTTGCAAGAGATACA+AGG	-	chr1.2:66899009-66899028	MS.gene20681:CDS	45.0%
	CGGTGACGAAGATTTGTGTT+GGG	-	chr1.2:66898254-66898273	MS.gene20681:CDS	45.0%
	GAAGGTAAAGGTGGATGAGA+AGG	-	chr1.2:66898486-66898505	MS.gene20681:CDS	45.0%
	GCAGTCAACTTCACCTTCTT+TGG	+	chr1.2:66898658-66898677	None:intergenic	45.0%
	GGAATGAGTCGGATCAGTAT+AGG	-	chr1.2:66898296-66898315	MS.gene20681:CDS	45.0%
	GGATGTTACTGAGGAAACTC+CGG	-	chr1.2:66897943-66897962	MS.gene20681:CDS	45.0%
	GGGTGATTGGAACTTCGAAT+GGG	-	chr1.2:66898443-66898462	MS.gene20681:CDS	45.0%
	GTGTTGGAAGGTAAGAATCC+AGG	-	chr1.2:66898727-66898746	MS.gene20681:CDS	45.0%
	TAATCATGGTTGTCCTGTGG+AGG	-	chr1.2:66898114-66898133	MS.gene20681:CDS	45.0%
	TGACGAAGATTTGTGTTGGG+AGG	-	chr1.2:66898257-66898276	MS.gene20681:CDS	45.0%
	TGCAAAGAGGAAGGTAAAGG+TGG	-	chr1.2:66898477-66898496	MS.gene20681:CDS	45.0%
	TGCTGCAAAGAGGAAGGTAA+AGG	-	chr1.2:66898474-66898493	MS.gene20681:CDS	45.0%
	TGGGAATGCGGTCAAGATTT+GGG	-	chr1.2:66898180-66898199	MS.gene20681:CDS	45.0%
	TTCGCCGGATTGTTCTACTA+GGG	-	chr1.2:66898423-66898442	MS.gene20681:CDS	45.0%
	TTCTTCTCAGTATCCTCCAC+CGG	+	chr1.2:66898553-66898572	None:intergenic	45.0%
	TTGAGAGCTGAAGATGGAGA+GGG	+	chr1.2:66897678-66897697	None:intergenic	45.0%
	TTGTTTATTTGCCGGGTGGT+GGG	-	chr1.2:66898140-66898159	MS.gene20681:CDS	45.0%
	TTTGAGAGCTGAAGATGGAG+AGG	+	chr1.2:66897679-66897698	None:intergenic	45.0%
	TTTGTCTGTTGGGTATTCGC+CGG	-	chr1.2:66898408-66898427	MS.gene20681:CDS	45.0%
!	CGAAGGAAAAGGGTGGATTT+GGG	+	chr1.2:66897637-66897656	None:intergenic	45.0%
!	GAAGGAAAAGGGTGGATTTG+GGG	+	chr1.2:66897636-66897655	None:intergenic	45.0%
!	GAGGTTTGGGATTGTGTTCT+TGG	+	chr1.2:66897539-66897558	None:intergenic	45.0%
!	GATGTTGTTTATTTGCCGGG+TGG	-	chr1.2:66898136-66898155	MS.gene20681:CDS	45.0%
!	GTAGTGATGATGCCGTTGTT+AGG	-	chr1.2:66897915-66897934	MS.gene20681:CDS	45.0%
!	GTTTGGGATGTTAGGGTTAG+AGG	-	chr1.2:66898067-66898086	MS.gene20681:CDS	45.0%
!	TGATGGAGGAATTGGTGTCT+AGG	-	chr1.2:66898764-66898783	MS.gene20681:CDS	45.0%
!	TTGATCTAAGACGACGGAGA+CGG	+	chr1.2:66897835-66897854	None:intergenic	45.0%
!!	TTCGGTTTGGAGAATTAGGC+CGG	-	chr1.2:66898531-66898550	MS.gene20681:CDS	45.0%
	AGGTAAGAATCCAGGGCATG+TGG	-	chr1.2:66898735-66898754	MS.gene20681:CDS	50.0%
	ATCGTTACGGAAAGAGACGG+AGG	+	chr1.2:66897747-66897766	None:intergenic	50.0%
	CATGGTGTTTCGTGGCCATA+AGG	-	chr1.2:66897967-66897986	MS.gene20681:CDS	50.0%
	GAAGGATTCGGGGAATGAGT+CGG	-	chr1.2:66898285-66898304	MS.gene20681:CDS	50.0%
	GCAAGGTGAGAAACCATCTG+AGG	-	chr1.2:66898612-66898631	MS.gene20681:CDS	50.0%
	GCACTGTCGAGACAGAGATT+CGG	-	chr1.2:66898980-66898999	MS.gene20681:CDS	50.0%
	GGAGAGGGAAGCTGAATTAG+CGG	+	chr1.2:66897663-66897682	None:intergenic	50.0%
	GGATGATTGCAACAGCTGGT+GGG	-	chr1.2:66898161-66898180	MS.gene20681:CDS	50.0%
	GGTGGAGGATACTGAGAAGA+AGG	-	chr1.2:66898552-66898571	MS.gene20681:CDS	50.0%
	GGTGGATGAGAAGGAAGTTG+GGG	-	chr1.2:66898495-66898514	MS.gene20681:CDS	50.0%
	GGTTAGAGGTGAGACCAACT+CGG	-	chr1.2:66898081-66898100	MS.gene20681:CDS	50.0%
	GTGGGAATGCGGTCAAGATT+TGG	-	chr1.2:66898179-66898198	MS.gene20681:CDS	50.0%
	GTTGTTTATTTGCCGGGTGG+TGG	-	chr1.2:66898139-66898158	MS.gene20681:CDS	50.0%
	TACTGAGGAAACTCCGGTCA+TGG	-	chr1.2:66897949-66897968	MS.gene20681:CDS	50.0%
	TAGATGTGGTGATTCGTCCC+CGG	-	chr1.2:66897997-66898016	MS.gene20681:CDS	50.0%
	TGGATTTGGGGAGAAGGTGA+TGG	+	chr1.2:66897624-66897643	None:intergenic	50.0%
	TGGGAGGCTTGTGTATTCCA+TGG	-	chr1.2:66898216-66898235	MS.gene20681:CDS	50.0%
!	AAAGGGTGGATTTGGGGAGA+AGG	+	chr1.2:66897630-66897649	None:intergenic	50.0%
!	GCGAAGGAAAAGGGTGGATT+TGG	+	chr1.2:66897638-66897657	None:intergenic	50.0%
!	GGAGAAGCTGGAACTGCTTT+TGG	-	chr1.2:66898822-66898841	MS.gene20681:CDS	50.0%
!	GGTTGCTGTGATGGAGGAAT+TGG	-	chr1.2:66898756-66898775	MS.gene20681:CDS	50.0%
!	TGAGTGAGGCTAGTTCGGTT+TGG	-	chr1.2:66898518-66898537	MS.gene20681:CDS	50.0%
!	TGATCTAAGACGACGGAGAC+GGG	+	chr1.2:66897834-66897853	None:intergenic	50.0%
!!	TTGGGGAGAAGGTGATGGAA+GGG	+	chr1.2:66897619-66897638	None:intergenic	50.0%
!!	TTTGGGGAGAAGGTGATGGA+AGG	+	chr1.2:66897620-66897639	None:intergenic	50.0%
	ACGGAAAGAGACGGAGGAGA+CGG	+	chr1.2:66897741-66897760	None:intergenic	55.0%
	ACTCCGGTCATGGTGTTTCG+TGG	-	chr1.2:66897959-66897978	MS.gene20681:CDS	55.0%
	AGGGCATGTGGTTGCTGTGA+TGG	-	chr1.2:66898747-66898766	MS.gene20681:CDS	55.0%
	ATCACAGCAACCACATGCCC+TGG	+	chr1.2:66898748-66898767	None:intergenic	55.0%
	ATTAGCGGCGGCGAAGGAAA+AGG	+	chr1.2:66897648-66897667	None:intergenic	55.0%
	GAAGGAAGTTGGGGTGAGTG+AGG	-	chr1.2:66898504-66898523	MS.gene20681:CDS	55.0%
	GAGGGAAGCTGAATTAGCGG+CGG	+	chr1.2:66897660-66897679	None:intergenic	55.0%
	GCATGTGGTTGCTGTGATGG+AGG	-	chr1.2:66898750-66898769	MS.gene20681:CDS	55.0%
	GCTCTTGTGTGCGTGTTGGA+AGG	-	chr1.2:66898715-66898734	MS.gene20681:CDS	55.0%
	GGGATGATTGCAACAGCTGG+TGG	-	chr1.2:66898160-66898179	MS.gene20681:CDS	55.0%
	GGTGGGATGATTGCAACAGC+TGG	-	chr1.2:66898157-66898176	MS.gene20681:CDS	55.0%
	GGTTTGGAGAATTAGGCCGG+TGG	-	chr1.2:66898534-66898553	MS.gene20681:CDS	55.0%
	GTTGCAATCATCCCACCACC+CGG	+	chr1.2:66898154-66898173	None:intergenic	55.0%
	TAACCCGGAGAGGTCAGAAG+CGG	+	chr1.2:66897783-66897802	None:intergenic	55.0%
	TGAGGCTCTTGTGTGCGTGT+TGG	-	chr1.2:66898711-66898730	MS.gene20681:CDS	55.0%
	TGCAACAGCTGGTGGGAATG+CGG	-	chr1.2:66898168-66898187	MS.gene20681:CDS	55.0%
	TGGCCACGAAACACCATGAC+CGG	+	chr1.2:66897965-66897984	None:intergenic	55.0%
	TGGGAGGATCGGGAAGGATT+CGG	-	chr1.2:66898273-66898292	MS.gene20681:CDS	55.0%
	TTAGCGGCGGCGAAGGAAAA+GGG	+	chr1.2:66897647-66897666	None:intergenic	55.0%
	TTGGAGAATTAGGCCGGTGG+AGG	-	chr1.2:66898537-66898556	MS.gene20681:CDS	55.0%
	TTGTGTTGGGAGGATCGGGA+AGG	-	chr1.2:66898267-66898286	MS.gene20681:CDS	55.0%
!	AGGTGAGACCAACTCGGTGA+TGG	-	chr1.2:66898087-66898106	MS.gene20681:CDS	55.0%
!	CGAGGAGACGAGCATCGTTA+CGG	+	chr1.2:66897760-66897779	None:intergenic	55.0%
!	TGGGGTGAGTGAGGCTAGTT+CGG	-	chr1.2:66898513-66898532	MS.gene20681:CDS	55.0%
!!	GGAGAAGGTGATGGAAGGGA+TGG	+	chr1.2:66897615-66897634	None:intergenic	55.0%
!!	GGGATTTGATTGGTGGTGGG+AGG	-	chr1.2:66898200-66898219	MS.gene20681:CDS	55.0%
!!!	CTGCGCCGCTTTTGTCTGTT+GGG	-	chr1.2:66898398-66898417	MS.gene20681:CDS	55.0%
	AGAGACGGAGGAGACGGTGT+CGG	+	chr1.2:66897735-66897754	None:intergenic	60.0%
	AGCTGAATTAGCGGCGGCGA+AGG	+	chr1.2:66897654-66897673	None:intergenic	60.0%
	CCAACAGACAAAAGCGGCGC+AGG	+	chr1.2:66898400-66898419	None:intergenic	60.0%
	GGAGGATCGGGAAGGATTCG+GGG	-	chr1.2:66898275-66898294	MS.gene20681:CDS	60.0%
	GGGAGGATCGGGAAGGATTC+GGG	-	chr1.2:66898274-66898293	MS.gene20681:CDS	60.0%
	GTCGGAGAAGGAGGAGATGG+TGG	+	chr1.2:66897717-66897736	None:intergenic	60.0%
!	GGAGAAGGAGGAGATGGTGG+CGG	+	chr1.2:66897714-66897733	None:intergenic	60.0%
!	GGTGTCGGAGAAGGAGGAGA+TGG	+	chr1.2:66897720-66897739	None:intergenic	60.0%
!!	AGGAGGAGATGGTGGCGGTT+TGG	+	chr1.2:66897709-66897728	None:intergenic	60.0%
!!!	CCTGCGCCGCTTTTGTCTGT+TGG	-	chr1.2:66898397-66898416	MS.gene20681:CDS	60.0%
	CTCGCCGCTTCTGACCTCTC+CGG	-	chr1.2:66897776-66897795	MS.gene20681:CDS	65.0%
	TCGCCGCTTCTGACCTCTCC+GGG	-	chr1.2:66897777-66897796	MS.gene20681:CDS	65.0%
!	GGAGACGGTGTCGGAGAAGG+AGG	+	chr1.2:66897726-66897745	None:intergenic	65.0%
!	GGAGGAGACGGTGTCGGAGA+AGG	+	chr1.2:66897729-66897748	None:intergenic	65.0%
	CGGAGAGGTCAGAAGCGGCG+AGG	+	chr1.2:66897778-66897797	None:intergenic	70.0%
	GCGGCGGCGAAGGAAAAGGG+TGG	+	chr1.2:66897644-66897663	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	66897479	66899071	66897479	ID=MS.gene20681
chr1.2	mRNA	66897479	66899071	66897479	ID=MS.gene20681.t1;Parent=MS.gene20681
chr1.2	exon	66897479	66899071	66897479	ID=MS.gene20681.t1.exon1;Parent=MS.gene20681.t1
chr1.2	CDS	66897479	66899071	66897479	ID=cds.MS.gene20681.t1;Parent=MS.gene20681.t1

Gene Sequence

>MS.gene20681

ATGGAGGAAGCAAGAGTATCAAAGAACTTCCCAGTTAAACCTAAACTCAAAACCAAACCAAGAACACAATCCCAAACCTCTGAATCCAAATACTGGTCTTCCTTCAAAACCCAACAAATCCCTAAACAATTCTCCATCCCTTCCATCACCTTCTCCCCAAATCCACCCTTTTCCTTCGCCGCCGCTAATTCAGCTTCCCTCTCCATCTTCAGCTCTCAAACTCTTGACCAAACCGCCACCATCTCCTCCTTCTCCGACACCGTCTCCTCCGTCTCTTTCCGTAACGATGCTCGTCTCCTCGCCGCTTCTGACCTCTCCGGGTTAATTCAAATCTTCGATATCAAATCACGTACCCGTCTCCGTCGTCTTAGATCAAACAATCGTCCTGTTAGGTTTGTTCATTATCCGCGTCATGATAAGTTGCATTTAGTTTCTGGTAGTGATGATGCCGTTGTTAGGTATTGGGATGTTACTGAGGAAACTCCGGTCATGGTGTTTCGTGGCCATAAGGATTATGTTAGATGTGGTGATTCGTCCCCGGTTAGTTCTGATTGTTTTGTTACTGGCTCTTATGATCATTTTGTTAAGGTTTGGGATGTTAGGGTTAGAGGTGAGACCAACTCGGTGATGGATTTTAATCATGGTTGTCCTGTGGAGGATGTTGTTTATTTGCCGGGTGGTGGGATGATTGCAACAGCTGGTGGGAATGCGGTCAAGATTTGGGATTTGATTGGTGGTGGGAGGCTTGTGTATTCCATGGAGAGTCATAATAAGACGGTGACGAAGATTTGTGTTGGGAGGATCGGGAAGGATTCGGGGAATGAGTCGGATCAGTATAGGATTATGAGTGTTGGTTTGGATGGTTATTTTAAGGTGTTTGATTACGGTAATATGAAGGTTACTTATTCGATGAGGTATCCTGCGCCGCTTTTGTCTGTTGGGTATTCGCCGGATTGTTCTACTAGGGTGATTGGAACTTCGAATGGGATAATTTATGCTGCAAAGAGGAAGGTAAAGGTGGATGAGAAGGAAGTTGGGGTGAGTGAGGCTAGTTCGGTTTGGAGAATTAGGCCGGTGGAGGATACTGAGAAGAAGGTTTTGAAAACTACTAACTTTAGGTATTTTCAAAGAGGGCAAGGTGAGAAACCATCTGAGGGTGATTACTTGGTTATGAAGCCAAAGAAGGTGAAGTTGACTGCACATGATAAGCTTTTGAATAAGTTTAGGCATGGTGAGGCTCTTGTGTGCGTGTTGGAAGGTAAGAATCCAGGGCATGTGGTTGCTGTGATGGAGGAATTGGTGTCTAGGAAGAAGTTGTTGAGGTGTGTTTCTGATTTAGATTTGGAGAAGCTGGAACTGCTTTTGGCTTTCTTGCATAAGTATTGTACTGTGCCCAAGTATTCTAGTTTGTTAATGGGATTGGCTAATAAGGTTATTGAAATGAGGGCTGATGATATTAGGAATTCTGAAGTTCTCAAGAAGCATATAAGAAATCTCAAGAGCACTGTCGAGACAGAGATTCGGATTCAACAGTCGTTGCAAGAGATACAAGGGATAATTTCTCCTTTGTTGAGAATTGCTGGAAGAAGATGA

Protein sequence

>MS.gene20681.t1

MEEARVSKNFPVKPKLKTKPRTQSQTSESKYWSSFKTQQIPKQFSIPSITFSPNPPFSFAAANSASLSIFSSQTLDQTATISSFSDTVSSVSFRNDARLLAASDLSGLIQIFDIKSRTRLRRLRSNNRPVRFVHYPRHDKLHLVSGSDDAVVRYWDVTEETPVMVFRGHKDYVRCGDSSPVSSDCFVTGSYDHFVKVWDVRVRGETNSVMDFNHGCPVEDVVYLPGGGMIATAGGNAVKIWDLIGGGRLVYSMESHNKTVTKICVGRIGKDSGNESDQYRIMSVGLDGYFKVFDYGNMKVTYSMRYPAPLLSVGYSPDCSTRVIGTSNGIIYAAKRKVKVDEKEVGVSEASSVWRIRPVEDTEKKVLKTTNFRYFQRGQGEKPSEGDYLVMKPKKVKLTAHDKLLNKFRHGEALVCVLEGKNPGHVVAVMEELVSRKKLLRCVSDLDLEKLELLLAFLHKYCTVPKYSSLLMGLANKVIEMRADDIRNSEVLKKHIRNLKSTVETEIRIQQSLQEIQGIISPLLRIAGRR