AlfalfaGEDB | MS.gene39781

M. sativa cultivar XinJiangDaYe / MS.gene39781

KEGG: K11884
GO: GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0097159 GO:1901363
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39781.t1	XP_027336724.1	87.7	219	27	0	1	219	1	219	1.50E-101	379

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39781.t1	A7RP64	57.1	210	85	1	10	219	34	238	6.7e-63	241.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39781.t1	A0A1S3EIE5	87.3	220	27	1	1	219	1	220	1.4e-101	378.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene05648	MS.gene39781	PPI
MS.gene02213	MS.gene39781	PPI
MS.gene85127	MS.gene39781	PPI
MS.gene39781	MS.gene53798	PPI
MS.gene023799	MS.gene39781	PPI
MS.gene57305	MS.gene39781	PPI
MS.gene39781	MS.gene064831	PPI
MS.gene42236	MS.gene39781	PPI
MS.gene27648	MS.gene39781	PPI
MS.gene77076	MS.gene39781	PPI
MS.gene47800	MS.gene39781	PPI
MS.gene01229	MS.gene39781	PPI
MS.gene72163	MS.gene39781	PPI
MS.gene39781	MS.gene83444	PPI
MS.gene39781	MS.gene35140	PPI
MS.gene94952	MS.gene39781	PPI
MS.gene39781	MS.gene030418	PPI
MS.gene030823	MS.gene39781	PPI
MS.gene39781	MS.gene040439	PPI
MS.gene014351	MS.gene39781	PPI
MS.gene39781	MS.gene002256	PPI
MS.gene39781	MS.gene048789	PPI
MS.gene070323	MS.gene39781	PPI
MS.gene76511	MS.gene39781	PPI
MS.gene59369	MS.gene39781	PPI
MS.gene39781	MS.gene028318	PPI
MS.gene39781	MS.gene52775	PPI
MS.gene36329	MS.gene39781	PPI
MS.gene04299	MS.gene39781	PPI
MS.gene023135	MS.gene39781	PPI
MS.gene39781	MS.gene94951	PPI
MS.gene39781	MS.gene067210	PPI
MS.gene39781	MS.gene52173	PPI
MS.gene04195	MS.gene39781	PPI
MS.gene39781	MS.gene72768	PPI
MS.gene028153	MS.gene39781	PPI
MS.gene04300	MS.gene39781	PPI
MS.gene36472	MS.gene39781	PPI
MS.gene04196	MS.gene39781	PPI
MS.gene05650	MS.gene39781	PPI
MS.gene69331	MS.gene39781	PPI
MS.gene82426	MS.gene39781	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39781.t1	MTR_3g061130	86.792	212	28	0	8	219	5	216	8.29e-136	380
MS.gene39781.t1	MTR_5g088410	68.950	219	37	4	1	219	1	188	1.09e-92	270

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39781.t1	AT3G13230	73.709	213	56	0	5	217	3	215	3.07e-119	338
MS.gene39781.t1	AT3G13230	73.709	213	56	0	5	217	3	215	3.07e-119	338

Find 50 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGATCCCAATATATGAATTT+TGG	0.167840	5.4:+69541760	None:intergenic
TTTCCTTTGTGACCTAATTA+AGG	0.193992	5.4:-69541711	MS.gene39781:CDS
TGATGCCAAAATTCATATAT+TGG	0.231356	5.4:-69541765	MS.gene39781:CDS
CTGCAGGTGATCCCTTAATT+AGG	0.312167	5.4:+69541699	None:intergenic
TGCTATTGCTCTTCTGCGTT+TGG	0.322637	5.4:-69541936	MS.gene39781:CDS
CTTGGTCTTACCAAGTTTGC+CGG	0.354396	5.4:+69541829	None:intergenic
AGCATTCTCAATTGCAAACT+TGG	0.360266	5.4:+69541811	None:intergenic
TTTGTCCATGCTTTCATGCT+TGG	0.366098	5.4:-69541974	MS.gene39781:CDS
AGAGGCTCAAACTTCGGCTT+TGG	0.381347	5.4:+69542214	None:intergenic
TTCCTTTGTGACCTAATTAA+GGG	0.390563	5.4:-69541710	MS.gene39781:CDS
CGCATCGCTATACCCCTCTT+AGG	0.397983	5.4:-69542135	MS.gene39781:CDS
AGAAGTAACTGCTCTCAATC+TGG	0.402249	5.4:+69541664	None:intergenic
GATGCCAAAATTCATATATT+GGG	0.412346	5.4:-69541764	MS.gene39781:CDS
TCATGAGATTTCTACTGGTC+AGG	0.417970	5.4:-69542185	MS.gene39781:CDS
TCCGAGGCTTCAAATTCATT+CGG	0.426646	5.4:+69542059	None:intergenic
TGTTGAGTCCTTTGAGATCA+AGG	0.427401	5.4:-69541903	MS.gene39781:CDS
GGCTCAAACTTCGGCTTTGG+TGG	0.437317	5.4:+69542217	None:intergenic
GGAAGATTGTCCGGCAAACT+TGG	0.443007	5.4:-69541839	MS.gene39781:CDS
CGTGCAATTGGAAGATTGTC+CGG	0.459600	5.4:-69541848	MS.gene39781:CDS
GATCACTTGTCACGTGCAAT+TGG	0.463391	5.4:-69541860	MS.gene39781:CDS
TTGTAAACATTAAGATTGCT+AGG	0.464495	5.4:-69541736	MS.gene39781:CDS
ATCATCATCTGCTCATATAT+AGG	0.466120	5.4:+69542088	None:intergenic
TCCGAATGAATTTGAAGCCT+CGG	0.466292	5.4:-69542060	MS.gene39781:CDS
TGTAAACATTAAGATTGCTA+GGG	0.485384	5.4:-69541735	MS.gene39781:CDS
CCCTCTTAGGAAAGCTTGGA+TGG	0.488099	5.4:-69542122	MS.gene39781:CDS
GGTTTCAGAGGCTCAAACTT+CGG	0.488900	5.4:+69542208	None:intergenic
TCCATCCAAGCTTTCCTAAG+AGG	0.494180	5.4:+69542121	None:intergenic
GAGAGCAGTTACTTCTAAGC+TGG	0.504373	5.4:-69541657	MS.gene39781:CDS
CTAATTAAGGGATCACCTGC+AGG	0.506285	5.4:-69541698	MS.gene39781:CDS
ATACCCCTCTTAGGAAAGCT+TGG	0.506546	5.4:-69542126	MS.gene39781:CDS
CCATCCAAGCTTTCCTAAGA+GGG	0.509832	5.4:+69542122	None:intergenic
AAACCTCATGAGATTTCTAC+TGG	0.521426	5.4:-69542190	MS.gene39781:CDS
AAGGATGTTAAGACACTTCG+TGG	0.522124	5.4:-69541884	MS.gene39781:CDS
TAAGGGATCACCTGCAGGAA+AGG	0.533097	5.4:-69541693	MS.gene39781:CDS
CTGGAACAGACCTTTCCTGC+AGG	0.538321	5.4:+69541683	None:intergenic
GTCTAACAAAACATATTCGA+TGG	0.546689	5.4:-69542275	MS.gene39781:CDS
GGAAAATTGAGCTGAAGACA+AGG	0.581201	5.4:-69542039	MS.gene39781:CDS
CAAAACCAAGCATGAAAGCA+TGG	0.604734	5.4:+69541969	None:intergenic
CTTAACATCCTTGATCTCAA+AGG	0.606154	5.4:+69541895	None:intergenic
GAAATCTCATGAGGTTTCAG+AGG	0.613445	5.4:+69542196	None:intergenic
GTAAACATTAAGATTGCTAG+GGG	0.620738	5.4:-69541734	MS.gene39781:CDS
ATCCCTTAATTAGGTCACAA+AGG	0.627414	5.4:+69541708	None:intergenic
TTGAGAATGCTTCTAAGACG+AGG	0.657784	5.4:-69541799	MS.gene39781:CDS
AGAGGGGTATAGCGATGCGG+TGG	0.669136	5.4:+69542139	None:intergenic
TGGTCAGGTTCAGTTCCGCA+AGG	0.669299	5.4:-69542170	MS.gene39781:CDS
CATCCAAGCTTTCCTAAGAG+GGG	0.671655	5.4:+69542123	None:intergenic
TGACCAGTAGAAATCTCATG+AGG	0.705113	5.4:+69542187	None:intergenic
CTAAGAGGGGTATAGCGATG+CGG	0.719010	5.4:+69542136	None:intergenic
TTACTTCTAAGCTGGCAGAG+AGG	0.721447	5.4:-69541649	MS.gene39781:CDS
GCGGTGGAACTGATACCTTG+CGG	0.766529	5.4:+69542155	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GATGCCAAAATTCATATATT+GGG	-	chr5.4:69542159-69542178	MS.gene39781:CDS	25.0%
!	TGATGCCAAAATTCATATAT+TGG	-	chr5.4:69542158-69542177	MS.gene39781:CDS	25.0%
!	TGTAAACATTAAGATTGCTA+GGG	-	chr5.4:69542188-69542207	MS.gene39781:CDS	25.0%
!	TTGTAAACATTAAGATTGCT+AGG	-	chr5.4:69542187-69542206	MS.gene39781:CDS	25.0%
!!	AGATCCCAATATATGAATTT+TGG	+	chr5.4:69542166-69542185	None:intergenic	25.0%
	ATCATCATCTGCTCATATAT+AGG	+	chr5.4:69541838-69541857	None:intergenic	30.0%
	GTAAACATTAAGATTGCTAG+GGG	-	chr5.4:69542189-69542208	MS.gene39781:CDS	30.0%
	GTCTAACAAAACATATTCGA+TGG	-	chr5.4:69541648-69541667	MS.gene39781:CDS	30.0%
	TTCCTTTGTGACCTAATTAA+GGG	-	chr5.4:69542213-69542232	MS.gene39781:CDS	30.0%
	TTTCCTTTGTGACCTAATTA+AGG	-	chr5.4:69542212-69542231	MS.gene39781:CDS	30.0%
	AAACCTCATGAGATTTCTAC+TGG	-	chr5.4:69541733-69541752	MS.gene39781:CDS	35.0%
	AGCATTCTCAATTGCAAACT+TGG	+	chr5.4:69542115-69542134	None:intergenic	35.0%
	ATCCCTTAATTAGGTCACAA+AGG	+	chr5.4:69542218-69542237	None:intergenic	35.0%
	CTTAACATCCTTGATCTCAA+AGG	+	chr5.4:69542031-69542050	None:intergenic	35.0%
!	TTTTGCAAGTTGCTGATATC+AGG	+	chr5.4:69541919-69541938	None:intergenic	35.0%
	AAGGATGTTAAGACACTTCG+TGG	-	chr5.4:69542039-69542058	MS.gene39781:CDS	40.0%
	AGAAGTAACTGCTCTCAATC+TGG	+	chr5.4:69542262-69542281	None:intergenic	40.0%
	CAAAACCAAGCATGAAAGCA+TGG	+	chr5.4:69541957-69541976	None:intergenic	40.0%
	GAAATCTCATGAGGTTTCAG+AGG	+	chr5.4:69541730-69541749	None:intergenic	40.0%
	GGAAAATTGAGCTGAAGACA+AGG	-	chr5.4:69541884-69541903	MS.gene39781:CDS	40.0%
	TCATGAGATTTCTACTGGTC+AGG	-	chr5.4:69541738-69541757	MS.gene39781:CDS	40.0%
	TCCGAGGCTTCAAATTCATT+CGG	+	chr5.4:69541867-69541886	None:intergenic	40.0%
	TGACCAGTAGAAATCTCATG+AGG	+	chr5.4:69541739-69541758	None:intergenic	40.0%
	TTTGTCCATGCTTTCATGCT+TGG	-	chr5.4:69541949-69541968	MS.gene39781:CDS	40.0%
!	TCCGAATGAATTTGAAGCCT+CGG	-	chr5.4:69541863-69541882	MS.gene39781:CDS	40.0%
!!	TGTTGAGTCCTTTGAGATCA+AGG	-	chr5.4:69542020-69542039	MS.gene39781:CDS	40.0%
!!	TTGAGAATGCTTCTAAGACG+AGG	-	chr5.4:69542124-69542143	MS.gene39781:CDS	40.0%
	ATACCCCTCTTAGGAAAGCT+TGG	-	chr5.4:69541797-69541816	MS.gene39781:CDS	45.0%
	CATCCAAGCTTTCCTAAGAG+GGG	+	chr5.4:69541803-69541822	None:intergenic	45.0%
	CCATCCAAGCTTTCCTAAGA+GGG	+	chr5.4:69541804-69541823	None:intergenic	45.0%
	CGTGCAATTGGAAGATTGTC+CGG	-	chr5.4:69542075-69542094	MS.gene39781:CDS	45.0%
	CTAATTAAGGGATCACCTGC+AGG	-	chr5.4:69542225-69542244	MS.gene39781:CDS	45.0%
	CTGCAGGTGATCCCTTAATT+AGG	+	chr5.4:69542227-69542246	None:intergenic	45.0%
	GAGAGCAGTTACTTCTAAGC+TGG	-	chr5.4:69542266-69542285	MS.gene39781:CDS	45.0%
	GATCACTTGTCACGTGCAAT+TGG	-	chr5.4:69542063-69542082	MS.gene39781:CDS	45.0%
	GGTTTCAGAGGCTCAAACTT+CGG	+	chr5.4:69541718-69541737	None:intergenic	45.0%
	TCCATCCAAGCTTTCCTAAG+AGG	+	chr5.4:69541805-69541824	None:intergenic	45.0%
	TGCTATTGCTCTTCTGCGTT+TGG	-	chr5.4:69541987-69542006	MS.gene39781:CDS	45.0%
	TTACTTCTAAGCTGGCAGAG+AGG	-	chr5.4:69542274-69542293	MS.gene39781:CDS	45.0%
!	GTCTTCAGCTCAATTTTCCG+AGG	+	chr5.4:69541883-69541902	None:intergenic	45.0%
!!	CTTGGTCTTACCAAGTTTGC+CGG	+	chr5.4:69542097-69542116	None:intergenic	45.0%
	AGAGGCTCAAACTTCGGCTT+TGG	+	chr5.4:69541712-69541731	None:intergenic	50.0%
	CCCTCTTAGGAAAGCTTGGA+TGG	-	chr5.4:69541801-69541820	MS.gene39781:CDS	50.0%
	GGAAGATTGTCCGGCAAACT+TGG	-	chr5.4:69542084-69542103	MS.gene39781:CDS	50.0%
	TAAGGGATCACCTGCAGGAA+AGG	-	chr5.4:69542230-69542249	MS.gene39781:CDS	50.0%
!	CTAAGAGGGGTATAGCGATG+CGG	+	chr5.4:69541790-69541809	None:intergenic	50.0%
	CGCATCGCTATACCCCTCTT+AGG	-	chr5.4:69541788-69541807	MS.gene39781:CDS	55.0%
	CTGGAACAGACCTTTCCTGC+AGG	+	chr5.4:69542243-69542262	None:intergenic	55.0%
	GCGGTGGAACTGATACCTTG+CGG	+	chr5.4:69541771-69541790	None:intergenic	55.0%
	TGGTCAGGTTCAGTTCCGCA+AGG	-	chr5.4:69541753-69541772	MS.gene39781:CDS	55.0%
!	GGCTCAAACTTCGGCTTTGG+TGG	+	chr5.4:69541709-69541728	None:intergenic	55.0%
!	AGAGGGGTATAGCGATGCGG+TGG	+	chr5.4:69541787-69541806	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	69541643	69542302	69541643	ID=MS.gene39781
chr5.4	mRNA	69541643	69542302	69541643	ID=MS.gene39781.t1;Parent=MS.gene39781
chr5.4	exon	69541643	69542302	69541643	ID=MS.gene39781.t1.exon1;Parent=MS.gene39781.t1
chr5.4	CDS	69541643	69542302	69541643	ID=cds.MS.gene39781.t1;Parent=MS.gene39781.t1

Gene Sequence

>MS.gene39781

ATGCAGTCTAACAAAACATATTCGATGGAAGTTGACACTGTCTCATCTGAATCAAAAGTTTTGCCACCAAAGCCGAAGTTTGAGCCTCTGAAACCTCATGAGATTTCTACTGGTCAGGTTCAGTTCCGCAAGGTATCAGTTCCACCGCATCGCTATACCCCTCTTAGGAAAGCTTGGATGGACATCTATACTCCTATATATGAGCAGATGATGATTGACATCCGAATGAATTTGAAGCCTCGGAAAATTGAGCTGAAGACAAGGCACGATACACCTGATATCAGCAACTTGCAAAAATGTGCTGATTTTGTCCATGCTTTCATGCTTGGTTTTGATGTTATAGATGCTATTGCTCTTCTGCGTTTGGATGAGCTCTATGTTGAGTCCTTTGAGATCAAGGATGTTAAGACACTTCGTGGCGATCACTTGTCACGTGCAATTGGAAGATTGTCCGGCAAACTTGGTAAGACCAAGTTTGCAATTGAGAATGCTTCTAAGACGAGGATTGTGATTGCTGATGCCAAAATTCATATATTGGGATCTTTTGTAAACATTAAGATTGCTAGGGGTTTCCTTTGTGACCTAATTAAGGGATCACCTGCAGGAAAGGTCTGTTCCAGATTGAGAGCAGTTACTTCTAAGCTGGCAGAGAGGTTTTGA

Protein sequence

>MS.gene39781.t1

MQSNKTYSMEVDTVSSESKVLPPKPKFEPLKPHEISTGQVQFRKVSVPPHRYTPLRKAWMDIYTPIYEQMMIDIRMNLKPRKIELKTRHDTPDISNLQKCADFVHAFMLGFDVIDAIALLRLDELYVESFEIKDVKTLRGDHLSRAIGRLSGKLGKTKFAIENASKTRIVIADAKIHILGSFVNIKIARGFLCDLIKGSPAGKVCSRLRAVTSKLAERF