AlfalfaGEDB | MS.gene016898

M. sativa cultivar XinJiangDaYe / MS.gene016898

KEGG: K11884
GO: GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0097159 GO:1901363
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene016898.t1	XP_027336724.1	86.3	219	30	0	1	219	1	219	6.20E-100	373.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene016898.t1	A7RP64	57.1	210	85	1	10	219	34	238	1.5e-62	240.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene016898.t1	A0A1S3EIE5	85.9	220	30	1	1	219	1	220	5.8e-100	373.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene016898	MS.gene77076	PPI
MS.gene016898	MS.gene064831	PPI
MS.gene016898	MS.gene030418	PPI
MS.gene016898	MS.gene05648	PPI
MS.gene36329	MS.gene016898	PPI
MS.gene023135	MS.gene016898	PPI
MS.gene02213	MS.gene016898	PPI
MS.gene028153	MS.gene016898	PPI
MS.gene016898	MS.gene47800	PPI
MS.gene42236	MS.gene016898	PPI
MS.gene016898	MS.gene028318	PPI
MS.gene016898	MS.gene83444	PPI
MS.gene016898	MS.gene35140	PPI
MS.gene016898	MS.gene52775	PPI
MS.gene05650	MS.gene016898	PPI
MS.gene016898	MS.gene94951	PPI
MS.gene016898	MS.gene52173	PPI
MS.gene016898	MS.gene040439	PPI
MS.gene016898	MS.gene002256	PPI
MS.gene016898	MS.gene048789	PPI
MS.gene016898	MS.gene04300	PPI
MS.gene27648	MS.gene016898	PPI
MS.gene016898	MS.gene023799	PPI
MS.gene016898	MS.gene067210	PPI
MS.gene016898	MS.gene72768	PPI
MS.gene016898	MS.gene070323	PPI
MS.gene016898	MS.gene53798	PPI
MS.gene016898	MS.gene36472	PPI
MS.gene72163	MS.gene016898	PPI
MS.gene59369	MS.gene016898	PPI
MS.gene016898	MS.gene030823	PPI
MS.gene016898	MS.gene69331	PPI
MS.gene016898	MS.gene85127	PPI
MS.gene04195	MS.gene016898	PPI
MS.gene016898	MS.gene57305	PPI
MS.gene014351	MS.gene016898	PPI
MS.gene016898	MS.gene82426	PPI
MS.gene016898	MS.gene04299	PPI
MS.gene016898	MS.gene94952	PPI
MS.gene04196	MS.gene016898	PPI
MS.gene76511	MS.gene016898	PPI
MS.gene016898	MS.gene01229	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene016898.t1	MTR_3g061130	84.579	214	33	0	6	219	3	216	1.67e-133	374
MS.gene016898.t1	MTR_5g088410	68.950	219	37	4	1	219	1	188	1.52e-92	269

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene016898.t1	AT3G13230	71.831	213	60	0	5	217	3	215	9.67e-117	332
MS.gene016898.t1	AT3G13230	71.831	213	60	0	5	217	3	215	9.67e-117	332

Find 46 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGATCCCAATATATGAATTT+TGG	0.167840	5.2:+74965061	None:intergenic
GATTGACATCCGAATCAATT+TGG	0.230375	5.2:-74965369	MS.gene016898:CDS
TGATGCCAAAATTCATATAT+TGG	0.231356	5.2:-74965066	MS.gene016898:CDS
CTGCAGGTGATCCCTTAATT+AGG	0.312167	5.2:+74965000	None:intergenic
TGCTATTGCTCTTCTGCGTT+TGG	0.322637	5.2:-74965237	MS.gene016898:CDS
CTTGGTCTTACCAAGTTTGC+CGG	0.354396	5.2:+74965130	None:intergenic
AGCATTCTCAATTGCAAACT+TGG	0.360266	5.2:+74965112	None:intergenic
TTTGTCCATGCTTTCATGCT+TGG	0.366098	5.2:-74965275	MS.gene016898:CDS
AGAGGCTCAAACTTCGGCTT+TGG	0.371216	5.2:+74965515	None:intergenic
TTTCTTTGTGACCTAATTAA+GGG	0.383724	5.2:-74965011	MS.gene016898:CDS
CGCATCGTTATACCCCTCTT+AGG	0.387027	5.2:-74965436	MS.gene016898:CDS
AGAAGTAACTGCTCTCAATC+TGG	0.402249	5.2:+74964965	None:intergenic
GATGCCAAAATTCATATATT+GGG	0.412346	5.2:-74965065	MS.gene016898:CDS
TCATGAGATTTCTACTGGTC+AGG	0.417970	5.2:-74965486	MS.gene016898:CDS
TTCGAGGCTCCAAATTGATT+CGG	0.423831	5.2:+74965360	None:intergenic
TGTTGAGTCCTTTGAGATCA+AGG	0.427401	5.2:-74965204	MS.gene016898:CDS
GGAAGATTGTCCGGCAAACT+TGG	0.443007	5.2:-74965140	MS.gene016898:CDS
GATCACTTGTCTCGTGCAAT+TGG	0.453450	5.2:-74965161	MS.gene016898:CDS
TTGTAAACATTAAGATTGCT+AGG	0.464495	5.2:-74965037	MS.gene016898:CDS
ATCATCATCTGCTCATATAT+AGG	0.466120	5.2:+74965389	None:intergenic
CGTGCAATTGGAAGATTGTC+CGG	0.470718	5.2:-74965149	MS.gene016898:CDS
GGCTCAAACTTCGGCTTTGG+CGG	0.485174	5.2:+74965518	None:intergenic
TGTAAACATTAAGATTGCTA+GGG	0.485384	5.2:-74965036	MS.gene016898:CDS
CCCTCTTAGGAAAGCTTGGA+TGG	0.488099	5.2:-74965423	MS.gene016898:CDS
GGTTTCAGAGGCTCAAACTT+CGG	0.488900	5.2:+74965509	None:intergenic
TCCATCCAAGCTTTCCTAAG+AGG	0.494180	5.2:+74965422	None:intergenic
GAGAGCAGTTACTTCTAAGC+TGG	0.504373	5.2:-74964958	MS.gene016898:CDS
CTAATTAAGGGATCACCTGC+AGG	0.506285	5.2:-74964999	MS.gene016898:CDS
ATACCCCTCTTAGGAAAGCT+TGG	0.509210	5.2:-74965427	MS.gene016898:CDS
CCATCCAAGCTTTCCTAAGA+GGG	0.509832	5.2:+74965423	None:intergenic
AAACCTCATGAGATTTCTAC+TGG	0.521426	5.2:-74965491	MS.gene016898:CDS
AAGGATGTTAAGACACTTCG+TGG	0.522124	5.2:-74965185	MS.gene016898:CDS
TAAGGGATCACCTGCAGGAA+AGG	0.533097	5.2:-74964994	MS.gene016898:CDS
CTGGAACAGACCTTTCCTGC+AGG	0.538321	5.2:+74964984	None:intergenic
CAAAACCAAGCATGAAAGCA+TGG	0.604734	5.2:+74965270	None:intergenic
CTTAACATCCTTGATCTCAA+AGG	0.606154	5.2:+74965196	None:intergenic
GAAATCTCATGAGGTTTCAG+AGG	0.613445	5.2:+74965497	None:intergenic
GTAAACATTAAGATTGCTAG+GGG	0.620738	5.2:-74965035	MS.gene016898:CDS
TTGAGAATGCTTCTAAGACG+AGG	0.657784	5.2:-74965100	MS.gene016898:CDS
AGAGGGGTATAACGATGCGG+TGG	0.666984	5.2:+74965440	None:intergenic
TGGTCAGGTTCAGTTCCGCA+AGG	0.669299	5.2:-74965471	MS.gene016898:CDS
CATCCAAGCTTTCCTAAGAG+GGG	0.671655	5.2:+74965424	None:intergenic
TGACCAGTAGAAATCTCATG+AGG	0.705113	5.2:+74965488	None:intergenic
CTAAGAGGGGTATAACGATG+CGG	0.721029	5.2:+74965437	None:intergenic
TTACTTCTAAGCTGGCAGAG+AGG	0.721447	5.2:-74964950	MS.gene016898:CDS
GCGGTGGAACTGATACCTTG+CGG	0.766529	5.2:+74965456	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GATGCCAAAATTCATATATT+GGG	-	chr5.2:74965460-74965479	MS.gene016898:CDS	25.0%
!	TGATGCCAAAATTCATATAT+TGG	-	chr5.2:74965459-74965478	MS.gene016898:CDS	25.0%
!	TGTAAACATTAAGATTGCTA+GGG	-	chr5.2:74965489-74965508	MS.gene016898:CDS	25.0%
!	TTGTAAACATTAAGATTGCT+AGG	-	chr5.2:74965488-74965507	MS.gene016898:CDS	25.0%
!	TTTCTTTGTGACCTAATTAA+GGG	-	chr5.2:74965514-74965533	MS.gene016898:CDS	25.0%
!!	AGATCCCAATATATGAATTT+TGG	+	chr5.2:74965467-74965486	None:intergenic	25.0%
!!	TTTTCTTTGTGACCTAATTA+AGG	-	chr5.2:74965513-74965532	MS.gene016898:CDS	25.0%
	ATCATCATCTGCTCATATAT+AGG	+	chr5.2:74965139-74965158	None:intergenic	30.0%
	GTAAACATTAAGATTGCTAG+GGG	-	chr5.2:74965490-74965509	MS.gene016898:CDS	30.0%
!!	GAAAAATTGAGTTGAAGACA+AGG	-	chr5.2:74965185-74965204	MS.gene016898:CDS	30.0%
	AAACCTCATGAGATTTCTAC+TGG	-	chr5.2:74965034-74965053	MS.gene016898:CDS	35.0%
	AGCATTCTCAATTGCAAACT+TGG	+	chr5.2:74965416-74965435	None:intergenic	35.0%
	CTTAACATCCTTGATCTCAA+AGG	+	chr5.2:74965332-74965351	None:intergenic	35.0%
	GATTGACATCCGAATCAATT+TGG	-	chr5.2:74965156-74965175	MS.gene016898:CDS	35.0%
!	GTCTTCAACTCAATTTTTCG+AGG	+	chr5.2:74965184-74965203	None:intergenic	35.0%
!	TTTTGCAAGTTGCTGATATC+AGG	+	chr5.2:74965220-74965239	None:intergenic	35.0%
	AAGGATGTTAAGACACTTCG+TGG	-	chr5.2:74965340-74965359	MS.gene016898:CDS	40.0%
	AGAAGTAACTGCTCTCAATC+TGG	+	chr5.2:74965563-74965582	None:intergenic	40.0%
	CAAAACCAAGCATGAAAGCA+TGG	+	chr5.2:74965258-74965277	None:intergenic	40.0%
	GAAATCTCATGAGGTTTCAG+AGG	+	chr5.2:74965031-74965050	None:intergenic	40.0%
	TCATGAGATTTCTACTGGTC+AGG	-	chr5.2:74965039-74965058	MS.gene016898:CDS	40.0%
	TGACCAGTAGAAATCTCATG+AGG	+	chr5.2:74965040-74965059	None:intergenic	40.0%
	TTCGAGGCTCCAAATTGATT+CGG	+	chr5.2:74965168-74965187	None:intergenic	40.0%
	TTTGTCCATGCTTTCATGCT+TGG	-	chr5.2:74965250-74965269	MS.gene016898:CDS	40.0%
!!	TGTTGAGTCCTTTGAGATCA+AGG	-	chr5.2:74965321-74965340	MS.gene016898:CDS	40.0%
!!	TTGAGAATGCTTCTAAGACG+AGG	-	chr5.2:74965425-74965444	MS.gene016898:CDS	40.0%
	ATACCCCTCTTAGGAAAGCT+TGG	-	chr5.2:74965098-74965117	MS.gene016898:CDS	45.0%
	CATCCAAGCTTTCCTAAGAG+GGG	+	chr5.2:74965104-74965123	None:intergenic	45.0%
	CCATCCAAGCTTTCCTAAGA+GGG	+	chr5.2:74965105-74965124	None:intergenic	45.0%
	CGTGCAATTGGAAGATTGTC+CGG	-	chr5.2:74965376-74965395	MS.gene016898:CDS	45.0%
	CTAATTAAGGGATCACCTGC+AGG	-	chr5.2:74965526-74965545	MS.gene016898:CDS	45.0%
	CTGCAGGTGATCCCTTAATT+AGG	+	chr5.2:74965528-74965547	None:intergenic	45.0%
	GAGAGCAGTTACTTCTAAGC+TGG	-	chr5.2:74965567-74965586	MS.gene016898:CDS	45.0%
	GATCACTTGTCTCGTGCAAT+TGG	-	chr5.2:74965364-74965383	MS.gene016898:CDS	45.0%
	GGTTTCAGAGGCTCAAACTT+CGG	+	chr5.2:74965019-74965038	None:intergenic	45.0%
	TCCATCCAAGCTTTCCTAAG+AGG	+	chr5.2:74965106-74965125	None:intergenic	45.0%
	TGCTATTGCTCTTCTGCGTT+TGG	-	chr5.2:74965288-74965307	MS.gene016898:CDS	45.0%
	TTACTTCTAAGCTGGCAGAG+AGG	-	chr5.2:74965575-74965594	MS.gene016898:CDS	45.0%
!	CTAAGAGGGGTATAACGATG+CGG	+	chr5.2:74965091-74965110	None:intergenic	45.0%
!!	CTTGGTCTTACCAAGTTTGC+CGG	+	chr5.2:74965398-74965417	None:intergenic	45.0%
	AGAGGCTCAAACTTCGGCTT+TGG	+	chr5.2:74965013-74965032	None:intergenic	50.0%
	CCCTCTTAGGAAAGCTTGGA+TGG	-	chr5.2:74965102-74965121	MS.gene016898:CDS	50.0%
	CGCATCGTTATACCCCTCTT+AGG	-	chr5.2:74965089-74965108	MS.gene016898:CDS	50.0%
	GGAAGATTGTCCGGCAAACT+TGG	-	chr5.2:74965385-74965404	MS.gene016898:CDS	50.0%
	TAAGGGATCACCTGCAGGAA+AGG	-	chr5.2:74965531-74965550	MS.gene016898:CDS	50.0%
	CTGGAACAGACCTTTCCTGC+AGG	+	chr5.2:74965544-74965563	None:intergenic	55.0%
	GCGGTGGAACTGATACCTTG+CGG	+	chr5.2:74965072-74965091	None:intergenic	55.0%
	TGGTCAGGTTCAGTTCCGCA+AGG	-	chr5.2:74965054-74965073	MS.gene016898:CDS	55.0%
!	AGAGGGGTATAACGATGCGG+TGG	+	chr5.2:74965088-74965107	None:intergenic	55.0%
!	GGCTCAAACTTCGGCTTTGG+CGG	+	chr5.2:74965010-74965029	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	74964944	74965603	74964944	ID=MS.gene016898
chr5.2	mRNA	74964944	74965603	74964944	ID=MS.gene016898.t1;Parent=MS.gene016898
chr5.2	exon	74964944	74965603	74964944	ID=MS.gene016898.t1.exon1;Parent=MS.gene016898.t1
chr5.2	CDS	74964944	74965603	74964944	ID=cds.MS.gene016898.t1;Parent=MS.gene016898.t1

Gene Sequence

>MS.gene016898

ATGCAGTCTAACAAAACATCTTCGATAGAAGTTGACACTGTCTCATCTGAATCAAAAGTTTTGCCGCCAAAGCCGAAGTTTGAGCCTCTGAAACCTCATGAGATTTCTACTGGTCAGGTTCAGTTCCGCAAGGTATCAGTTCCACCGCATCGTTATACCCCTCTTAGGAAAGCTTGGATGGACATCTGTACTCCTATATATGAGCAGATGATGATTGACATCCGAATCAATTTGGAGCCTCGAAAAATTGAGTTGAAGACAAGGCATGATACACCTGATATCAGCAACTTGCAAAAATGTGCTGATTTTGTCCATGCTTTCATGCTTGGTTTTGATGTTATAGATGCTATTGCTCTTCTGCGTTTGGATGAGCTCTATGTTGAGTCCTTTGAGATCAAGGATGTTAAGACACTTCGTGGCGATCACTTGTCTCGTGCAATTGGAAGATTGTCCGGCAAACTTGGTAAGACCAAGTTTGCAATTGAGAATGCTTCTAAGACGAGGATTGTGATTGCTGATGCCAAAATTCATATATTGGGATCTTTTGTAAACATTAAGATTGCTAGGGGTTTTCTTTGTGACCTAATTAAGGGATCACCTGCAGGAAAGGTCTGTTCCAGATTGAGAGCAGTTACTTCTAAGCTGGCAGAGAGGTTTTGA

Protein sequence

>MS.gene016898.t1

MQSNKTSSIEVDTVSSESKVLPPKPKFEPLKPHEISTGQVQFRKVSVPPHRYTPLRKAWMDICTPIYEQMMIDIRINLEPRKIELKTRHDTPDISNLQKCADFVHAFMLGFDVIDAIALLRLDELYVESFEIKDVKTLRGDHLSRAIGRLSGKLGKTKFAIENASKTRIVIADAKIHILGSFVNIKIARGFLCDLIKGSPAGKVCSRLRAVTSKLAERF