AlfalfaGEDB | MS.gene08073

M. sativa cultivar XinJiangDaYe / MS.gene08073

KEGG: K11884
GO: GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0097159 GO:1901363
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08073.t1	XP_003600436.2	99.1	216	2	0	1	216	1	216	1.10E-114	422.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08073.t1	A7RP64	60.5	210	78	1	7	216	34	238	7.6e-67	255.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08073.t1	I3S062	99.1	216	2	0	1	216	1	216	8.2e-115	422.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene05721	MS.gene08073	0.855984	4.37E-62	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene08073	MS.gene59369	PPI
MS.gene08073	MS.gene82426	PPI
MS.gene08073	MS.gene04299	PPI
MS.gene08073	MS.gene94952	PPI
MS.gene08073	MS.gene028153	PPI
MS.gene08073	MS.gene35140	PPI
MS.gene08073	MS.gene77076	PPI
MS.gene08073	MS.gene030418	PPI
MS.gene08073	MS.gene05648	PPI
MS.gene08073	MS.gene02213	PPI
MS.gene08073	MS.gene04195	PPI
MS.gene08073	MS.gene040439	PPI
MS.gene08073	MS.gene002256	PPI
MS.gene08073	MS.gene47800	PPI
MS.gene08073	MS.gene048789	PPI
MS.gene08073	MS.gene27648	PPI
MS.gene08073	MS.gene04300	PPI
MS.gene08073	MS.gene028318	PPI
MS.gene08073	MS.gene83444	PPI
MS.gene08073	MS.gene52775	PPI
MS.gene08073	MS.gene94951	PPI
MS.gene08073	MS.gene014351	PPI
MS.gene08073	MS.gene36329	PPI
MS.gene08073	MS.gene023799	PPI
MS.gene08073	MS.gene52173	PPI
MS.gene08073	MS.gene72768	PPI
MS.gene08073	MS.gene76511	PPI
MS.gene08073	MS.gene023135	PPI
MS.gene08073	MS.gene42236	PPI
MS.gene08073	MS.gene69331	PPI
MS.gene08073	MS.gene04196	PPI
MS.gene08073	MS.gene72163	PPI
MS.gene08073	MS.gene067210	PPI
MS.gene08073	MS.gene070323	PPI
MS.gene08073	MS.gene53798	PPI
MS.gene08073	MS.gene36472	PPI
MS.gene08073	MS.gene01229	PPI
MS.gene08073	MS.gene064831	PPI
MS.gene08073	MS.gene030823	PPI
MS.gene08073	MS.gene85127	PPI
MS.gene08073	MS.gene05650	PPI
MS.gene08073	MS.gene57305	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08073.t1	MTR_3g061130	99.074	216	2	0	1	216	1	216	3.04e-160	441
MS.gene08073.t1	MTR_5g088410	60.465	215	52	5	3	216	6	188	2.43e-75	225

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08073.t1	AT3G13230	75.814	215	51	1	1	214	1	215	4.38e-123	347
MS.gene08073.t1	AT3G13230	75.814	215	51	1	1	214	1	215	4.38e-123	347

Find 47 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCTCTTTGTTACCTAATTA+TGG	0.104484	3.3:-49285736	MS.gene08073:CDS
AGATCCCAGAATGTGAATTT+TGG	0.238915	3.3:+49285785	None:intergenic
TGATACCAAAATTCACATTC+TGG	0.283493	3.3:-49285790	MS.gene08073:CDS
CTGCAGGTGATCCCATAATT+AGG	0.290266	3.3:+49285724	None:intergenic
AGTTGAGAGCCGTTACTTCT+AGG	0.295245	3.3:-49285686	MS.gene08073:CDS
TGCTATTGCTATTCTGCGTT+TGG	0.312006	3.3:-49285961	MS.gene08073:CDS
TCTCTTTGTTACCTAATTAT+GGG	0.325990	3.3:-49285735	MS.gene08073:CDS
TTTGTTCATGCTTTCATGTT+GGG	0.347801	3.3:-49285999	MS.gene08073:CDS
GGCTCAAACTTTGGCTTTGG+AGG	0.352039	3.3:+49286242	None:intergenic
AGAGGCTCAAACTTTGGCTT+TGG	0.356673	3.3:+49286239	None:intergenic
ATACACCTCTCAAGAAAGTT+TGG	0.369468	3.3:-49286151	MS.gene08073:CDS
GATCACTTGTCCCGTGCTAT+TGG	0.373903	3.3:-49285885	MS.gene08073:CDS
CGTGCTATTGGAAGATTATC+TGG	0.376662	3.3:-49285873	MS.gene08073:CDS
TGCATTCTCAATTGTAAACT+TGG	0.386651	3.3:+49285836	None:intergenic
GGTTTCAGAGGCTCAAACTT+TGG	0.395402	3.3:+49286233	None:intergenic
TTCGACCCTTCAAATTCATT+CGG	0.417898	3.3:+49286084	None:intergenic
TGTTGAGTCCTTTGAGATCA+AGG	0.427401	3.3:-49285928	MS.gene08073:CDS
ATTGGAAGATTATCTGGAAA+AGG	0.469137	3.3:-49285867	MS.gene08073:CDS
TTGCAAACATCAAGATTGCT+AGG	0.471818	3.3:-49285761	MS.gene08073:CDS
AGAAGTAACGGCTCTCAACT+TGG	0.482912	3.3:+49285689	None:intergenic
TGACATCCGAATGAATTTGA+AGG	0.495230	3.3:-49286090	MS.gene08073:CDS
CGAAACTGAACCGCAGCACC+AGG	0.498813	3.3:+49286200	None:intergenic
TTGGAATATACCTTTCCTGC+AGG	0.500426	3.3:+49285708	None:intergenic
AAGGATGTTAAGACTCTTCG+AGG	0.505057	3.3:-49285909	MS.gene08073:CDS
GATACCAAAATTCACATTCT+GGG	0.511425	3.3:-49285789	MS.gene08073:CDS
CTAATTATGGGATCACCTGC+AGG	0.512370	3.3:-49285723	MS.gene08073:CDS
CTGAAACCTCATGAGATGCC+TGG	0.516679	3.3:-49286218	MS.gene08073:CDS
TGCAAACATCAAGATTGCTA+GGG	0.524357	3.3:-49285760	MS.gene08073:CDS
TATGGGATCACCTGCAGGAA+AGG	0.544799	3.3:-49285718	MS.gene08073:CDS
CAGATAATCTTCCAATAGCA+CGG	0.551311	3.3:+49285874	None:intergenic
GACATCCGAATGAATTTGAA+GGG	0.559638	3.3:-49286089	MS.gene08073:CDS
ACCTCTCAAGAAAGTTTGGA+TGG	0.563023	3.3:-49286147	MS.gene08073:CDS
TCATGAGATGCCTGGTGCTG+CGG	0.567566	3.3:-49286210	MS.gene08073:CDS
TCCATCCAAACTTTCTTGAG+AGG	0.572045	3.3:+49286146	None:intergenic
GGAAGATTATCTGGAAAAGG+TGG	0.573573	3.3:-49285864	MS.gene08073:CDS
TTCTGCCATCCTAGAAGTAA+CGG	0.576020	3.3:+49285677	None:intergenic
GAGAGCCGTTACTTCTAGGA+TGG	0.578516	3.3:-49285682	MS.gene08073:CDS
TTACTTCTAGGATGGCAGAA+AGG	0.584575	3.3:-49285674	MS.gene08073:CDS
CACAATGGCATCATCTACAA+TGG	0.590257	3.3:-49286300	None:intergenic
AGATAATCTTCCAATAGCAC+GGG	0.591480	3.3:+49285875	None:intergenic
TGCTGCGGTTCAGTTTCGCA+AGG	0.601400	3.3:-49286195	MS.gene08073:CDS
GGCATCTCATGAGGTTTCAG+AGG	0.608176	3.3:+49286221	None:intergenic
CTTAACATCCTTGATCTCAA+AGG	0.609805	3.3:+49285920	None:intergenic
GCAGCACCAGGCATCTCATG+AGG	0.646562	3.3:+49286212	None:intergenic
TTGAGAGGTGTATAACGATG+TGG	0.685518	3.3:+49286161	None:intergenic
GAAAAGTTGAGTTGAAGACA+AGG	0.702510	3.3:-49286064	MS.gene08073:CDS
AGAGGTGTATAACGATGTGG+TGG	0.702702	3.3:+49286164	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TCTCTTTGTTACCTAATTAT+GGG	-	chr3.3:49286229-49286248	MS.gene08073:CDS	25.0%
!	TTCTCTTTGTTACCTAATTA+TGG	-	chr3.3:49286228-49286247	MS.gene08073:CDS	25.0%
!!	AATTTTCATCTGCTCAAATA+CGG	+	chr3.3:49285855-49285874	None:intergenic	25.0%
	ATTGGAAGATTATCTGGAAA+AGG	-	chr3.3:49286097-49286116	MS.gene08073:CDS	30.0%
	GATACCAAAATTCACATTCT+GGG	-	chr3.3:49286175-49286194	MS.gene08073:CDS	30.0%
	TGATACCAAAATTCACATTC+TGG	-	chr3.3:49286174-49286193	MS.gene08073:CDS	30.0%
	TGCATTCTCAATTGTAAACT+TGG	+	chr3.3:49286131-49286150	None:intergenic	30.0%
	TTTGTTCATGCTTTCATGTT+GGG	-	chr3.3:49285965-49285984	MS.gene08073:CDS	30.0%
!	ATTTTCATCTGCTCAAATAC+GGG	+	chr3.3:49285854-49285873	None:intergenic	30.0%
!	GCAAAACTTTTTGTTCAGAT+GGG	+	chr3.3:49285703-49285722	None:intergenic	30.0%
!	TTTTGTTCATGCTTTCATGT+TGG	-	chr3.3:49285964-49285983	MS.gene08073:CDS	30.0%
	AGATAATCTTCCAATAGCAC+GGG	+	chr3.3:49286092-49286111	None:intergenic	35.0%
	ATACACCTCTCAAGAAAGTT+TGG	-	chr3.3:49285813-49285832	MS.gene08073:CDS	35.0%
	CAGATAATCTTCCAATAGCA+CGG	+	chr3.3:49286093-49286112	None:intergenic	35.0%
	CTTAACATCCTTGATCTCAA+AGG	+	chr3.3:49286047-49286066	None:intergenic	35.0%
	GACATCCGAATGAATTTGAA+GGG	-	chr3.3:49285875-49285894	MS.gene08073:CDS	35.0%
	TGACATCCGAATGAATTTGA+AGG	-	chr3.3:49285874-49285893	MS.gene08073:CDS	35.0%
	TGCAAACATCAAGATTGCTA+GGG	-	chr3.3:49286204-49286223	MS.gene08073:CDS	35.0%
	TTCGACCCTTCAAATTCATT+CGG	+	chr3.3:49285883-49285902	None:intergenic	35.0%
	TTGCAAACATCAAGATTGCT+AGG	-	chr3.3:49286203-49286222	MS.gene08073:CDS	35.0%
!	AGATCCCAGAATGTGAATTT+TGG	+	chr3.3:49286182-49286201	None:intergenic	35.0%
!	CAAAATCAGCACATTTTTGC+AGG	+	chr3.3:49285949-49285968	None:intergenic	35.0%
!	GGCAAAACTTTTTGTTCAGA+TGG	+	chr3.3:49285704-49285723	None:intergenic	35.0%
!!	GAAAAGTTGAGTTGAAGACA+AGG	-	chr3.3:49285900-49285919	MS.gene08073:CDS	35.0%
	AAGGATGTTAAGACTCTTCG+AGG	-	chr3.3:49286055-49286074	MS.gene08073:CDS	40.0%
	ACCTCTCAAGAAAGTTTGGA+TGG	-	chr3.3:49285817-49285836	MS.gene08073:CDS	40.0%
	GGAAGATTATCTGGAAAAGG+TGG	-	chr3.3:49286100-49286119	MS.gene08073:CDS	40.0%
	TCCATCCAAACTTTCTTGAG+AGG	+	chr3.3:49285821-49285840	None:intergenic	40.0%
	TTCTGCCATCCTAGAAGTAA+CGG	+	chr3.3:49286290-49286309	None:intergenic	40.0%
	TTGGAATATACCTTTCCTGC+AGG	+	chr3.3:49286259-49286278	None:intergenic	40.0%
!	CGTGCTATTGGAAGATTATC+TGG	-	chr3.3:49286091-49286110	MS.gene08073:CDS	40.0%
!	TGCTATTGCTATTCTGCGTT+TGG	-	chr3.3:49286003-49286022	MS.gene08073:CDS	40.0%
!	TTGAGAGGTGTATAACGATG+TGG	+	chr3.3:49285806-49285825	None:intergenic	40.0%
!	TTTTGCAGGTTACTGATGTC+AGG	+	chr3.3:49285935-49285954	None:intergenic	40.0%
!!	TGTTGAGTCCTTTGAGATCA+AGG	-	chr3.3:49286036-49286055	MS.gene08073:CDS	40.0%
!!	TTACTTCTAGGATGGCAGAA+AGG	-	chr3.3:49286290-49286309	MS.gene08073:CDS	40.0%
	AGAAGTAACGGCTCTCAACT+TGG	+	chr3.3:49286278-49286297	None:intergenic	45.0%
	AGTTGAGAGCCGTTACTTCT+AGG	-	chr3.3:49286278-49286297	MS.gene08073:CDS	45.0%
	CTAATTATGGGATCACCTGC+AGG	-	chr3.3:49286241-49286260	MS.gene08073:CDS	45.0%
	CTGCAGGTGATCCCATAATT+AGG	+	chr3.3:49286243-49286262	None:intergenic	45.0%
	GGTTTCAGAGGCTCAAACTT+TGG	+	chr3.3:49285734-49285753	None:intergenic	45.0%
!	AGAGGCTCAAACTTTGGCTT+TGG	+	chr3.3:49285728-49285747	None:intergenic	45.0%
!	AGAGGTGTATAACGATGTGG+TGG	+	chr3.3:49285803-49285822	None:intergenic	45.0%
	CTGAAACCTCATGAGATGCC+TGG	-	chr3.3:49285746-49285765	MS.gene08073:CDS	50.0%
	GATCACTTGTCCCGTGCTAT+TGG	-	chr3.3:49286079-49286098	MS.gene08073:CDS	50.0%
	GGCATCTCATGAGGTTTCAG+AGG	+	chr3.3:49285746-49285765	None:intergenic	50.0%
	TATGGGATCACCTGCAGGAA+AGG	-	chr3.3:49286246-49286265	MS.gene08073:CDS	50.0%
!	GAGAGCCGTTACTTCTAGGA+TGG	-	chr3.3:49286282-49286301	MS.gene08073:CDS	50.0%
!!	GGCTCAAACTTTGGCTTTGG+AGG	+	chr3.3:49285725-49285744	None:intergenic	50.0%
	TGCTGCGGTTCAGTTTCGCA+AGG	-	chr3.3:49285769-49285788	MS.gene08073:CDS	55.0%
!	TCATGAGATGCCTGGTGCTG+CGG	-	chr3.3:49285754-49285773	MS.gene08073:CDS	55.0%
	CGAAACTGAACCGCAGCACC+AGG	+	chr3.3:49285767-49285786	None:intergenic	60.0%
	GCAGCACCAGGCATCTCATG+AGG	+	chr3.3:49285755-49285774	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	49285668	49286318	49285668	ID=MS.gene08073
chr3.3	mRNA	49285668	49286318	49285668	ID=MS.gene08073.t1;Parent=MS.gene08073
chr3.3	exon	49285668	49286318	49285668	ID=MS.gene08073.t1.exon1;Parent=MS.gene08073.t1
chr3.3	CDS	49285668	49286318	49285668	ID=cds.MS.gene08073.t1;Parent=MS.gene08073.t1

Gene Sequence

>MS.gene08073

ATGGCATCATCTACAATGGAAGTTGACAATGTCCCATCTGAACAAAAAGTTTTGCCTCCAAAGCCAAAGTTTGAGCCTCTGAAACCTCATGAGATGCCTGGTGCTGCGGTTCAGTTTCGCAAGGTATCAGTTCCACCACATCGTTATACACCTCTCAAGAAAGTTTGGATGGACATCTACACTCCCGTATTTGAGCAGATGAAAATTGACATCCGAATGAATTTGAAGGGTCGAAAAGTTGAGTTGAAGACAAGGCATGATACACCTGACATCAGTAACCTGCAAAAATGTGCTGATTTTGTTCATGCTTTCATGTTGGGTTTTGATGTCATAGATGCTATTGCTATTCTGCGTTTGGATGAGCTCTATGTTGAGTCCTTTGAGATCAAGGATGTTAAGACTCTTCGAGGTGATCACTTGTCCCGTGCTATTGGAAGATTATCTGGAAAAGGTGGTAAAACCAAGTTTACAATTGAGAATGCATCAAAGACAAGAATCGTGATTGCTGATACCAAAATTCACATTCTGGGATCTTTTGCAAACATCAAGATTGCTAGGGATTCTCTTTGTTACCTAATTATGGGATCACCTGCAGGAAAGGTATATTCCAAGTTGAGAGCCGTTACTTCTAGGATGGCAGAAAGGTTTTGA

Protein sequence

>MS.gene08073.t1

MASSTMEVDNVPSEQKVLPPKPKFEPLKPHEMPGAAVQFRKVSVPPHRYTPLKKVWMDIYTPVFEQMKIDIRMNLKGRKVELKTRHDTPDISNLQKCADFVHAFMLGFDVIDAIAILRLDELYVESFEIKDVKTLRGDHLSRAIGRLSGKGGKTKFTIENASKTRIVIADTKIHILGSFANIKIARDSLCYLIMGSPAGKVYSKLRAVTSRMAERF