AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53789

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53789.t1 MTR_3g061130 91.080 213 4 2 55 252 1 213 5.39e-138 387
MS.gene53789.t1 MTR_5g088410 63.265 196 56 4 57 252 6 185 7.46e-70 213
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53789.t1 AT3G13230 70.000 210 48 2 57 251 4 213 1.35e-104 302
MS.gene53789.t1 AT3G13230 70.000 210 48 2 57 251 4 213 1.35e-104 302

Find 62 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGGACTTTCTTGGTGTTTC+TGG 0.078101 7.2:-70262241 None:intergenic
TTCTCTTTGTTACCTAATTA+TGG 0.104484 7.2:+70262827 MS.gene53789:CDS
AGATCCCAGAATGTGAATTT+TGG 0.244767 7.2:-70262778 None:intergenic
CTGCTGGTGATCCCATAATT+AGG 0.273715 7.2:-70262839 None:intergenic
AGTTGAGAGCTGTTACTTCT+AGG 0.289042 7.2:+70262877 MS.gene53789:CDS
CGACACCAAAATTCACATTC+TGG 0.308153 7.2:+70262773 MS.gene53789:CDS
TCTCTTTGTTACCTAATTAT+GGG 0.325990 7.2:+70262828 MS.gene53789:CDS
ATACTCCTCTCAAGAAAGTT+TGG 0.353060 7.2:+70262421 MS.gene53789:intron
GGAACGTGGGAAGTGGGAAC+TGG 0.354469 7.2:+70261494 MS.gene53789:CDS
GGCTCGAACTTTGGCTTTGG+AGG 0.363818 7.2:-70262329 None:intergenic
TGCAACTGGACGGACTTTCT+TGG 0.364578 7.2:-70262250 None:intergenic
TTGGCCCATGCTTTCATGTT+GGG 0.371205 7.2:+70262564 MS.gene53789:CDS
AGAGGCTCGAACTTTGGCTT+TGG 0.373085 7.2:-70262332 None:intergenic
GATCACTTGTCCCGTGCTAT+TGG 0.373903 7.2:+70262678 MS.gene53789:CDS
CGTGCTATTGGAAGATTATC+TGG 0.376662 7.2:+70262690 MS.gene53789:CDS
TGATATTGCTATTCTGCGTT+TGG 0.381669 7.2:+70262602 MS.gene53789:CDS
TGCATTCTCAATTGTAAACT+TGG 0.386651 7.2:-70262727 None:intergenic
GGTTTCAGAGGCTCGAACTT+TGG 0.401066 7.2:-70262338 None:intergenic
TTCGACCCTTCAAATTCATT+CGG 0.417898 7.2:-70262479 None:intergenic
AGATGATGCCATTGTGCAAC+TGG 0.428477 7.2:-70262264 None:intergenic
TTACTTCTAGGATGGCATAA+AGG 0.438927 7.2:+70262889 MS.gene53789:CDS
AGATGAAGTGTGGGAATGAA+GGG 0.441276 7.2:+70261473 None:intergenic
AATGAAGGGAACGTGGGAAG+TGG 0.447295 7.2:+70261487 None:intergenic
CACAATGGCATCATCTTCAA+TGG 0.462021 7.2:+70262271 MS.gene53789:CDS
ATTGGAAGATTATCTGGAAA+AGG 0.469137 7.2:+70262696 MS.gene53789:CDS
TTGCAAACATCAAGATTGCT+AGG 0.471818 7.2:+70262802 MS.gene53789:CDS
GAACGTGGGAAGTGGGAACT+GGG 0.478474 7.2:+70261495 MS.gene53789:CDS
TGACATCCGAATGAATTTGA+AGG 0.495230 7.2:+70262473 MS.gene53789:CDS
AAAGATGTTAAGACACTTCG+AGG 0.496903 7.2:+70262654 MS.gene53789:CDS
CGAAACTGAACCGCAGCACC+AGG 0.498813 7.2:-70262371 None:intergenic
TTTGGCCCATGCTTTCATGT+TGG 0.500499 7.2:+70262563 MS.gene53789:CDS
CTCAACTCAACTGCCTGTGC+CGG 0.507612 7.2:+70262189 MS.gene53789:CDS
CTGAAACCTCATGAGATGCC+TGG 0.516679 7.2:+70262353 MS.gene53789:CDS
AGAAGTAACAGCTCTCAACT+TGG 0.520788 7.2:-70262874 None:intergenic
GACACCAAAATTCACATTCT+GGG 0.523448 7.2:+70262774 MS.gene53789:CDS
TGCAAACATCAAGATTGCTA+GGG 0.524357 7.2:+70262803 MS.gene53789:CDS
AACGTGGGAAGTGGGAACTG+GGG 0.532901 7.2:+70261496 MS.gene53789:CDS
CAAAACCCAACATGAAAGCA+TGG 0.539567 7.2:-70262569 None:intergenic
CTAATTATGGGATCACCAGC+AGG 0.540701 7.2:+70262840 MS.gene53789:CDS
ATGAAGGGAACGTGGGAAGT+GGG 0.541092 7.2:+70261488 MS.gene53789:CDS
TATGGGATCACCAGCAGGAA+AGG 0.541703 7.2:+70262845 MS.gene53789:CDS
CAGATAATCTTCCAATAGCA+CGG 0.551311 7.2:-70262689 None:intergenic
CTTAACATCTTTGATCTCAA+AGG 0.556748 7.2:-70262643 None:intergenic
GACATCCGAATGAATTTGAA+GGG 0.559638 7.2:+70262474 MS.gene53789:CDS
TGGCGGACGAAGAGCGACTT+CGG 0.561051 7.2:-70262221 None:intergenic
GTGTGGGAATGAAGGGAACG+TGG 0.561991 7.2:+70261480 None:intergenic
TTGGAATAGACCTTTCCTGC+TGG 0.562114 7.2:-70262855 None:intergenic
TCATGAGATGCCTGGTGCTG+CGG 0.567566 7.2:+70262361 MS.gene53789:CDS
GAGAGCTGTTACTTCTAGGA+TGG 0.571608 7.2:+70262881 MS.gene53789:CDS
GGAAGATTATCTGGAAAAGG+TGG 0.573573 7.2:+70262699 MS.gene53789:CDS
AGATAATCTTCCAATAGCAC+GGG 0.591480 7.2:-70262688 None:intergenic
GACTTTCTTGGTGTTTCTGG+CGG 0.596708 7.2:-70262238 None:intergenic
TGTGGGAATGAAGGGAACGT+GGG 0.599554 7.2:+70261481 None:intergenic
TGCTGCGGTTCAGTTTCGCA+AGG 0.601400 7.2:+70262376 MS.gene53789:CDS
GGCATCTCATGAGGTTTCAG+AGG 0.608176 7.2:-70262350 None:intergenic
AAAACCCAACATGAAAGCAT+GGG 0.609021 7.2:-70262568 None:intergenic
GAAGTGGGAACTGGGGACCA+TGG 0.614966 7.2:+70261503 MS.gene53789:CDS
GAAAAGTTGAGCTGAAGACA+AGG 0.617743 7.2:+70262499 MS.gene53789:CDS
GCAGCACCAGGCATCTCATG+AGG 0.646562 7.2:-70262359 None:intergenic
AAGTCCGTCCAGTTGCACAA+TGG 0.666830 7.2:+70262256 MS.gene53789:CDS
GATGCCATTGTGCAACTGGA+CGG 0.668049 7.2:-70262260 None:intergenic
ACAGGCAGTTGAGTTGAGTG+CGG 0.675309 7.2:-70262184 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTAAAAAAAACATTTTTT+TGG - chr7.2:70261781-70261800 None:intergenic 10.0%
!! CTTATAAGGTAAAAAAATAT+TGG - chr7.2:70261810-70261829 None:intergenic 15.0%
!! TTATTTGTATAAGCTTATAT+TGG + chr7.2:70261985-70262004 MS.gene53789:intron 15.0%
!!! AAAAACATTTTTTTGGTATA+AGG - chr7.2:70261774-70261793 None:intergenic 15.0%
!!! ATTTTAAAGAGTATATTACA+TGG + chr7.2:70261582-70261601 MS.gene53789:intron 15.0%
!!! TAAGGAAAATAGTTTAATTT+TGG - chr7.2:70261756-70261775 None:intergenic 15.0%
!! TTCAAAGCAGATTTAATTAT+AGG + chr7.2:70261533-70261552 MS.gene53789:intron 20.0%
!! TTTACTGTTTATCATATGTT+TGG + chr7.2:70261879-70261898 MS.gene53789:intron 20.0%
!!! AATTTTCATCTGTTCATATA+CGG - chr7.2:70262454-70262473 None:intergenic 20.0%
! TCTCTTTGTTACCTAATTAT+GGG + chr7.2:70262828-70262847 MS.gene53789:CDS 25.0%
! TTCTCTTTGTTACCTAATTA+TGG + chr7.2:70262827-70262846 MS.gene53789:CDS 25.0%
! TTTAGTCAAATGAGCTTATA+AGG - chr7.2:70261922-70261941 None:intergenic 25.0%
!! ATTTTCATCTGTTCATATAC+GGG - chr7.2:70262453-70262472 None:intergenic 25.0%
AAAAAACGGATGAGCTTATA+AGG - chr7.2:70261824-70261843 None:intergenic 30.0%
ATTGGAAGATTATCTGGAAA+AGG + chr7.2:70262696-70262715 MS.gene53789:CDS 30.0%
CTTAACATCTTTGATCTCAA+AGG - chr7.2:70262646-70262665 None:intergenic 30.0%
TGCATTCTCAATTGTAAACT+TGG - chr7.2:70262730-70262749 None:intergenic 30.0%
TGCTCGAAGAAGAAAAAAAA+CGG - chr7.2:70261838-70261857 None:intergenic 30.0%
! GCAAAACTTTTTGTTCAGAT+AGG - chr7.2:70262310-70262329 None:intergenic 30.0%
AAAGATGTTAAGACACTTCG+AGG + chr7.2:70262654-70262673 MS.gene53789:CDS 35.0%
AGATAATCTTCCAATAGCAC+GGG - chr7.2:70262691-70262710 None:intergenic 35.0%
AGGGGTAAAATTGAGATAAG+AGG - chr7.2:70262043-70262062 None:intergenic 35.0%
ATACTCCTCTCAAGAAAGTT+TGG + chr7.2:70262421-70262440 MS.gene53789:intron 35.0%
CAGATAATCTTCCAATAGCA+CGG - chr7.2:70262692-70262711 None:intergenic 35.0%
GACACCAAAATTCACATTCT+GGG + chr7.2:70262774-70262793 MS.gene53789:CDS 35.0%
GACATCCGAATGAATTTGAA+GGG + chr7.2:70262474-70262493 MS.gene53789:CDS 35.0%
GGGGTAAAATTGAGATAAGA+GGG - chr7.2:70262042-70262061 None:intergenic 35.0%
TGACATCCGAATGAATTTGA+AGG + chr7.2:70262473-70262492 MS.gene53789:CDS 35.0%
TGCAAACATCAAGATTGCTA+GGG + chr7.2:70262803-70262822 MS.gene53789:CDS 35.0%
TGCCATGTAATATACTCTTG+AGG - chr7.2:70261678-70261697 None:intergenic 35.0%
TTCGACCCTTCAAATTCATT+CGG - chr7.2:70262482-70262501 None:intergenic 35.0%
TTGCAAACATCAAGATTGCT+AGG + chr7.2:70262802-70262821 MS.gene53789:CDS 35.0%
TTTCTCCAGTTAAGATTGCA+GGG - chr7.2:70262062-70262081 None:intergenic 35.0%
! AAAACCCAACATGAAAGCAT+GGG - chr7.2:70262571-70262590 None:intergenic 35.0%
! AGATCCCAGAATGTGAATTT+TGG - chr7.2:70262781-70262800 None:intergenic 35.0%
! CTCCTCAAGAGTATATTACA+TGG + chr7.2:70261673-70261692 MS.gene53789:intron 35.0%
! TGATATTGCTATTCTGCGTT+TGG + chr7.2:70262602-70262621 MS.gene53789:CDS 35.0%
! TTTTCTCCAGTTAAGATTGC+AGG - chr7.2:70262063-70262082 None:intergenic 35.0%
!! CAGAATGTGAATTTTGGTGT+CGG - chr7.2:70262775-70262794 None:intergenic 35.0%
AGAAGTAACAGCTCTCAACT+TGG - chr7.2:70262877-70262896 None:intergenic 40.0%
ATTTCATGTTTCTCTCTCCC+CGG + chr7.2:70262119-70262138 MS.gene53789:intron 40.0%
CAAAACCCAACATGAAAGCA+TGG - chr7.2:70262572-70262591 None:intergenic 40.0%
CACAATGGCATCATCTTCAA+TGG + chr7.2:70262271-70262290 MS.gene53789:CDS 40.0%
CGACACCAAAATTCACATTC+TGG + chr7.2:70262773-70262792 MS.gene53789:CDS 40.0%
CTTCTTCGAGCACTCAAAAT+GGG + chr7.2:70261846-70261865 MS.gene53789:intron 40.0%
GAAAAGTTGAGCTGAAGACA+AGG + chr7.2:70262499-70262518 MS.gene53789:CDS 40.0%
GCAGAATGCTGCAATACTAT+TGG + chr7.2:70261604-70261623 MS.gene53789:intron 40.0%
GGAAGATTATCTGGAAAAGG+TGG + chr7.2:70262699-70262718 MS.gene53789:CDS 40.0%
GTGTACCAAACTTTCTTGAG+AGG - chr7.2:70262429-70262448 None:intergenic 40.0%
TCTTCTTCGAGCACTCAAAA+TGG + chr7.2:70261845-70261864 MS.gene53789:intron 40.0%
TTCTCCAGTTAAGATTGCAG+GGG - chr7.2:70262061-70262080 None:intergenic 40.0%
TTTACCCCTGCAATCTTAAC+TGG + chr7.2:70262054-70262073 MS.gene53789:intron 40.0%
! AGTTGAGAGCTGTTACTTCT+AGG + chr7.2:70262877-70262896 MS.gene53789:CDS 40.0%
! CCAAATCAGCACATTTTTGC+AGG - chr7.2:70262548-70262567 None:intergenic 40.0%
! CGTGCTATTGGAAGATTATC+TGG + chr7.2:70262690-70262709 MS.gene53789:CDS 40.0%
! TTGATGCACTGACAGTGTAA+AGG - chr7.2:70261646-70261665 None:intergenic 40.0%
! TTTTGCAGGTTACTGATGTC+AGG - chr7.2:70262534-70262553 None:intergenic 40.0%
!! CCTGCAAAAATGTGCTGATT+TGG + chr7.2:70262545-70262564 MS.gene53789:CDS 40.0%
!! TGAGAGGAGTATTAACGATG+TGG - chr7.2:70262413-70262432 None:intergenic 40.0%
AGATGATGCCATTGTGCAAC+TGG - chr7.2:70262267-70262286 None:intergenic 45.0%
CTAATTATGGGATCACCAGC+AGG + chr7.2:70262840-70262859 MS.gene53789:CDS 45.0%
CTGCCACAGAACTTCAAAAC+CGG - chr7.2:70262141-70262160 None:intergenic 45.0%
CTGCTGGTGATCCCATAATT+AGG - chr7.2:70262842-70262861 None:intergenic 45.0%
TGCCACAGAACTTCAAAACC+GGG - chr7.2:70262140-70262159 None:intergenic 45.0%
TTGGAATAGACCTTTCCTGC+TGG - chr7.2:70262858-70262877 None:intergenic 45.0%
TTGGCCCATGCTTTCATGTT+GGG + chr7.2:70262564-70262583 MS.gene53789:CDS 45.0%
TTTGGCCCATGCTTTCATGT+TGG + chr7.2:70262563-70262582 MS.gene53789:CDS 45.0%
! CTGCTTTGAACTACCTACCA+TGG - chr7.2:70261523-70261542 None:intergenic 45.0%
! GAGAGCTGTTACTTCTAGGA+TGG + chr7.2:70262881-70262900 MS.gene53789:CDS 45.0%
! TTGGTGCACTGATCGTGTAA+AGG - chr7.2:70261737-70261756 None:intergenic 45.0%
!! ACGGACTTTCTTGGTGTTTC+TGG - chr7.2:70262244-70262263 None:intergenic 45.0%
!! GACTTTCTTGGTGTTTCTGG+CGG - chr7.2:70262241-70262260 None:intergenic 45.0%
!! GAGGAGTATTAACGATGTGG+TGG - chr7.2:70262410-70262429 None:intergenic 45.0%
AAGTCCGTCCAGTTGCACAA+TGG + chr7.2:70262256-70262275 MS.gene53789:CDS 50.0%
ATGAAGGGAACGTGGGAAGT+GGG + chr7.2:70261488-70261507 MS.gene53789:CDS 50.0%
CTGAAACCTCATGAGATGCC+TGG + chr7.2:70262353-70262372 MS.gene53789:CDS 50.0%
GATCACTTGTCCCGTGCTAT+TGG + chr7.2:70262678-70262697 MS.gene53789:CDS 50.0%
GATGCCATTGTGCAACTGGA+CGG - chr7.2:70262263-70262282 None:intergenic 50.0%
GCCACAGAACTTCAAAACCG+GGG - chr7.2:70262139-70262158 None:intergenic 50.0%
GGCATCTCATGAGGTTTCAG+AGG - chr7.2:70262353-70262372 None:intergenic 50.0%
GGTTTCAGAGGCTCGAACTT+TGG - chr7.2:70262341-70262360 None:intergenic 50.0%
TATGGGATCACCAGCAGGAA+AGG + chr7.2:70262845-70262864 MS.gene53789:CDS 50.0%
! AGAGGCTCGAACTTTGGCTT+TGG - chr7.2:70262335-70262354 None:intergenic 50.0%
! GCGACTTCGGCTTCTTTTTC+CGG - chr7.2:70262211-70262230 None:intergenic 50.0%
! TGCAACTGGACGGACTTTCT+TGG - chr7.2:70262253-70262272 None:intergenic 50.0%
!! ACAGGCAGTTGAGTTGAGTG+CGG - chr7.2:70262187-70262206 None:intergenic 50.0%
!!! TCCCCGGTTTTGAAGTTCTG+TGG + chr7.2:70262135-70262154 MS.gene53789:intron 50.0%
AACGTGGGAAGTGGGAACTG+GGG + chr7.2:70261496-70261515 MS.gene53789:CDS 55.0%
CTCAACTCAACTGCCTGTGC+CGG + chr7.2:70262189-70262208 MS.gene53789:CDS 55.0%
GAACGTGGGAAGTGGGAACT+GGG + chr7.2:70261495-70261514 MS.gene53789:CDS 55.0%
TGCTGCGGTTCAGTTTCGCA+AGG + chr7.2:70262376-70262395 MS.gene53789:CDS 55.0%
! TCATGAGATGCCTGGTGCTG+CGG + chr7.2:70262361-70262380 MS.gene53789:CDS 55.0%
! TCGGCTTCTTTTTCCGGCAC+AGG - chr7.2:70262205-70262224 None:intergenic 55.0%
!! GGCTCGAACTTTGGCTTTGG+AGG - chr7.2:70262332-70262351 None:intergenic 55.0%
CGAAACTGAACCGCAGCACC+AGG - chr7.2:70262374-70262393 None:intergenic 60.0%
GAAGTGGGAACTGGGGACCA+TGG + chr7.2:70261503-70261522 MS.gene53789:CDS 60.0%
GCAGCACCAGGCATCTCATG+AGG - chr7.2:70262362-70262381 None:intergenic 60.0%
GGAACGTGGGAAGTGGGAAC+TGG + chr7.2:70261494-70261513 MS.gene53789:CDS 60.0%
TGGGAACTGGGGACCATGGT+AGG + chr7.2:70261507-70261526 MS.gene53789:intron 60.0%
! TGGCGGACGAAGAGCGACTT+CGG - chr7.2:70262224-70262243 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 70261488 70262908 70261488 ID=MS.gene53789
chr7.2 mRNA 70261488 70262908 70261488 ID=MS.gene53789.t1;Parent=MS.gene53789
chr7.2 exon 70261488 70261524 70261488 ID=MS.gene53789.t1.exon1;Parent=MS.gene53789.t1
chr7.2 CDS 70261488 70261524 70261488 ID=cds.MS.gene53789.t1;Parent=MS.gene53789.t1
chr7.2 exon 70262150 70262397 70262150 ID=MS.gene53789.t1.exon2;Parent=MS.gene53789.t1
chr7.2 CDS 70262150 70262397 70262150 ID=cds.MS.gene53789.t1;Parent=MS.gene53789.t1
chr7.2 exon 70262435 70262908 70262435 ID=MS.gene53789.t1.exon3;Parent=MS.gene53789.t1
chr7.2 CDS 70262435 70262908 70262435 ID=cds.MS.gene53789.t1;Parent=MS.gene53789.t1
Gene Sequence

>MS.gene53789

ATGAAGGGAACGTGGGAAGTGGGAACTGGGGACCATGGTAGGTAGTTCAAAGCAGATTTAATTATAGGTTTATTTTTTTGAAATAAAAAAACATATTTTAAAGAGTATATTACATGGCAGAATGCTGCAATACTATTGGATGAGTGTGTAAAGAGCCTTTACACTGTCAGTGCATCAAAATTAAACTCCTCAAGAGTATATTACATGGCAGAATGCTGCAATACTATTGGATGAGTGTGTAAAGAGCCTTTACACGATCAGTGCACCAAAATTAAACTATTTTCCTTATACCAAAAAAATGTTTTTTTTACATGAAAAGCCAATATTTTTTTACCTTATAAGCTCATCCGTTTTTTTTCTTCTTCGAGCACTCAAAATGGGTGATTTATCATTTACTGTTTATCATATGTTTGGTAAGACATATTTTTGTACCTTATAAGCTCATTTGACTAAAAATTATTCGTTTAATAATGATCATTTTCTTATGAGTCTATAGTTTATTTGTATAAGCTTATATTGGTACAAGCTAATTCAAATAGTTTATATCTCATCCCTCTTATCTCAATTTTACCCCTGCAATCTTAACTGGAGAAAATAATTATTAATCAAATGCAAATATATTTTTTATGTTATTTCATGTTTCTCTCTCCCCGGTTTTGAAGTTCTGTGGCAGACACAACTTAGAGTTGCTCTTTGCCGCACTCAACTCAACTGCCTGTGCCGGAAAAAGAAGCCGAAGTCGCTCTTCGTCCGCCAGAAACACCAAGAAAGTCCGTCCAGTTGCACAATGGCATCATCTTCAATGGAAGTTGACAATGTCCTATCTGAACAAAAAGTTTTGCCTCCAAAGCCAAAGTTCGAGCCTCTGAAACCTCATGAGATGCCTGGTGCTGCGGTTCAGTTTCGCAAGGTATCAGTTCCACCACATCGTTAATACTCCTCTCAAGAAAGTTTGGTACACTCCCGTATATGAACAGATGAAAATTGACATCCGAATGAATTTGAAGGGTCGAAAAGTTGAGCTGAAGACAAGGCACGATACACCTGACATCAGTAACCTGCAAAAATGTGCTGATTTGGCCCATGCTTTCATGTTGGGTTTTGATGTCATTGATGATATTGCTATTCTGCGTTTGGATGAGCTCTATGTTGAGTCCTTTGAGATCAAAGATGTTAAGACACTTCGAGGTGATCACTTGTCCCGTGCTATTGGAAGATTATCTGGAAAAGGTGGTAAAACCAAGTTTACAATTGAGAATGCATCAAAGACACGAATTGTGATTGCCGACACCAAAATTCACATTCTGGGATCTTTTGCAAACATCAAGATTGCTAGGGATTCTCTTTGTTACCTAATTATGGGATCACCAGCAGGAAAGGTCTATTCCAAGTTGAGAGCTGTTACTTCTAGGATGGCATAA
Protein sequence

>MS.gene53789.t1

MKGTWEVGTGDHVLWQTQLRVALCRTQLNCLCRKKKPKSLFVRQKHQESPSSCTMASSSMEVDNVLSEQKVLPPKPKFEPLKPHEMPGAAVQFRKKVWYTPVYEQMKIDIRMNLKGRKVELKTRHDTPDISNLQKCADLAHAFMLGFDVIDDIAILRLDELYVESFEIKDVKTLRGDHLSRAIGRLSGKGGKTKFTIENASKTRIVIADTKIHILGSFANIKIARDSLCYLIMGSPAGKVYSKLRAVTSRMA