AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene005221

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005221.t1 MTR_1g082760 98.976 293 3 0 1 293 1 293 0.0 607
MS.gene005221.t1 MTR_7g113650 86.301 292 40 0 1 292 1 292 0.0 544
MS.gene005221.t1 MTR_4g131740 71.088 294 84 1 1 293 1 294 1.53e-158 443
MS.gene005221.t1 MTR_6g023590 32.075 265 163 7 32 288 5 260 2.94e-31 117
MS.gene005221.t1 MTR_6g023610 33.962 265 148 8 39 290 12 262 4.17e-30 114
MS.gene005221.t1 MTR_6g023990 32.061 262 153 8 39 288 13 261 4.30e-30 114
MS.gene005221.t1 MTR_6g023600 33.962 265 158 7 32 288 5 260 1.12e-29 113
MS.gene005221.t1 MTR_6g023950 33.208 265 160 7 32 288 5 260 3.12e-29 112
MS.gene005221.t1 MTR_4g052320 34.231 260 155 7 39 292 7 256 3.58e-28 109
MS.gene005221.t1 MTR_3g085740 30.469 256 166 6 39 291 74 320 5.68e-28 110
MS.gene005221.t1 MTR_7g063270 32.031 256 158 7 40 288 16 262 1.11e-27 108
MS.gene005221.t1 MTR_4g052300 33.463 257 157 6 39 291 7 253 1.79e-27 107
MS.gene005221.t1 MTR_6g023910 32.836 268 149 10 35 287 8 259 6.76e-27 106
MS.gene005221.t1 MTR_4g132850 28.854 253 166 6 39 288 6 247 9.64e-26 102
MS.gene005221.t1 MTR_4g052350 32.685 257 159 6 39 291 13 259 1.20e-25 103
MS.gene005221.t1 MTR_3g020670 31.203 266 161 6 36 288 12 268 9.73e-25 100
MS.gene005221.t1 MTR_6g023600 33.208 265 154 8 32 288 5 254 9.74e-25 100
MS.gene005221.t1 MTR_7g063260 29.961 257 163 8 40 288 16 263 5.22e-24 98.6
MS.gene005221.t1 MTR_4g052340 30.233 258 166 5 39 292 13 260 5.45e-24 98.6
MS.gene005221.t1 MTR_4g078630 29.811 265 158 6 39 288 18 269 1.27e-23 97.8
MS.gene005221.t1 MTR_7g071680 27.957 279 158 9 39 288 28 292 2.40e-22 94.7
MS.gene005221.t1 MTR_8g442750 29.699 266 166 7 34 288 9 264 5.04e-22 94.0
MS.gene005221.t1 MTR_4g058810 26.766 269 179 6 35 293 6 266 4.72e-21 90.5
MS.gene005221.t1 MTR_7g105590 32.143 196 119 4 39 230 7 192 6.55e-21 88.6
MS.gene005221.t1 MTR_7g094510 30.385 260 162 8 40 290 6 255 1.99e-20 88.6
MS.gene005221.t1 MTR_4g058840 26.766 269 179 6 35 293 6 266 2.07e-20 89.0
MS.gene005221.t1 MTR_4g058820 26.766 269 179 6 35 293 6 266 2.07e-20 89.0
MS.gene005221.t1 MTR_4g064887 28.516 256 172 6 40 292 15 262 2.32e-20 88.6
MS.gene005221.t1 MTR_1196s0010 30.556 252 153 8 43 288 277 512 4.10e-20 90.5
MS.gene005221.t1 MTR_7g083870 29.278 263 162 7 39 288 34 285 5.73e-20 88.2
MS.gene005221.t1 MTR_7g063320 27.863 262 168 7 40 291 15 265 8.20e-19 84.3
MS.gene005221.t1 MTR_7g110140 28.897 263 158 6 39 288 5 251 1.90e-18 83.2
MS.gene005221.t1 MTR_2g090165 28.826 281 144 12 39 292 5 256 2.23e-18 83.2
MS.gene005221.t1 MTR_7g105460 35.176 199 117 4 39 234 12 201 3.00e-18 82.8
MS.gene005221.t1 MTR_7g063390 25.769 260 176 7 40 291 16 266 7.92e-18 81.6
MS.gene005221.t1 MTR_7g105580 29.741 232 128 6 37 264 5 205 9.89e-18 81.3
MS.gene005221.t1 MTR_7g094520 27.451 255 176 6 40 290 11 260 1.34e-17 80.9
MS.gene005221.t1 MTR_7g110130 27.341 267 167 8 39 292 5 257 2.39e-17 80.1
MS.gene005221.t1 MTR_4g064883 26.394 269 178 9 34 293 5 262 2.59e-17 80.1
MS.gene005221.t1 MTR_7g107670 26.136 264 168 7 39 288 32 282 1.23e-16 79.0
MS.gene005221.t1 MTR_8g096620 28.283 198 133 4 34 230 40 229 2.23e-16 78.6
MS.gene005221.t1 MTR_7g063270 26.772 254 125 6 40 288 16 213 1.05e-15 74.7
MS.gene005221.t1 MTR_1g068955 27.305 282 177 6 20 293 6 267 1.56e-15 75.5
MS.gene005221.t1 MTR_3g068065 27.027 259 174 8 40 293 17 265 2.16e-15 75.1
MS.gene005221.t1 MTR_6g035395 27.612 268 142 8 39 288 4 237 2.68e-15 74.3
MS.gene005221.t1 MTR_7g110160 28.470 281 127 13 39 292 5 238 4.96e-15 73.6
MS.gene005221.t1 MTR_3g068155 27.519 258 174 7 40 293 19 267 6.21e-15 73.6
MS.gene005221.t1 MTR_1g051165 26.238 202 143 4 33 228 7 208 7.78e-15 73.6
MS.gene005221.t1 MTR_8g096580 27.111 225 150 6 37 258 42 255 8.80e-15 73.9
MS.gene005221.t1 MTR_3g068175 26.357 258 177 6 40 293 17 265 1.50e-14 72.4
MS.gene005221.t1 MTR_3g087850 30.256 195 110 6 44 230 30 206 3.13e-14 72.0
MS.gene005221.t1 MTR_7g063360 25.188 266 170 8 40 291 16 266 3.82e-14 71.2
MS.gene005221.t1 MTR_5g008850 26.471 238 158 7 33 261 38 267 3.50e-13 69.3
MS.gene005221.t1 MTR_4g064887 27.411 197 134 4 40 234 15 204 2.00e-11 62.8
MS.gene005221.t1 MTR_4g084800 31.618 136 83 3 39 173 12 138 4.50e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005221.t1 AT1G54870 79.152 283 59 0 11 293 55 337 3.89e-174 484
MS.gene005221.t1 AT1G54870 79.152 283 59 0 11 293 53 335 6.19e-174 484
MS.gene005221.t1 AT3G05260 71.331 293 80 2 1 293 1 289 2.36e-163 456
MS.gene005221.t1 AT3G51680 28.664 307 179 10 8 288 1 293 1.39e-24 100
MS.gene005221.t1 AT4G05530 28.958 259 170 5 33 288 2 249 6.69e-24 98.2
MS.gene005221.t1 AT3G26770 30.189 265 161 7 37 288 38 291 3.35e-23 97.1
MS.gene005221.t1 AT1G24360 27.509 269 182 6 28 291 59 319 3.76e-23 97.4
MS.gene005221.t1 AT5G06060 31.395 258 164 7 40 293 9 257 1.55e-22 94.7
MS.gene005221.t1 AT2G47140 31.179 263 152 7 39 288 5 251 2.42e-22 94.0
MS.gene005221.t1 AT1G52340 30.451 266 160 8 39 288 17 273 2.62e-22 94.4
MS.gene005221.t1 AT1G63380 26.374 273 190 6 28 293 5 273 5.14e-22 93.6
MS.gene005221.t1 AT1G63380 26.692 266 184 6 35 293 14 275 7.50e-22 93.2
MS.gene005221.t1 AT1G63380 26.692 266 184 6 35 293 10 271 7.69e-22 92.8
MS.gene005221.t1 AT1G63380 26.692 266 184 6 35 293 17 278 8.36e-22 93.2
MS.gene005221.t1 AT1G62610 26.007 273 191 6 28 293 2 270 7.60e-21 90.1
MS.gene005221.t1 AT1G62610 26.316 266 185 6 35 293 10 271 8.90e-21 90.1
MS.gene005221.t1 AT1G62610 26.316 266 185 6 35 293 8 269 8.98e-21 90.1
MS.gene005221.t1 AT1G62610 26.316 266 185 6 35 293 14 275 9.77e-21 90.1
MS.gene005221.t1 AT2G29290 29.845 258 168 7 40 293 7 255 1.23e-20 89.4
MS.gene005221.t1 AT2G47120 30.798 263 159 7 38 291 5 253 2.33e-20 88.6
MS.gene005221.t1 AT2G29350 30.233 258 166 7 40 293 15 262 2.70e-20 88.6
MS.gene005221.t1 AT2G47120 30.916 262 158 7 39 291 5 252 2.89e-20 88.2
MS.gene005221.t1 AT3G04000 28.244 262 175 7 40 291 14 272 3.19e-20 88.6
MS.gene005221.t1 AT3G55290 29.000 200 133 4 34 228 12 207 2.06e-19 86.3
MS.gene005221.t1 AT3G55290 29.000 200 133 4 34 228 11 206 2.51e-19 85.9
MS.gene005221.t1 AT3G26760 28.731 268 163 10 37 288 33 288 4.10e-19 85.9
MS.gene005221.t1 AT2G47130 30.038 263 154 8 39 288 5 250 4.39e-19 85.1
MS.gene005221.t1 AT2G29150 29.057 265 170 8 35 293 11 263 4.88e-19 85.1
MS.gene005221.t1 AT2G29260 29.502 261 165 8 40 293 68 316 5.01e-19 85.9
MS.gene005221.t1 AT3G12800 28.044 271 165 10 34 288 5 261 7.78e-19 85.1
MS.gene005221.t1 AT5G50600 26.423 246 166 6 37 280 42 274 8.47e-19 85.5
MS.gene005221.t1 AT5G50700 26.423 246 166 6 37 280 42 274 8.47e-19 85.5
MS.gene005221.t1 AT3G29260 30.682 264 151 8 39 288 5 250 9.18e-19 84.0
MS.gene005221.t1 AT5G18210 28.736 261 166 10 38 287 6 257 3.46e-18 82.4
MS.gene005221.t1 AT3G42960 29.057 265 161 5 39 288 7 259 4.16e-18 82.4
MS.gene005221.t1 AT4G13180 29.213 267 174 9 32 291 5 263 6.24e-18 82.0
MS.gene005221.t1 AT3G03980 27.239 268 177 8 35 290 9 270 8.61e-18 81.6
MS.gene005221.t1 AT3G29250 28.137 263 160 6 39 288 5 251 1.45e-17 80.9
MS.gene005221.t1 AT2G29360 27.925 265 174 7 35 293 11 264 2.25e-17 80.5
MS.gene005221.t1 AT3G29250 28.137 263 160 6 39 288 41 287 2.96e-17 80.5
MS.gene005221.t1 AT2G30670 29.658 263 162 9 40 293 7 255 5.35e-17 79.3
MS.gene005221.t1 AT1G07440 27.586 261 169 7 40 293 12 259 6.13e-17 79.3
MS.gene005221.t1 AT3G29250 28.244 262 159 6 40 288 44 289 6.59e-17 79.7
MS.gene005221.t1 AT4G03140 26.592 267 163 6 39 288 77 327 1.04e-16 79.7
MS.gene005221.t1 AT5G10050 32.292 192 114 5 44 232 9 187 1.16e-16 78.6
MS.gene005221.t1 AT3G55310 25.641 273 191 7 28 293 5 272 1.21e-16 78.6
MS.gene005221.t1 AT5G18210 28.455 246 157 9 38 272 6 243 4.37e-16 77.0
MS.gene005221.t1 AT2G29310 29.588 267 155 11 40 292 7 254 1.35e-15 75.5
MS.gene005221.t1 AT3G47350 24.427 262 171 8 30 274 34 285 1.82e-15 75.5
MS.gene005221.t1 AT2G17845 26.540 211 145 5 39 242 46 253 2.06e-15 75.5
MS.gene005221.t1 AT2G29370 26.296 270 172 8 35 293 11 264 2.63e-15 74.7
MS.gene005221.t1 AT2G29350 32.240 183 117 5 115 293 45 224 2.97e-15 73.9
MS.gene005221.t1 AT1G07450 27.308 260 173 8 39 293 7 255 5.63e-15 73.6
MS.gene005221.t1 AT5G50690 28.788 198 131 5 35 230 40 229 5.99e-15 73.9
MS.gene005221.t1 AT5G50590 28.788 198 131 5 35 230 40 229 5.99e-15 73.9
MS.gene005221.t1 AT5G50690 30.151 199 127 7 35 230 40 229 6.12e-15 73.2
MS.gene005221.t1 AT5G50590 30.151 199 127 7 35 230 40 229 6.12e-15 73.2
MS.gene005221.t1 AT3G47350 24.681 235 163 6 30 260 34 258 1.16e-14 73.6
MS.gene005221.t1 AT2G29290 30.208 192 128 5 106 293 49 238 1.48e-14 72.0
MS.gene005221.t1 AT3G46170 27.136 199 136 5 35 228 21 215 2.53e-14 72.0
MS.gene005221.t1 AT5G65205 30.000 190 117 5 44 230 10 186 2.67e-14 72.0
MS.gene005221.t1 AT2G29320 27.757 263 168 9 40 293 13 262 1.22e-12 67.0
MS.gene005221.t1 AT4G10020 26.486 185 127 4 33 215 40 217 1.41e-12 67.8
MS.gene005221.t1 AT2G29340 28.295 258 172 7 40 293 7 255 2.13e-12 66.2
MS.gene005221.t1 AT2G29310 32.961 179 101 7 125 293 84 253 4.00e-12 65.5
MS.gene005221.t1 AT2G29300 27.444 266 165 11 40 293 7 256 2.93e-11 63.2
MS.gene005221.t1 AT2G29320 32.203 177 106 7 125 293 74 244 7.24e-11 61.6

Find 72 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAAAAGATGCTGGTATTAA+TGG 0.214643 1.4:+66988719 None:intergenic
AGCTGGTAAGCAAGGGTATA+AGG 0.257323 1.4:-66988775 MS.gene005221:CDS
TATACTTGAAGTTTGTTTGA+TGG 0.274878 1.4:+66990005 None:intergenic
AATGGCTGTAGCAGCTGATT+TGG 0.283657 1.4:-66989265 MS.gene005221:CDS
GATGAGATTGTTAATGCTTA+TGG 0.332143 1.4:-66989213 MS.gene005221:CDS
TTGTTTGATGGCTTGTAGTC+AGG 0.340288 1.4:+66990017 None:intergenic
GAGGAAGAAACTGCTCAATT+TGG 0.343335 1.4:-66988695 MS.gene005221:CDS
ATGGCTGTAGCAGCTGATTT+GGG 0.347891 1.4:-66989264 MS.gene005221:CDS
TGCAACCGTAGCACCTTCTA+AGG 0.360573 1.4:+66989377 None:intergenic
AAAAGCCACAATTGCACCTT+TGG 0.391395 1.4:+66988817 None:intergenic
ACAGGAGGTGACTCTGGAAT+TGG 0.394290 1.4:-66989423 MS.gene005221:CDS
GCCTGGGAAGGAACATGTTA+TGG 0.396697 1.4:-66990063 MS.gene005221:CDS
GTTGCATTTACATATGTAAA+GGG 0.404353 1.4:-66989360 MS.gene005221:CDS
TGTTAACAAGAATATCAATA+TGG 0.406163 1.4:+66989190 None:intergenic
TAGGATGGAGCAACCTCAAT+AGG 0.420125 1.4:+66988641 None:intergenic
GAGTGGCACCTGGGCCCATA+TGG 0.425158 1.4:-66988745 MS.gene005221:CDS
TGGTATTAATGGTGTCCATA+TGG 0.436531 1.4:+66988730 None:intergenic
CAAAAGCAAGAGACACAGCC+TGG 0.437059 1.4:-66990080 MS.gene005221:CDS
GTTAATTACCATTGGGGTGA+AGG 0.451118 1.4:+66988570 None:intergenic
AAAATAAGAGGAACATTGGT+TGG 0.452767 1.4:+66988604 None:intergenic
TTGTTTGTAAGAATGGCTTC+AGG 0.454658 1.4:-66990122 None:intergenic
AGGGTCAATGGAGTGGCACC+TGG 0.463348 1.4:-66988755 MS.gene005221:CDS
GGTAAGGTAGCTGTAATAAC+AGG 0.464066 1.4:-66989441 MS.gene005221:CDS
TCTTCCTCCTTAAAAGATGC+TGG 0.464676 1.4:+66988710 None:intergenic
GTAATAACAGGAGGTGACTC+TGG 0.469701 1.4:-66989429 MS.gene005221:CDS
AAATCAGCTGCTACAGCCAT+TGG 0.473059 1.4:+66989267 None:intergenic
GGTTGCATTTACATATGTAA+AGG 0.478222 1.4:-66989361 MS.gene005221:CDS
GCTGGTAAGCAAGGGTATAA+GGG 0.491721 1.4:-66988774 MS.gene005221:CDS
AGGACACACTAGAAATGATA+AGG 0.503643 1.4:-66989317 MS.gene005221:CDS
TGCAAAGAGTGCTGATGCTA+AGG 0.507275 1.4:-66989292 MS.gene005221:CDS
AGCAAGGGTATAAGGGTCAA+TGG 0.508243 1.4:-66988767 MS.gene005221:CDS
TGTTTGATGGCTTGTAGTCA+GGG 0.510691 1.4:+66990018 None:intergenic
ATCACTTCAGCTGGTAAGCA+AGG 0.512052 1.4:-66988783 MS.gene005221:CDS
GGTATTAATGGTGTCCATAT+GGG 0.514396 1.4:+66988731 None:intergenic
TCCATAACATGTTCCTTCCC+AGG 0.521691 1.4:+66990062 None:intergenic
TCAGGTGGAACCGTGGTGAA+TGG 0.529354 1.4:-66988474 MS.gene005221:intron
GAAGAAACTGCTCAATTTGG+TGG 0.540906 1.4:-66988692 MS.gene005221:CDS
GACTTGTCCAGTAAAATAAG+AGG 0.544263 1.4:+66988592 None:intergenic
GCACTGAAGCACATGAAGGA+AGG 0.556303 1.4:-66988911 MS.gene005221:CDS
TGCTGATGCTAAGGATCCAA+TGG 0.557285 1.4:-66989283 MS.gene005221:CDS
AATGGTGTCCATATGGGCCC+AGG 0.558819 1.4:+66988737 None:intergenic
GAGGCAAGAAAGACATAGGA+TGG 0.558839 1.4:+66988626 None:intergenic
AAGAGCTGGTCAGCCTATTG+AGG 0.560279 1.4:-66988654 MS.gene005221:CDS
ATCTACCAAAGGTGCAATTG+TGG 0.562333 1.4:-66988822 MS.gene005221:CDS
TGATGAAGACAAGGATGCTA+AGG 0.565763 1.4:-66989337 MS.gene005221:CDS
TGATGAGAATTGCAAGAGAG+TGG 0.569733 1.4:-66989238 MS.gene005221:CDS
CACTGAAGCACATGAAGGAA+GGG 0.573720 1.4:-66988910 MS.gene005221:CDS
GGGCAAGTAAATTGAGGCAG+CGG 0.573945 1.4:+66990038 None:intergenic
AAGGGGACTATCACTTCAGC+TGG 0.574318 1.4:-66988792 MS.gene005221:CDS
CAAGTGCCTATGAAAAGAGC+TGG 0.576070 1.4:-66988668 MS.gene005221:CDS
AAAAGCAAGAGACACAGCCT+GGG 0.578653 1.4:-66990079 MS.gene005221:CDS
ATAAGAGGAACATTGGTTGG+AGG 0.579832 1.4:+66988607 None:intergenic
CAGTAAAATAAGAGGAACAT+TGG 0.583914 1.4:+66988600 None:intergenic
GCAAGAGACACAGCCTGGGA+AGG 0.594984 1.4:-66990075 MS.gene005221:CDS
GTTGGAGGCAAGAAAGACAT+AGG 0.599190 1.4:+66988622 None:intergenic
TTGAGGAGATTGATGAGTCA+AGG 0.599987 1.4:-66989137 MS.gene005221:CDS
AAGCATGTTAATTACCATTG+GGG 0.601501 1.4:+66988564 None:intergenic
TTGATGAGTCAAGGCTAGAG+AGG 0.603682 1.4:-66989128 MS.gene005221:CDS
TCTCATATTTCTTCATGACA+AGG 0.604054 1.4:-66989086 MS.gene005221:intron
ATATGAGTGCTCATCAGTTG+AGG 0.605741 1.4:-66989154 MS.gene005221:CDS
TAGTCAGGGCAAGTAAATTG+AGG 0.610184 1.4:+66990032 None:intergenic
GGACAAGTCCTTCACCCCAA+TGG 0.617934 1.4:-66988578 MS.gene005221:intron
TGATGAGTCAAGGCTAGAGA+GGG 0.619047 1.4:-66989127 MS.gene005221:CDS
TCACTTCAGCTGGTAAGCAA+GGG 0.625465 1.4:-66988782 MS.gene005221:CDS
GCATGCACTGAAGCACATGA+AGG 0.636267 1.4:-66988915 MS.gene005221:CDS
TGTAAAGGGTGATGAAGACA+AGG 0.640377 1.4:-66989346 MS.gene005221:CDS
GGGTATAAGGGTCAATGGAG+TGG 0.641256 1.4:-66988762 MS.gene005221:CDS
GGGTCAATGGAGTGGCACCT+GGG 0.643479 1.4:-66988754 MS.gene005221:CDS
TTAGACTATACATCTACCAA+AGG 0.664324 1.4:-66988833 MS.gene005221:CDS
AAGGTAGCTGTAATAACAGG+AGG 0.669413 1.4:-66989438 MS.gene005221:CDS
ACATCAGTGAATGCATACAA+AGG 0.684252 1.4:-66988869 MS.gene005221:CDS
GAGTCTTCAACCATTCACCA+CGG 0.723636 1.4:+66988464 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATCTATTATTTACAATATT+TGG - chr1.4:66988894-66988913 MS.gene005221:CDS 10.0%
!! TATAAGAAATAATTTGATAC+AGG + chr1.4:66989027-66989046 None:intergenic 15.0%
!! TGATGTTTACATTAATTAAT+TGG - chr1.4:66990052-66990071 MS.gene005221:CDS 15.0%
!! TTCAATATAACTATATGAAT+TGG + chr1.4:66988640-66988659 None:intergenic 15.0%
!! TTGATTTAATATATATGACA+AGG - chr1.4:66988811-66988830 MS.gene005221:CDS 15.0%
!!! AATATTTCTTTTTCAATTTG+AGG - chr1.4:66988740-66988759 MS.gene005221:CDS 15.0%
!!! GTTATTTTTCTAAGTTATTA+TGG + chr1.4:66989005-66989024 None:intergenic 15.0%
!!! TTAAGATTAATATGTTTTTC+AGG - chr1.4:66990088-66990107 MS.gene005221:CDS 15.0%
!!! TTTTTTAATTTGGAACAAAA+TGG + chr1.4:66988666-66988685 None:intergenic 15.0%
!! ATATAACTATATGAATTGGT+TGG + chr1.4:66988636-66988655 None:intergenic 20.0%
!! CTCAAACTTACTATTAAAAT+AGG + chr1.4:66988845-66988864 None:intergenic 20.0%
!! TGTTAACAAGAATATCAATA+TGG + chr1.4:66989393-66989412 None:intergenic 20.0%
! GTTGCATTTACATATGTAAA+GGG - chr1.4:66989220-66989239 MS.gene005221:CDS 25.0%
! TAAGAAACTTATGATATGTC+CGG - chr1.4:66988957-66988976 MS.gene005221:intron 25.0%
! TAAGCATGTTAATTACCATT+GGG + chr1.4:66990020-66990039 None:intergenic 25.0%
! TTAAAAGATGCTGGTATTAA+TGG + chr1.4:66989864-66989883 None:intergenic 25.0%
! TTAAGCATGTTAATTACCAT+TGG + chr1.4:66990021-66990040 None:intergenic 25.0%
!! ATTAATACCAGCATCTTTTA+AGG - chr1.4:66989863-66989882 MS.gene005221:intron 25.0%
!! TATACTTGAAGTTTGTTTGA+TGG + chr1.4:66988578-66988597 None:intergenic 25.0%
!! TTTTCTTGTGTTAAACTTCA+GGG - chr1.4:66989118-66989137 MS.gene005221:CDS 25.0%
!! TTTTTCTTGTGTTAAACTTC+AGG - chr1.4:66989117-66989136 MS.gene005221:CDS 25.0%
!!! AGATTAATATGTTTTTCAGG+TGG - chr1.4:66990091-66990110 MS.gene005221:CDS 25.0%
!!! TGCAATTTATTTTCCTTAGA+AGG - chr1.4:66989190-66989209 MS.gene005221:CDS 25.0%
!!! TTTTTTATTAGAAGTTGTCC+CGG + chr1.4:66988979-66988998 None:intergenic 25.0%
AAAATAAGAGGAACATTGGT+TGG + chr1.4:66989979-66989998 None:intergenic 30.0%
AAGAAACTTATGATATGTCC+GGG - chr1.4:66988958-66988977 MS.gene005221:intron 30.0%
AAGCATGTTAATTACCATTG+GGG + chr1.4:66990019-66990038 None:intergenic 30.0%
ATAAATCAACATCAACCCAA+TGG + chr1.4:66988873-66988892 None:intergenic 30.0%
CAGTAAAATAAGAGGAACAT+TGG + chr1.4:66989983-66990002 None:intergenic 30.0%
GAAAAAAAGGTGACAAATGA+AGG + chr1.4:66988609-66988628 None:intergenic 30.0%
GATGAGATTGTTAATGCTTA+TGG - chr1.4:66989367-66989386 MS.gene005221:CDS 30.0%
GGTTGCATTTACATATGTAA+AGG - chr1.4:66989219-66989238 MS.gene005221:CDS 30.0%
TAAGTTTGAGTATCACCATT+GGG - chr1.4:66988855-66988874 MS.gene005221:CDS 30.0%
TAATTGTACCATATATGTGC+AGG - chr1.4:66989643-66989662 MS.gene005221:intron 30.0%
TCTCATATTTCTTCATGACA+AGG - chr1.4:66989494-66989513 MS.gene005221:intron 30.0%
TTAGACTATACATCTACCAA+AGG - chr1.4:66989747-66989766 MS.gene005221:intron 30.0%
! ACTATATGAATTGGTTGGTA+TGG + chr1.4:66988631-66988650 None:intergenic 30.0%
! ATTGGTTGGTATGGAAAAAA+AGG + chr1.4:66988622-66988641 None:intergenic 30.0%
!!! CAATGTTCCTCTTATTTTAC+TGG - chr1.4:66989981-66990000 MS.gene005221:intron 30.0%
ACATCAGTGAATGCATACAA+AGG - chr1.4:66989711-66989730 MS.gene005221:intron 35.0%
AGGACACACTAGAAATGATA+AGG - chr1.4:66989263-66989282 MS.gene005221:CDS 35.0%
GACTTGTCCAGTAAAATAAG+AGG + chr1.4:66989991-66990010 None:intergenic 35.0%
GGTATTAATGGTGTCCATAT+GGG + chr1.4:66989852-66989871 None:intergenic 35.0%
GTAAGTTTGAGTATCACCAT+TGG - chr1.4:66988854-66988873 MS.gene005221:CDS 35.0%
TCGAATATAACCACATGTGT+TGG + chr1.4:66989569-66989588 None:intergenic 35.0%
TGGTATTAATGGTGTCCATA+TGG + chr1.4:66989853-66989872 None:intergenic 35.0%
TTGTGTTAAACTTCAGGGTA+AGG - chr1.4:66989123-66989142 MS.gene005221:CDS 35.0%
! TGCAATTGTGGCTTTTACAA+GGG - chr1.4:66989770-66989789 MS.gene005221:intron 35.0%
!! AATACCAGCATCTTTTAAGG+AGG - chr1.4:66989866-66989885 MS.gene005221:intron 35.0%
!!! ATTTTCCTTAGAAGGTGCTA+CGG - chr1.4:66989198-66989217 MS.gene005221:CDS 35.0%
AAAAGCCACAATTGCACCTT+TGG + chr1.4:66989766-66989785 None:intergenic 40.0%
AAGCAACGTCGTAGATACTA+TGG + chr1.4:66989597-66989616 None:intergenic 40.0%
AAGGTAGCTGTAATAACAGG+AGG - chr1.4:66989142-66989161 MS.gene005221:CDS 40.0%
ATATGAGTGCTCATCAGTTG+AGG - chr1.4:66989426-66989445 MS.gene005221:CDS 40.0%
CTACGACGTTGCTTATTCAT+CGG - chr1.4:66989603-66989622 MS.gene005221:intron 40.0%
GAAGAAACTGCTCAATTTGG+TGG - chr1.4:66989888-66989907 MS.gene005221:intron 40.0%
GAGGAAGAAACTGCTCAATT+TGG - chr1.4:66989885-66989904 MS.gene005221:intron 40.0%
GGTAAGGTAGCTGTAATAAC+AGG - chr1.4:66989139-66989158 MS.gene005221:CDS 40.0%
GTTAATTACCATTGGGGTGA+AGG + chr1.4:66990013-66990032 None:intergenic 40.0%
TAGTCAGGGCAAGTAAATTG+AGG + chr1.4:66988551-66988570 None:intergenic 40.0%
TCTTCCTCCTTAAAAGATGC+TGG + chr1.4:66989873-66989892 None:intergenic 40.0%
TGATGAAGACAAGGATGCTA+AGG - chr1.4:66989243-66989262 MS.gene005221:CDS 40.0%
TGATGAGAATTGCAAGAGAG+TGG - chr1.4:66989342-66989361 MS.gene005221:CDS 40.0%
TGTAAAGGGTGATGAAGACA+AGG - chr1.4:66989234-66989253 MS.gene005221:CDS 40.0%
TTGAGGAGATTGATGAGTCA+AGG - chr1.4:66989443-66989462 MS.gene005221:CDS 40.0%
! ATAAGAGGAACATTGGTTGG+AGG + chr1.4:66989976-66989995 None:intergenic 40.0%
! ATCTACCAAAGGTGCAATTG+TGG - chr1.4:66989758-66989777 MS.gene005221:intron 40.0%
! GCAATTGTGGCTTTTACAAG+GGG - chr1.4:66989771-66989790 MS.gene005221:intron 40.0%
! GTGCAATTGTGGCTTTTACA+AGG - chr1.4:66989769-66989788 MS.gene005221:intron 40.0%
! TGTTTGATGGCTTGTAGTCA+GGG + chr1.4:66988565-66988584 None:intergenic 40.0%
! TTGTTTGATGGCTTGTAGTC+AGG + chr1.4:66988566-66988585 None:intergenic 40.0%
AAAAGCAAGAGACACAGCCT+GGG - chr1.4:66988501-66988520 MS.gene005221:intron 45.0%
AAATCAGCTGCTACAGCCAT+TGG + chr1.4:66989316-66989335 None:intergenic 45.0%
AATGGCTGTAGCAGCTGATT+TGG - chr1.4:66989315-66989334 MS.gene005221:CDS 45.0%
AGTGCATGCCTGCACATATA+TGG + chr1.4:66989654-66989673 None:intergenic 45.0%
ATCACTTCAGCTGGTAAGCA+AGG - chr1.4:66989797-66989816 MS.gene005221:intron 45.0%
CAAGTGCCTATGAAAAGAGC+TGG - chr1.4:66989912-66989931 MS.gene005221:intron 45.0%
CACTGAAGCACATGAAGGAA+GGG - chr1.4:66989670-66989689 MS.gene005221:intron 45.0%
GAGGCAAGAAAGACATAGGA+TGG + chr1.4:66989957-66989976 None:intergenic 45.0%
GTAATAACAGGAGGTGACTC+TGG - chr1.4:66989151-66989170 MS.gene005221:CDS 45.0%
GTTGGAGGCAAGAAAGACAT+AGG + chr1.4:66989961-66989980 None:intergenic 45.0%
TAGGATGGAGCAACCTCAAT+AGG + chr1.4:66989942-66989961 None:intergenic 45.0%
TCACTTCAGCTGGTAAGCAA+GGG - chr1.4:66989798-66989817 MS.gene005221:intron 45.0%
TCCATAACATGTTCCTTCCC+AGG + chr1.4:66988521-66988540 None:intergenic 45.0%
TGATGAGTCAAGGCTAGAGA+GGG - chr1.4:66989453-66989472 MS.gene005221:intron 45.0%
TGCTGATGCTAAGGATCCAA+TGG - chr1.4:66989297-66989316 MS.gene005221:CDS 45.0%
TGTTACGTGTCCAACACATG+TGG - chr1.4:66989556-66989575 MS.gene005221:intron 45.0%
TTGATGAGTCAAGGCTAGAG+AGG - chr1.4:66989452-66989471 MS.gene005221:intron 45.0%
! AGCAAGGGTATAAGGGTCAA+TGG - chr1.4:66989813-66989832 MS.gene005221:intron 45.0%
! AGCTGGTAAGCAAGGGTATA+AGG - chr1.4:66989805-66989824 MS.gene005221:intron 45.0%
! ATGGCTGTAGCAGCTGATTT+GGG - chr1.4:66989316-66989335 MS.gene005221:CDS 45.0%
! GCTGGTAAGCAAGGGTATAA+GGG - chr1.4:66989806-66989825 MS.gene005221:intron 45.0%
!! CTGTGTCTCTTGCTTTTGTG+GGG + chr1.4:66988498-66988517 None:intergenic 45.0%
!! GCTGTGTCTCTTGCTTTTGT+GGG + chr1.4:66988499-66988518 None:intergenic 45.0%
!! TGCAAAGAGTGCTGATGCTA+AGG - chr1.4:66989288-66989307 MS.gene005221:CDS 45.0%
!! TGTGTCTCTTGCTTTTGTGG+GGG + chr1.4:66988497-66988516 None:intergenic 45.0%
!!! ATGTTTTTCAGGTGGAACCG+TGG - chr1.4:66990099-66990118 MS.gene005221:CDS 45.0%
!!! ATTGTATAATTTTTTTAATT+TGG + chr1.4:66988676-66988695 None:intergenic 5.0%
AAGAGCTGGTCAGCCTATTG+AGG - chr1.4:66989926-66989945 MS.gene005221:intron 50.0%
AAGGGGACTATCACTTCAGC+TGG - chr1.4:66989788-66989807 MS.gene005221:intron 50.0%
CAAAAGCAAGAGACACAGCC+TGG - chr1.4:66988500-66988519 MS.gene005221:intron 50.0%
GCACTGAAGCACATGAAGGA+AGG - chr1.4:66989669-66989688 MS.gene005221:intron 50.0%
GCATGCACTGAAGCACATGA+AGG - chr1.4:66989665-66989684 MS.gene005221:intron 50.0%
GCCTGGGAAGGAACATGTTA+TGG - chr1.4:66988517-66988536 MS.gene005221:intron 50.0%
GGGCAAGTAAATTGAGGCAG+CGG + chr1.4:66988545-66988564 None:intergenic 50.0%
GGGTATAAGGGTCAATGGAG+TGG - chr1.4:66989818-66989837 MS.gene005221:intron 50.0%
TGCAACCGTAGCACCTTCTA+AGG + chr1.4:66989206-66989225 None:intergenic 50.0%
! GGCTGACCAGCTCTTTTCAT+AGG + chr1.4:66989921-66989940 None:intergenic 50.0%
!! ACAGGAGGTGACTCTGGAAT+TGG - chr1.4:66989157-66989176 MS.gene005221:CDS 50.0%
!! CAGAAGTGTCAGTGCTACAG+AGG + chr1.4:66989526-66989545 None:intergenic 50.0%
!! GGCTGTGTCTCTTGCTTTTG+TGG + chr1.4:66988500-66988519 None:intergenic 50.0%
!! GTGTCTCTTGCTTTTGTGGG+GGG + chr1.4:66988496-66988515 None:intergenic 50.0%
AATGGTGTCCATATGGGCCC+AGG + chr1.4:66989846-66989865 None:intergenic 55.0%
GGACAAGTCCTTCACCCCAA+TGG - chr1.4:66990002-66990021 MS.gene005221:intron 55.0%
TCAGGTGGAACCGTGGTGAA+TGG - chr1.4:66990106-66990125 MS.gene005221:CDS 55.0%
AGGGTCAATGGAGTGGCACC+TGG - chr1.4:66989825-66989844 MS.gene005221:intron 60.0%
GCAAGAGACACAGCCTGGGA+AGG - chr1.4:66988505-66988524 MS.gene005221:intron 60.0%
GGGTCAATGGAGTGGCACCT+GGG - chr1.4:66989826-66989845 MS.gene005221:intron 60.0%
GAGTGGCACCTGGGCCCATA+TGG - chr1.4:66989835-66989854 MS.gene005221:intron 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 66988470 66990132 66988470 ID=MS.gene005221
chr1.4 mRNA 66988470 66990132 66988470 ID=MS.gene005221.t1;Parent=MS.gene005221
chr1.4 exon 66990010 66990132 66990010 ID=MS.gene005221.t1.exon1;Parent=MS.gene005221.t1
chr1.4 CDS 66990010 66990132 66990010 ID=cds.MS.gene005221.t1;Parent=MS.gene005221.t1
chr1.4 exon 66989087 66989463 66989087 ID=MS.gene005221.t1.exon2;Parent=MS.gene005221.t1
chr1.4 CDS 66989087 66989463 66989087 ID=cds.MS.gene005221.t1;Parent=MS.gene005221.t1
chr1.4 exon 66988579 66988937 66988579 ID=MS.gene005221.t1.exon3;Parent=MS.gene005221.t1
chr1.4 CDS 66988579 66988937 66988579 ID=cds.MS.gene005221.t1;Parent=MS.gene005221.t1
chr1.4 exon 66988470 66988492 66988470 ID=MS.gene005221.t1.exon4;Parent=MS.gene005221.t1
chr1.4 CDS 66988470 66988492 66988470 ID=cds.MS.gene005221.t1;Parent=MS.gene005221.t1
Gene Sequence

>MS.gene005221

ATGGCTTCAGGTGAACAGAAATTCCCCCCACAAAAGCAAGAGACACAGCCTGGGAAGGAACATGTTATGGATCCGCTGCCTCAATTTACTTGCCCTGACTACAAGCCATCAAACAAACTTCAAGTATATATATTCACCTTCATTTGTCACCTTTTTTTCCATACCAACCAATTCATATAGTTATATTGAATCACCATTTTGTTCCAAATTAAAAAAATTATACAATTGAGTCATCTTGTTTACAAAATGTGAAAATCATTCTTAATTTAAAATATTTCTTTTTCAATTTGAGGATTTTATATTTGATGCTTATAATTTTGAGTATCAAAACAGTTATTTGTTTGATTTAATATATATGACAAGGTTCATAGACCTATTTTAATAGTAAGTTTGAGTATCACCATTGGGTTGATGTTGATTTATTTATCTATTATTTACAATATTTGGATTGATTTATTTGAGTTAAAATTATTTGAGAGTGTTTATATAAGAAACTTATGATATGTCCGGGACAACTTCTAATAAAAAAAATCCATAATAACTTAGAAAAATAACCTGTATCAAATTATTTCTTATATTTTTTACAAAAATAGTTTATGCATAAATACATGTACACAAACGATTAATTAATTTGTTTATACATGTTATTTTTCTTGTGTTAAACTTCAGGGTAAGGTAGCTGTAATAACAGGAGGTGACTCTGGAATTGGACGAGCAGTATGCAATTTATTTTCCTTAGAAGGTGCTACGGTTGCATTTACATATGTAAAGGGTGATGAAGACAAGGATGCTAAGGACACACTAGAAATGATAAGGAATGCAAAGAGTGCTGATGCTAAGGATCCAATGGCTGTAGCAGCTGATTTGGGTTTTGATGAGAATTGCAAGAGAGTGGTTGATGAGATTGTTAATGCTTATGGCCATATTGATATTCTTGTTAACAATGCTGCTGAGCAATATGAGTGCTCATCAGTTGAGGAGATTGATGAGTCAAGGCTAGAGAGGGTGTTCAGAACAAATATCTTCTCATATTTCTTCATGACAAGGTCAGTACCTCTGTAGCACTGACACTTCTGATAAAAGTTATGTTACGTGTCCAACACATGTGGTTATATTCGATTAATCCATAGTATCTACGACGTTGCTTATTCATCGGTAAACATCAAGTATATATAATTGTACCATATATGTGCAGGCATGCACTGAAGCACATGAAGGAAGGGAGCAGCATTATAAACACAACATCAGTGAATGCATACAAAGGAAATGCAAAGCTATTAGACTATACATCTACCAAAGGTGCAATTGTGGCTTTTACAAGGGGACTATCACTTCAGCTGGTAAGCAAGGGTATAAGGGTCAATGGAGTGGCACCTGGGCCCATATGGACACCATTAATACCAGCATCTTTTAAGGAGGAAGAAACTGCTCAATTTGGTGGTCAAGTGCCTATGAAAAGAGCTGGTCAGCCTATTGAGGTTGCTCCATCCTATGTCTTTCTTGCCTCCAACCAATGTTCCTCTTATTTTACTGGACAAGTCCTTCACCCCAATGGTAATTAACATGCTTAAATTCTTGCTTCTGATGTTTACATTAATTAATTGGAGAAGTTTACTGATTAAGATTAATATGTTTTTCAGGTGGAACCGTGGTGAATGGTTGA
Protein sequence

>MS.gene005221.t1

MASGEQKFPPQKQETQPGKEHVMDPLPQFTCPDYKPSNKLQGKVAVITGGDSGIGRAVCNLFSLEGATVAFTYVKGDEDKDAKDTLEMIRNAKSADAKDPMAVAADLGFDENCKRVVDEIVNAYGHIDILVNNAAEQYECSSVEEIDESRLERVFRTNIFSYFFMTRHALKHMKEGSSIINTTSVNAYKGNAKLLDYTSTKGAIVAFTRGLSLQLVSKGIRVNGVAPGPIWTPLIPASFKEEETAQFGGQVPMKRAGQPIEVAPSYVFLASNQCSSYFTGQVLHPNGGTVVNG