AlfalfaGEDB | MS.gene31507

M. sativa cultivar XinJiangDaYe / MS.gene31507

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016491
PFAM: PF13561.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31507.t1	XP_013465058.1	95.8	264	11	0	1	264	1	264	9.90E-137	496.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31507.t1	Q94K41	60.8	255	100	0	1	255	1	255	6.4e-84	312.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31507.t1	A0A072VCB0	95.8	264	11	0	1	264	1	264	7.1e-137	496.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050780	MS.gene31507	0.820908	5.35E-53	-1.69E-46
MS.gene05468	MS.gene31507	0.806865	6.68E-50	-1.69E-46
MS.gene055846	MS.gene31507	0.855512	6.00E-62	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene63300	MS.gene31507	PPI
MS.gene008162	MS.gene31507	PPI
MS.gene006999	MS.gene31507	PPI
MS.gene021164	MS.gene31507	PPI
MS.gene008160	MS.gene31507	PPI
MS.gene95020	MS.gene31507	PPI
MS.gene31507	MS.gene003729	PPI
MS.gene050862	MS.gene31507	PPI
MS.gene065380	MS.gene31507	PPI
MS.gene31507	MS.gene43481	PPI
MS.gene50524	MS.gene31507	PPI
MS.gene42856	MS.gene31507	PPI
MS.gene31507	MS.gene44088	PPI
MS.gene63403	MS.gene31507	PPI
MS.gene019786	MS.gene31507	PPI
MS.gene52743	MS.gene31507	PPI
MS.gene043387	MS.gene31507	PPI
MS.gene31507	MS.gene07097	PPI
MS.gene25667	MS.gene31507	PPI
MS.gene066728	MS.gene31507	PPI
MS.gene08756	MS.gene31507	PPI
MS.gene85724	MS.gene31507	PPI
MS.gene08695	MS.gene31507	PPI
MS.gene63306	MS.gene31507	PPI
MS.gene62187	MS.gene31507	PPI
MS.gene005221	MS.gene31507	PPI
MS.gene80360	MS.gene31507	PPI
MS.gene024269	MS.gene31507	PPI
MS.gene033685	MS.gene31507	PPI
MS.gene42114	MS.gene31507	PPI
MS.gene056029	MS.gene31507	PPI
MS.gene73824	MS.gene31507	PPI
MS.gene014425	MS.gene31507	PPI
MS.gene63482	MS.gene31507	PPI
MS.gene31507	MS.gene50524	PPI
MS.gene63483	MS.gene31507	PPI
MS.gene04700	MS.gene31507	PPI
MS.gene045581	MS.gene31507	PPI
MS.gene007002	MS.gene31507	PPI
MS.gene055846	MS.gene31507	PPI
MS.gene050431	MS.gene31507	PPI
MS.gene050131	MS.gene31507	PPI
MS.gene003925	MS.gene31507	PPI
MS.gene62193	MS.gene31507	PPI
MS.gene31507	MS.gene04700	PPI
MS.gene41122	MS.gene31507	PPI
MS.gene29327	MS.gene31507	PPI
MS.gene31507	MS.gene08695	PPI
MS.gene073684	MS.gene31507	PPI
MS.gene63308	MS.gene31507	PPI
MS.gene07096	MS.gene31507	PPI
MS.gene54952	MS.gene31507	PPI
MS.gene62195	MS.gene31507	PPI
MS.gene008153	MS.gene31507	PPI
MS.gene042142	MS.gene31507	PPI
MS.gene23019	MS.gene31507	PPI
MS.gene25674	MS.gene31507	PPI
MS.gene31507	MS.gene039828	PPI
MS.gene012794	MS.gene31507	PPI
MS.gene018066	MS.gene31507	PPI
MS.gene072664	MS.gene31507	PPI
MS.gene28848	MS.gene31507	PPI
MS.gene87026	MS.gene31507	PPI
MS.gene31507	MS.gene30551	PPI
MS.gene68956	MS.gene31507	PPI
MS.gene62194	MS.gene31507	PPI
MS.gene63307	MS.gene31507	PPI
MS.gene87780	MS.gene31507	PPI
MS.gene006617	MS.gene31507	PPI
MS.gene54598	MS.gene31507	PPI
MS.gene028938	MS.gene31507	PPI
MS.gene73088	MS.gene31507	PPI
MS.gene056339	MS.gene31507	PPI
MS.gene03319	MS.gene31507	PPI
MS.gene031693	MS.gene31507	PPI
MS.gene050432	MS.gene31507	PPI
MS.gene68880	MS.gene31507	PPI
MS.gene42260	MS.gene31507	PPI
MS.gene08694	MS.gene31507	PPI
MS.gene064756	MS.gene31507	PPI
MS.gene003926	MS.gene31507	PPI
MS.gene52742	MS.gene31507	PPI
MS.gene007006	MS.gene31507	PPI
MS.gene008161	MS.gene31507	PPI
MS.gene31507	MS.gene072462	PPI
MS.gene31507	MS.gene003992	PPI
MS.gene50522	MS.gene31507	PPI
MS.gene28847	MS.gene31507	PPI
MS.gene31507	MS.gene055846	PPI
MS.gene007678	MS.gene31507	PPI
MS.gene07097	MS.gene31507	PPI
MS.gene044694	MS.gene31507	PPI
MS.gene63484	MS.gene31507	PPI
MS.gene055565	MS.gene31507	PPI
MS.gene012681	MS.gene31507	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31507.t1	MTR_2g090165	95.833	264	11	0	1	264	1	264	0.0	516
MS.gene31507.t1	MTR_7g110130	81.955	266	46	2	1	264	1	266	5.33e-155	432
MS.gene31507.t1	MTR_7g110160	77.692	260	40	2	1	260	1	242	8.93e-141	395
MS.gene31507.t1	MTR_7g110140	69.349	261	79	1	1	261	1	260	1.25e-129	368
MS.gene31507.t1	MTR_6g035395	63.137	255	81	1	3	257	2	243	2.83e-109	316
MS.gene31507.t1	MTR_6g023590	50.996	251	121	2	5	253	12	262	3.48e-86	258
MS.gene31507.t1	MTR_7g071680	49.821	279	116	5	2	259	25	300	8.24e-81	245
MS.gene31507.t1	MTR_6g023910	50.198	253	124	2	2	252	9	261	1.26e-80	244
MS.gene31507.t1	MTR_6g023950	50.198	253	124	2	5	255	12	264	1.91e-80	243
MS.gene31507.t1	MTR_6g023610	51.200	250	120	2	5	252	12	261	2.25e-80	243
MS.gene31507.t1	MTR_6g023600	49.402	251	125	2	5	253	12	262	8.93e-79	239
MS.gene31507.t1	MTR_4g052340	50.787	254	120	4	4	256	12	261	1.62e-78	238
MS.gene31507.t1	MTR_4g052320	49.609	256	125	3	2	256	4	256	2.54e-78	238
MS.gene31507.t1	MTR_7g105460	48.221	253	129	2	5	256	12	263	4.15e-78	238
MS.gene31507.t1	MTR_6g023990	46.850	254	133	2	2	253	10	263	4.77e-78	237
MS.gene31507.t1	MTR_4g052350	50.196	255	122	3	4	257	12	262	5.59e-78	237
MS.gene31507.t1	MTR_4g052300	49.805	257	122	4	2	256	4	255	6.03e-77	234
MS.gene31507.t1	MTR_6g023600	48.606	251	121	3	5	253	12	256	1.49e-74	228
MS.gene31507.t1	MTR_7g107670	45.703	256	131	4	4	253	31	284	4.88e-71	220
MS.gene31507.t1	MTR_7g083870	46.241	266	133	6	4	261	33	296	2.10e-70	219
MS.gene31507.t1	MTR_4g078630	42.857	273	143	5	1	264	14	282	4.29e-64	202
MS.gene31507.t1	MTR_7g105580	45.211	261	124	4	4	263	6	248	1.14e-63	200
MS.gene31507.t1	MTR_3g020670	43.866	269	140	5	4	263	14	280	3.17e-62	197
MS.gene31507.t1	MTR_7g105590	48.677	189	95	2	4	191	6	193	7.54e-54	173
MS.gene31507.t1	MTR_7g105570	48.066	181	92	2	77	256	41	220	3.91e-49	162
MS.gene31507.t1	MTR_3g113870	44.737	190	102	3	14	202	29	216	4.07e-49	162
MS.gene31507.t1	MTR_4g084800	49.640	139	69	1	4	141	11	149	6.23e-40	135
MS.gene31507.t1	MTR_4g058840	30.545	275	153	6	1	251	1	261	1.95e-25	101
MS.gene31507.t1	MTR_4g058820	30.545	275	153	6	1	251	1	261	1.95e-25	101
MS.gene31507.t1	MTR_6g023940	34.826	201	67	4	2	200	9	147	4.51e-25	97.8
MS.gene31507.t1	MTR_4g058810	29.496	278	158	6	1	254	1	264	3.21e-22	93.2
MS.gene31507.t1	MTR_3g085740	27.519	258	166	9	4	252	73	318	2.90e-21	91.3
MS.gene31507.t1	MTR_4g131740	27.863	262	163	8	5	251	39	289	1.75e-20	89.0
MS.gene31507.t1	MTR_7g113650	26.692	266	169	7	5	256	39	292	2.92e-20	88.2
MS.gene31507.t1	MTR_1g051165	27.757	263	176	5	4	254	12	272	6.89e-20	87.0
MS.gene31507.t1	MTR_1g082760	29.537	281	142	12	5	256	39	292	1.06e-19	86.7
MS.gene31507.t1	MTR_4g132850	28.571	252	169	5	4	252	5	248	1.15e-19	85.9
MS.gene31507.t1	MTR_1196s0010	32.016	253	151	9	7	252	275	513	7.36e-19	85.9
MS.gene31507.t1	MTR_4g064887	26.966	267	173	8	2	259	11	264	1.21e-17	80.5
MS.gene31507.t1	MTR_3g068070	26.744	258	177	7	2	254	11	261	2.16e-15	74.3
MS.gene31507.t1	MTR_7g105560	62.500	56	21	0	4	59	11	66	3.91e-15	71.2
MS.gene31507.t1	MTR_3g068085	27.715	267	172	8	6	263	15	269	6.20e-15	73.2
MS.gene31507.t1	MTR_3g068065	26.877	253	173	7	6	253	17	262	6.24e-15	73.2
MS.gene31507.t1	MTR_8g442750	28.731	268	171	10	6	260	14	274	1.02e-14	72.8
MS.gene31507.t1	MTR_4g064887	29.000	200	131	5	2	195	11	205	1.95e-14	70.9
MS.gene31507.t1	MTR_3g068175	29.213	267	178	9	1	263	12	271	2.28e-14	71.6
MS.gene31507.t1	MTR_7g063330	29.119	261	168	9	2	253	11	263	5.30e-14	70.5
MS.gene31507.t1	MTR_4g058830	31.098	164	99	3	95	251	51	207	1.12e-13	68.6
MS.gene31507.t1	MTR_4g086410	27.203	261	149	7	8	248	6	245	2.20e-13	70.1
MS.gene31507.t1	MTR_3g068155	28.016	257	175	9	3	255	16	266	2.26e-13	68.6
MS.gene31507.t1	MTR_7g094520	29.688	256	164	8	6	252	11	259	4.38e-13	67.8
MS.gene31507.t1	MTR_8g096580	28.194	227	143	6	4	225	43	254	9.73e-13	67.4
MS.gene31507.t1	MTR_3g087860	33.094	139	87	3	56	192	59	193	1.22e-12	66.6
MS.gene31507.t1	MTR_4g086400	27.536	207	128	4	8	197	6	207	1.30e-12	67.8
MS.gene31507.t1	MTR_3g068175	28.782	271	178	10	1	263	12	275	2.62e-12	65.9
MS.gene31507.t1	MTR_7g063320	29.389	262	165	9	2	252	11	263	7.72e-12	64.3
MS.gene31507.t1	MTR_7g063360	27.757	263	172	9	2	254	12	266	1.09e-11	63.9
MS.gene31507.t1	MTR_4g064883	26.459	257	177	8	3	254	11	260	3.50e-11	62.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31507.t1	AT2G47140	60.784	255	100	0	1	255	1	255	1.92e-111	322
MS.gene31507.t1	AT3G29250	58.846	260	99	2	1	256	1	256	1.25e-102	299
MS.gene31507.t1	AT3G29250	58.846	260	99	2	1	256	37	292	3.11e-102	300
MS.gene31507.t1	AT2G47130	58.301	259	101	2	1	256	1	255	3.23e-102	298
MS.gene31507.t1	AT3G29250	58.527	258	99	2	3	256	41	294	3.29e-101	297
MS.gene31507.t1	AT2G47120	56.923	260	102	4	1	256	1	254	7.27e-101	295
MS.gene31507.t1	AT3G29260	57.915	259	102	2	1	256	1	255	5.61e-100	293
MS.gene31507.t1	AT2G47120	57.031	256	100	4	5	256	6	255	5.99e-100	292
MS.gene31507.t1	AT3G51680	48.162	272	125	3	4	259	30	301	2.21e-76	234
MS.gene31507.t1	AT3G26770	42.910	268	141	5	4	261	39	304	2.76e-64	203
MS.gene31507.t1	AT4G03140	43.411	258	138	4	4	255	76	331	1.18e-62	200
MS.gene31507.t1	AT1G52340	46.067	267	126	6	4	256	16	278	1.73e-62	198
MS.gene31507.t1	AT3G26760	39.630	270	151	5	2	261	32	299	9.46e-61	194
MS.gene31507.t1	AT3G42960	44.444	261	133	3	2	253	4	261	4.33e-55	179
MS.gene31507.t1	AT2G47150	37.450	251	104	7	6	256	1	198	4.24e-38	132
MS.gene31507.t1	AT3G55290	29.070	258	164	5	6	251	17	267	1.44e-21	91.7
MS.gene31507.t1	AT3G55290	29.070	258	164	5	6	251	18	268	1.50e-21	91.7
MS.gene31507.t1	AT1G07450	29.119	261	168	7	1	254	3	253	1.31e-20	88.6
MS.gene31507.t1	AT1G62610	28.846	260	163	6	6	251	15	266	3.26e-20	87.8
MS.gene31507.t1	AT1G62610	28.846	260	163	6	6	251	19	270	3.53e-20	87.8
MS.gene31507.t1	AT1G62610	28.846	260	163	6	6	251	13	264	3.64e-20	87.8
MS.gene31507.t1	AT1G62610	28.846	260	163	6	6	251	14	265	3.81e-20	87.8
MS.gene31507.t1	AT3G55310	29.323	266	153	7	6	251	17	267	3.01e-19	85.1
MS.gene31507.t1	AT4G05530	31.102	254	160	7	4	252	7	250	4.51e-19	84.3
MS.gene31507.t1	AT2G29310	28.148	270	156	8	3	254	4	253	5.50e-19	84.3
MS.gene31507.t1	AT2G29340	29.167	264	161	8	3	254	4	253	6.28e-19	84.0
MS.gene31507.t1	AT2G29340	29.167	264	161	8	3	254	4	253	1.02e-18	84.3
MS.gene31507.t1	AT3G46170	27.132	258	169	5	6	251	26	276	1.41e-18	83.6
MS.gene31507.t1	AT1G63380	26.718	262	172	5	6	254	15	269	1.56e-18	83.2
MS.gene31507.t1	AT1G63380	26.718	262	172	5	6	254	22	276	1.81e-18	83.2
MS.gene31507.t1	AT1G63380	26.718	262	172	5	6	254	17	271	1.93e-18	83.2
MS.gene31507.t1	AT1G63380	26.718	262	172	5	6	254	19	273	2.30e-18	82.8
MS.gene31507.t1	AT1G24360	25.581	258	175	8	4	254	72	319	7.99e-18	82.0
MS.gene31507.t1	AT2G17845	27.925	265	169	6	6	255	47	304	1.88e-17	80.9
MS.gene31507.t1	AT2G29150	28.571	259	170	7	2	254	12	261	2.42e-17	79.7
MS.gene31507.t1	AT1G54870	26.316	266	170	7	5	256	83	336	5.19e-17	79.7
MS.gene31507.t1	AT1G54870	26.316	266	170	7	5	256	81	334	6.15e-17	79.7
MS.gene31507.t1	AT5G06060	29.845	258	167	8	3	254	6	255	1.28e-16	77.8
MS.gene31507.t1	AT2G30670	27.132	258	174	6	3	254	4	253	1.41e-16	77.4
MS.gene31507.t1	AT2G29290	28.030	264	164	6	3	254	4	253	1.58e-16	77.4
MS.gene31507.t1	AT1G07440	27.843	255	175	7	3	254	9	257	3.14e-16	76.6
MS.gene31507.t1	AT2G29350	26.641	259	175	7	2	254	11	260	2.43e-15	74.3
MS.gene31507.t1	AT2G29370	27.444	266	175	8	3	260	13	268	2.45e-15	74.3
MS.gene31507.t1	AT3G05260	27.273	264	166	8	5	254	36	287	4.17e-15	73.9
MS.gene31507.t1	AT2G29360	27.481	262	168	9	3	254	13	262	4.20e-15	73.6
MS.gene31507.t1	AT2G29260	29.771	262	166	10	2	254	62	314	1.51e-14	72.8
MS.gene31507.t1	AT2G29340	30.151	199	128	7	3	195	4	197	2.01e-14	70.5
MS.gene31507.t1	AT2G29340	30.151	199	128	7	3	195	4	197	2.01e-14	70.5
MS.gene31507.t1	AT2G29290	28.500	200	137	4	56	254	42	236	2.18e-14	71.2
MS.gene31507.t1	AT2G29300	27.652	264	176	8	3	259	4	259	2.74e-14	71.6
MS.gene31507.t1	AT2G29300	28.571	259	170	9	3	254	4	254	2.78e-14	71.2
MS.gene31507.t1	AT1G49670	30.045	223	125	7	8	210	6	217	4.07e-14	72.4
MS.gene31507.t1	AT1G49670	30.045	223	125	7	8	210	6	217	4.93e-14	72.0
MS.gene31507.t1	AT4G13180	31.801	261	156	10	6	254	13	263	7.37e-14	70.1
MS.gene31507.t1	AT3G12800	28.044	271	172	11	6	261	10	272	7.78e-14	70.5
MS.gene31507.t1	AT1G10310	33.333	132	86	2	63	194	73	202	1.64e-13	68.6
MS.gene31507.t1	AT2G29320	29.297	256	166	9	6	254	13	260	2.89e-13	68.6
MS.gene31507.t1	AT2G29330	26.953	256	177	6	3	254	4	253	5.80e-13	67.4
MS.gene31507.t1	AT2G29310	28.019	207	122	6	3	195	4	197	6.52e-13	66.2
MS.gene31507.t1	AT5G50770	28.796	191	128	5	1	187	40	226	2.56e-12	66.2
MS.gene31507.t1	AT2G29350	26.500	200	140	5	56	254	29	222	2.98e-12	65.1
MS.gene31507.t1	AT5G18210	28.731	268	162	9	2	252	4	259	1.05e-11	63.9
MS.gene31507.t1	AT2G29310	26.141	241	140	8	32	254	31	251	1.09e-11	63.9
MS.gene31507.t1	AT2G29350	27.500	200	134	5	2	195	11	205	1.19e-11	63.2

Find 53 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCAGTGAGCTTGGAGCTTA+TGG	0.278067	2.4:-10871931	MS.gene31507:CDS
AGCAGATATCAATGATGAAT+TGG	0.283540	2.4:-10872334	MS.gene31507:CDS
TGGTGTTGTCAAATTCATTT+AGG	0.283555	2.4:+10872133	None:intergenic
GCATCAAAACATGGTCTTAT+AGG	0.284802	2.4:-10871970	MS.gene31507:CDS
CCTTGATTATGCTAATTTGC+AGG	0.303341	2.4:-10871815	MS.gene31507:CDS
CATCATGTTGCAAATTCAAT+AGG	0.308261	2.4:-10872309	MS.gene31507:CDS
GTAAAGATATTCGTGGAAAA+TGG	0.310172	2.4:-10872372	MS.gene31507:CDS
TACACTGGCAGTGCAAATTA+TGG	0.353051	2.4:+10872032	None:intergenic
GATGAATCTGCTTATATTAG+TGG	0.356858	2.4:-10871739	MS.gene31507:CDS
ATGGTCTTATAGGACTTGTT+AGG	0.392057	2.4:-10871960	MS.gene31507:CDS
GCTTTCACGTTGGAGAGATA+TGG	0.399630	2.4:-10872222	MS.gene31507:CDS
ACTGGGGCAGCAAGCGGAAT+AGG	0.399759	2.4:-10872405	MS.gene31507:CDS
GCCAACAGCTTCGACTTCAC+TGG	0.415316	2.4:+10871837	None:intergenic
ATTTGCACTGCCAGTGTAGC+TGG	0.419113	2.4:-10872027	MS.gene31507:CDS
CAACTTGTTTCTCATCTCTC+AGG	0.421960	2.4:+10872256	None:intergenic
ATTGCTGAACATGATGTCTA+AGG	0.424678	2.4:+10872197	None:intergenic
TGATCATAGGTTGGAGGGTA+AGG	0.428592	2.4:-10872439	MS.gene31507:intron
AATTCTAGTATGCTAGATAA+AGG	0.435820	2.4:+10872159	None:intergenic
ATCATGTTGCAAATTCAATA+GGG	0.440902	2.4:-10872308	MS.gene31507:CDS
TTATATTAGTGGACATAACT+TGG	0.444616	2.4:-10871728	MS.gene31507:CDS
GACATCATGTTCAGCAATGC+TGG	0.459929	2.4:-10872192	MS.gene31507:CDS
GCAGATATCAATGATGAATT+GGG	0.463215	2.4:-10872333	MS.gene31507:CDS
GTGGCTTCTCAGTCATTAAT+CGG	0.474841	2.4:-10871696	MS.gene31507:CDS
TTCAGCAATGCTGGTAATGC+TGG	0.487409	2.4:-10872183	MS.gene31507:CDS
TTTGCAGGGAATTACATTGA+AGG	0.494365	2.4:-10871800	MS.gene31507:CDS
GGACATAACTTGGTCGTTGA+TGG	0.497040	2.4:-10871718	MS.gene31507:CDS
AGAGGTGTAGCAACAGCATA+AGG	0.501265	2.4:+10871886	None:intergenic
GGTAAGGTAGCTATAGTGAC+TGG	0.506112	2.4:-10872423	MS.gene31507:CDS
CTTGATTATGCTAATTTGCA+GGG	0.512554	2.4:-10871814	MS.gene31507:CDS
AGGTCAACATGCAGTGAGCT+TGG	0.516394	2.4:-10871940	MS.gene31507:CDS
ACCTCTTGCATGTAGAGCAC+TGG	0.521993	2.4:-10871869	MS.gene31507:CDS
CAAGCATGCTGCACGTGTAA+TGG	0.527529	2.4:-10872076	MS.gene31507:CDS
CCTGCAAATTAGCATAATCA+AGG	0.541425	2.4:+10871815	None:intergenic
ACCAACAAAACCAGCTACAC+TGG	0.550665	2.4:+10872017	None:intergenic
CCCAGTGAAGTCGAAGCTGT+TGG	0.551376	2.4:-10871838	MS.gene31507:CDS
ACCATGGCTGTGAACGTTCG+TGG	0.565494	2.4:-10872114	MS.gene31507:CDS
CCAACAGCTTCGACTTCACT+GGG	0.566318	2.4:+10871838	None:intergenic
ACCACGAACGTTCACAGCCA+TGG	0.577592	2.4:+10872113	None:intergenic
AGAAGCAGTAAAGATATTCG+TGG	0.578218	2.4:-10872379	MS.gene31507:CDS
GTAAGGTAGCTATAGTGACT+GGG	0.582532	2.4:-10872422	MS.gene31507:CDS
TCCAGTGCTCTACATGCAAG+AGG	0.608317	2.4:+10871868	None:intergenic
ATAGTGACTGGGGCAGCAAG+CGG	0.610587	2.4:-10872411	MS.gene31507:CDS
CATAACTTGGTCGTTGATGG+TGG	0.616743	2.4:-10871715	MS.gene31507:CDS
CATCTCTCAGGTCACAGTGG+TGG	0.620723	2.4:+10872268	None:intergenic
GGAAATTGTTGCTTTCACGT+TGG	0.626510	2.4:-10872232	MS.gene31507:CDS
TGCATGTAGAGCACTGGACA+TGG	0.628198	2.4:-10871863	MS.gene31507:CDS
AATCATCAATGTCAAAACAA+AGG	0.629421	2.4:-10872568	None:intergenic
GCATGCTGCACGTGTAATGG+TGG	0.633944	2.4:-10872073	MS.gene31507:CDS
GAGAGATGAGAAACAAGTTG+AGG	0.635217	2.4:-10872253	MS.gene31507:CDS
GATTATACAGCATCAAAACA+TGG	0.649473	2.4:-10871979	MS.gene31507:CDS
TCTCATCTCTCAGGTCACAG+TGG	0.665905	2.4:+10872265	None:intergenic
AAATGAATTTGACAACACCA+TGG	0.674818	2.4:-10872130	MS.gene31507:CDS
TAAGGTAGCTATAGTGACTG+GGG	0.695873	2.4:-10872421	MS.gene31507:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATATTTTATTTTATGATCAT+AGG	-	chr2.4:10871780-10871799	MS.gene31507:CDS	10.0%
!!!	TTTATTTTATGATCATAGGT+TGG	-	chr2.4:10871784-10871803	MS.gene31507:CDS	20.0%
!	AATTCTAGTATGCTAGATAA+AGG	+	chr2.4:10872076-10872095	None:intergenic	25.0%
!	ATCATGTTGCAAATTCAATA+GGG	-	chr2.4:10871924-10871943	MS.gene31507:CDS	25.0%
!!	TTATATTAGTGGACATAACT+TGG	-	chr2.4:10872504-10872523	MS.gene31507:intron	25.0%
	AAATGAATTTGACAACACCA+TGG	-	chr2.4:10872102-10872121	MS.gene31507:CDS	30.0%
	AGCAGATATCAATGATGAAT+TGG	-	chr2.4:10871898-10871917	MS.gene31507:CDS	30.0%
	CATCATGTTGCAAATTCAAT+AGG	-	chr2.4:10871923-10871942	MS.gene31507:CDS	30.0%
	GATGAATCTGCTTATATTAG+TGG	-	chr2.4:10872493-10872512	MS.gene31507:intron	30.0%
	GATTATACAGCATCAAAACA+TGG	-	chr2.4:10872253-10872272	MS.gene31507:CDS	30.0%
	GCAGATATCAATGATGAATT+GGG	-	chr2.4:10871899-10871918	MS.gene31507:CDS	30.0%
	GTAAAGATATTCGTGGAAAA+TGG	-	chr2.4:10871860-10871879	MS.gene31507:CDS	30.0%
	TGTTATAAGCCAATAGAAAC+AGG	+	chr2.4:10871746-10871765	None:intergenic	30.0%
!	CTTGATTATGCTAATTTGCA+GGG	-	chr2.4:10872418-10872437	MS.gene31507:CDS	30.0%
!!	ATTTTATGATCATAGGTTGG+AGG	-	chr2.4:10871787-10871806	MS.gene31507:CDS	30.0%
!!	TGGTGTTGTCAAATTCATTT+AGG	+	chr2.4:10872102-10872121	None:intergenic	30.0%
!!	TTTTATGATCATAGGTTGGA+GGG	-	chr2.4:10871788-10871807	MS.gene31507:CDS	30.0%
	AGAAGCAGTAAAGATATTCG+TGG	-	chr2.4:10871853-10871872	MS.gene31507:CDS	35.0%
	ATTGCTGAACATGATGTCTA+AGG	+	chr2.4:10872038-10872057	None:intergenic	35.0%
	CCTGCAAATTAGCATAATCA+AGG	+	chr2.4:10872420-10872439	None:intergenic	35.0%
	TTTGCAGGGAATTACATTGA+AGG	-	chr2.4:10872432-10872451	MS.gene31507:CDS	35.0%
!	ATGGTGGAAAGAAAAATACG+TGG	-	chr2.4:10872175-10872194	MS.gene31507:CDS	35.0%
!	CCTTGATTATGCTAATTTGC+AGG	-	chr2.4:10872417-10872436	MS.gene31507:CDS	35.0%
!!	ATGGTCTTATAGGACTTGTT+AGG	-	chr2.4:10872272-10872291	MS.gene31507:CDS	35.0%
!!	GCATCAAAACATGGTCTTAT+AGG	-	chr2.4:10872262-10872281	MS.gene31507:CDS	35.0%
	ACACTGTACTAGCTAGAAAG+AGG	+	chr2.4:10871720-10871739	None:intergenic	40.0%
	CACTGTACTAGCTAGAAAGA+GGG	+	chr2.4:10871719-10871738	None:intergenic	40.0%
	GAGAGATGAGAAACAAGTTG+AGG	-	chr2.4:10871979-10871998	MS.gene31507:CDS	40.0%
	GTAAGGTAGCTATAGTGACT+GGG	-	chr2.4:10871810-10871829	MS.gene31507:CDS	40.0%
	GTGGCTTCTCAGTCATTAAT+CGG	-	chr2.4:10872536-10872555	MS.gene31507:intron	40.0%
	TAAGGTAGCTATAGTGACTG+GGG	-	chr2.4:10871811-10871830	MS.gene31507:CDS	40.0%
	TACACTGGCAGTGCAAATTA+TGG	+	chr2.4:10872203-10872222	None:intergenic	40.0%
!	CAACTTGTTTCTCATCTCTC+AGG	+	chr2.4:10871979-10871998	None:intergenic	40.0%
!!	CAGTGTCTACCTGTTTCTAT+TGG	-	chr2.4:10871734-10871753	MS.gene31507:CDS	40.0%
!!	GGAAATTGTTGCTTTCACGT+TGG	-	chr2.4:10872000-10872019	MS.gene31507:CDS	40.0%
	ACCAACAAAACCAGCTACAC+TGG	+	chr2.4:10872218-10872237	None:intergenic	45.0%
	GACATCATGTTCAGCAATGC+TGG	-	chr2.4:10872040-10872059	MS.gene31507:CDS	45.0%
	GCTTTCACGTTGGAGAGATA+TGG	-	chr2.4:10872010-10872029	MS.gene31507:CDS	45.0%
	GGACATAACTTGGTCGTTGA+TGG	-	chr2.4:10872514-10872533	MS.gene31507:intron	45.0%
	GGTAAGGTAGCTATAGTGAC+TGG	-	chr2.4:10871809-10871828	MS.gene31507:CDS	45.0%
!	AGAGGTGTAGCAACAGCATA+AGG	+	chr2.4:10872349-10872368	None:intergenic	45.0%
!	CATAACTTGGTCGTTGATGG+TGG	-	chr2.4:10872517-10872536	MS.gene31507:intron	45.0%
!	TGATCATAGGTTGGAGGGTA+AGG	-	chr2.4:10871793-10871812	MS.gene31507:CDS	45.0%
!	TTCAGCAATGCTGGTAATGC+TGG	-	chr2.4:10872049-10872068	MS.gene31507:CDS	45.0%
!!!	AGTGTAGCTGGTTTTGTTGG+TGG	-	chr2.4:10872217-10872236	MS.gene31507:CDS	45.0%
	ACCTCTTGCATGTAGAGCAC+TGG	-	chr2.4:10872363-10872382	MS.gene31507:CDS	50.0%
	AGGTCAACATGCAGTGAGCT+TGG	-	chr2.4:10872292-10872311	MS.gene31507:CDS	50.0%
	ATTTGCACTGCCAGTGTAGC+TGG	-	chr2.4:10872205-10872224	MS.gene31507:CDS	50.0%
	CAAGCATGCTGCACGTGTAA+TGG	-	chr2.4:10872156-10872175	MS.gene31507:CDS	50.0%
	CCAACAGCTTCGACTTCACT+GGG	+	chr2.4:10872397-10872416	None:intergenic	50.0%
	TCCAGTGCTCTACATGCAAG+AGG	+	chr2.4:10872367-10872386	None:intergenic	50.0%
	TCTCATCTCTCAGGTCACAG+TGG	+	chr2.4:10871970-10871989	None:intergenic	50.0%
	TGCAGTGAGCTTGGAGCTTA+TGG	-	chr2.4:10872301-10872320	MS.gene31507:CDS	50.0%
!!	TGCATGTAGAGCACTGGACA+TGG	-	chr2.4:10872369-10872388	MS.gene31507:CDS	50.0%
!!!	GCCAGTGTAGCTGGTTTTGT+TGG	-	chr2.4:10872214-10872233	MS.gene31507:CDS	50.0%
!!!	GGTTTTGTTGGTGGATGTGC+AGG	-	chr2.4:10872226-10872245	MS.gene31507:CDS	50.0%
	ACCACGAACGTTCACAGCCA+TGG	+	chr2.4:10872122-10872141	None:intergenic	55.0%
	ACCATGGCTGTGAACGTTCG+TGG	-	chr2.4:10872118-10872137	MS.gene31507:CDS	55.0%
	ATAGTGACTGGGGCAGCAAG+CGG	-	chr2.4:10871821-10871840	MS.gene31507:CDS	55.0%
	CATCTCTCAGGTCACAGTGG+TGG	+	chr2.4:10871967-10871986	None:intergenic	55.0%
	CCCAGTGAAGTCGAAGCTGT+TGG	-	chr2.4:10872394-10872413	MS.gene31507:CDS	55.0%
	GCATGCTGCACGTGTAATGG+TGG	-	chr2.4:10872159-10872178	MS.gene31507:CDS	55.0%
	GCCAACAGCTTCGACTTCAC+TGG	+	chr2.4:10872398-10872417	None:intergenic	55.0%
	ACTGGGGCAGCAAGCGGAAT+AGG	-	chr2.4:10871827-10871846	MS.gene31507:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	10871672	10872582	10871672	ID=MS.gene31507
chr2.4	mRNA	10871672	10872582	10871672	ID=MS.gene31507.t1;Parent=MS.gene31507
chr2.4	exon	10872569	10872582	10872569	ID=MS.gene31507.t1.exon1;Parent=MS.gene31507.t1
chr2.4	CDS	10872569	10872582	10872569	ID=cds.MS.gene31507.t1;Parent=MS.gene31507.t1
chr2.4	exon	10871672	10872452	10871672	ID=MS.gene31507.t1.exon2;Parent=MS.gene31507.t1
chr2.4	CDS	10871672	10872452	10871672	ID=cds.MS.gene31507.t1;Parent=MS.gene31507.t1

Gene Sequence

>MS.gene31507

ATGTCAAAACAAAGGTACGTATAACTTTGTATATTTATGAATCTCCCTCTTTCTAGCTAGTACAGTGTCTACCTGTTTCTATTGGCTTATAACAAATTAATGAATAGTATATTTTATTTTATGATCATAGGTTGGAGGGTAAGGTAGCTATAGTGACTGGGGCAGCAAGCGGAATAGGAGCAGAAGCAGTAAAGATATTCGTGGAAAATGGAGCATTTGTAGTCATAGCAGATATCAATGATGAATTGGGTCATCATGTTGCAAATTCAATAGGGTTAGACAAAGTGAGTTACCACCACTGTGACCTGAGAGATGAGAAACAAGTTGAGGAAATTGTTGCTTTCACGTTGGAGAGATATGGTACCTTAGACATCATGTTCAGCAATGCTGGTAATGCTGGTCCTTTATCTAGCATACTAGAATTTGACCTAAATGAATTTGACAACACCATGGCTGTGAACGTTCGTGGTGCAGCTGCAACTATCAAGCATGCTGCACGTGTAATGGTGGAAAGAAAAATACGTGGATCCATAATTTGCACTGCCAGTGTAGCTGGTTTTGTTGGTGGATGTGCAGGACATGATTATACAGCATCAAAACATGGTCTTATAGGACTTGTTAGGTCAACATGCAGTGAGCTTGGAGCTTATGGAATCAGAGTCAATTCAATCTCACCTTATGCTGTTGCTACACCTCTTGCATGTAGAGCACTGGACATGGTGCCCAGTGAAGTCGAAGCTGTTGGCCTTGATTATGCTAATTTGCAGGGAATTACATTGAAGGCTGCTCATATTGCAGAGACTGCTTTGTTTCTTGCATCTGATGAATCTGCTTATATTAGTGGACATAACTTGGTCGTTGATGGTGGCTTCTCAGTCATTAATCGGTGTCTTCCTAGTATTAAAAAATGA

Protein sequence

>MS.gene31507.t1

MSKQRLEGKVAIVTGAASGIGAEAVKIFVENGAFVVIADINDELGHHVANSIGLDKVSYHHCDLRDEKQVEEIVAFTLERYGTLDIMFSNAGNAGPLSSILEFDLNEFDNTMAVNVRGAAATIKHAARVMVERKIRGSIICTASVAGFVGGCAGHDYTASKHGLIGLVRSTCSELGAYGIRVNSISPYAVATPLACRALDMVPSEVEAVGLDYANLQGITLKAAHIAETALFLASDESAYISGHNLVVDGGFSVINRCLPSIKK