AlfalfaGEDB | MS.gene28848

M. sativa cultivar XinJiangDaYe / MS.gene28848

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28848.t1	AES82245.1	97.7	265	6	0	1	265	1	265	3.70E-139	504.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28848.t1	Q94K41	65.2	256	89	0	1	256	1	256	4.1e-91	335.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28848.t1	G7KTR5	97.7	265	6	0	1	265	1	265	2.6e-139	504.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050431	MS.gene28848	0.950007	3.99E-108	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene42260	MS.gene28848	PPI
MS.gene08694	MS.gene28848	PPI
MS.gene28848	MS.gene055565	PPI
MS.gene28848	MS.gene045581	PPI
MS.gene003926	MS.gene28848	PPI
MS.gene28848	MS.gene007678	PPI
MS.gene28848	MS.gene072462	PPI
MS.gene28848	MS.gene028938	PPI
MS.gene28848	MS.gene25667	PPI
MS.gene28848	MS.gene003992	PPI
MS.gene28848	MS.gene055846	PPI
MS.gene28848	MS.gene62195	PPI
MS.gene63484	MS.gene28848	PPI
MS.gene28848	MS.gene066728	PPI
MS.gene28848	MS.gene85724	PPI
MS.gene28848	MS.gene018066	PPI
MS.gene28848	MS.gene73824	PPI
MS.gene28848	MS.gene024269	PPI
MS.gene28848	MS.gene003729	PPI
MS.gene28848	MS.gene019786	PPI
MS.gene28848	MS.gene007002	PPI
MS.gene28848	MS.gene050131	PPI
MS.gene28848	MS.gene43481	PPI
MS.gene050431	MS.gene28848	PPI
MS.gene28848	MS.gene44088	PPI
MS.gene28848	MS.gene52743	PPI
MS.gene28848	MS.gene54598	PPI
MS.gene28848	MS.gene072664	PPI
MS.gene28848	MS.gene07097	PPI
MS.gene28848	MS.gene021164	PPI
MS.gene41122	MS.gene28848	PPI
MS.gene28848	MS.gene031693	PPI
MS.gene28848	MS.gene28847	PPI
MS.gene63308	MS.gene28848	PPI
MS.gene28848	MS.gene95020	PPI
MS.gene28848	MS.gene012681	PPI
MS.gene28848	MS.gene065380	PPI
MS.gene28848	MS.gene07096	PPI
MS.gene033685	MS.gene28848	PPI
MS.gene056029	MS.gene28848	PPI
MS.gene28848	MS.gene005221	PPI
MS.gene28848	MS.gene064756	PPI
MS.gene28848	MS.gene63483	PPI
MS.gene28848	MS.gene50524	PPI
MS.gene28848	MS.gene50522	PPI
MS.gene28848	MS.gene62187	PPI
MS.gene87026	MS.gene28848	PPI
MS.gene28848	MS.gene42856	PPI
MS.gene28848	MS.gene008162	PPI
MS.gene62193	MS.gene28848	PPI
MS.gene28848	MS.gene08694	PPI
MS.gene28848	MS.gene63482	PPI
MS.gene28848	MS.gene043387	PPI
MS.gene29327	MS.gene28848	PPI
MS.gene28848	MS.gene63403	PPI
MS.gene28848	MS.gene003925	PPI
MS.gene28848	MS.gene52742	PPI
MS.gene28848	MS.gene04700	PPI
MS.gene73088	MS.gene28848	PPI
MS.gene28848	MS.gene31507	PPI
MS.gene28848	MS.gene014425	PPI
MS.gene28848	MS.gene050862	PPI
MS.gene28848	MS.gene08695	PPI
MS.gene073684	MS.gene28848	PPI
MS.gene056339	MS.gene28848	PPI
MS.gene28848	MS.gene042142	PPI
MS.gene28848	MS.gene008161	PPI
MS.gene54952	MS.gene28848	PPI
MS.gene28848	MS.gene006999	PPI
MS.gene050432	MS.gene28848	PPI
MS.gene28848	MS.gene42114	PPI
MS.gene012794	MS.gene28848	PPI
MS.gene28848	MS.gene039828	PPI
MS.gene007006	MS.gene28848	PPI
MS.gene62194	MS.gene28848	PPI
MS.gene28848	MS.gene30551	PPI
MS.gene63307	MS.gene28848	PPI
MS.gene28848	MS.gene63306	PPI
MS.gene28848	MS.gene23019	PPI
MS.gene28848	MS.gene03319	PPI
MS.gene044694	MS.gene28848	PPI
MS.gene006617	MS.gene28848	PPI
MS.gene28848	MS.gene63300	PPI
MS.gene28848	MS.gene033685	PPI
MS.gene28848	MS.gene25674	PPI
MS.gene28848	MS.gene87780	PPI
MS.gene28848	MS.gene68880	PPI
MS.gene28848	MS.gene008153	PPI
MS.gene28848	MS.gene80360	PPI
MS.gene28848	MS.gene008160	PPI
MS.gene28848	MS.gene68956	PPI
MS.gene28848	MS.gene08756	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28848.t1	MTR_7g110140	97.736	265	6	0	1	265	1	265	0.0	522
MS.gene28848.t1	MTR_6g035395	71.765	255	59	1	3	257	2	243	3.07e-129	366
MS.gene28848.t1	MTR_7g110130	68.441	263	82	1	1	262	1	263	6.72e-128	363
MS.gene28848.t1	MTR_2g090165	67.816	261	83	1	1	260	1	261	1.21e-127	363
MS.gene28848.t1	MTR_7g110160	63.529	255	75	1	1	255	1	237	8.38e-109	314
MS.gene28848.t1	MTR_6g023590	50.394	254	124	2	2	253	9	262	3.31e-86	258
MS.gene28848.t1	MTR_6g023950	50.391	256	125	2	2	255	9	264	4.40e-83	250
MS.gene28848.t1	MTR_6g023600	48.425	254	129	2	2	253	9	262	9.05e-79	239
MS.gene28848.t1	MTR_6g023990	49.004	251	126	2	5	253	13	263	1.58e-78	238
MS.gene28848.t1	MTR_6g023610	50.000	250	123	2	5	252	12	261	7.73e-77	234
MS.gene28848.t1	MTR_4g052350	50.000	254	122	3	5	257	13	262	6.65e-76	232
MS.gene28848.t1	MTR_4g052340	50.000	254	122	3	5	257	13	262	1.08e-75	231
MS.gene28848.t1	MTR_4g052320	48.235	255	128	3	4	257	6	257	1.74e-75	231
MS.gene28848.t1	MTR_6g023600	48.031	254	124	3	2	253	9	256	3.12e-75	229
MS.gene28848.t1	MTR_7g105460	47.826	253	130	2	5	256	12	263	8.44e-75	229
MS.gene28848.t1	MTR_7g071680	46.014	276	127	4	2	257	25	298	1.68e-74	229
MS.gene28848.t1	MTR_6g023910	47.843	255	129	3	2	252	7	261	5.57e-73	224
MS.gene28848.t1	MTR_4g052300	47.843	255	128	3	4	257	6	256	2.69e-72	222
MS.gene28848.t1	MTR_7g083870	47.015	268	133	4	5	265	34	299	7.50e-71	220
MS.gene28848.t1	MTR_7g107670	43.494	269	144	4	2	264	29	295	2.17e-66	208
MS.gene28848.t1	MTR_3g020670	47.876	259	124	5	4	253	14	270	1.91e-62	197
MS.gene28848.t1	MTR_4g078630	45.802	262	129	5	1	253	14	271	4.10e-62	197
MS.gene28848.t1	MTR_7g105580	45.783	249	117	3	5	253	7	237	3.10e-61	194
MS.gene28848.t1	MTR_7g105590	46.739	184	96	2	5	187	7	189	3.76e-49	161
MS.gene28848.t1	MTR_7g105570	46.667	180	94	2	77	255	41	219	1.92e-45	152
MS.gene28848.t1	MTR_3g113870	42.857	182	101	3	14	194	29	208	2.76e-42	144
MS.gene28848.t1	MTR_4g084800	47.826	138	71	1	5	141	12	149	5.25e-38	131
MS.gene28848.t1	MTR_4g058840	30.798	263	153	5	6	251	11	261	2.11e-23	96.3
MS.gene28848.t1	MTR_4g058820	30.798	263	153	5	6	251	11	261	2.11e-23	96.3
MS.gene28848.t1	MTR_7g113650	29.845	258	164	6	4	251	38	288	2.81e-21	90.9
MS.gene28848.t1	MTR_1g051165	27.376	263	177	5	4	254	12	272	7.32e-20	87.0
MS.gene28848.t1	MTR_4g058810	35.450	189	115	3	6	187	11	199	7.78e-20	86.7
MS.gene28848.t1	MTR_3g085740	28.794	257	164	9	4	252	73	318	4.03e-19	85.5
MS.gene28848.t1	MTR_1g082760	29.213	267	154	7	4	251	38	288	9.34e-19	84.0
MS.gene28848.t1	MTR_4g131740	27.925	265	167	8	4	254	38	292	3.62e-18	82.4
MS.gene28848.t1	MTR_4g132850	29.730	259	155	7	5	252	6	248	4.70e-18	81.3
MS.gene28848.t1	MTR_6g023940	29.648	199	66	5	5	196	12	143	2.27e-17	77.4
MS.gene28848.t1	MTR_4g097510	31.217	189	126	4	7	194	3	188	3.76e-17	79.3
MS.gene28848.t1	MTR_1196s0010	30.040	253	156	9	7	252	275	513	6.78e-16	77.4
MS.gene28848.t1	MTR_8g442750	30.916	262	164	9	6	256	14	269	5.38e-15	73.6
MS.gene28848.t1	MTR_7g094520	29.297	256	165	7	6	252	11	259	8.27e-15	72.4
MS.gene28848.t1	MTR_8g096580	32.836	201	128	4	6	202	45	242	1.15e-14	72.8
MS.gene28848.t1	MTR_3g068085	28.958	259	174	9	6	260	15	267	1.54e-14	72.0
MS.gene28848.t1	MTR_3g068070	28.405	257	174	8	2	254	11	261	2.47e-14	71.2
MS.gene28848.t1	MTR_4g064887	27.376	263	169	9	2	254	11	261	9.36e-14	69.7
MS.gene28848.t1	MTR_7g105560	60.000	55	22	0	5	59	12	66	1.29e-13	67.0
MS.gene28848.t1	MTR_3g087850	30.939	181	115	5	10	187	30	203	1.84e-13	69.3
MS.gene28848.t1	MTR_7g094510	27.413	259	176	7	1	252	1	254	2.98e-13	68.2
MS.gene28848.t1	MTR_3g087860	30.939	181	113	6	28	203	34	207	2.80e-12	65.9
MS.gene28848.t1	MTR_4g066050	30.180	222	136	8	9	217	27	242	3.89e-12	65.1
MS.gene28848.t1	MTR_3g068065	26.357	258	182	6	6	260	17	269	5.19e-12	64.7
MS.gene28848.t1	MTR_4g086400	27.692	195	123	4	8	187	6	197	3.20e-11	63.5
MS.gene28848.t1	MTR_7g076660	36.264	91	54	1	9	95	8	98	9.47e-11	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28848.t1	AT2G47140	65.234	256	89	0	1	256	1	256	8.12e-122	348
MS.gene28848.t1	AT3G29250	61.479	257	97	2	1	256	1	256	9.80e-111	320
MS.gene28848.t1	AT3G29250	61.479	257	97	2	1	256	37	292	3.12e-110	320
MS.gene28848.t1	AT3G29250	61.176	255	97	2	3	256	41	294	4.80e-109	317
MS.gene28848.t1	AT3G29260	61.303	261	98	2	1	260	1	259	1.50e-108	314
MS.gene28848.t1	AT2G47130	60.000	260	95	2	1	256	1	255	1.50e-106	309
MS.gene28848.t1	AT2G47120	56.809	257	107	3	4	260	5	257	5.00e-100	293
MS.gene28848.t1	AT2G47120	56.154	260	110	3	1	260	1	256	7.74e-100	292
MS.gene28848.t1	AT3G51680	49.442	269	120	3	5	257	31	299	5.82e-76	233
MS.gene28848.t1	AT3G26760	41.791	268	143	5	5	261	35	300	1.65e-62	199
MS.gene28848.t1	AT1G52340	45.693	267	131	6	4	258	16	280	3.77e-62	197
MS.gene28848.t1	AT4G03140	42.481	266	145	4	5	264	77	340	2.17e-60	194
MS.gene28848.t1	AT3G26770	41.154	260	142	5	5	256	40	296	2.67e-60	193
MS.gene28848.t1	AT3G42960	43.956	273	135	5	2	265	4	267	2.41e-54	177
MS.gene28848.t1	AT2G47150	37.849	251	103	5	6	256	1	198	4.46e-43	145
MS.gene28848.t1	AT2G17845	30.769	273	151	10	6	255	47	304	1.08e-19	87.0
MS.gene28848.t1	AT2G29150	32.955	264	152	11	3	254	11	261	3.71e-19	84.7
MS.gene28848.t1	AT3G55290	30.189	265	152	8	6	251	17	267	4.46e-19	84.7
MS.gene28848.t1	AT3G55290	30.189	265	152	8	6	251	18	268	4.66e-19	84.7
MS.gene28848.t1	AT2G29360	30.769	260	162	10	3	254	13	262	1.37e-18	83.2
MS.gene28848.t1	AT2G29340	30.827	266	174	8	3	265	4	262	7.10e-18	81.3
MS.gene28848.t1	AT2G29340	30.980	255	168	7	3	254	4	253	1.06e-17	81.3
MS.gene28848.t1	AT1G62610	28.077	260	165	6	6	251	15	266	1.50e-17	80.5
MS.gene28848.t1	AT1G62610	28.077	260	165	6	6	251	19	270	1.63e-17	80.5
MS.gene28848.t1	AT1G62610	28.077	260	165	6	6	251	14	265	1.63e-17	80.5
MS.gene28848.t1	AT1G62610	28.077	260	165	6	6	251	13	264	1.89e-17	80.1
MS.gene28848.t1	AT3G55310	29.434	265	154	8	6	251	17	267	5.74e-17	79.0
MS.gene28848.t1	AT3G46170	29.344	259	162	8	6	251	26	276	1.10e-16	78.2
MS.gene28848.t1	AT1G63380	29.615	260	161	9	6	251	19	270	1.65e-16	77.8
MS.gene28848.t1	AT1G63380	29.615	260	161	9	6	251	15	266	1.69e-16	77.8
MS.gene28848.t1	AT1G63380	29.615	260	161	9	6	251	17	268	1.75e-16	77.8
MS.gene28848.t1	AT1G63380	29.615	260	161	9	6	251	22	273	2.13e-16	77.4
MS.gene28848.t1	AT1G24360	27.626	257	171	8	4	254	72	319	3.82e-16	77.0
MS.gene28848.t1	AT1G54870	29.344	259	164	7	4	251	82	332	6.74e-16	76.6
MS.gene28848.t1	AT1G54870	29.344	259	164	7	4	251	80	330	7.84e-16	76.3
MS.gene28848.t1	AT2G29370	29.615	260	165	10	3	254	13	262	1.76e-15	74.7
MS.gene28848.t1	AT3G05260	29.434	265	161	9	4	254	35	287	1.80e-15	74.7
MS.gene28848.t1	AT1G07450	28.405	257	175	7	1	254	3	253	2.53e-15	73.9
MS.gene28848.t1	AT2G29290	30.000	260	164	10	3	254	4	253	3.43e-15	73.6
MS.gene28848.t1	AT5G65205	30.256	195	126	5	1	192	1	188	2.11e-14	72.0
MS.gene28848.t1	AT2G30670	27.344	256	176	6	3	254	4	253	2.68e-14	71.2
MS.gene28848.t1	AT2G29350	29.119	261	166	10	2	254	11	260	3.54e-14	70.9
MS.gene28848.t1	AT2G29310	28.016	257	173	7	3	254	4	253	3.72e-14	70.9
MS.gene28848.t1	AT5G18210	29.615	260	166	8	4	252	6	259	3.82e-14	70.9
MS.gene28848.t1	AT1G07440	27.308	260	170	8	3	254	9	257	4.83e-14	70.5
MS.gene28848.t1	AT4G05530	30.189	265	146	8	5	252	8	250	7.50e-14	69.7
MS.gene28848.t1	AT5G10050	28.947	190	123	6	7	192	6	187	3.94e-13	68.2
MS.gene28848.t1	AT1G10310	30.366	191	128	4	9	196	18	206	4.46e-13	67.4
MS.gene28848.t1	AT2G29340	30.612	196	131	5	3	195	4	197	5.08e-13	66.6
MS.gene28848.t1	AT2G29340	30.612	196	131	5	3	195	4	197	5.08e-13	66.6
MS.gene28848.t1	AT5G06060	28.571	259	169	9	3	254	6	255	1.67e-12	66.2
MS.gene28848.t1	AT5G50600	26.210	248	158	8	6	232	45	288	2.22e-12	66.6
MS.gene28848.t1	AT5G50700	26.210	248	158	8	6	232	45	288	2.22e-12	66.6
MS.gene28848.t1	AT3G04000	29.545	264	160	10	6	251	14	269	2.40e-12	65.9
MS.gene28848.t1	AT4G20760	29.843	191	113	7	6	176	41	230	3.52e-12	65.5
MS.gene28848.t1	AT4G20760	29.843	191	113	7	6	176	61	250	3.82e-12	65.9
MS.gene28848.t1	AT3G12800	27.306	271	171	11	6	259	10	271	8.57e-12	64.3
MS.gene28848.t1	AT5G50770	30.978	184	119	5	8	187	47	226	5.58e-11	62.4
MS.gene28848.t1	AT4G13180	27.099	262	167	8	6	254	13	263	6.82e-11	61.6
MS.gene28848.t1	AT5G18210	31.188	202	126	6	4	194	6	205	7.35e-11	61.6

Find 51 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTGAAATTGTTTGCCGAAAA+TGG	0.162832	7.4:-5439803	MS.gene28848:CDS
AGTAGACATTCAAGATAAAT+TGG	0.192212	7.4:-5439765	MS.gene28848:CDS
TTTAGTAATGCAGGAATAAT+AGG	0.245500	7.4:-5439614	MS.gene28848:CDS
GTTAATAGTATATCCCCTTT+TGG	0.296854	7.4:-5439332	MS.gene28848:CDS
TGTGGAGAACTTGGTGGTTA+TGG	0.318822	7.4:-5439362	MS.gene28848:CDS
GAATGAATTTGAGAAAACTA+TGG	0.325001	7.4:-5439561	MS.gene28848:CDS
GGTCATAACTTGGTTGTTGA+TGG	0.345434	7.4:-5439149	MS.gene28848:CDS
GGAATAATAGGCTCTTTATC+TGG	0.363048	7.4:-5439602	MS.gene28848:CDS
ATGCAATTGGTCTACATATC+AGG	0.373432	7.4:+5439089	None:intergenic
TTTGAAGGGTATTGTGTTGA+AGG	0.381455	7.4:-5439231	MS.gene28848:CDS
TTTGATGTAGTATAACCATT+AGG	0.390831	7.4:+5439416	None:intergenic
ACAGGTGCAGCCAGTGGAAT+TGG	0.391122	7.4:-5439836	MS.gene28848:CDS
CATATCATCATTGTGATGTC+AGG	0.396278	7.4:-5439703	MS.gene28848:CDS
ACTAGCGTTGCAGCTTCTAT+TGG	0.399254	7.4:-5439449	MS.gene28848:CDS
ATTGGTGGAACAGGTCCTAA+TGG	0.415583	7.4:-5439431	MS.gene28848:CDS
AAAATCTAGGTTGGAGGGTA+AGG	0.417028	7.4:-5439870	MS.gene28848:CDS
GCAGCTTCTATTGGTGGAAC+AGG	0.426744	7.4:-5439440	MS.gene28848:CDS
GTCATAACTTGGTTGTTGAT+GGG	0.428146	7.4:-5439148	MS.gene28848:CDS
CGTAAAGTTGCAGATTCAAT+AGG	0.430870	7.4:-5439740	MS.gene28848:CDS
AGAGGTGTTGCAACTCCAAA+AGG	0.435975	7.4:+5439317	None:intergenic
AATATATTATGACAAAATCT+AGG	0.448610	7.4:-5439883	None:intergenic
TAGTTCTCATGCTAATTTGA+AGG	0.456961	7.4:-5439246	MS.gene28848:CDS
TTATATCAGTGGTCATAACT+TGG	0.462346	7.4:-5439159	MS.gene28848:CDS
GGTAAGGTGGCAATAATAAC+AGG	0.465773	7.4:-5439854	MS.gene28848:CDS
AGAAACAATTCATTTCACTT+TGG	0.465833	7.4:-5439663	MS.gene28848:CDS
TGTTTCTTCACCAATTCCAC+TGG	0.482489	7.4:+5439826	None:intergenic
TCATAACTTGGTTGTTGATG+GGG	0.490805	7.4:-5439147	MS.gene28848:CDS
TAAGCATGCTGCGCGCGCCA+TGG	0.496972	7.4:-5439507	MS.gene28848:CDS
GTAGACATTCAAGATAAATT+GGG	0.503523	7.4:-5439764	MS.gene28848:CDS
CATAACTTGGTTGTTGATGG+GGG	0.511840	7.4:-5439146	MS.gene28848:CDS
AGCGTTGCAGCTTCTATTGG+TGG	0.515248	7.4:-5439446	MS.gene28848:CDS
TACATATCAGGCGTTGCAGA+TGG	0.522877	7.4:+5439101	None:intergenic
AAATCAGCTTGTGGAGAACT+TGG	0.524096	7.4:-5439371	MS.gene28848:CDS
AGTTCTCATGCTAATTTGAA+GGG	0.524264	7.4:-5439245	MS.gene28848:CDS
TATTATGACAAAATCTAGGT+TGG	0.525544	7.4:-5439879	None:intergenic
GAGGTGTTGCAACTCCAAAA+GGG	0.538111	7.4:+5439318	None:intergenic
ACTATGGCTACAAATGTTGT+TGG	0.550336	7.4:-5439545	MS.gene28848:CDS
TATGACAAAATCTAGGTTGG+AGG	0.554009	7.4:-5439876	MS.gene28848:CDS
ATATCATCATTGTGATGTCA+GGG	0.558680	7.4:-5439702	MS.gene28848:CDS
GATGAAGCTGTTTATATCAG+TGG	0.560159	7.4:-5439170	MS.gene28848:CDS
ATGACAAAATCTAGGTTGGA+GGG	0.562378	7.4:-5439875	MS.gene28848:CDS
GGACTTGTGAAATCAGCTTG+TGG	0.562826	7.4:-5439380	MS.gene28848:CDS
TTGTAAGCTATACACGAAAG+AGG	0.567482	7.4:+5439299	None:intergenic
TCAGCTTGTGGAGAACTTGG+TGG	0.578232	7.4:-5439368	MS.gene28848:CDS
ATCTAGGTTGGAGGGTAAGG+TGG	0.582293	7.4:-5439867	MS.gene28848:CDS
GCATGCTGCGCGCGCCATGG+TGG	0.599712	7.4:-5439504	MS.gene28848:CDS
CTACTAGATTCAACTTCATG+AGG	0.605548	7.4:+5439269	None:intergenic
AGGTGTTGCAACTCCAAAAG+GGG	0.641177	7.4:+5439319	None:intergenic
ATAATAACAGGTGCAGCCAG+TGG	0.644904	7.4:-5439842	MS.gene28848:CDS
TAGTGCATATAATCGATCCA+CGG	0.666748	7.4:+5439471	None:intergenic
AGATGGAGTATTACGAACCA+CGG	0.715903	7.4:+5439118	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGAAACAATTCATTTCACTT+TGG	-	chr7.4:5439312-5439331	MS.gene28848:CDS	25.0%
!	AGTAGACATTCAAGATAAAT+TGG	-	chr7.4:5439210-5439229	MS.gene28848:CDS	25.0%
!	GAATGAATTTGAGAAAACTA+TGG	-	chr7.4:5439414-5439433	MS.gene28848:CDS	25.0%
!	GTAGACATTCAAGATAAATT+GGG	-	chr7.4:5439211-5439230	MS.gene28848:CDS	25.0%
!	TTTAGTAATGCAGGAATAAT+AGG	-	chr7.4:5439361-5439380	MS.gene28848:CDS	25.0%
!	TTTGATGTAGTATAACCATT+AGG	+	chr7.4:5439562-5439581	None:intergenic	25.0%
!!	AATAACAAATGCTCCATTTT+CGG	+	chr7.4:5439188-5439207	None:intergenic	25.0%
!!!	GATATTTTGTTTAGTAATGC+AGG	-	chr7.4:5439352-5439371	MS.gene28848:CDS	25.0%
	AGTTCTCATGCTAATTTGAA+GGG	-	chr7.4:5439730-5439749	MS.gene28848:CDS	30.0%
	ATATCATCATTGTGATGTCA+GGG	-	chr7.4:5439273-5439292	MS.gene28848:CDS	30.0%
	TAGTTCTCATGCTAATTTGA+AGG	-	chr7.4:5439729-5439748	MS.gene28848:CDS	30.0%
	TTATATCAGTGGTCATAACT+TGG	-	chr7.4:5439816-5439835	MS.gene28848:CDS	30.0%
!	ACATCAAAACATGCTCTTTT+AGG	-	chr7.4:5439574-5439593	MS.gene28848:CDS	30.0%
!	CATTTCACTTTGGAAAAACA+CGG	-	chr7.4:5439322-5439341	MS.gene28848:CDS	30.0%
!	GTTAATAGTATATCCCCTTT+TGG	-	chr7.4:5439643-5439662	MS.gene28848:CDS	30.0%
	ACTATGGCTACAAATGTTGT+TGG	-	chr7.4:5439430-5439449	MS.gene28848:CDS	35.0%
	CATATCATCATTGTGATGTC+AGG	-	chr7.4:5439272-5439291	MS.gene28848:CDS	35.0%
	CGTAAAGTTGCAGATTCAAT+AGG	-	chr7.4:5439235-5439254	MS.gene28848:CDS	35.0%
	CTACTAGATTCAACTTCATG+AGG	+	chr7.4:5439709-5439728	None:intergenic	35.0%
	GATGAAGCTGTTTATATCAG+TGG	-	chr7.4:5439805-5439824	MS.gene28848:CDS	35.0%
	GGAATAATAGGCTCTTTATC+TGG	-	chr7.4:5439373-5439392	MS.gene28848:CDS	35.0%
	GTCATAACTTGGTTGTTGAT+GGG	-	chr7.4:5439827-5439846	MS.gene28848:CDS	35.0%
	GTGAAATTGTTTGCCGAAAA+TGG	-	chr7.4:5439172-5439191	MS.gene28848:CDS	35.0%
	TAGTGCATATAATCGATCCA+CGG	+	chr7.4:5439507-5439526	None:intergenic	35.0%
	TCATAACTTGGTTGTTGATG+GGG	-	chr7.4:5439828-5439847	MS.gene28848:CDS	35.0%
	TTGTAAGCTATACACGAAAG+AGG	+	chr7.4:5439679-5439698	None:intergenic	35.0%
!	ATGACAAAATCTAGGTTGGA+GGG	-	chr7.4:5439100-5439119	MS.gene28848:CDS	35.0%
!!	TTTGAAGGGTATTGTGTTGA+AGG	-	chr7.4:5439744-5439763	MS.gene28848:CDS	35.0%
	AAATCAGCTTGTGGAGAACT+TGG	-	chr7.4:5439604-5439623	MS.gene28848:CDS	40.0%
	ATGGTGGCTAAAAAAATCCG+TGG	-	chr7.4:5439487-5439506	MS.gene28848:CDS	40.0%
	GGTAAGGTGGCAATAATAAC+AGG	-	chr7.4:5439121-5439140	MS.gene28848:CDS	40.0%
	GGTCATAACTTGGTTGTTGA+TGG	-	chr7.4:5439826-5439845	MS.gene28848:CDS	40.0%
	TGATGTCAGGGATGAAAAAC+AGG	-	chr7.4:5439285-5439304	MS.gene28848:CDS	40.0%
	TGTTTCTTCACCAATTCCAC+TGG	+	chr7.4:5439152-5439171	None:intergenic	40.0%
!	AAAATCTAGGTTGGAGGGTA+AGG	-	chr7.4:5439105-5439124	MS.gene28848:CDS	40.0%
!	AGATGGAGTATTACGAACCA+CGG	+	chr7.4:5439860-5439879	None:intergenic	40.0%
!	CATAACTTGGTTGTTGATGG+GGG	-	chr7.4:5439829-5439848	MS.gene28848:CDS	40.0%
!!!	ACGGATTTTTTTAGCCACCA+TGG	+	chr7.4:5439488-5439507	None:intergenic	40.0%
	ACTAGCGTTGCAGCTTCTAT+TGG	-	chr7.4:5439526-5439545	MS.gene28848:CDS	45.0%
	ATAATAACAGGTGCAGCCAG+TGG	-	chr7.4:5439133-5439152	MS.gene28848:CDS	45.0%
	ATTGGTGGAACAGGTCCTAA+TGG	-	chr7.4:5439544-5439563	MS.gene28848:CDS	45.0%
	GGACTTGTGAAATCAGCTTG+TGG	-	chr7.4:5439595-5439614	MS.gene28848:CDS	45.0%
!	AGGTGTTGCAACTCCAAAAG+GGG	+	chr7.4:5439659-5439678	None:intergenic	45.0%
!	TACATATCAGGCGTTGCAGA+TGG	+	chr7.4:5439877-5439896	None:intergenic	45.0%
!	TGTGGAGAACTTGGTGGTTA+TGG	-	chr7.4:5439613-5439632	MS.gene28848:CDS	45.0%
!!	AGAGGTGTTGCAACTCCAAA+AGG	+	chr7.4:5439661-5439680	None:intergenic	45.0%
!!	GAGGTGTTGCAACTCCAAAA+GGG	+	chr7.4:5439660-5439679	None:intergenic	45.0%
	AGCGTTGCAGCTTCTATTGG+TGG	-	chr7.4:5439529-5439548	MS.gene28848:CDS	50.0%
	GCAGCTTCTATTGGTGGAAC+AGG	-	chr7.4:5439535-5439554	MS.gene28848:CDS	50.0%
	TCAGCTTGTGGAGAACTTGG+TGG	-	chr7.4:5439607-5439626	MS.gene28848:CDS	50.0%
!	ATCTAGGTTGGAGGGTAAGG+TGG	-	chr7.4:5439108-5439127	MS.gene28848:CDS	50.0%
	ACAGGTGCAGCCAGTGGAAT+TGG	-	chr7.4:5439139-5439158	MS.gene28848:CDS	55.0%
!	TGTTGATGGGGGTTTCTCCG+TGG	-	chr7.4:5439840-5439859	MS.gene28848:CDS	55.0%
	TAAGCATGCTGCGCGCGCCA+TGG	-	chr7.4:5439468-5439487	MS.gene28848:CDS	65.0%
	GCATGCTGCGCGCGCCATGG+TGG	-	chr7.4:5439471-5439490	MS.gene28848:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	5439100	5439897	5439100	ID=MS.gene28848
chr7.4	mRNA	5439100	5439897	5439100	ID=MS.gene28848.t1;Parent=MS.gene28848
chr7.4	exon	5439100	5439897	5439100	ID=MS.gene28848.t1.exon1;Parent=MS.gene28848.t1
chr7.4	CDS	5439100	5439897	5439100	ID=cds.MS.gene28848.t1;Parent=MS.gene28848.t1

Gene Sequence

>MS.gene28848

ATGACAAAATCTAGGTTGGAGGGTAAGGTGGCAATAATAACAGGTGCAGCCAGTGGAATTGGTGAAGAAACAGTGAAATTGTTTGCCGAAAATGGAGCATTTGTTATTGCAGTAGACATTCAAGATAAATTGGGTCGTAAAGTTGCAGATTCAATAGGCTCAGATAAAGTAACATATCATCATTGTGATGTCAGGGATGAAAAACAGGTCGAAGAAACAATTCATTTCACTTTGGAAAAACACGGTTGCATCGATATTTTGTTTAGTAATGCAGGAATAATAGGCTCTTTATCTGGAATACTTGATCTTGATTTGAATGAATTTGAGAAAACTATGGCTACAAATGTTGTTGGTGCAGCTGCTACAATTAAGCATGCTGCGCGCGCCATGGTGGCTAAAAAAATCCGTGGATCGATTATATGCACTACTAGCGTTGCAGCTTCTATTGGTGGAACAGGTCCTAATGGTTATACTACATCAAAACATGCTCTTTTAGGACTTGTGAAATCAGCTTGTGGAGAACTTGGTGGTTATGGAATTAGAGTTAATAGTATATCCCCTTTTGGAGTTGCAACACCTCTTTCGTGTATAGCTTACAATCTTGAACCTCATGAAGTTGAATCTAGTAGTAGTTCTCATGCTAATTTGAAGGGTATTGTGTTGAAGGCTAAGCATGTTGCAGAAGCAGCTTTGTTTCTTGCTTCTGATGAAGCTGTTTATATCAGTGGTCATAACTTGGTTGTTGATGGGGGTTTCTCCGTGGTTCGTAATACTCCATCTGCAACGCCTGATATGTAG

Protein sequence

>MS.gene28848.t1

MTKSRLEGKVAIITGAASGIGEETVKLFAENGAFVIAVDIQDKLGRKVADSIGSDKVTYHHCDVRDEKQVEETIHFTLEKHGCIDILFSNAGIIGSLSGILDLDLNEFEKTMATNVVGAAATIKHAARAMVAKKIRGSIICTTSVAASIGGTGPNGYTTSKHALLGLVKSACGELGGYGIRVNSISPFGVATPLSCIAYNLEPHEVESSSSSHANLKGIVLKAKHVAEAALFLASDEAVYISGHNLVVDGGFSVVRNTPSATPDM