AlfalfaGEDB | MS.gene072664

M. sativa cultivar XinJiangDaYe / MS.gene072664

KEGG: -
GO: -
PFAM: PF13561.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072664.t1	XP_003626027.2	98.1	264	5	0	1	264	2	265	2.10E-139	505

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072664.t1	Q94K41	65.6	253	87	0	3	255	4	256	7.0e-91	335.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072664.t1	A0A396H746	98.1	264	5	0	1	264	2	265	1.5e-139	505.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050720	MS.gene072664	0.8021	6.58E-49	-1.69E-46
MS.gene050725	MS.gene072664	0.806351	8.58E-50	-1.69E-46
MS.gene051044	MS.gene072664	0.82338	1.43E-53	-1.69E-46
MS.gene051138	MS.gene072664	0.808318	3.29E-50	-1.69E-46
MS.gene051440	MS.gene072664	0.808007	3.83E-50	-1.69E-46
MS.gene051517	MS.gene072664	0.810174	1.31E-50	-1.69E-46
MS.gene052015	MS.gene072664	0.823981	1.03E-53	-1.69E-46
MS.gene052494	MS.gene072664	0.817292	3.56E-52	-1.69E-46
MS.gene052606	MS.gene072664	0.800911	1.15E-48	-1.69E-46
MS.gene055577	MS.gene072664	-0.803331	3.67E-49	-1.69E-46
MS.gene05589	MS.gene072664	-0.813698	2.24E-51	-1.69E-46
MS.gene056856	MS.gene072664	0.806576	7.69E-50	-1.69E-46
MS.gene057768	MS.gene072664	0.81023	1.28E-50	-1.69E-46
MS.gene05837	MS.gene072664	-0.803918	2.77E-49	-1.69E-46
MS.gene05960	MS.gene072664	0.837544	4.88E-57	-1.69E-46
MS.gene060405	MS.gene072664	0.801089	1.06E-48	-1.69E-46
MS.gene061044	MS.gene072664	0.806814	6.85E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene056029	MS.gene072664	PPI
MS.gene021164	MS.gene072664	PPI
MS.gene072664	MS.gene039828	PPI
MS.gene050862	MS.gene072664	PPI
MS.gene63483	MS.gene072664	PPI
MS.gene045581	MS.gene072664	PPI
MS.gene072664	MS.gene30551	PPI
MS.gene050431	MS.gene072664	PPI
MS.gene072664	MS.gene23019	PPI
MS.gene63403	MS.gene072664	PPI
MS.gene019786	MS.gene072664	PPI
MS.gene072664	MS.gene03319	PPI
MS.gene003925	MS.gene072664	PPI
MS.gene072664	MS.gene63300	PPI
MS.gene066728	MS.gene072664	PPI
MS.gene072664	MS.gene25674	PPI
MS.gene072664	MS.gene87780	PPI
MS.gene85724	MS.gene072664	PPI
MS.gene072664	MS.gene68880	PPI
MS.gene072664	MS.gene008153	PPI
MS.gene41122	MS.gene072664	PPI
MS.gene63308	MS.gene072664	PPI
MS.gene072664	MS.gene80360	PPI
MS.gene072664	MS.gene008160	PPI
MS.gene072664	MS.gene08756	PPI
MS.gene033685	MS.gene072664	PPI
MS.gene072664	MS.gene055565	PPI
MS.gene072664	MS.gene007678	PPI
MS.gene072664	MS.gene072462	PPI
MS.gene072664	MS.gene028938	PPI
MS.gene072664	MS.gene25667	PPI
MS.gene018066	MS.gene072664	PPI
MS.gene28848	MS.gene072664	PPI
MS.gene072664	MS.gene003992	PPI
MS.gene87026	MS.gene072664	PPI
MS.gene68956	MS.gene072664	PPI
MS.gene072664	MS.gene055846	PPI
MS.gene072664	MS.gene62195	PPI
MS.gene62193	MS.gene072664	PPI
MS.gene29327	MS.gene072664	PPI
MS.gene73088	MS.gene072664	PPI
MS.gene072664	MS.gene018066	PPI
MS.gene072664	MS.gene73824	PPI
MS.gene073684	MS.gene072664	PPI
MS.gene056339	MS.gene072664	PPI
MS.gene07096	MS.gene072664	PPI
MS.gene54952	MS.gene072664	PPI
MS.gene031693	MS.gene072664	PPI
MS.gene072664	MS.gene024269	PPI
MS.gene072664	MS.gene003729	PPI
MS.gene050432	MS.gene072664	PPI
MS.gene072664	MS.gene007002	PPI
MS.gene072664	MS.gene050131	PPI
MS.gene072664	MS.gene43481	PPI
MS.gene42260	MS.gene072664	PPI
MS.gene072664	MS.gene44088	PPI
MS.gene012794	MS.gene072664	PPI
MS.gene52742	MS.gene072664	PPI
MS.gene007006	MS.gene072664	PPI
MS.gene072664	MS.gene07097	PPI
MS.gene28847	MS.gene072664	PPI
MS.gene62194	MS.gene072664	PPI
MS.gene072664	MS.gene31507	PPI
MS.gene63307	MS.gene072664	PPI
MS.gene044694	MS.gene072664	PPI
MS.gene072664	MS.gene95020	PPI
MS.gene072664	MS.gene065380	PPI
MS.gene006617	MS.gene072664	PPI
MS.gene012681	MS.gene072664	PPI
MS.gene54598	MS.gene072664	PPI
MS.gene008162	MS.gene072664	PPI
MS.gene072664	MS.gene50524	PPI
MS.gene065380	MS.gene072664	PPI
MS.gene08694	MS.gene072664	PPI
MS.gene064756	MS.gene072664	PPI
MS.gene42856	MS.gene072664	PPI
MS.gene003926	MS.gene072664	PPI
MS.gene52743	MS.gene072664	PPI
MS.gene072664	MS.gene63482	PPI
MS.gene50522	MS.gene072664	PPI
MS.gene072664	MS.gene043387	PPI
MS.gene072664	MS.gene52742	PPI
MS.gene072664	MS.gene04700	PPI
MS.gene072664	MS.gene014425	PPI
MS.gene007678	MS.gene072664	PPI
MS.gene072664	MS.gene08695	PPI
MS.gene072664	MS.gene042142	PPI
MS.gene63306	MS.gene072664	PPI
MS.gene63484	MS.gene072664	PPI
MS.gene62187	MS.gene072664	PPI
MS.gene072664	MS.gene008161	PPI
MS.gene072664	MS.gene006999	PPI
MS.gene005221	MS.gene072664	PPI
MS.gene072664	MS.gene42114	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072664.t1	MTR_7g110140	97.719	263	6	0	2	264	3	265	0.0	517
MS.gene072664.t1	MTR_6g035395	72.266	256	58	1	1	256	1	243	6.61e-131	370
MS.gene072664.t1	MTR_2g090165	68.340	259	81	1	2	259	3	261	2.56e-127	362
MS.gene072664.t1	MTR_7g110130	68.582	261	81	1	2	261	3	263	3.41e-127	362
MS.gene072664.t1	MTR_7g110160	64.032	253	73	1	2	254	3	237	1.05e-108	314
MS.gene072664.t1	MTR_6g023590	50.598	251	122	2	4	252	12	262	6.84e-86	257
MS.gene072664.t1	MTR_6g023950	50.593	253	123	2	4	254	12	264	8.25e-83	249
MS.gene072664.t1	MTR_6g023990	49.004	251	126	2	4	252	13	263	1.75e-78	238
MS.gene072664.t1	MTR_6g023600	48.606	251	127	2	4	252	12	262	2.28e-78	238
MS.gene072664.t1	MTR_6g023610	50.000	250	123	2	4	251	12	261	7.63e-77	234
MS.gene072664.t1	MTR_4g052350	49.804	255	123	3	3	256	12	262	3.42e-76	233
MS.gene072664.t1	MTR_4g052340	49.804	255	123	3	3	256	12	262	5.57e-76	232
MS.gene072664.t1	MTR_4g052320	48.425	254	127	3	4	256	7	257	2.41e-75	230
MS.gene072664.t1	MTR_6g023600	48.207	251	122	3	4	252	12	256	7.60e-75	229
MS.gene072664.t1	MTR_7g071680	46.350	274	125	4	3	256	27	298	7.60e-75	230
MS.gene072664.t1	MTR_7g105460	47.826	253	130	2	4	255	12	263	8.06e-75	229
MS.gene072664.t1	MTR_6g023910	47.600	250	129	2	4	251	12	261	5.75e-73	224
MS.gene072664.t1	MTR_4g052300	48.031	254	127	3	4	256	7	256	4.09e-72	222
MS.gene072664.t1	MTR_7g083870	47.015	268	133	4	3	263	33	298	4.74e-71	220
MS.gene072664.t1	MTR_7g107670	43.446	267	143	4	3	263	31	295	2.84e-66	208
MS.gene072664.t1	MTR_3g020670	48.263	259	123	5	3	252	14	270	2.16e-63	200
MS.gene072664.t1	MTR_4g078630	46.332	259	126	5	3	252	17	271	2.60e-62	197
MS.gene072664.t1	MTR_7g105580	45.600	250	118	3	3	252	6	237	1.46e-61	194
MS.gene072664.t1	MTR_7g105590	46.486	185	97	2	3	186	6	189	2.03e-49	161
MS.gene072664.t1	MTR_7g105570	38.645	251	101	3	5	254	21	219	1.93e-45	152
MS.gene072664.t1	MTR_3g113870	42.857	182	101	3	13	193	29	208	2.59e-42	144
MS.gene072664.t1	MTR_4g084800	46.809	141	74	1	1	140	9	149	2.79e-38	131
MS.gene072664.t1	MTR_4g058840	30.798	263	153	5	5	250	11	261	2.00e-23	96.3
MS.gene072664.t1	MTR_4g058820	30.798	263	153	5	5	250	11	261	2.00e-23	96.3
MS.gene072664.t1	MTR_7g113650	29.961	257	163	6	4	250	39	288	2.98e-21	90.9
MS.gene072664.t1	MTR_1g051165	27.757	263	176	5	3	253	12	272	7.61e-21	89.7
MS.gene072664.t1	MTR_3g085740	29.183	257	163	9	3	251	73	318	4.74e-20	88.2
MS.gene072664.t1	MTR_4g058810	35.450	189	115	3	5	186	11	199	7.33e-20	86.7
MS.gene072664.t1	MTR_1g082760	29.323	266	153	7	4	250	39	288	1.05e-18	84.0
MS.gene072664.t1	MTR_4g132850	29.389	262	158	7	1	251	3	248	2.67e-18	82.0
MS.gene072664.t1	MTR_4g131740	28.030	264	166	8	4	253	39	292	4.65e-18	82.0
MS.gene072664.t1	MTR_6g023940	29.648	199	66	5	4	195	12	143	2.71e-17	77.0
MS.gene072664.t1	MTR_4g097510	31.217	189	126	4	6	193	3	188	3.28e-17	79.7
MS.gene072664.t1	MTR_1196s0010	30.040	253	156	9	6	251	275	513	7.11e-16	77.4
MS.gene072664.t1	MTR_8g442750	30.916	262	164	9	5	255	14	269	4.35e-15	73.9
MS.gene072664.t1	MTR_8g096580	32.512	203	130	4	3	201	43	242	7.22e-15	73.6
MS.gene072664.t1	MTR_7g094520	29.297	256	165	7	5	251	11	259	7.90e-15	72.8
MS.gene072664.t1	MTR_3g068085	28.958	259	174	9	5	259	15	267	1.27e-14	72.0
MS.gene072664.t1	MTR_3g068070	28.516	256	173	8	2	253	12	261	1.98e-14	71.6
MS.gene072664.t1	MTR_7g105560	56.897	58	25	0	1	58	9	66	7.23e-14	67.8
MS.gene072664.t1	MTR_4g064887	27.481	262	168	9	2	253	12	261	8.87e-14	69.7
MS.gene072664.t1	MTR_3g087850	30.939	181	115	5	9	186	30	203	1.87e-13	69.3
MS.gene072664.t1	MTR_7g094510	27.559	254	172	7	5	251	6	254	2.69e-13	68.2
MS.gene072664.t1	MTR_3g087860	30.939	181	113	6	27	202	34	207	2.87e-12	65.5
MS.gene072664.t1	MTR_4g066050	30.180	222	136	8	8	216	27	242	3.62e-12	65.1
MS.gene072664.t1	MTR_3g068065	26.357	258	182	6	5	259	17	269	4.69e-12	65.1
MS.gene072664.t1	MTR_4g086400	27.692	195	123	4	7	186	6	197	2.77e-11	63.9
MS.gene072664.t1	MTR_7g063270	27.626	257	175	7	2	252	13	264	7.13e-11	61.6
MS.gene072664.t1	MTR_7g076660	36.264	91	54	1	8	94	8	98	8.84e-11	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072664.t1	AT2G47140	65.613	253	87	0	3	255	4	256	3.90e-121	346
MS.gene072664.t1	AT3G29250	62.055	253	94	2	4	255	5	256	2.58e-110	319
MS.gene072664.t1	AT3G29250	61.328	256	97	2	1	255	40	294	3.47e-110	320
MS.gene072664.t1	AT3G29250	62.055	253	94	2	4	255	41	292	8.04e-110	319
MS.gene072664.t1	AT3G29260	62.016	258	95	2	3	259	4	259	1.21e-108	315
MS.gene072664.t1	AT2G47130	60.547	256	92	2	4	255	5	255	4.28e-106	308
MS.gene072664.t1	AT2G47120	56.641	256	107	3	4	259	5	256	2.37e-99	291
MS.gene072664.t1	AT2G47120	56.641	256	107	3	4	259	6	257	2.48e-99	291
MS.gene072664.t1	AT3G51680	48.519	270	123	3	3	256	30	299	3.37e-76	234
MS.gene072664.t1	AT1G52340	46.067	267	130	6	3	257	16	280	4.87e-63	199
MS.gene072664.t1	AT3G26760	41.636	269	144	5	3	260	34	300	8.59e-63	199
MS.gene072664.t1	AT3G26770	40.996	261	143	5	3	255	39	296	1.33e-60	194
MS.gene072664.t1	AT4G03140	42.322	267	146	4	3	263	76	340	1.36e-60	195
MS.gene072664.t1	AT3G42960	44.280	271	133	5	3	264	6	267	1.30e-54	177
MS.gene072664.t1	AT2G47150	37.849	251	103	5	5	255	1	198	5.80e-43	145
MS.gene072664.t1	AT2G17845	30.147	272	154	8	5	254	47	304	1.13e-19	87.0
MS.gene072664.t1	AT3G55290	30.189	265	152	8	5	250	18	268	4.39e-19	84.7
MS.gene072664.t1	AT3G55290	30.189	265	152	8	5	250	17	267	4.69e-19	84.7
MS.gene072664.t1	AT2G29150	32.061	262	155	10	2	253	13	261	5.51e-19	84.3
MS.gene072664.t1	AT2G29360	30.769	260	162	10	2	253	13	262	1.15e-18	83.6
MS.gene072664.t1	AT2G29340	30.827	266	174	8	2	264	4	262	3.80e-18	82.0
MS.gene072664.t1	AT2G29340	30.980	255	168	7	2	253	4	253	6.94e-18	82.0
MS.gene072664.t1	AT1G62610	28.077	260	165	6	5	250	19	270	1.45e-17	80.5
MS.gene072664.t1	AT1G62610	28.077	260	165	6	5	250	13	264	1.49e-17	80.5
MS.gene072664.t1	AT1G62610	28.077	260	165	6	5	250	14	265	1.65e-17	80.5
MS.gene072664.t1	AT1G62610	28.077	260	165	6	5	250	15	266	1.74e-17	80.5
MS.gene072664.t1	AT3G55310	29.434	265	154	8	5	250	17	267	5.20e-17	79.0
MS.gene072664.t1	AT1G24360	28.016	257	170	8	3	253	72	319	5.70e-17	79.3
MS.gene072664.t1	AT3G46170	29.344	259	162	8	5	250	26	276	1.34e-16	78.2
MS.gene072664.t1	AT1G63380	29.615	260	161	9	5	250	19	270	1.56e-16	77.8
MS.gene072664.t1	AT1G63380	29.615	260	161	9	5	250	17	268	1.60e-16	77.8
MS.gene072664.t1	AT1G63380	29.615	260	161	9	5	250	15	266	1.62e-16	77.8
MS.gene072664.t1	AT1G63380	29.615	260	161	9	5	250	22	273	2.01e-16	77.4
MS.gene072664.t1	AT1G54870	29.457	258	163	7	4	250	83	332	8.21e-16	76.3
MS.gene072664.t1	AT1G54870	29.457	258	163	7	4	250	81	330	8.41e-16	76.3
MS.gene072664.t1	AT2G29370	29.615	260	165	10	2	253	13	262	1.06e-15	75.1
MS.gene072664.t1	AT3G05260	29.545	264	160	9	4	253	36	287	2.18e-15	74.7
MS.gene072664.t1	AT1G07450	28.627	255	173	7	2	253	5	253	2.28e-15	74.3
MS.gene072664.t1	AT2G29290	30.000	260	164	10	2	253	4	253	2.34e-15	74.3
MS.gene072664.t1	AT2G30670	27.344	256	176	6	2	253	4	253	1.60e-14	72.0
MS.gene072664.t1	AT2G29310	28.016	257	173	7	2	253	4	253	2.26e-14	71.2
MS.gene072664.t1	AT2G29350	29.231	260	165	10	2	253	12	260	2.75e-14	71.2
MS.gene072664.t1	AT4G05530	29.851	268	149	8	1	251	5	250	2.78e-14	70.9
MS.gene072664.t1	AT5G65205	30.159	189	122	5	6	191	7	188	2.84e-14	71.6
MS.gene072664.t1	AT1G07440	27.308	260	170	8	2	253	9	257	2.88e-14	71.2
MS.gene072664.t1	AT5G18210	29.457	258	165	8	5	251	8	259	8.10e-14	69.7
MS.gene072664.t1	AT2G29340	30.612	196	131	5	2	194	4	197	3.10e-13	67.4
MS.gene072664.t1	AT2G29340	30.612	196	131	5	2	194	4	197	3.10e-13	67.4
MS.gene072664.t1	AT5G10050	28.947	190	123	6	6	191	6	187	3.32e-13	68.2
MS.gene072664.t1	AT1G10310	30.366	191	128	4	8	195	18	206	4.14e-13	67.4
MS.gene072664.t1	AT5G06060	28.571	259	169	9	2	253	6	255	1.16e-12	66.6
MS.gene072664.t1	AT5G50600	26.000	250	160	8	3	231	43	288	1.25e-12	67.4
MS.gene072664.t1	AT5G50700	26.000	250	160	8	3	231	43	288	1.25e-12	67.4
MS.gene072664.t1	AT3G04000	29.545	264	160	10	5	250	14	269	2.27e-12	65.9
MS.gene072664.t1	AT4G20760	29.843	191	113	7	5	175	61	250	3.66e-12	65.9
MS.gene072664.t1	AT4G20760	29.843	191	113	7	5	175	41	230	4.25e-12	65.5
MS.gene072664.t1	AT3G12800	27.306	271	171	11	5	258	10	271	8.54e-12	64.3
MS.gene072664.t1	AT5G50770	30.000	190	125	5	1	186	41	226	4.83e-11	62.4
MS.gene072664.t1	AT4G13180	27.099	262	167	8	5	253	13	263	6.87e-11	61.6

Find 48 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTGAAATTGTTTGCCGAAAA+TGG	0.162832	7.3:-5004415	MS.gene072664:CDS
AGTAGACATTCAAGATAAAT+TGG	0.192212	7.3:-5004377	MS.gene072664:CDS
TTTAGTAATGCAGGAATAAT+AGG	0.245500	7.3:-5004226	MS.gene072664:CDS
GTTAATAGTATATCCCCTTT+TGG	0.296854	7.3:-5003944	MS.gene072664:CDS
TGTGGAGAACTTGGTGGTTA+TGG	0.318822	7.3:-5003974	MS.gene072664:CDS
GAATGAATTTGAGAAAACTA+TGG	0.325001	7.3:-5004173	MS.gene072664:CDS
GGAATAATAGGCTCTTTATC+TGG	0.363048	7.3:-5004214	MS.gene072664:CDS
ATGCAATTGGTCTACATATC+AGG	0.373432	7.3:+5003701	None:intergenic
TTTGAAGGGTATTGTGTTGA+AGG	0.381455	7.3:-5003843	MS.gene072664:CDS
TTTGATGTAGTATAACCATT+AGG	0.390831	7.3:+5004028	None:intergenic
ACAGGTGCAGCCAGTGGAAT+TGG	0.391122	7.3:-5004448	MS.gene072664:CDS
CATATCATCATTGTGATGTC+AGG	0.396278	7.3:-5004315	MS.gene072664:CDS
ACTAGCGTTGCAGCTTCTAT+TGG	0.399254	7.3:-5004061	MS.gene072664:CDS
GGTCATAACTTGGTTGTTGA+TGG	0.406627	7.3:-5003761	MS.gene072664:CDS
ATTGGTGGAACAGGTCCTAA+TGG	0.415583	7.3:-5004043	MS.gene072664:CDS
AAAATCTAGGTTGGAGGGTA+AGG	0.417028	7.3:-5004482	MS.gene072664:intron
GCAGCTTCTATTGGTGGAAC+AGG	0.426744	7.3:-5004052	MS.gene072664:CDS
CGTAAAGTTGCAGATTCAAT+AGG	0.430870	7.3:-5004352	MS.gene072664:CDS
AGAGGTGTTGCAACTCCAAA+AGG	0.435975	7.3:+5003929	None:intergenic
TAGTTCTCATGCTAATTTGA+AGG	0.456961	7.3:-5003858	MS.gene072664:CDS
TTATATCAGTGGTCATAACT+TGG	0.462346	7.3:-5003771	MS.gene072664:CDS
GGTAAGGTGGCAATAATAAC+AGG	0.465773	7.3:-5004466	MS.gene072664:CDS
AGAAACAATTCATTTCACTT+TGG	0.465833	7.3:-5004275	MS.gene072664:CDS
TGTTTCTTCACCAATTCCAC+TGG	0.482489	7.3:+5004438	None:intergenic
TAAGCATGCTGCGCGCGCCA+TGG	0.496972	7.3:-5004119	MS.gene072664:CDS
GTAGACATTCAAGATAAATT+GGG	0.503523	7.3:-5004376	MS.gene072664:CDS
AGCGTTGCAGCTTCTATTGG+TGG	0.515248	7.3:-5004058	MS.gene072664:CDS
TACATATCAGGCGTTGCAGA+TGG	0.522877	7.3:+5003713	None:intergenic
AAATCAGCTTGTGGAGAACT+TGG	0.524096	7.3:-5003983	MS.gene072664:CDS
AGTTCTCATGCTAATTTGAA+GGG	0.524264	7.3:-5003857	MS.gene072664:CDS
CATAACTTGGTTGTTGATGG+TGG	0.529539	7.3:-5003758	MS.gene072664:CDS
GAGGTGTTGCAACTCCAAAA+GGG	0.538111	7.3:+5003930	None:intergenic
ACTATGGCTACAAATGTTGT+TGG	0.550336	7.3:-5004157	MS.gene072664:CDS
TATGACAAAATCTAGGTTGG+AGG	0.554009	7.3:-5004488	MS.gene072664:intron
ATATCATCATTGTGATGTCA+GGG	0.558680	7.3:-5004314	MS.gene072664:CDS
GATGAAGCTGTTTATATCAG+TGG	0.560159	7.3:-5003782	MS.gene072664:CDS
ATGACAAAATCTAGGTTGGA+GGG	0.562378	7.3:-5004487	MS.gene072664:intron
GGACTTGTGAAATCAGCTTG+TGG	0.562826	7.3:-5003992	MS.gene072664:CDS
TTGTAAGCTATACACGAAAG+AGG	0.567482	7.3:+5003911	None:intergenic
TCAGCTTGTGGAGAACTTGG+TGG	0.578232	7.3:-5003980	MS.gene072664:CDS
ATCTAGGTTGGAGGGTAAGG+TGG	0.582293	7.3:-5004479	MS.gene072664:intron
CATTATCAATGATGAAGCAA+AGG	0.601500	7.3:-5004620	None:intergenic
CTACTAGATTCAACTTCATG+AGG	0.605548	7.3:+5003881	None:intergenic
AGGTGTTGCAACTCCAAAAG+GGG	0.641177	7.3:+5003931	None:intergenic
ATAATAACAGGTGCAGCCAG+TGG	0.644904	7.3:-5004454	MS.gene072664:CDS
TAGTGCATATAATCGATCCA+CGG	0.666748	7.3:+5004083	None:intergenic
TGTTGATGGTGGTTTCTCCG+TGG	0.694935	7.3:-5003747	MS.gene072664:CDS
AGATGGAGTATTACGAACCA+CGG	0.715903	7.3:+5003730	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATATATTATGACAAAATCT+AGG	-	chr7.3:5003826-5003845	MS.gene072664:CDS	15.0%
!	AGAAACAATTCATTTCACTT+TGG	-	chr7.3:5004046-5004065	MS.gene072664:CDS	25.0%
!	AGAGACATATATCAAACTAT+AGG	+	chr7.3:5003730-5003749	None:intergenic	25.0%
!	AGTAGACATTCAAGATAAAT+TGG	-	chr7.3:5003944-5003963	MS.gene072664:CDS	25.0%
!	GAATGAATTTGAGAAAACTA+TGG	-	chr7.3:5004148-5004167	MS.gene072664:CDS	25.0%
!	GTAGACATTCAAGATAAATT+GGG	-	chr7.3:5003945-5003964	MS.gene072664:CDS	25.0%
!	TAAGCAGACAAGTAAAAATA+AGG	+	chr7.3:5003770-5003789	None:intergenic	25.0%
!	TATTATGACAAAATCTAGGT+TGG	-	chr7.3:5003830-5003849	MS.gene072664:CDS	25.0%
!	TTTAGTAATGCAGGAATAAT+AGG	-	chr7.3:5004095-5004114	MS.gene072664:CDS	25.0%
!	TTTGATGTAGTATAACCATT+AGG	+	chr7.3:5004296-5004315	None:intergenic	25.0%
!!	AATAACAAATGCTCCATTTT+CGG	+	chr7.3:5003922-5003941	None:intergenic	25.0%
!!!	GATATTTTGTTTAGTAATGC+AGG	-	chr7.3:5004086-5004105	MS.gene072664:CDS	25.0%
	AGTTCTCATGCTAATTTGAA+GGG	-	chr7.3:5004464-5004483	MS.gene072664:CDS	30.0%
	ATATCATCATTGTGATGTCA+GGG	-	chr7.3:5004007-5004026	MS.gene072664:CDS	30.0%
	TAGTTCTCATGCTAATTTGA+AGG	-	chr7.3:5004463-5004482	MS.gene072664:CDS	30.0%
	TTATATCAGTGGTCATAACT+TGG	-	chr7.3:5004550-5004569	MS.gene072664:intron	30.0%
!	ACATCAAAACATGCTCTTTT+AGG	-	chr7.3:5004308-5004327	MS.gene072664:CDS	30.0%
!	CATTTCACTTTGGAAAAACA+CGG	-	chr7.3:5004056-5004075	MS.gene072664:CDS	30.0%
!	GTTAATAGTATATCCCCTTT+TGG	-	chr7.3:5004377-5004396	MS.gene072664:CDS	30.0%
	ACTATGGCTACAAATGTTGT+TGG	-	chr7.3:5004164-5004183	MS.gene072664:CDS	35.0%
	ATGGTTGCTAAAAAAATCCG+TGG	-	chr7.3:5004221-5004240	MS.gene072664:CDS	35.0%
	CATATCATCATTGTGATGTC+AGG	-	chr7.3:5004006-5004025	MS.gene072664:CDS	35.0%
	CGTAAAGTTGCAGATTCAAT+AGG	-	chr7.3:5003969-5003988	MS.gene072664:CDS	35.0%
	CTACTAGATTCAACTTCATG+AGG	+	chr7.3:5004443-5004462	None:intergenic	35.0%
	GATGAAGCTGTTTATATCAG+TGG	-	chr7.3:5004539-5004558	MS.gene072664:intron	35.0%
	GGAATAATAGGCTCTTTATC+TGG	-	chr7.3:5004107-5004126	MS.gene072664:CDS	35.0%
	GTGAAATTGTTTGCCGAAAA+TGG	-	chr7.3:5003906-5003925	MS.gene072664:CDS	35.0%
	TAGTGCATATAATCGATCCA+CGG	+	chr7.3:5004241-5004260	None:intergenic	35.0%
	TTGTAAGCTATACACGAAAG+AGG	+	chr7.3:5004413-5004432	None:intergenic	35.0%
!	ATGACAAAATCTAGGTTGGA+GGG	-	chr7.3:5003834-5003853	MS.gene072664:CDS	35.0%
!	TATGACAAAATCTAGGTTGG+AGG	-	chr7.3:5003833-5003852	MS.gene072664:CDS	35.0%
!!	TTTGAAGGGTATTGTGTTGA+AGG	-	chr7.3:5004478-5004497	MS.gene072664:intron	35.0%
!!!	ACGGATTTTTTTAGCAACCA+TGG	+	chr7.3:5004222-5004241	None:intergenic	35.0%
	AAATCAGCTTGTGGAGAACT+TGG	-	chr7.3:5004338-5004357	MS.gene072664:CDS	40.0%
	GGTAAGGTGGCAATAATAAC+AGG	-	chr7.3:5003855-5003874	MS.gene072664:CDS	40.0%
	GGTCATAACTTGGTTGTTGA+TGG	-	chr7.3:5004560-5004579	MS.gene072664:intron	40.0%
	TGATGTCAGGGATGAAAAAC+AGG	-	chr7.3:5004019-5004038	MS.gene072664:CDS	40.0%
	TGTTTCTTCACCAATTCCAC+TGG	+	chr7.3:5003886-5003905	None:intergenic	40.0%
!	AAAATCTAGGTTGGAGGGTA+AGG	-	chr7.3:5003839-5003858	MS.gene072664:CDS	40.0%
!	AGATGGAGTATTACGAACCA+CGG	+	chr7.3:5004594-5004613	None:intergenic	40.0%
!	CATAACTTGGTTGTTGATGG+TGG	-	chr7.3:5004563-5004582	MS.gene072664:intron	40.0%
	ACTAGCGTTGCAGCTTCTAT+TGG	-	chr7.3:5004260-5004279	MS.gene072664:CDS	45.0%
	ATAATAACAGGTGCAGCCAG+TGG	-	chr7.3:5003867-5003886	MS.gene072664:CDS	45.0%
	ATTGGTGGAACAGGTCCTAA+TGG	-	chr7.3:5004278-5004297	MS.gene072664:CDS	45.0%
	GGACTTGTGAAATCAGCTTG+TGG	-	chr7.3:5004329-5004348	MS.gene072664:CDS	45.0%
!	AGGTGTTGCAACTCCAAAAG+GGG	+	chr7.3:5004393-5004412	None:intergenic	45.0%
!	TACATATCAGGCGTTGCAGA+TGG	+	chr7.3:5004611-5004630	None:intergenic	45.0%
!	TGTGGAGAACTTGGTGGTTA+TGG	-	chr7.3:5004347-5004366	MS.gene072664:CDS	45.0%
!!	AGAGGTGTTGCAACTCCAAA+AGG	+	chr7.3:5004395-5004414	None:intergenic	45.0%
!!	GAGGTGTTGCAACTCCAAAA+GGG	+	chr7.3:5004394-5004413	None:intergenic	45.0%
	AGCGTTGCAGCTTCTATTGG+TGG	-	chr7.3:5004263-5004282	MS.gene072664:CDS	50.0%
	GCAGCTTCTATTGGTGGAAC+AGG	-	chr7.3:5004269-5004288	MS.gene072664:CDS	50.0%
	TCAGCTTGTGGAGAACTTGG+TGG	-	chr7.3:5004341-5004360	MS.gene072664:CDS	50.0%
!	ATCTAGGTTGGAGGGTAAGG+TGG	-	chr7.3:5003842-5003861	MS.gene072664:CDS	50.0%
!!	TGTTGATGGTGGTTTCTCCG+TGG	-	chr7.3:5004574-5004593	MS.gene072664:intron	50.0%
	ACAGGTGCAGCCAGTGGAAT+TGG	-	chr7.3:5003873-5003892	MS.gene072664:CDS	55.0%
	TAAGCATGCTGCGCGCGCCA+TGG	-	chr7.3:5004202-5004221	MS.gene072664:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	5003712	5004631	5003712	ID=MS.gene072664
chr7.3	mRNA	5003712	5004631	5003712	ID=MS.gene072664.t1;Parent=MS.gene072664
chr7.3	exon	5004621	5004631	5004621	ID=MS.gene072664.t1.exon1;Parent=MS.gene072664.t1
chr7.3	CDS	5004621	5004631	5004621	ID=cds.MS.gene072664.t1;Parent=MS.gene072664.t1
chr7.3	exon	5003712	5004495	5003712	ID=MS.gene072664.t1.exon2;Parent=MS.gene072664.t1
chr7.3	CDS	5003712	5004495	5003712	ID=cds.MS.gene072664.t1;Parent=MS.gene072664.t1

Gene Sequence

>MS.gene072664

ATGAAGCAAAGGTATCCTATAGTTTGATATATGTCTCTATAGTTTTGTTCTGTTTCCTTATTTTTACTTGTCTGCTTAATTTTGATTAATTGACTTTATTTTATTTTCTTGCATAATATATTATGACAAAATCTAGGTTGGAGGGTAAGGTGGCAATAATAACAGGTGCAGCCAGTGGAATTGGTGAAGAAACAGTGAAATTGTTTGCCGAAAATGGAGCATTTGTTATTGCAGTAGACATTCAAGATAAATTGGGTCGTAAAGTTGCAGATTCAATAGGCTCAGATAAAGTAACATATCATCATTGTGATGTCAGGGATGAAAAACAGGTCGAAGAAACAATTCATTTCACTTTGGAAAAACACGGTTGCATCGATATTTTGTTTAGTAATGCAGGAATAATAGGCTCTTTATCTGGAATACTTGATCTTGATTTGAATGAATTTGAGAAAACTATGGCTACAAATGTTGTTGGTGCAGCTGCTACAATTAAGCATGCTGCGCGCGCCATGGTTGCTAAAAAAATCCGTGGATCGATTATATGCACTACTAGCGTTGCAGCTTCTATTGGTGGAACAGGTCCTAATGGTTATACTACATCAAAACATGCTCTTTTAGGACTTGTGAAATCAGCTTGTGGAGAACTTGGTGGTTATGGAATTAGAGTTAATAGTATATCCCCTTTTGGAGTTGCAACACCTCTTTCGTGTATAGCTTACAATCTTGAACCTCATGAAGTTGAATCTAGTAGTAGTTCTCATGCTAATTTGAAGGGTATTGTGTTGAAGGCTAAGCATGTTGCAGAAGCAGCTTTGTTTCTTGCTTCTGATGAAGCTGTTTATATCAGTGGTCATAACTTGGTTGTTGATGGTGGTTTCTCCGTGGTTCGTAATACTCCATCTGCAACGCCTGATATGTAG

Protein sequence

>MS.gene072664.t1

MKQRLEGKVAIITGAASGIGEETVKLFAENGAFVIAVDIQDKLGRKVADSIGSDKVTYHHCDVRDEKQVEETIHFTLEKHGCIDILFSNAGIIGSLSGILDLDLNEFEKTMATNVVGAAATIKHAARAMVAKKIRGSIICTTSVAASIGGTGPNGYTTSKHALLGLVKSACGELGGYGIRVNSISPFGVATPLSCIAYNLEPHEVESSSSSHANLKGIVLKAKHVAEAALFLASDEAVYISGHNLVVDGGFSVVRNTPSATPDM