AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85724

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85724.t1 MTR_6g035395 87.739 261 19 1 2 262 1 248 3.51e-161 447
MS.gene85724.t1 MTR_7g110140 77.043 257 59 0 1 257 1 257 5.28e-144 404
MS.gene85724.t1 MTR_7g110130 68.992 258 79 1 1 257 1 258 1.33e-125 358
MS.gene85724.t1 MTR_2g090165 66.926 257 85 0 1 257 1 257 6.07e-124 353
MS.gene85724.t1 MTR_7g110160 62.257 257 79 1 1 257 1 239 2.27e-107 311
MS.gene85724.t1 MTR_6g023590 51.394 251 120 2 5 253 12 262 1.83e-85 256
MS.gene85724.t1 MTR_6g023950 50.988 253 122 2 5 255 12 264 5.32e-81 244
MS.gene85724.t1 MTR_4g052350 52.157 255 117 3 4 257 12 262 6.00e-80 242
MS.gene85724.t1 MTR_6g023610 50.400 250 122 2 5 252 12 261 1.36e-79 241
MS.gene85724.t1 MTR_6g023990 50.199 251 123 2 5 253 13 263 1.77e-79 241
MS.gene85724.t1 MTR_4g052340 50.588 255 121 3 4 257 12 262 3.78e-79 240
MS.gene85724.t1 MTR_6g023910 51.200 250 120 2 5 252 12 261 4.96e-79 239
MS.gene85724.t1 MTR_6g023600 50.996 251 121 2 5 253 12 262 1.45e-78 238
MS.gene85724.t1 MTR_4g052320 50.787 254 121 3 5 257 7 257 1.49e-76 233
MS.gene85724.t1 MTR_7g071680 47.810 274 121 4 4 257 27 298 4.30e-76 233
MS.gene85724.t1 MTR_4g052300 50.394 254 121 3 5 257 7 256 5.36e-75 229
MS.gene85724.t1 MTR_7g105460 47.431 253 131 2 5 256 12 263 7.28e-74 226
MS.gene85724.t1 MTR_6g023600 50.199 251 117 3 5 253 12 256 1.39e-73 225
MS.gene85724.t1 MTR_7g107670 45.247 263 134 6 4 259 31 290 4.10e-69 215
MS.gene85724.t1 MTR_7g083870 47.510 261 128 5 4 257 33 291 5.11e-69 215
MS.gene85724.t1 MTR_7g105580 45.059 253 121 3 4 256 6 240 2.88e-63 199
MS.gene85724.t1 MTR_4g078630 42.697 267 142 5 4 262 17 280 6.10e-62 196
MS.gene85724.t1 MTR_3g020670 45.660 265 134 5 4 259 14 277 1.66e-59 190
MS.gene85724.t1 MTR_7g105590 47.568 185 95 2 4 187 6 189 6.81e-53 170
MS.gene85724.t1 MTR_3g113870 42.308 182 102 3 14 194 29 208 8.03e-44 148
MS.gene85724.t1 MTR_7g105570 36.905 252 106 3 6 256 21 220 2.36e-42 144
MS.gene85724.t1 MTR_4g084800 47.143 140 73 1 2 140 9 148 5.91e-39 133
MS.gene85724.t1 MTR_4g058840 28.195 266 162 7 6 254 11 264 1.93e-21 90.9
MS.gene85724.t1 MTR_4g058820 28.195 266 162 7 6 254 11 264 1.93e-21 90.9
MS.gene85724.t1 MTR_3g085740 29.297 256 164 8 4 252 73 318 2.12e-20 89.0
MS.gene85724.t1 MTR_1g051165 26.515 264 178 7 4 254 12 272 2.52e-20 88.2
MS.gene85724.t1 MTR_8g442750 31.321 265 151 11 6 252 14 265 1.66e-18 83.6
MS.gene85724.t1 MTR_4g132850 29.134 254 169 7 2 252 3 248 8.33e-18 80.5
MS.gene85724.t1 MTR_4g058810 27.170 265 166 8 6 254 11 264 4.05e-17 79.3
MS.gene85724.t1 MTR_7g113650 28.405 257 167 6 5 251 39 288 8.62e-17 78.6
MS.gene85724.t1 MTR_1g082760 27.444 266 158 7 5 251 39 288 2.19e-16 77.4
MS.gene85724.t1 MTR_4g097510 31.694 183 119 5 7 187 3 181 4.13e-16 76.6
MS.gene85724.t1 MTR_4g131740 25.954 262 168 8 5 251 39 289 1.12e-15 75.5
MS.gene85724.t1 MTR_7g105560 58.621 58 24 0 2 59 9 66 1.43e-15 72.4
MS.gene85724.t1 MTR_4g064887 25.969 258 177 7 3 254 12 261 2.04e-15 74.3
MS.gene85724.t1 MTR_2g022600 31.416 226 138 7 12 231 39 253 6.87e-15 73.9
MS.gene85724.t1 MTR_3g068085 28.571 252 172 8 6 254 15 261 1.74e-14 71.6
MS.gene85724.t1 MTR_1196s0010 31.250 256 149 10 7 252 275 513 4.44e-14 72.0
MS.gene85724.t1 MTR_8g096580 30.612 196 129 4 4 195 43 235 7.45e-14 70.5
MS.gene85724.t1 MTR_3g087850 30.556 180 117 5 10 187 30 203 2.59e-13 68.9
MS.gene85724.t1 MTR_4g086400 24.419 258 160 7 8 248 6 245 6.37e-13 68.6
MS.gene85724.t1 MTR_7g094520 27.953 254 171 7 6 252 11 259 1.22e-12 66.2
MS.gene85724.t1 MTR_7g063390 28.077 260 173 8 3 254 13 266 2.33e-12 65.9
MS.gene85724.t1 MTR_7g094510 25.856 263 175 8 1 252 1 254 1.91e-11 62.8
MS.gene85724.t1 MTR_7g063320 27.843 255 176 7 3 252 12 263 2.66e-11 62.8
MS.gene85724.t1 MTR_3g087860 33.835 133 84 3 56 187 59 188 2.71e-11 62.8
MS.gene85724.t1 MTR_4g066050 28.889 180 108 5 9 175 27 199 4.63e-11 62.0
MS.gene85724.t1 MTR_4g086410 23.922 255 159 7 11 248 9 245 5.18e-11 62.8
MS.gene85724.t1 MTR_3g068175 27.344 256 176 8 3 254 14 263 9.43e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85724.t1 AT2G47140 61.176 255 99 0 1 255 1 255 2.69e-112 323
MS.gene85724.t1 AT3G29250 61.176 255 99 0 1 255 1 255 1.09e-105 307
MS.gene85724.t1 AT3G29250 61.024 254 99 0 2 255 40 293 2.50e-105 307
MS.gene85724.t1 AT3G29250 61.176 255 99 0 1 255 37 291 3.87e-105 307
MS.gene85724.t1 AT3G29260 58.431 255 105 1 1 255 1 254 1.99e-100 293
MS.gene85724.t1 AT2G47130 56.977 258 110 1 1 258 1 257 5.57e-99 290
MS.gene85724.t1 AT2G47120 56.175 251 108 2 5 255 6 254 8.97e-96 282
MS.gene85724.t1 AT2G47120 55.686 255 111 2 1 255 1 253 9.67e-96 281
MS.gene85724.t1 AT3G51680 47.253 273 128 3 1 257 27 299 8.90e-74 227
MS.gene85724.t1 AT1G52340 45.522 268 133 6 4 259 16 282 1.15e-64 203
MS.gene85724.t1 AT4G03140 42.912 261 141 5 4 258 76 334 1.14e-62 200
MS.gene85724.t1 AT3G26760 40.909 264 145 4 4 258 34 295 5.81e-62 197
MS.gene85724.t1 AT3G26770 40.856 257 143 4 4 253 39 293 3.41e-60 192
MS.gene85724.t1 AT3G42960 42.857 259 136 4 4 253 6 261 4.32e-54 176
MS.gene85724.t1 AT2G47150 37.945 253 104 6 6 258 1 200 1.17e-39 137
MS.gene85724.t1 AT2G17845 28.676 272 158 9 6 255 47 304 4.30e-18 82.4
MS.gene85724.t1 AT4G05530 31.907 257 158 7 2 252 5 250 4.34e-18 81.6
MS.gene85724.t1 AT3G12800 30.153 262 163 9 6 254 10 264 5.27e-17 79.3
MS.gene85724.t1 AT2G30670 28.906 256 172 7 3 254 4 253 1.24e-16 77.8
MS.gene85724.t1 AT3G55290 28.090 267 161 8 6 254 17 270 1.83e-16 77.4
MS.gene85724.t1 AT3G55290 28.090 267 161 8 6 254 18 271 1.84e-16 77.4
MS.gene85724.t1 AT2G29150 31.818 264 153 12 3 254 13 261 2.50e-16 77.0
MS.gene85724.t1 AT2G29340 29.020 255 173 6 3 254 4 253 3.76e-16 76.3
MS.gene85724.t1 AT1G07440 28.794 257 170 8 3 254 9 257 4.05e-16 76.3
MS.gene85724.t1 AT2G29340 29.020 255 173 6 3 254 4 253 5.67e-16 76.6
MS.gene85724.t1 AT1G63380 27.341 267 164 9 6 254 22 276 7.84e-16 75.9
MS.gene85724.t1 AT1G07450 28.794 257 170 8 3 254 5 253 8.23e-16 75.5
MS.gene85724.t1 AT1G63380 27.341 267 164 9 6 254 19 273 8.45e-16 75.9
MS.gene85724.t1 AT2G29370 30.451 266 155 11 3 254 13 262 8.60e-16 75.5
MS.gene85724.t1 AT1G63380 27.341 267 164 9 6 254 17 271 8.94e-16 75.5
MS.gene85724.t1 AT2G29310 28.405 257 172 7 3 254 4 253 9.52e-16 75.1
MS.gene85724.t1 AT1G63380 27.341 267 164 9 6 254 15 269 1.01e-15 75.5
MS.gene85724.t1 AT2G29290 30.075 266 156 9 3 254 4 253 1.27e-15 74.7
MS.gene85724.t1 AT3G55310 28.409 264 158 8 6 251 17 267 1.91e-15 74.7
MS.gene85724.t1 AT1G62610 27.027 259 169 7 6 251 13 264 2.48e-15 74.3
MS.gene85724.t1 AT1G62610 27.027 259 169 7 6 251 14 265 2.82e-15 73.9
MS.gene85724.t1 AT1G62610 27.027 259 169 7 6 251 15 266 2.91e-15 73.9
MS.gene85724.t1 AT1G62610 27.027 259 169 7 6 251 19 270 3.02e-15 73.9
MS.gene85724.t1 AT2G29360 30.075 266 156 11 3 254 13 262 9.61e-15 72.4
MS.gene85724.t1 AT2G29330 28.794 257 171 8 3 254 4 253 1.06e-14 72.4
MS.gene85724.t1 AT5G06060 28.571 259 169 8 3 254 6 255 1.48e-14 72.0
MS.gene85724.t1 AT1G54870 27.586 261 164 7 5 251 83 332 3.00e-14 72.0
MS.gene85724.t1 AT1G54870 27.586 261 164 7 5 251 81 330 3.11e-14 71.6
MS.gene85724.t1 AT1G49670 29.493 217 126 7 11 209 9 216 3.38e-14 72.4
MS.gene85724.t1 AT1G49670 29.493 217 126 7 11 209 9 216 3.70e-14 72.4
MS.gene85724.t1 AT5G50600 30.000 190 127 4 1 187 40 226 9.05e-14 70.5
MS.gene85724.t1 AT5G50700 30.000 190 127 4 1 187 40 226 9.05e-14 70.5
MS.gene85724.t1 AT3G46170 26.136 264 164 8 6 251 26 276 1.24e-13 69.7
MS.gene85724.t1 AT5G65205 29.787 188 124 5 7 192 7 188 2.40e-13 68.9
MS.gene85724.t1 AT5G18210 29.845 258 164 8 6 252 8 259 4.92e-13 67.8
MS.gene85724.t1 AT2G29260 26.415 265 167 7 3 254 65 314 6.93e-13 67.8
MS.gene85724.t1 AT2G29320 29.344 259 162 8 6 254 13 260 7.60e-13 67.0
MS.gene85724.t1 AT5G10050 28.571 189 125 6 7 192 6 187 9.85e-13 67.0
MS.gene85724.t1 AT4G13180 30.233 258 164 7 6 254 13 263 1.57e-12 66.2
MS.gene85724.t1 AT5G19200 31.604 212 133 5 12 217 41 246 1.95e-12 66.6
MS.gene85724.t1 AT2G29290 29.630 216 133 6 42 254 37 236 3.06e-12 65.1
MS.gene85724.t1 AT3G05260 25.287 261 175 8 5 254 36 287 3.72e-12 65.5
MS.gene85724.t1 AT1G10310 29.412 170 114 3 9 175 18 184 5.52e-12 64.3
MS.gene85724.t1 AT3G06060 29.279 222 145 5 2 217 33 248 7.80e-12 64.7
MS.gene85724.t1 AT3G06060 29.279 222 145 5 2 217 50 265 7.97e-12 64.7
MS.gene85724.t1 AT5G50770 30.000 190 127 4 1 187 40 226 1.19e-11 64.3
MS.gene85724.t1 AT2G29340 29.082 196 134 4 3 195 4 197 1.96e-11 62.0
MS.gene85724.t1 AT2G29340 29.082 196 134 4 3 195 4 197 1.96e-11 62.0
MS.gene85724.t1 AT2G29310 28.788 198 132 5 3 195 4 197 6.67e-11 60.5

Find 53 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACATCAGTGGTCATAATT+TGG 0.260914 6.1:-16682444 MS.gene85724:CDS
GTGAAGTTGTTCGTCGAAAA+TGG 0.282900 6.1:-16683088 MS.gene85724:CDS
CATCAAGTTGTTTCTTCAAT+AGG 0.284749 6.1:-16683025 MS.gene85724:CDS
TTCTTGAACATTTAGGTTAG+AGG 0.321097 6.1:-16683161 MS.gene85724:intron
TTCAGCAATGCCGGAATATT+AGG 0.326721 6.1:-16682899 MS.gene85724:CDS
GTAGACATTCAAGATGAATT+AGG 0.344204 6.1:-16683049 MS.gene85724:CDS
GTTAATAGTGTTTCCCCTTT+CGG 0.349469 6.1:-16682617 MS.gene85724:CDS
GGAAGCGATCGTAAGTTCTA+AGG 0.349654 6.1:-16682543 MS.gene85724:CDS
TGTAGTGAACTTGGTGGTTA+TGG 0.359732 6.1:-16682647 MS.gene85724:CDS
CGGAATATTAGGCTCTTCAT+TGG 0.389239 6.1:-16682888 MS.gene85724:CDS
CAATGAAGAGCCTAATATTC+CGG 0.395683 6.1:+16682889 None:intergenic
GGTCATAATTTGGTGGTTGA+TGG 0.405463 6.1:-16682434 MS.gene85724:CDS
CATACCATCATTGCGACGTT+AGG 0.415601 6.1:-16682988 MS.gene85724:CDS
ACGATCGCTTCCACAACTTC+CGG 0.440285 6.1:+16682554 None:intergenic
TCTGAAGGGTGTTGTGTTGA+AGG 0.448691 6.1:-16682516 MS.gene85724:CDS
ACTGGTGCAGCCAGTGGAAT+TGG 0.449174 6.1:-16683121 MS.gene85724:CDS
ACAATAGATATAAATGTTGT+CGG 0.460253 6.1:-16682830 MS.gene85724:CDS
GGTTCATAATATAAAACAAA+TGG 0.461191 6.1:-16682399 MS.gene85724:CDS
CATCCCTAACGTCGCAATGA+TGG 0.461260 6.1:+16682984 None:intergenic
GGAATATTAGGCTCTTCATT+GGG 0.481611 6.1:-16682887 MS.gene85724:CDS
GCAAGTGTGGCAGCTTCTAC+CGG 0.482390 6.1:-16682734 MS.gene85724:CDS
AGAAACTGTTAATTTCACTT+TGG 0.484629 6.1:-16682948 MS.gene85724:CDS
CAAAATAGAGCCGGAAGTTG+TGG 0.492410 6.1:-16682564 MS.gene85724:CDS
ATACCATCATTGCGACGTTA+GGG 0.497406 6.1:-16682987 MS.gene85724:CDS
GATATCATGTTCAGCAATGC+CGG 0.499855 6.1:-16682908 MS.gene85724:CDS
TCTTGAACATTTAGGTTAGA+GGG 0.503233 6.1:-16683160 MS.gene85724:intron
CATAATTTGGTGGTTGATGG+TGG 0.514887 6.1:-16682431 MS.gene85724:CDS
TGCCTCTTCACCAATTCCAC+TGG 0.515824 6.1:+16683111 None:intergenic
CATCAGTGGTCATAATTTGG+TGG 0.524756 6.1:-16682441 MS.gene85724:CDS
TAAGCACGCTGCTCGTGCTA+TGG 0.527417 6.1:-16682792 MS.gene85724:CDS
TTGATCGGTGCACACGCGAA+AGG 0.535273 6.1:+16682584 None:intergenic
GCGGTGCATATGATCGATCC+AGG 0.538975 6.1:+16682755 None:intergenic
AAGTTCTAAGGGTAATCTGA+AGG 0.564554 6.1:-16682531 MS.gene85724:CDS
AAGGTGTTGCAACTCCGAAA+GGG 0.569228 6.1:+16682603 None:intergenic
AAAGGTGTTGCAACTCCGAA+AGG 0.570889 6.1:+16682602 None:intergenic
ACCACTAGGATCAGCTCCAC+CGG 0.570926 6.1:+16682715 None:intergenic
CACCGTTGATGATGAAGCAA+AGG 0.574370 6.1:-16683328 None:intergenic
GATGAAGCTGTTTACATCAG+TGG 0.575837 6.1:-16682455 MS.gene85724:CDS
AGAGCAGCATGTAGTGAACT+TGG 0.584268 6.1:-16682656 MS.gene85724:CDS
GAAGCGATCGTAAGTTCTAA+GGG 0.587496 6.1:-16682542 MS.gene85724:CDS
TGCACCGATCAAAATAGAGC+CGG 0.591663 6.1:-16682573 MS.gene85724:CDS
GGTAAGATAGCAATCATCAC+TGG 0.605060 6.1:-16683139 MS.gene85724:CDS
ATCATCACTGGTGCAGCCAG+TGG 0.607127 6.1:-16683127 MS.gene85724:CDS
AGTTCTAAGGGTAATCTGAA+GGG 0.611602 6.1:-16682530 MS.gene85724:CDS
GATCATATGCACCGCAAGTG+TGG 0.612160 6.1:-16682747 MS.gene85724:CDS
AGCCAGTGGAATTGGTGAAG+AGG 0.612587 6.1:-16683113 MS.gene85724:CDS
GCAGCATGTAGTGAACTTGG+TGG 0.616204 6.1:-16682653 MS.gene85724:CDS
AGTGTGGCAGCTTCTACCGG+TGG 0.617157 6.1:-16682731 MS.gene85724:CDS
GGTAGAAGCTGCCACACTTG+CGG 0.622506 6.1:+16682736 None:intergenic
GGTTGATGGTGGCTTCTCTG+TGG 0.628769 6.1:-16682420 MS.gene85724:CDS
AGGTGTTGCAACTCCGAAAG+GGG 0.633333 6.1:+16682604 None:intergenic
TTTGATGCAGTATAACCACT+AGG 0.633782 6.1:+16682701 None:intergenic
ACCGGTGGAGCTGATCCTAG+TGG 0.662113 6.1:-16682716 MS.gene85724:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAATAGATATAAATGTTGT+CGG - chr6.1:16682884-16682903 MS.gene85724:CDS 20.0%
!! GGTTCATAATATAAAACAAA+TGG - chr6.1:16683315-16683334 MS.gene85724:intron 20.0%
!! TCAAGAAAAACTAATCTAAA+GGG + chr6.1:16682461-16682480 None:intergenic 20.0%
!! TTCAAGAAAAACTAATCTAA+AGG + chr6.1:16682462-16682481 None:intergenic 20.0%
!!! TTTTTCTTTCTTGAACATTT+AGG - chr6.1:16682546-16682565 MS.gene85724:CDS 20.0%
! AGAAACTGTTAATTTCACTT+TGG - chr6.1:16682766-16682785 MS.gene85724:CDS 25.0%
! AGATACACACACATATATAT+AGG + chr6.1:16682416-16682435 None:intergenic 25.0%
! CAAGAAAAACTAATCTAAAG+GGG + chr6.1:16682460-16682479 None:intergenic 25.0%
! GATACACACACATATATATA+GGG + chr6.1:16682415-16682434 None:intergenic 25.0%
!! AATTTCACTTTGGAAAAACA+TGG - chr6.1:16682776-16682795 MS.gene85724:CDS 25.0%
!!! ATATGTGTGTGTATCTTTTT+TGG - chr6.1:16682420-16682439 MS.gene85724:CDS 25.0%
GTAGACATTCAAGATGAATT+AGG - chr6.1:16682665-16682684 MS.gene85724:CDS 30.0%
TTACATCAGTGGTCATAATT+TGG - chr6.1:16683270-16683289 MS.gene85724:intron 30.0%
! TCTTGAACATTTAGGTTAGA+GGG - chr6.1:16682554-16682573 MS.gene85724:CDS 30.0%
! TTCTTGAACATTTAGGTTAG+AGG - chr6.1:16682553-16682572 MS.gene85724:CDS 30.0%
!! CATCAAGTTGTTTCTTCAAT+AGG - chr6.1:16682689-16682708 MS.gene85724:CDS 30.0%
ATGGTTGCTAAAAATGTTCC+TGG - chr6.1:16682941-16682960 MS.gene85724:CDS 35.0%
CAATGAAGAGCCTAATATTC+CGG + chr6.1:16682828-16682847 None:intergenic 35.0%
GGAATATTAGGCTCTTCATT+GGG - chr6.1:16682827-16682846 MS.gene85724:CDS 35.0%
GTTAATAGTGTTTCCCCTTT+CGG - chr6.1:16683097-16683116 MS.gene85724:CDS 35.0%
TTTGATGCAGTATAACCACT+AGG + chr6.1:16683016-16683035 None:intergenic 35.0%
! GCATCAAAACATGCTCTTTT+AGG - chr6.1:16683028-16683047 MS.gene85724:CDS 35.0%
!! AAGTTCTAAGGGTAATCTGA+AGG - chr6.1:16683183-16683202 MS.gene85724:intron 35.0%
!! AGTTCTAAGGGTAATCTGAA+GGG - chr6.1:16683184-16683203 MS.gene85724:intron 35.0%
!!! AAAACATGCTCTTTTAGGAC+TGG - chr6.1:16683033-16683052 MS.gene85724:CDS 35.0%
ATACCATCATTGCGACGTTA+GGG - chr6.1:16682727-16682746 MS.gene85724:CDS 40.0%
CATCAGTGGTCATAATTTGG+TGG - chr6.1:16683273-16683292 MS.gene85724:intron 40.0%
CGGAATATTAGGCTCTTCAT+TGG - chr6.1:16682826-16682845 MS.gene85724:CDS 40.0%
GATATCATGTTCAGCAATGC+CGG - chr6.1:16682806-16682825 MS.gene85724:CDS 40.0%
GATGAAGCTGTTTACATCAG+TGG - chr6.1:16683259-16683278 MS.gene85724:intron 40.0%
GGTAAGATAGCAATCATCAC+TGG - chr6.1:16682575-16682594 MS.gene85724:CDS 40.0%
GTGAAGTTGTTCGTCGAAAA+TGG - chr6.1:16682626-16682645 MS.gene85724:CDS 40.0%
TTCAGCAATGCCGGAATATT+AGG - chr6.1:16682815-16682834 MS.gene85724:CDS 40.0%
! GAAGCGATCGTAAGTTCTAA+GGG - chr6.1:16683172-16683191 MS.gene85724:intron 40.0%
! GGTCATAATTTGGTGGTTGA+TGG - chr6.1:16683280-16683299 MS.gene85724:intron 40.0%
! TGTAGTGAACTTGGTGGTTA+TGG - chr6.1:16683067-16683086 MS.gene85724:CDS 40.0%
!! CATAATTTGGTGGTTGATGG+TGG - chr6.1:16683283-16683302 MS.gene85724:intron 40.0%
!!! ACTTCCGGCTCTATTTTGAT+CGG + chr6.1:16683148-16683167 None:intergenic 40.0%
AGAGCAGCATGTAGTGAACT+TGG - chr6.1:16683058-16683077 MS.gene85724:CDS 45.0%
CAAAATAGAGCCGGAAGTTG+TGG - chr6.1:16683150-16683169 MS.gene85724:intron 45.0%
CATACCATCATTGCGACGTT+AGG - chr6.1:16682726-16682745 MS.gene85724:CDS 45.0%
CGACGTTAGGGATGAAAAAC+AGG - chr6.1:16682739-16682758 MS.gene85724:CDS 45.0%
GGAAGCGATCGTAAGTTCTA+AGG - chr6.1:16683171-16683190 MS.gene85724:intron 45.0%
! TGCACCGATCAAAATAGAGC+CGG - chr6.1:16683141-16683160 MS.gene85724:CDS 45.0%
!! AAAGGTGTTGCAACTCCGAA+AGG + chr6.1:16683115-16683134 None:intergenic 45.0%
!! AAGGTGTTGCAACTCCGAAA+GGG + chr6.1:16683114-16683133 None:intergenic 45.0%
!! TCTGAAGGGTGTTGTGTTGA+AGG - chr6.1:16683198-16683217 MS.gene85724:intron 45.0%
ACGATCGCTTCCACAACTTC+CGG + chr6.1:16683163-16683182 None:intergenic 50.0%
AGCCAGTGGAATTGGTGAAG+AGG - chr6.1:16682601-16682620 MS.gene85724:CDS 50.0%
CATCCCTAACGTCGCAATGA+TGG + chr6.1:16682733-16682752 None:intergenic 50.0%
GATCATATGCACCGCAAGTG+TGG - chr6.1:16682967-16682986 MS.gene85724:CDS 50.0%
GCAGCATGTAGTGAACTTGG+TGG - chr6.1:16683061-16683080 MS.gene85724:CDS 50.0%
TGCCTCTTCACCAATTCCAC+TGG + chr6.1:16682606-16682625 None:intergenic 50.0%
! AGGTGTTGCAACTCCGAAAG+GGG + chr6.1:16683113-16683132 None:intergenic 50.0%
ACCACTAGGATCAGCTCCAC+CGG + chr6.1:16683002-16683021 None:intergenic 55.0%
ACTGGTGCAGCCAGTGGAAT+TGG - chr6.1:16682593-16682612 MS.gene85724:CDS 55.0%
ATCATCACTGGTGCAGCCAG+TGG - chr6.1:16682587-16682606 MS.gene85724:CDS 55.0%
GCAAGTGTGGCAGCTTCTAC+CGG - chr6.1:16682980-16682999 MS.gene85724:CDS 55.0%
GCGGTGCATATGATCGATCC+AGG + chr6.1:16682962-16682981 None:intergenic 55.0%
GGTAGAAGCTGCCACACTTG+CGG + chr6.1:16682981-16683000 None:intergenic 55.0%
TAAGCACGCTGCTCGTGCTA+TGG - chr6.1:16682922-16682941 MS.gene85724:CDS 55.0%
TTGATCGGTGCACACGCGAA+AGG + chr6.1:16683133-16683152 None:intergenic 55.0%
!! GGTTGATGGTGGCTTCTCTG+TGG - chr6.1:16683294-16683313 MS.gene85724:intron 55.0%
ACCGGTGGAGCTGATCCTAG+TGG - chr6.1:16682998-16683017 MS.gene85724:CDS 60.0%
AGTGTGGCAGCTTCTACCGG+TGG - chr6.1:16682983-16683002 MS.gene85724:CDS 60.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 16682394 16683342 16682394 ID=MS.gene85724
chr6.1 mRNA 16682394 16683342 16682394 ID=MS.gene85724.t1;Parent=MS.gene85724
chr6.1 exon 16683329 16683342 16683329 ID=MS.gene85724.t1.exon1;Parent=MS.gene85724.t1
chr6.1 CDS 16683329 16683342 16683329 ID=cds.MS.gene85724.t1;Parent=MS.gene85724.t1
chr6.1 exon 16682394 16683168 16682394 ID=MS.gene85724.t1.exon2;Parent=MS.gene85724.t1
chr6.1 CDS 16682394 16683168 16682394 ID=cds.MS.gene85724.t1;Parent=MS.gene85724.t1
Gene Sequence

>MS.gene85724

ATGATGAAGCAAAGGTATCCCTATATATATGTGTGTGTATCTTTTTTGGTGTAATATTTTTTTCCCCTTTAGATTAGTTTTTCTTGAAAATTGTTCATTCTAATTTGCACTTTTCAGCTCTATATATTTTGATTAGTTGACTTTACTTTTTTTTTTTCTTTCTTGAACATTTAGGTTAGAGGGTAAGATAGCAATCATCACTGGTGCAGCCAGTGGAATTGGTGAAGAGGCAGTGAAGTTGTTCGTCGAAAATGGAGCATTTGTTGTTGCAGTAGACATTCAAGATGAATTAGGTCATCAAGTTGTTTCTTCAATAGGCTCAAATAAAGTGACATACCATCATTGCGACGTTAGGGATGAAAAACAGGTTGAAGAAACTGTTAATTTCACTTTGGAAAAACATGGTCAACTTGATATCATGTTCAGCAATGCCGGAATATTAGGCTCTTCATTGGGCGTACTCGAGTTCGATCTAAATGAATTCAAGAACACAATAGATATAAATGTTGTCGGTTCAGCTGCTATGATTAAGCACGCTGCTCGTGCTATGGTTGCTAAAAATGTTCCTGGATCGATCATATGCACCGCAAGTGTGGCAGCTTCTACCGGTGGAGCTGATCCTAGTGGTTATACTGCATCAAAACATGCTCTTTTAGGACTGGTGAGAGCAGCATGTAGTGAACTTGGTGGTTATGGAATAAGAGTTAATAGTGTTTCCCCTTTCGGAGTTGCAACACCTTTCGCGTGTGCACCGATCAAAATAGAGCCGGAAGTTGTGGAAGCGATCGTAAGTTCTAAGGGTAATCTGAAGGGTGTTGTGTTGAAGGCTAAGCATATTGCAGAAGCAGCTTTGTTTCTTGCTTCTGATGAAGCTGTTTACATCAGTGGTCATAATTTGGTGGTTGATGGTGGCTTCTCTGTGGTTCATAATATAAAACAAATGGTTTAA
Protein sequence

>MS.gene85724.t1

MMKQRLEGKIAIITGAASGIGEEAVKLFVENGAFVVAVDIQDELGHQVVSSIGSNKVTYHHCDVRDEKQVEETVNFTLEKHGQLDIMFSNAGILGSSLGVLEFDLNEFKNTIDINVVGSAAMIKHAARAMVAKNVPGSIICTASVAASTGGADPSGYTASKHALLGLVRAACSELGGYGIRVNSVSPFGVATPFACAPIKIEPEVVEAIVSSKGNLKGVVLKAKHIAEAALFLASDEAVYISGHNLVVDGGFSVVHNIKQMV